CN102551043A - Oyster polypeptide nutrition supplement food for children and preparation method thereof - Google Patents
Oyster polypeptide nutrition supplement food for children and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an oyster polypeptide nutrition supplement food for children and a preparation method thereof. The method comprises the following steps: deodorizing, debittering and decoloring enzymolysis polypeptide fluid which is prepared by controlled enzymatic hydrolysis of oyster meat; carrying out suction filtering, concentrating and drying to obtain oyster peptide powder; culturing and centrifuging probiotics suitable for children to obtain bacterial paste; lyophilizing with cryoprotectant to obtain probiotics lyophilized bacterial powder; preparing probiotic microcapsules by the probiotics lyophilized bacterial powder and a wall material; forming prebiotics by selecting fructo-oligosaccharide and isomaltose hypgather; weighing the following components in percentage by weight: 40 to 60 percent of oyster peptide powder, 20 to 30 percent of the probiotic microcapsules and 10 to 40 percent of prebiotics; and uniformly stirring, packaging in bags to obtain the oyster polypeptide nutrition supplement food. The oyster polypeptide nutrition supplement food quickly increases the number of beneficial bacteria in intestinal canals of children while providing rich nutrition to children, so that the environment in a body is improved, and the nutrition absorption capability is improved. The preparation method has the advantages of reasonable process, simple manufacture and strong operability.
Description
Technical field
The present invention relates to a kind of food compositions or its processing, especially seed oyster polypeptide child nutrition supplement and preparation method thereof that contains the peptide class.
Background technology
Children are special colonies, and its particularity just shows that they are in body, brain growth phase.Therefore; Sufficient, the rational nutrition of allotment is the material base of upgrowth and development of children; If subalimentation then at first cause children's body weight not increase even descend finally also can influence the growth of height and the function of other each system of health, like immunologic function, endocrine function, dysfunction of nervous regulation etc.Because children are in the puberty, its intestines, stomach function are sound as yet, and the child nutrition food of being sold on the market of being arranged in pairs or groups by cereal or fruits and vegetables class and fish, meat is for children, and ubiquity non-digestible deficiency.
In order to improve children's digestive system, optimize the gut flora balance, enhance immunity power, the absorption of help nutriment, numerous technical staff have invented the children's health food that has prebiotics or probio.For example: on November 17th, the 2004 disclosed CN1545937A Chinese invention patent ublic specification of application disclosed " a kind of children's health food that contains prebiotics and preparation method thereof ", this health food comprises following composition: prebiotics 5～80%, amino acid 5～30%, trace element 0.1～3%, food additives 2～89.9%.It has to improve and grows, to promote the function that digests.For another example; In on August 15th, the 2007 disclosed CN101015570A Chinese invention patent ublic specification of application disclosed " children's health food of probiotics and production technology thereof ", it comprises following composition: bifidobacterium infantis, lactobacillus acidophilus, saccharomyces cerevisiae, yeast zinc, vitamin C, vitamin B1, vitamin B2, vitamin B6, cobalamin, niacinamide, calcium pantothenate, oligomeric FOS, glucose, 2% Gonak.It stimulates immune cell with bacterium system bacterium, builds up resistance, and reduces enteron aisle pH value, helps the absorption of calcium, phosphorus, zinc, ferro element, thereby improves symptoms such as diarrhoea, vomiting, abdominal distension, dysentery, constipation, apocleisis, indigestion, anaemia, calcium deficiency rapidly.Above-mentioned disclosed children's health food only has the regulating system immunologic function, improves the gut flora balance, promotes useful physiological functions such as absorption of nutrient ingredients, but the required nutriment of upgrowth and development of children is not played supplementary function.
We know, fertile smooth, the delicious flavour, nutritious of oyster meat have the laudatory title of " seabed milk ".Modern nutriology research shows: oyster meat is rich in good protein, glycogen and vitamin A, B
1, B
2, the multiple trace element that human physiological functions is had important function such as D, zinc, calcium, phosphorus, copper, iodine, manganese, barium.Particularly: the good protein in the oyster meat comprises the amino acid of needed by human, and amino acid is formed perfect, surpasses cow's milk and human milk; Also contain abundant natural taurine in the oyster meat; The physiological function of taurine relates to retina, central nervous system, cardiovascular system, liver and gall, internal system etc.; And multiple metabolic process in the participation human body, have effects such as children's brain development of promotion and mind-tranquilizing brain-strengthening; The content of zinc occupies first of the human foods in the oyster meat, and zinc is participated in nucleic acid, enzyme etc. and synthesized, has the effect that promotes body development, enhances human body resistance, and children lack zinc can cause growth retardation; Fat content in the oyster meat is less, but aliphatic acid form in content of polyunsaturated fatty acid abundant, mainly be EPA and DHA, it can effectively promote brain cell and neural growth, enhance memory power and thinking ability, raising IQ, the eyesight of improving.Therefore, oyster meat can be used as the first-selected wholefood of upgrowth and development of children.
At present, also do not see the report of oyster polypeptide powder that the oyster meat processed obtains and prebiotics, the synthetic child nutrition supplement of probiotic group.
Summary of the invention
In order to overcome the prior art children's health food only produces useful physiological action to the host deficiency, the present invention provides a kind of reasonable, multiple functional, that production technology is advanced oyster polypeptide child nutrition supplement and preparation method thereof of forming.
The technical solution adopted for the present invention to solve the technical problems is: a seed oyster polypeptide child nutrition supplement; It comprises probiotics preparation and prebiotics; It is characterized in that: it also includes the oyster peptide powder, and the percentage by weight of its each component is respectively: oyster peptide powder 40-60%, probiotics preparation 20-30%, prebiotics 10-40%.
Described oyster peptide powder is oyster albumen control enzymolysis, and the enzymolysis product that obtains mixed polypeptide is through the resulting peptide powder of drying.
Described probiotics preparation is following one or more the microencapsulated formulation of probio: bifidobacterium infantis, bifidobacterium bifidum, lactobacillus acidophilus, bifidobacterium adolescentis, streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium longum, bifidobacterium breve.
Described prebiotics is that FOS and oligoisomaltose combine in 1: 1～1: 3 ratio of mass ratio.
The preparation method of one seed oyster polypeptide child nutrition supplement is characterized in that: process the following step:
1., the preparation of oyster peptide powder
It is raw material that fresh oyster meat is chosen in the processing of a, raw material, sends into tissue after cleaning and smashs homogenate in the equipment to pieces;
The water that oyster meat after the homogenate that b, enzymolysis obtain step a adds 2～4 times of its weight dilutes; Regulating the pH value is 6.0～8.0; Add in trypsase, papain, compound protease, the flavor protease one or more then, its enzyme dosage is: every gram oyster albumen adds 2000～4000 enzymes units (u) that live, 45～60 ℃ of control temperature; Enzymolysis 2～4h gets oyster proteolysis polypeptide liquid;
The oyster proteolysis polypeptide liquid that obtains is carried out boiling water heating bath 10～20min, and the enzyme that goes out is handled, the oyster proteolysis polypeptide liquid of the enzyme that must go out;
C, take off raw meat, debitterize, decolouring and handle
The oyster proteolysis polypeptide liquid of the enzyme that goes out that step b is obtained adds the dusty yeast of its weight 0.3～0.8%, under 30～50 ℃ of temperature conditions, stirs, and leaves standstill 1～2h again, takes off raw meat and handles;
To take off the cycloheptaamylose that oyster proteolysis polypeptide liquid after the raw meat adds its weight 0.5～1%, under 45～55 ℃ of conditions, evenly stir 5～10min, and carry out debitterize and handle;
With the diatomite and the active carbon of its weight 1～3% of the adding of the oyster proteolysis polypeptide liquid behind the debitterize, under 50～70 ℃ condition, decolour and handle 1～2h, wherein the weight ratio of diatomite and active carbon is 2: 3;
D, suction filtration with step c obtain take off raw meat, debitterize, decolouring and handle after oyster proteolysis polypeptide liquid suction filtration in vacuum filtration equipment, obtain oyster polypeptide liquid transparent, free from extraneous odour;
E, concentrate: steps d is obtained transparent, free from extraneous odour oyster polypeptide liquid vacuum simmer down to original volume 1/5～1/6, oyster polypeptide concentrate;
F, drying: the oyster polypeptide concentrate that step e is obtained carries out spray-drying, obtains the oyster peptide powder, wherein the drying process with atomizing condition: the spray-drying EAT is 170 ℃～190 ℃, leaving air temp: 60 ℃～80 ℃;
2., the preparation of probiotics preparation
One or more the probio that the preparation of g, probio freeze-dried powder is chosen in bifidobacterium infantis, bifidobacterium bifidum, lactobacillus acidophilus, bifidobacterium adolescentis, streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium longum, the bifidobacterium breve is a bacterial classification; In culture medium, cultivate; The bacterial classification inoculation amount is 6～8%; 35～45 ℃ of cultivation temperature, initial pH value 6.0～8.0, frequency of oscillation 120～250r/min; Replenish glucose with the proper flow acceleration after cultivating 10～24h, continue to be cultured to results; Zymotic fluid with the centrifugal 5～15min of the rotating speed of 2000～4000r/min, obtains bacterium mud in 4 ℃ of centrifuges, in bacterium mud, add then to carry out the freeze-drying processing after freeze drying protectant stirs, and obtains probio freeze-dried vaccine powder;
The preparation of h, probiotic microcapsule: choose sodium alginate, gelatin and Arabic gum as the wall material, choose probio freeze-dried vaccine powder that step g obtains, core and wall material are prepared probiotic microcapsule by a certain percentage as core;
3., prebiotics chooses
Choose FOS and oligoisomaltose and be combined into prebiotics in 1: 1～1: 3 ratio of mass ratio;
4., allocate weighing by weight percentage: the oyster peptide powder 40～60% that 1. step prepares, the probiotic microcapsule 20～30% that 2. step prepares, the prebiotics 10～40% that 3. step is chosen, stir, obtain oyster polypeptide probiotic powder;
5., the packing by conventional weight the above-mentioned oyster polypeptide probiotic powder that obtains is carried out bag distribution packaging, get product.
Freeze drying protectant in the said adding bacterium mud is made up of following component: sucrose, trehalose, skimmed milk and L-sodium glutamate, its addition are respectively 6～14%, 0.5～2.0%, 4～12%, 0.5～2.0% of bacterium mud weight.
Sodium alginate, gelatin, Arabic gum in the said wall material is according to mass ratio 1～3: 1: 1 ratio combines.
The ratio of the core of said making probiotic microcapsule and wall material is volume ratio 1: 1.
The present invention is according to the upgrowth and development of children nutritional need, as major ingredient, adds the child nutrition supplement that probiotics preparation and prebiotics prepare with the oyster peptide powder.Wherein the oyster peptide powder is to adopt the modern food biotechnology with oyster albumen control enzymolysis, obtains to have than amino acid the mixed polypeptide enzymolysis product of many physiological functions such as better digestion and absorption function, antiallergy, enhance immunity, gets through spray-drying; Probiotics preparation is the probio that preferably is fit to children, uses microcapsules technology and guarantees that probio keeps permanent active, strengthens the tolerance to hydrochloric acid in gastric juice and bile, and the enteron aisle and being colonizated on the intestinal mucosa of can going directly after the food plays a role; Prebiotics has unique physiological function, and can promote the probio growth.Oyster polypeptide child nutrition supplement of the present invention is when providing abundant nutrition for children, and beneficial bacterium quantity in the children's enteron aisle that increases sharply suppresses harmful bacterium and increases; Recover the gut flora balance, improve internal milieu, improve the absorbability of child nutrition (such as trace elements such as protein, various vitamin, calcium, zinc, iron); Children's appetite is increased; Digest and assimilate, defecation is normal, improves children self resistance comprehensively, builds up health, brain tonic and intelligence development.Preparation method's technology of this oyster polypeptide child nutrition supplement rationally, make simple, strong operability, be suitable for large-scale production.
The specific embodiment
Below in conjunction with embodiment the present invention is further specified.
Embodiment 1
The preparation method of one seed oyster polypeptide child nutrition supplement, its process the following step:
1., the preparation of oyster peptide powder
It is raw material that fresh oyster meat is chosen in the processing of a, raw material, sends into tissue after cleaning and smashs homogenate in the equipment to pieces;
The water that oyster meat after the homogenate that b, enzymolysis obtain step a adds 3 times of its weight dilutes; Regulating the pH value is 7.0, adds papain, flavor protease then, and its papain, the two enzyme dosages of flavor protease are: every gram oyster albumen adds 3000 enzymes unit (u) alive; The enzyme proportion of composing is a papain: flavor protease=1: 1; 55 ℃ of control temperature, enzymolysis 3h gets oyster proteolysis polypeptide liquid;
The oyster proteolysis polypeptide liquid that obtains is carried out boiling water heating bath 20min, and the enzyme that goes out is handled, the oyster proteolysis polypeptide liquid of the enzyme that must go out;
C, take off raw meat, debitterize, decolouring and handle
The oyster proteolysis polypeptide liquid of the enzyme that goes out that step b is obtained adds the dusty yeast of its weight 0.6%, under 50 ℃ of temperature conditions, stirs, and leaves standstill 2h again, takes off raw meat and handles;
To take off the cycloheptaamylose that oyster proteolysis polypeptide liquid after the raw meat adds its weight 0.5%, under 50 ℃ of conditions, evenly stir 8min, and carry out debitterize and handle;
With the diatomite and the active carbon of its weight 2% of the adding of the oyster proteolysis polypeptide liquid behind the debitterize, under 60 ℃ condition, decolour and handle 1.5h, wherein the weight ratio of diatomite and active carbon is 2: 3;
D, suction filtration with step c obtain take off raw meat, debitterize, decolouring and handle after oyster proteolysis polypeptide liquid suction filtration in vacuum filtration equipment, control vacuum 0.09MPa through 0.45 μ filter membrane, obtains oyster polypeptide liquid transparent, free from extraneous odour;
E, concentrate: steps d is obtained transparent, free from extraneous odour oyster polypeptide liquid vacuum simmer down to original volume 1/5, oyster polypeptide concentrate;
F, drying: the oyster polypeptide concentrate that step e is obtained carries out spray-drying, obtains the oyster peptide powder, wherein the drying process with atomizing condition: EAT is 180 ℃, and leaving air temp is 65 ℃;
2., the preparation of probiotics preparation
The preferred bifidobacterium infantis of the preparation of g, probio freeze-dried powder, bifidobacterium bifidum, lactobacillus acidophilus, bifidobacterium adolescentis are the probio kind; In culture medium, cultivate; The bacterial classification inoculation amount is 8%, 39 ℃ of cultivation temperature, initial pH value 6.0; Frequency of oscillation 160r/min cultivates behind the 12h with feed supplement speed 6.0m
3/ h replenishes glucose, continues to be cultured to results; Zymotic fluid with the centrifugal 5min of the rotating speed of 4000r/min, obtains bacterium mud in 4 ℃ of centrifuges, in bacterium mud, add then to carry out the freeze-drying processing after freeze drying protectant stirs, and obtains probio freeze-dried vaccine powder; Wherein, freeze drying protectant proportioning: add the sucrose of bacterium mud weight 12%, 1% trehalose, 8% skimmed milk, 1.5% L-sodium glutamate.
The preparation of h, probiotic microcapsule: choose sodium alginate, gelatin and Arabic gum as the wall material, wherein, the mass ratio of sodium alginate, gelatin, Arabic gum is 3: 1: 1; Choose probio freeze-dried vaccine powder that step g obtains as core, core and wall material are prepared probiotic microcapsule in 1: 1 by volume ratio;
3., prebiotics chooses
Choose commercially available FOS and oligoisomaltose and be combined into prebiotics in 1: 1 ratio of mass ratio;
4., allocate weighing by weight percentage: the oyster peptide powder 40% that 1. step prepares, the probiotic microcapsule 20% that 2. step prepares, the prebiotics 40% that 3. step is chosen; The employing mixer stirs; Wherein the rotating speed of mixer is controlled to be 200r/min; Mixing time is 20min, obtains oyster polypeptide probiotic powder;
5., the packing by 3 gram/bags the above-mentioned oyster polypeptide probiotic powder that obtains is carried out bag distribution packaging, get product.
Embodiment 2
The preparation method of one seed oyster polypeptide child nutrition supplement, its process the following step:
1., the preparation of oyster peptide powder
It is raw material that fresh oyster meat is chosen in the processing of a, raw material, sends into tissue after cleaning and smashs homogenate in the equipment to pieces;
The water that oyster meat after the homogenate that b, enzymolysis obtain step a adds 4 times of its weight dilutes; Regulating the pH value is 6.5, adds trypsase, compound protease then, and its trypsase, the two enzyme dosages of compound protease are that every gram oyster albumen adds 2000 enzymes unit (u) alive; The enzyme proportion of composing is a trypsase: compound protease=2: 1; The control temperature is 50 ℃, and enzymolysis 4h gets oyster proteolysis polypeptide liquid;
The oyster proteolysis polypeptide liquid that obtains is carried out boiling water heating bath 15min, and the enzyme that goes out is handled, the oyster proteolysis polypeptide liquid of the enzyme that must go out;
C, take off raw meat, debitterize, decolouring and handle
The oyster proteolysis polypeptide liquid of the enzyme that goes out that step b is obtained adds the dusty yeast of its weight 0.4%, under 40 ℃ of temperature conditions, stirs, and leaves standstill 1.5h again, takes off raw meat and handles;
To take off the cycloheptaamylose that oyster proteolysis polypeptide liquid after the raw meat adds its weight 0.8%, under 55 ℃ of conditions, evenly stir 5min, and carry out debitterize and handle;
With the diatomite and the active carbon of its weight 1% of the adding of the oyster proteolysis polypeptide liquid behind the debitterize, under 50 ℃ condition, decolour and handle 2h, wherein the weight ratio of diatomite and active carbon is 2: 3;
D, suction filtration with step c obtain take off raw meat, debitterize, decolouring and handle after oyster proteolysis polypeptide liquid suction filtration in vacuum filtration equipment, control vacuum 0.09MPa through 0.45 μ filter membrane, obtains oyster polypeptide liquid transparent, free from extraneous odour;
E, concentrate: steps d is obtained transparent, free from extraneous odour oyster polypeptide liquid vacuum simmer down to original volume 1/6, oyster polypeptide concentrate;
F, drying: the oyster polypeptide concentrate that step e is obtained carries out spray-drying, obtains the oyster peptide powder, wherein the drying process with atomizing condition: the spray-drying EAT is that EAT is 190 ℃, and leaving air temp is 70 ℃;
2., the preparation of probiotics preparation
The preferred bifidobacterium bifidum of the preparation of g, probio freeze-dried powder, streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium longum are the probio kind; In culture medium, cultivate; Bacterial classification inoculation amount 6%, 35 ℃ of cultivation temperature, initial pH6.5; Frequency of oscillation 140r/min cultivates behind the 15h with feed supplement speed 5.0m
3/ h replenishes glucose, continues to be cultured to results; Zymotic fluid with the centrifugal 10min of the rotating speed of 3000r/min, obtains bacterium mud in 4 ℃ of centrifuges, in bacterium mud, add then to carry out the freeze-drying processing after freeze drying protectant stirs, and obtains probio freeze-dried vaccine powder; Wherein, freeze drying protectant proportioning: add the sucrose of bacterium mud weight 10%, 1.2% trehalose, 6% skimmed milk, 2.0% L-sodium glutamate, obtain probio freeze-dried vaccine powder;
(8) probiotic microcapsule preparation
The preparation of h, probiotic microcapsule: choose sodium alginate, gelatin and Arabic gum as the wall material, wherein, the mass ratio of sodium alginate, gelatin, Arabic gum is 2: 1: 1; Choose probio freeze-dried vaccine powder that step g obtains as core, core and wall material are prepared probiotic microcapsule in 1: 1 by volume ratio;
3., prebiotics chooses
Choose FOS and oligoisomaltose and be combined into prebiotics in 1: 2 ratio of mass ratio;
4., allocate weighing by weight percentage: the oyster peptide powder 50% that 1. step prepares, the probiotic microcapsule 25% that 2. step prepares, the prebiotics 25% that 3. step is chosen; The employing mixer stirs; Wherein the rotating speed of mixer is controlled to be 150r/min; Mixing time is 30min, obtains oyster polypeptide probiotic powder;
5., the packing by 5 gram/bags the above-mentioned oyster polypeptide probiotic powder that obtains is carried out bag distribution packaging, get product.
Embodiment 3
The preparation method of one seed oyster polypeptide child nutrition supplement, its process the following step:
1., the preparation of oyster peptide powder
It is raw material that fresh oyster meat is chosen in the processing of a, raw material, sends into tissue after cleaning and smashs homogenate in the equipment to pieces;
The water that oyster meat after the homogenate that b, enzymolysis obtain step a adds 2 times of its weight dilutes, and regulating the pH value is 8.0, adds papain then; Its consumption is: every gram oyster albumen adds 4000 enzymes unit (u) alive; 45 ℃ of control temperature, enzymolysis 2h gets oyster proteolysis polypeptide liquid;
The oyster proteolysis polypeptide liquid that obtains is carried out boiling water heating bath 10min, and the enzyme that goes out is handled, the oyster proteolysis polypeptide liquid of the enzyme that must go out;
C, take off raw meat, debitterize, decolouring and handle
The oyster proteolysis polypeptide liquid of the enzyme that goes out that step b is obtained adds the dusty yeast of its weight 0.8%, under 30 ℃ of temperature conditions, stirs, and leaves standstill 1h again, takes off raw meat and handles;
To take off the cycloheptaamylose that oyster proteolysis polypeptide liquid after the raw meat adds its weight 1%, under 45 ℃ of conditions, evenly stir 10min, and carry out debitterize and handle;
With the diatomite and the active carbon of its weight 3% of the adding of the oyster proteolysis polypeptide liquid behind the debitterize, under 70 ℃ condition, decolour and handle 1h, wherein the weight ratio of diatomite and active carbon is 2: 3;
D, suction filtration with step c obtain take off raw meat, debitterize, decolouring and handle after oyster proteolysis polypeptide liquid suction filtration in vacuum filtration equipment, control vacuum 0.09MPa through 0.45 μ filter membrane, obtains oyster polypeptide liquid transparent, free from extraneous odour;
E, concentrate: steps d is obtained transparent, free from extraneous odour oyster polypeptide liquid vacuum simmer down to original volume 1/5, oyster polypeptide concentrate;
F, drying: the oyster polypeptide concentrate that step e is obtained carries out spray-drying, obtains the oyster peptide powder, wherein the drying process with atomizing condition: EAT is 170 ℃, and leaving air temp is 80 ℃;
2., the preparation of probiotics preparation
The preferred bifidobacterium infantis of the preparation of g, probio freeze-dried powder, bifidobacterium bifidum, bifidobacterium breve are the probio kind, in culture medium, cultivate, and the bacterial classification inoculation amount is 7%; 42 ℃ of cultivation temperature; Initial pH value 8.0, frequency of oscillation 220r/min cultivates behind the 24h with feed supplement speed 5.5m
3/ h replenishes glucose, continues to be cultured to results; Zymotic fluid with the centrifugal 15min of the rotating speed of 2000r/min, obtains bacterium mud in 4 ℃ of centrifuges, in bacterium mud, add then to carry out the freeze-drying processing after freeze drying protectant stirs, and obtains probio freeze-dried vaccine powder; Wherein, freeze drying protectant proportioning: add the sucrose of bacterium mud weight 6%, 2% trehalose, 12% skimmed milk, 0.5% L-sodium glutamate.
The preparation of h, probiotic microcapsule: choose sodium alginate, gelatin and Arabic gum as the wall material, wherein, the mass ratio of sodium alginate, gelatin, Arabic gum is 1: 1: 1; Choose probio freeze-dried vaccine powder that step g obtains as core, core and wall material are prepared probiotic microcapsule in 1: 1.5 by volume ratio;
3., prebiotics chooses
Choose commercially available FOS and oligoisomaltose and be combined into prebiotics in 1: 3 ratio of mass ratio;
4., allocate weighing by weight percentage: the oyster peptide powder 60% that 1. step prepares, the probiotic microcapsule 30% that 2. step prepares, the prebiotics 10% that 3. step is chosen; The employing mixer stirs; Wherein the rotating speed of mixer is controlled to be 200r/min; Mixing time is 20min, obtains oyster polypeptide probiotic powder;
5., the packing by 6 gram/bags the above-mentioned oyster polypeptide probiotic powder that obtains is carried out bag distribution packaging, get product.
Oyster peptide powder child nutrition supplement of the present invention is fit to the upgrowth and development of children nutritional need, when abundant nutrition being provided for children, and beneficial bacterium quantity in the children's enteron aisle that increases sharply; Suppressing harmful bacterium increases; Recover the gut flora balance, improve internal milieu, help to improve child nutrition (such as trace elements such as protein, various vitamin, calcium, zinc, iron) absorbability; Children's appetite is increased; Digest and assimilate, defecation is normal, improves children self resistance comprehensively, builds up health, brain tonic and intelligence development.Its preparation method technology is advanced rationally, operation feasible.
Claims (8)
1. a seed oyster polypeptide child nutrition supplement; It comprises probiotics preparation and prebiotics; It is characterized in that: it also includes the oyster peptide powder, and the percentage by weight of its each component is respectively: oyster peptide powder 40-60%, probiotics preparation 20-30%, prebiotics 10-40%.
2. a seed oyster polypeptide child nutrition supplement according to claim 1 is characterized in that: described oyster peptide powder is oyster albumen control enzymolysis, and the enzymolysis product that obtains mixed polypeptide is through the resulting peptide powder of drying.
3. a seed oyster polypeptide child nutrition supplement according to claim 1 is characterized in that: described probiotics preparation is following one or more the microencapsulated formulation of probio: bifidobacterium infantis, bifidobacterium bifidum, lactobacillus acidophilus, bifidobacterium adolescentis, streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium longum, bifidobacterium breve.
4. a seed oyster polypeptide child nutrition supplement according to claim 1 is characterized in that: described prebiotics is that FOS and oligoisomaltose combine in 1: 1～1: 3 ratio of mass ratio.
5. the preparation method of a seed oyster polypeptide child nutrition supplement is characterized in that: through the following step:
1., the preparation of oyster peptide powder
It is raw material that fresh oyster meat is chosen in the processing of a, raw material, sends into tissue after cleaning and smashs homogenate in the equipment to pieces;
The water that oyster meat after the homogenate that b, enzymolysis obtain step a adds 2～4 times of its weight dilutes; Regulating the pH value is 6.0～8.0; Add in trypsase, papain, compound protease, the flavor protease one or more then, its enzyme dosage is: every gram oyster albumen adds 2000～4000 enzymes units (u) that live, 45～60 ℃ of control temperature; Enzymolysis 2～4h gets oyster proteolysis polypeptide liquid;
The oyster proteolysis polypeptide liquid that obtains is carried out boiling water heating bath 10～20min, and the enzyme that goes out is handled, the oyster proteolysis polypeptide liquid of the enzyme that must go out;
C, take off raw meat, debitterize, decolouring and handle
The oyster proteolysis polypeptide liquid of the enzyme that goes out that step b is obtained adds the dusty yeast of its weight 0.3～0.8%, under 30～50 ℃ of temperature conditions, stirs, and leaves standstill 1～2h again, takes off raw meat and handles;
To take off the cycloheptaamylose that oyster proteolysis polypeptide liquid after the raw meat adds its weight 0.5～1%, under 45～55 ℃ of conditions, evenly stir 5～10min, and carry out debitterize and handle;
With the diatomite and the active carbon of its weight 1～3% of the adding of the oyster proteolysis polypeptide liquid behind the debitterize, under 50～70 ℃ condition, decolour and handle 1～2h, wherein the weight ratio of diatomite and active carbon is 2: 3;
D, suction filtration with step c obtain take off raw meat, debitterize, decolouring and handle after oyster proteolysis polypeptide liquid suction filtration in vacuum filtration equipment, obtain oyster polypeptide liquid transparent, free from extraneous odour;
E, concentrate: steps d is obtained transparent, free from extraneous odour oyster polypeptide liquid vacuum simmer down to original volume 1/5～1/6, oyster polypeptide concentrate;
F, drying: the oyster polypeptide concentrate that step e is obtained carries out spray-drying, obtains the oyster peptide powder, wherein the drying process with atomizing condition: the spray-drying EAT is 170 ℃～190 ℃, leaving air temp: 60 ℃～80 ℃;
2., the preparation of probiotics preparation
One or more the probio that the preparation of g, probio freeze-dried powder is chosen in bifidobacterium infantis, bifidobacterium bifidum, lactobacillus acidophilus, bifidobacterium adolescentis, streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium longum, the bifidobacterium breve is a bacterial classification; In culture medium, cultivate; The bacterial classification inoculation amount is 6～8%; 35～45 ℃ of cultivation temperature, initial pH value 6.0～8.0, frequency of oscillation 120～250r/min; Replenish glucose with the proper flow acceleration after cultivating 10～24h, continue to be cultured to results; Zymotic fluid with the centrifugal 5～15min of the rotating speed of 2000～4000r/min, obtains bacterium mud in 4 ℃ of centrifuges, in bacterium mud, add then to carry out the freeze-drying processing after freeze drying protectant stirs, and obtains probio freeze-dried vaccine powder;
The preparation of h, probiotic microcapsule: choose sodium alginate, gelatin and Arabic gum as the wall material, choose probio freeze-dried vaccine powder that step g obtains, core and wall material are prepared probiotic microcapsule by a certain percentage as core;
3., prebiotics chooses
Choose FOS and oligoisomaltose and be combined into prebiotics in 1: 1～1: 3 ratio of mass ratio;
4., allocate weighing by weight percentage: the oyster peptide powder 40～60% that 1. step prepares, the probiotic microcapsule 20～30% that 2. step prepares, the prebiotics 10～40% that 3. step is chosen, stir, obtain oyster polypeptide probiotic powder;
5., the packing by conventional weight the above-mentioned oyster polypeptide probiotic powder that obtains is carried out bag distribution packaging, get product.
6. the preparation method of a seed oyster polypeptide child nutrition supplement according to claim 5; It is characterized in that: the freeze drying protectant in the said adding bacterium mud is made up of following component: sucrose, trehalose, skimmed milk and L-sodium glutamate, its addition are respectively 6～14%, 0.5～2.0%, 4～12%, 0.5～2.0% of bacterium mud weight.
7. the preparation method of a seed oyster polypeptide child nutrition supplement according to claim 5 is characterized in that: sodium alginate, gelatin, the Arabic gum in the said wall material is according to mass ratio 1～3: 1: 1 ratio combines.
8. the preparation method of a seed oyster polypeptide child nutrition supplement according to claim 5 is characterized in that: the ratio of the core of said making probiotic microcapsule and wall material is volume ratio 1: 1.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554286A (en) * | 2003-12-26 | 2004-12-15 | 江南大学 | Compreheusive development and use of oyster |
| CN101496575A (en) * | 2008-01-28 | 2009-08-05 | 中国食品发酵工业研究院 | Special nutrient foodstuffs formula containing small peptide and probiotics |
| CN101971920A (en) * | 2010-09-28 | 2011-02-16 | 北京市农林科学院 | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof |
| CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
-
2011
- 2011-10-29 CN CN2011103459805A patent/CN102551043B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554286A (en) * | 2003-12-26 | 2004-12-15 | 江南大学 | Compreheusive development and use of oyster |
| CN101496575A (en) * | 2008-01-28 | 2009-08-05 | 中国食品发酵工业研究院 | Special nutrient foodstuffs formula containing small peptide and probiotics |
| CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
| CN101971920A (en) * | 2010-09-28 | 2011-02-16 | 北京市农林科学院 | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof |
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