CN104172353A - Giant salamander polypeptide beverage and preparation method thereof - Google Patents
Giant salamander polypeptide beverage and preparation method thereof Download PDFInfo
- Publication number
- CN104172353A CN104172353A CN201410404611.2A CN201410404611A CN104172353A CN 104172353 A CN104172353 A CN 104172353A CN 201410404611 A CN201410404611 A CN 201410404611A CN 104172353 A CN104172353 A CN 104172353A
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- Prior art keywords
- giant salamander
- liquid
- polypeptide
- salamander polypeptide
- beverage
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Links
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 149
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 149
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 131
- 235000013361 beverage Nutrition 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 241000157862 Dicamptodontidae Species 0.000 title claims abstract 38
- 239000007788 liquid Substances 0.000 claims abstract description 87
- 239000000796 flavoring agent Substances 0.000 claims abstract description 31
- 235000019634 flavors Nutrition 0.000 claims abstract description 31
- 235000000346 sugar Nutrition 0.000 claims abstract description 30
- 235000015203 fruit juice Nutrition 0.000 claims abstract description 28
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 26
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 26
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 26
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 26
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 26
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims abstract description 23
- 235000002906 tartaric acid Nutrition 0.000 claims abstract description 23
- 239000011975 tartaric acid Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 73
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 57
- 239000004365 Protease Substances 0.000 claims description 46
- 241000218628 Ginkgo Species 0.000 claims description 42
- 235000011201 Ginkgo Nutrition 0.000 claims description 42
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 42
- 235000013372 meat Nutrition 0.000 claims description 42
- 238000001914 filtration Methods 0.000 claims description 35
- 102000035195 Peptidases Human genes 0.000 claims description 32
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- 235000019419 proteases Nutrition 0.000 claims description 32
- 238000001802 infusion Methods 0.000 claims description 29
- 239000002994 raw material Substances 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 20
- 108090000790 Enzymes Proteins 0.000 claims description 20
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 19
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 19
- 239000001630 malic acid Substances 0.000 claims description 19
- 235000011090 malic acid Nutrition 0.000 claims description 19
- 239000012535 impurity Substances 0.000 claims description 18
- 229940088598 enzyme Drugs 0.000 claims description 16
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- 102000004400 Aminopeptidases Human genes 0.000 claims description 14
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- 239000000470 constituent Substances 0.000 claims description 13
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
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- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 10
- 229940067866 dandelion extract Drugs 0.000 claims description 10
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
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- 239000001845 taraxacum officinale leaf extract Substances 0.000 claims description 10
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- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 4
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- 239000001116 FEMA 4028 Substances 0.000 abstract 1
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 abstract 1
- 235000011175 beta-cyclodextrine Nutrition 0.000 abstract 1
- 229960004853 betadex Drugs 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 229940082509 xanthan gum Drugs 0.000 abstract 1
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- 210000004185 liver Anatomy 0.000 description 6
- 235000020776 essential amino acid Nutrition 0.000 description 5
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- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
- 239000004472 Lysine Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000009849 deactivation Effects 0.000 description 4
- 235000021552 granulated sugar Nutrition 0.000 description 4
- 235000019534 high fructose corn syrup Nutrition 0.000 description 4
- 239000011435 rock Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 239000002932 luster Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- ORQIZUYAGXZVPI-LBPRGKRZSA-N (5s)-2-methyl-5-[5-(2-methylpropyl)furan-3-yl]cyclohex-2-en-1-one Chemical compound O1C(CC(C)C)=CC([C@@H]2CC(=O)C(C)=CC2)=C1 ORQIZUYAGXZVPI-LBPRGKRZSA-N 0.000 description 2
- 241000395633 Andrias japonicus Species 0.000 description 2
- 102000008934 Muscle Proteins Human genes 0.000 description 2
- 108010074084 Muscle Proteins Proteins 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
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- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241001482311 Trionychidae Species 0.000 description 1
- HZYXFRGVBOPPNZ-UHFFFAOYSA-N UNPD88870 Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)=CCC(CC)C(C)C)C1(C)CC2 HZYXFRGVBOPPNZ-UHFFFAOYSA-N 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 235000015197 apple juice Nutrition 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- LGJMUZUPVCAVPU-UHFFFAOYSA-N beta-Sitostanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CC)C(C)C)C1(C)CC2 LGJMUZUPVCAVPU-UHFFFAOYSA-N 0.000 description 1
- ORQIZUYAGXZVPI-UHFFFAOYSA-N bilobanone Natural products O1C(CC(C)C)=CC(C2CC(=O)C(C)=CC2)=C1 ORQIZUYAGXZVPI-UHFFFAOYSA-N 0.000 description 1
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- 230000002496 gastric effect Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 231100000753 hepatic injury Toxicity 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
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- HCXVJBMSMIARIN-PHZDYDNGSA-N stigmasterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)/C=C/[C@@H](CC)C(C)C)[C@@]1(C)CC2 HCXVJBMSMIARIN-PHZDYDNGSA-N 0.000 description 1
- 229940032091 stigmasterol Drugs 0.000 description 1
- BFDNMXAIBMJLBB-UHFFFAOYSA-N stigmasterol Natural products CCC(C=CC(C)C1CCCC2C3CC=C4CC(O)CCC4(C)C3CCC12C)C(C)C BFDNMXAIBMJLBB-UHFFFAOYSA-N 0.000 description 1
- 235000016831 stigmasterol Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000001333 taraxacum officinale rhizomes root extract Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 235000020097 white wine Nutrition 0.000 description 1
- 235000005765 wild carrot Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a beverage or a health beverage, in particular relates to a giant salamander polypeptide beverage and a preparation method thereof, and belongs to the technical fields of giant salamander deep processing, food, health products and medicines. The giant salamander polypeptide beverage comprises 5%-15% of sugar, 0.05%-0.15% of beta-cyclodextrin, 0.05%-0.15% of xanthan gum, 0.01%-0.025% of potassium sorbate, 0.15%-0.4% of tartaric acid, 3%-10% of fruit juice and the balance being a giant salamander polypeptide liquid. The giant salamander polypeptide beverage is uniformly golden yellow or pale yellow, looks like a semitransparent uniform liquid, is free of the peculiar unpleasant smell of giant salamander polypeptide by use of the components of the formula, and has the advantages of good taste and flavor, high nutritional value, environmental friendliness and safety, and the good-quality giant salamander polypeptide can be supplemented conveniently in daily life.
Description
Technical field
The present invention relates to a kind of drink or health beverages, especially a kind of giant salamander polypeptide beverage and preparation method thereof, belongs to giant salamander deep processing and food, health products and medical technical field.
Background technology
Giant salamander is the high animal of a kind of edibility, its fine and tender taste, delicious flavour, contain good protein, abundant amino acid and trace element, nutritive value is high, is described as " ginseng in water ", and giant salamander muscle protein is a kind of high-quality protein, essential amino acids content is high, proportion of composing is good, meets human body requirement pattern completely, and its nutritive value is much better than abalone, bird's nest, shark's fin and soft-shelled turtle.Giant salamander muscle protein is rich in 18 seed amino acids, wherein 6 kinds are taste amino acid and account for 42.77% of total amino acid content, 8 kinds of essential amino acids account for 40.72% of total amino acid content, and essential amino acid and nonessential amino acid ratio are 68.68%, all meet the idealized model of FAO/WHO; The scoring of giant salamander essential amino acid is high, meets human body requirement pattern degree quite high, wherein contains the lysine easily lacking in abundant China's staple food, can be reasonably combined edible with staple food, and to play protein complementary action.
The eating method of artificially-cultured giant salamander is mainly the mode that adopts conventional cooking, is limited to the digestion desorption system of human body, and the nutritive value of giant salamander can not be fully utilized.Along with the development of biotechnology, occur giant salamander to carry out deep process.
Summary of the invention
Goal of the invention of the present invention is: what a kind of giant salamander polypeptide is provided was raw material has health care drink, improve the absorption of giant salamander polypeptide, this drink has unique local flavor and mouthfeel simultaneously, can overcome giant salamander polypeptide and have foreign odor, can be accepted by consumers in general.
The technical solution used in the present invention is as follows:
A kind of giant salamander polypeptide beverage, comprise giant salamander polypeptide liquid, sugar, beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid and fruit juice, each constituent mass mark is sugared 5-15%, beta-schardinger dextrin-0.05-0.15%, xanthans 0.05-0.15%, potassium sorbate 0.01-0.025%, tartaric acid 0.15-0.4%, fruit juice 3-10%, and all the other are giant salamander polypeptide liquid.
In this giant salamander polypeptide beverage, also comprise the ginkgo extract of 0.5-5% and the dandelion extract of 0.5-1%.
Described tartaric acid is made up of taking mass ratio as 3-6:1 citric acid and malic acid, and described fruit juice is cider; Described fruit juice is for being Passion Fruit Juice.
Each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthans 0.1%, potassium sorbate 0.02%, citric acid 0.25%, malic acid 0.05%, cider 5% in giant salamander polypeptide beverage, all the other are giant salamander polypeptide liquid.
Each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthans 0.1%, potassium sorbate 0.02%, citric acid 0.25%, malic acid 0.05%, white fragrant fruit juice 5% in giant salamander polypeptide beverage, all the other are giant salamander polypeptide liquid.
The each constituent mass mark of giant salamander polypeptide beverage sugar 10%, beta-schardinger dextrin-1%, xanthans 0.1%, potassium sorbate 0.02%, citric acid 0.25%, malic acid 0.05%, white fragrant fruit juice 5%, ginkgo extract 3%, dandelion extract 0.6%, all the other are giant salamander polypeptide liquid.
In described giant salamander polypeptide beverage, content of peptides is not less than 0.02%, and surplus is water.
The molecular weight of described giant salamander polypeptide liquid is not more than 20000Da.
A preparation method for giant salamander polypeptide beverage, comprises following steps:
(1) raw material sugar is dissolved in water saccharogenesis water filtration removal of impurities;
(2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid are incorporated to stirring and dissolving in appropriate water, add again syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, control content of peptides in giant salamander polypeptide beverage and be not less than 0.02%;
(3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid;
(4) product liquid being carried out to filling and sterilization forms.
It is emptied the intestine work giant salamander a couple of days of stopping eating, then slaughter gall-bladder, tissue is for the preparation of giant salamander polypeptide entirely.
A kind of preparation method of giant salamander polypeptide beverage, can also be in step (2), beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid, ginkgo extract and dandelion extract are incorporated to stirring and dissolving in appropriate water, add again syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, control content of peptides in giant salamander polypeptide beverage and be not less than 0.02%.
Described giant salamander polypeptide liquid makes as follows:
(a) pretreatment: giant salamander is cleaned and stripping and slicing;
(b) infusion: add water to gained giant salamander cube meat, infusion is to giant salamander cube meat organization softening;
(c) enzymolysis: after feed liquid after infusion is cooling, moisturizing regulates solid-liquid ratio, add compound protease to carry out enzymolysis, described compound protease comprises neutral proteinase, papain and flavor protease composition again, and described flavor protease is made up of aminopeptidase and carboxypeptidase;
(d) enzyme that goes out: after enzymolysis completes, enzymolysis liquid is boiled to the enzyme that goes out;
(e) filter: the feed liquid of going out after enzyme is carried out to multistage filtering, the pure polypeptide products of system.
In described step (a), giant salamander is cut into 0.5-2cm
3piece.
In described step (c), described neutral protein enzyme dosage is the 0.5%-1.5% of giant salamander cube meat quality, and papain consumption is the 0.6%-2.0% of giant salamander cube meat quality; Described flavor protease is made up of with mass ratio 3-5:1 aminopeptidase and carboxypeptidase, and flavor protease consumption is the 0.3-1.0% of giant salamander cube meat quality.Preferably, described neutral protein enzyme dosage is 1.2% of giant salamander cube meat quality, papain consumption is 1.6% of giant salamander cube meat quality, and described flavor protease is made up of with mass ratio 4:1 aminopeptidase and carboxypeptidase, and flavor protease consumption is 0.8% of giant salamander cube meat quality.Regulate in solid-liquid ratio in described step (c) moisturizing, regulating the ratio of giant salamander cube meat and water is 1:2-6, adopts the mode stirring to carry out enzymolysis, and mixing speed is 400r/min, hydrolysis temperature is that 45 DEG C-55 DEG C, enzymolysis time are 4-8 hour, and enzymolysis PH is 6.5-7.5.
In described step in (e), adopt three grades of step apertures to filter, 30-50 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is that the milipore filter of 20000Da carries out ultrafiltration.
Described giant salamander polypeptide liquid can also make as follows:
(a) giant salamander cube meat is cleaned, stripping and slicing, adds the aseptic ultra-pure water of 3 times of volumes, makes homogenate, then adds the aseptic ultra-pure water of 3-5 times of volume, mixes;
(b) regulate the pH value of homogenate to 3-4, in-30 DEG C～-10 DEG C multigelations 3-8 time, the homogenate after last freeze thawing, 5-10 DEG C of centrifugal and filtration that is 6000r/min through 2 times, each 10-15 minute, rotating speed, is got to supernatant;
(c) regulate supernatant pH value to 5.5-6.5, filter with milipore filter, obtain giant salamander polypeptide liquid.
In described step (b), for the first time after centrifugal filtration by supernatant in 60-70 DEG C heating 10 minutes, be cooled to room temperature, then carry out centrifugal for the second time.
In described step (c), adopt three grades of step apertures to filter, 30-50 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is that the milipore filter of 10000Da carries out ultrafiltration.
The preparation technology of the giant salamander polypeptide liquid of preparation is simple and stable like this, through multigelation, makes clasmatosis, and then discharges polypeptide more.Adopt pure physical operations preparation, do not add other composition, purer more healthy, and the activity that can preserve to greatest extent polypeptide.Adopt twice centrifugal and filtration, coordinate milipore filter, can obtain being not more than the polypeptide extract of 10000Da, be more conducive to absorption of human body.
The preparation method of described Passion Fruit Juice is:
(1) select more than 95% well-done passion fruit, remove after pericarp, obtain passion fruit pulp, then add appropriate water and in temperature be under the condition of 80-95 DEG C and carry out infusion 30 minutes, then filter, then at vacuum 0.08Mpa, temperature is vacuum impregnation 25min under the condition of 60 DEG C, under normal pressure, flood 2-4 hour again, obtain passion fruit Normal juice;
(2) in the weight portion of passion fruit pulp, rock sugar 20-40 part, HFCS 5-30 part, brown sugar 0.5-1.5 part and water 30-150 part are put in pot and is heated to 70-90 DEG C, be stirred to completely and melt;
(3) solution obtaining in the passion fruit Normal juice of gained in step (1) and step (2) is mixed, obtain Passion Fruit Juice.In weight portion, 150 parts, water in 80 parts of passion fruit pulp, step (1), rock sugar 20-40 part, HFCS 5-30 part, brown sugar 0.5-1.5 part, the middle water 30-150 part of step (2).
The preparation method of described ginkgo extract is:
(1) get fresh ginkgo leaf, then make its air drying 3-5 days under field conditions (factors), then it is added water in iron pan carry out infusion, the temperature of infusion is 80-95 DEG C, and the time is 20-50 minute, then filters; Obtain the extract of ginkgo leaf; In weight portion, fresh ginkgo leaf is 50～100 parts; The addition of water is 200-300 part.
(2) get commercially available ginkgo fruit, make, after its fragmentation, fruit was pulverized to 200 mesh sieves, obtain ginkgo pulp; Then together with the shell of ginkgo fruit being added water with the leaf slag of the ginkgo leaf obtaining in step (1), carry out infusion, the temperature of infusion is 90-95 DEG C, and the time is 3-5h, then filters, and obtains filtrate; The weight portion of ginkgo fruit is 20-40 part, and the weight portion of water is 120-160 part.
(3) filtrate that obtains in the filtrate obtaining in step (1) and step (2) is mixed, then add the ginkgo fruit of pulverizing, and white granulated sugar mixes, obtain ginkgo extract.In weight portion, the weight portion of white granulated sugar is 30-60 part.
Described dandelion extract dandelion root fluidextract.
In sum, owing to having adopted technique scheme, the invention has the beneficial effects as follows: giant salamander polypeptide beverage nutritive value of the present invention is abundant, can utilize each component of formula itself to overcome the special foreign odor of giant salamander polypeptide, there is mouthfeel and raciness, be of high nutritive value, green safety, facilitate the daily high-quality polypeptide to giant salamander to supplement; Adopt the giant salamander polypeptide of described method system, can effectively avoid giant salamander horny layer of epidermis on the impact of enzymolysis process and enzymolysis product, enzymatic hydrolysis condition gentleness, can carry out sufficient enzymolysis to Megalobatrachus japonicus daoidianuas(Blanchard), and enzymolysis process is easy to control, enzymolysis product safety and be beneficial to absorption of human body.The giant salamander polypeptide beverage of preparing with it can relieve the effect of alcohol, protects the liver, diuresis, strengthening the spleen and stomach.
Detailed description of the invention
Disclosed all features in this description, or step in disclosed all methods or process, except mutually exclusive feature and/or step, all can combine by any way.
Disclosed arbitrary feature in this description, unless narration especially all can be replaced by other equivalences or the alternative features with similar object.,, unless narration especially, each feature is an example in a series of equivalences or similar characteristics.It is emptied the intestine work giant salamander a couple of days of stopping eating, then slaughter gall-bladder, tissue is for the preparation of giant salamander polypeptide entirely.
Embodiment 1
Giant salamander polypeptide preparation: (a) pretreatment: giant salamander is cleaned and is cut into 0.5-2cm
3bulk (b) infusion: add water to gained giant salamander cube meat, infusion 20min-30min at 111 DEG C-123 DEG C, to giant salamander cube meat organization softening, (c) enzymolysis: feed liquid after infusion with pressure naturally cooling after, regulate material-water ratio as 1:2 taking the quality moisturizing of raw material giant salamander cube meat, add again by neutral proteinase, the compound protease of papain and flavor protease composition carries out enzymolysis, described neutral proteinase is 50,000 U/g, consumption is 0.5% of giant salamander cube meat quality, papain is 100,000 U/g, consumption is 0.6% of giant salamander cube meat quality, flavor protease is made up of with mass ratio 3:1 aminopeptidase and carboxypeptidase, aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption is 0.3% of giant salamander cube meat quality, enzymolysis adopts the mode stirring to carry out, mixing speed is 400r/min, hydrolysis temperature is 45 DEG C, enzymolysis time is 4 hours, enzymolysis is to carry out for 6.5 times at PH, (d) enzyme that goes out: after enzymolysis completes, enzymolysis liquid is boiled to deactivation 15min at 90 DEG C-100 DEG C, (e) filter: the feed liquid of going out after enzyme is carried out to multistage filtering, first adopt three grades of step apertures to filter, 30 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is the pure polypeptide products that the milipore filter of 20000Da carries out ultrafiltration system.
The preparation of giant salamander polypeptide beverage: (1) is by the saccharogenesis water filtration removal of impurities that is dissolved in water of raw material sugar; (2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid are incorporated to stirring and dissolving in appropriate water, then add syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide beverage is 0.02%; (3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid; (4) product liquid being carried out to filling and sterilization forms.
Wherein: described tartaric acid is made up of taking mass ratio as 3-6:1 citric acid and malic acid.Described fruit juice is cider; Each constituent mass mark is raw material sugar 5%, beta-schardinger dextrin-0.05%, xanthans 0.05%, potassium sorbate 0.01%, citric acid 0.12%, malic acid 0.03%, cider 3%, all the other are giant salamander polypeptide liquid, in preparation, control content of peptides in giant salamander polypeptide beverage by adding water, water can come from giant salamander polypeptide liquid or raw material components dissolves water.
Embodiment 2
Giant salamander polypeptide preparation: (a) pretreatment: giant salamander is cleaned and is cut into 0.5-2cm
3bulk, (b) infusion: add water to gained giant salamander cube meat, infusion 20min-30min at 111 DEG C-123 DEG C, to giant salamander cube meat organization softening, (c) enzymolysis: feed liquid after infusion with pressure naturally cooling after, regulate material-water ratio as 1:4 taking the quality moisturizing of raw material giant salamander cube meat, add again by neutral proteinase, the compound protease of papain and flavor protease composition carries out enzymolysis, described neutral proteinase is 50,000 U/g, consumption is 1.2% of giant salamander cube meat quality, papain is 100,000 U/g, consumption is 1.6% of giant salamander cube meat quality, flavor protease is made up of with mass ratio 5:1 aminopeptidase and carboxypeptidase, aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption is 1.0% of giant salamander cube meat quality, enzymolysis adopts the mode stirring to carry out, mixing speed is 400r/min, hydrolysis temperature is 50 DEG C, enzymolysis time is 6 hours, enzymolysis is to carry out for 7.0 times at PH, (d) enzyme that goes out: after enzymolysis completes, enzymolysis liquid is boiled to the deactivation 15min enzyme that goes out at 90 DEG C-100 DEG C, (e) filter: the feed liquid of going out after enzyme is carried out to multistage filtering, first adopt three grades of step apertures to filter, 40 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is the pure polypeptide products that the milipore filter of 20000Da carries out ultrafiltration system.
The preparation of giant salamander polypeptide beverage: (1) is by the saccharogenesis water filtration removal of impurities that is dissolved in water of raw material sugar; (2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid are incorporated to stirring and dissolving in appropriate water, then add syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide beverage is 3%; (3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid; (4) product liquid being carried out to filling and sterilization forms.
Wherein: described tartaric acid is made up of taking mass ratio as 5:1 citric acid and malic acid.Described fruit juice is cider; Each constituent mass mark is raw material sugar 10%, beta-schardinger dextrin-1%, xanthans 0.1%, potassium sorbate 0.02%, citric acid 0.25%, malic acid 0.05%, cider 5%, all the other are giant salamander polypeptide liquid, in preparation, control content of peptides in giant salamander polypeptide beverage by adding water, water can come from giant salamander polypeptide liquid or raw material components dissolves water.
Embodiment 3
Giant salamander polypeptide preparation: (a) pretreatment: giant salamander is cleaned and is cut into 0.5-2cm
3bulk, (b) infusion: add water to gained giant salamander cube meat, infusion 20min-30min at 111 DEG C-123 DEG C, to giant salamander cube meat organization softening, (c) enzymolysis: feed liquid after infusion with pressure naturally cooling after, regulate material-water ratio as 1:6 taking the quality moisturizing of raw material giant salamander cube meat, add again by neutral proteinase, the compound protease of papain and flavor protease composition carries out enzymolysis, described neutral proteinase is 50,000 U/g, consumption is 1.5% of giant salamander cube meat quality, papain is 100,000 U/g, consumption is 2.0% of giant salamander cube meat quality, flavor protease is made up of with mass ratio 4:1 aminopeptidase and carboxypeptidase, aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption is 0.8% of giant salamander cube meat quality, enzymolysis adopts the mode stirring to carry out, mixing speed is 400r/min, hydrolysis temperature is 55 DEG C, enzymolysis time is 8 hours, enzymolysis is to carry out for 7.5 times at PH, (d) enzyme that goes out: after enzymolysis completes, enzymolysis liquid is boiled to the deactivation 15min enzyme that goes out at 90 DEG C-100 DEG C, (e) filter: the feed liquid of going out after enzyme is carried out to multistage filtering, first adopt three grades of step apertures to filter, 50 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is the pure polypeptide products that the milipore filter of 20000Da carries out ultrafiltration system.
The preparation of giant salamander polypeptide beverage: (1) is by the saccharogenesis water filtration removal of impurities that is dissolved in water of raw material sugar; (2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid are incorporated to stirring and dissolving in appropriate water, then add syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide beverage is 3.5%; (3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid; (4) product liquid being carried out to filling and sterilization forms.
Wherein: described tartaric acid is made up of taking mass ratio as 3:1 citric acid and malic acid.Described fruit juice is cider; Each constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthans 0.15%, potassium sorbate 0.025%, citric acid 0.3%, malic acid 0.1%, apple juice 10%, all the other are giant salamander polypeptide liquid, in preparation, control content of peptides in giant salamander polypeptide beverage by adding water, water can come from giant salamander polypeptide liquid or raw material components dissolves water.
In the above-described embodiments, in the preparation process of giant salamander polypeptide beverage (4), adopt ultrahigh-pressure sterilization, 121 DEG C of temperature, pressure 0.11Mpa, sterilizing time 10min.
The giant salamander polypeptide beverage obtaining in embodiment 1-embodiment 3 is analyzed, the giant salamander polypeptide beverage color and luster obtaining in embodiment 1-embodiment 3 is the golden yellow or light yellow of uniformity, the be translucent liquid of uniformity of outward appearance, has cider and the intrinsic fragrance of giant salamander enzymolysis liquid and flavour body.
Embodiment 4:
Giant salamander polypeptide preparation: (a) pretreatment: giant salamander is cleaned and is cut into 0.5-2cm
3bulk, (b) infusion: add water to gained giant salamander cube meat, infusion 20min-30min at 111 DEG C-123 DEG C, to giant salamander cube meat organization softening, (c) enzymolysis: feed liquid after infusion with pressure naturally cooling after, regulate material-water ratio as 1:2 taking the quality moisturizing of raw material giant salamander cube meat, add again by neutral proteinase, the compound protease of papain and flavor protease composition carries out enzymolysis, described neutral proteinase is 50,000 U/g, consumption is 0.5% of giant salamander cube meat quality, papain is 100,000 U/g, consumption is 0.6% of giant salamander cube meat quality, flavor protease is made up of with mass ratio 3:1 aminopeptidase and carboxypeptidase, aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption is 0.3% of giant salamander cube meat quality, enzymolysis adopts the mode stirring to carry out, mixing speed is 400r/min, hydrolysis temperature is 45 DEG C, enzymolysis time is 4 hours, enzymolysis is to carry out for 6.5 times at PH, (d) enzyme that goes out: after enzymolysis completes, enzymolysis liquid is boiled to deactivation 15min at 90 DEG C-100 DEG C, (e) filter: the feed liquid of going out after enzyme is carried out to multistage filtering, first adopt three grades of step apertures to filter, 30 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is the pure polypeptide products that the milipore filter of 20000Da carries out ultrafiltration system.
The preparation of giant salamander polypeptide beverage: (1) is by the saccharogenesis water filtration removal of impurities that is dissolved in water of raw material sugar; (2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid, ginkgo extract and Passion Fruit Juice are incorporated to stirring and dissolving in appropriate water, add again syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide beverage is 0.35%; (3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid; (4) product liquid being carried out to filling and sterilization forms.
Wherein: described tartaric acid is made up of taking mass ratio as 3:1 citric acid and malic acid.Described fruit juice is Passion Fruit Juice; Each constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthans 0.15%, potassium sorbate 0.025%, citric acid 0.3%, malic acid 0.1%, Passion Fruit Juice 10%, ginkgo extract 3%, dandelion extract 0.6%, all the other are giant salamander polypeptide liquid, in preparation, control content of peptides in giant salamander polypeptide beverage by adding water, water can come from giant salamander polypeptide liquid or raw material components dissolves water.
In the above-described embodiments, in the preparation process of giant salamander polypeptide beverage (4), adopt ultrahigh-pressure sterilization, 121 DEG C of temperature, pressure 0.11Mpa, sterilizing time 10min.
Adopt the giant salamander polypeptide beverage color and luster of system of the present invention to be the golden yellow or light yellow of uniformity, the be translucent liquid of uniformity of outward appearance, has cider and the intrinsic fragrance of giant salamander enzymolysis liquid and flavour body.
The preparation method of above-mentioned Passion Fruit Juice is:
(1) select more than 95% well-done passion fruit, remove after pericarp, obtain passion fruit pulp, then add appropriate water and in temperature be under the condition of 80-95 DEG C and carry out infusion 30 minutes, then filter, then at vacuum 0.08Mpa, temperature is vacuum impregnation 25min under the condition of 60 DEG C, under normal pressure, flood 2-4 hour again, obtain passion fruit Normal juice;
(2) in the weight portion of passion fruit pulp, rock sugar 20-40 part, HFCS 5-30 part, brown sugar 0.5-1.5 part and water 30-150 part are put in pot and is heated to 70-90 DEG C, be stirred to completely and melt;
(3) solution obtaining in the passion fruit Normal juice of gained in step (1) and step (2) is mixed, obtain Passion Fruit Juice.In weight portion, 150 parts, water in 80 parts of passion fruit pulp, step (1), rock sugar 20-40 part, HFCS 5-30 part, brown sugar 0.5-1.5 part, the middle water 30-150 part of step (2).
The preparation method of described ginkgo extract is:
(3) get fresh ginkgo leaf, then make its air drying 3-5 days under field conditions (factors), then it is added water in iron pan carry out infusion, the temperature of infusion is 80-95 DEG C, and the time is 20-50 minute, then filters; Obtain the extract of ginkgo leaf; In weight portion, fresh ginkgo leaf is 50～100 parts; The addition of water is 200-300 part.
(4) get commercially available ginkgo fruit, make, after its fragmentation, fruit was pulverized to 200 mesh sieves, obtain ginkgo pulp; Then together with the shell of ginkgo fruit being added water with the leaf slag of the ginkgo leaf obtaining in step (1), carry out infusion, the temperature of infusion is 90-95 DEG C, and the time is 3-5h, then filters, and obtains filtrate; The weight portion of ginkgo fruit is 20-40 part, and the weight portion of water is 120-160 part.(3) filtrate that obtains in the filtrate obtaining in step (1) and step (2) is mixed, then add the ginkgo fruit of pulverizing, and white granulated sugar mixes, obtain ginkgo extract.In weight portion, the weight portion of white granulated sugar is 30-60 part.
Embodiment 5:
The preparation of giant salamander polypeptide is with embodiment 1, the preparation of giant salamander polypeptide beverage: (1) is by the saccharogenesis water filtration removal of impurities that is dissolved in water of raw material sugar; (2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid, ginkgo extract and Passion Fruit Juice are incorporated to stirring and dissolving in appropriate water, add again syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide beverage is 0.30%; (3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid; (4) product liquid being carried out to filling and sterilization forms.
Wherein: described tartaric acid is made up of taking mass ratio as 3:1 citric acid and malic acid.Described fruit juice is Passion Fruit Juice; Each constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthans 0.15%, potassium sorbate 0.025%, citric acid 0.3%, malic acid 0.1%, Passion Fruit Juice 10%, ginkgo extract 4%, dandelion extract 0.8%, all the other are giant salamander polypeptide liquid, in preparation, control content of peptides in giant salamander polypeptide beverage by adding water, water can come from giant salamander polypeptide liquid or raw material components dissolves water.
The preparation method of Passion Fruit Juice and ginkgo extract is with embodiment 4.
Embodiment 6:
The preparation of giant salamander polypeptide is with embodiment 1, the preparation of giant salamander polypeptide beverage: (1) is by the saccharogenesis water filtration removal of impurities that is dissolved in water of raw material sugar; (2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid, ginkgo extract and Passion Fruit Juice are incorporated to stirring and dissolving in appropriate water, add again syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide beverage is 0.35%; (3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid; (4) product liquid being carried out to filling and sterilization forms.
Wherein: described tartaric acid is made up of taking mass ratio as 3:1 citric acid and malic acid.Described fruit juice is Passion Fruit Juice; Each constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthans 0.15%, potassium sorbate 0.025%, citric acid 0.3%, malic acid 0.1%, Passion Fruit Juice 10%, ginkgo extract 5%, dandelion extract 1%, all the other are giant salamander polypeptide liquid, in preparation, control content of peptides in giant salamander polypeptide beverage by adding water, water can come from giant salamander polypeptide liquid or raw material components dissolves water.
The preparation method of Passion Fruit Juice and ginkgo extract is with embodiment 4.
The giant salamander polypeptide beverage obtaining in embodiment 1-embodiment 6 is analyzed, adopt the giant salamander polypeptide beverage color and luster of system of the present invention to be the golden yellow or light yellow of uniformity, the be translucent liquid of uniformity of outward appearance, there is Passion Fruit Juice and the intrinsic fragrance of giant salamander enzymolysis liquid and flavour body, more owing to having added ginkgo extract and Passion Fruit Juice; And appropriate dandelion extract, make giant salamander polypeptide beverage nutritive value of the present invention abundant, can utilize each component of formula itself to overcome the special foreign odor of giant salamander polypeptide, there is mouthfeel and raciness, be of high nutritive value, green safety, facilitate the daily high-quality polypeptide to giant salamander to supplement; Adopt the giant salamander polypeptide of described method system, can effectively avoid giant salamander horny layer of epidermis on the impact of enzymolysis process and enzymolysis product, enzymatic hydrolysis condition gentleness, can carry out sufficient enzymolysis to Megalobatrachus japonicus daoidianuas(Blanchard), and enzymolysis process is easy to control, enzymolysis product safety and be beneficial to absorption of human body.
Comparative example 1:
Taking embodiment 4 as example, on the basis of embodiment 4, do not add ginkgo extract, all the other preparation method's steps are consistent with embodiment 2.
Comparative example 2:
Taking embodiment 4 as example, on the basis of embodiment 4, do not add giant salamander polypeptide raw material, all the other preparation method's steps are consistent with embodiment 2.
By embodiment 1 to embodiment 6, and the drink obtaining in comparative example 1 and comparative example 2 protects liver measure of merit
160 mouse, male and female half and half, are divided into 8 groups at random, 20 every group, comprise 20 every group of embodiment 1-6, every group also 20 of comparative example 1-2.The first fasting 12h of each group mouse, the product then embodiment 1-6 and comparative example 1-2 all being drawn preparation carries out the gastric infusion of each 20ml, and 1h fills with 2 times, spends Red Star strong, colourless liquor distilled from sorghum white wine 0.16ml/10g then after 1h, to the each group of equal gavage 56 of mouse.After 12h, weigh, gather liver specimens, do coherent detection.ALT, AST press kit description colorimetric method for determining, and ALT and the AST concentration in cell is to detect the important indicator of hepar damnification.
*P<0.05；
Compare proof with comparative example 1, embodiment 1, embodiment 2 and embodiment 3 can effectively reduce the rising of ALT, AST, represent that its ALT, AST that can contain ethanol and cause raises, and has protective effect to drunk mouse liver injury; The effect of embodiment 4, embodiment 5 or embodiment 6 is significantly better than embodiment 1, embodiment 2 and embodiment 4 and comparative example 1 and comparative example 2, represent to have added the giant salamander polypeptide beverage of ginkgo extract, its effect of protecting liver is more remarkable, and the interpolation of giant salamander polypeptide and the interpolation of ginkgo extract have better effect.
Compare proof with comparative example 2, giant salamander polypeptide has certain liver protection effect, but DeGrain only has with ginkgo extract compoundly, could be more effective play the effect of protecting liver.
Owing to containing gingko alcohol, bilobanone and stigmasterol in ginkgo extract.Ginkgo is extracted and contains 17 kinds of human bodies " essential amino acid ", also contains the necessary trace element of human body; And lysine content in giant salamander polypeptide is high, with giant salamander polypeptide be development of raw materials go out to meet lysine preparations also more, but it is inadequate only containing lysine, also should carry out compound with other material that contains other kind of amino acid or human needs.Preparation meets the nutrition fortifier of human nutrition requirement.Not only unique flavor of white fragrant fruit juice also in this raw material, and healthcare, help digest, have eliminate tired, refresh oneself and sober up, anti-inflammatory nti-freckle, beautifying face and moistering lotion, prevent the effects such as artery sclerosis, through overtesting certification, also can carry out well composite with giant salamander polypeptide.
The present invention is not limited to aforesaid detailed description of the invention.The present invention expands to any new feature or any new combination disclosing in this manual, and the arbitrary new method disclosing or step or any new combination of process.
Claims (10)
1. a giant salamander polypeptide beverage, it is characterized in that: comprise giant salamander polypeptide liquid, sugar, beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid and fruit juice, each constituent mass mark is sugared 5-15%, beta-schardinger dextrin-0.05-0.15%, xanthans 0.05-0.15%, potassium sorbate 0.01-0.025%, tartaric acid 0.15-0.4%, fruit juice 3-10%, and all the other are giant salamander polypeptide liquid.
2. giant salamander polypeptide beverage as claimed in claim 1, is characterized in that: described tartaric acid is made up of taking mass ratio as 3-6:1 citric acid and malic acid, and described fruit juice is cider.
3. giant salamander polypeptide beverage as claimed in claim 2, it is characterized in that: described each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthans 0.1%, potassium sorbate 0.02%, citric acid 0.25%, malic acid 0.05%, cider 5%, all the other are giant salamander polypeptide liquid.
4. giant salamander polypeptide beverage as claimed in claim 2, it is characterized in that: described each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthans 0.1%, potassium sorbate 0.02%, citric acid 0.25%, malic acid 0.05%, white fragrant fruit juice 5%, ginkgo extract 3%, dandelion extract 0.6%, all the other are giant salamander polypeptide liquid.
5. giant salamander polypeptide beverage as claimed in claim 1 or 2 or 3 or 4, is characterized in that: in described giant salamander polypeptide beverage, content of peptides is not less than 2%, and the molecular weight of described giant salamander polypeptide liquid is not more than 20000Da.
6. the preparation method of giant salamander polypeptide beverage as claimed in claim 1, is characterized in that: comprise following steps:
(1) raw material sugar is dissolved in water saccharogenesis water filtration removal of impurities;
(2) beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid are incorporated to stirring and dissolving in appropriate water, then add syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, control content of peptides in giant salamander polypeptide beverage and be not less than 0.02%;
(3) stoste after filtering is evenly mixed by colloid mill, obtain uniform and stable product liquid;
(4) product liquid being carried out to filling and sterilization forms.
7. the preparation method of giant salamander polypeptide beverage as claimed in claim 6, is characterized in that: described giant salamander polypeptide liquid makes as follows:
(a) pretreatment: giant salamander is cleaned and stripping and slicing;
(b) infusion: add water to gained giant salamander cube meat, infusion is to giant salamander cube meat organization softening;
(c) enzymolysis: after feed liquid after infusion is cooling, moisturizing regulates solid-liquid ratio, add compound protease to carry out enzymolysis, described compound protease comprises neutral proteinase, papain and flavor protease composition again, and described flavor protease is made up of aminopeptidase and carboxypeptidase;
(d) enzyme that goes out: after enzymolysis completes, enzymolysis liquid is boiled to the enzyme that goes out;
(e) filter: the feed liquid of going out after enzyme is carried out to multistage filtering, the pure polypeptide products of system.
8. the preparation method of giant salamander polypeptide as claimed in claim 7, is characterized in that: in described step (c), described neutral protein enzyme dosage is the 0.5-1.5% of giant salamander cube meat quality, and papain consumption is the 0.6-2.0% of giant salamander cube meat quality; Described flavor protease is made up of with mass ratio 3-5:1 aminopeptidase and carboxypeptidase, and flavor protease consumption is the 0.3-1.0% of giant salamander cube meat quality.
9. the preparation method of giant salamander polypeptide as claimed in claim 8, it is characterized in that: in described step (c), described neutral protein enzyme dosage is 1.2% of giant salamander cube meat quality, papain consumption is 1.6% of giant salamander cube meat quality, described flavor protease is made up of with mass ratio 4:1 aminopeptidase and carboxypeptidase, and flavor protease consumption is 0.8% of giant salamander cube meat quality; In described step in (e), adopt three grades of step apertures to filter, 30-50 μ m, 5 μ m, the 0.45 μ m of being followed successively by different levels, finally adopting interception is that the milipore filter of 20000Da carries out ultrafiltration.
10. the preparation method of giant salamander polypeptide beverage as claimed in claim 6, it is characterized in that: in step (2), beta-schardinger dextrin-, xanthans, potassium sorbate, tartaric acid, ginkgo extract and dandelion extract are incorporated to stirring and dissolving in appropriate water, add again syrup, giant salamander polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, control content of peptides in giant salamander polypeptide beverage and be not less than 0.02%.
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Cited By (6)
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| CN106107397A (en) * | 2016-06-27 | 2016-11-16 | 张博 | A kind of Andrias davidianus Blanchard protein peptide powder solid beverage formula and preparation method thereof |
| CN108042786A (en) * | 2017-12-25 | 2018-05-18 | 高安友 | A kind of compound oral drink of giant salamander polypeptide powder for being used to increase bone density |
| CN109845929A (en) * | 2019-01-23 | 2019-06-07 | 辽宁科技大学 | A kind of mung bean antioxidation polypeptide beverage and preparation method thereof |
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| CN111034997A (en) * | 2019-12-23 | 2020-04-21 | 张家界（中国）金驰大鲵生物科技有限公司 | Giant salamander active peptide composition with anti-aging, blood pressure regulating and anti-anoxia effects |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000308483A (en) * | 1999-04-28 | 2000-11-07 | Yukinobu Toe | Use of microorganism |
| CN102090684A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Marine collagen peptide beverage and preparation method thereof |
| CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
| CN102406205A (en) * | 2011-12-05 | 2012-04-11 | 张家界（中国）金驰大鲵生物科技有限公司 | Oyster polysaccharide fruit juice beverage |
| CN103493969A (en) * | 2013-07-29 | 2014-01-08 | 四川龙王洞生态农业开发有限公司 | Method for producing giant salamander polypeptide powder |
| CN103621869A (en) * | 2013-11-15 | 2014-03-12 | 贵阳学院 | Giant salamander oligosaccharide peptide health-care product |
-
2014
- 2014-06-16 CN CN201410264789.1A patent/CN104041881A/en active Pending
- 2014-08-15 CN CN201410404611.2A patent/CN104172353B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000308483A (en) * | 1999-04-28 | 2000-11-07 | Yukinobu Toe | Use of microorganism |
| CN102090684A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Marine collagen peptide beverage and preparation method thereof |
| CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
| CN102406205A (en) * | 2011-12-05 | 2012-04-11 | 张家界（中国）金驰大鲵生物科技有限公司 | Oyster polysaccharide fruit juice beverage |
| CN103493969A (en) * | 2013-07-29 | 2014-01-08 | 四川龙王洞生态农业开发有限公司 | Method for producing giant salamander polypeptide powder |
| CN103621869A (en) * | 2013-11-15 | 2014-03-12 | 贵阳学院 | Giant salamander oligosaccharide peptide health-care product |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106107397A (en) * | 2016-06-27 | 2016-11-16 | 张博 | A kind of Andrias davidianus Blanchard protein peptide powder solid beverage formula and preparation method thereof |
| CN108042786A (en) * | 2017-12-25 | 2018-05-18 | 高安友 | A kind of compound oral drink of giant salamander polypeptide powder for being used to increase bone density |
| CN109845929A (en) * | 2019-01-23 | 2019-06-07 | 辽宁科技大学 | A kind of mung bean antioxidation polypeptide beverage and preparation method thereof |
| CN110432417A (en) * | 2019-09-03 | 2019-11-12 | 湖北世厚耀榜纪田农业发展有限公司 | A kind of giant salamander protein peptide drinks and preparation method thereof with liver-protecting function |
| CN111034997A (en) * | 2019-12-23 | 2020-04-21 | 张家界（中国）金驰大鲵生物科技有限公司 | Giant salamander active peptide composition with anti-aging, blood pressure regulating and anti-anoxia effects |
| CN112715867A (en) * | 2020-12-28 | 2021-04-30 | 山东蓝奥生物技术有限公司 | Preparation method of flower glue and flower glue |
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| CN104172353B (en) | 2017-01-11 |
| CN104041881A (en) | 2014-09-17 |
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