CN106215168A - Compositions of prevention Age related macular and preparation method thereof - Google Patents
Compositions of prevention Age related macular and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of compositions preventing Age related macular and preparation method thereof, described compositions is formed by the Raw material processing of following weight portion: 10 20 parts of Concha Ostreae polypeptide powder, Radix Ginseng extract 10 20 parts, wolfberry fruit extract 5 10 parts, the preparation method of Concha Ostreae polypeptide powder comprises the steps: (1) Intestinum Stichopi japonici pretreatment;(2) homogenizing of Intestinum Stichopi japonici;(3) primary enzymolysis;(4) secondary enzymolysis;(5) ultrafiltration;(6) nanofiltration;(7) concentrate drying, the compositions rich in nutrition content of prevention Age related macular prepared by the present invention, it is easy to absorbing, absorption rate is high, and impurity and content of beary metal are low, are of high nutritive value, and can supplement the multiple nutrient coming from Concha Ostreae.
Description
Technical field
The present invention relates to a kind of compositions preventing Age related macular and preparation method thereof.
Background technology
Concha Ostreae, accounts for 1/3rd of China's marine aquaculture total output, has cheap, abundance, protein
Content advantages of higher.For a long time, people just recognize the value of Concha Ostreae, it has been carried out Devoting Major Efforts To Developing, has occurred in that one
The oyster product of series, such as lyophilizing Carnis ostreae, Concha Ostreae superfine powder, Concha Ostreae taurine, oyster shell active calcium, oyster peptide etc..Along with
The development of industrial production technology, the continuous appearance of new product, the application of Concha Ostreae is more extensive, but the application to Concha Ostreae is best
No more than utilizing its high protein content to produce polypeptide.
The a lot of active substance of human body, all presented in peptide, does not has peptide, does not just have activity, does not just have life.Peptide
Relating to the hormone of human body, nerve, cell growth and each field of reproduction, its importance is to regulate each system internal and cell
Physiological function, activates internal relevant enzyme system, promotes the permeability of intermediate supersession film, or transcribes by controlling DNA or affect special
Albumen synthesis, final produce specific physiological effect.The present invention provides compositions and the preparation side thereof of prevention Age related macular
Method, old man's immunity of organisms in improvement, the generation of prevention Age related macular.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, it is provided that a kind of compositions preventing Age related macular and
Preparation method, the compositions rich in nutrition content of prevention Age related macular prepared by the method, it is easy to absorb, impurity and weight
Tenor is low, old man's immunity of organisms in improvement, the generation of prevention Age related macular.
The technical solution adopted for the present invention to solve the technical problems is:
The compositions of prevention Age related macular, described compositions is formed by the Raw material processing of following weight portion: Concha Ostreae polypeptide
Powder 10-20 part, Radix Ginseng extract 10-20 part, wolfberry fruit extract 5-10 part, the preparation method of Concha Ostreae polypeptide powder includes walking as follows
Rapid:
(1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 6-8%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Concha Ostreae, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby;
Rich in silt, heavy metal, salinity in Concha Ostreae, want to obtain the Concha Ostreae polypeptide powder of high-quality, need fully to remove
Stating impurity, the design concept of the present invention is as follows: (1) water-insoluble glucan suspension is the aqueous suspension of macromole, macromole
Glucosan itself has the effect of flocculation of reuniting, and can fully adsorb or the impurity such as the mud that flocculates in Concha Ostreae and beavy metal impurity,
The especially removal effect of heavy metal is splendid, and after using glucosan suspension pretreating agent pretreatment, the extensibility of Concha Ostreae is all
Excellent, follow-up homogenizing, the effect of enzymolysis are substantially improved, it addition, glucosan safety non-toxic, the glucosan of residual has increase to exempt from the contrary
Epidemic disease power, the effect of immunologic function improved, it is provided that the nutritive value of Concha Ostreae polypeptide powder;(2) reduced glutathion aqueous solution has
Good activating cell and the function of tissue, can improve the active oxygen in cell or tissue and osmotic pressure, maintains oyster cell
With the fresh and healthy state of tissue long period, it is substantially improved the effect of follow-up ferment treatment, glutathion also safety non-toxic, residual
Glutathion have the function improving immune system, removing toxic substances on the contrary, improve the nutritive value of Concha Ostreae polypeptide powder, improve the product of finished product
Matter, reduces Heavy Metal Pollution risk that may be present in Concha Ostreae polypeptide powder further or eliminates;It addition, the reduced form paddy of residual
The sweet peptide of Guang plays the effect of antioxidant, and composition quality is stable, long shelf-life, edible safety;
(2) homogenizing of Concha Ostreae: step (1) pretreated Concha Ostreae is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Concha Ostreae homogenizing fluid;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Appropriate food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Concha Ostreae, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer, remove the fat in Concha Ostreae, Ke Yigai by lipase enzymolysis
The quality of kind Concha Ostreae polypeptide powder, extends the shelf-life of Concha Ostreae polypeptide powder;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Concha Ostreae secondary enzymolysis supernatant, pass through
Protein in Concha Ostreae is fully degraded into aminoacid and micromolecule polypeptide by enzymolysis, it is simple to the absorption of Concha Ostreae polypeptide powder and utilization,
Utilization rate is high, is of high nutritive value;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 5000-8000Da must be surpassed
Filter permeate;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is for 150-300Da, by nanofiltration to Concha Ostreae polypeptide powder desalting processing, it is thus achieved that Concha Ostreae polypeptide powder salt divide low, in good taste;
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most chilled
It is dried to obtain powder food product level Concha Ostreae polypeptide.
Preferably, described compositions is formed by the Raw material processing of following weight portion: 15 parts of Concha Ostreae polypeptide powder, Radix Ginseng extract
15 parts, wolfberry fruit extract 6 parts.
Preferably, in step (1), pretreating agent A is the water-insoluble glucan suspension of mass fraction 7%, described pre-place
Reason agent B is the reduced glutathion aqueous solution of mass fraction 1.0%.
Preferably, temperature 35-65 DEG C of step (2) mesohigh homogenizing, pressure 120-400Mpa, circulates homogenizing at least two
Secondary.
Preferably, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is the 0.2-of fresh Concha Ostreae quality
0.8%.
Preferably, in described step (4), the enzyme activity of compound protease and neutral protease is than for 3:1, and enzyme is always used
Amount is the 0.6-1.0% of fresh Concha Ostreae quality.
A kind of preparation method of the compositions preventing Age related macular, described preparation method is carried out as follows:
The first step: prepare Concha Ostreae polypeptide powder,
(1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Concha Ostreae, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby;
(2) homogenizing of Concha Ostreae: step (1) pretreated Concha Ostreae is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Concha Ostreae homogenizing fluid;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Appropriate food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Concha Ostreae, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Concha Ostreae secondary enzymolysis supernatant;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 5000-8000Da must be surpassed
Filter permeate;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is 150-300Da;
Second step, mixes in proportion Concha Ostreae polypeptide powder, Radix Ginseng extract and the wolfberry fruit extract of formula ratio, then
Sterilized, fill becomes capsule.
The invention has the beneficial effects as follows: the compositions rich in nutrition content of prevention Age related macular prepared by the present invention, easily
In absorbing, absorption rate is high, and impurity and content of beary metal are low, are of high nutritive value, and can supplement and multiple come from Concha Ostreae
Nutrient, improve immunity of organisms, prevention Age related macular generation.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail.
Embodiment 1:
The compositions of prevention Age related macular, described compositions is formed by the Raw material processing of following weight portion: Concha Ostreae polypeptide
10 parts of powder, Radix Ginseng extract 10 parts, wolfberry fruit extract 5 parts, the preparation method of compositions comprises the steps:
The first step, prepares Concha Ostreae polypeptide powder, (1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to enter
Row pretreatment, pretreating agent A is the water-insoluble glucan suspension of mass fraction 8%, and described pretreating agent B is mass fraction
The reduced glutathion aqueous solution of 0.5%, concrete pretreatment operation is: first use pretreating agent A pretreatment 2h under room temperature,
Solid-liquid ratio is 1:2, then stirs pretreatment 2h under room temperature with pretreating agent B, then stands 45minh, and solid-liquid ratio is 1:1;
(2) homogenizing of Concha Ostreae: step (1) pretreated Concha Ostreae is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Concha Ostreae homogenizing fluid, high pressure homogenize temperature 35-45 DEG C, pressure 120Mpa, circulation homogenizing 3 twice;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 35 DEG C, adjusts pH value to 6.5, adds appropriate food
Grade lipase, stirs, enzymolysis 1h, and Concha Ostreae is carried out defat, then heats to 75 DEG C of inactivation 3min, is often subsequently cooled to
The gentle and quiet 1h that puts, removes supernatant oil layer, and the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Concha Ostreae quality
0.2%;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40 DEG C, adjusts pH value 6.5, adds appropriate
Complex food level protease hydrolyzed 2h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 10min, be then centrifuged for separating, obtain Concha Ostreae secondary enzymolysis supernatant, compound protease
With the enzyme activity of neutral protease than for 3:1, the total consumption of enzyme is the 0.6% of fresh Concha Ostreae quality;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 5000-2000Da must be surpassed
Filter permeate;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-200Da;
Second step,
Concha Ostreae polypeptide powder, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, the most sterilized,
Fill prepares capsule.
Embodiment 2
The compositions of prevention Age related macular, described compositions is formed by the Raw material processing of following weight portion: Concha Ostreae polypeptide
20 parts of powder, Radix Ginseng extract 20 parts, wolfberry fruit extract 10 parts, the preparation method of compositions comprises the steps:
Described compositions is prepared as follows:
The first step, prepares Concha Ostreae polypeptide powder, (1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to enter
Row pretreatment, pretreating agent A is the water-insoluble glucan suspension of mass fraction 6%, and described pretreating agent B is mass fraction
The reduced glutathion aqueous solution of 1.5%, concrete pretreatment operation is: first use pretreating agent A pretreatment 1h under room temperature,
Solid-liquid ratio is 1:3, then stirs pretreatment 3h under room temperature with pretreating agent B, then stands 45min, and solid-liquid ratio is 1:1;
(2) homogenizing of Concha Ostreae: step (1) pretreated Concha Ostreae is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Concha Ostreae homogenizing fluid, temperature 60-65 DEG C of high pressure homogenize, pressure 400Mpa, circulation homogenizing twice;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 45 DEG C, adjusts pH value to 7.5, adds appropriate food
Grade lipase, stirs, enzymolysis 1h, and Concha Ostreae is carried out defat, then heats to 75 DEG C of inactivation 5min, is often subsequently cooled to
The gentle and quiet 2h that puts, removes supernatant oil layer;The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Concha Ostreae quality
0.8%;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Concha Ostreae secondary enzymolysis supernatant;Compound
The enzyme activity of flavor protease and neutral protease is than for 3:1, and the total consumption of enzyme is the 1.0% of fresh Concha Ostreae quality;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 6000-8000Da must be surpassed
Filter permeate;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
200-300Da;
Second step,
Concha Ostreae polypeptide powder, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, the most sterilized,
Fill prepares capsule.
Embodiment 3:
The compositions of prevention Age related macular, described compositions is formed by the Raw material processing of following weight portion: Concha Ostreae polypeptide
15 parts of powder, Radix Ginseng extract 15 parts, wolfberry fruit extract 6 parts, the preparation method of peptide composition comprises the steps:
Described compositions is prepared as follows:
The first step, prepares Concha Ostreae polypeptide powder, (1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to enter
Row pretreatment, pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, and described pretreating agent B is that quality is divided
The reduced glutathion aqueous solution of several 1.0%, concrete pretreatment operation is: by pretreating agent A and pretreating agent B with volume ratio
1:1 mix homogeneously, is subsequently adding fresh Concha Ostreae, stir process 2.5h under room temperature, then stands 1h, solid-liquid ratio 1:2, locates in advance
After reason, solid-liquid separation is standby;
(2) homogenizing of Concha Ostreae: step (1) pretreated Concha Ostreae is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Concha Ostreae homogenizing fluid, temperature 40-45 DEG C of high pressure homogenize, pressure 180Mpa, circulation homogenizing three times;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 40 DEG C, adjusts pH value to 7.0, adds appropriate food
Grade lipase, stirs, enzymolysis 1h, and Concha Ostreae is carried out defat, then heats to 75 DEG C of inactivation 5min, is often subsequently cooled to
The gentle and quiet 2h that puts, removes supernatant oil layer;The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Concha Ostreae quality
0.5%;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 45 DEG C, adjusts pH value 6.8, adds appropriate
Complex food level protease hydrolyzed 2.5h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 12min, be then centrifuged for separating, obtain Concha Ostreae secondary enzymolysis supernatant;Compound protease
With the enzyme activity of neutral protease than for 3:1, the total consumption of enzyme is the 0.75% of fresh Concha Ostreae quality;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 6000Da is obtained ultrafiltration saturating
Cross liquid;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.3 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
220Da;
Second step,
Concha Ostreae polypeptide powder, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, the most sterilized,
Fill prepares capsule.
The compositions of prevention Age related macular prepared by the present invention includes the multiple element that Concha Ostreae self contains, nutritional labeling
Abundant, rich in several amino acids, small-molecular peptides, it is of high nutritive value, it is easy to absorb, absorption rate is high, and impurity content is low,
Heavy metal does not detects, and prevention Age related macular effect is notable.
Embodiment described above is the one preferably scheme of the present invention, not makees the present invention any pro forma
Limit, on the premise of without departing from the technical scheme described in claim, also have other variant and remodeling.
Claims (7)
1. prevent the compositions of Age related macular, it is characterised in that: described compositions is formed by the Raw material processing of following weight portion:
Concha Ostreae polypeptide powder 10-20 part, Radix Ginseng extract 10-20 part, wolfberry fruit extract 5-10 part, the preparation method of Concha Ostreae polypeptide powder includes
Following steps:
(1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is that quality is divided
The water-insoluble glucan suspension of number 6-8%, described pretreating agent B is the reduced form gluathione of mass fraction 0.5-1.5%
Peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-3, then
Stirring pretreatment 2-3h under room temperature with pretreating agent B, then stand 45min-1h, solid-liquid ratio is 1:1-2, or by pretreatment
Agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Concha Ostreae, stir process 2-2.5h under room temperature,
Then standing 0.5-1h, solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby;
(2) homogenizing of Concha Ostreae: be placed in high pressure homogenizer by step (1) pretreated Concha Ostreae, add appropriate water, is carried out all
Matter, obtains Concha Ostreae homogenizing fluid;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 0.5-1h, and Concha Ostreae is carried out defat, then heats to 75 DEG C of inactivation 3-5min, then
It is cooled to room temperature and stands 1-2h, remove supernatant oil layer;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Concha Ostreae secondary enzymolysis supernatant;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration saturating
Cross liquid;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da;
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder food product level Concha Ostreae polypeptide.
Prevent the compositions of Age related macular the most according to claim 1, it is characterised in that: described compositions is by following weight
The Raw material processing of part forms: 15 parts of Concha Ostreae polypeptide powder, Radix Ginseng extract 15 parts, wolfberry fruit extract 6 parts.
The compositions of prevention Age related macular the most according to claim 1, it is characterised in that: pretreating agent A in step (1)
For the water-insoluble glucan suspension of mass fraction 7%, described pretreating agent B is the reduced form paddy Guang of mass fraction 1.0%
Sweet peptide aqueous solution.
The compositions of prevention Age related macular the most according to claim 1, it is characterised in that: step (2) mesohigh homogenizing
Temperature 35-65 DEG C, pressure 120-400Mpa, circulation homogenizing at least twice.
The compositions of prevention Age related macular the most according to claim 1, it is characterised in that: described food-grade lipase
Enzyme work is 20,000 U/g, and enzyme dosage is the 0.4-0.8% of fresh Concha Ostreae quality.
Compositions of prevention Age related macular the most according to claim 1 and preparation method thereof, it is characterised in that: described step
Suddenly in (4), the enzyme activity of compound protease and neutral protease is than for 3:1, and the total consumption of enzyme is the 0.6-of fresh Concha Ostreae quality
1.0%.
7. described in a claim 1-6 any one, prevent the preparation method of the compositions of Age related macular, it is characterised in that
Described preparation method is carried out as follows:
The first step: prepare Concha Ostreae polypeptide powder;
(1) Concha Ostreae pretreatment: collect fresh Concha Ostreae, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is that quality is divided
The water-insoluble glucan suspension of number 6-8%, described pretreating agent B is the reduced form gluathione of mass fraction 0.5-1.5%
Peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-3, then
Stirring pretreatment 2-3h under room temperature with pretreating agent B, then stand 45min-1h, solid-liquid ratio is 1:1-2, or by pretreatment
Agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Concha Ostreae, stir process 2-2.5h under room temperature,
Then standing 0.5-1h, solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby;
(2) homogenizing of Concha Ostreae: be placed in high pressure homogenizer by step (1) pretreated Concha Ostreae, add appropriate water, is carried out all
Matter, obtains Concha Ostreae homogenizing fluid;
(3) primary enzymolysis: step (2) gained Concha Ostreae homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 0.5-1h, and Concha Ostreae is carried out defat, then heats to 75 DEG C of inactivation 3-5min, then
It is cooled to room temperature and stands 1-2h, remove supernatant oil layer;
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Concha Ostreae secondary enzymolysis supernatant;
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Concha Ostreae secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration saturating
Cross liquid;
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da;
Second step, mixes in proportion Concha Ostreae polypeptide powder, Radix Ginseng extract and the wolfberry fruit extract of formula ratio, then through going out
Bacterium, fill become capsule.
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