CN107326056A - The preparation method of giant salamander polypeptide powder - Google Patents
The preparation method of giant salamander polypeptide powder Download PDFInfo
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- CN107326056A CN107326056A CN201710604123.XA CN201710604123A CN107326056A CN 107326056 A CN107326056 A CN 107326056A CN 201710604123 A CN201710604123 A CN 201710604123A CN 107326056 A CN107326056 A CN 107326056A
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- giant salamander
- cube meat
- preparation
- enzymolysis
- polypeptide powder
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The invention discloses a kind of preparation method of giant salamander polypeptide powder, process in accordance with the following steps：1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughters gall-bladder and adipose tissue；2) giant salamander is cleaned up, and is cut into cube meat taking-up and drained the water, it is standby；3) with sodium bicarbonate solution to giant salamander cube meat ungrease treatment, 4) defishying is carried out to giant salamander cube meat with jasmine tea, calcium chloride composite solution；5) cook:It will be added water infusion, softening tissue by the giant salamander cube meat of defishying.6) digest:The compound protease of elastoser to be added and papain composition is digested；7) inactivate:Enzymolysis liquid is boiled into inactivation after the completion of enzymolysis；8) filter:Feed liquid after inactivation is subjected to multistage filtering, ultrafiltration, pure polypeptide filtrate is made.The preparation method of the giant salamander polypeptide powder of the present invention is simply efficient, can avoid giant salamander cuticula to the adverse effect caused by polypeptide yield and quality, improves enzymolysis quality.
Description
Technical field
The present invention relates to a kind of preparation method of giant salamander polypeptide powder, belong to biologically active peptide field.
Background technology
Giant salamander is commonly called as the giant salamander, belongs to Amphibia Caudata Cryptobranchidae, is species more original in existing amphibian.
It is that China is with a long history, there is the evolutionary history up to 3.5 hundred million years, the living fossil that can be compared favourably with giant panda, is that China is traditional
Medicinal animal, early in the medical treasured book of Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》Described in, the medicinal folk prescription of folklore is also a lot of, through the modern times
Analysis on Nutritious Ingredient of Fattening result of study shows：Its is nutritious, containing 18 kinds of amino acid, and composition composition is comprehensive, is that human nutrition is needed
The high-quality protein source asked, is the treasure of " asking it in the four seas ".With the success of artificial breeding, giant salamander is in the important meaning academicly studied
Justice is further elevated, and people, which are constantly recognized the edibility and effect of giant salamander, to deepen continuously.Unfortunately, claim
The deep processed product that giant salamander must be gone up is very few.
The B of CN 103493969 disclose a kind of method for producing giant salamander polypeptide powder, by simple pretreatment, using food
Carried and just directly being digested after degreasing with oil immersion.
The A of CN 104068204 disclose a kind of preparation method of giant salamander polypeptide powder, after giant salamander stripping and slicing, infusion, then just directly
Enzymolysis.
The A of CN 104206641 also disclose that a kind of giant salamander polypeptide powder and preparation method thereof, also by rejecting
Directly digested after the adipose tissue and gall-bladder of giant salamander.That is, its preparation of the research of current giant salamander polypeptide powder is limited to digest bar
Piece optimization.It has ignored and processing is impacted due to the black cuticula that the crust of giant salamander has so that giant salamander was digesting
In journey not thoroughly, enzymolysis process and product are impacted, so as to influence the quality of product.
The content of the invention
In order to solve the above-mentioned technical problem, it is an object of the invention to provide a kind of convenience and the system of efficient giant salamander polypeptide powder
Preparation Method, it is to avoid influence of the cuticula to enzymolysis, improves product quality.
To achieve these goals, technical scheme is as follows：A kind of preparation method of giant salamander polypeptide powder, its feature exists
In processing in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughtered, and remove gall-bladder and adipose tissue；
2) pre-process：Giant salamander is cleaned up, and is cut into cube meat taking-up and is drained the water, it is standby；
3) degreasing：Ungrease treatment is carried out to giant salamander cube meat with sodium bicarbonate solution, skimming temp is 5-10 DEG C；
4) deodorant：Defishying is carried out to giant salamander cube meat with jasmine tea, calcium chloride composite solution, deodorant temperature is 5-10 DEG C；
5) cook:It will be added water infusion by the giant salamander cube meat of defishying, the temperature control of infusion process is at 95 DEG C -100
DEG C, the infusion time is defined by giant salamander cube meat organization softening；
6) digest:After after the feed liquid cooling after infusion, moisturizing regulation solid-liquid ratio, stirring adds elastoser and wood
The compound protease of melon protease composition is digested, 40-50 DEG C of hydrolysis temperature, and pH controls are in 5.5-6.5, enzymolysis during enzymolysis
Between 5-6h；
7) inactivate:Enzymolysis liquid is boiled into inactivation after the completion of enzymolysis；
8) filter:Feed liquid after inactivation is subjected to multistage filtering, ultrafiltration, pure polypeptide filtrate is made.
Using such scheme, giant salamander cube meat first using sodium bicarbonate solution degreasing and then uses jasmine tea, calcium chloride deodorant,
In this process, fat can not only be removed, removes fishy smell, moreover it is possible to the black cuticula of giant salamander epidermis is removed, black corners are reduced
Matter layer is impacted to enzymolysis, improves enzymolysis product quality.Unlike the prior art, the present invention uses elastoser and pawpaw
The complex enzyme of protease composition is digested, and improves enzymolysis quality, the partially white (existing process of obtained polypeptide color light yellow
Obtained polypeptide is yellow), quality is good, is conducive to absorbing.By polypeptide filtrate using being spray-dried, obtained polypeptide powder is received
Rate is higher than 30%.
In such scheme：In step (2), giant salamander is cut into 1-3cm giant salamander cube meat.Be conducive to degreasing, deodorant and raising
Enzymolysis efficiency.
In such scheme：In step (3), the mass concentration of sodium bicarbonate solution is 0.1-2%, sodium bicarbonate solution with it is big
The mass ratio of salamander cube meat is 5-7：1, degreasing time 0.5-2h.Degreasing effect is good.
In such scheme：In composite solution：The mass fraction of jasmine tea is 0.1-0.5%, and the mass fraction of calcium chloride is
0.1%-0.2%, composite solution is 4-6 with giant salamander cube meat mass ratio:1, deodorant time 1-3h.Deodorant destratum corneum effect is good.
In such scheme：Step 5) in, during cooking, the weight ratio of giant salamander cube meat and water is 1:1, brew time 30-
45min.Be conducive to organization softening.
In such scheme：Step 6) in, moisturizing regulation solid-liquid ratio 1:2, the elastoser is 100,000 U/g, and consumption is
The 0.3-0.7% of giant salamander cube meat quality, papain is 100,000 U/g, and consumption is the 0.8-1.2% of giant salamander cube meat quality.Carry
Height enzymolysis quality, obtained peptide masses are good, are conducive to absorption of human body.
In such scheme：Step 7) in, inactivation temperature is 90 DEG C -100 DEG C, and inactivation time is 10-30min.
In such scheme：Step 8) in, it is at different levels to be followed successively by 40 μm, 2 μm, 0.45 μm using three-level step bore filter, most
Interception is used to carry out ultrafiltration for 20000Da milipore filter afterwards.
Beneficial effect：The preparation method of the giant salamander polypeptide powder of the present invention is simply efficient, can avoid giant salamander cuticula to polypeptide
Adverse effect caused by yield and quality, while digested using the complex enzyme of elastoser and papain composition,
Enzymolysis quality is improved, obtained polypeptide, nutrient loss is small, beneficial to the absorption of human body, available for medical material, medicine, work(
Energy food etc., is adapted to industrialized production.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1
A kind of preparation method of giant salamander polypeptide powder, is processed in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughters the position such as gall-bladder and giant salamander peritonaeum, afterbody, four limbs
Adipose tissue, remainder be used for prepare polypeptide.
2) pre-process：Giant salamander is cleaned up, and is cut into 1-3cm giant salamander cube meat taking-up and is drained the water, it is standby.
3) degreasing：With sodium bicarbonate solution to giant salamander cube meat ungrease treatment, the mass concentration of sodium bicarbonate solution is
0.1%, the mass ratio of sodium bicarbonate solution and giant salamander cube meat is 7：1, degreasing time 2h, skimming temp are 5-10 DEG C.
4) deodorant：Giant salamander cube meat is carried out in defishying, composite solution with jasmine tea, calcium chloride composite solution：Jasmine tea
Mass fraction is 0.1%, and the mass fraction of calcium chloride is 0.1%%, and composite solution is 6 with giant salamander cube meat mass ratio:1, deodorant
Time 3h, deodorant temperature is 5-10 DEG C.
5) cook:It will be added water infusion by the giant salamander cube meat of defishying, the weight ratio of giant salamander cube meat and water is 1:1, endure
The temperature control of process is boiled at 95 DEG C -100 DEG C, brew time 30-45min is defined by giant salamander cube meat organization softening.
6) digest:After after the feed liquid cooling after infusion, moisturizing adjusts solid-liquid ratio 1:2, stirring, add elastoser and
The compound protease of papain composition is digested, and elastoser is 100,000 U/g, and consumption is giant salamander cube meat quality
0.3%, papain is 100,000 U/g, and consumption is the 1.2% of giant salamander cube meat quality.40-50 DEG C of hydrolysis temperature, pH during enzymolysis
Control is in 5.5-6.5, enzymolysis time 5h.
7) inactivate:Enzymolysis liquid is boiled into inactivation after the completion of enzymolysis, inactivation temperature is 90 DEG C -100 DEG C, and inactivation time is 10-
30min。
8) filter:Feed liquid after inactivation is subjected to multistage filtering, ultrafiltration.Specifically：Using three-level step bore filter, respectively
Level is followed successively by 40 μm, 2 μm, 0.45 μm, finally use the milipore filter progress ultrafiltration that interception is 20000Da, obtained pure many
Peptide filtrate.Filtrate can obtain polypeptide powder by spray drying.
Embodiment 2
A kind of preparation method of giant salamander polypeptide powder, is processed in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughters the position such as gall-bladder and giant salamander peritonaeum, afterbody, four limbs
Adipose tissue, remainder be used for prepare polypeptide.
2) pre-process：Giant salamander is cleaned up, and is cut into 1-3cm giant salamander cube meat taking-up and is drained the water, it is standby.
3) degreasing：With sodium bicarbonate solution to giant salamander cube meat ungrease treatment, the mass concentration of sodium bicarbonate solution is 2%,
The mass ratio of sodium bicarbonate solution and giant salamander cube meat is 5：1, degreasing time 0.5h, skimming temp are 5-10 DEG C.
4) deodorant：Giant salamander cube meat is carried out in defishying, composite solution with jasmine tea, calcium chloride composite solution：Jasmine tea
Mass fraction is 0.5%, and the mass fraction of calcium chloride is 0.2%, and composite solution is 4 with giant salamander cube meat mass ratio:1, during deodorant
Between 1h, deodorant temperature be 5-10 DEG C.
5) cook:It will be added water infusion by the giant salamander cube meat of defishying, the weight ratio of giant salamander cube meat and water is 1:1, endure
The temperature control of process is boiled at 95 DEG C -100 DEG C, brew time 30-45min is defined by giant salamander cube meat organization softening.
6) digest:After after the feed liquid cooling after infusion, moisturizing adjusts solid-liquid ratio 1:2, stirring, add elastoser and
The compound protease of papain composition is digested, and elastoser is 100,000 U/g, and consumption is giant salamander cube meat quality
0.7%, papain is 100,000 U/g, and consumption is the 0.8% of giant salamander cube meat quality.40-50 DEG C of hydrolysis temperature, pH during enzymolysis
Control is in 5.5-6.5, enzymolysis time 6h.
7) inactivate:Enzymolysis liquid is boiled into inactivation after the completion of enzymolysis, inactivation temperature is 90 DEG C -100 DEG C, and inactivation time is 10-
30min。
8) filter:Feed liquid after inactivation is subjected to multistage filtering, ultrafiltration.Specifically：Using three-level step bore filter, respectively
Level is followed successively by 40 μm, 2 μm, 0.45 μm, finally use the milipore filter progress ultrafiltration that interception is 20000Da, obtained pure many
Peptide filtrate.Filtrate can obtain polypeptide powder by spray drying.
Embodiment 3
A kind of preparation method of giant salamander polypeptide powder, is processed in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughters the position such as gall-bladder and giant salamander peritonaeum, afterbody, four limbs
Adipose tissue, remainder be used for prepare polypeptide.
2) pre-process：Giant salamander is cleaned up, and is cut into 1-3cm giant salamander cube meat taking-up and is drained the water, it is standby.
3) degreasing：With sodium bicarbonate solution to giant salamander cube meat ungrease treatment, the mass concentration of sodium bicarbonate solution is 1%,
The mass ratio of sodium bicarbonate solution and giant salamander cube meat is 6：1, degreasing time 1h, skimming temp are 5-10 DEG C.4) deodorant：With flower
Tea, calcium chloride composite solution are carried out in defishying, composite solution to giant salamander cube meat：The mass fraction of jasmine tea is 0.2%, chlorination
The mass fraction of calcium is 0.2%, and composite solution is 5 with giant salamander cube meat mass ratio:1, deodorant time 2h, deodorant temperature are 5-10
℃。
5) cook:It will be added water infusion by the giant salamander cube meat of defishying, the weight ratio of giant salamander cube meat and water is 1:1, endure
The temperature control of process is boiled at 95 DEG C -100 DEG C, brew time 30-45min is defined by giant salamander cube meat organization softening.
6) digest:After after the feed liquid cooling after infusion, moisturizing adjusts solid-liquid ratio 1:2, stirring, add elastoser and
The compound protease of papain composition is digested, and elastoser is 100,000 U/g, and consumption is giant salamander cube meat quality
0.5%, papain is 100,000 U/g, and consumption is the 1% of giant salamander cube meat quality.40-50 DEG C of hydrolysis temperature, pH is controlled during enzymolysis
System is in 5.5-6.5, enzymolysis time 6h.
7) inactivate:Enzymolysis liquid is boiled into inactivation after the completion of enzymolysis, inactivation temperature is 90 DEG C -100 DEG C, and inactivation time is 10-
30min。
8) filter:Feed liquid after inactivation is subjected to multistage filtering, ultrafiltration.Specifically：Using three-level step bore filter, respectively
Level is followed successively by 40 μm, 2 μm, 0.45 μm, finally use the milipore filter progress ultrafiltration that interception is 20000Da, obtained pure many
Peptide filtrate.Filtrate can obtain polypeptide powder by spray drying.
The present invention is not limited to above-mentioned specific embodiment, it will be appreciated that one of ordinary skill in the art is without creative
Work just can make many modifications and variations according to the design of the present invention.In a word, all technical staff in the art are according to this
The design of invention passes through the available technical side of logical analysis, reasoning, or a limited experiment on the basis of existing technology
Case, all should be in the protection domain being defined in the patent claims.
Claims (8)
1. a kind of preparation method of giant salamander polypeptide powder, it is characterised in that process in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughtered, and remove gall-bladder and adipose tissue；
2) pre-process：Giant salamander is cleaned up, and is cut into cube meat taking-up and is drained the water, it is standby；
3) degreasing：Ungrease treatment is carried out to giant salamander cube meat with sodium bicarbonate solution, skimming temp is 5-10 DEG C；
4) deodorant：Defishying is carried out to giant salamander cube meat with jasmine tea, calcium chloride composite solution, deodorant temperature is 5-10 DEG C；
5) cook:It will be added water infusion by the giant salamander cube meat of defishying, the temperature control of infusion process is endured at 95 DEG C -100 DEG C
Boil the time is defined by giant salamander cube meat organization softening；
6) digest:After after the feed liquid cooling after infusion, moisturizing regulation solid-liquid ratio, stirring adds elastoser and pawpaw egg
The compound protease of white enzyme composition is digested, 40-50 DEG C of hydrolysis temperature, and pH controls are in 5.5-6.5, enzymolysis time 5- during enzymolysis
6h；
7) inactivate:Enzymolysis liquid is boiled into inactivation after the completion of enzymolysis；
8) filter:Feed liquid after inactivation is subjected to multistage filtering, ultrafiltration, pure polypeptide filtrate is made.
2. the preparation method of giant salamander polypeptide powder according to claim 1, it is characterised in that：In step (2), giant salamander is cut into 1-
3cm giant salamander cube meat.
3. the preparation method of giant salamander polypeptide powder according to claim 1, it is characterised in that：In step (3), sodium bicarbonate solution
Mass concentration is 0.1-2%, and the mass ratio of sodium bicarbonate solution and giant salamander cube meat is 5-7：1, degreasing time 0.5-2h.
4. the preparation method of giant salamander polypeptide powder according to claim 3, it is characterised in that：In composite solution：The quality of jasmine tea point
Number is 0.1-0.5%, and the mass fraction of calcium chloride is 0.1%-0.2%, composite solution is 4-6 with giant salamander cube meat mass ratio:1,
Deodorant time 1-3h.
5. according to the preparation method of any one of the claim 1-4 giant salamander polypeptide powders, it is characterised in that：Step 5) in, during cooking,
The weight ratio of giant salamander cube meat and water is 1:1, brew time 30-45min.
6. the preparation method of giant salamander polypeptide powder according to claim 5, it is characterised in that：Step 6) in, moisturizing regulation solid-liquid ratio
1:2, the elastoser is 100,000 U/g, and consumption is the 0.3-0.7% of giant salamander cube meat quality, and papain is 100,000 U/
G, consumption is the 0.8-1.2% of giant salamander cube meat quality.
7. the preparation method of giant salamander polypeptide powder according to claim 6, it is characterised in that：Step 7) in, inactivation temperature be 90 DEG C-
100 DEG C, inactivation time is 10-30min.
8. the preparation method of giant salamander polypeptide powder according to claim 7, it is characterised in that：Step 8) in, using three-level step hole
Footpath is filtered, at different levels to be followed successively by 40 μm, 2 μm, 0.45 μm, finally uses interception to carry out ultrafiltration for 20000Da milipore filter.
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| CN104068204A (en) * | 2014-06-16 | 2014-10-01 | 四川溪源水产养殖有限公司 | Preparation method of giant salamander polypeptides |
| CN104982970A (en) * | 2015-08-06 | 2015-10-21 | 重庆馗丰食品有限公司 | Making method and product of pure giant salamander meat meal |
| CN105018555A (en) * | 2015-08-06 | 2015-11-04 | 重庆馗丰食品有限公司 | Preparation method of giant salamander skin collagen peptide |
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2017
- 2017-07-21 CN CN201710604123.XA patent/CN107326056A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008087442A1 (en) * | 2007-01-19 | 2008-07-24 | Evocell Limited | Biological materials and uses thereof |
| CN104041881A (en) * | 2014-06-16 | 2014-09-17 | 四川溪源水产养殖有限公司 | Giant salamander polypeptide beverage and preparation method thereof |
| CN104068204A (en) * | 2014-06-16 | 2014-10-01 | 四川溪源水产养殖有限公司 | Preparation method of giant salamander polypeptides |
| CN104982970A (en) * | 2015-08-06 | 2015-10-21 | 重庆馗丰食品有限公司 | Making method and product of pure giant salamander meat meal |
| CN105018555A (en) * | 2015-08-06 | 2015-11-04 | 重庆馗丰食品有限公司 | Preparation method of giant salamander skin collagen peptide |
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| Title |
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| WENTAO GUO ET AL.: "Major Biological Activities of the Skin Secretion of the Chinese Giant Salamander, Andrias davidianus", 《Z.NATURFORSCH.》 * |
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Application publication date: 20171107 |