CN108308620A - The method that edible protein enzyme digests animal organ and bone prepares functional peptide food - Google Patents
The method that edible protein enzyme digests animal organ and bone prepares functional peptide food Download PDFInfo
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- CN108308620A CN108308620A CN201810254445.0A CN201810254445A CN108308620A CN 108308620 A CN108308620 A CN 108308620A CN 201810254445 A CN201810254445 A CN 201810254445A CN 108308620 A CN108308620 A CN 108308620A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/21—Removal of unwanted matter, e.g. deodorisation or detoxification by heating without chemical treatment, e.g. steam treatment, cooking
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses the method that a kind of edible protein enzyme enzymolysis animal organ and bone prepare functional peptide food, step includes stock, and fermentation enzymolysis enzyme deactivation, filters, boils inactivation, steam distillation deodorant and seasoning, desensitization；The present invention uses edible protein enzyme for catalyst, hydrolyze the organ and bone of edible animal, it releases biologically active " functional peptide ", as food to enhance the function of human body corresponding organ, to which the advanced tonic for making those be now only booming income crowd enjoyment is all used as rich or poor, the very high popular food of suitable for all ages, nutritive value is promoted fitness-for-all and is contributed to improve whole people's nutrition.
Description
Technical field
It is the present invention relates to a kind of food production technology, more particularly to a kind of to utilize edible protein enzyme enzymolysis animal organ and bone
The method that bone prepares " functional peptide " food belongs to Education of Food Biochemistry technical field.
Background technology
Modern biochemistry is the study found that the specific function of animal organ derives from the " functional protein for constituting the organ
Matter ", and the function of these specific proteins derives from and constitutes their " functional peptide ", and pass through the water of albumen enzymatic
The activity " functional peptide " that these can be present in native organism by solution reaction releases, as food, to promote people
The health service of class.Certain " functional peptides " also have immunological regulation, hormone control, enzyme inhibition, antibacterial, disease-resistant as food
Poison is stabilized the effect of hypertension or even anticancer, thus in bioengineering field, has biology with the technological development of protease hydrolyzed
Active " functional peptide " is quickly grown.The functional characteristic that protein food is improved by enzymolysis modified technology has become close
The emphasis that food science and technology is studied over year.
The discovery and application of " functional peptide " are a biochemical much progresses.But the research of " functional peptide " now
It is scarcely out of swaddling-clothes with application.This is mainly manifested in following two aspects：The research and production of current 1. " functional peptide ", base
It is confined to make the scope of raw material using cheap phytoprotein in sheet.Although in China, someone has produced with health care
" holoprotein peptide " supply market of function simultaneously achieves certain economic benefit and social benefit, but using animal protein the bottom of as
Material develops the work of " functional peptide ", is also rarely reported.The research of current 2. " functional peptide " also lacks ripe theoretical direction,
Specific target and direction are technically especially also lacked how finding and manufacture new " functional peptide ", are hovered and are rested on examination
The stage of fumbling for the property visited.Thus, till now, there are no develop any type to have special health to human body certain organs
The foodstuff " functional peptide " of effect.
Invention content
Technical problem to be solved by the present invention lies in provide a kind of edible protein enzyme enzymolysis animal organ and be prepared by bone
The method of functional peptide food makes the protein digestion of animal organ and bone release " functional peptide " using edible protein enzyme
As health food.
In order to solve the above technical problems, the present invention uses technical solution below：
The method that edible protein enzyme digests animal organ and bone prepares functional peptide food, includes the following steps,
（1）Stock：When using animal organ as enzymolysis bottom material, stock up according to the following steps：A. selected animal organ is cleaned
After be cut into small pieces, be put into pot, tap water be added, is filtered after boiling 3-10 minute, abandons soup stock, to remove in animal meat piece spy
Some fishy smell；B. the pure water of 2-6 times of animal organ's weight is added, boils 1-4h, the tight junctions of meat is destroyed, in favor of enzyme
Solution reaction is smoothed out；C. meat piece is transferred to together with soup stock in wall-breaking machine, starts wall-breaking machine, meat piece is stirred into meat slurry, waits for meat
After slurry is cooled to the hydrolysis temperature selected needed for protease or less, it is transferred in enzymatic hydrolysis and fermentation tank, suitable table egg is added
White enzyme, stirring make edible protein enzyme dissolve and be mixed well with meat slurry；
When using animal skeleton as enzymolysis bottom material, stock up according to the following steps：A. bone is cleaned, rejects the oil being attached on bone
Fat and meat;B. in 2-10 atmospheric pressure and 1200C-1400Add water boiling bone under the high-temperature and high-pressure conditions of C, makes into gelatine, wait for bone
After glue is cooled to the hydrolysis temperature selected needed for protease or less, it is transferred in enzymatic hydrolysis and fermentation tank, suitable table egg is added
White enzyme, stirring make edible protein enzyme dissolve and are mixed well with gelatine；Or bone is impregnated with the edible hydrochloric acid of 10-20%, make it
Calcareous dissolving simultaneously softens as loose porous bone collagen matter, then will remain in the hydrochloric acid in bone collagen matter with clear water
It rinses well, is then transferred in enzymatic hydrolysis and fermentation tank, suitable edible protein enzyme is added, stir, edible protein enzyme is made to dissolve
And it is mixed well with bone collagen matter；
（2）Fermentation enzymolysis, enzyme deactivation, filtering：Fermentation tank is put into thermostatic water bath, in the case where selecting the hydrolysis temperature needed for protease
Heat preservation enzymolysis 4-16h, obtains enzymolysis liquid；Enzymolysis liquid is transferred in rustless steel container, 10-15min is boiled, residual protein is removed to go out
The activity of enzyme；00-50C is stood for 24 hours, then filtering solution, discards filter residue to get to " functional peptide " solution；
（3）Steam distillation deodorant：By step（2）Obtained " functional peptide " solution steam distillation 1-2 hours, with removing
Fishy smell；
（4）Seasoning desensitization：In step（3）Appropriate seasoning desensitization mixture is mixed in obtained " functional peptide " solution, with elimination " work(
Slight bitter taste present in energy property peptide " solution and the thus adverse reaction of caused allergic human population.
The method that above-mentioned edible protein enzyme enzymolysis animal organ and bone prepare functional peptide food, the table egg
Enzyme includes in vain：Country allows to be used as the various protease of food additives, the protease and food used as drug for oral administration
The various protease used in processing and oral medicine manufacture craft, such as bromelain, papain, ficin
Enzyme, aspergillus oryzae, hay bacillus be mould, Aspergillus melleus, pepsin and trypsase etc..
The method that edible protein enzyme enzymolysis animal organ above-mentioned and bone prepare functional peptide food, the animal device
Official refers to the independent body with special physiological function on edible animal body, includes the brains of animal, eye, ear, larynx, nose,
The face such as tongue, the vital organs of the human body organ such as the heart, liver, lung, spleen, kidney, reproductive system, urinary system, lactation organ, pancreas, adrenal gland
Equal endocrine system organs；The animal skeleton includes each part hard bone tissue and cartilaginous tissue of animal；Described is edible
Animal includes various domestic animals, poultry, aquatic animal, two Habitat animals, reptile, the insect of artificial feeding, and country allows
The above-mentioned all kinds of animals of predation.In the small volume of edible animal, when organ is not easy to divide, animal whole body may be used and make enzyme
Solve the bottom material of liquid.
The method that edible protein enzyme enzymolysis animal organ above-mentioned and bone prepare functional peptide food, the seasoning are de-
Quick mixture is to add water to boil using the Radix Glycyrrhizae of equivalent, wrinkled giant hyssop, the root of Dahurain angelica, dried orange peel, bubble ginseng, matrimony vine, jujube and bee sugar.
The method that edible protein enzyme enzymolysis animal organ above-mentioned and bone prepare functional peptide food specifically takes pig
2, brains, are put into pot, and proper amount of clear water is added, is filtered after boiling 3min, secretes and abandon soup；The pure of 4 times of pig brain marrow weight is added
1h is boiled in water purification；It will be transferred in wall-breaking machine together with pig brain and soup, wait for that temperature is down to 500After C or less, 15 grams of bromelains are added
Enzyme, 5 grams of papains, 2 grams of ficins, machine, smash into pig brain to pieces thin pulp, and it is anti-that this thin pulp is transferred to glass
It answers in tank, then retort is put into 500Slurry after completion enzyme digestion reaction is transferred to stainless steel by heat preservation enzymolysis 6h in C hot baths
15min is boiled in pot, then uses steam distillation 2h；00C-50It is stood at a temperature of C for 24 hours, filter residue is abandoned in filtering, retains filter
Liquid mixes suitable seasoning desensitizer in filtrate, is eaten to get to " the brain peptide " for being conducive to human brain health with improving flavour of food products
Product.
The method that edible protein enzyme enzymolysis animal organ above-mentioned and bone prepare functional peptide food specifically weighs
200 grams of cock testis are put into stainless-steel pan, and appropriate tap water is added, and are boiled 3 minutes, are secreted and abandon soup；It is added the 4 of testicular weight
Pure water again keeps slightly boiling 1h, then will be transferred in wall-breaking machine together with chicken kidney and soup after boiling, wait for that temperature is down to 500After below,
10 grams of bromelains, 3 grams of papains, 1 gram of ficin is added, machines and chicken kidney is stirred into meat together with soup
Slurry, then this meat slurry is transferred in enzymatic vessel, enzymatic vessel is put into hot bath, in 500At a temperature of insulation reaction 6h；This is digested
Liquid connects slag and is transferred in another container, steam distillation 1h, by the enzymolysis liquid after this steam distillation 00C-50It is quiet at a temperature of C
It sets for 24 hours, filtering discards filter residue, is become with nourishing male reproductive function after appropriate seasoning desensitizer is mixed in filtrate
Oral " chicken kidney peptide " food.
The method that edible protein enzyme enzymolysis animal organ above-mentioned and bone prepare functional peptide food specifically will be attached
The meat and oil being connected on pig bone pick to the greatest extent, then bone is broken into the fritter that diameter is less than 10mm, impregnate bone with 15% edible hydrochloric acid
Block all dissolves out calcium phosphate therein, until bone block becomes soft as bone collagen matter grumeleuse by hard；It is more with clear water again
The secondary soaking and washing grumeleuse, removes residual acid, until washing lotion is in neutrality, obtains blocky bone collagen matter；It will be washed till neutrality again
Blocky bone collagen matter be crushed to granularity be less than 1mm particle, be put into glass jar；It is dry that bone collagen matter particle is added
Pure water that 4-8 times of weight is measured and 2% mixing protease, stir evenly, be transferred in fermentation tank, and fermentation tank is put into water-bath,
Heat-preservation fermentation 4-8 hours under conditions of 54 DEG C ± 1 DEG C；Zymotic fluid is transferred in rice steamer, steam distillation 2h；By the enzyme after distillation
Liquid is solved 00-50Under conditions of stand for 24 hours, filter residue is abandoned in filtering, retains filtrate；After appropriate seasoning desensitizer is added in filtrate
Edible bone collagen peptide；The ratio of mixing protease used is：Bromelain：Papain：Without flower
Fruit protease=6：3：1.
The type for the edible protein enzyme that can be selected in the present invention is relatively more, and current common protease includes：
Title digests pH value hydrolysis temperature
Bromelain 7.5 500C
Papain 7.5 500C
Ficin 7.5 500C
Trypsase 5-9 380C
Pepsin 1-3 380C
Intestinal proteinase 8s .0 380C
Aspergillus oryzae 4.5 500C
Novel subtilases 7.0 550C
Honey inulinase 8.0 450C
In addition, other edible protein enzymes and medical protein enzyme can also be selected as needed.When digesting animal organ, according to need
It wants, both can individually select certain edible protein enzyme, can also select two or more edible protein enzyme simultaneously.Both it can incite somebody to action
Sequence uses respectively for they, subsection enzymolysis；They can also be used in mixed way, be digested simultaneously.But if in a preparation process
When selecting two or more edible protein enzyme simultaneously in the process, either sequence uses them, subsection enzymolysis；Or it is mixed
It closes and uses them, digest simultaneously.PH conditions and temperature that each protease used needs must be all strictly observed in the case of in office
Degree condition.The dosage of protease should consider the enzyme activity of the protein content and type and protease used of bottom material
Etc. factors.
The edible animal type that can be selected is various, including：
1. livestock：Ox, donkey, horse, mule, camel, deer, all domestic animals such as exempts from and country permits the wild animal of predation at sheep.
2. poultry：All edible poultry such as chicken, duck, goose, pheasant, dove, ostrich and the national open country for allowing predation and
Bird.
3. aquatic and two Habitat animal classes：All artificial feedings such as fish, loach, swamp eel, shrimp, crab, tortoise, soft-shelled turtle, frog and country permit
Perhaps the aquatic and two Habitat animals preyed on.
4. reptile and insects：Cultivate snake and various cultivation or permit the insect of predation.
In above-mentioned various animals, for the bigger edible animal of volume, their certain part device can be individually extracted
Official either certain part skeletal tissue prepares " functional peptide " with particular physiological function, as food as fermentation bottom material
To enhance the function of human body corresponding organ.Such as：1. taking the brains of the domestic animals such as pig, sheep as bottom material, with food proteins enzyme fermentation work
Skill prepares edible " the brain peptide " for being beneficial to human brain health.2. extracting the testis of cock or the reproductive organs of male domestic animal the bottom of as
Material prepares " the kidney peptide " for being beneficial to man's reproductive function using edible protein enzyme as enzymatic hydrolysis and fermentation agent.3. extracting hen Dan Intestinal
Or it is bottom material to take the reproductive organs of female poultry, using edible protein enzyme as enzymatic hydrolysis and fermentation agent, preparation is beneficial to female reproductive system health
" benefit mother peptide ".4. to raise bone or fowl bone as bottom material, using edible protein enzyme as enzymatic hydrolysis and fermentation agent, prepares and promote green, juvenile bone
Development promotes " bone collagen peptide " of senile osteoporosis rehabilitation.5. using the heart of livestock or poultry as bottom material, with edible
Protease is enzymatic hydrolysis and fermentation agent, prepares " heart peptide " food, etc. beneficial to health of heart.In addition, for those because volume compared with
It is small, it is not readily separated the animal of special organ, its whole body can be taken to be used as fermentation bottom material.
The theoretical foundation of the present invention：Traditional theory of traditional Chinese medical science thinks that meat, skin, bone and the organ of edible animal can promote
The health of human body, and have the effect of " eating what what mends ".This theory of Traditional Chinese Medicine experienced 1,000 years and
Several hundred million person-times of practice test, it was demonstrated that it is correct in many situations, but in some cases and inefficacious.This is just meaned
It, more under cover has with human organ in the organ of animal " functional peptide " of same or similar function.Very much
In the case of, these animal organs eat belly by us, the part " functional peptide " that releases after digestion is decomposed, can be by
Absorption of human body is using enhancing the function of our corresponding organs.But in some cases, although these animal organs are eaten by us
Into belly, but decomposition cannot be digested and released " functional peptide ", thus the utilization that cannot be absorbed by the body.Using edible
Protease carries out enzymolysis processing to animal organ, so that it may complete with biochemical means would fit snugly within " functional peptide " therein
Portion releases, and is absorbed and utilized convenient for us.
Biochemistry is made that good section about the traditional theory that the indignant thought of " functional peptide " is Chinese medicine " eating what what mends "
It learns and annotates；Meanwhile how Chinese medicine should be found for modern biochemistry and to manufacture those right again about the theoretical of " eating what what mends "
There is human body certain organs the technology of " functional peptide " of special health effect to specify direction.
Present invention combination biochemistry prepares the technology and biography of biologically active " functional peptide " about enzymatic isolation method
The theory of Chinese medicine of uniting " eating what what mends " proposes the principle according to " eating what what mends ", using the organ of edible animal and bone as bottom material,
Using zymolysis technique as means, the utilization that is easily absorbed by the body can be prepared, and there is bioactivity, human health can be promoted
" functional peptide " food.
The present invention technical thought be：Suitable animal organ is selected, using suitable edible protein enzyme, suitably raw
Under the conditions of change, appropriate enzyme digestion reaction is carried out, people are released from the animal organ with specific function and are needed, with biochemistry
Activity, and can be " functional peptide " that human body is directly absorbed and utilized, as food to enhance the function of human body corresponding organ.
Biological Knowledge is taught that, during life carries out, the cell of human organ be in constantly aging and
Update.Thus we organ need constantly from blood absorb nutrition come form neoblast with replace those agings and
Dead cell.Biochemistry also teaches that cell is made of protein, and protein is made of polypeptide, polypeptide
It is made of amino acid.It is discrepant that partes corporis humani, which divides the protein of organ cell to form,.Human organ is only absorbed into
Need could to synthesize after those kinds and sufficient amount of polypeptide and amino acid the protein that needs self to form self thin
Born of the same parents go to replace those agings and dead cell, to keep the complete and healthy of organ.Otherwise, the function of organ and tissue just
It can be by serious injury.The protein of animal organ forms, closest with the composition of human body corresponding organ, by animal organ's enzyme
" functional peptide " that solution obtains also is easiest to be absorbed and utilized by the corresponding organ of human body, to meet its more neoblast, reparation group
The needs knitted.Thus it is more prone to show " effect what what mends eaten ".
In contemporary society lives, " calcium deficiency " is the phenomenon that people are from childhood to old each life span generally existing.
" calcium deficiency " can cause Juvenile development slow, the elderly's skeleton bulking, pain, the illness such as be easily broken off, thus " replenish the calcium "
Through the common requirements as popular life.Then the various medicaments replenished the calcium come into being, and type is various, and sales volume is very big.But
The effect replenished the calcium merely in many cases is not only undesirable, is easy to cause excessive calcium in our courage, kidney or bladder instead
Precipitation generates a variety of diseases such as gall stone, kidney stone, vesical calculus.Author thinks, leads to the real causes of certain people " calcium deficiency "
It is not the calcium content deficiency in food and beverage, but " the bone collagen matter " in their bones is out of joint.In fact,
Much the content of calcium ion is tantamount to ordinary person in " calcium deficiency " person's blood.Those unsound " bone collagen matter " ability with them
It is the main cause of its " calcium deficiency ".From the point of view of biochemistry, human skeleton is made of together " bone collagen matter " and calcium
's.Wherein, " bone collagen matter " is a kind of lived organic matter, and calcium is a kind of not have lived inorganic matter.At me
In body, during forming bone by " bone collagen matter " and calcium binding, " bone collagen matter ", which plays, to be dominated
Effect.The calcium ion that " the bone collagen matter " of health can initiatively draw requirement from blood generates the bone of hard health
Bone, only those unsound " bone collagen matter " can just cause the loss of calcium ion, lead to the generation of " calcium deficiency " phenomenon.Cause
And " the gelatine sustained damage in the calcium content in the top priority of " replenishing the calcium " not instead of supplements, supplement and reparation body
Crude protein.The reparation of bone collagen matter is normal, and the bone that need not replenish the calcium also can be healthy normal.Thus " bone collagen peptide "
It is only best food materials of replenishing the calcium.
The present invention is further illustrated With reference to embodiment.
Specific implementation mode
Embodiment 1：Take 2, pig brain marrow（Weigh about 200 grams）, it is put into pot, proper amount of clear water is added, is filtered after boiling 3min,
It secretes and abandons soup；4 times of pure water of pig brain marrow weight is added, boils 1h；It will be transferred in wall-breaking machine together with pig brain and soup, wait for temperature
It is down to 500After C or less, 15 grams of bromelains are added（600000 u/ grams）, 5 grams of papain, 2 grams of ficins；
It machines, smashs into pig brain to pieces thin pulp, this thin pulp is transferred in glass reaction pot, then retort is put into 500In hot bath
Heat preservation enzymolysis 6h, the slurry after completion enzyme digestion reaction is transferred in stainless-steel pan and boils 15min, with the work for removing residual protein enzyme of going out
Property.Then steam distillation 2h is used, to eliminate the fishy smell in enzymolysis product；Again by it 00C -50It is stood at a temperature of C for 24 hours,
Filtering, abandons filter residue, retains filtrate, and appropriate seasoning desensitizer is mixed in filtrate, to improve flavour of food products to get to advantageous
In " brain peptide " food of human brain health.
Embodiment 2：Weigh 200 grams of cock testis（Chicken kidney）It is put into stainless-steel pan, appropriate tap water is added, boil 3 points
Clock is secreted and abandons soup；4 times of pure water of testicular weight is added, slightly boiling 1h is kept after boiling, chicken kidney is transferred to broken wall together with soup
In machine, wait for that temperature is down to 500After C or less, 10 grams of bromelains are added（600000 u/ grams）, 3 grams of papains, 1 gram without flower
Fruit protease machines chicken kidney stirring into meat slurry together with soup, this meat slurry is transferred in enzymatic vessel, enzymatic vessel is put into hot water
In bath, the insulation reaction 6h at a temperature of 50 DEG C；This enzymolysis liquid is connected slag to be transferred in another container, steam distillation 1h, to eliminate
The fishy smell of enzymolysis product, then by this enzymolysis liquid 00C-50It is stood at a temperature of C for 24 hours, filtering discards filter residue.And in filtrate
Oral " chicken kidney peptide " food with nourishing male reproductive function is become after the appropriate seasoning desensitization mixture of incorporation.
Embodiment 3：The meat being attached on pig bone and oil are picked to the greatest extent, then bone is broken into the fritter that diameter is less than 10mm,
Bone block is impregnated with 15% edible hydrochloric acid, calcium phosphate therein is all dissolved out, until hard bone block is changed into soft gelatine
Crude protein grumeleuse；Again with the multiple soaking and washing grumeleuse of clear water, residual acid is removed, until washing lotion is in neutrality；Neutrality will be washed till
Blocky bone collagen matter is crushed to the particle that granularity is less than 1mm, is put into glass jar；It is added dry by bone collagen matter particle
Weigh 4-8 times amount pure water and 2% mixing protease, stir evenly, be transferred in fermentation tank, and fermentation tank is put into water-bath
In, heat-preservation fermentation 4-8 hours, zymotic fluid is transferred in rice steamer under conditions of 54 DEG C ± 1 DEG C, and steam distillation 2h is added suitable
After the seasoning desensitizer of amount edible bone collagen peptide；The ratio of mixing protease used is：Bromelain：
Papain：Ficin=6：3：1.
Embodiments of the present invention are not limited to the above embodiments, and that makes without departing from the purpose of the present invention is various
Within variation all belongs to the scope of protection of the present invention.
Claims (7)
- The method that 1. edible protein enzyme digests animal organ and bone prepares functional peptide food, it is characterised in that：Including following Step,（1）Stock：When using animal organ as enzymolysis bottom material, stock up according to the following steps：A. selected animal organ is cleaned After be cut into small pieces, be put into pot, tap water be added, is filtered after boiling 3-10 minute, abandons soup stock, to remove in animal meat piece spy Some fishy smell；B. the pure water of 2-6 times of animal organ's weight is added, boils 1-4h, the tight junctions of meat is destroyed, in favor of enzyme Solution reaction is smoothed out；C. meat piece is transferred to together with soup stock in wall-breaking machine, starts wall-breaking machine, meat piece is stirred into meat slurry, waits for meat After slurry is cooled to the hydrolysis temperature selected needed for protease or less, it is transferred in enzymatic hydrolysis and fermentation tank, suitable table egg is added White enzyme, stirring make edible protein enzyme dissolve and be mixed well with meat slurry；When using animal skeleton as enzymolysis bottom material, stock up according to the following steps：A. bone is cleaned, rejects the oil being attached on bone Fat and meat;B. in 2-10 atmospheric pressure and 1200C-1400Add water boiling bone under the high-temperature and high-pressure conditions of C, makes into gelatine, wait for bone After glue is cooled to the hydrolysis temperature selected needed for protease or less, it is transferred in enzymatic hydrolysis and fermentation tank, suitable table egg is added White enzyme, stirring make edible protein enzyme dissolve and are mixed well with gelatine；Or bone is impregnated with the edible hydrochloric acid of 10-20%, make it Calcareous dissolving simultaneously softens as loose porous bone collagen matter, then will remain in the hydrochloric acid in bone collagen matter with clear water It rinses well, is then transferred in enzymatic hydrolysis and fermentation tank, suitable edible protein enzyme is added, stir, edible protein enzyme is made to dissolve And it is mixed well with bone collagen matter；（2）Fermentation enzymolysis, enzyme deactivation, filtering：Fermentation tank is put into thermostatic water bath, in the case where selecting the hydrolysis temperature needed for protease Heat preservation enzymolysis 4-16h, obtains enzymolysis liquid；Enzymolysis liquid is transferred in rustless steel container, 10-15min is boiled, residual protein is removed to go out The activity of enzyme；00-50C is stood for 24 hours, then filtering solution, discards filter residue to get to " functional peptide " solution；（3）Steam distillation deodorant：By step（2）Obtained " functional peptide " solution steam distillation 1-2 hours, with removing Fishy smell；（4）Seasoning desensitization：In step（3）Appropriate seasoning desensitization mixture is mixed in obtained " functional peptide " solution, with elimination " work( Slight bitter taste present in energy property peptide " solution and the thus adverse reaction of caused allergic human population.
- 2. the method that edible protein enzyme enzymolysis animal organ according to claim 1 and bone prepare functional peptide food, It is characterized in that：The edible protein enzyme includes：Country allow as food additives various protease, as medicine for oral administration The various protease used in protease and food processing and oral medicine manufacture craft that product use, as bromelain, Papain, ficin, aspergillus oryzae, hay bacillus be mould, Aspergillus melleus, pepsin and trypsase.
- 3. the method that edible protein enzyme enzymolysis animal organ according to claim 1 and bone prepare functional peptide food, It is characterized in that：The animal organ refers to the independent body with special physiological function on edible animal body, including The brains of animal include the face of eye, ear, larynx, nose, tongue, including the heart, liver, lung, spleen, kidney the vital organs of the human body, reproductive system secretes Urinary system, including lactation organ, pancreas, adrenal internal system；Edible animal includes the various domestic animals of artificial feeding, family Fowl, aquatic animal, two Habitat animals, reptile, insect further include the above-mentioned all kinds of animals that country allows predation；Described is dynamic Object bone includes each part hard bone tissue and cartilaginous tissue of animal bodies.
- 4. the method that edible protein enzyme enzymolysis animal organ according to claim 1 and bone prepare functional peptide food, It is characterized in that：The described seasoning desensitization mixture be using the Radix Glycyrrhizae of equivalent, wrinkled giant hyssop, the root of Dahurain angelica, dried orange peel, bubble ginseng, matrimony vine, jujube and Bee sugar adds water to boil.
- 5. the method that edible protein enzyme enzymolysis animal organ according to claim 1 and bone prepare functional peptide food, It is characterized in that：2, pig brain marrow is taken, is put into pot, proper amount of clear water is added, is filtered after boiling 3min, secretes and abandon soup；Pig brain is added 4 times of pure water of marrow weight, boils 1h；It will be transferred in wall-breaking machine together with pig brain and soup, wait for that temperature is down to 500After C or less, add Enter 15 grams of bromelains, 5 grams of papains, 2 grams of ficins, machines, pig brain is smashed into pieces thin pulp, by this Thin pulp is transferred in glass reaction pot, then retort is put into 500Heat preservation enzymolysis 6h in C hot baths, after completing enzyme digestion reaction Slurry, which is transferred in stainless-steel pan, boils 15min, then uses steam distillation 2h；0 0C-5 0 CAt a temperature of stand for 24 hours, filtering, It abandons filter residue, retains filtrate, suitable seasoning desensitizer is mixed in filtrate, to improve flavour of food products to get to being conducive to people " brain peptide " food of brain health.
- 6. the method that edible protein enzyme enzymolysis animal organ according to claim 1 and bone prepare functional peptide food, It is characterized in that：It weighs 200 grams of cock testis to be put into stainless-steel pan, appropriate tap water is added, boil 3 minutes, secrete and abandon soup； 4 times of pure water of testicular weight is added, slightly boiling 1h is kept after boiling, then will be transferred in wall-breaking machine together with chicken kidney and soup, waits for temperature Degree is down to 500After below, 10 grams of bromelains, 3 grams of papains, 1 gram of ficin is added, machines chicken Kidney stirs into meat slurry together with soup, then this meat slurry is transferred in enzymatic vessel, enzymatic vessel is put into hot bath, in 500At a temperature of protect Temperature reaction 6h；This enzymolysis liquid is connected slag to be transferred in another container, steam distillation 1h exists the enzymolysis liquid after this steam distillation 00C-50It is stood at a temperature of C for 24 hours, filtering discards filter residue, mixes to become to have after seasoning desensitizer in right amount in filtrate and grow Mend oral " chicken kidney peptide " food of male reproductive function.
- 7. the method that edible protein enzyme enzymolysis animal organ according to claim 1 and bone prepare functional peptide food, It is characterized in that：The meat being attached on pig bone and oil are picked to the greatest extent, then bone is broken into the fritter that diameter is less than 10mm, with 15% Edible hydrochloric acid impregnate bone block, by calcium phosphate therein all dissolve out, until bone block by it is hard become it is soft become bone collagen Matter grumeleuse；Again with the clear water multiple soaking and washing grumeleuse, residual acid is removed, until washing lotion is in neutrality, obtains blocky bone collagen Matter；It will be washed till neutral blocky bone collagen matter again and be crushed to the particle that granularity is less than 1mm, be put into glass jar；It is added 4-8 times of pure water measured of bone collagen matter particle dry weight and 2% mixing protease, stir evenly, be transferred in fermentation tank, and Fermentation tank is put into water-bath, heat-preservation fermentation 4-8 hours under conditions of 54 DEG C ± 1 DEG C；Zymotic fluid is transferred in rice steamer, water vapour Distill 2h；By the enzymolysis liquid after distillation 00-50Under conditions of stand for 24 hours, filter residue is abandoned in filtering, retains filtrate；In filtrate Be added after appropriate seasoning desensitizer edible bone collagen peptide；The ratio of mixing protease used is：Pineapple egg White enzyme：Papain：Ficin=6：3：1.
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| CN108913749A (en) * | 2018-08-02 | 2018-11-30 | 艾苛密（上海）健康科技股份有限公司 | Active peptide nanometre collagen and preparation method thereof |
| CN109380721A (en) * | 2018-09-29 | 2019-02-26 | 内蒙古蒙肽生物工程有限公司 | A kind of comprehensive composition and preparation method thereof for adjusting treating bone diseases |
| CN109486888A (en) * | 2018-11-15 | 2019-03-19 | 铜仁市万山区水产站 | A method of extracting multifunction activity peptide from loach |
| CN109504731A (en) * | 2018-12-21 | 2019-03-22 | 河北肽都生物科技有限公司 | A kind of preparation method of Goat Placenta active peptide |
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