CN104887713A - Preparing method of oyster active extract having functions of producing sperm to tonify the kidney and improving sexual function - Google Patents
Preparing method of oyster active extract having functions of producing sperm to tonify the kidney and improving sexual function Download PDFInfo
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Abstract
The invention discloses a preparing method of oyster active extract having functions of producing sperm to tonify the kidney and improving sexual function. The oyster active extract has the functions of producing the sperm to tonify the kidney and improving the sexual function. A composition mainly comprising the oyster active extract as a main active ingredient further comprises Epimedium herb, astragalus root, root of hairy asiabell, fruit of Chinese wolfberry, common Macrocarpium fruit, prepared rehmannia root, thinleaf milkwort root-bark, rhizome of oriental water plantain, licorice root and Indian bread. The preparing method includes special debitterizing, papain hydrolysis, fermentation-based deodorization via activated carbon and yeast, secondary debitterizing via beta-cyclodextrin embedding with mycose, decoloring via diatomite, color optimizing via bamboo leaf flavonoid and neutral sodium phytate, and taste optimizing via compound flavoring (comprising glucose, fructo-oligosaccharide and xylitol). The preparing method has the advantages that the process is advanced, the formula is scientific and reasonable, the nutrients are complete, the fishy smell is slight, the composition has natural flavor and the functions of producing the sperm to enhance the kidney, resisting fatigue, improving the sexual function and enhancing the body immunity, and the composition assists in treating symptoms, such as impotence, spermatorrhea and premature ejaculation, caused by deficiency of kidney-yang or deficiency of kidney-yin.
Description
Technical field
The present invention relates to a kind of preparation method of marine organism health food, is a kind of spermatogenesis the kidney invigorating, the Concha Ostreae activity extract preparation method improving sexual function specifically.
Background technology
Concha Ostreae (oyster), be commonly called as large oyster, Carnis ostreae delicious, nutritious, there is the title of " marine milk ", it is one of China's health care remedy diet being classified as integration of edible and medicinal herbs in the first batch, containing rich in protein, vitamin, trace element, and composite phospholipid, taurine, phosphoinositide, eicosapentaenoic acid (EPA), docosahexenoic acid (DHA) isoreactivity composition, essential amino acids completeness contained by it and mass ratio are better than Lac Bovis seu Bubali and human milk, Compendium of Material Medica record: Carnis ostreae " polyphagia it, kidney invigorating and YANG supporting, and can void be controlled, separate erysipelas ".Materia Medica of Decoction is called: " Concha Ostreae, be the agent of softening the hard mass, the medicine of this kidney channel blood system also ", has the effect of spermatogenesis kidney tonifying to male, can the disease such as auxiliary therapy insufficiency of kidney-YANG or deficiency of the kidney yin and the male infertility that causes because of spermacrasia.
Current most of Concha Ostreae mainly directly eats, functional Concha Ostreae product is also fewer, listing product as: " extra large king's OYSTER EXTRACT CAPSULE " of Hai Wang group is for the protection of gastric mucosa, protection hepatocyte, the full nutrient tablet of Concha Ostreae (EXT) of Guangdong Zhuhai Ocean nourishing food company is for hard masses softening and resolving, reduce phlegm and moisturize, " OYSTER EXTRACT CAPSULE " is for adjuvant treating hepatitis, most hepatoprotective paying close attention to Concha Ostreae, improve immunity, the physiological function developmental researches such as blood fat reducing, and Concha Ostreae is strong for spermatogenesis, the unique function report improving male's performance high is few, but because modern society's life joint gathers quickening, pressure is large, cause numerous crowd appearance sexual dysfunction in various degree, the particularly weakness of the waist and knees of middle-aged male appearance, sexual impotence, seminal emission, the phenomenons such as premature ejaculation, increasingly general, so-called kidney invigorating and YANG supporting types of drugs is few on the market, less eutherapeutic, manyly also to need and chemical medicine coupling, mostly there is side effect, as face flushing, vasodilation, feel dizzy, even also sexual dysfunction can be increased the weight of.
The traditional Chinese medical science thinks that the kidney being the origin of congenital constitution, and insufficiency of kidney-YANG is soreness of the waist and knees then, impotence and premature ejaculation; dysuria, although reason is numerous, its basic pathogenesis many because of natural endowment not enough; the day after tomorrow sexual strain, or juvenile masturbation, long-term psychentonia; excessive thinking, the pent-up of feelings will, for liver, kidney, the heart, spleen are impaired; negative and positive of qi and blood virtual loss, yin collaterals cervical carcinoma with cachexia, or stagnation of liver-QI dampness; meridians lose smooth, cause penis to form.Kidney storing essence, main reproduction, has one's ideas straightened out cloudy with two, and the muscles along the regular meridians of spleen all gathers and external genitals, and deficiency of kidney essence, deficiency-weakness of spleen-QI, then should with reinforcing the kidney and supporting YANG, and invigorating the spleen and benefiting QI is main.Therefore how the Concha Ostreae of integration of edible and medicinal herbs and Chinese medicine are made a kind of unique flavor through reasonable compatibility, aminoacid composition is perfect, be of high nutritive value and fishy smell less, to be easy to accept by consumer, be applied to spermatogenesis kidney tonifying, improve the Concha Ostreae active extract composition of male's sexual there is tempting development prospect.
Summary of the invention
The present invention is intended to solve the problem, there is provided a kind of technique advanced, fishy smell is little, local flavor nature, nutritional labeling are complete, content height Concha Ostreae active extract composition preparation method, the Concha Ostreae active extract composition obtained has the admittedly true effect of replenishing essence, can be used for the soreness of waist that auxiliary therapy insufficiency of kidney-YANG or deficiency of the kidney yin cause, sexual impotence, seminal emission, premature ejaculation, improve male's sexual, safe secondary effect is little.
A Concha Ostreae activity extract preparation method for spermatogenesis the kidney invigorating, raising sexual function, comprises the steps:
1) Concha Ostreae debitterize process: add 1 times of weight 0.5%Na to fresh Concha Ostreae software
2cO
3solution, soaks, and filters, with 2-3 times of weight pure water, and the Concha Ostreae software part repeatedly after washing and filtering;
2) homogenate, enzymolysis: to step 1) add the pure water of 2 times of weight in the Concha Ostreae software that obtains, make homogenate, regulate pH6-7, add papain enzymolysis, enzymolysis terminates, enzyme denaturing; Repeat above enzymolysis step 2-3 time, merge enzymolysis solution, cooling, centrifugal;
3) Concha Ostreae enzymolysis solution eliminating sargassum smell: first with active carbon to step 2) enzymolysis solution that obtains carries out first time eliminating sargassum smell, then carries out second time eliminating sargassum smell with yeast;
4) Concha Ostreae enzymolysis solution secondary debitterize: to step 3) the eliminating sargassum smell enzymolysis solution that obtains adds beta-schardinger dextrin-and carries out embedding debitterize;
5) decolour: add kieselguhr decolouring, filter;
6) concentrated: by step 5) filtrate that obtains concentrates;
7) allocate: by step 6) concentrated solution that obtains is mixed in proportion with Chinese medicine extraction liquid, stirs, and add and mix browning inhibitor, composite flavor, mixing preparation, filtration, subpackage, sterilizing obtain finished product;
Described Chinese medicine extraction liquid is obtained by following steps: by Herba Epimedii 5-10 part of following weight portion, Radix Astragali 3-8 part, Radix Codonopsis 3-5 part, Fructus Corni 3-8 part, Radix Rehmanniae Preparata 1-3 part, Fructus Lycii 3-8 part, Radix Polygalae 5-8 part, Rhizoma Alismatis 3-5 part, Radix Glycyrrhizae 3-8 part, Poria 1-5 part, after mixing, add and be equivalent to raw material of Chinese medicine weight 8-10 pure water reflux, extract, doubly, each 1 hour, extract twice, merge extractive liquid, microwave radiation extraction liquid 10min, power 500W, carry out cryopreservation 6-8h, temperature 0-4 DEG C at once, filter, centrifugal, obtain supernatant;
Described mixing browning inhibitor is Folium Bambosae flavone 0.08% (w/v), neutral sodium phytate 0.05% (w/v), xanthan gum 0.2% (w/v), pectin 0.2% (w/v);
Described composite flavor is by glucose: oligofructose: xylitol is counted 2:3:1 by weight and is mixed to get, and addition is 2-5% (w/v).
Step 1 of the present invention) described in immersion, preferred 15-20min.
Step 2) described in enzymolysis, the amount preferably adding papain is by weight 800-1000U/g, hydrolysis temperature 50 DEG C, and enzymolysis time 4h, is heated to 90-100 DEG C, enzyme denaturing 10min, centrifugal preferred 3500r/min, 10min.
Step 3) described in eliminating sargassum smell, preferred active carbon addition is 2.0% (w/v), temperature 50 C, response time 30min; Yeast eliminating sargassum smell, yeast powder addition 1.5% (w/v), temperature 30 DEG C, fermentation time 20min.
Step 4) described in secondary debitterize, preferably add beta-schardinger dextrin-3% (w/v), stirred at ambient temperature 15min, fully mixes.
Step 5) described in kieselguhr decolouring, preferred kieselguhr addition 3% (w/v), control temperature 70 DEG C, process 1-2h.
Step 6) described in concentrated be preferably concentrated into moisture and be about 20%, product is faint yellow paste.
Step 7) described in concentrated solution be mixed in proportion with Chinese medicine extraction liquid and preferably mix according to the weight ratio of 15-20:80-85.
The invention still further relates to the Concha Ostreae active extract composition adopting above-mentioned preparation method to obtain.The present invention take Concha Ostreae as primary raw material, adopt modern biotechnology zymolysis technique and debitterizing and decoloring technology, overcome raw meat bitterness specific to marine product, be long placed in the problems such as brown stain, be aided with the Chinese medicines such as Herba Epimedii, the Radix Astragali, Radix Codonopsis, Fructus Lycii, Fructus Corni, Radix Rehmanniae Preparata, Radix Polygalae, Rhizoma Alismatis, Radix Glycyrrhizae, Poria again, with the addition of compound flavoring agent again to allocate, make nutritious, color is natural, taste flavor is pleasant, can be used for male's spermatogenesis strong, improve sexual function, safe and effective, have no side effect.
In order to obtain suitable mouthfeel, the Concha Ostreae active extract composition obtained also adds adjuvant (sorbic acid, Mel, sucrose, essence etc.) to be allocated, to mix edible denseness, taste etc., also can mix with pharmaceutical excipient or food additive, be prepared into any one dosage forms such as oral agents, capsule, granule, tablet, pill, electuary.
Compared to the prior art, the invention has the advantages that:
1, fresh Concha Ostreae software is added Na by the present invention
2cO
3solution soaks, and both can preserve internal organs complete, the bitterness of Concha Ostreae can be removed again, and retain delicate flavour, add water homogenate, adds papain and the proteolysis in Concha Ostreae is become little peptide, improve biological activity peptide content, decrease significant loss.
2, present invention employs the adsorption of active carbon to stench material, next utilizes yeast to combine with enzyme, using smell substance (macromole such as aldehyde, ketone) as substrate conversion be without bitter, without raw meat material; In addition yeast is also combined into macromolecular substances and assembles and remove with part fishy smell material, and the mouthfeel of the addition of yeast on extract has appreciable impact, the present invention is also optimized inoculum of dry yeast, obtains best culture propagation technique, thus reaches the object of eliminating sargassum smell.
3, because Concha Ostreae hydrolytic process can produce hydrophobicity bitter peptides, utilize in beta-schardinger dextrin-structure and there is a three-dimensional hydrophobic cavity, according to the coupling of molecular size, and Van der Waals force, hydrophobic forces and bitter peptides form clathrate, the quantity reducing bitter peptides alleviates bitterness, carry out secondary debitterize, work in coordination with composite flavor, mouthfeel is better.
4, be monarch drug with Concha Ostreae in Chinese prescription of the present invention, reinforcing the kidney and supporting YANG, mend marrow, restrain astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, with Herba Epimedii, the Radix Astragali, Radix Codonopsis, Fructus Lycii for ministerial drug, invigorating the spleen and replenishing QI, Fructus Corni, Radix Rehmanniae Preparata, Radix Polygalae are adjuvant drug, and negative and positive develop simultaneously, qi and blood tonifying, amount to reinforcing the kidney and supporting YANG, Rhizoma Alismatis, Radix Glycyrrhizae, Poria, for making medicine, are worked in coordination with and are played altogether, reach negative and positive and be harmonious, mend and not dry, effect of supplementing the kidney to control the nocturnal, YANG hyperactivity suppressing nourishing YIN.
5, the present invention adopts microwave radiation and cryopreservation Chinese medicine extraction liquid, effectively can reduce the bitter taste of Chinese medicine extract, then coordinate composite flavor, effectively cover the bitter taste of Chinese medicine extraction liquid, local flavor nature; Serious brown stain can be produced for aquatic product in processing (enzymolysis, sterilization and storage) process, affect product appearance and the problem causing quality to decline, adopt Folium Bambosae flavone to combine neutral sodium phytate Restrain browning.
Detailed description of the invention
With embodiment, the present invention will be described below, but the present invention is not limited to these embodiments.
Embodiment 1
A Concha Ostreae activity extract preparation method for spermatogenesis the kidney invigorating, raising sexual function, comprises the steps:
1) Concha Ostreae debitterize process: fresh Concha Ostreae is put into 1 times of weight 0.5%Na
2cO
3solution, soaks 15min, filters, and removes Na
2cO
3solution, adds 3 times of weight pure water, the Concha Ostreae software part repeatedly after washing and filtering;
2) homogenate, enzymolysis: to step 1) add the pure water of 2 times of weight in the Concha Ostreae software that obtains, homogenate is pounded with tissue mashing machine, regulate pH6, add papain enzymolysis, add papain 800U/g by weight, hydrolysis temperature 50 DEG C, enzymolysis time 4h, be heated to 90 DEG C, enzyme denaturing 10min alive; Repeat above enzymolysis step 2 times, merge enzymolysis solution, cooling, centrifugal, 3500r/min, 10min;
3) Concha Ostreae enzymolysis solution eliminating sargassum smell: first with active carbon to step 2) enzymolysis solution that obtains carries out first time eliminating sargassum smell, active carbon addition is 2.0%, temperature 50 C, response time 30min; Second time eliminating sargassum smell is carried out again, inoculum of dry yeast 1.5%, temperature 30 DEG C, fermentation time 20min with yeast;
4) Concha Ostreae enzymolysis solution secondary debitterize: to step 3) the eliminating sargassum smell enzymolysis solution that obtains adds 3% beta-schardinger dextrin-and carries out embedding debitterize, and stirred at ambient temperature 15min, fully mixes;
5) decolour: add 3% kieselguhr decolouring, control temperature 70 DEG C, desolventing technology 1h, filters;
6) concentrated: by step 5) filtrate that obtains adopts rotary evaporation in vacuo to concentrate, and be concentrated into and be about 20% to moisture, product is faint yellow paste;
7) allocate: Chinese medicine extraction liquid is obtained by following steps: by the Herba Epimedii 5 parts of following weight portion, the Radix Astragali 8 parts, Radix Codonopsis 3 parts, Fructus Corni 8 parts, Radix Rehmanniae Preparata 1 part, Fructus Lycii 8 parts, Radix Polygalae 5 parts, Rhizoma Alismatis 5 parts, 3 parts, Radix Glycyrrhizae, 5 parts, Poria, after mixing, add and be equivalent to raw material of Chinese medicine weight 8-10 pure water reflux, extract, doubly, each 1 hour, extract twice, merge extractive liquid, microwave radiation extraction liquid 10min, power 500W, carries out cryopreservation 6-8h, temperature 0-4 DEG C at once, filter, centrifugal, 3500r/min, 10min, obtain supernatant;
By step 6) concentrated solution that obtains mixes by 15:85 with Chinese medicine extraction liquid, stir, add the mixing browning inhibitor of Folium Bambosae flavone 0.08%, neutral sodium phytate 0.05%, xanthan gum 0.2%, pectin 0.2% composition, add 2% again by glucose: oligofructose: xylitol is counted 2:3:1 by weight and is mixed to get composite flavor, mixing preparation, filtration, subpackage, sterilizing obtain finished product.
Embodiment 2
A Concha Ostreae activity extract preparation method for spermatogenesis the kidney invigorating, raising sexual function, comprises the steps:
1) Concha Ostreae debitterize process: fresh Concha Ostreae is put into 1 times of weight 0.5%Na
2cO
3solution, soaks 15min, filters, and removes Na
2cO
3solution, adds 2 times of weight pure water, the Concha Ostreae software part repeatedly after washing and filtering;
2) homogenate, enzymolysis: to step 1) add the pure water of 2 times of weight in the Concha Ostreae software that obtains, homogenate is pounded with tissue mashing machine, regulate pH7, add papain enzymolysis, add papain 1000U/g by weight, hydrolysis temperature 50 DEG C, enzymolysis time 4h, be heated to 100 DEG C, enzyme denaturing 10min alive; Repeat above enzymolysis step 3 times, merge enzymolysis solution, cooling, centrifugal, 3500r/min, 10min;
3) Concha Ostreae enzymolysis solution eliminating sargassum smell: first with active carbon to step 2) enzymolysis solution that obtains carries out first time eliminating sargassum smell, active carbon addition is 2.0%, temperature 50 C, response time 30min; Second time eliminating sargassum smell is carried out again, inoculum of dry yeast 1.5%, temperature 30 DEG C, fermentation time 20min with yeast;
4) Concha Ostreae enzymolysis solution secondary debitterize: to step 3) the eliminating sargassum smell enzymolysis solution that obtains adds 3% beta-schardinger dextrin-and carries out embedding debitterize, and stirred at ambient temperature 15min, fully mixes;
5) decolour: add 3% kieselguhr decolouring, control temperature 70 DEG C, desolventing technology 2h, filters;
6) concentrated: by step 5) filtrate that obtains adopts rotary evaporation in vacuo to concentrate, and be concentrated into and be about 20% to moisture, product is faint yellow paste;
7) allocate: Chinese medicine extraction liquid is obtained by following steps: by the Herba Epimedii 10 parts of following weight portion, the Radix Astragali 3 parts, Radix Codonopsis 5 parts, Fructus Corni 3 parts, Radix Rehmanniae Preparata 3 parts, Fructus Lycii 3 parts, Radix Polygalae 8 parts, Rhizoma Alismatis 3 parts, 8 parts, Radix Glycyrrhizae, 1 part, Poria, after mixing, add and be equivalent to raw material of Chinese medicine weight 8-10 pure water reflux, extract, doubly, each 1 hour, extract twice, merge extractive liquid, microwave radiation extraction liquid 10min, power 500W, carries out cryopreservation 6-8h, temperature 0-4 DEG C at once, filter, centrifugal, 3500r/min, 10min, obtain supernatant;
By step 6) concentrated solution that obtains mixes by 20:80 with Chinese medicine extraction liquid, stir, add the mixing browning inhibitor of Folium Bambosae flavone 0.08%, neutral sodium phytate 0.05%, xanthan gum 0.2%, pectin 0.2% composition, add 5% again by glucose: oligofructose: xylitol is counted 2:3:1 by weight and is mixed to get composite flavor, mixing preparation, filtration, subpackage, sterilizing obtain finished product.
Experimental example: pharmacodynamic experiment
Effect experiment 1: on the impact of Wistar rat spermatogenesis kidney tonifying
1, test drug
The Concha Ostreae active extract composition that embodiment 1 is made.
2, animal
Wistar rat, SPF level, male, body weight 200-250g.
3, experimental technique:
Select body weight at the Wistar male rat 40 of 200-220g, be divided into 5 groups at random, often organize 8, i.e. normal group, kidney-yang deficiency model group, the high, medium and low dosage group of ocean biology nutrition liquid, except normal group, make castration model for other 4 groups, the chloral hydrate (0.5mL/100g) of 5% anaesthetizes descending bilateral orchidectomy, postoperative muscle injection penicillin 40,000 U, injection 3d is with infection continuously.High, medium and low dosage respectively according to Concha Ostreae active extract composition (10.0,5.0,1.0gkg
-1) gavage, normal group, kidney-yang deficiency model group only gavage the distilled water (0.1mL/Kg) of same volume.
The mensuration of 3.1 level of serum testosterone
After rat claims quality, lumbar injection 5% chloral hydrate (0.5mL/100g), anesthetized rat, docking gets blood about 1.5mL, 2000rapmin after blood coagulation
-1, centrifugal 15min, separation of serum ,-20 DEG C of cryopreservation are for subsequent use.The Full-automatic chemiluminescence immunoassay analysis meter (DXI-800) using Bei Ke Man to produce and matched reagent thereof, with automatic lmunoassays analyzer within reagent calibration cycle and Quality Control, according to instrumentation Programmable detection rat blood serum testosterone levels.
The preclinical mensuration of 3.2 erection
1h after last 1 administration, is placed in rat penis position (electrode is put in external orifice of urethra, and another electrode is placed in radix penis part), dips in the moistening rat radix penis part of normal saline, to strengthen its electric conductivity with cotton swab by the stimulating electrode of electric stimulating instrument.Give local electrical stimulation, voltage 6V, frequency 20Hz, the wide 5ms of ripple, start to erection time (erection incubation period) from stimulation with stopwatch record.
4, date processing
Experimental data represents with (X ± s), and data statistics SPSS11.0 software carries out, and between group, contrast adopts one way anova to carry out.
Note: * * is P<0.01, * P<0.05 compared with model group compared with normal group
5, result and discussion
Castrated rats animal pattern experimental result shows, with Normal group ratio, model group rats eclipse period of penile erection significant prolongation illustrates modeling success, and each dosage group of Concha Ostreae active extract composition all can improve Ovariectomized Rat Serum testosterone levels to some extent, compare with castration model group and can significantly shorten castrated rats eclipse period of penile erection, improve castrated rats penis to the irritability of outside stimulus, show that Concha Ostreae active extract composition of the present invention has spermatogenesis kidney tonifying effect.
Effect experiment 2: on the impact of male rat sexual function
1, test specimen
The Concha Ostreae active extract composition that embodiment 1 is made.
2, animal
Wistar rat, SPF level, male and female half and half, body weight 180-250g
3, method
Prepare female Wistar rats (180 ± 20g): only test first 48 hours subcutaneous injection estradiol benzoate injection 20 μ g/, before 4 hours again subcutaneous injection progesterone injection 500 μ g/ only, to promote the sexual maturity.
Another preparation male Wistar rat 48, be divided into 5 groups at random by number, be set to blank group (distilled water), positive controls, the embodiment of the present invention 1,2 make the high, medium and low dosage group of Concha Ostreae active extract composition (10.0,5.0,1.0gkg
-1), gastric infusion, daily 1 time, successive administration 10 days.Male rat was put into separately cage 5 minutes in after each group of last administration 24 hours, make it shake down, then a female Mus put into by every cage, starts to record following index: (1), to be fed into male Mus first time to flutter the time of catching female Mus and catch incubation period for flutterring from female Mus; (2) from female Mus be fed into male Mus occur first time ejaculation time be copulation incubation period; (3) record male Mus in 20 minutes to flutter and catch female Mus number of times and ejaculation frequency; (4) calculating is respectively organized in 20 minutes and is flutterred the number of animals of catching and ejaculating, and flutters the rate of catching and ejaculation rate.
4, result and discussion
The each dosage group of Concha Ostreae active extract composition is all improved to some extent the effect of male rat sexual function, flutters to catch incubation period and copulation is obviously shortened incubation period, flutters to catch number of times and ejaculation frequency increases.Show that Concha Ostreae active extract composition of the present invention can improve the high effect of performance.
The test of pesticide effectiveness 3: toxicity test
1, test specimen
The Concha Ostreae active extract composition that embodiment 1,2 is made.
2, animal
Kunming mouse, male and female half and half, body weight is 18-22g.
3, experimental technique
Get the embodiment of the present invention 1,2 prepare Concha Ostreae active extract composition to mice by 1.0mL/100g body weight, every day 2 times, observe successive administration 15 weeks, the indexs such as drug withdrawal 3 weeks rear mouse hair, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat.
4, result and discussion
Result shows: the index such as hair, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat of Concha Ostreae active extract composition of the present invention to mice all has no significant effect, internal organs naked eyes do not find that difference change and histological indications show, successive administration 15 weeks, drug withdrawal is after 3 weeks, and Organs of Mice is all without obviously changing.Show that Concha Ostreae active extract composition of the present invention is little to toxicity after mice long-term prescription, also there is no difference reaction after drug withdrawal, safety.
Claims (8)
1. a Concha Ostreae activity extract preparation method for spermatogenesis the kidney invigorating, raising sexual function, is characterized in that, comprise the steps:
1) Concha Ostreae debitterize process: add 1 times of weight 0.5%Na to fresh Concha Ostreae software
2cO
3solution, soaks, and filters, with 2-3 times of weight pure water, and the Concha Ostreae software part repeatedly after washing and filtering;
2) homogenate, enzymolysis: to step 1) add the pure water of 2 times of weight in the Concha Ostreae software that obtains, make homogenate, regulate pH6-7, add papain enzymolysis, enzymolysis terminates, enzyme denaturing; Repeat above enzymolysis step 2-3 time, merge enzymolysis solution, cooling, centrifugal;
3) Concha Ostreae enzymolysis solution eliminating sargassum smell: first with active carbon to step 2) enzymolysis solution that obtains carries out first time eliminating sargassum smell, then carries out second time eliminating sargassum smell with yeast;
4) Concha Ostreae enzymolysis solution secondary debitterize: to step 3) the eliminating sargassum smell enzymolysis solution that obtains adds beta-schardinger dextrin-and carries out embedding debitterize;
5) decolour: add kieselguhr decolouring, filter;
6) concentrated: by step 5) filtrate that obtains concentrates;
7) allocate: by step 6) concentrated solution that obtains is mixed in proportion with Chinese medicine extraction liquid, stirs, and add and mix browning inhibitor, composite flavor, mixing preparation, filtration, subpackage, sterilizing obtain finished product;
Described Chinese medicine extraction liquid is obtained by following steps: by Herba Epimedii 5-10 part of following weight portion, Radix Astragali 3-8 part, Radix Codonopsis 3-5 part, Fructus Corni 3-8 part, Radix Rehmanniae Preparata 1-3 part, Fructus Lycii 3-8 part, Radix Polygalae 5-8 part, Rhizoma Alismatis 3-5 part, Radix Glycyrrhizae 3-8 part, Poria 1-5 part, after mixing, add and be equivalent to raw material of Chinese medicine weight 8-10 pure water reflux, extract, doubly, each 1 hour, extract twice, merge extractive liquid, microwave radiation extraction liquid 10min, power 500W, carry out cryopreservation 6-8h, temperature 0-4 DEG C at once, filter, centrifugal, obtain supernatant;
Described mixing browning inhibitor is Folium Bambosae flavone 0.08%, neutral sodium phytate 0.05%, xanthan gum 0.2%, pectin 0.2%;
Described composite flavor is by glucose: oligofructose: xylitol is counted 2:3:1 by weight and is mixed to get, and addition is 2-5%.
2. the Concha Ostreae activity extract preparation method of a kind of spermatogenesis the kidney invigorating according to claim 1, raising sexual function, it is characterized in that: step 2) described in enzymolysis, the amount adding papain is by weight 800-1000U/g, hydrolysis temperature 50 DEG C, enzymolysis time 4h.
3. a kind of spermatogenesis the kidney invigorating according to claim 1, improve the Concha Ostreae activity extract preparation method of sexual function, it is characterized in that: step 3) described in eliminating sargassum smell, active carbon addition is 2.0%, temperature 50 C, response time 30min; Yeast eliminating sargassum smell, yeast powder addition 1.5%, temperature 30 DEG C, fermentation time 20min.
4. a kind of spermatogenesis the kidney invigorating according to claim 1, improve the Concha Ostreae activity extract preparation method of sexual function, it is characterized in that: step 4) described in secondary debitterize, add beta-schardinger dextrin-3%, stirred at ambient temperature 15min, fully mixes.
5. a kind of spermatogenesis the kidney invigorating according to claim 1, improve the Concha Ostreae activity extract preparation method of sexual function, it is characterized in that: step 5) described in kieselguhr decolouring, kieselguhr addition 3%, control temperature 70 DEG C, process 1-2h.
6. a kind of spermatogenesis the kidney invigorating according to claim 1, improve the Concha Ostreae activity extract preparation method of sexual function, it is characterized in that: step 6) described in concentrated, being concentrated into moisture is 20%, and product is faint yellow paste.
7. a kind of spermatogenesis the kidney invigorating according to claim 1, improve the Concha Ostreae activity extract preparation method of sexual function, it is characterized in that: step 7) described in concentrated solution and Chinese medicine extraction liquid be mixed in proportion, mix according to the weight ratio of 15-20:80-85.
8. the Concha Ostreae active extract composition that obtains of method according to any one of claim 1-7.
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| CN105533740A (en) * | 2015-12-24 | 2016-05-04 | 广西北部湾海皇生物科技有限公司 | Sea cucumber-traditional Chinese medicine extract with kidney-invigorating and essence-replenishing functions as well as preparation technology thereof |
| CN106579429A (en) * | 2016-12-09 | 2017-04-26 | 广西北部湾海皇生物科技有限公司 | Edible bird's nest oyster traditional Chinese medicine composite extractive for nourishing lung and kidney yin, and preparation method thereof |
| CN106579409A (en) * | 2016-12-09 | 2017-04-26 | 广西北部湾海皇生物科技有限公司 | Bird's nest, oyster and traditional Chinese medicine composite extract with functions of clearing away lung heat and engendering body fluids and preparation process of composite extract |
| CN106722993A (en) * | 2016-12-23 | 2017-05-31 | 浙江新维士生物科技有限公司 | A kind of functional food composite and oral products and preparation method thereof and their application |
| CN107259586A (en) * | 2017-08-03 | 2017-10-20 | 江西美庐乳业集团有限公司 | It is a kind of to be suitable to continue the nutritional supplementation powder that weight intense physical exercises crowd eats |
| CN108354101A (en) * | 2018-02-08 | 2018-08-03 | 舟山海研食品科技有限公司 | Oyster fermented beverage and preparation method thereof |
| CN109480255A (en) * | 2018-12-14 | 2019-03-19 | 中华全国供销合作总社昆明食用菌研究所 | De- bitter edible mushroom enzymolysis liquid of one kind and preparation method thereof |
| CN110819677A (en) * | 2019-11-29 | 2020-02-21 | 鲁东大学 | Preparation method of debitterized antioxidant oyster peptide |
| CN110841022A (en) * | 2019-12-09 | 2020-02-28 | 颜振清 | Medicinal and edible health-preserving composition for maintaining prostate and improving sexual function and preparation method thereof |
| CN111109364A (en) * | 2020-01-03 | 2020-05-08 | 中国海洋大学 | Preparation method of novel oyster goat yogurt |
| CN111109552A (en) * | 2020-01-03 | 2020-05-08 | 中国海洋大学 | Preparation method of oyster full nutrition powder capable of removing fishy smell and bitter taste |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533740A (en) * | 2015-12-24 | 2016-05-04 | 广西北部湾海皇生物科技有限公司 | Sea cucumber-traditional Chinese medicine extract with kidney-invigorating and essence-replenishing functions as well as preparation technology thereof |
| CN106579429A (en) * | 2016-12-09 | 2017-04-26 | 广西北部湾海皇生物科技有限公司 | Edible bird's nest oyster traditional Chinese medicine composite extractive for nourishing lung and kidney yin, and preparation method thereof |
| CN106579409A (en) * | 2016-12-09 | 2017-04-26 | 广西北部湾海皇生物科技有限公司 | Bird's nest, oyster and traditional Chinese medicine composite extract with functions of clearing away lung heat and engendering body fluids and preparation process of composite extract |
| CN106722993A (en) * | 2016-12-23 | 2017-05-31 | 浙江新维士生物科技有限公司 | A kind of functional food composite and oral products and preparation method thereof and their application |
| CN107259586A (en) * | 2017-08-03 | 2017-10-20 | 江西美庐乳业集团有限公司 | It is a kind of to be suitable to continue the nutritional supplementation powder that weight intense physical exercises crowd eats |
| CN108354101A (en) * | 2018-02-08 | 2018-08-03 | 舟山海研食品科技有限公司 | Oyster fermented beverage and preparation method thereof |
| CN109480255A (en) * | 2018-12-14 | 2019-03-19 | 中华全国供销合作总社昆明食用菌研究所 | De- bitter edible mushroom enzymolysis liquid of one kind and preparation method thereof |
| CN110819677A (en) * | 2019-11-29 | 2020-02-21 | 鲁东大学 | Preparation method of debitterized antioxidant oyster peptide |
| CN110841022A (en) * | 2019-12-09 | 2020-02-28 | 颜振清 | Medicinal and edible health-preserving composition for maintaining prostate and improving sexual function and preparation method thereof |
| CN111109364A (en) * | 2020-01-03 | 2020-05-08 | 中国海洋大学 | Preparation method of novel oyster goat yogurt |
| CN111109552A (en) * | 2020-01-03 | 2020-05-08 | 中国海洋大学 | Preparation method of oyster full nutrition powder capable of removing fishy smell and bitter taste |
| CN111109364B (en) * | 2020-01-03 | 2022-04-05 | 中国海洋大学 | Preparation method of novel oyster goat yogurt |
| CN111109552B (en) * | 2020-01-03 | 2022-06-21 | 中国海洋大学 | Preparation method of oyster full nutrition powder capable of removing fishy smell and bitter taste |
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