CN106309306A - Skin care facial cleanser and preparation method thereof - Google Patents
Skin care facial cleanser and preparation method thereof Download PDFInfo
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- CN106309306A CN106309306A CN201610682824.0A CN201610682824A CN106309306A CN 106309306 A CN106309306 A CN 106309306A CN 201610682824 A CN201610682824 A CN 201610682824A CN 106309306 A CN106309306 A CN 106309306A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/00—Preparations for care of the skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention relates to skin care facial cleanser and a preparation method thereof. The skin care facial cleanser is prepared from the following raw materials: 30-40 parts of sea cucumber intestine extract, 10-18 parts of aloe juice, 1-5 parts of reduced glutathione, 2-6 parts of nano-bamboo charcoal powder, 2-8 parts of hyaluronic acid, 20-30 parts of honey and 50-80 parts of deionized water, wherein the sea cucumber intestine extract is prepared by the following steps: (1) sea cucumber intestine pretreatment; (2) sea cucumber intestine homogenization; (3) primary enzymolysis; (4) secondary enzymolysis; (5) ultrafiltration; (6) nanofiltration; (7) concentration and drying. The prepared skin care facial cleanser takes the sea cucumber intestine extract, the aloe juice, the reduced glutathione, the nano-bamboo charcoal powder, the hyaluronic acid and the honey as effective ingredients, the deionized water is taken as the solvent, all the raw materials are mixed to form the skin care facial cleanser which is rich in nutritional ingredient and low in heavy metal content, and the functions of protecting skin, delaying senescence, whitening and removing freckles are effectively realized by the aid of the synergistic effect among all components.
Description
Technical field
The present invention relates to a kind of skin protection facial milk cleanser and preparation method thereof.
Background technology
Stichopus japonicus has been proved to rich in natural collagen protein, polypeptide, sphingosine, Saponin, mucopolysaccharide, the life of multiple dimension
The mineral such as element, the aminoacid of 18 kinds of human body needs and ferrum, phosphorus, manganese, selenium, iodine, calcium, magnesium, zinc, 50 multiple nutrients become altogether
Point, nutritional labeling contained in the leftover bits and pieces-Intestinum Stichopi japonici during Holothurian machining, no less than body wall, the profit of Intestinum Stichopi japonici resource
With day by day causing attention, after Intestinum Stichopi japonici is manually removed sand by a lot of enterprises, clean, cold air drying or lyophilization, then beat
Mill or micronizing, make capsule product.But Intestinum Stichopi japonici is not carried out a biological disposal upon by this kind of capsule product, and absorption rate is low,
Nutritive value is had a greatly reduced quality.It addition, there is also in the process of raw material: Intestinum Stichopi japonici manually go that husky mud efficiency is low and silt is the most thorough
The end, not thorough with water cleaning, desalting, cause the problem that in product, content of beary metal and salinity exceed standard.
It addition, composition contained in above-mentioned Stichopus japonicus is such as: the Multiple components such as collagen protein, ceramide, Saponin, aminoacid are all
It is the perfect natural materials that skin care is had effect conscientiously, therefore, using the extracting solution containing Stichopus japonicus composition as skin protection
Cosmetic is directly applied on skin, can more directly, more effectively play protection skin, the effect of slow down aging.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, it is provided that a kind of skin protection facial milk cleanser and preparation method thereof, should
Skin protection facial milk cleanser rich in nutrition content prepared by method, it is easy to absorb, directly, more effectively plays protection skin, delays
Old and feeble effect.
The technical solution adopted for the present invention to solve the technical problems is:
Skin protection facial milk cleanser, described skin protection facial milk cleanser is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract 30-40
Part, Aloe juice 10-18 part, reduced glutathion 1-5 part, nano bamboo powdered carbon 2-6 part, hyaluronic acid 2-8 part, Mel 20-30
Part, deionized water 50-80 part, the preparation method of Intestinum Stichopi japonici extract comprises the steps: (1) Intestinum Stichopi japonici pretreatment: collect new
Fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is that the water-insoluble Portugal of mass fraction 8-12% gathers
Sugar suspension, described pretreating agent B is the reduced glutathion aqueous solution of mass fraction 0.5-1.5%, and concrete pretreatment is grasped
As: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-3, then stirs under room temperature with pretreating agent B
Mixing pretreatment 2-3h, then stand 45min-1h, solid-liquid ratio is 1:1-2, or by pretreating agent A and pretreating agent B with volume
Ratio 1:1 mix homogeneously, is subsequently adding fresh Intestinum Stichopi japonici, and then stir process 2-2.5h under room temperature stands 0.5-1h, feed liquid
Ratio 1:2, after pretreatment, solid-liquid separation is standby；
Rich in silt, heavy metal, salinity in Intestinum Stichopi japonici, want to obtain the Intestinum Stichopi japonici extract of high-quality, need fully to go
Except above-mentioned impurity, the design concept of the present invention is as follows: (1) water-insoluble glucan suspension is the aqueous suspension of macromole, greatly
Molecule glucosan itself has the effect of flocculation of reuniting, and can fully adsorb or the mud that flocculates in Intestinum Stichopi japonici and beavy metal impurity etc.
The removal effect of impurity, especially heavy metal is splendid, after using glucosan suspension pretreating agent pretreatment, and stretching of Intestinum Stichopi japonici
Malleability is the most excellent, and follow-up homogenizing, the effect of enzymolysis are substantially improved, it addition, glucosan safety non-toxic, the glucosan of residual has on the contrary
Increase immunity, improve effect of immunologic function；(2) reduced glutathion aqueous solution has good activating cell and tissue
Function, can improve the active oxygen in cell or tissue and osmotic pressure, maintain the new of Intestinum Stichopi japonici cell and tissue long period
Fresh health status, is substantially improved the effect of follow-up ferment treatment, glutathion also safety non-toxic, and the glutathion of residual has on the contrary and changes
Kind immune system, whitening, the function of removing toxic substances, reduce further by Heavy Metal Pollution risk that may be present in Intestinum Stichopi japonici extract
Or eliminate；It addition, the reduced glutathion of residual plays the effect of antioxidant, skin protection facial milk cleanser steady quality, shelf-life
Long, use safety；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer, remove the fat in Intestinum Stichopi japonici by lipase enzymolysis
Fat, can improve the quality of Intestinum Stichopi japonici extract, extends the shelf-life of Intestinum Stichopi japonici extract；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant, logical
Cross enzymolysis and the protein in Intestinum Stichopi japonici be fully degraded into aminoacid and micromolecule polypeptide, it is simple to the absorption of Intestinum Stichopi japonici extract with
Utilizing, utilization rate is high, is of high nutritive value；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is for 150-300Da, by nanofiltration to Intestinum Stichopi japonici extract desalting processing, it is thus achieved that Intestinum Stichopi japonici extract salinity low, in good taste；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most chilled
It is dried to obtain powder Intestinum Stichopi japonici extract.
Preferably, described skin protection facial milk cleanser is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract 35 parts, Aloe
15 parts of juice, reduced glutathion 3 parts, nano bamboo powdered carbon 4 parts, hyaluronic acid 5 parts, Mel 25 parts, deionized water 65 parts.
Preferably, in step (1), pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, described pre-
Inorganic agent B is the reduced glutathion aqueous solution of mass fraction 1.0%.
Preferably, temperature 35-65 DEG C of step (2) mesohigh homogenizing, pressure 120-400Mpa, circulates homogenizing at least two
Secondary.
Preferably, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is the 0.2-of fresh Intestinum Stichopi japonici quality
0.8%.
Preferably, in described step (4), the enzyme activity of compound protease and neutral protease is than for 2-3:1, and enzyme is total
Consumption is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
A kind of preparation method of skin protection facial milk cleanser, described preparation method is carried out as follows:
The first step: prepare Intestinum Stichopi japonici extract,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, pretreating agent A
For the water-insoluble glucan suspension of mass fraction 8-12%, described pretreating agent B is the reduction of mass fraction 0.5-1.5%
Type glutathion aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is
1:2-3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or
Pretreating agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, under room temperature at stirring
Reason 2-2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is 150-300Da；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most chilled
It is dried to obtain powder Intestinum Stichopi japonici extract；
Second step, prepares skin protection facial milk cleanser finished product,
By the Intestinum Stichopi japonici extract of formula ratio, Aloe juice, glutathion, nano bamboo powdered carbon, hyaluronic acid, Mel and go from
Sub-water mix homogeneously, filters off impurity, is filled into bottle.
The invention has the beneficial effects as follows: skin protection facial milk cleanser prepared by the present invention is by Intestinum Stichopi japonici extract, Aloe juice, reduced form
Glutathion, nano bamboo powdered carbon, hyaluronic acid and Mel are as effective ingredient, and deionized water is as solvent, and wherein Intestinum Stichopi japonici carries
Take thing rich in the multiple perfect natural materials that skin care is had effect conscientiously such as ceramide, Saponin, aminoacid, Aloe
Cell, rich in several amino acids and natural whiting Moisture factor, rich in multiple monosaccharide and polysaccharide, vitamin and mineral, is declined by juice
Always improving significantly, the aloe-emodin additionally contained can promote wound healing, and reduced glutathion has whitening and dispels
Effect of speckle, nano bamboo powdered carbon can with the impurity in active adsorption pore, hyaluronic acid have lock water moisture-keeping functions, Mel rich in
Above-mentioned various raw materials are mixed by several amino acids, vitamin, constitute rich in nutrition content, it is easy to absorb, and absorb
The skin protection facial milk cleanser that utilization rate height, impurity and content of beary metal are low, synergism between each component, directly, more effectively plays
Protection skin, slow down aging, whitening and speckle dispelling and the effect of moisturizing.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail.
Embodiment 1:
Skin protection facial milk cleanser, described skin protection facial milk cleanser is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract 30 parts,
Aloe juice 10 parts, reduced glutathion 1 part, nano bamboo powdered carbon 2 parts, hyaluronic acid 2 parts, Mel 20 parts, deionized water 50 parts,
The preparation method of Intestinum Stichopi japonici extract comprises the steps:
Described skin protection facial milk cleanser is prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent
A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 8%, and described pretreating agent B is matter
The reduced glutathion aqueous solution of amount mark 0.5%, concrete pretreatment operation is: first use pretreating agent A pre-under room temperature
Processing 2h, solid-liquid ratio is 1:2, then stirs pretreatment 2h under room temperature with pretreating agent B, then stands 45minh, and solid-liquid ratio is
1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, high pressure homogenize temperature 35-45 DEG C, pressure 120Mpa, circulation homogenizing 3 twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35 DEG C, adjusts pH value to 6.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3min, then cools down
Standing 1h to room temperature, remove supernatant oil layer, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is new fresh sea cucumber
The 0.2% of intestinal quality；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40 DEG C, adjusts pH value 6.5, adds appropriate
Complex food level protease hydrolyzed 2h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 10min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant, composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2:1, and the total consumption of enzyme is the 0.6% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-2000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-200Da；
(7) concentrate drying: it is 30% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder Intestinum Stichopi japonici extract；
Second step, prepares skin protection facial milk cleanser finished product,
By the Intestinum Stichopi japonici extract of formula ratio, Aloe juice, glutathion, nano bamboo powdered carbon, hyaluronic acid, Mel and go from
Sub-water mix homogeneously, filters off impurity, is filled into bottle.
Embodiment 2
Skin protection facial milk cleanser, described skin protection facial milk cleanser is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract 40 parts,
Aloe juice 18 parts, reduced glutathion 5 parts, nano bamboo powdered carbon 6 parts, hyaluronic acid 8 parts, Mel 30 parts, deionized water 80 parts,
The preparation method of Intestinum Stichopi japonici extract comprises the steps:
Described skin protection facial milk cleanser is prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent
A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 12%, and described pretreating agent B is
The reduced glutathion aqueous solution of mass fraction 1.5%, concrete pretreatment operation is: first use pretreating agent A under room temperature
Pretreatment 1h, solid-liquid ratio is 1:3, then stirs pretreatment 3h under room temperature with pretreating agent B, then stands 45min, and solid-liquid ratio is
1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 60-65 DEG C of high pressure homogenize, pressure 400Mpa, circulation homogenizing twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 45 DEG C, adjusts pH value to 7.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.8% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；Multiple
Closing the enzyme activity of flavor protease and neutral protease ratio for 3:1, the total consumption of enzyme is the 1.0% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
200-300Da；
(7) concentrate drying: it is 40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder Intestinum Stichopi japonici extract；
Second step, prepares skin protection facial milk cleanser finished product,
By the Intestinum Stichopi japonici extract of formula ratio, Aloe juice, glutathion, nano bamboo powdered carbon, hyaluronic acid, Mel and go from
Sub-water mix homogeneously, filters off impurity, is filled into bottle.
Embodiment 3:
Skin protection facial milk cleanser, described skin protection facial milk cleanser is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract 35 parts,
Aloe juice 15 parts, reduced glutathion 3 parts, nano bamboo powdered carbon 4 parts, hyaluronic acid 5 parts, Mel 25 parts, deionized water 65 parts,
The preparation method of Intestinum Stichopi japonici extract comprises the steps:
Described skin protection facial milk cleanser is prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent
A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, and described pretreating agent B is
The reduced glutathion aqueous solution of mass fraction 1.0%, concrete pretreatment operation is: by pretreating agent A and pretreating agent B with
Volume ratio 1:1 mix homogeneously, is subsequently adding fresh Intestinum Stichopi japonici, and then stir process 2.5h under room temperature stands 1h, solid-liquid ratio
1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 40-45 DEG C of high pressure homogenize, pressure 180Mpa, circulation homogenizing three times；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 40 DEG C, adjusts pH value to 7.0, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.5% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 45 DEG C, adjusts pH value 6.8, adds appropriate
Complex food level protease hydrolyzed 2.5h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 12min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant；Composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2.5:1, and the total consumption of enzyme is the 0.75% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.3 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
220Da；
(7) concentrate drying: it is 35% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder Intestinum Stichopi japonici extract；
Second step, prepares skin protection facial milk cleanser finished product,
By the Intestinum Stichopi japonici extract of formula ratio, Aloe juice, glutathion, nano bamboo powdered carbon, hyaluronic acid, Mel and go from
Sub-water mix homogeneously, filters off impurity, is filled into bottle.
The skin protection facial milk cleanser of the present invention, takes appropriate uniform application after face, 10min with warm water cleaning, every day during use
The most once, using 7 days continuously, skin is obviously improved.
Skin protection facial milk cleanser prepared by the present invention is by Intestinum Stichopi japonici extract, Aloe juice, reduced glutathion, bamboo charcoal nano
Powder, hyaluronic acid and Mel as effective ingredient, deionized water as solvent, wherein Intestinum Stichopi japonici extract rich in ceramide,
The multiple perfect natural materials to skin care with effect conscientiously such as Saponin, aminoacid, Aloe juice is rich in several amino acids
With natural whiting Moisture factor, rich in multiple monosaccharide and polysaccharide, vitamin and mineral, cell ageing is improved significantly,
The aloe-emodin additionally contained can promote wound healing, and reduced glutathion has effect of whitening and speckle dispelling, nano bamboo
Powdered carbon can be with the impurity in active adsorption pore, and hyaluronic acid has lock water moisture-keeping functions, and Mel is raw rich in several amino acids, dimension
Above-mentioned various raw materials are mixed by element, constitute rich in nutrition content, it is easy to absorb, and absorption rate is high, impurity and
The skin protection facial milk cleanser that content of beary metal is low, synergism between each component, directly, more effectively plays protection skin, delays to decline
Old, whitening and speckle dispelling and the effect of moisturizing.
Embodiment described above is the one preferably scheme of the present invention, not makees the present invention any pro forma
Limit, on the premise of without departing from the technical scheme described in claim, also have other variant and remodeling.
Claims (7)
1. skin protection facial milk cleanser, it is characterised in that: described skin protection facial milk cleanser is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici carries
Take thing 30-40 part, Aloe juice 10-18 part, reduced glutathion 1-5 part, nano bamboo powdered carbon 2-6 part, hyaluronic acid 2-8 part,
Mel 20-30 part, deionized water 50-80 part, the preparation method of Intestinum Stichopi japonici extract comprises the steps:
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder Intestinum Stichopi japonici extract.
Skin protection facial milk cleanser the most according to claim 1, it is characterised in that: described skin protection facial milk cleanser is by the raw material of following weight portion
Process: Intestinum Stichopi japonici extract 35 parts, Aloe juice 15 parts, reduced glutathion 3 parts, nano bamboo powdered carbon 4 parts, hyaluronic acid
5 parts, Mel 25 parts, deionized water 65 parts.
Skin protection facial milk cleanser the most according to claim 1, it is characterised in that: in step (1), pretreating agent A is mass fraction
The water-insoluble glucan suspension of 10%, described pretreating agent B is that the reduced glutathion of mass fraction 1.0% is water-soluble
Liquid.
Skin protection facial milk cleanser the most according to claim 1, it is characterised in that: temperature 35-65 of step (2) mesohigh homogenizing
DEG C, pressure 120-400Mpa, circulation homogenizing is at least twice.
Skin protection facial milk cleanser the most according to claim 1, it is characterised in that: the enzyme work of described food-grade lipase is 20,000 U/
G, enzyme dosage is the 0.4-0.8% of fresh Intestinum Stichopi japonici quality.
Skin protection facial milk cleanser the most according to claim 1, it is characterised in that: in described step (4) compound protease and
The enzyme activity of neutral protease is than for 2-3:1, and the total consumption of enzyme is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
7. the preparation method of skin protection facial milk cleanser described in a claim 1-6 any one, it is characterised in that described preparation method
Carry out as follows:
The first step: prepare Intestinum Stichopi japonici extract,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder Intestinum Stichopi japonici extract；
Second step, prepares skin protection facial milk cleanser finished product,
By the Intestinum Stichopi japonici extract of formula ratio, Aloe juice, glutathion, nano bamboo powdered carbon, hyaluronic acid, Mel and deionized water
Mix homogeneously, filters off impurity, is filled into bottle.
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| CN108852992A (en) * | 2018-08-10 | 2018-11-23 | 武汉乐神三宝蜂业有限公司 | Crease-resistant face cleaning honey of compound royal jelly and preparation method thereof |
| IT201800004332A1 (en) * | 2018-04-09 | 2019-10-09 | Liquid composition for use in the treatment of the mucous membrane of the oro-pharyngo-laryngeal-esophageal tract | |
| CN110613675A (en) * | 2019-10-28 | 2019-12-27 | 烟台华康海洋食品有限公司 | Formula and preparation process of sea cucumber facial cleanser |
| CN113425618A (en) * | 2020-03-23 | 2021-09-24 | 王福芳 | Composite active peptide facial cleanser and preparation method thereof |
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| CN102836103A (en) * | 2012-09-24 | 2012-12-26 | 浙江建中竹业科技有限公司 | Bamboo charcoal facial cleanser |
| CN103585079A (en) * | 2013-10-31 | 2014-02-19 | 尹秀芳 | Efficient moisture sustaining facial mask |
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| CN1871945A (en) * | 2005-06-01 | 2006-12-06 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
| CN102836103A (en) * | 2012-09-24 | 2012-12-26 | 浙江建中竹业科技有限公司 | Bamboo charcoal facial cleanser |
| CN103585079A (en) * | 2013-10-31 | 2014-02-19 | 尹秀芳 | Efficient moisture sustaining facial mask |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| IT201800004332A1 (en) * | 2018-04-09 | 2019-10-09 | Liquid composition for use in the treatment of the mucous membrane of the oro-pharyngo-laryngeal-esophageal tract | |
| WO2019197986A1 (en) * | 2018-04-09 | 2019-10-17 | Sofar Swiss Sa | Liquid composition for use in the treatment of the mucosa of the oro-pharyngo-laryngo-esophageal tract |
| CN112055594A (en) * | 2018-04-09 | 2020-12-08 | 索法尔瑞士有限公司 | Liquid composition for treating mucosa of oropharyngeal laryngeal tract |
| CN108852992A (en) * | 2018-08-10 | 2018-11-23 | 武汉乐神三宝蜂业有限公司 | Crease-resistant face cleaning honey of compound royal jelly and preparation method thereof |
| CN110613675A (en) * | 2019-10-28 | 2019-12-27 | 烟台华康海洋食品有限公司 | Formula and preparation process of sea cucumber facial cleanser |
| CN113425618A (en) * | 2020-03-23 | 2021-09-24 | 王福芳 | Composite active peptide facial cleanser and preparation method thereof |
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Application publication date: 20170111 |