CN106267141A - Anticoagulation antineoplastic polypeptide lyophilized powder capsule and preparation method thereof - Google Patents
Anticoagulation antineoplastic polypeptide lyophilized powder capsule and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of anticoagulation antineoplastic polypeptide lyophilized powder capsule and preparation method thereof, the implant of described polypeptide lyophilized powder capsule is mixed by tartary buckwheat extract lyophilized powder, sea cucumber intestine polypeptide lyophilized powder and bee pollen extract lyophilized powder 10:5:1 in mass ratio and forms；Described tartary buckwheat extract lyophilized powder is added the frequent autoclaving system of suitable quantity of water by the raw material of following weight portion, is filtered roguing and lyophilization acquisition: Black Radix Et Rhizoma Fagopyri Tatarici 50 60 parts, Herba bromi japonici 10 15 parts, Fructus Lycii 28 parts；The preparation method of described sea cucumber intestine polypeptide lyophilized powder comprises the steps: (1) Intestinum Stichopi japonici pretreatment；(2) homogenizing of Intestinum Stichopi japonici；(3) primary enzymolysis；(4) secondary enzymolysis；(5) ultrafiltration；(6) nanofiltration；(7) concentrate drying.Polypeptide lyophilized powder capsule rich in nutrition content prepared by the present invention, it is easy to absorb, absorption rate is high, and impurity and content of beary metal are low, are of high nutritive value, and can supplement the multiple nutrient coming from Intestinum Stichopi japonici and Radix Et Rhizoma Fagopyri Tatarici.
Description
Technical field
The present invention relates to a kind of anticoagulation antineoplastic polypeptide lyophilized powder capsule and preparation method thereof.
Background technology
Stichopus japonicus belongs to one of precious seafood, and it is internal contains more than the 50 kind of nutritional labeling useful to human physiological activity, wherein
Protein content is higher, trace element abundant species, it is possible to continuity human senility, tonifies Qi of the kidney, essence-replenishing and marrow-strengthening, allaying tiredness,
Have more anticoagulation, antitumor, antibacterial, antiviral and improve the effect of immunity.In recent years, the enterprise of processing sea cucumber the most more comes
The most, contained in the leftover bits and pieces-Intestinum Stichopi japonici during Holothurian machining nutritional labelings, no less than body wall.Its dry intestinal rate Han vanadium
It is 12 parts of million parts, higher than the rate Han vanadium in its body 3 times.It has effect of warming middle-Jiao and tonifying deficiency pain relieving, can treat stomach and ten
Two Duodenalulcers.
The utilization of Intestinum Stichopi japonici resource day by day causes attention, after Intestinum Stichopi japonici is manually removed sand by a lot of enterprises, cleans, cold air drying
Dry or lyophilization, then polishing or micronizing, make capsule product.But Intestinum Stichopi japonici is not done life by this kind of capsule product
Thing processes, and absorption rate is low, and nutritive value is had a greatly reduced quality.It addition, there is also in the process of raw material: manually going of Intestinum Stichopi japonici
Husky mud efficiency is low and silt is thorough, thorough with water cleaning, desalting, causes what content of beary metal and salinity in product exceeded standard to ask
Topic.
The elements such as iodine, calcium, phosphorus are the indispensable elements of human body, and Deficiency of Intake can affect healthy, and the present invention is by Intestinum Stichopi japonici
Extract obtain extract, then mix with other adjuvant, make polypeptide lyophilized powder capsule, have concurrently enrich the iodine, calcium, phosphorus function, also may be used
The multiple nutrient come from Intestinum Stichopi japonici is supplemented with efficient.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, it is provided that a kind of anticoagulation antineoplastic polypeptide lyophilized powder glue
Capsule and preparation method thereof, polypeptide lyophilized powder capsule rich in nutrition content prepared by the method, it is easy to absorb, impurity and a huge sum of money
Belong to content low, have concurrently enrich the iodine, calcium, phosphorus function, it is also possible to efficient supplement the multiple nutrient come from Intestinum Stichopi japonici.
The technical solution adopted for the present invention to solve the technical problems is:
Anticoagulation antineoplastic polypeptide lyophilized powder capsule, the implant of described polypeptide lyophilized powder capsule is frozen by tartary buckwheat extract
Dry powder, sea cucumber intestine polypeptide lyophilized powder and bee pollen extract lyophilized powder 10:5:1 in mass ratio mixing composition；
Described tartary buckwheat extract lyophilized powder by following weight portion raw material add the frequent autoclaving system of suitable quantity of water, filter roguing and
Lyophilization obtains: Black Radix Et Rhizoma Fagopyri Tatarici 50-60 part, Herba bromi japonici 10-15 part, Fructus Lycii 2-8 part；
The preparation method of described sea cucumber intestine polypeptide lyophilized powder comprises the steps:
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, pretreating agent A
For the water-insoluble glucan suspension of mass fraction 8-12%, described pretreating agent B is the reduction of mass fraction 0.5-1.5%
Type glutathion aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is
1:2-3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or
Pretreating agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, under room temperature at stirring
Reason 2-2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
Rich in silt, heavy metal, salinity in Intestinum Stichopi japonici, want to obtain the sea cucumber intestine polypeptide of high-quality, need fully to remove
Above-mentioned impurity, the design concept of the present invention is as follows: (1) water-insoluble glucan suspension is the aqueous suspension of macromole, divides greatly
Sub-glucosan itself has the effect of flocculation of reuniting, and can fully adsorb or the mud that flocculates in Intestinum Stichopi japonici and beavy metal impurity etc. are miscellaneous
The removal effect of matter, especially heavy metal is splendid, after using glucosan suspension pretreating agent pretreatment, and the stretching, extension of Intestinum Stichopi japonici
Property is the most excellent, and follow-up homogenizing, the effect of enzymolysis are substantially improved, it addition, glucosan safety non-toxic, the glucosan of residual has increasing on the contrary
Add immunity, improve effect of immunologic function, it is provided that the nutritive value of sea cucumber intestine polypeptide；(2) reduced glutathion aqueous solution
There is the function of good activating cell and tissue, the active oxygen in cell or tissue and osmotic pressure can be improved, maintain Stichopus japonicus
Enterocyte and the fresh and healthy state of tissue long period, be substantially improved the effect of follow-up ferment treatment, the safest nothing of glutathion
Poison, the glutathion of residual has the function improving immune system, removing toxic substances on the contrary, improves the nutritive value of sea cucumber intestine polypeptide, improves
The quality of finished product, reduces Heavy Metal Pollution risk that may be present in sea cucumber intestine polypeptide further or eliminates；It addition, residual
Reduced glutathion plays the effect of antioxidant, and polypeptide lyophilized powder capsule quality is stable, long shelf-life, edible safety；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer, remove the fat in Intestinum Stichopi japonici by lipase enzymolysis
Fat, can improve the quality of sea cucumber intestine polypeptide, extends the shelf-life of sea cucumber intestine polypeptide；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant, logical
Cross enzymolysis and the protein in Intestinum Stichopi japonici is fully degraded into aminoacid and micromolecule polypeptide, it is simple to the absorption of sea cucumber intestine polypeptide and profit
With, utilization rate is high, is of high nutritive value；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is for 150-300Da, by nanofiltration to sea cucumber intestine polypeptide desalting processing, it is thus achieved that sea cucumber intestine polypeptide salinity low, in good taste；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most chilled
It is dried to obtain powder sea cucumber intestine polypeptide.
Preferably, described tartary buckwheat extract lyophilized powder is added the frequent autoclaving system of suitable quantity of water, mistake by the raw material of following weight portion
Filter off miscellaneous and lyophilization acquisition: Black Radix Et Rhizoma Fagopyri Tatarici 55 parts, Herba bromi japonici 12 parts, Fructus Lycii 5 parts, amount of water is 2-3 times of raw materials quality, boiling
Temperature is 90-95 DEG C, and the time is 1-2 hour.
Preferably, in step (1), pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, described pre-
Inorganic agent B is the reduced glutathion aqueous solution of mass fraction 1.0%.
Preferably, temperature 35-65 DEG C of step (2) mesohigh homogenizing, pressure 120-400Mpa, circulates homogenizing at least two
Secondary.
Preferably, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is the 0.2-of fresh Intestinum Stichopi japonici quality
0.8%.
Preferably, in described step (4), the enzyme activity of compound protease and neutral protease is than for 2-3:1, and enzyme is total
Consumption is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
The preparation method of a kind of anticoagulation antineoplastic polypeptide lyophilized powder capsule, described preparation method is entered as follows
OK:
The first step: prepare sea cucumber intestine polypeptide lyophilized powder,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, pretreating agent A
For the water-insoluble glucan suspension of mass fraction 8-12%, described pretreating agent B is the reduction of mass fraction 0.5-1.5%
Type glutathion aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is
1:2-3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or
Pretreating agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, under room temperature at stirring
Reason 2-2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is 150-300Da；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most chilled
It is dried to obtain powder sea cucumber intestine polypeptide；
Second step, prepares tartary buckwheat extract and bee pollen extract lyophilized powder, will prepare each of tartary buckwheat extract lyophilized powder
Raw material mix homogeneously, adds water 2-3 times, in 90-95 DEG C, and boiling 1-2 hour, then remove large granular impurity, add after filtrate cooling
Entering the bee pollen extract of formula ratio, mix homogeneously is freeze-dried；
3rd step, prepares polypeptide lyophilized powder capsule finished product,
By the sea cucumber intestine polypeptide lyophilized powder of formula ratio, tartary buckwheat extract lyophilized powder and bee pollen extract lyophilized powder in proportion
Mix homogeneously, the most sterilized, fill obtains anticoagulation antineoplastic polypeptide lyophilized powder capsule.
The invention has the beneficial effects as follows: polypeptide lyophilized powder capsule rich in nutrition content prepared by the present invention, it is easy to absorb profit
High with, absorption rate, impurity and content of beary metal are low, are of high nutritive value, and can supplement and multiple come from Intestinum Stichopi japonici and Radix Et Rhizoma Fagopyri Tatarici
Nutrient, be eaten for a long time, there is significant anticoagulation antitumor efficacy.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail.
Embodiment 1:
Anticoagulation antineoplastic polypeptide lyophilized powder capsule, the implant of described polypeptide lyophilized powder capsule is frozen by tartary buckwheat extract
Dry powder, sea cucumber intestine polypeptide lyophilized powder and bee pollen extract lyophilized powder 10:5:1 in mass ratio mixing composition；
The preparation method of described sea cucumber intestine polypeptide lyophilized powder comprises the steps:
The first step, prepares sea cucumber intestine polypeptide, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A
Carrying out pretreatment with B, pretreating agent A is the water-insoluble glucan suspension of mass fraction 8%, and described pretreating agent B is matter
The reduced glutathion aqueous solution of amount mark 0.5%, concrete pretreatment operation is: first use pretreating agent A pre-under room temperature
Processing 2h, solid-liquid ratio is 1:2, then stirs pretreatment 2h under room temperature with pretreating agent B, then stands 45minh, and solid-liquid ratio is
1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, high pressure homogenize temperature 35-45 DEG C, pressure 120Mpa, circulation homogenizing 3 twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35 DEG C, adjusts pH value to 6.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3min, then cools down
Standing 1h to room temperature, remove supernatant oil layer, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is new fresh sea cucumber
The 0.2% of intestinal quality；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40 DEG C, adjusts pH value 6.5, adds appropriate
Complex food level protease hydrolyzed 2h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 10min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant, composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2:1, and the total consumption of enzyme is the 0.6% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-2000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-200Da；
(7) concentrate drying: it is 30% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder sea cucumber intestine polypeptide；
Second step, prepares tartary buckwheat extract and bee pollen extract lyophilized powder, will prepare each of tartary buckwheat extract lyophilized powder
Raw material mix homogeneously, adds water 2 times, in 90 DEG C, and boiling 2 hours, then remove large granular impurity, after filtrate cooling, add formula ratio
Bee pollen extract, mix homogeneously is freeze-dried；The each raw material of tartary buckwheat extract lyophilized powder consists of: Black Radix Et Rhizoma Fagopyri Tatarici 50
Part, Herba bromi japonici 10 parts, Fructus Lycii 2 parts；
3rd step, prepares polypeptide lyophilized powder capsule finished product,
By the sea cucumber intestine polypeptide lyophilized powder of formula ratio, tartary buckwheat extract lyophilized powder and bee pollen extract lyophilized powder in proportion
Mix homogeneously, the most sterilized, fill obtains anticoagulation antineoplastic polypeptide lyophilized powder capsule.
Embodiment 2
Anticoagulation antineoplastic polypeptide lyophilized powder capsule, the implant of described polypeptide lyophilized powder capsule is frozen by tartary buckwheat extract
Dry powder, sea cucumber intestine polypeptide lyophilized powder and bee pollen extract lyophilized powder 10:5:1 in mass ratio mixing composition；
The preparation method of described sea cucumber intestine polypeptide lyophilized powder comprises the steps:
The first step, prepares sea cucumber intestine polypeptide, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A
Carrying out pretreatment with B, pretreating agent A is the water-insoluble glucan suspension of mass fraction 12%, and described pretreating agent B is matter
The reduced glutathion aqueous solution of amount mark 1.5%, concrete pretreatment operation is: first use pretreating agent A pre-under room temperature
Processing 1h, solid-liquid ratio is 1:3, then stirs pretreatment 3h under room temperature with pretreating agent B, then stands 45min, and solid-liquid ratio is 1:
1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 60-65 DEG C of high pressure homogenize, pressure 400Mpa, circulation homogenizing twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 45 DEG C, adjusts pH value to 7.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.8% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；Multiple
Closing the enzyme activity of flavor protease and neutral protease ratio for 3:1, the total consumption of enzyme is the 1.0% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
200-300Da；
(7) concentrate drying: it is 40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder sea cucumber intestine polypeptide；
Second step, prepares tartary buckwheat extract and bee pollen extract lyophilized powder, will prepare each of tartary buckwheat extract lyophilized powder
Raw material mix homogeneously, adds water 3 times, in 95 DEG C, and boiling 1 hour, then remove large granular impurity, after filtrate cooling, add formula ratio
Bee pollen extract, mix homogeneously is freeze-dried；The each raw material of tartary buckwheat extract lyophilized powder consists of: Black Radix Et Rhizoma Fagopyri Tatarici 60
Part, Herba bromi japonici 15 parts, Fructus Lycii 8 parts；
3rd step, prepares polypeptide lyophilized powder capsule finished product,
By the sea cucumber intestine polypeptide lyophilized powder of formula ratio, tartary buckwheat extract lyophilized powder and bee pollen extract lyophilized powder in proportion
Mix homogeneously, the most sterilized, fill obtains anticoagulation antineoplastic polypeptide lyophilized powder capsule.
Embodiment 3:
Anticoagulation antineoplastic polypeptide lyophilized powder capsule, the implant of described polypeptide lyophilized powder capsule is frozen by tartary buckwheat extract
Dry powder, sea cucumber intestine polypeptide lyophilized powder and bee pollen extract lyophilized powder 10:5:1 in mass ratio mixing composition；
The preparation method of described sea cucumber intestine polypeptide lyophilized powder comprises the steps:
The first step, prepares sea cucumber intestine polypeptide, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A
Carrying out pretreatment with B, pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, and described pretreating agent B is matter
The reduced glutathion aqueous solution of amount mark 1.0%, concrete pretreatment operation is: by pretreating agent A and pretreating agent B with body
Long-pending ratio 1:1 mix homogeneously, is subsequently adding fresh Intestinum Stichopi japonici, and then stir process 2.5h under room temperature stands 1h, solid-liquid ratio 1:
2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 40-45 DEG C of high pressure homogenize, pressure 180Mpa, circulation homogenizing three times；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 40 DEG C, adjusts pH value to 7.0, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.5% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 45 DEG C, adjusts pH value 6.8, adds appropriate
Complex food level protease hydrolyzed 2.5h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 12mi n, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant；Composite flavor egg
The enzyme activity of white enzyme and neutral protease ratio is for 2.5:1, and the total consumption of enzyme is the 0.75% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.3 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
220Da；
(7) concentrate drying: it is 35% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder sea cucumber intestine polypeptide；
Second step, prepares tartary buckwheat extract and bee pollen extract lyophilized powder, will prepare each of tartary buckwheat extract lyophilized powder
Raw material mix homogeneously, adds water 2 times, in 95 DEG C, and boiling 1 hour, then remove large granular impurity, after filtrate cooling, add formula ratio
Bee pollen extract, mix homogeneously is freeze-dried；The each raw material of tartary buckwheat extract lyophilized powder consists of: Black Radix Et Rhizoma Fagopyri Tatarici 55
Part, Herba bromi japonici 12 parts, Fructus Lycii 5 parts；
3rd step, prepares polypeptide lyophilized powder capsule finished product,
By the sea cucumber intestine polypeptide lyophilized powder of formula ratio, tartary buckwheat extract lyophilized powder and bee pollen extract lyophilized powder in proportion
Mix homogeneously, the most sterilized, fill obtains anticoagulation antineoplastic polypeptide lyophilized powder capsule.
The quality index of the sea cucumber intestine polypeptide that embodiment 1-3 process prepares is examined by present inventor
Surveying, concrete outcome is as follows:
As it has been described above, sea cucumber intestine polypeptide amino acid nitrogen content is high, rich in multiple little molecule aminoacid and polypeptide, it is a kind of
Excellent functional additive, the anticoagulation antineoplastic polypeptide lyophilized powder capsule of the present invention includes that what Intestinum Stichopi japonici self contained has
The beneficial element that machine calcium constituent and Radix Et Rhizoma Fagopyri Tatarici contain, rich in nutrition content, also rich in several amino acids, small-molecular peptides, nutritive value
High, it is easy to absorbing, be eaten for a long time, anticoagulation antitumous effect is notable.
Embodiment described above is the one preferably scheme of the present invention, not makees the present invention any pro forma
Limit, on the premise of without departing from the technical scheme described in claim, also have other variant and remodeling.
Claims (7)
1. anticoagulation antineoplastic polypeptide lyophilized powder capsule, it is characterised in that: the implant of described polypeptide lyophilized powder capsule is by hardship
Buckwheat extract freeze-drying powder, sea cucumber intestine polypeptide lyophilized powder and bee pollen extract lyophilized powder 10:5:1 in mass ratio mixing composition；
Described tartary buckwheat extract lyophilized powder is added the frequent autoclaving system of suitable quantity of water by the raw material of following weight portion, is filtered roguing and freezing
It is dried and obtains: Black Radix Et Rhizoma Fagopyri Tatarici 50-60 part, Herba bromi japonici 10-15 part, Fructus Lycii 2-8 part；
The preparation method of described sea cucumber intestine polypeptide lyophilized powder comprises the steps:
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder sea cucumber intestine polypeptide lyophilized powder.
Anticoagulation antineoplastic polypeptide lyophilized powder capsule the most according to claim 1, it is characterised in that: described tartary buckwheat extract
Lyophilized powder is added the frequent autoclaving system of suitable quantity of water by the raw material of following weight portion, is filtered roguing and lyophilization acquisition: Black Radix Et Rhizoma Fagopyri Tatarici 55
Part, Herba bromi japonici 12 parts, Fructus Lycii 5 parts, amount of water is 2-3 times of raw materials quality, and brew temperatures is 90-95 DEG C, and the time is 1-2 hour.
Anticoagulation antineoplastic polypeptide lyophilized powder capsule the most according to claim 1, it is characterised in that: pre-in step (1)
Inorganic agent A is the water-insoluble glucan suspension of mass fraction 10%, and described pretreating agent B is going back of mass fraction 1.0%
Prototype glutathion aqueous solution.
Anticoagulation antineoplastic polypeptide lyophilized powder capsule the most according to claim 1, it is characterised in that: high in step (2)
Temperature 35-65 DEG C of pressure homogenizing, pressure 120-400Mpa, circulation homogenizing is at least twice.
Anticoagulation antineoplastic polypeptide lyophilized powder capsule the most according to claim 1, it is characterised in that: described food stage fat
The enzyme work of fat enzyme is 20,000 U/g, and enzyme dosage is the 0.4-0.8% of fresh Intestinum Stichopi japonici quality.
Anticoagulation antineoplastic polypeptide lyophilized powder capsule the most according to claim 1, it is characterised in that: described step (4)
The enzyme activity of middle compound protease and neutral protease is than for 2-3:1, and the total consumption of enzyme is the 0.6-of fresh Intestinum Stichopi japonici quality
1.0%.
7. a preparation method for anticoagulation antineoplastic polypeptide lyophilized powder capsule described in claim 1-6 any one, it is special
Levying and be, described preparation method is carried out as follows:
The first step: prepare sea cucumber intestine polypeptide lyophilized powder,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
(7) concentrate drying: it is 30-40% that nanofiltration concentrated solution is concentrated in vacuo to solid content further, the most freeze-dried
Obtain powder sea cucumber intestine polypeptide；
Second step, prepares tartary buckwheat extract and bee pollen extract lyophilized powder, will prepare each raw material of tartary buckwheat extract lyophilized powder
Mix homogeneously, adds water 2-3 times, in 90-95 DEG C, and boiling 1-2 hour, then remove large granular impurity, add after filtrate cooling and join
The bee pollen extract of side's amount, mix homogeneously is freeze-dried；
3rd step, prepares polypeptide lyophilized powder capsule finished product,
The sea cucumber intestine polypeptide lyophilized powder of formula ratio, tartary buckwheat extract lyophilized powder and bee pollen extract lyophilized powder are mixed in proportion
Uniformly, the most sterilized, fill obtains anticoagulation antineoplastic polypeptide lyophilized powder capsule.
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| CN106947797A (en) * | 2017-03-05 | 2017-07-14 | 赵德润 | A kind of rape pollen active peptide and preparation method thereof |
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