CN1923026A - Preparation of liver peptide - Google Patents
Preparation of liver peptide Download PDFInfo
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- CN1923026A CN1923026A CN 200610153592 CN200610153592A CN1923026A CN 1923026 A CN1923026 A CN 1923026A CN 200610153592 CN200610153592 CN 200610153592 CN 200610153592 A CN200610153592 A CN 200610153592A CN 1923026 A CN1923026 A CN 1923026A
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Abstract
The invention relates to a method for preparing liver peptide, which uses the livers of cattle and sheep as material, via secondary enzyme hydrolysis method to prepare peptide, to keep the nourishment of liver most, and remove smell, with high yield and quality.
Description
Technical field
The present invention relates to field of biological product, especially relate to a kind of preparation technology of liver peptide.
Background technology
Animal's liver is a kind of good protein nutrient source, and it contains various nutrients, digestive ferment, red blood cell, somatomedin and protein, fat, carbohydrate, vitamin and mineral matter etc., is a kind of food of vegetal pole horn of plenty.
Animal's liver also has higher medical value, is example with the beef liver, and put down in writing according to Bencao Jingshu: the beef liver hardship is sweet, and gas peace can nourish blood after edible, tonifying liver, make eye bright.Control the deficiency of blood and win thin, glaucoma, night blindness etc.According to the analytical proof to beef liver: per 100 gram beef livers contain moisture 69 grams, protein 19.8 grams, carbohydrate 9 grams, fat 3.9 grams, ash content 0.9 gram, 13 milligrams of calcium, 252 milligrams in phosphorus, 9 milligrams of iron, 5.01 milligrams on zinc, 0.39 milligram of thiamine, Riboflavin Tetrabutyrate .3 milligram.16.2 milligrams of niacins, 18 milligrams in ascorbic acid, VA2020U, VB10.16 milligram, VB21.3 milligram, VPP11.9 milligram, VC9 milligram, and contain various enzymes, phosphatide, highly unsaturated fatty acid, sweet carbon four diluted acids, cholest acid and liver glycogen etc.
In sum, animal's liver has very high nutritive value and medical value, and therefore, research and development animal's liver deep processed product will have very fine market prospects.
Summary of the invention
The object of the invention just is to provide a kind of deep processed product of animal's liver---the preparation technology of liver peptide, and this raw materials technology is easy to get, craft science, reasonable, row easy and simple to handle, easy, the efficient height, cost is low, is suitable for suitability for industrialized production.
Technical scheme of the present invention is:
A kind of preparation technology of liver peptide, its processing step is:
A, get animal's liver, remove degrease, blood impurities, when not having watery blood, use the beater homogenate through abundant washing, make meat gruel, mix with water according to 1: 2～10 ratio then, stir, the alkaline solution with 20～40% is regulated PH8.0～PH10.5, adds refining pancreatin and/or little peptide special protein enzyme 0.5 ‰～5 ‰ (V/W) at 40 ℃～65 ℃, hydrolysis 2～4 hours, time hydrolyzate of winning;
B, general hydrolyzate for the first time transfer pH value 8～10, add compound protease and carry out enzymolysis; The compound protein enzyme dosage is 0.2%～1% of the contained total protein of said mixture matter, enzymolysis time: 3～5h; The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 85～100 ℃, goes out 10～20 minutes enzyme time; The enzyme that goes out is finished rear decoloring, filtration, concentrates, is drying to obtain.
When adding pancreatin and/or little peptide special protein enzyme, press the hydrolysis time average mark and add for 2～4 times in the above-mentioned A step, each addition is 1/2～1/4 of total consumption;
When pancreatin and little peptide special protein enzyme used simultaneously, consumption respectively was 20～80%;
When adding compound protease, press the hydrolysis time average mark and add for 3～5 times in the above-mentioned B step, each addition is 1/3～1/5 of total consumption;
Need be cooled to 60～75 ℃ of row decolourings again after the described enzyme that goes out is finished, decoloring medium is active carbon and/or alukalin, consumption be in the raw material total protein 1.5～3%, 30～60 minutes time;
When above-mentioned active carbon and alukalin used simultaneously, consumption respectively was 20～80%;
Hydrolyzate after the described decolouring filters with plate and frame filter press, and filter material cloth is selected 4 * 4～8 * 8 pure cotton canvas for use, and pure cotton canvas uses after 0.2%～1% dilute alkaline soln degreasing post rinse is clean; After filtering with sand rod filter subsequently again through the smart again filter of microporous barrier;
Described drying is a spray-drying; During spray-drying, the equipment importation hot blast temperature is 130 ℃～140 ℃, and the outlet hot blast temperature is 60 ℃～90 ℃.
The present invention is that the liver with animals such as ox, sheep is a raw material, employing modern biological project method---the low temperature secondary enzyme hydrolysis prepares peptide matters, the nutritional labeling that had both kept hepatic tissue to greatest extent, removed bad smell again, and hydrolysis process is more thorough, prepared using is more abundant, product yield height, and quality is good.
Raw material of the present invention is easy to get, craft science, reasonable, and row easy and simple to handle, easy, the efficient height, cost is low, is suitable for suitability for industrialized production.
Form by 8～20 amino acid fragments by the prepared liver peptide of the inventive method, nutritious, easily digestion, and aspect the treatment and adjusting body's immunity of hepatopathys such as hepatitis B, chronic hepatitis, cirrhosis, have a good application prospect.
Modern nutriology thinks that the liver peptide molecular weight is less, easily digests and assimilates, and is very desirable enteric nutrient.Be used for spoon meat, all be well suited for for patient, old man, the children of digestion power difference.The liver peptide also can be used for health food, children, women's food, wait ooze, protein beverage, soup stock and other various food.The liver peptide is used in fish meat sausage, can improve its nutritive value, and increase emulsibility and elasticity, thereby improve mouthfeel widely, be the optimal functional ingredient of present ham sausage.
The specific embodiment
Embodiment one: (Islamic sheep liver)
Be equipped with 100 kilograms of sheep livers.
Sheep Liver Channel cold water is thawed, remove impurity such as degrease, blood, when not having watery blood, use the beater homogenate, make meat gruel through abundant washing.Mix with water according to 1: 5 ratio, stir, regulate PH8.0 with 20% sodium hydroxide solution then, little peptide special protein enzyme 0.5 ‰ (V/W) hydrolysis that adding Taiyun Biochemical Products Co., Ltd., Lingwu City produces under 40 ℃ of conditions 3 hours, time hydrolyzate of winning; When adding little peptide special protein enzyme, divide 3 addings, add 1/3 of total consumption at every turn.
Hydrolyzate is transferred pH value to 8 for the first time, and the compound protease (directly buying commodity) that safe fortune biochemical product Co., Ltd in adding Lingwu, Ningxia produces carries out enzymolysis, enzymolysis 3h.Measure protein content 2% in the hydrolyzate, protein content is 18.5% in the sheep liver, and the total protein that contains in the raw material is 18.5 kilograms, actual compound protease 92.5 grams (total protein 0.5%) that add; When adding compound protease, press the hydrolysis time average mark and add for 3 times, add 1/3 of total consumption at every turn.The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 85 ℃, goes out 10 minutes enzyme time; Going out is cooled to 60 ℃ after enzyme is finished, and adds active carbon 277.5 gram decolourings (consumption calculates according to 1.5% of total protein), 30 minutes time.Hydrolyzate after the decolouring filters with plate and frame filter press, and filter material cloth is selected 4 * 4 pure cotton canvas for use, and pure cotton canvas uses after 0.2% dilute alkaline soln degreasing post rinse is clean; Filter again through the smart filter of microporous barrier with sand rod filter behind the plate-frame filtering.After the smart filter smart filtrate is concentrated, select economic benefits and social benefits falling film type thickener for use.Concentrate the back and in the pressure spray dryer tower, carry out spray-drying; During spray-drying, control appliance import hot blast temperature is 130 ℃, and the outlet hot blast temperature is 60 ℃.
Embodiment two:
Be equipped with 100 kilograms of beef livers.
Beef liver is removed impurity such as degrease, blood, when not having watery blood, use the beater homogenate, make meat gruel through abundant washing.Mix with water according to 1: 2 ratio, stir, the sodium bicarbonate solution with 40% is regulated PH10.5, adds refining pancreatin 5 ‰ (V/W) hydrolysis 4 hours under 52 ℃ of conditions, time hydrolyzate of winning; When adding pancreatin, divide 4 addings, add 1/4 of total consumption at every turn.
Hydrolyzate is transferred pH value 9 for the first time, and the compound protease (directly buying commodity) that safe fortune biochemical product Co., Ltd in adding Lingwu, Ningxia produces carries out enzymolysis 2h.Measure protein content 2.2% in the hydrolyzate, protein content is 19.5% in the beef liver, and the total protein that contains in the raw material is 19.5 kilograms, actual compound protease 39.0 grams (0.2%) that add.When adding compound protease, press the hydrolysis time average mark and add for 2 times, add 1/2 of total consumption at every turn.The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 90 ℃, goes out 15 minutes enzyme time; Go out and be cooled to 70 ℃ after enzyme is finished, add active white pottery 390.0 gram decolourings (consumption calculates according to total protein ground 2%), 45 minutes time, hydrolyzate after the decolouring filters with plate and frame filter press, filter material cloth is selected 6 * 6 pure cotton canvas for use, and pure cotton canvas uses after 0.6% dilute alkaline soln degreasing post rinse is clean; Filter again through the smart filter of microporous barrier with sand rod filter behind the plate-frame filtering.After the smart filter smart filtrate is concentrated, select economic benefits and social benefits falling film type thickener for use.Concentrate the back and carry out spray-drying in the pressure spray dryer tower, during spray-drying, the equipment importation hot blast temperature is 140 ℃, and the outlet hot blast temperature is 70 ℃.
Embodiment three:
Be equipped with 100 kilograms of animal's livers such as sheep liver, beef liver, chicken gizzard.
Thaw through cold water, remove impurity such as degrease, blood, when not having watery blood, use the beater homogenate, make meat gruel through abundant washing.Ratio according to 1: 10 adds entry, stir, the sodium hydroxide solution with 30% is regulated PH9, adds little peptide special protein enzyme mixed enzyme 2 ‰ (V/W) that refining pancreatin and Taiyun Biochemical Products Co., Ltd., Lingwu City produce under 65 ℃ of conditions, hydrolysis 3 hours, time hydrolyzate of winning; When adding pancreatin and little peptide special protein enzyme mixed enzyme, divide 3 addings, add 1/3 of total consumption at every turn, wherein the little peptide special protein enzyme of pancreatin and Taiyun Biochemical Products Co., Ltd., Lingwu City production is respectively 20% and 80%.
Hydrolyzate is transferred pH value 10 for the first time, and the compound protease (directly buying commodity) that safe fortune biochemical product Co., Ltd in adding Lingwu, Ningxia produces carries out enzymolysis 5h.Measure protein content 2.1% in the hydrolyzate, protein in liver content is 19%, and the total protein that contains in the raw material is 19 kilograms, actual compound protease 190 grams (total protein 1%) that add.When adding compound protease, press the hydrolysis time average mark and add for 5 times, add 1/5 of total consumption at every turn.The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 100 ℃, goes out 20 minutes enzyme time; Going out is cooled to 75 ℃ after enzyme is finished, and adds active carbon and alukalin 380 gram decolourings (consumption calculates according to total protein ground 2%), and 60 minutes time, wherein active carbon 76 restrains, alukalin 304 grams.Hydrolyzate after the decolouring filters with plate and frame filter press, and filter material cloth is selected 8 * 8 pure cotton canvas for use, and pure cotton canvas uses after 1% dilute alkaline soln degreasing post rinse is clean; Filter again through the smart again filter of microporous barrier with sand rod filter behind the plate-frame filtering.After the smart filter smart filtrate is concentrated, select economic benefits and social benefits falling film type thickener for use.Concentrate the back and carry out spray-drying in the pressure spray dryer tower, during spray-drying, the equipment importation hot blast temperature is 130 ℃, and the outlet hot blast temperature is 90 ℃.
Embodiment four:
With embodiment three, during the little peptide special protein enzyme mixed enzyme that adds that pancreatin and Taiyun Biochemical Products Co., Ltd., Lingwu City produce, its consumption is respectively 50% and 50%.
When adding active carbon and alukalin during decolouring, its consumption is respectively 50% and 50%.
Embodiment five:
With embodiment three, during the little peptide special protein enzyme mixed enzyme that adds that pancreatin and Taiyun Biochemical Products Co., Ltd., Lingwu City produce, its consumption is respectively 80% and 20%.
When adding active carbon and alukalin during decolouring, its consumption is respectively 80% and 20%.
The preparation method of embodiment three, four, five and index are equally applicable among embodiment one and the embodiment two preparation and use pancreatin and little peptide special protein enzyme the time during hydrolyzate for the first time, and use active carbon and alukalin the time during decolouring.
Dry back can have soapy feeling according to user's request behind ultramicro grinding 300～1200 orders, easilier absorbed by human body.
Claims (8)
1, a kind of preparation technology of liver peptide, its processing step is:
A, get animal's liver, remove degrease, blood impurities, when not having watery blood, use the beater homogenate through abundant washing, make meat gruel, mix with water according to 1: 2～10 ratio then, stir, the alkaline solution with 20～40% is regulated PH8.0～PH10.5, adds refining pancreatin and/or little peptide special protein enzyme 0.5 ‰～5 ‰ (V/W) at 40 ℃～65 ℃, hydrolysis 2～4 hours, time hydrolyzate of winning;
B, general hydrolyzate for the first time transfer pH value 8～10, add compound protease and carry out enzymolysis; The compound protein enzyme dosage is 0.2%～1% of the contained total protein of said mixture matter, enzymolysis time: 3～5h; The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 85～100 ℃, goes out 10～20 minutes enzyme time; The enzyme that goes out is finished rear decoloring, filtration, concentrates, is drying to obtain.
2, technology as claimed in claim 1 is characterized in that: when adding pancreatin and/or little peptide special protein enzyme, press the hydrolysis time average mark and add for 2～4 times in the above-mentioned A step, each addition is 1/2～1/4 of total consumption.
3, technology as claimed in claim 1 or 2 is characterized in that: when pancreatin and little peptide special protein enzyme used simultaneously, consumption respectively was 20～80%.
4, technology as claimed in claim 1 is characterized in that: when adding compound protease, press the hydrolysis time average mark and add for 3～5 times in the above-mentioned B step, each addition is 1/3～1/5 of total consumption.
5, technology as claimed in claim 1 is characterized in that: need be cooled to 60～75 ℃ of row decolourings again after the described enzyme that goes out is finished, decoloring medium is active carbon and/or alukalin, consumption be in the raw material total protein 1.5～3%, 30～60 minutes time.
6, as claim 1 or 5 described technologies, it is characterized in that: when above-mentioned active carbon and alukalin used simultaneously, consumption respectively was 20～80%.
7, technology as claimed in claim 1 is characterized in that: the hydrolyzate after the described decolouring filters with plate and frame filter press, and filter material cloth is selected 4 * 4～8 * 8 pure cotton canvas for use, and pure cotton canvas uses after 0.2%～1% dilute alkaline soln degreasing post rinse is clean; After filtering with sand rod filter subsequently again through the smart again filter of microporous barrier.
8, technology as claimed in claim 1 is characterized in that: described drying is a spray-drying; During spray-drying, the equipment importation hot blast temperature is 130 ℃～140 ℃, and the outlet hot blast temperature is 60 ℃～90 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610153592 CN1923026A (en) | 2006-09-13 | 2006-09-13 | Preparation of liver peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610153592 CN1923026A (en) | 2006-09-13 | 2006-09-13 | Preparation of liver peptide |
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| CN1923026A true CN1923026A (en) | 2007-03-07 |
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| CN 200610153592 Pending CN1923026A (en) | 2006-09-13 | 2006-09-13 | Preparation of liver peptide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101301314B (en) * | 2008-06-26 | 2011-06-29 | 江苏克胜药业有限公司 | Technique for producing liver extract |
| CN101613735B (en) * | 2009-08-17 | 2011-08-24 | 张吉越 | Method for preparing organic polypeptide |
| CN108208496A (en) * | 2016-12-12 | 2018-06-29 | 锡林郭勒盟肽好生物制品有限责任公司 | The preparation method of blood sugar lowing polypeptide draft solid beverage and liver Gly-His-Lys |
| CN108308620A (en) * | 2018-03-27 | 2018-07-24 | 张至德 | The method that edible protein enzyme digests animal organ and bone prepares functional peptide food |
| CN108813636A (en) * | 2018-09-18 | 2018-11-16 | 广东羲准生物科技有限公司 | Beneficial anahemin composition and its application |
| CN109371089A (en) * | 2018-12-25 | 2019-02-22 | 河北肽都生物科技有限公司 | A kind of extracting method of small molecule liver peptide |
| CN111772188A (en) * | 2020-07-07 | 2020-10-16 | 河北智同生物制药股份有限公司 | Liver polypeptide for protecting liver and improving liver function and composition thereof |
-
2006
- 2006-09-13 CN CN 200610153592 patent/CN1923026A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101301314B (en) * | 2008-06-26 | 2011-06-29 | 江苏克胜药业有限公司 | Technique for producing liver extract |
| CN101613735B (en) * | 2009-08-17 | 2011-08-24 | 张吉越 | Method for preparing organic polypeptide |
| CN108208496A (en) * | 2016-12-12 | 2018-06-29 | 锡林郭勒盟肽好生物制品有限责任公司 | The preparation method of blood sugar lowing polypeptide draft solid beverage and liver Gly-His-Lys |
| CN108308620A (en) * | 2018-03-27 | 2018-07-24 | 张至德 | The method that edible protein enzyme digests animal organ and bone prepares functional peptide food |
| CN108813636A (en) * | 2018-09-18 | 2018-11-16 | 广东羲准生物科技有限公司 | Beneficial anahemin composition and its application |
| CN109371089A (en) * | 2018-12-25 | 2019-02-22 | 河北肽都生物科技有限公司 | A kind of extracting method of small molecule liver peptide |
| CN111772188A (en) * | 2020-07-07 | 2020-10-16 | 河北智同生物制药股份有限公司 | Liver polypeptide for protecting liver and improving liver function and composition thereof |
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