CN101928745A - Preparation method and applications of ant polypeptide - Google Patents
Preparation method and applications of ant polypeptide Download PDFInfo
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- CN101928745A CN101928745A CN2010102521185A CN201010252118A CN101928745A CN 101928745 A CN101928745 A CN 101928745A CN 2010102521185 A CN2010102521185 A CN 2010102521185A CN 201010252118 A CN201010252118 A CN 201010252118A CN 101928745 A CN101928745 A CN 101928745A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention relates to a preparation method and applications of ant polypeptide. The invention mainly overcomes the defects of difficult digestion and absorption and poor processing performance of ant protein. The method comprises the following steps: firstly, cleaning ants, drying the ants at low temperature, and crushing and sieving the ants to prepare dry ant powder; carrying out degreasing treatment by utilizing a supercritical CO2 extraction technology to prepare the ant protein; carrying out enzymolysis on the degreased ant protein by protease; then inactivating enzymes, sterilizing and filtering; carrying out vacuum concentration; and freeze-drying or spray-drying to obtain ant polypeptide powder. The ant polypeptide prepared according to the invention has stable properties and good processing performance, can be stored easily, and can be widely used for medicines, health care products, food raw materials or food additives; and because the ant polypeptide has low cost, less investment, high effect taking speed and high additional value, the ant polypeptide is suitable for industrialized production.
Description
Technical field
The present invention relates to belong to biological technical field, relate in particular to a kind of Preparation method and use of ant polypeptide.
Background technology
Ant belongs to Arthropoda Insecta Hymenoptera Formicidae, and it is of a great variety, and the whole world has kind more than 16000 approximately, and China estimates to reach more than 2000 kinds.Clinical application at present less than 20 kinds, wherein named and 10 kinds of ants of being used as medicine commonly used are: band tooth ponerine, bothroponera rufipes Jerdon, Japanese hunchbacked ant, Huang are plunderred ant, Black Hills ant, red woods ant, bitooth multi-ant, Polyhachis vicina Roger (claiming Formica fusca again), phidologiton affinis Jerdon and two grand bone Pavement Ants.The ant kind that can be used as healthy food material that formal acquisition state food drugs administration approved is used as new resource food, new resources Chinese medicine is Polyhachis vicina Roger, bitooth multi-ant, blackwing subterranean, yellow-wing subterranean termite, Taiwan formosanes, and general said ant refers to Polyhachis vicina Roger more.Modern medicine is analyzed the nutritive value of ant and is identified, confirm that ant not only has very high edibleness and pharmaceutical use, and potentiality to be exploited is wide.Find through secular scientific observation, ant can be moved the object that surpasses 400 times of own body weight, secret be containing in its body the Triphosaden (ATP), cursive script formaldehyde, zinc, the heart that are described as " biological nuclear energy " receive element, formic acid, polysaccharide body, interleukin-1 and at present in the world the edibility animal fibre of unique cation be described as the 6th vital principle chitin etc. one by one beyond the protein that continues, sugar, fat, VITAMIN, the mineral substance.Ant body internal protein content is up to 42%～67%, be 4 times of egg, 6 times of milk, contain 8 seed amino acids of 28 kinds of total free aminoacidss comprising needed by human, be rich in 20 various trace elements such as several physiological active substances such as A, B, C, E vitamin and zinc, selenium, iron, magnesium, manganese, iodine, can be rated as " microfauna nutrition library " and " natural drug source mill ", the content that especially is described as " Promethean fire flower " is up to the zinc element of 200mg/kg.
China before more than 3,000 years " all gift heavenly officials " and " in the Book of Rites then " are just on the books: " fish or meat paste (being ant sauce) is presented food for the emperor ", the back Tang, bright, clear each all be documented for book on Chinese herbal medicine.With the at large first-elected great pharmacy of record man, physician's LI Shi-Zhen, according to the Compendium of Material Medica record, " ant is released name " note has " ant has the monarch and his subjects' justice, so word is from justice " for ant repellent.Again cloud " ant has it everywhere, have little, black, white, yellow, red several, the oviparity of living in caves, its residence has etc., its row has team, can know that rain waits, the spring goes out hibernation, Tu Chengfeng says ant hill ".LI Shi-Zhen has also been done penetrating argumentation to toxicity, the property of medicine, the pharmaceutical use of ant, accomplishes something difficult and so deserve praise.Modern " Chinese medicine voluminous dictionary " (version in 1986, Shanghai science tech publishing house) also included the record that Formica fusca is used as medicine.Theory of traditional Chinese medical science thinks that ant distinguishes the flavor of acid, and property is flat, returns the liver kidney channel, strengthens the body resistance to consolidate the constitution the kidney tonifying dispelling cold, the flourish muscle of nourishing the liver, the effect of eliminating blood stasis and smoothing collaterals, wherein the effect with kidney tonifying is particularly remarkable, cures mainly the dizzy tinnitus of suffering from a deficiency of the kidney, insomnia and dreamful sleep, impotence and seminal emission, rheumatic arthralgia, stroke hemiplegia, numb in every limb etc.Confirmed that the Polyhachis vicina Roger extract has outside the obvious curative effects multiple diseases such as rheumatoid arthritis, rheumatic arthritis, hepatitis B, neurasthenia, also had following main medicinal and nourishing function is as follows:
1, kidney invigorating and YANG supporting.Ant has androgen-like action, can promote the sexual organ development, and the kidney invigorating and YANG supporting effect is remarkable, and normal and other Chinese prescriptions are used for diseases such as sexual disorder, impotence, premature ejaculation, male sterility, and effect is remarkable.Modern pharmacological research finds that every 100g breeding dry ant powder contains protein 40～70g, contains the multiple amino acids and the trace element of needed by human, and wherein the content of trace element zinc is especially high, is the basic substance that improves sperm quality.
2, type ii diabetes.Clinically treat type ii diabetes with ant, effect is better, and type i diabetes is also had the assisting therapy effect.The biologist discovers, ant has extremely strong vitality, be one " microfauna nutrition treasure-house " and " natural drug source mill ", trace elements such as the necessary organic chromium of repairing islet cells, organic zinc, organic cobalt, organoselenium, organic germanium wherein particularly, be to make the islet cells reparation and the requisite element of regenerating,, participate in body carbohydrate metabolism process such as chromium, can make diabetic supersession recover normal, so can prevent and treat diabetes; Zinc plays an important role in the process that Regular Insulin produces for another example, and zinc can stimulate proinsulin to change Regular Insulin into simultaneously.China 62 tame hospitals, through 80,000 multiple-case clinical verifications, efficient to I, type ii diabetes up to more than 98%, ant can excite the beta Cell of islet function effectively, promote the generation of insulin activity and inhibition insulin antibody, both, contained the necessary trace element of repairing islet cells again for treating diabetes provides important basic substance.
3, senile dementia, old white hair, bald.Ant and the same usefulness of invigorating kidney, promoting blood circulation medicine have good curative effect to senile dementia; Can make the elderly's white hair become black sending out, hair has gloss and sturdy, and alopecia director in the crown goes out deceive and sends out, and is to eliminate old white hair, one of best protective foods of change person in middle and old age hair color.
4, osteoporosis.The latest modern technological pharmacological research shows that ant has has obvious curative effects to hypertrophic teoarthropathy pathology, osteoporosis, and name side's " JINGANG WAN " of Han dynasty treatment muscles and bones weakness adds honey refining ball with the ant abrasive dust exactly and forms.
5, antifatigue, strengthening immunity delays senility.Fatigue is the inducement of present numerous disease.According to World Health Organization's investigation, the population in the world 1/3 is in fatigue state, and about 60% middle-aged people is in fatigue state.Can synthesize a large amount of Triphosadens (ATP) in the ant body, the energy antifatigue, strengthening immunity has the effect of remarkable removing free radical, can delay senility, and prolongs organism life-span.
China utilizes the series health-care products and the medicine of ant exploitation and production that following a few class is arranged at present:
1, ant dry product.With the ant is that raw material production becomes, as the pure powder of ant, ant capsule etc.
2, ant tonic wine.With ant and glutinous rice is that the fermenting raw materials brew forms or select for use ant and multiple famous and precious animal-plant material, colludes forming through the liquor infuse, becomes Ant Queen Wine etc. as Chinese Ant Queen Wine, universe ant wine, imperial palace ant wine, will.
3, ant oral liquid class.With the ant is main raw material, adopts modern biotechnology to make with extra care and forms, as god of unusual strength's oral liquid, ant queen jelly oral liquid, ant emperor oral liquid, the profound coltfoal oral liquid of compound etc.
4, ant teas.With the ant is raw material, is equipped with date, the bark of eucommia etc. and is processed into teabag, as Du coltfoal health tea.
5, ant medicine.With the ant is main ingredient, is equipped with other plurality of Chinese and makes, as compound ant liver ball, ant-made drug for curing B-hepatitis, the spirit of ant similar rheumatism, the profound coltfoal capsule of compound, profound seven capsule for treating arthralgia aggravated by cold etc.
From as can be known above, at present commercially available ant processed goods major part be that the ant made from traditional method roughing is dried, products such as Formica fusca powder or the oral liquid of being processed into modern crafts such as water extraction, water extraction and alcohol precipitation method, alcohol extracting methods, capsule, honeyed bolus, vina, all exist the bioactive ingredients extraction efficiency not high, contain problems such as supersensitivity albumen, mouthfeel is poor, dose is big.Contain rich in protein in the ant, because people's individual difference, proteinic digestibility of ant and absorption rate are had nothing in common with each other, the ant high molecular weight protein enter behind the human body major part also do not have enough time to be absorbed promptly be excluded external, exist indigestibility, defective that specific absorption is low, problems such as supersensitivity and digestive tract reaction are particularly arranged for the part crowd, have a strong impact on its effect.
Biologically active peptides is that a class has positive influence to the bodily fuctions, finally influences the specific protein fragment of HUMAN HEALTH.Bioactive peptide is meant and derives from plant or animal-derived food product, applies to functional food and medicine and human body is had a class peptide that strengthens the human body defense function, regulates effects such as physiological rhythm, preventing disease and promotion rehabilitation.A large amount of studies show that small-molecular peptides has higher biological activity and nutritive value than amino acid, and small-molecular peptides can all absorb and utilize by enteron aisle, directly is transported to each organ of whole body and tissue and brings into play bioactive functions through the blood circulation.
Polypeptide is compared protein, amino acid whose advantage is as follows:
1, polypeptide in human intestinal absorption rate and specific absorption than amino acid height;
2, most of important hormone and organizing all is made of peptide in the human body;
3, polypeptide is to blood pressure regulation, stomachial secretion, trophic nerve, promote sleep, and regulating immunizing power has important meaning;
4, polypeptide has the function that the controlling machine bulk-growth is grown reproduction, reducing cholesterol, the absorption of promotion mineral substance in human body.
So ant protein is prepared into the small-molecular peptides that possesses the biologically active function through enzymolysis, improves healthy nutritive value and the pharmaceutical use of ant.Patent " ant ginseng oral liquid and preparation method thereof " (CN1136931) has been introduced a kind of proteolytic enzyme that uses with water is carried, protein in the ant residue after the alcohol extracting is decomposed into amino acid, polypeptide ant protein enzymatic hydrolysis process, but its selection to enzyme, enzyme hydrolysis condition etc. are not all done substantial research, owing to occur with the compound form, be unfavorable for that ant polypeptide is applied to suitability for industrialized production as independent product form.
Summary of the invention
Purpose of the present invention is in order to address the above problem, a kind of Preparation method and use of ant polypeptide is provided, the present invention utilizes the protease hydrolysis technology that ant protein is prepared into easy digestion, ant polypeptide that specific absorption is high, brings into play the proteinic medicinal and health care of ant better and is worth.Enzymatic protein is under gentleness or gentle relatively condition, comes the hydrolysis natural protein by kind, enzyme hydrolysis condition and the control reaction process of selecting enzyme, obtains specific polypeptide or bioactive peptide.
Technical scheme of the present invention is:
The preparation method of ant polypeptide is characterized in that carrying out according to the following steps:
(1), with Insecta Hymenoptera Formicidae, propagate artificially or the wild medicinal ant of edible is cleaned, be 40～60 ℃ of oven dry 6～8h in temperature, crushing screening is made dry ant powder;
(2), dry ant powder is placed CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 10～20L/h, extracting pressure is 10～40MPa, temperature is under 30～60 ℃ the condition, to carry out 1～3 hour continuous extraction, separated and collected ant oil and ant protein;
(3), with the ant protein after the degreasing, be put into enzymatic vessel, carry out enzymolysis with enzyme, enzymatic hydrolysis condition is 40～60 ℃ of temperature, pH value 6.0～7.0, and the enzyme amount that every gram ant adds is 3000～6000u, enzymolysis time is 2～8h, concentration of substrate 3%～10% obtains enzymolysis solution, and described concentration of substrate is the weight ratio of ant and water;
(4), 100 ℃ of enzymolysis solutions are carried out enzyme-deactivating, sterilization, cooled and filtered or centrifugal, obtain clarifying enzymolysis solution, carry out vacuum concentration again, lyophilize or spraying drying powder-forming make the ant polypeptide powder.
The preparation method of above-mentioned ant polypeptide, described ant is blackwing subterranean, yellow-wing subterranean termite, Taiwan formosanes, bitooth multi-ant and/or Polyhachis vicina Roger.
The preparation method of above-mentioned ant polypeptide, described dry ant powder, water content is in 10%, and granularity is 40～80 orders, as the raw material of supercritical extraction.
The preparation method of above-mentioned ant polypeptide, the extraction solvent of described supercritical extraction is that mass percent concentration is 99.5～99.9% food grade CO
2
The preparation method of above-mentioned ant polypeptide, the mass percent concentration of selecting for use of regulating pH value in the described step (3) is 10%～30% aqua calcis.
The preparation method of above-mentioned ant polypeptide, described enzyme is neutral protease, papoid and/or bromeline.
The ant polypeptide of preparation method's preparation of above-mentioned ant polypeptide is used to prepare the purposes of medicine, healthcare products, nutritious prod, food raw material or foodstuff additive.
The present invention utilizes protease hydrolysis technology enzymolysis ant protein to make the ant polypeptide powder, not only kept the original biologically active substance of ant, than protein, amino acid is easier is absorbed by the body, the ant that has overcome present utilization traditional diamond-making technique preparation does, ubiquitously in Formica fusca powder, ant health food and the medicine contain supersensitivity albumen, mouthfeel is poor, dose is big, part crowd has supersensitivity and the problem of digestive tract reaction.Because the ant polypeptide of the present invention's preparation has good solubility, stability and processing characteristics, so it is added in oral liquid, health promoting wine and the medicinal liquor, can improve the clarity of oral liquid, health promoting wine and medicinal liquor greatly, nourishing function is of the present invention one big characteristic to strengthen antifatigue, the immunomodulatory of oral liquid, health promoting wine and medicinal liquor and delay senility etc.So ant polypeptide can be widely used in medicine, healthcare products, nutritious prod, food raw material or foodstuff additive, since low, the less investment of cost, instant effect, added value height, suitability for industrialized production.
Embodiment
Embodiment 1 single enzymolysis method
500 gram ants are cleaned, and are 40 ℃ of oven dry 8h in temperature, and water content is less than 10%, and crushing screening is made 40 purpose dry ant powders, and then dry ant powder being placed mass percent concentration is 99.5% food grade CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 20L/h, extracting pressure is 30Pa, temperature is under 45 ℃ the condition, to carry out 1 hour extraction, separated and collected ant oil and ant protein; With the ant protein after the degreasing, pour enzymatic vessel into, when the enzyme amount that every gram ant adds is the neutral protease enzymolysis ant protein of 4000u, enzymatic hydrolysis condition is 50 ℃ of temperature, with mass percent concentration be 10% aqua calcis to regulate the pH value be 6, enzymolysis time 3h, concentration of substrate 3%; Enzymolysis fully after, 100 ℃ of enzyme 20min that go out, sterilization, cooling, centrifugal or filter, the filtrate vacuum concentration, concentrated solution carries out lyophilize or spraying drying, pulverizes and makes the ant polypeptide powder.
Embodiment 2 single enzymolysis methods
500 gram ants are cleaned, and are 60 ℃ of oven dry 6h in temperature, and water content is less than 10%, and grinding and sieving is made 80 purpose dry ant powders, and then dry ant powder being placed concentration is 99.9% food grade CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 10L/h, extracting pressure is 35Pa, temperature is under 50 ℃ the condition, to carry out 2 hours extraction, separated and collected ant oil and ant protein; With the ant protein after the degreasing, pour enzymatic vessel into, when the enzyme amount that every gram ant adds is the papain enzymolysis ant protein of 5000u, enzymatic hydrolysis condition is 55 ℃ of temperature, with mass percent concentration be 30% aqua calcis to regulate the pH value be 7, enzymolysis time 7h, concentration of substrate 7.5%; Enzymolysis fully after, 100 ℃ of enzyme 20min that go out, sterilization, cooling, centrifugal or filter, the filtrate vacuum concentration, concentrated solution carries out lyophilize or spraying drying, pulverizes and makes the ant polypeptide powder.
Embodiment 3 single enzymolysis methods
1000 gram ants are cleaned, and are 50 ℃ of oven dry 6h in temperature, and water content is less than 10%, and grinding and sieving is made 60 purpose dry ant powders, and then dry ant powder being placed concentration is 99.8% food grade CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 15L/h, extracting pressure is 10Pa, temperature is under 40 ℃ the condition, to carry out 3 hours extraction, separated and collected ant oil and ant protein; With the ant protein after the degreasing, pour enzymatic vessel into, when the enzyme amount that every gram ant adds is 4000u bromeline enzymolysis ant protein, enzymatic hydrolysis condition is 40 ℃ of temperature, with mass percent concentration be 20% aqua calcis to regulate the pH value be 6.5, enzymolysis time 3.5h, concentration of substrate 7%; Enzymolysis fully after, 100 ℃ of enzyme 20mi n that go out, sterilization, cooling, centrifugal or filter, the filtrate vacuum concentration, concentrated solution carries out lyophilize or spraying drying, pulverizes and makes the ant polypeptide powder.
Embodiment 4 dual-enzyme hydrolysis methods
500 gram ants are cleaned, and are 55 ℃ of oven dry 7h in temperature, and water content is less than 10%, and grinding and sieving is made 50 purpose dry ant powders, and then dry ant powder being placed concentration is 99.6% food grade CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 15L/h, extracting pressure is 40Pa, temperature is under 30 ℃ the condition, to carry out 1.5 hours extraction, separated and collected ant oil and ant protein; With the ant protein after the degreasing, pour enzymatic vessel into, when neutral protease that every enzyme amount that restrains the ant adding is 3000u and 4000u papain enzymolysis ant protein, enzymatic hydrolysis condition is 60 ℃ of temperature, with mass percent concentration is that to regulate the pH value be 6.5 for 15% aqua calcis, enzymolysis time 8h, concentration of substrate 10%; Enzymolysis fully after, 100 ℃ of enzyme 20min that go out, sterilization, cooling, centrifugal or filter, the filtrate cryogenic vacuum concentrates, concentrated solution carries out lyophilize or spraying drying, pulverizes and makes the ant polypeptide powder.
Embodiment 5 dual-enzyme hydrolysis methods
1000 gram ants are cleaned, and are 45 ℃ of oven dry 8h in temperature, and water content is less than 10%, and grinding and sieving is made 70 purpose dry ant powders, and then dry ant powder being placed concentration is 99.7% food grade CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 20L/h, extracting pressure is 25Pa, temperature is under 50 ℃ the condition, to carry out 3 hours extraction, separated and collected ant oil and ant protein; With the ant protein after the degreasing, pour enzymatic vessel into, when neutral protease that every enzyme amount that restrains the ant adding is 3000u and 5000u bromeline enzymolysis ant protein, enzymatic hydrolysis condition is 55 ℃ of temperature, with mass percent concentration is that 20% aqua calcis is regulated pH value 7, enzymolysis time 5h, concentration of substrate 6%; Enzymolysis fully after, 100 ℃ of enzyme 20min that go out, sterilization, cooling, centrifugal or filter, the filtrate cryogenic vacuum concentrates, concentrated solution carries out lyophilize or spraying drying, pulverizes and makes the ant polypeptide powder.
Embodiment 6 three enzyme hydrolysis methods
1000 gram ants are cleaned, and are 45 ℃ of oven dry 7h in temperature, and water content is less than 10%, and grinding and sieving is made 70 purpose dry ant powders, and then dry ant powder being placed concentration is 99.7% food grade CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 20L/h, extracting pressure is 25Pa, temperature is under 45 ℃ the condition, to carry out 2 hours extraction, separated and collected ant oil and ant protein; With the ant protein after the degreasing, pour enzymatic vessel into, when the enzyme amount that every gram ant adds is the papoid, 6000u neutral protease of 3000u and 6000u bromeline enzymolysis ant protein, enzymatic hydrolysis condition is 55 ℃ of temperature, with mass percent concentration is that 20% aqua calcis is regulated pH value 7, enzymolysis time 5h, concentration of substrate 6%; Enzymolysis fully after, 100 ℃ of enzyme 20min that go out, sterilization, cooling, centrifugal or filter, the filtrate cryogenic vacuum concentrates, concentrated solution carries out lyophilize or spraying drying, pulverizes and makes the ant polypeptide powder.
Claims (7)
1. the preparation method of ant polypeptide is characterized in that carrying out according to the following steps:
(1), with Insecta Hymenoptera Formicidae, propagate artificially or the wild medicinal ant of edible is cleaned, be 40～60 ℃ of oven dry 6～8h in temperature, crushing screening is made dry ant powder;
(2), dry ant powder is placed CO
2In the supercritical extraction device, regulate extraction solvent CO
2Flow velocity be 10～20L/h, extracting pressure is 10～40MPa, temperature is under 30～60 ℃ the condition, to carry out 1～3 hour continuous extraction, separated and collected ant oil and ant protein;
(3), with the ant protein after the degreasing, be put into enzymatic vessel, carry out enzymolysis with enzyme, enzymatic hydrolysis condition is 40～60 ℃ of temperature, pH value 6.0～7.0, and the enzyme amount that every gram ant adds is 3000～6000u, enzymolysis time is 2～8h, concentration of substrate 3%～10% obtains enzymolysis solution, and described concentration of substrate is the weight ratio of ant and water;
(4), 100 ℃ of enzymolysis solutions are carried out enzyme-deactivating, sterilization, cooled and filtered or centrifugal, obtain clarifying enzymolysis solution, carry out vacuum concentration again, lyophilize or spraying drying powder-forming make the ant polypeptide powder.
2. the preparation method of ant polypeptide according to claim 1, it is characterized in that: described ant is blackwing subterranean, yellow-wing subterranean termite, Taiwan formosanes, bitooth multi-ant and/or Polyhachis vicina Roger.
3. the preparation method of ant polypeptide according to claim 1, it is characterized in that: described dry ant powder, water content are in 10%, and granularity is 40～80 orders, as the raw material of supercritical extraction.
4. the preparation method of ant polypeptide according to claim 1, it is characterized in that: the extraction solvent of described supercritical extraction is that mass percent concentration is 99.5～99.9% food grade CO
2
5. the preparation method of ant polypeptide according to claim 1 is characterized in that: the mass percent concentration of selecting for use of regulating pH value in the described step (3) is 10%～30% aqua calcis.
6. the preparation method of ant polypeptide according to claim 1, it is characterized in that: described enzyme is neutral protease, papoid and/or bromeline.
7. be used to prepare the purposes of medicine, healthcare products, nutritious prod, food raw material or foodstuff additive according to the ant polypeptide of the preparation method of the described ant polypeptide of one of claim 1～6 preparation.
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Cited By (14)
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| CN102827264A (en) * | 2012-08-31 | 2012-12-19 | 哈尔滨工业大学 | Technology for separating ant maggot protein |
| CN102972727A (en) * | 2012-12-25 | 2013-03-20 | 广东省农业科学院蚕业与农产品加工研究所 | Insect-derived flavor-enhancing peptide and preparation method thereof |
| CN103880916A (en) * | 2012-12-20 | 2014-06-25 | 许世镗 | Neutral ant protein and its extraction method |
| CN103919132A (en) * | 2014-04-24 | 2014-07-16 | 邱银平 | Preparation method of ant powder containing ant polypeptides and chitosan |
| CN105614886A (en) * | 2016-01-20 | 2016-06-01 | 李蒙 | Health food for treating rheumatism |
| CN105753934A (en) * | 2015-06-16 | 2016-07-13 | 刘公德 | Antifreeze protein fluid extracting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102827264A (en) * | 2012-08-31 | 2012-12-19 | 哈尔滨工业大学 | Technology for separating ant maggot protein |
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