CN102697043A - Method for extracting amino acid nutrient solution from oyster meat of small reef oyster and product - Google Patents
Method for extracting amino acid nutrient solution from oyster meat of small reef oyster and product Download PDFInfo
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- CN102697043A CN102697043A CN2012101868718A CN201210186871A CN102697043A CN 102697043 A CN102697043 A CN 102697043A CN 2012101868718 A CN2012101868718 A CN 2012101868718A CN 201210186871 A CN201210186871 A CN 201210186871A CN 102697043 A CN102697043 A CN 102697043A
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Abstract
The invention discloses a method for extracting an amino acid nutrient solution from oyster meat of small reef oyster. The amino acid nutrient solution is prepared from the small reef oyster serving as a main raw material by pretreatment, soaking, homogenizing, enzymolysis, centrifuging, blending, filling, sterilization and packing. By determining the optimal soaking time, adopting a temporary ice water storage technology and combining and matching enzymes, the yield of the amino acid nutrient solution is higher than that in the single enzymolysis method in the prior art; and compared with the prior art, the invention has the advantages that the method is easy to operate and easy to control.
Description
Technical field
The present invention relates to a kind of method of from the oyster meat of the little oyster oyster of Hai Jiao, extracting amino-acid nutrition liquid, and the product that adopts this method to obtain.
Background technology
The little oyster oyster of Hai Jiao, mollusk has two shells, and one is little and flat, and another swells greatly, and the surface irregularity of shell colonizes on the Hai Jiao.Can be edible, oyster sauce can be obtained through refining again by delicious U.S.A, also can be used as medicine, and can control erysipelas, also cries oyster or oyster.
The little oyster oyster of Hai Jiao is compared with big oyster (living oyster), and volume is little, the oyster meat that includes; Also little a lot of and shelled peanut is almost big, this little oyster oyster nutritive value is much higher than livings oyster, generally is used for cooking congee or boiling soup; Be rich in protein, glycogen, fat and multivitamin, flavour delicate fragrance is delicious, no matter fry, boil, steam or as seasoning matter; Inlet is given birth to perfume invariably, leave a lasting and pleasant impression or aftertaste, and be rare flavouring.Dry product has tonifying liver heat-clearing, the enriching yin medical effectiveness such as beneficial essence that make eye bright, refresh oneself again, enjoys the good reputation of " marine milk ", is to nourish in the shellfish to be worth the highlyest, replenishes the calcium and zinc supplementation.
In the west, oyster is called as " milk in sea ", " sea fruit ", and oyster meat was once thought " among the extra large family your person " in ancient times, and this has affirmed fully the nutritive value of oyster.Oyster contains rich in protein, amino acid, also contains abundant vitamin such as VA, VB
1, VB
2, VB
3, VB
12, VC, VD
2, VH etc., contain inorganic salts and trace element in the oyster meat, human body is had the important physical effect.
Along with improving constantly of living standard, the change of people's diet structure, the food of pursuit nutrition height and health care has become people people's fashion.Therefore, big oyster aquatic products receive consumers in general's welcome deeply.Containing 19 kinds is the amino acid of needed by human, and taurine is the peculiar composition of oyster sauce, and is rich in 20 various trace elements such as zinc, is desirable strong kidney tonic nutrient article and health food of the male sex, favored by consumers in general, and market prospects are huge.
Summary of the invention
The object of the present invention is to provide a kind of method of from the oyster meat of the little oyster oyster of Hai Jiao, extracting amino-acid nutrition liquid, is primary raw material with the little oyster oyster of Hai Jiao, processes through pre-treatment, immersion, homogenate, enzymolysis, centrifugal, allotment, can, sterilization, packing.
Another object of the present invention is to provide a kind of amino-acid nutrition liquid that adopts method for preparing to obtain.
The present invention includes following steps:
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5-2.0% sodium chloride, 0.2-0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15-20min;
3), homogenate: oyster meat after will soaking drops into washes clean in the pure water, clean oyster meat and pure water are pressed the mixing of 1:0.5-1.0 weight ratio after, smash homogenate to pieces with bruisher, the rotating speed of homogenate is 10000-12000 rev/min, the time is 8-15 minute;
4), enzymolysis: the slurries after the collection homogenate; Add enzyme and carry out enzymolysis; Used enzyme is selected from one or both among Alcalase, PTN 6.0S, Protamex, Neutrase and the Papain; The consumption of enzyme is 0.1-0.2g enzyme/100g slurries, and hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour;
5), centrifugal: as the slurries behind the enzymolysis to be heated to 80 ℃, to let enzyme deactivation, use centrifuge centrifuging and taking supernatant then;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid through concentrating;
7), canned, pasteurization, pack finished product.
Oyster meat of the present invention is meant the software part of the little oyster oyster of Hai Jiao, i.e. the smart ovum part of the little oyster oyster of Hai Jiao.
In the pre-treatment step of the present invention, the temperature maintenance of frozen water is at 8-12 ℃, and the temporary time is 10-15 minute.
Be the technology of confirming that best frozen water is temporary, the inventor makes up a large amount of contrast work of having done to different processes, earlier part process conditions and result is listed:
Enzymolysis of the present invention; Used enzyme is the mixture of Alcalase or Protamex and PTN6.0S preferably; The consumption of enzyme is 0.1-0.2g enzyme/100g slurries, and wherein Alcalase or Protamex account for the 20-30% of the total consumption of enzyme, and PTN 6.0S accounts for the 70-80% of the total consumption of enzyme; Hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour.
Of the present invention centrifugal, rotating speed is 4500-5000 rev/min, and the time is 8-15 minute.
Carry out the technological effect of enzymolysis for explanation the present invention adopts different enzymes, done following technology contrast, concrete experiment condition and result are following:
Can find out that from above-mentioned experimental data the said method of the application is used through combination collocation that enzyme is carried out, make the yield of product than the height of single enzyme solution gained yield in the prior art.
The little oyster oyster of a kind of Hai Jiao amino-acid nutrition liquid adopts above-mentioned preparation method to obtain, and has following technical indicator:
The requirement of table 1 sense organ
| Project | Requirement |
| Color and luster | Colourless to sundown, impurity and suspension. |
| Flavour and smell | Tasteless |
| Form | Clear liquid does not have deposition |
| Impurity | The exogenous impurity that no twenty-twenty vision is visible |
Table 2 physical and chemical index
Table 3 microbiological indicator
| Project | Index |
| Total number of bacteria | <=100/mL |
| Escherichia coli | <=3/mL |
| Pathogenic bacteria | Must not detect |
The invention has the advantages that:
1, the present invention is through the combination of temporary time of soak time, frozen water temperature and frozen water in the little oyster oyster of the Hai Jiao process, and the factor that the fragmentation of oyster body, body fluid is outflowed, influences quality and oyster meat yield has been carried out better controlling, has obtained good effect;
2, the method for the invention is used through the combination collocation that enzyme is carried out; Make the yield of product than the height of single enzyme solution gained yield in the prior art; Compared with prior art, the said method of the application is simple to operate, easy to be controlled, and has improved the yield of product greatly;
3, the little oyster oyster of the Hai Jiao amino-acid nutrition liquid that obtains of the present invention; Contain rich in amino acid, can be directly use, can be used as also that other compositions of health food combine in healthy food material and the prior art as health food; Adopt prior art, prepare various forms of health foods;
3, through utilizing deep processing, improve product surcharge and competitiveness, promoted this area The development in society and economy the little oyster oyster of Hai Jiao.
The specific embodiment
The present invention will be described with embodiment below, but the present invention is not limited to these embodiment.
Embodiment 1
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 8 ℃ of frozen water temperature, temporary 15 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 5kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 8 minutes;
4), enzymolysis: collect the slurries after the homogenate, add the Alcalase enzyme and carry out enzymolysis, the consumption of enzyme is 0.1g enzyme/100g slurries, and hydrolysis temperature is 45 ℃, and the time is 8 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 7.0kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 2
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 12 ℃ of frozen water temperature, temporary 10 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 10kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 15 minutes;
4), enzymolysis: collect the slurries after the homogenate, add PTN 6.0S enzyme and carry out enzymolysis, the consumption of enzyme is 0.2g enzyme/100g slurries, and hydrolysis temperature is 55 ℃, and the time is 6 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 7.2kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 3
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 10 ℃ of frozen water temperature, temporary 15 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 8kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 8 minutes;
4), enzymolysis: collect the slurries after the homogenate, add the Protamex enzyme and carry out enzymolysis, the consumption of enzyme is 0.1g enzyme/100g slurries, and hydrolysis temperature is 45 ℃, and the time is 10 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 6.7kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 4
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 8 ℃ of frozen water temperature, temporary 12 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 6kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 15 minutes;
4), enzymolysis: collect the slurries after the homogenate, add the Neutrase enzyme and carry out enzymolysis, the consumption of enzyme is 0.2g enzyme/100g slurries, and hydrolysis temperature is 55 ℃, and the time is 6 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 6.3kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 5
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 12 ℃ of frozen water temperature, temporary 10 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 5kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 8 minutes;
4), enzymolysis: collect the slurries after the homogenate, add the Papain enzyme and carry out enzymolysis, the consumption of enzyme is 0.1g enzyme/100g slurries, and hydrolysis temperature is 50 ℃, and the time is 10 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 6.2kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 6
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 10 ℃ of frozen water temperature, temporary 15 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 10kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 10 minutes;
4), enzymolysis: the slurries after the collection homogenate; The mixture that adds Alcalase and PTN 6.0S enzyme, wherein Alcalase and PTN 6.0S account for 20% and 80% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.15g enzyme/100g slurries; Hydrolysis temperature is 50 ℃, and the time is 8 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.5kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 7
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 8 ℃ of frozen water temperature, temporary 12 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 8kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 12 minutes;
4), enzymolysis: the slurries after the collection homogenate; The mixture that adds Alcalase and PTN 6.0S enzyme, wherein Alcalase and PTN 6.0S account for 30% and 70% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.20g enzyme/100g slurries; Hydrolysis temperature is 45 ℃, and the time is 10 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.7kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 8
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 12 ℃ of frozen water temperature, temporary 10 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 6kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 8 minutes;
4), enzymolysis: the slurries after the collection homogenate; The mixture that adds Protamex and PTN 6.0S enzyme, wherein Protamex and PTN 6.0S account for 20% and 80% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.15g enzyme/100g slurries; Hydrolysis temperature is 50 ℃, and the time is 8 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.6kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 9
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice, 8 ℃ of frozen water temperature, temporary 15 minutes time with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
3), homogenate: the oyster meat after will soaking drops into washes clean in the pure water, after clean oyster meat 10kg and pure water 5kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 12 minutes;
4), enzymolysis: the slurries after the collection homogenate; The mixture that adds Protamex and PTN 6.0S enzyme, wherein Protamex and PTN 6.0S account for 30% and 70% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.20g enzyme/100g slurries; Hydrolysis temperature is 45 ℃, and the time is 10 hours;
5), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.4kg through concentrating;
7), canned, pasteurization, pack finished product.
Embodiment 10
The product that adopts embodiment 1-9 to obtain has following technical indicator:
The requirement of table 1 sense organ
| Project | Requirement |
| Color and luster | Colourless to sundown, impurity and suspension. |
| Flavour and smell | Tasteless |
| Form | Clear liquid does not have deposition |
| Impurity | The exogenous impurity that no twenty-twenty vision is visible |
Table 2 physical and chemical index
Table 3 microbiological indicator
| Project | Index |
| Total number of bacteria | <=100/mL |
| Escherichia coli | <=3/mL |
| Pathogenic bacteria | Must not detect |
Claims (6)
1. from the oyster meat of the little oyster oyster of Hai Jiao, extract the method for amino-acid nutrition liquid, comprise the steps:
1), pre-treatment: collect the fresh little oyster oyster of Hai Jiao oyster meat, remove residual shell, clean up, oyster meat is temporarily stored in the frozen water that has added trash ice with pure water;
2), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5-2.0% sodium chloride, 0.2-0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15-20min;
3), homogenate: oyster meat after will soaking drops into washes clean in the pure water, clean oyster meat and pure water are pressed the mixing of 1:0.5-1.0 weight ratio after, smash homogenate to pieces with bruisher, the rotating speed of homogenate is 10000-12000 rev/min, the time is 8-15 minute;
4), enzymolysis: the slurries after the collection homogenate; Add enzyme and carry out enzymolysis; Used enzyme is selected from one or both among Alcalase, PTN 6.0S, Protamex, Neutrase and the Papain; The consumption of enzyme is 0.1-0.2g enzyme/100g slurries, and hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour;
5), centrifugal: as the slurries behind the enzymolysis to be heated to 80 ℃, to let enzyme deactivation, use centrifuge centrifuging and taking supernatant then;
6), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid through concentrating;
7), canned, pasteurization, pack finished product.
2. the method for from the oyster meat of the little oyster oyster of Hai Jiao, extracting amino-acid nutrition liquid according to claim 1, it is characterized in that: oyster meat is meant the software part of the little oyster oyster of Hai Jiao, i.e. the smart ovum part of the little oyster oyster of Hai Jiao.
3. the method for from the oyster meat of the little oyster oyster of Hai Jiao, extracting amino-acid nutrition liquid according to claim 1 is characterized in that: in the described pre-treatment step, the temperature maintenance of frozen water is at 8-12 ℃, and the temporary time is 10-15 minute.
4. the method for from the oyster meat of the little oyster oyster of Hai Jiao, extracting amino-acid nutrition liquid according to claim 1; It is characterized in that: described enzymolysis, used enzyme are the mixture of Alcalase or Protamex and PTN 6.0S, and the consumption of enzyme is 0.1-0.2g enzyme/100g slurries; Wherein Alcalase or Protamex account for the 20-30% of the total consumption of enzyme; PTN 6.0S accounts for the 70-80% of the total consumption of enzyme, and hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour.
5. the method for from the oyster meat of the little oyster oyster of Hai Jiao, extracting amino-acid nutrition liquid according to claim 1, it is characterized in that: described centrifugal, rotating speed is 4500-5000 rev/min, and the time is 8-15 minute.
6. the little oyster oyster of the Hai Jiao amino-acid nutrition liquid that each said method makes in the claim 1～6.
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| CN104664536A (en) * | 2015-03-31 | 2015-06-03 | 广西还珠海洋生物科技有限公司 | Nutrient solution of small oyster in ocean reef and preparation method of nutrient solution |
| CN104687057A (en) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | Small reef oyster de-alcoholic medicine and preparation method thereof |
| CN105326035A (en) * | 2015-10-19 | 2016-02-17 | 集美大学 | Production method of low-salt oyster polypeptide and oligosaccharide nutrition powder |
| CN105326035B (en) * | 2015-10-19 | 2018-12-04 | 集美大学 | A kind of production method of less salt oyster polypeptide and oligosaccharide nutrient powder |
| CN106235091A (en) * | 2016-08-16 | 2016-12-21 | 广西还珠海洋生物科技有限公司 | Sea reef little oyster oyster liquid and preparation method thereof |
| CN106262032A (en) * | 2016-08-16 | 2017-01-04 | 广西还珠海洋生物科技有限公司 | Sea reef little oyster oyster liquid and preparation technology thereof |
| CN106262015A (en) * | 2016-08-16 | 2017-01-04 | 广西还珠海洋生物科技有限公司 | A kind of sea reef little oyster oyster liquid and preparation method thereof |
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Application publication date: 20121003 |