CN104328076A - Preparation method of lactobacillus paracasei probiotics tablet of bifidobacteria producing bile salt hydrolase - Google Patents

Preparation method of lactobacillus paracasei probiotics tablet of bifidobacteria producing bile salt hydrolase Download PDF

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CN104328076A
CN104328076A CN201410640001.2A CN201410640001A CN104328076A CN 104328076 A CN104328076 A CN 104328076A CN 201410640001 A CN201410640001 A CN 201410640001A CN 104328076 A CN104328076 A CN 104328076A
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probiotics
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tablet
freeze
lactobacillus paraceasi
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刘慧�
张红星
熊利霞
张晶
高秀芝
金君华
谢远红
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Beijing University of Agriculture
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Abstract

The invention relates to bifidobacteria producing bile salt hydrolase and a preparation method of a probiotics tablet which has the function of reducing serum cholesterol, and the method is suitable for producing microecological preparations such as functional probiotics lactic acid bacteria living bacteria. The formula of the probiotics tablet provided by the invention comprises lactobacillus paracasei KL1-Liu probiotics powder and auxiliary materials such as skim milk powder, fructo-oligose, xylitol, cane sugar, citric acid, microcrystalline cellulose and maltodextrin. The preparation method comprises the following steps: performing processes such as activation, enlarged cultivation, high-density fermentation, concentration by centrifuging, freeze-drying protective additive addition, pre-freezing and freezing on lactobacillus paracasei KL1-Liu CGMCC No. 7029 of the bifidobacteria producing bile salt hydrolase, thereby preparing the probiotics powder; and mixing the crushed and sieved auxiliary materials with the probiotics powder and compressing to prepare the probiotics tablet. The probiotics tablet provided by the invention has good storage stability and the expiration date of living bacteria at 4 DEG C is at least over 1 year, so that the tablet still has the health function of reducing serum cholesterol. According to the probiotics tablet prepared by the invention, the raw materials are easily available, the process is simple, the preparation period is short and the operation is simple. The tablet is suitable for industrial production.

Description

A kind of preparation method producing bile salt hydrolase lactobacillus paraceasi probiotics tablets
Technical field
The present invention relates to and a kind ofly produce bile salt hydrolase and there is the preparation method of the probiotics tablets reducing serum cholesterol effect, be applicable to the production of the probioticses such as functional probiotic lactobacillus active bacteria formulation (tablet form, powder-type).
Background technology
Lactobacillus paraceasi (Lactobacillus paracasei) KL1-Liu that the present invention relates to be from Kefir grain separation screening obtain a kind ofly produce bile salt hydrolase and there is the probiotic lactobacillus reducing the effect such as serum cholesterol effect and regulating intestinal canal colony balance.
Probiotic bacterium (Probiotics) refers to that a class is improved host's privileged site microecological balance by enteron aisle colonized action and has the microorganism of other beneficial physiological functions some concurrently.They are by improving the enteric microorganism eubiosis, control intestinal tract infections, reduce serum cholesterol level, improve lactose intolerance people to the utilization of lactose, and can stimulate specificity or nonspecific immune reaction, have the effect of immunostimulant.The viable bacteria of probiotic composition should metabolic stability, and activity is comparatively strong, by large number of viable after digestive tube, plays probiotic action after entering enteron aisle.The current excellent species for the production of probiotic bacterium series products has the Lactococcus lactis subsp.lactis etc. in genus bifidobacterium, lactobacillus, lactococcus.
The present invention lactobacillus paraceasi KL1-Liu used utilizes to select culture technique from Kefir grain, screen lactobacillus paraceasi (Lactobacillus paracasei) KL1-Liu obtaining high yield bile salt hydrolase and exocellular polysaccharide.Research finds, this bacterial strain has tolerance gi tract inverse ring border (hydrochloric acid in gastric juice and cholate) ability more by force, surely can grow and play prebiotic effect in enteron aisle; Animal experiment study shows, and the bile salt hydrolase that this bacterial strain produces can significantly reduce or regulate serum cholesterol level, and its exocellular polysaccharide produced has the proliferation function significantly suppressing human colon cancer cell; Animal experiment study shows, and this bacterial strain also has regulating intestinal canal colony balance, preventing constipation, anti peroxidation of lipid and protects the functions such as liver.The mechanism that seminar's systematic study discloses this bacterial strain reduction serum cholesterol is the bile salt hydrolase (Bile Salt Hydrolase, BSH) producing a large amount of high vigor because of lactobacillus paraceasi KL1-Liu metabolism.The meta-bolites that BSH is genus bifidobacterium in enteron aisle, multiple lactic-acid-bacterium in lactobacillus, lactococcus produces in process of growth.Experimental results demonstrate, bile salt hydrolase will can decompose in conjunction with cholate in hepato-enteric circulation, produces amino acid and the lower free cholate of solubleness.The latter can be combined with cholesterol and forms sediment composite and excrete, thus reduces the content of serum total cholesterol.This bacterial strain full-length gene group sequence of Simultaneously test is lactobacillus paraceasi KL1-Liu bacterial strain application based theoretical.
Probiotic bacterium for human body health-care effect already generally acknowledge by the world, probiotic products has become the first-selection of protective foods.This product had both had abundant nutritive value, had again special physiological function, integrated Nutrition and health care.Probiotics tablets product is one of canonical form of dried product, have directly can chew or swallow, edible easy to carry, stripping and the feature such as absorption rate is very fast.
In recent years along with the development of modern science and technology level and foods processing technique, some reports about probiotic bacterium series products are in succession there are both at home and abroad, " a kind of preparation method of probiotics tablets " of such as Shaanxi Tech Univ's application, number of patent application is: CN101843337 A; " a kind of probiotic bacterium goat milk sheet and preparation method thereof " of Shaanxi Research Institute of Agricultural Products Processing Technology's application, number of patent application is: CN102742659 A; " composite active probiotics milk powder candy " of Wang Shiyi application, number of patent application is: CN102450331A.The original viable count of the Milk Slice of Probiotic AB of people's developments such as Liu Xiuhe can reach 10 9cFU/g; The initial viable count of probiotic milk tablets of Zhou Haijun and Fan Zhao Wuhan Research Institute all only has 10 6about CFU/g.Although above-mentioned patent and document having the report about utilizing probiotic bacterium to prepare tablet form food, there is the method that the lactobacillus paraceasi KL1-Liu reducing serum cholesterol effect prepares probiotics tablets there is not yet domestic and international pertinent literature report and patent report about the product bile salt hydrolase utilizing separation screening in Kefir grain.
Summary of the invention
The object of this invention is to provide a kind of utilization produce bile salt hydrolase and there is the method that the lactobacillus paraceasi KL1-Liu reducing serum cholesterol effect prepares probiotics tablets.
The present invention is achieved by the following technical solutions:
Probiotics tablets formula composition (W/W, massfraction) of the present invention: [viable count is (1.0 ~ 5.0) × 10 to lactobacillus paraceasi KL1-Liu probiotics bacterial powder 0.1% ~ 1.0% 11cFU/g], skim-milk 15% ~ 20%, oligofructose 10% ~ 15%, Xylitol 4% ~ 6%, sucrose 4% ~ 6%, citric acid 0.2% ~ 5%, Magnesium Stearate 0.5% ~ 3.5%, the ratio of Microcrystalline Cellulose and maltodextrin is 1: 5.
In described method, lactobacillus paraceasi (Lactobacillus paracasei) KL1-Liu separation screening from Jilin, the Kefir grain (also known as Kefir granule) of Huhehaote City's average family, and being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 25th, 2012, its preserving number is: CGMCC No.7029 (bacterial strain KL1-Liu).On the MRS selective medium containing calcium carbonate and tennecetin, the bacterium colony size 2 ~ 3mm of lactobacillus paraceasi (Lactobacillus paracasei) KL1-Liu bacterial strain, bacterium colony has comparatively high viscosity, surface irregularity is as flakes, edge is irregular, matt, flat, canescence, translucent; Individual morphology is shaft-like, varying length, short catenation, G +sporeless bacterium.
In described method, lactobacillus paraceasi KL1-Liu probiotics bacterial powder is the mixture of lactobacillus paraceasi KL1-Liu viable bacteria body and compound lyophilized vaccine.
In described method, the preparation process of lactobacillus paraceasi KL1-Liu probiotics bacterial powder is as follows:
(1) adopt vertical and high-speed refrigerated centrifuge by the lactobacillus paraceasi KL1-Liu fermented liquid through enlarged culturing and high density fermentation under 4 DEG C of conditions, the centrifugal 10 ~ 20min of 6000r/min, abandons supernatant liquor, collects and obtains bacterium mud.
(2) in sterilizing freeze-drying bottle, by the amount of centrifugal primary fermentation liquid 1/10 volume, add 12.5% ~ 19.5% maltodextrin+10.5% ~ 3.5% skim-milk, after mixing with the bacterium mud collected, obtain starter culture concentrates.
In aforesaid method, lyophilized vaccine combination formula is (W/V, mass volume ratio concentration): 12.5% ~ 19.5% maltodextrin+10.5% ~ 3.5% skim-milk (Erie's board high protein high calcium skim-milk).
(3) by starter culture concentrates in-40 DEG C of pre-freeze 10h to fully charge state, obtain pre-freeze starter; Utilize U.S. 6L LABCONCO vacuum freeze drier by pre-freeze starter in-55 DEG C, under the condition of vacuum tightness 0.14mBar, freeze-drying 48h, to complete drying state, obtains lactobacillus paraceasi KL1-Liu probiotics bacterial powder.
In described method, the preparation process of lactobacillus paraceasi KL1-Liu probiotics tablets is as follows:
(1) adopt medicinal herb grinder respectively the auxiliary materials such as Xylitol, sucrose, citric acid to be carried out fragmentation, cross 100 mesh sieve for subsequent use.
In aforesaid method, 100 mesh sieve are provided by great Ding screen cloth company limited; Medicinal herb grinder is provided by south of the River Ou Chuan electrical machinery plant, and model is Hc-280.
(2) each composition in adopting 3 D multi-directional movement mixer to be filled a prescription by probiotics tablets, be 10 ~ 20r/min at rotating speed, power of motor is mix under 0.5KW condition, makes probiotics tablets meal.
In aforesaid method, 3 D multi-directional movement mixer is provided by Shanghai Tian Fan pharmaceutical machine factory, and model is HD20.
(3) adopt single-punching tablet press to be 18KN by probiotics tablets meal at peak pressure, pellet diameter is 15 ~ 20mm, under heavy 1g, the hardness 4 ~ 8N condition of every sheet, is pressed into and produces bile salt hydrolase lactobacillus paraceasi KL1-Liu probiotics tablets.
In aforesaid method, single-punching tablet press is provided by Shanghai Tian Fan pharmaceutical machine factory, and model is DP50.
In described method, the main bacteria seed in probiotics tablets is Kefir grain source lactobacillus paraceasi KL1-Liu probiotic bacterium.This bacterium powder has good reduction serum cholesterol effect, and have whole intestines effect, can set up a microecological balance system being conducive to having good health and a long life in enteron aisle, promoting digestion absorbs, and be improved constipation effect, the illnesss such as the cardiovascular disorder of human consumer, gastrointestinal tract disease can be prevented.
Obtained abovely a kind ofly produce bile salt hydrolase and there is lactobacillus paraceasi KL1-Liu method preparing probiotics tablets reducing serum cholesterol effect and products thereof, and special preparing strain belongs to scope.
Accompanying drawing explanation
Produce bile salt hydrolase lactobacillus paraceasi probiotics tablets preparation technology schema to be described as follows:
(1) lactobacillus paraceasi KL1-Liu bacterial strain probiotic bacterium: separation screening from Jilin, the Kefir grain of Huhehaote City's average family.(2) bacterial strain activation, enlarged culturing and high density fermentation: by the lactobacillus paraceasi KL1-Liu bacterial strain that activated through modified MRS culture medium enlarged culturing and high density fermentation, obtain the fermented liquid of higher viable count.(3) centrifugal concentrating and interpolation protective material: by KL1-Liu bacterial strain fermentation liquor under 4 DEG C of conditions, the centrifugal 10 ~ 20min of 6000r/min, abandons supernatant liquor, collects and obtains bacterium mud; In sterilizing freeze-drying bottle, by the amount of centrifugal primary fermentation liquid 1/10 volume, add 12.5% ~ 19.5% maltodextrin+10.5% ~ 3.5% skim-milk, after mixing with the bacterium mud collected, obtain starter culture concentrates.(4) pre-freeze and vacuum lyophilization: by starter culture concentrates in-40 DEG C of pre-freeze 10h to fully charge state, obtain pre-freeze starter; Utilize U.S. 6L LABCONCO vacuum freeze drier by pre-freeze starter in-55 DEG C, under the condition of vacuum tightness 0.14mBar, freeze-drying 48h, to complete drying state, obtains lactobacillus paraceasi KL1-Liu probiotics bacterial powder.(5) auxiliary material is added: skim-milk 15% ~ 20%, oligofructose 10% ~ 15%, Xylitol 4% ~ 6%, sucrose 4% ~ 6%, citric acid 0.2% ~ 5%, Magnesium Stearate 0.5% ~ 3.5%, the ratio of Microcrystalline Cellulose and maltodextrin is 1: 5.(6) auxiliary material broken with sieve: adopt medicinal herb grinder particle to be greater than 100 object auxiliary materials such as the auxiliary materials such as sucrose, citric acid, Xylitol and carry out fragmentation, and adopt 100 object sieve.(7) preparation of probiotics tablets meal: adopt 3 D multi-directional movement mixer probiotics bacterial powder to be mixed the meal making probiotics tablets with auxiliary material, rotating speed used in combination is 10 ~ 20r/min.(8) preparation of probiotics tablets: compressing tablet adopts the peak pressure of single-punching tablet press to be 18KN, and pellet diameter is 15 ~ 20mm, every sheet heavy 1g, hardness 4 ~ 8N.(9) probiotics tablets packaging and storage: be sub-packed in by probiotics tablets in sealing bag or Plastic Bottle, after press mold sealing or gland seal, in 4 DEG C of refrigerator storages, the product viable bacteria quality guaranteed period is 1 year.
The probiotics tablets raw material sources that the present invention makes are convenient, and technique is simple, and fabrication cycle is short, simple to operate, lower to equipment requirements, and finished product probiotics tablets number of viable, apparently higher than like product, is suitable for suitability for industrialized production.
Embodiment
The invention will be further described and do not limit the scope of the invention for following embodiment.
Experimental technique in following embodiment, if no special instructions, is ordinary method.
Percentage composition in following embodiment, if no special instructions, is mass volume ratio concentration (W/V).
The preparation method of embodiment 1, product bile salt hydrolase lactobacillus paraceasi KL1-Liu probiotics tablets
(1) activation of lactobacillus paraceasi KL1-Liu bacterial strain, enlarged culturing and high density fermentation
By the lactobacillus paraceasi KL1-Liu bacterial strain that activated through modified MRS culture medium enlarged culturing and high density fermentation, obtain viable count for (1.0 ~ 5.0) × 10 10the fermented liquid of CFU/mL.
(2) centrifugal concentrating of lactobacillus paraceasi KL 1-Liu bacterial strain and lyophilize
By fermented liquid under 4 DEG C of conditions, the centrifugal 10 ~ 20min of 6000r/min, abandons supernatant liquor, collects and obtains bacterium mud; In sterilizing freeze-drying bottle, by the amount of centrifugal primary fermentation liquid 1/10 volume, add 12.5% ~ 19.5% maltodextrin+10.5% ~ 3.5% skim-milk, after mixing with the bacterium mud collected, obtain starter culture concentrates; By starter culture concentrates in-40 DEG C of pre-freeze 10h to fully charge state, obtain pre-freeze starter; Utilize U.S. 6LLABCONCO vacuum freeze drier by pre-freeze starter in-55 DEG C, under the condition of vacuum tightness 0.14mBar, freeze-drying 48h, to complete drying state, obtains lactobacillus paraceasi KL1-Liu probiotics bacterial powder.
(3) detection of lactobacillus paraceasi KL1-Liu number of viable in probiotics bacterial powder
Taking 25g probiotics bacterial powder puts into containing 225mL sterile saline, adopts slap type homogenizer with the velocity process 1min of 8 000 ~ 10 000r/min, fully after vibration, makes 10 -1even diluent; Do 10 times with 9mL sterile saline again to increase progressively and be diluted to 10 -11; Get 10 -9~ 10 -11the each 1mL of diluent be placed in sterilized petri dishes, pour into and to dissolve and the modified MRS culture medium being cooled to 46 DEG C is about 15mL, rapidly turn plate gently, substratum and bacterium liquid are fully mixed, and each extent of dilution repeats for 3 times.Modified MRS culture medium is injected in the sterilizing plate being added with 1mL stroke-physiological saline solution simultaneously and make blank.After culture medium solidifying, upset is dull and stereotyped, puts in (36 ± 1) DEG C incubator and cultivates (48 ± 2) h, i.e. count enable after bacterium colony grows.Its plate count result shows, and in every 1g probiotics bacterial powder, the number of viable of lactobacillus paraceasi KL1-Liu is (1.0 ~ 5.0) × 10 11cFU.
(4) auxiliary material broken with sieve
Adopt medicinal herb grinder respectively the auxiliary materials such as Xylitol, sucrose, citric acid to be carried out fragmentation, cross 100 mesh sieve for subsequent use.Object is that probiotics bacterial powder is more easily mixed with auxiliary material, and during compressing tablet, uniform particles is easily shaping.
(5) preparation of probiotics tablets meal
Be 1: 5 by the ratio of Microcrystalline Cellulose and maltodextrin, skim-milk 15% ~ 20%, oligofructose 10% ~ 15%, Xylitol 4% ~ 6%, sucrose 4% ~ 6%, citric acid 0.2% ~ 5%, Magnesium Stearate 0.5% ~ 3.5% auxiliary material such as grade and addition be 0.1% ~ 1.0% lactobacillus paraceasi KL1-Liu probiotics bacterial powder [viable count is (1.0 ~ 5.0) × 10 11cFU/g], employing rotating speed is 10 ~ 20r/min, and power of motor is that the 3 D multi-directional movement mixer of 0.5KW fully mixes, and makes probiotics tablets meal.
(6) preparation of probiotics tablets
Adopt single-punching tablet press that the probiotics tablets meal mixed is pressed into probiotics tablets, the maximum tableting pressure of single-punching tablet press is 18KN, and pellet diameter is 15 ~ 20mm, every sheet heavy 1g, hardness 4 ~ 8N.
(7) sensory evaluation of probiotics tablets
Evaluation group is made up of (namely by the composition of personnel being subject to professional sensory evaluation training) professional evaluator, have 15 people, adopt descriptive detection method respectively to quality, mouthfeel, local flavor, outward appearance, the color and luster of sample, carry out careful description, standards of grading are divided into third gear: good, in, differ from three class, the evaluation criteria of orthogonal test of filling a prescription using the result of sensory evaluation as probiotics tablets.The subjective appreciation standard of probiotics tablets and evaluation index are in table 1.
The subjective appreciation standard of table 1 lactobacillus paraceasi probiotics tablets
According to table 1 subjective appreciation standard as the test index evaluating probiotics tablets organoleptic quality, the more excellent combination formula of lactobacillus paraceasi probiotics tablets is determined by orthogonal test, with the probiotics tablets product that more excellent combination formula makes, its endoplasm structure is fine and closely woven evenly and fractography is tight, mouthfeel children is sliding not to stick to one's teeth, and has the distinctive fragrance of milk, profile is complete, smooth, thin and thick is even, neat in edge, uniform color becomes oyster white, and its subjective appreciation mark is the highest.
(8) detection of lactobacillus paraceasi KL1-Liu number of viable in probiotics tablets
Taking 25g probiotics tablets puts into containing 225mL sterile saline, adopts slap type homogenizer with the velocity process 1min of 8 000 ~ 10 000r/min, fully after vibration, makes 10 -1even diluent.Do 10 times with 9mL sterile saline again to increase progressively and be diluted to 10 -11, get 10 -9~ 10 -11the each 1mL of diluent be placed in sterilized petri dishes, pour into and to dissolve and the modified MRS culture medium being cooled to 46 DEG C is about 15mL, rapidly turn plate gently, substratum and bacterium liquid are fully mixed, and each extent of dilution repeats for 3 times.Modified MRS culture medium is injected in the sterilizing plate being added with 1mL stroke-physiological saline solution simultaneously and make blank.After culture medium solidifying, upset is dull and stereotyped, puts in (36 ± 1) DEG C incubator and cultivates (48 ± 2) h, i.e. count enable after bacterium colony grows.Its plate count result shows, and in every 1g probiotics tablets, the number of viable of lactobacillus paraceasi KL1-Liu is (1.0 ~ 5.0) × 10 9cFU, is shown in Table 2.
(9) in probiotics tablets, the lactobacillus paraceasi KL1-Liu viable bacteria quality guaranteed period tests
Lactobacillus paraceasi KL1-Liu probiotics tablets is placed in 4 DEG C of refrigerator storages, the number of viable adopting the method for plate culture count to detect lactobacillus paraceasi KL1-Liu in probiotics tablets every 2 months, detection method is with the operation steps of above-mentioned (8).Its plate count the results are shown in Table 2.
Lactobacillus paraceasi KL1-Liu viable bacteria quality guaranteed period result in table 2 probiotics tablets
From table 1, in probiotics tablets, the viable count detected result of lactobacillus paraceasi KL1-Liu is 4.82 × 10 9cFU/g, the number of viable meeting probiotic products is greater than 10 6the standard of CFU/g.Place after 1 year under refrigeration 4 DEG C of environment, the viable count recording probiotics tablets under same procedure condition is 4.6 × 10 8cFU/g, the number of viable showing this product is still greater than 10 6the standard of CFU/g, the number of viable of probiotic bacterium is apparently higher than like product.Illustrate and lactobacillus paraceasi KL1-Liu probiotics tablets more than at least 1 year viable bacteria quality guaranteed period under 4 DEG C of refrigerated conditions that the present invention makes show that this probiotics tablets has good storage stability.
The number of viable of the product bile salt hydrolase lactobacillus paraceasi KL1-Liu probiotics tablets adopting above-mentioned fermentation production technology, freeze-dry process and probiotics tablets preparation technology to obtain all can reach 10 9more than CFU/g, the number of viable of 4 DEG C of storages, 1 year lactobacillus paraceasi KL1-Liu reaches 10 8more than CFU/g, is greater than 10 6the standard of CFU/g, still can play the health-care effect reducing serum cholesterol.The probiotics tablets raw material sources that the present invention makes are convenient, and technique is simple, and fabrication cycle is short, simple to operate, lower to equipment requirements, is suitable for suitability for industrialized production.Functional foodstuff such as the exploitation of product bile salt hydrolase lactobacillus paraceasi KL1-Liu bacterial strain probiotics tablets, probiotics yogurt etc. is utilized to have wide market application foreground.
Project 1 belonging to this patent: Department of Science and Technology's national science and technology key special subjects " disease-resistant transgenic sheep rearing new variety " sub-problem " foundation that disease-resistant transgenic sheep expansion traditional font is/disease-resistant transgenic goat-anti disease, production performance and safety evaluation " item number: 2013ZX08008-005
The project beginning and ending time: 2013.01-2013.12
Project leader: Liu Hui
Project 2 belonging to this patent: " 12 " field, rural area Country science and technology plan problem " animal-derived food HACCP System Construction and pathogenic bacterium high throughput testing technology "
Item number: 2012BAD28B02-01
The project beginning and ending time: 2012.01-2015.12
Project leader: Kong Baohua
Project 3 belonging to this patent: Department of Science and Technology's Transformation of Agricultural Sci-Tech Achievements capital items " utilizes Kefir grain source to produce bile salt hydrolase milk-acid bacteria and produces functional yoghourt technical transform "
Item number: 2013GB2A000006
The project beginning and ending time: 2013.09-2015.08
Project leader: Bai Yongqiang.

Claims (2)

1. lactobacillus paraceasi (Lactobacillus paracasei) KL 1-Liu CGMCC No.7029.
2. one kind utilizes the method for producing bile salt hydrolase lactobacillus paraceasi (Lactobacillus paracasei) KL1-Liu CGMCC No.7029 bacterial strain and preparing probiotics tablets, it is characterized in that: (1) probiotics tablets formula forms: comprise lactobacillus paraceasi KL1-Liu probiotics bacterial powder 0.1% ~ 1.0%, skim-milk 15% ~ 20%, oligofructose 10% ~ 15%, Xylitol 4% ~ 6%, sucrose 4% ~ 6%, citric acid 0.2% ~ 5%, Magnesium Stearate 0.5% ~ 3.5%, the ratio of Microcrystalline Cellulose and maltodextrin is 1: 5; (2) preparation of KL1-Liu probiotics bacterial powder: by the lactobacillus paraceasi KL1-Liu fermented liquid through enlarged culturing and high density fermentation under 4 DEG C of conditions, the centrifugal 10 ~ 20min of 6000r/min, abandons supernatant liquor, collects and obtains bacterium mud; In sterilizing freeze-drying bottle, by the amount of centrifugal primary fermentation liquid 1/10 volume, add 12.5% ~ 19.5% maltodextrin+10.5% ~ 3.5% skim-milk, after mixing with the bacterium mud collected, obtain starter culture concentrates; By starter culture concentrates in-40 DEG C of pre-freeze 10h to fully charge state, obtain pre-freeze starter; Utilize U.S. 6L LABCONCO vacuum freeze drier by pre-freeze starter in-55 DEG C, under the condition of vacuum tightness 0.14mBar, freeze-drying 48h, to complete drying state, obtains lactobacillus paraceasi KL1-Liu probiotics bacterial powder; (3) preparation of probiotics tablets: adopt medicinal herb grinder respectively the auxiliary materials such as Xylitol, sucrose, citric acid to be carried out fragmentation, cross 100 mesh sieve for subsequent use; Each composition in adopting 3 D multi-directional movement mixer to be filled a prescription by probiotics tablets, be 10 ~ 20r/min at rotating speed, power of motor is mix under 0.5KW condition, makes probiotics tablets meal; Adopt single-punching tablet press to be 18KN by probiotics tablets meal at peak pressure, pellet diameter is 15 ~ 20mm, under heavy 1g, the hardness 4 ~ 8N condition of every sheet, is pressed into and produces bile salt hydrolase lactobacillus paraceasi KL1-Liu probiotics tablets.
CN201410640001.2A 2014-11-14 2014-11-14 Preparation method of lactobacillus paracasei probiotics tablet of bifidobacteria producing bile salt hydrolase Pending CN104328076A (en)

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CN106635886A (en) * 2016-11-18 2017-05-10 北京农学院 Method for reducing egg cholesterol by utilizing lactobacillus paracasei of bile salt producing hydrolase
CN108300669A (en) * 2018-03-26 2018-07-20 贵州大学 A kind of compoiste fermented microbial inoculum of fermented bean curd accelerating and preparation method thereof
CN109996885A (en) * 2017-06-09 2019-07-09 旭兴产株式会社 Exocellular polysaccharide of lactic acid bacteria and application thereof
CN113322216A (en) * 2021-07-30 2021-08-31 北京量化健康科技有限公司 Lactobacillus paracasei B111H and application thereof in metabolic syndrome
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104357359A (en) * 2014-11-14 2015-02-18 北京北农汇邦生物科技有限公司 Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation
CN104450562A (en) * 2014-11-14 2015-03-25 北京农学院 Preparation method of Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase
CN106635886A (en) * 2016-11-18 2017-05-10 北京农学院 Method for reducing egg cholesterol by utilizing lactobacillus paracasei of bile salt producing hydrolase
US11541082B2 (en) 2017-06-05 2023-01-03 Probi Ab Microbial compositions
CN109996885A (en) * 2017-06-09 2019-07-09 旭兴产株式会社 Exocellular polysaccharide of lactic acid bacteria and application thereof
CN108300669A (en) * 2018-03-26 2018-07-20 贵州大学 A kind of compoiste fermented microbial inoculum of fermented bean curd accelerating and preparation method thereof
CN113322216A (en) * 2021-07-30 2021-08-31 北京量化健康科技有限公司 Lactobacillus paracasei B111H and application thereof in metabolic syndrome

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