CN103054113B - Composite fruit-vegetable juice beverage with blood sugar decreasing effect - Google Patents
Composite fruit-vegetable juice beverage with blood sugar decreasing effect Download PDFInfo
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- CN103054113B CN103054113B CN201310030088.7A CN201310030088A CN103054113B CN 103054113 B CN103054113 B CN 103054113B CN 201310030088 A CN201310030088 A CN 201310030088A CN 103054113 B CN103054113 B CN 103054113B
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Abstract
The invention discloses a composite fruit-vegetable juice beverage with a blood sugar decreasing effect. The composite fruit-vegetable juice beverage comprises the following raw materials in parts by weight: 10-30 parts of cactus juice, 10-20 parts of kakorot juice, 5-15 parts of balsam pear juice, 5-15 parts of pumpkin juice, 10-20 parts of aloe-guava mixed juice, 4-8 parts of xylitol, 0.4-0.6 part of honey and 0.1-0.3 part of a compound stabilizer. The composite fruit-vegetable juice beverage disclosed by the invention is uniform in color and luster, has special natural faint scent of composite fruit-vegetable juice and is sour, sweet and tasty, the appearance is free of obvious sediment, the juice is not layered, the liquidity is good, and effective constituents of the different raw materials are adequately mixed and complemented with one another; and the composite fruit-vegetable juice beverage meets the national fruit-vegetable juice beverage sanitary standard GB19297-2003, not only can satisfy sour, sweet and tasty mouth feels of the composite fruit-vegetable juice, but also has a blood sugar decreasing function.
Description
One, technical field
The present invention relates to a kind of functional beverage, be specifically related to a kind of compound fruit and vegetable juice beverage with effect of lowering blood sugar.
Two, background technology
In recent years, quickening along with growth in the living standard and rhythm of life, modern's diet is unbalanced, and diet structure is unreasonable, and takes in a large number high confectionery thing, cause human homergy to occur variety of issue, high-carbonhydrate diet can change blood pH, reduces immunity of organisms, affects body fat consumption, cause fat accumulation, finally cause the various diseases in various degree such as atherosclerotic, diabetes, cardiovascular and cerebrovascular diseases, coronary heart disease and microangiopathies.
Although beverage types is in the market various, great majority are only focused on mouthfeel and functional deficiency is especially less for the drinks of the special populations such as diabetes patient.In addition, the raw material ratio of components of beverage is more single, not only some beverage can not improve the health status of sub-health population, and long-term drinking also may have side effects to human body.
Three, summary of the invention
The present invention aims to provide a kind of compound fruit and vegetable juice beverage with effect of lowering blood sugar, compound fruit and vegetable juice beverage of the present invention meets national fruit-vegetable juice beverage sanitary standard GB19297-2003, can meet the sour-sweet salubrious mouthfeel of compound fruit and vegetable juice beverage, can reach again hypoglycemic health care object, and without any side effects.
The compound fruit and vegetable juice beverage that the present invention has effect of lowering blood sugar is formulated by Cactus juice, leprosy grape juice, Bitter Melon Juice, Pumpkin Juice, asparagus juice and guava juice and other auxiliary materials.
The raw material that the present invention has the compound fruit and vegetable juice beverage of effect of lowering blood sugar is configured to by mass fraction:
Cactus juice 10-30 part, leprosy grape juice 10-20 part, Bitter Melon Juice 5-15 part, Pumpkin Juice 5-15 part, aloe and guava mixed juice 10-20 part, xylitol 4-8 part, honey 0.4-0.6 part, compound stabilizer 0.1-0.3 part.
Described compound stabilizer is to be mixed and form by the mass ratio of 1:1:2 by sodium alginate, pectin and sodium carboxymethylcellulose (CMC-Na).
Described Cactus juice is that fresh prickly pear by deburring and after cleaning (without going rotten, without disease and pest, have no mechanical damage) is mixed according to the ratio of mass ratio 1:10 with water, after making beating at 65-75 ℃ lixiviate 10-15min, cross the Cactus juice obtaining after 200 order filter clothes.
Described leprosy grape juice is the leprosy grape juice obtaining after pretreatment, crushing and beating and the enzyme that goes out;
Described pretreatment be will go seed and clean after fresh leprosy grape be cut into the sheet piece that 0.4-0.6cm is thick, add blanching 5-8 minute in the water of 85-95 ℃ to obtain pretreated leprosy grape;
Described crushing and beating is that pretreated leprosy grape is cooled to normal temperature, the citric acid colour protecting liquid (being aqueous citric acid solution) that adds the mass concentration 0.2% of 3 times of quality, the pectase that adds broken leprosy grape quality 0.1% after fragmentation, standing 2h under 35 ℃, pH5.5, obtains leprosy grape slurry after making beating;
The described enzyme that goes out is that described leprosy grape is starched in 100 ℃ of heating 20min enzymes that go out, then uses 200 order filter-cloth filterings, removes filter residue and obtains leprosy grape juice.
Described Bitter Melon Juice is through pretreatment, removes the Bitter Melon Juice that obtains after bitter taste, crushing and beating and the enzyme that goes out;
Described pretreatment be will go the fresh balsam pear of flesh after cleaning be cut into the sheet piece that 0.4-0.6cm is thick, drop into hot water blanching 5-8 minute of 85-95 ℃ to protect look, obtain pretreated balsam pear;
The described bitter taste that goes is that pretreated balsam pear is placed in to mass concentration is that 10% NaCl solution soaks 30-50min, takes out and is placed in boiling water rinsing 2-3 time, utilizes the bitter principle effect removal bitter taste in salt and balsam pear;
Described crushing and beating is by the balsam pear going after bitter taste, is cooled to normal temperature, adds the pectase of broken balsam pear quality 0.1% after fragmentation, and standing 2h under 35 ℃, pH5.5 obtains balsam pear slurry after making beating;
The described enzyme that goes out is that above-mentioned balsam pear is starched in 100 ℃ of heating 20min enzymes that go out, then uses 200 order filter-cloth filterings, removes filter residue and obtains Bitter Melon Juice.
Described Pumpkin Juice is the Pumpkin Juice obtaining after pretreatment, softening making beating, enzymolysis and the enzyme that goes out;
Described pretreatment is to go the pumpkin after seed flesh is also cleaned to be cut into the sheet piece that 4-6mm is thick peeling, and the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2% obtains pretreated pumpkin;
Described softening making beating is pretreated pumpkin to be placed in to boiling at l00 ℃ make the slaking of melon meat, and makes enzyme deactivation, in the melon meat after slaking, adds the water of 10 times of quality and the powder-beta-dextrin of melon meat quality 4%, obtains crushed pumpkin, to remove the astringent taste of pumpkin after making beating;
Described enzymolysis is the pH value 3.8 of adjusting crushed pumpkin, adds the pectase of crushed pumpkin quality 0.06% and the cellulase of crushed pumpkin quality 0.12%, in 55 ℃ of enzymolysis 90min;
The described enzyme that goes out be by the crushed pumpkin after enzymolysis in 100 ℃ of heating 20min enzymes that go out, then use 200 order filter-cloth filterings, remove filter residue and obtain Pumpkin Juice.
Described aloe is that aloe condensed juice and guava inspissated juice are mixed with guava mixed juice, obtains after being deployed into 100% original fruit juice with drinking water.Wherein can make by oneself also can be commercial for aloe condensed juice and guava inspissated juice.
In preparation industry for inspissated juice, adopt unified way, fresh fruit obtains original fruit juice through squeezing the juice, after degumming process and ultrafiltration, under higher vacuum in the 60-65 ℃ of moisture evaporating in original fruit juice, if it is 6 that volume narrows down to 1/6 inspissated juice cycles of concentration of original fruit juice volume, if it is 10 that volume narrows down to 1/10 inspissated juice cycles of concentration of original fruit juice volume, by that analogy, obtain inspissated juice.
In the present invention, aloe condensed juice and guava inspissated juice are all purchased from Huai’an oersted Food Co., Ltd.
So-called 100% original fruit juice, the inspissated juice that the cycles of concentration of take is 6 times is example, refers to and adds the inspissated juice of 1 mass parts and the allotment of the drinking water of 5 mass parts; The inspissated juice that the cycles of concentration of take is again 10 times is example, refers to and adds the inspissated juice of 1 mass parts and the allotment of the drinking water of 9 mass parts, by that analogy.
The Cactus juice of proportional quantity, leprosy grape juice, Bitter Melon Juice, Pumpkin Juice, aloe are mixed to obtain to compound with guava mixed juice, xylitol, honey and compound stabilizer, with superhigh-voltage homogenizing machine by compound homogeneous, homogenization pressure is 20-40MPa, temperature is 65 ℃, degassed 5-10 minute under the vacuum condition of 80kPa subsequently, in 95 ℃ of sterilization 20min, be cooled to room temperature again, sterile filling gets product.
Research shows, contains abundant trace element, protein, amino acid, vitamin, polysaccharide, flavonoids and pectin etc. in cactus fruit, and wherein cactus pear fruit polysaccharides has significant hypoglycemic activity.The traditional Chinese medical science thinks that prickly pear is lightly seasoned cold in nature, has the effect such as calm the nerves of the promoting flow of qi and blood circulation, clearing damp myogenic, alleviating pain and detumescence, clearing heat and detoxicating, invigorating the spleen to arrest diarrhea, the diuresis of calming the nerves, stomach invigorating nourishing, antibechic.Modern medicine study shows, prickly pear has anti-cancer and inhibiting tumor, hypoglycemic, reducing blood lipid, hypotensive, antioxidation activity and regulates fat metabolism, dietary fiber is provided, strengthens function of intestinal canal, eliminates the effects such as oedema, removing toxic substances, raising immunity.
Leprosy grape is rich in the several mineral materials compositions such as amino acid, alkaloid, flavonoids, polypeptide, polysaccharide, saponin, crude fibre, vitamin C and calcium, phosphorus, iron.Research shows, leprosy grape water extract has obvious hypoglycemic activity.
Balsam pear is rich in protein, carbohydrate, saponin, para-insulin peptide, phosphorus, calcium, iron, potassium, carrotene, V
b1, V
b2, V
b5, V
cetc. nutritional labeling, wherein the composition such as bitter melon polysaccharide, momordica saponins and para-insulin peptide has obvious effect of lowering blood sugar.The traditional Chinese medical science is thought, balsam pear bitter cold in nature has the effects such as clearing heat and detoxicating, pure and fresh improving eyesight, hypoglycemic, anti-inflammatory.That its pharmacologically active is mainly reflected in is hypoglycemic, anti-AIDS, antitumor, antifertility, antibacterial, antiviral and on aspects such as immunocompetence impacts.
Pumpkin nutrient is abundant, is rich in pectin, pentosan, mannose, several amino acids, vitamin, mineral matter and alkaloid (cucurbitine, trigonelline) etc.Modern study shows: pumpkin can promote the secretion of human insulin, and the secretion of insulin in human body has good prevention effect to diabetes.Can increase the power of regeneration of liver, nephrocyte, the diseases such as control chronic diabetes, hypertension, smooth away wrinkles, skin care, accelerate litholysis, eliminate unnecessary cholesterol in human body, prevent and treat the effects such as artery sclerosis simultaneously.
In aloe, contain abundant amino acid, polysaccharide, vitamin and multiple inorganic mineral substance element; wherein polysaccharide has significant hypoglycemic activity; also have in addition antitumor, radioresistance, anti-inflammatory, anti-oxidant, protection liver, regulate the effects such as immunity, clearing away heat and loosing the bowels, medicinal history is long.
Guava fruit is nutritious, containing higher V
a, V
cand cellulosic, be rich in the trace elements such as protein, polysaccharide, flavonoids and phosphorus, potassium, iron, calcium, magnesium, wherein polysaccharide and flavonoids have obvious hypoglycemic activity, in addition, also there is astringing to arrest diarrhea, antipruritic, anti-oxidant anti-aging, balance blood sugar, clearing liver, Chronic disease prevention, the metabolism of promotion human body, keep capilary normally unimpeded, help digestion, the effect such as appetizing, defaecation.
Therefore, utilize cactus fruit, leprosy grape, balsam pear, pumpkin, aloe, guava exploitation to there is the health drink of effect of lowering blood sugar, not only met modern's demand, and greatly improved the added value of raw material.Up to now, the research that utilizes prickly pear to make composite health care beverage both at home and abroad there is not yet report.
Function of blood sugar reduction beverage of the present invention is allocated the raw materials such as prickly pear, leprosy grape, balsam pear, pumpkin, aloe and guava in rational ratio, make the active ingredient of different material fully merge complementation, the more single raw material better effects if of function of blood sugar reduction, when playing prevention and health care effect, can ancillary drug treatment diabetes.
Hypoglycemic beverage of the present invention sour-sweet tasty and refreshing, color and luster is even, not only can be used as general population's daily beverage, can also be as the functional beverage of the special populations such as diabetes patient.
Organoleptic indicator of the present invention: color and luster is aubergine, uniformity, has the distinctive natural faint scent of compound fruit and vegetable juice, and has prickly pear and the distinctive flavour of compound fruit and vegetable juice, sour-sweet tasty and refreshing.Outward appearance is without obvious sediment, not stratified, the Cloudy fruit-vegetable juice beverage of good fluidity, meets the compound fruit and vegetable juice beverage of national fruit-vegetable juice beverage sanitary standard GB19297-2003, can meet the sour-sweet salubrious mouthfeel of compound fruit and vegetable juice beverage, there is again hypoglycemic function.
Beneficial effect of the present invention is mainly reflected in:
1, the present invention has developed cactus fruit beverage first, has enriched the processing and utilization of prickly pear
At present prickly pear is at ground aboundresources such as China Hainan, Guangdong, Guangxi, but do not carry out rational exploitation and utilization, and is wild, not yet concentrates large-scale planting production.The present invention be take prickly pear first as raw material research and development health drink, has widened the range of application of prickly pear, has good prospects.
2, the present invention has developed leprosy grape class beverage first, has enriched the processing and utilization of leprosy grape
The leprosy grape that China produces is at present a kind of wild edible melon and fruit, does not also carry out large-scale planting production.According to the literature, leprosy grape water extract has significant hypoglycemic activity, but is not developed to so far function of blood sugar reduction beverage.The present invention is used for developing hypoglycemic health drink by leprosy grape first, has widened the range of application of leprosy grape, has good prospects.
3, compound fruit and vegetable juice beverage effect of lowering blood sugar of the present invention is than single raw material better effects if
Function of blood sugar reduction beverage of the present invention is allocated the raw materials such as prickly pear, leprosy grape, balsam pear, pumpkin, aloe and guava in rational ratio, make the active ingredient of different material fully merge complementation, the more single raw material of effect of lowering blood sugar is better, when playing prevention and health care effect, can ancillary drug treatment diabetes.
4, the present invention has enriched the kind of beverage
Traditional beverage raw material kind is single, and nutritive value is not high, and function specific aim is not strong, especially less for the beverage of the special populations such as diabetes patient.It is raw material that the present invention adopts prickly pear, leprosy grape, balsam pear, pumpkin, aloe, the guava of integration of drinking and medicinal herbs, not only nutritious, and price can be accepted extensively by consumer.Due to the integration of drinking and medicinal herbs of raw material, using it as health fruit-vegetable juice beverage, exploitation, brings into play its health-care efficacy, has compared with profound significance.
Four, the specific embodiment
Below in conjunction with embodiment, the present invention is further described.
In the embodiment of the present invention, aloe condensed juice and guava inspissated juice are all purchased from Huai’an oersted Food Co., Ltd.
Embodiment 1:
The preparation method with the compound fruit and vegetable juice beverage of effect of lowering blood sugar, comprises following making step:
(1) preparation of Cactus juice
1. cleaning treatment: by without going rotten, without disease and pest, the prickly pear fresh fruit deburring that has no mechanical damage, and clean with clear water, obtain clean prickly pear;
2. the lixiviate of pulling an oar: will clean prickly pear and add water making beating, clean prickly pear is 1:10 with the mass ratio of water, remains on lixiviate 10-15min at the temperature of 65-75 ℃, and then mistake 200 order filter clothes, obtain Cactus juice.
(2) preparation of leprosy grape juice
1. pretreatment: fresh leprosy grape is cleaned, remove surperficial silt, by going the leprosy grape after seed to be cut into the sheet piece that 0.5cm is thick, drop into immediately the hot water blanching 5-8 minute of 85-95 ℃;
2. crushing and beating: pretreated leprosy grape sheet piece is cooled to normal temperature, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2%, be crushed to the pectase that adds broken leprosy grape quality 0.1% after granular size fine and even, standing 2h under 35 ℃ of temperature, pH5.5, obtains leprosy grape slurry after defibrination;
3. enzyme goes out: leprosy grape slurry is heated to the 20min enzyme that goes out at 100 ℃, and with 200 order filter-cloth filterings, removal filter residue obtains leprosy grape juice.
(3) preparation of Bitter Melon Juice
1. pretreatment: fresh balsam pear is cleaned, remove surperficial silt, will go the balsam pear after flesh to be cut into the sheet piece that 0.5cm is thick, drop into immediately hot water blanching 5-8 minute of 85-95 ℃ to protect look;
2. remove bitter taste: pretreated balsam pear slice is put in 10% salt solution and soaks 30-50 minute, take out and be placed in boiling water rinsing 1 minute, utilize the bitter principle effect in salt and balsam pear to remove bitter taste, repeat three desalinations of rinsing;
3. crushing and beating: will go the balsam pear slice after bitter taste to be cooled to normal temperature, be crushed to the pectase standing 2h under 35 ℃ of temperature, pH5.5 that adds melon meat quality 0.1% after granular size fine and even, obtain balsam pear slurry after defibrination;
4. enzyme goes out: the balsam pear after enzymolysis is starched and at 100 ℃, heated the 20min enzyme that goes out, with 200 order filter-cloth filterings, remove filter residue and obtain Bitter Melon Juice.
(4) preparation of Pumpkin Juice
1. pretreatment: pumpkin raw material is cleaned, removed seed flesh, be cut into the sheet piece that 5mm is thick, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2%;
2. softening making beating: pretreated pumpkin sheet is placed in to boiling at temperature l00 ℃ and makes the slaking of melon meat, and make enzyme deactivation.Ratio with 1:10 adds water, and pulls an oar after adding the powder-beta-dextrin of melon meat quality 4%, to remove the astringent taste of pumpkin, makes crushed pumpkin;
3. enzymolysis: crushed pumpkin is added to the pectase of crushed pumpkin quality 0.06% and 0.12% cellulase when the pH3.8, keep 55 ℃ of enzymolysis 90min of temperature;
4. enzyme goes out: the crushed pumpkin after enzymolysis is heated at 100 ℃ to the 20min enzyme that goes out, with 200 order filter-cloth filterings, remove filter residue and obtain Pumpkin Juice.
(5) preparation of aloe and guava mixed juice
Aloe condensed juice and guava inspissated juice are deployed into 100% original fruit juice in concentrated ratio with drinking water.
(6) allotment: by 0.1 part of 30 parts of Cactus juices, 10 parts of leprosy grape juices, 15 parts of Bitter Melon Juices, 5 parts of Pumpkin Juice, aloe and 10 parts of guava mixed juices, 4 parts of xylitols, 0.4 part of honey, the compound stabilizer that mixes in sodium alginate, pectin and the CMC-Na of the mediation of 1:1:2 ratio, mix to obtain compound;
(7) homogeneous: by compound homogeneous, homogenization pressure is 20-40MPa with superhigh-voltage homogenizing machine, and temperature is 65 ℃;
(8) degassed: under the vacuum condition of 80kPa, the degassed 5-10 minute of feed liquid;
(9) sterilization, cooling, filling: the beverage after degassed is placed in to retort, and 95 ℃ of sterilization 20min, are cooled to room temperature, aseptic packaging.
Embodiment 2:
The preparation method with the compound fruit and vegetable juice beverage of effect of lowering blood sugar, comprises following making step:
(1) preparation of Cactus juice
1. cleaning treatment: by without going rotten, without disease and pest, cactus fruit (fresh fruit) deburring that has no mechanical damage, and clean with clear water, obtain clean prickly pear;
2. the lixiviate of pulling an oar: will clean prickly pear and add water making beating, clean prickly pear is 1:10 with the mass ratio of water, remains on lixiviate 10-15min at the temperature of 65-75 ℃, and then mistake 200 order filter-cloth filterings, obtain Cactus juice.
(2) preparation of leprosy grape juice
1. pretreatment: fresh leprosy grape is cleaned, remove surperficial silt, by going the leprosy grape after seed to be cut into the sheet piece that 0.5cm is thick, drop into immediately the hot water blanching 5-8 minute of 85-95 ℃;
2. crushing and beating: pretreated leprosy grape sheet piece is cooled to normal temperature, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2%, be crushed to the pectase that adds broken leprosy grape quality 0.1% after granular size fine and even, standing 2h under 35 ℃ of temperature, pH5.5, obtains leprosy grape slurry after defibrination;
3. enzyme goes out: leprosy grape slurry is heated to the 20min enzyme that goes out at 100 ℃, and with 200 order filter-cloth filterings, removal filter residue obtains leprosy grape juice.
(3) preparation of Bitter Melon Juice
1. pretreatment: fresh balsam pear is cleaned, remove surperficial silt, will go the balsam pear after flesh to be cut into the sheet piece that 0.5cm is thick, drop into immediately hot water blanching 5-8 minute of 85-95 ℃ to protect look;
2. remove bitter taste: pretreated balsam pear slice is put in 10% salt solution and soaks 30-50 minute, take out and be placed in boiling water rinsing 1 minute, utilize the bitter principle effect in salt and balsam pear to remove bitter taste, repeat three desalinations of rinsing;
3. crushing and beating: will go the balsam pear slice after bitter taste to be cooled to normal temperature, be crushed to the pectase standing 2h under 35 ℃ of temperature, pH5.5 that adds melon meat quality 0.1% after granular size fine and even, obtain balsam pear slurry after defibrination;
4. enzyme goes out: the balsam pear after enzymolysis is starched and at 100 ℃, heated the 20min enzyme that goes out, with 200 order filter-cloth filterings, remove filter residue and obtain Bitter Melon Juice.
(4) preparation of Pumpkin Juice
1. pretreatment: pumpkin raw material is cleaned, removed seed flesh, be cut into the sheet piece that 5mm is thick, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2%;
2. softening making beating: pretreated pumpkin sheet is placed in to boiling at temperature l00 ℃ and makes the slaking of melon meat, and make enzyme deactivation.Ratio with 1:10 adds water, and pulls an oar after adding the powder-beta-dextrin of melon meat quality 4%, to remove the astringent taste of pumpkin, makes crushed pumpkin;
3. enzymolysis: crushed pumpkin is added to the pectase of crushed pumpkin quality 0.06% and 0.12% cellulase when the pH3.8, keep 55 ℃ of enzymolysis 90min of temperature;
4. enzyme goes out: the crushed pumpkin after enzymolysis is heated at 100 ℃ to the 20min enzyme that goes out, with 200 order filter-cloth filterings, remove filter residue and obtain Pumpkin Juice.
(5) preparation of aloe and guava mixed juice
Aloe condensed juice and guava inspissated juice are deployed into 100% original fruit juice in concentrated ratio with drinking water.
(6) allotment: by 0.3 part of 10 parts of Cactus juices, 20 parts of leprosy grape juices, 5 parts of Bitter Melon Juices, 15 parts of Pumpkin Juice, aloe and 20 parts of guava mixed juices, 8 parts of xylitols, 0.6 part of honey, the compound stabilizer that mixes in sodium alginate, pectin and the CMC-Na of the mediation of 1:1:2 ratio, mix to obtain compound;
(7) homogeneous: by compound homogeneous, homogenization pressure is 20-40MPa with superhigh-voltage homogenizing machine, and temperature is 65 ℃;
(8) degassed: under the vacuum condition of 80kPa, the degassed 5-10 minute of feed liquid;
(9) sterilization, cooling, filling: the beverage after degassed is placed in to retort, and 95 ℃ of sterilization 20min, are cooled to room temperature, aseptic packaging.
Embodiment 3:
The preparation method with the compound fruit and vegetable juice beverage of effect of lowering blood sugar, comprises following making step:
(1) preparation of Cactus juice
1. cleaning treatment: by without going rotten, without disease and pest, cactus fruit (fresh fruit) deburring that has no mechanical damage, and clean with clear water, obtain clean prickly pear;
2. the lixiviate of pulling an oar: will clean prickly pear and add water making beating, clean prickly pear is 1:10 with the mass ratio of water, remains on lixiviate 10-15min at the temperature of 65-75 ℃, and then mistake 200 order filter-cloth filterings, obtain Cactus juice.
(2) preparation of leprosy grape juice
1. pretreatment: fresh leprosy grape is cleaned, remove surperficial silt, by going the leprosy grape after seed to be cut into the sheet piece that 0.5cm is thick, drop into immediately the hot water blanching 5-8 minute of 85-95 ℃;
2. crushing and beating: pretreated leprosy grape sheet piece is cooled to normal temperature, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2%, be crushed to the pectase that adds broken leprosy grape quality 0.1% after granular size fine and even, standing 2h under 35 ℃ of temperature, pH5.5, obtains leprosy grape slurry after defibrination;
3. enzyme goes out: leprosy grape slurry is heated to the 20min enzyme that goes out at 100 ℃, and with 200 order filter-cloth filterings, removal filter residue obtains leprosy grape juice.
(3) preparation of Bitter Melon Juice
1. pretreatment: fresh balsam pear is cleaned, remove surperficial silt, will go the balsam pear after flesh to be cut into the sheet piece that 0.5cm is thick, drop into immediately hot water blanching 5-8 minute of 85-95 ℃ to protect look;
2. remove bitter taste: pretreated balsam pear slice is put in 10% salt solution and soaks 30-50 minute, take out and be placed in boiling water rinsing 1 minute, utilize the bitter principle effect in salt and balsam pear to remove bitter taste, repeat three desalinations of rinsing;
3. crushing and beating: will go the balsam pear slice after bitter taste to be cooled to normal temperature, be crushed to the pectase standing 2h under 35 ℃ of temperature, pH5.5 that adds melon meat quality 0.1% after granular size fine and even, obtain balsam pear slurry after defibrination;
4. enzyme goes out: the balsam pear after enzymolysis is starched and at 100 ℃, heated the 20min enzyme that goes out, with 200 order filter-cloth filterings, remove filter residue and obtain Bitter Melon Juice.
(4) preparation of Pumpkin Juice
1. pretreatment: pumpkin raw material is cleaned, removed seed flesh, be cut into the sheet piece that 5mm is thick, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2%;
2. softening making beating: pretreated pumpkin sheet is placed in to boiling at temperature l00 ℃ and makes the slaking of melon meat, and make enzyme deactivation.Ratio with 1:10 adds water, and pulls an oar after adding the powder-beta-dextrin of melon meat quality 4%, to remove the astringent taste of pumpkin, makes crushed pumpkin;
3. enzymolysis: crushed pumpkin is added to the pectase of crushed pumpkin quality 0.06% and 0.12% cellulase when the pH3.8, keep 55 ℃ of enzymolysis 90min of temperature;
4. enzyme goes out: the crushed pumpkin after enzymolysis is heated at 100 ℃ to the 20min enzyme that goes out, with 200 order filter-cloth filterings, remove filter residue and obtain Pumpkin Juice.
(5) preparation of aloe and guava mixed juice
Aloe condensed juice and guava inspissated juice are deployed into 100% original fruit juice in concentrated ratio with drinking water.
(6) allotment: by 0.2 part of 20 parts of Cactus juices, 15 parts of leprosy grape juices, 10 parts of Bitter Melon Juices, 10 parts of Pumpkin Juice, aloe and 15 parts of guava mixed juices, 6 parts of xylitols, 0.5 part of honey, the compound stabilizer that mixes with sodium alginate, pectin and the CMC-Na of 1:1:2 ratio mediation, mix to obtain compound;
(7) homogeneous: by compound homogeneous, homogenization pressure is 20-40MPa with superhigh-voltage homogenizing machine, and temperature is 65 ℃;
(8) degassed: under the vacuum condition of 80kPa, the degassed 5-10 minute of feed liquid;
(9) sterilization, cooling, filling: the beverage after degassed is placed in to retort, and 95 ℃ of sterilization 20min, are cooled to room temperature, aseptic packaging.
The results of animal of compound fruit and vegetable juice beverage of the present invention is as follows:
(1) modeling of STZ diabetes rat model and grouping
100 of adult healthy SD rats, body weight 179.56 ± 3.07(g), male and female half and half, fasting 12h before modeling, afterbody is got hematometry fasting blood sugar, chooses blood glucose value the rat of 4.1-6.1mmo1/L, with 10 as Normal group, all the other all use the STZ modeling of 80mg/kg, survey fasting blood sugar after 72h, and the rat of all blood glucose value >=13mmol/L is all regarded as diabetes animal model.Get 50 of rats after modeling success, be divided into 5 groups, be respectively model group, insulin group, Cactus juice group, compound fruit and vegetable juice L group (low dose group), compound fruit and vegetable juice M group (middle dosage group), compound fruit and vegetable juice H group (high dose group), 10 every group.
(2) dosage and method
Insulin group intramuscular injection insulin 10U.kg
-1.d
-1, Cactus juice group adds 10 times of water by prickly pear deburring and squeezes the juice and gavage 2.00g.kg
-1.d
-1, the beverage that compound fruit and vegetable juice L group, compound fruit and vegetable juice M group and compound fruit and vegetable juice H group are made by the beverage proportioning in embodiment 3 respectively gavage 1.50g.kg
-1.d
-1, 2.00g.kg
-1.d
-1, 2.50g.kg
-1.d
-1.All rats all on the feed on the basis of conventional feed, give respectively different gavage liquid by requirement of experiment.Every morning empty stomach administration 1 time, gavage is 8 weeks continuously.
(3) mainly detect index and result
1. general index:
Note observing also periodic logging and respectively organize rat body weight situation of change.In table 1, recording result shows, Cactus juice group, compound fruit and vegetable juice L group, compound fruit and vegetable juice M group and compound fruit and vegetable juice H group all have improvement effect in various degree to the diabetic symptom of diabetes rat, the improvement effect of compound fruit and vegetable juice M group and compound fruit and vegetable juice H group is more remarkable compared with Cactus juice group, and presents dose-dependent effect and time-dependent effect.Result demonstration, compound fruit and vegetable juice drinks of the present invention is better than single Cactus juice to the improvement effect of diabetes rat symptom.
The impact of table 1 on STZ diabetes rat changes of weight
Note:
the * P < 0.05 that compares with model group, * * P < 0.01; Compare △ P < 0.05, △ △ P < 0.01 with Cactus juice group.
2. the mensuration of blood sugar
After modeling success, divide into groups and measure initial fasting blood sugar (FBG), second day starts administration, later respectively medication the 4th week and the 8th week, measures and respectively organizes blood glucose value, calculates and the impact of statistical analysis medicine on STZ blood glucose in diabetic rats level.In table 2, result shows, with model group comparison, Cactus juice group, compound fruit and vegetable juice L group, compound fruit and vegetable juice M group and compound fruit and vegetable juice H group, the fasting blood sugar of diabetes rat all has reduction in various degree, and compound fruit and vegetable juice group hypoglycemic effect presents dose-dependent effect and time-dependent effect.Wherein the hypoglycemic effect of compound fruit and vegetable juice M group and compound fruit and vegetable juice H group is better than Cactus juice group.
Table 2STZ diabetes rat fasting blood glucose level
Note:
the * P < 0.05 that compares with model group, * * P < 0.01; Compare △ P < 0.05, △ △ P < 0.01 with Cactus juice group.
3. the mensuration of glycosylated hemoglobin (GHb)
After administration 8 weeks, measure the saccharification hemoglobin content of respectively organizing STZ diabetes rat, calculate and the impact of statistical analysis on diabetes rat glycosylated hemoglobin level.In table 3, result shows, compares with model group, and the STZ diabetes rat saccharification hemoglobin content of Cactus juice group, compound fruit and vegetable juice L group, compound fruit and vegetable juice M group and compound fruit and vegetable juice H group all significantly reduces (P < 0.01).And the saccharification hemoglobin content of compound fruit and vegetable juice M group and compound fruit and vegetable juice H group rat is significantly lower than Cactus juice group.
The table 3 administration 8 weeks impact on STZ diabetes rat glycosylated hemoglobin
Note:
the * P < 0.05 that compares with model group, * * P < 0.01; Compare △ P < 0.05, △ △ P < 0.01 with Cactus juice group.
4. anxious toxicity test
According to the dose of the tolerant Cmax of animal, maximum volume, every 12h administration 1 time, administration is 2 weeks altogether.By acute toxicity test conventional method gavage.Observe in test, in mouse gavaging medicine 2 weeks, Cactus juice group, compound fruit and vegetable juice L group, compound fruit and vegetable juice M group, compound fruit and vegetable juice H group and blank treated animal are without extremely, after 2 weeks, put to death mouse, dissect, observe the heart, liver, spleen, lung, kidney, brain, small intestine, all no abnormal.
Above pharmacological effect is tested and animal acute toxicity test shows, compound fruit and vegetable juice beverage of the present invention has the effect that reduces STZ diabetes rat model blood sugar, and edible safety has no side effect.Therefore, the present invention not only provides a kind of approach of adjuvant therapy of diabetes, and normal person drinks and can also play good prevention diabetes effect.
Claims (1)
1. a compound fruit and vegetable juice beverage with effect of lowering blood sugar, is characterized in that its raw material is configured to by mass fraction:
Cactus juice 10-30 part, leprosy grape juice 10-20 part, Bitter Melon Juice 5-15 part, Pumpkin Juice 5-15 part, aloe and guava mixed juice 10-20 part, xylitol 4-8 part, honey 0.4-0.6 part, compound stabilizer 0.1-0.3 part;
Described compound stabilizer is to be mixed and form by the mass ratio of 1:1:2 by sodium alginate, pectin and CMC-Na;
Described Cactus juice is that the fresh prickly pear by deburring and after cleaning is mixed according to the ratio of mass ratio 1:10 with water, after making beating at 65-75 ℃ lixiviate 10-15min, the Cactus juice obtaining after mistake 200 order filter clothes;
Described leprosy grape juice is the leprosy grape juice obtaining after pretreatment, crushing and beating and the enzyme that goes out; Described pretreatment be by remove seed and clean after the fresh leprosy grape piece of cutting into slices, add blanching 5-8 minute in the water of 85-95 ℃ to obtain pretreated leprosy grape; Described crushing and beating is that pretreated leprosy grape is cooled to normal temperature, the citric acid colour protecting liquid that adds the mass concentration 0.2% of 3 times of quality, the pectase that adds broken leprosy grape quality 0.1% after fragmentation, standing 2h under 35 ℃, pH5.5, obtains leprosy grape slurry after making beating; The described enzyme that goes out is that described leprosy grape is starched in 100 ℃ of heating 20min enzymes that go out, then uses 200 order filter-cloth filterings, removes filter residue and obtains leprosy grape juice;
Described Bitter Melon Juice is through pretreatment, removes the Bitter Melon Juice that obtains after bitter taste, crushing and beating and the enzyme that goes out; Described pretreatment is by the piece of cutting into slices of the fresh balsam pear after going flesh to clean, and drops into hot water blanching 5-8 minute of 85-95 ℃ to protect look, obtains pretreated balsam pear; The described bitter taste that goes is that pretreated balsam pear is placed in to mass concentration is that 10% NaCl solution soaks 30-50min, takes out and is placed in boiling water rinsing 2-3 time; Described crushing and beating is to go the balsam pear after bitter taste to be cooled to normal temperature, to add the pectase of broken balsam pear quality 0.1% after fragmentation, and standing 2h under 35 ℃, pH5.5 obtains balsam pear slurry after making beating; The described enzyme that goes out is that described balsam pear is starched in 100 ℃ of heating 20min enzymes that go out, then uses 200 order filter-cloth filterings, removes filter residue and obtains Bitter Melon Juice;
Described Pumpkin Juice is the Pumpkin Juice obtaining after pretreatment, softening making beating, enzymolysis and the enzyme that goes out; Described pretreatment be peeling is removed to seed flesh and clean after the pumpkin piece of cutting into slices, the citric acid colour protecting liquid that the mass concentration that adds 3 times of quality is 0.2% obtains pretreated pumpkin; Described softening making beating is pretreated pumpkin to be placed in to boiling at l00 ℃ make the slaking of melon meat, in the melon meat after slaking, adds the water of 10 times of quality and the powder-beta-dextrin of melon meat quality 4%, obtains crushed pumpkin after making beating; Described enzymolysis is the pH value 3.8 of adjusting described crushed pumpkin, adds the pectase of crushed pumpkin quality 0.06% and the cellulase of crushed pumpkin quality 0.12%, in 55 ℃ of enzymolysis 90min; The described enzyme that goes out be by the crushed pumpkin after enzymolysis in 100 ℃ of heating 20min enzymes that go out, then use 200 order filter-cloth filterings, remove filter residue and obtain Pumpkin Juice;
Described aloe is that aloe condensed juice and guava inspissated juice are mixed with guava mixed juice, obtains after being deployed into 100% original fruit juice with drinking water.
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