CN104687051A - Marine organism nutrient solution and preparation method thereof - Google Patents
Marine organism nutrient solution and preparation method thereof Download PDFInfo
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- CN104687051A CN104687051A CN201510124493.4A CN201510124493A CN104687051A CN 104687051 A CN104687051 A CN 104687051A CN 201510124493 A CN201510124493 A CN 201510124493A CN 104687051 A CN104687051 A CN 104687051A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a marine organism nutrient solution and a preparation method thereof. The marine organism nutrient solution is formed by extracting and matching fresh oysters, prawns and sea otters that are originated from the Beibu Gulf. The preparation method of the marine organism nutrient solution uses papain enzymolysis, saccharomycetes fermentation in combination with active carbon for removing the fishy smell and decoloring, thereby solving the problem of the specific fishy smell of the marine organism products. A browning inhibitor and a composite stabilizer are added for solving the problems of the severe browning and sediment that are caused in the processing (enzymolysis, sterilization and storage) of the marine organism nutrient solution. The oysters, the prawns and the sea otters are sea products high in protein and low in fat; the amino acid composition is complete, wherein the complete degree of the necessary amino acid and the mass proportion are better than cow milks and human milks. The compatibility of the oyster and prawn extracting solutions as well as the sea otter extracting solution has physiological functions of invigorating the kidney and strengthening Yang, strengthening the organic immunity, protecting the liver, regulating the blood sugar level, reducing the blood fat, relieving the fatigue, reducing the blood pressure, and the like. The marine organism nutrient solution can be made into a series of food that has a unique flavor, a light yellow color and a delicious taste, wherein the series of food has multiple physiological health care functions.
Description
Technical field
The present invention relates to a kind of marine organism health food and preparation method thereof, is ocean biology nutrition liquid prepared for raw material with large oyster, prawn, sea otter and preparation method thereof specifically.
Background technology
Large oyster, also known as Crassostrea rivularis, is a kind of common seashells animal, belongs to Mollusca, lamellibranchiata (Lamellibranchia), Ostreidae (Ostreidae), and oyster belongs to.Large oyster is Fresh & Tender in Texture, is a kind of nourishing food of high nutritive value, has the title of " seabed milk ".Research shows, the trace element containing abundant glycogen, protein, amino acid, needed by human in oyster meat, vitamin and the more unrighted acid with important physiologically active.Compendium of Material Medica is recorded: " raw oyster, controls deficient, establishing-Yang, removing toxic substances, mends men and women's qi and blood, makes skin delicate, health care labor ".Raw oyster oyster extract can treat various diseases, and main component glycogen can supplement rapidly and regain one's strength, and can improve body immunity; Taurine it can be good at reducing blood lipid, hypotensive; Zn content is the highest in wholefood, effectively can strengthen male's sexual; Contained multivitamin and mineral matter particularly selenium can regulate nerve, set the mind at rest; Abundant is calcareous, and skin can be made lubricious; Potassium can treat dry skin and acne.
Prawn, for crust subphylum (Crustacea) Decapoda (Decapoda) is swum suborder (Natantia) animal.The benefiting action of prawn and medical value are all higher.According to analysis, moisture content 77 grams in the fresh shrimp of every hectogram, 20.6 grams, protein, 0.7 gram, fat, calcium 35 milligrams, 150 milligrams, phosphorus, iron 0.1 milligram, 3-Hydroxyretinol 60 international unit, also containing vitamin B1, vitamin B2, vitamin E, niacin etc.Motherland's medical science is thought, shrimp taste is sweet, salty, warm in nature, the merit having establishing-Yang kidney-nourishing, mend essence, lead to breast, and every weakness due to chronic disease, shortness of breath and fatigue, diet are not thought, the yellow thin thin people in face, all can using it as nourishing and remedy diet.
Sea otter (Syngnathus), be Syngnathidae animal, sea otter is a parts of generic medicinal plants, sweet, salty, warm.Return liver, kidney channel, there is the curative effects such as warm kidney benefit essence, promoting blood circulation to remove blood stasis, swelling and pain relieving.Containing rich in protein, peptide class, amino acid, fat, epidermis secretion mucopolysaccharide, 4-cholestenone, cholesterine, N-PBNA, myristic acid, palmitic acid, stearic acid etc. in sea otter.
Large oyster, prawn, sea otter contain special acid and various trace elements because of it, not only have very high edibility, and have unique medicinal health function.
Over nearly 10 years, make a general survey of domestic and international commercialized product, domestic also have some large oyster goods to emerge, as: " extra large king's OYSTER EXTRACT CAPSULE " that Hai Wang group produces, the donkey-hide gelatin oyster oral liquid of Xinjiang Shi Dan pharmaceutcal corporation, Ltd development, the oyster Qi blade of Yuan Chen bio tech ltd, Wuhan, the selenium OYSTER EXTRACT CAPSULE zinc metal sheet of Guang Ju bio tech ltd, Guangzhou, the ecological sheet of the oyster that Nanning Yi Zhen biological products Co., Ltd produces etc., but be all for raw material with single oyster extract, also unexposed explanation is effectively removed the marine organisms goods peculiar fishy smell of institute and how effectively to be prevented protein food to be long placed in brown stain, the problems such as precipitation.In addition the developed country such as American-European, Japanese is also at marine organisms and functional active components thereof such as research and development oysters, but is more also in laboratory stage, not yet gives full play to social economic value.Be directed to marine product the problems referred to above, conventional method be utilize acid, alkali is hydrolyzed to bioprotein, due to its poor stability, product effect lose significantly, the problem such as nutrient component damages, now gradually replace by bioprotein zymotechnic.Marine product through enzyme digestion reaction, generate objective function active peptides or amino acid whose while, partial hydrolysate is polypeptide or the bitter peptides that molecular end contains hydrophobic amino acid, causes hydrolysate to present bitter taste or fishy smell, have impact on the local flavor of product.Develop the product be combined into by multiple marine organisms, and effectively can remove the peculiar fishy smell of marine organisms goods, effectively prevention is long placed in the problem such as brown stain, precipitation, will be subject to market favor, also will promote that the exploitation of marine organisms goods is to benign development.
At present, the system research to large oyster, prawn, Syngnathus extract use in conjunction had not been had.
Summary of the invention
The object of the present invention is to provide a kind of ocean biology nutrition liquid prepared for raw material with large oyster, prawn, sea otter and preparation method thereof.
A preparation method for ocean biology nutrition liquid, comprises the steps:
1) get fresh large oyster software, the prawn human body, cleaning, adds pure water, homogenate;
2) by step 1) homogenate that obtains adopts papain enzymolysis;
3) by step 2) enzymolysis liquid that obtains carries out enzyme-deactivating, adds culture propagation;
4) by step 3) zymotic fluid that obtains by activated carbon decolorizing, filter, collect filtrate;
5) to step 4) obtain in filtrate, adding browning inhibitor and compound stabilizer, mixing, centrifugally obtains large oyster, prawn extract;
6) get drying after sea otter, pulverize, mix and be placed in percolator, in cylinder, add edible ethanol, diacolation after leaving standstill, collect percolate, filter, filtrate recycling ethanol, remove the percolate after ethanol and concentrate, staticly settle, filter and obtain sea otter percolate I;
7) add pure water and enter step 6) containing the percolator of the dregs of a decoction, diacolation after leaving standstill, collects and obtains percolate II;
8) to step 7) dregs of a decoction that obtain adopt papain enzymolysis, and after enzymolysis terminates, enzyme-deactivating, filters, obtains percolate III;
9) sea otter percolate I, II, III is merged, concentrated, add distilled water low-temperature precipitation, after getting supernatant liquid filtering, obtain sea otter extract;
10) by step 5) the large oyster that obtains, prawn extract and step 9) the sea otter extract mixing that obtains, sterilizing obtains;
Described browning inhibitor is bamboo-leaves flavones 0.03-0.08% (w/v), neutral sodium phytate 0.03-0.08% (w/v);
Described compound stabilizer is xanthans 0.15-0.25% (w/v), pectin 0.25-0.35% (w/v), CMC (carboxymethyl cellulose) 0.10-0.20% (w/v).
Large oyster of the present invention, prawn, sea otter preferably originate from the North Sea.
The preferred hydrolysis temperature of described papain enzymolysis 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v).
Step 1) described in large oyster software refer to the pulp obtained after large oyster is shelled, the prawn human body refers to the pulp that prawn removes shrimp head, shrimp pin, crust obtain;
Step 1) described in large oyster software, the prawn human body preferably mix according to the weight ratio of 1:0.5-1, the 2-3 of the pure water preferred feedstock weight added is doubly.
Step 3) described in fermentation, preferred fermentation temperature 25 DEG C, fermentation time 60min, inoculum of dry yeast 1.0% (w/v).
Step 4) described in activated carbon decolorizing, preferred process temperature 35 DEG C, processing time 50min, addition 1.5% (w/v).
Step 5) described in centrifugal preferred 3000rpm, 15min.
Step 6) described in the edible ethanol of preferred 95% (v/v) of diacolation, leave standstill as 16-30h, preferred 24h, when ethanol flow is per kilogram spice 3-7 milliliter per minute, collecting weight is stop diacolation after spice gross weight 2.5-4 percolate doubly.
Step 7) described in leave standstill as 1-3h, preferred 2h, stops diacolation when collecting after weight is the percolate II of spice gross weight twice.
Step 9) described in concentrated preferably at ambient pressure control temperature be 100 DEG C and concentrate, be concentrated into when weight is 1/4 of spice gross weight and add triplication distilled water again, below 4 DEG C, cool precipitation 24 hours in cold-room.
Step 10) described in large oyster, prawn extract and sea otter extract preferably mix according to the weight ratio of 1:0.25-1.
The invention still further relates to the ocean biology nutrition liquid adopting above-mentioned preparation method to obtain, in order to obtain suitable mouthfeel, the ocean biology nutrition liquid obtained also adds auxiliary material (sorbic acid, honey, sucrose etc.) to be allocated, to mix edible denseness, taste etc., also can mix with pharmaceutical excipient or food additives, be prepared into any one formulations such as oral agents, capsule, granule, tablet, pill, electuary.
Compared to the prior art, the invention has the advantages that:
1, fresh large oyster and prawn mixing are carried out activity extraction by the present invention first, and effectively can remove large oyster, the peculiar fishy smell of prawn, and effectively prevention is long placed in brown stain, precipitation, extends the shelf life.
2, the fat-soluble height of phospholipid composition in sea otter, could propose with the edible ethanol (95%) of high concentration, and proteinaceous components has water-soluble, it is higher that water extraction obtains rate.The present invention successively extracts sea otter use water, alcohol two kinds of solvents, the sea otter dregs of a decoction after extraction just can as the good fish protein hy substrate of one, enzymolysis is carried out to the sea otter dregs of a decoction, hydrolysis amino acid and polypeptide can be obtained, to the comprehensive utilization of rare medicinal herbs sea otter, improve the yield of active ingredient.Large oyster and prawn active compound and Syngnathus extract compatibility are studied, obtain there is tonifying kidney and strengthening yang, develop immunitypty, protect liver, invigorate blood circulation, the ocean biology nutrition liquid of hypotensive Adjust-blood lipid effect.
3, the invention provides one and combine de-raw meat, prevent the processing method precipitated: adopt Papain enzymolysis and yeast fermentation method, defishying is carried out to large oyster, prawn enzymolysis liquid; Then adopt active carbon to decolour further, can effectively remove marine product fishy smell, improve enzymolysis liquid color and luster; Add browning inhibitor, optimize color and luster; Add compound stabilizer, solve the sedimentation problem that it produces in enzymolysis, sterilization and storage.
Detailed description of the invention
With embodiment, the present invention will be described below, but the present invention is not limited to these embodiments.
Embodiment 1
A preparation method for ocean biology nutrition liquid, comprises the steps:
1) get the fresh North Sea large oyster software 100g, North Sea prawn human body 50g, cleaning, adds pure water 300mL, the abundant homogenate of tissue refiner;
2) by step 1) homogenate that obtains adopts papain enzymolysis, hydrolysis temperature 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v);
3) by step 2) enzymolysis liquid that obtains, at 70 DEG C, heating carries out enzyme-deactivating in 20 minutes, adds culture propagation, fermentation temperature 25 DEG C, fermentation time 60min, inoculum of dry yeast 1.0% (w/v);
4) by step 3) zymotic fluid that obtains is by activated carbon decolorizing, and treatment temperature 35 DEG C, processing time 50min, addition 1.5% (w/v), filter, and collects filtrate;
5) to step 4) obtain in filtrate, adding browning inhibitor and compound stabilizer, mixing, centrifugal 3000rpmX15min, obtain large oyster, prawn extract, browning inhibitor is bamboo-leaves flavones 0.03% (w/v), neutral sodium phytate 0.08% (w/v), and compound stabilizer is xanthans 0.15% (w/v), pectin 0.35% (w/v), CMC 0.10% (w/v);
6) get drying after North Sea sea otter, pulverize, mix and be placed in percolator, in cylinder, add 95% (v/v) edible ethanol, leave standstill 16h, diacolation, when ethanol flow is per kilogram spice 3-7 milliliter per minute, collecting weight is stop diacolation after the percolate of spice gross weight 4 times, collect percolate, filter, filtrate recycling ethanol, remove the percolate after ethanol concentrate, staticly settle 30h, filter obtain sea otter percolate I;
7) add pure water and enter step 6) containing the percolator of the dregs of a decoction, leave standstill 1h, diacolation, stops diacolation when collecting after weight is the percolate II of spice gross weight twice, and collect and obtain percolate II;
8) to step 7) dregs of a decoction that obtain adopt papain enzymolysis, hydrolysis temperature 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v), after enzymolysis terminates, at 70 DEG C, heating carries out enzyme-deactivating in 20 minutes, filter, obtain percolate III;
9) sea otter percolate I, II, III is merged, insert in jacketed pan, boil rear slow fire control temperature under normal pressure to be 100 DEG C and to concentrate, be concentrated into when weight is 1/4 of spice gross weight and add triplication distilled water again, precipitation is cooled after 24 hours in 4 DEG C of cold-rooms, get supernatant liquor suction filtration machine and filter clarification, namely make sea otter extract;
10) by step 5) the large oyster that obtains, prawn extract and step 9) the sea otter extract that obtains mixes according to the weight ratio of 1:1, add 1% sucrose (w/w), add 1% citric acid (w/w), mix thoroughly, sterilizing obtains.
Embodiment 2
A preparation method for ocean biology nutrition liquid, comprises the steps:
1) get the fresh North Sea large oyster software 100g, North Sea prawn human body 100g, cleaning, adds pure water 600mL, the abundant homogenate of tissue refiner;
2) by step 1) homogenate that obtains adopts papain enzymolysis, hydrolysis temperature 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v);
3) by step 2) enzymolysis liquid that obtains, at 70 DEG C, heating carries out enzyme-deactivating in 20 minutes, adds culture propagation, fermentation temperature 25 DEG C, fermentation time 60min, inoculum of dry yeast 1.0% (w/v);
4) by step 3) zymotic fluid that obtains is by activated carbon decolorizing, and treatment temperature 35 DEG C, processing time 50min, addition 1.5% (w/v), filter, and collects filtrate;
5) to step 4) obtain in filtrate, adding browning inhibitor and compound stabilizer, mixing, centrifugal 3000rpmX15min, obtain large oyster, prawn extract, browning inhibitor is bamboo-leaves flavones 0.08% (w/v), neutral sodium phytate 0.03% (w/v), and compound stabilizer is xanthans 0.25% (w/v), pectin 0.25% (w/v), CMC 0.20% (w/v);
6) get drying after North Sea sea otter, pulverize, mix and be placed in percolator, in cylinder, add 95% (v/v) edible ethanol, leave standstill 30h, diacolation, when ethanol flow is per kilogram spice 3-7 milliliter per minute, collecting weight is stop diacolation after the percolate of spice gross weight 2.5 times, collect percolate, filter, filtrate recycling ethanol, remove the percolate after ethanol concentrate, staticly settle 16h, filter obtain sea otter percolate I;
7) add pure water and enter step 6) containing the percolator of the dregs of a decoction, leave standstill 3h, diacolation, stops diacolation when collecting after weight is the percolate II of spice gross weight twice, and collect and obtain percolate II;
8) to step 7) dregs of a decoction that obtain adopt papain enzymolysis, hydrolysis temperature 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v), after enzymolysis terminates, at 70 DEG C, heating carries out enzyme-deactivating in 20 minutes, filter, obtain percolate III;
9) sea otter percolate I, II, III is merged, insert in jacketed pan, boil rear slow fire control temperature under normal pressure to be 100 DEG C and to concentrate, be concentrated into when weight is 1/4 of spice gross weight and add triplication distilled water again, precipitation is cooled after 24 hours in 4 DEG C of cold-rooms, get supernatant liquor suction filtration machine and filter clarification, namely make sea otter extract;
10) by step 5) the large oyster that obtains, prawn extract and step 9) the sea otter extract that obtains mixes according to the weight ratio of 1:0.5, take 25% (w/w) white sugar, be cooked into the simple syrup of 85%, clarification is filtered with suction filtration machine after adding the mixing of 15% (w/w) formula ratio honey, again this syrup and synthesis liquid are stirred, finally add the sorbic acid accounting for spice gross weight 1.2 ‰, with distilled water, liquid proportion is adjusted to 1.29, namely simmering in water boils completes the making of product for 15 minutes.Through packing after the assay was approved, sealing, sterilizing, label, pack, put in storage.
Embodiment 3
A preparation method for ocean biology nutrition liquid, comprises the steps:
1) get the fresh North Sea large oyster software 100g, North Sea prawn human body 50g, cleaning, adds pure water 400mL, the abundant homogenate of tissue refiner;
2) by step 1) homogenate that obtains adopts papain enzymolysis, hydrolysis temperature 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v);
3) by step 2) enzymolysis liquid that obtains, at 70 DEG C, heating carries out enzyme-deactivating in 20 minutes, adds culture propagation, fermentation temperature 25 DEG C, fermentation time 60min, inoculum of dry yeast 1.0% (w/v);
4) by step 3) zymotic fluid that obtains is by activated carbon decolorizing, and treatment temperature 35 DEG C, processing time 50min, addition 1.5% (w/v), filter, and collects filtrate;
5) to step 4) obtain in filtrate, adding browning inhibitor and compound stabilizer, mixing, centrifugal 3000rpmX15min, obtain large oyster, prawn extract, browning inhibitor is bamboo-leaves flavones 0.06% (w/v), neutral sodium phytate 0.05% (w/v), and compound stabilizer is xanthans 0.20% (w/v), pectin 0.30% (w/v), CMC 0.15% (w/v);
6) get drying after North Sea sea otter, pulverize, mix and be placed in percolator, in cylinder, add 95% (v/v) edible ethanol, leave standstill 24h, diacolation, when ethanol flow is per kilogram spice 3-7 milliliter per minute, collecting weight is stop diacolation after the percolate of spice gross weight 3.0 times, collect percolate, filter, filtrate recycling ethanol, remove the percolate after ethanol concentrate, staticly settle 24h, filter obtain sea otter percolate I;
7) add pure water and enter step 6) containing the percolator of the dregs of a decoction, leave standstill 2h, diacolation, stops diacolation when collecting after weight is the percolate II of spice gross weight twice, and collect and obtain percolate II;
8) to step 7) dregs of a decoction that obtain adopt papain enzymolysis, hydrolysis temperature 40 DEG C, enzymolysis time 2h, pH7, enzyme addition 0.4% (w/v), after enzymolysis terminates, at 70 DEG C, heating carries out enzyme-deactivating in 20 minutes, filter, obtain percolate III;
9) sea otter percolate I, II, III is merged, insert in jacketed pan, boil rear slow fire control temperature under normal pressure to be 100 DEG C and to concentrate, be concentrated into when weight is 1/4 of spice gross weight and add triplication distilled water again, precipitation is cooled after 24 hours in 4 DEG C of cold-rooms, get supernatant liquor suction filtration machine and filter clarification, namely make sea otter extract;
10) by step 5) the large oyster that obtains, prawn extract and step 9) the sea otter extract that obtains mixes according to the weight ratio of 1:0.25, sterilizing obtains.
Experimental example: effect experiment in body (tonifying kidney and strengthening yang, develop immunitypty, protect liver, invigorate blood circulation, lipopenicillinase, step-down)
Effect experiment one: on the impact of Wistar rat tonifying kidney and strengthening yang
1, test drug
The ocean biology nutrition liquid made with embodiment 3.
2, animal
Wistar rat, SPF level, male, body weight 200-250g.
3, method: select body weight at the Wistar male rat 40 of 200-220g, be divided into 5 groups at random, often organize 8, i.e. normal group, kidney-yang deficiency model group, the high, medium and low dosage group of ocean biology nutrition liquid, except normal group, make castration model for other 4 groups, at the chloraldurate (0.056mlkg of 5%
-1) anaesthetizing descending bilateral orchidectomy, postoperative muscle injection penicillin 40,000 U, injection 3d is with anti-infective continuously.High, medium and low dosage respectively according to ocean biology nutrition liquid (10.0,5.0,1.0gkg
-1) gavage, normal group, kidney-yang deficiency model group only gavage the distilled water (0.1mlkg of same volume
-1).
3.1, to erect preclinical mensuration
1h after last 1 administration, is placed in rat penis position (electrode is put in orificium urethrae externum, and another electrode is placed in radix penis part), dips in the moistening rat radix penis part of physiological saline, to strengthen its electric conductivity with cotton swab by the stimulating electrode of electric stimulating instrument.Give local electrical stimulation, voltage 6V, frequency 20Hz, the wide 5ms of ripple, start to telotism time (erection incubation period) from stimulation with stopwatch record.
3.2, the mensuration of level of serum testosterone
After rat claims quality, lumbar injection 5% chloraldurate, 0.56mlkg
-1, anesthetized rat, docking gets blood about 1.5ml, 2000rapmin after blood clotting
-1, centrifugal 15min, separation of serum ,-20 DEG C of Cord blood are for subsequent use.The Full-automatic chemiluminescence immunoassay analysis meter (DXI-800) using Bei Ke Man to produce and matched reagent thereof, with automatic lmunoassays analyzer within reagent calibration cycle and Quality Control, according to instrumentation Programmable detection rat blood serum testosterone levels.
4, data processing
Experimental data represents with (X ± s), and data statistics SPSS11.0 software carries out, and between group, contrast adopts one wayanova to carry out.
Note: * * is P<0.01, * P<0.05 compared with model group compared with normal group
5, result and discussion
Castrated rats animal pattern experimental result shows, with Normal group ratio, model group rats eclipse period of penile erection significant prolongation illustrates modeling success.The each dosage group of ocean biology nutrition liquid compares with castration model group can significantly shorten castrated rats eclipse period of penile erection, improves castrated rats penis to the excitability of outside stimulus, significantly increases Ovariectomized Rat Serum testosterone levels, and have certain dose-effect relationship.
Effect experiment two: on the impact of immune function of mice
1, test drug
The ocean biology nutrition liquid made with embodiment 3.
2, animal
Kunming mouse, male, body weight 22-25 gram.
3, method
Three composition dosage groups established by medicine, separately establish blank group and marine bioactivity combination liquid group, sea otter percolate group, ocean biology nutrition liquid group.Gastric infusion, capacity is 0.2ml/10g body weight.Once a day, continuous 14 days.After last administration after 1 hour, animal tail vein injection 0.1 india ink, gets blood with glass capillary through eye rear vein beard in 1 minute and 5 minutes, each 0.02 milliliter.Blood is added and fills 2 milliliter of 0.1% sodium carbonate liquor in vitro, shake up, at spectrophotometer 680nm wavelength place recording light density (OD value).By formula K=(OD1-OD5)/(T5-T1) calculating K value, represent the phagocytic rate of mouse.
4, data processing
Experimental data represents with (X ± s), and data statistics SPSS11.0 software carries out, and between group, contrast adopts one wayanova to carry out.
* is compared, P < 0.05 with blank group; *, P < 0.01
5, result and discussion
Mouse carbonic clearance experimental result sees the following form.As seen from the table, composition can increase phagocytic rate, strengthens monocytic phagocytic function, strengthens nonspecific immunity function.
Effect experiment three: the impact of DEN being induced to SD rat liver cancer
1, test drug
The ocean biology nutrition liquid made with embodiment 3.
2, animal
SD rat, male, body weight 200-250g.
3, method
Male SD rat 90 is divided into diethylnitrosamine (diethylinitrosamine, DEN) group, Normal group at random, ocean biology nutrition liquid group.DEN group and ocean biology nutrition liquid group give 0.2%DEN solution gavage, by body weight 10mgkg
-1administration, 5 times weekly, to 14 weeks, ocean biology nutrition liquid group simultaneously gavage (ig) gave ocean biology nutrition liquid solvent, every day 1 time, until 14 weeks.Blank group and DEN model group such as to give respectively at the physiological saline of capacity.Animal is put to death after anesthesia at the end of the 16th week of experiment.Animal cuts abdominal cavity open after adopting 3% chloral hydrate anesthesia, and conventional 10% formalin of the whole liver of clip is fixed, FFPE, 4-6 μm of serial section, VG dyes, and immunohistochemical staining, light Microscopic observation is on the incidence of the rat liver cancer that DEN induces and multifarious impact.
4, data processing
Experimental data with
represent, data statistics SPSS11.0 software carries out, and between group, contrast adopts one wayanova to carry out.
Note: compare with Normal group, P**<0.01, compares with DEN group, P*<0.05.
5, result and discussion
When 16 weeks, the incidence of the Rat Hepatocellular Carcinoma of DEN induction is 100%, and diversity is (3.62 ± 2.13).Liver-protecting tablet reduces incidence (P < 0.05) and the diversity (P < 0.05) of Rat Hepatocellular Carcinoma.
Effect experiment four: on the impact of reducing blood pressure and blood fat
1, test drug
The ocean biology nutrition liquid made with embodiment 3.
2, animal
Original hypertensive rat (spontaneously hyperten-sive rat, SHR), 30,19 week age, male, (350 ± 20) g.
Method
30 SHR are divided into 5 groups at random, often organize 6, be respectively the basic, normal, high dosage group of ocean biology nutrition liquid (1,5,10gkg
-1), Captopril group (20mgkg
-1) and negative control group.Each group respectively gavage give relative medicine, negative control group gavage gives physiological saline.Long term administration is tested: every day timed drug administrations, continue 4 weeks, every 1 week measurement rat blood pressure and heart rate, record the change of body weight simultaneously.
4, data processing
Experimental data is with means standard deviation
represent, before and after administration, blood pressure adopts the single factor test t-method of inspection, adopts one-way analysis of variance to compare between experimental group, adopts SPSS software to carry out data statistic analysis.
Compare with negative control group, * P < 0.05, * * P < 0.01
5, result and discussion
Through the long term administration experiment of 4 weeks, the situation of change of SHR blood pressure was as shown in table 2.The pressure value continuing each dosage group and Captopril group giving ocean biology nutrition liquid, compared with negative control group, compared with before administration, all has significant downward trend.And between the pressure value of each dosage group measurement when the 1st, 2,3,4 week after taking, difference is remarkable, all remains on lower pressure value level.This explanation continues to take the pressure value that ocean biology nutrition liquid not only can reduce SHR, and blood pressure can remain on maintenance level.
Claims (5)
1. a preparation method for ocean biology nutrition liquid, is characterized in that, comprises the steps:
1) get fresh large oyster software, the prawn human body, cleaning, adds pure water, homogenate;
2) by step 1) homogenate that obtains adopts papain enzymolysis;
3) by step 2) enzymolysis liquid that obtains carries out enzyme-deactivating, adds culture propagation;
4) by step 3) zymotic fluid that obtains by activated carbon decolorizing, filter, collect filtrate;
5) to step 4) obtain in filtrate, adding browning inhibitor and compound stabilizer, mixing, centrifugally obtains large oyster, prawn extract;
6) get drying after sea otter, pulverize, mix and be placed in percolator, in cylinder, add edible ethanol, diacolation after leaving standstill, collect percolate, filter, filtrate recycling ethanol, remove the percolate after ethanol and concentrate, staticly settle, filter and obtain sea otter percolate I;
7) add pure water and enter step 6) containing the percolator of the dregs of a decoction, diacolation after leaving standstill, collects and obtains percolate II;
8) to step 7) dregs of a decoction that obtain adopt papain enzymolysis, and after enzymolysis terminates, enzyme-deactivating, filters, obtains percolate III;
9) sea otter percolate I, II, III is merged, concentrated, add distilled water low-temperature precipitation, after getting supernatant liquid filtering, obtain sea otter extract;
10) by step 5) the large oyster that obtains, prawn extract and step 9) the sea otter extract mixing that obtains, sterilizing obtains;
Described browning inhibitor is bamboo-leaves flavones 0.03-0.08%, neutral sodium phytate 0.03-0.08%;
Described compound stabilizer is xanthans 0.15-0.25%, pectin 0.25-0.35%, CMC0.10-0.20%.
2. the preparation method of a kind of ocean biology nutrition liquid according to claim 1, is characterized in that: described large oyster, prawn, sea otter originate from the North Sea.
3. the preparation method of a kind of ocean biology nutrition liquid according to claim 1, is characterized in that: step 1) described in large oyster software, the prawn human body mix according to the weight ratio of 1:0.5-1, the pure water added be the 2-3 of raw material weight doubly.
4. the preparation method of a kind of ocean biology nutrition liquid according to claim 1, is characterized in that: step 10) described in large oyster, prawn extract and sea otter extract mix according to the weight ratio of 1:0.25-1.
5. the ocean biology nutrition liquid that obtains of method according to any one of claim 1-4.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104887713A (en) * | 2015-06-23 | 2015-09-09 | 广西北部湾海皇生物科技有限公司 | Preparing method of oyster active extract having functions of producing sperm to tonify the kidney and improving sexual function |
| CN108244444A (en) * | 2018-02-08 | 2018-07-06 | 舟山海研食品科技有限公司 | Oyster Protein small-molecular peptides solid beverage and preparation method thereof |
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| CN1126615A (en) * | 1995-09-20 | 1996-07-17 | 王成飞 | Allopelagic health oral liquid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104887713A (en) * | 2015-06-23 | 2015-09-09 | 广西北部湾海皇生物科技有限公司 | Preparing method of oyster active extract having functions of producing sperm to tonify the kidney and improving sexual function |
| CN108244444A (en) * | 2018-02-08 | 2018-07-06 | 舟山海研食品科技有限公司 | Oyster Protein small-molecular peptides solid beverage and preparation method thereof |
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