CN106260497A - A kind of giant salamander nourishing egg albumen powder production technology - Google Patents
A kind of giant salamander nourishing egg albumen powder production technology Download PDFInfo
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- CN106260497A CN106260497A CN201610648999.XA CN201610648999A CN106260497A CN 106260497 A CN106260497 A CN 106260497A CN 201610648999 A CN201610648999 A CN 201610648999A CN 106260497 A CN106260497 A CN 106260497A
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- China
- Prior art keywords
- andrias davidianus
- davidianus blanchard
- powder
- giant salamander
- dried
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
Abstract
The present invention relates to a kind of with Andrias davidianus Blanchard as raw material, use the method that enzymatic hydrolysis makes liquefaction Andrias davidianus Blanchard egg albumen powder.The method puts into defat in extraction pot after Andrias davidianus Blanchard is made Andrias davidianus Blanchard powder, then the Andrias davidianus Blanchard powder after defat is added appropriate water and neutral protease is hydrolyzed, filters, then carry out filtrate being concentrated in vacuo, being spray-dried, i.e. obtain the Andrias davidianus Blanchard egg albumen powder that liquefies.This product is soluble in water, containing 20 several amino acids and several mineral materials and trace element, and essential amino acids composition is comprehensively, rich content, can be used for manufacturing infant nutrition Andrias davidianus Blanchard paste, fully nutrient, amino acid gelatin pill, animal-plant kind mixed protein powder also may be utilized in fabricating high-grade nutrient flavouring agent.
Description
Technical field
The method that the present invention relates to proteolysis, relates to the deep processing of Andrias davidianus Blanchard.
Background technology
Hydrolyzed protein, should be more than the 50% of total nitrogen (N) containing amino nitrogen.Required amino is provided for body anabolism
Acid, to maintain internal nitrogen budgets.For malnutrition, because of protein digestibility malabsorption or consume excessively caused protein lack
Weary, severe gastro-enteritis and scald or the Protein intake etc. of surgical site infections, have medicine, nutrition aspect purposes widely.Mesh
Before, the hydrolyzed protein of China is mainly with the vegetable protein such as soybean meal, Semen arachidis hypogaeae dregs or animal blood as raw material, and abroad such as day
This, the U.S. use the aquatic products in ocean or abyssal fish to be that the existing three kinds of forms of hydrolyzed protein made by raw material, i.e. FPC,
Function fish protein and liquefaction fish protein.
At present, the method for hydrolysis of protein mainly has alkali process hydrolysis, Acid hydrolysis and enzymatic hydrolysis.Although alkali process hydrolysis water
Solution rate is higher, but most of amino acid whose structure and configuration are destroyed, and lose physiological action.Although Acid hydrolysis does not destroy structure
Type, but tryptophan is unstable to acid, and Acid hydrolysis need to neutralize with sodium hydroxide, produces a large amount of sodium chloride, had both made desalination, ammonification
Sodium content the highest (5%), limits its range of application.Biological enzyme hydrolysis makes egg albumen powder and Comparatively speaking overcomes above-mentioned lacking
Point.
Andrias davidianus Blanchard (Andrias davidianus Blanchard) popular name " Megalobatrachus japonicus daoidianuas (Blanchard) ", belongs to Amphibia, Caudata, the hidden gill
Salamander section, belongs to country second class protection animal.Along with the increase of artificial cultivation quantity, the secondary fish of parton allows to develop, for
Industrialization development provides the foundation condition.
The traditional Chinese medical science is thought, Andrias davidianus Blanchard property is sweet, flat, lightly seasoned, have QI invigorating, nourish blood, Fructus Alpiniae Oxyphyllae, nourishing, strong function.Cure mainly nerve to decline
Weak, anemia, dysentery, malaria.Modern medicine is thought, often eating can be with clever Fructus Alpiniae Oxyphyllae, slow down aging, raising hemopoietic and immunity merit
Can, there is well effect to preventing cardio-cerebrovascular, pernicious anemia and malignant tumor.Modern science is thought: Andrias davidianus Blanchard is meat
Animal, its meat is delicate, delicious flavour, rich in having high quality protein, 17 kinds of aminoacid, 8 kinds of essential amino acids, the natural work of kind more than 50
Property material and abundant collagen protein, cell microcirculation can be improved, promote protein synthesis, to nourishing human body, enhancing immunity,
Slow down aging has special effect.In its muscle, EAAI (essential amino acid index) is more than beef and Carnis Cervi, to human nutrition and health
The most useful.
Therefore, for Raw material processing egg albumen powder, there is good prospect and value with Andrias davidianus Blanchard.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing biological enzyme hydrolysis to make liquefaction Andrias davidianus Blanchard egg albumen powder, made
Liquefaction Andrias davidianus Blanchard egg albumen powder is the most soluble in water, and rich in aminoacid and polypeptide and several mineral materials, trace element.
For achieving the above object, the technical solution adopted in the present invention is: a kind of giant salamander nourishing egg albumen powder producer
Method, follows these steps to carry out:
Powder process: Andrias davidianus Blanchard uses the vexed method of heat slaughter, then removes internal organs, cold wind is dried and is made just Andrias davidianus Blanchard powder processed；
Defat: Andrias davidianus Blanchard powder is put in extraction pot, by weight for Andrias davidianus Blanchard powder: atent solvent 1: 1～1.5 adds atent solvent
Carrying out defat, degreasing time 0.5～1 hour, temperature controls below 100 DEG C；
Hydrolysis: the Andrias davidianus Blanchard powder after defat is added water and neutral protease is hydrolyzed, filters；The water quality wherein added
For 7～10 times of Andrias davidianus Blanchard powder, neutral protease weight is the 0.3～0.8% of solid content weight, hydrolysis temperature 45～80 DEG C, time
Between 3～9 hours；
Purify: the filtrate after filtering add activated carbon carry out decolouring, deodorize, de-abnormal flavour；
Concentrate: the filtrate after decoloration and deodorization being placed in vacuum and being concentrated into solid content is 40～45% only；
It is spray-dried: concentrated solution is spray-dried, i.e. can be made into white or light luteofuscous water solublity Andrias davidianus Blanchard egg albumen powder.
Preferably, described Andrias davidianus Blanchard goes internal organs to be carried out after slaughtering, blends, and then cold wind is dried and made just Andrias davidianus Blanchard powder processed；
Or cold wind smashs into just Andrias davidianus Blanchard powder processed to pieces after drying.
Preferably, in described milling step, Andrias davidianus Blanchard liver, blood, stomach cold wind are dried, smash into just Andrias davidianus Blanchard powder processed to pieces one
Point.
Preferably, in described purification process, activated carbon dosage is 70～75% of solid content in filtrate.
Preferably, in described dry run, concentrated solution is dried to product moisture content through pressure spray dryer case and is less than
0.3%.
Preferably, described atent solvent is one or both in petroleum ether, hexamethylene, ethyl acetate.
Preferably, vexed the slaughtering of described heat is to mix fumigation heating with Folium Artemisiae Argyi and Radix Glycyrrhizae herb by the weight of 3:1, and will be big
Salamander be placed in directly over and sultry lethal.
The method have the advantages that the feature that the present invention is directed to Andrias davidianus Blanchard raw material, use compound protease to carry out enzyme
Solve, degree of hydrolysis can be effectively improved.Product has aromatic flavor, without the feature such as fishy smell and bitterness.Employing heat is vexed slaughters, maximum journey
Degree retains the nutritive values such as Andrias davidianus Blanchard blood, it is ensured that its nutritional labeling does not runs off.The most relatively electric shock is slaughtered and Andrias davidianus Blanchard can be avoided to be subject to
Muscle, blood etc. is caused to produce stress and harmful substance to extraneous strong stimulation.And sultry high temperature can improve Andrias davidianus Blanchard
Metabolism, stimulates Andrias davidianus Blanchard human body energy to be in stronger state, as the quality of medicinal raw material, Andrias davidianus Blanchard is had positive effect.And
It is also to have benefit the most medically to animal that Radix Glycyrrhizae, Folium Artemisiae Argyi itself fumigate itself.Prior art is just frequently utilized that Radix Glycyrrhizae
As the medicine of the mediation property of medicine, Folium Artemisiae Argyi is utilized to carry out moxa-moxibustion etc..Although mechanism is not fully understood at present, but use side of the present invention
The Andrias davidianus Blanchard that method is slaughtered, all has a lot of significantly advantage putting into practice medicinal aspect.Blood pressure lowering is especially had solely by the product of the present invention
Special benefit.Hyperpietic suitable for 30 conditions is randomly divided into two groups, and experimental group takes the embodiment of the present invention one every day
Egg albumen powder product 15g, takes with meal in three times, and after 3 weeks, experimenter's blood pressure full recovery is normal.Even and if by same technique
And edible way, but electric shock is used to slaughter；15 experimenters only have 4 people's blood pressures and recover normal, also have 5 human bloods in remaining 11 people
Pressure is without significant change.
Utilize the Andrias davidianus Blanchard egg albumen powder that the inventive method is processed, soluble in water, it is of high nutritive value, containing more than 20 kinds of amino
Acid, it is necessary to aminoacid composition is comprehensive, rich content.And containing several mineral materials and trace element, dissolution characteristics (solubility,
Wettability, dispersivity and dissolution velocity) and product special flavour good, can be used for manufacture infant nutrition fish paste, fully nutrient, aminoacid glue
Ball, sports drink etc..Andrias davidianus Blanchard is again a kind of traditional famous and precious edible medicinal animal, high nutritive value, nutrition more fully, easily
In absorption of human body, have regulation function of human body, improve immunity, lowering blood pressure and blood fat, improvement sleep, slow down aging, beauty treatment support
Face, prophylaxis of tumours, promotion such as breed at the multiple efficacies, are suitable for all groups and use.
Detailed description of the invention
For making those skilled in the art more understand embodiments of the present invention and beneficial effect, it is provided that following be preferable to carry out
Example.
Embodiment one
First the Andrias davidianus Blanchard vexed method of heat being slaughtered, concrete operations are exactly to use dry Folium Artemisiae Argyi and Radix Glycyrrhizae herb by the weight of 3:1
Mixing fumigation heating, and sultry lethal directly over Andrias davidianus Blanchard is placed in.Then going internal organs to be carried out, blend, then cold wind dries
Andrias davidianus Blanchard powder processed at the beginning of drying one-tenth.Again Andrias davidianus Blanchard powder is put in extraction pot, adds the petroleum ether solvent of equivalent, temperature control 80～
100 DEG C, extraction time, at 1 hour, obtains defat Andrias davidianus Blanchard powder.
Again the Andrias davidianus Blanchard powder after defat is put in hydrolysis cylinder, adds the water being equivalent to 9 times of weight of Andrias davidianus Blanchard powder, stir,
Making temperature rise to 60～70 DEG C, the indeterminate plant protease adding solid content weight 0.5% is hydrolyzed, and hydrolysis time is 6 little
Time, it is warmed up to 85 DEG C of enzyme denaturing, filtrations the most again.
Filtrate after filtering is squeezed in bleaching kettle, adds activated carbon, then heats to 90～95 DEG C, maintain the temperature at 1
Hour, then it is down to 45 DEG C of filtrations.During wherein activated carbon dosage is filtrate the 75% of solid content.
Filtrate after decoloration and deodorization being put in vacuum concentration pan again and concentrate, being concentrated into solid content is 45% only.Dense
Vacuum during contracting is 0.08MPa, and steam pressure is 0.15MPa.
Finally with pressure spray dryer case, concentrated solution is spray-dried；Atomisation pressure 15MPa, inlet temperature 150 DEG C, air draft
Temperature 80 DEG C, drying baker temperature 85 DEG C.The water solublity Andrias davidianus Blanchard albumen of moisture content 0.3% is i.e. obtained after the spray drying of above-mentioned condition
Powder.Based on just Andrias davidianus Blanchard powder processed, final water-solubility protein powder yield reaches 68.1%.
Embodiment two
First the Andrias davidianus Blanchard vexed method of heat being slaughtered, concrete operations are exactly to mix by the weight of 3:1 with Folium Artemisiae Argyi and Radix Glycyrrhizae herb
Fumigation generates heat, and sultry lethal directly over being placed in by Andrias davidianus Blanchard.Then going internal organs to be carried out, blend, then cold wind dries system
Become Andrias davidianus Blanchard powder.Putting in extraction pot by Andrias davidianus Blanchard powder again, the hexamethylene adding equivalent makees solvent, and temperature controls, at 80～100 DEG C, to carry
The time that takes, at 0.5 hour, obtains defat Andrias davidianus Blanchard powder.
Again the Andrias davidianus Blanchard powder after defat is put in hydrolysis cylinder, adds the water being equivalent to 8 times of weight of Andrias davidianus Blanchard powder, stir,
Making temperature rise to 60～70 DEG C, the indeterminate plant protease adding solid content weight 0.7% is hydrolyzed, and hydrolysis time is 8 little
Time, it is warmed up to 80 DEG C of enzyme denaturing, filtrations the most again.
Filtrate after filtering is squeezed in bleaching kettle, adds activated carbon, then heats to 90～95 DEG C, maintain the temperature at
0.5 hour, then it is down to 50 DEG C of filtrations.During wherein activated carbon dosage is filtrate the 70% of solid content.
Filtrate after decoloration and deodorization being put in vacuum concentration pan again and concentrate, being concentrated into solid content is 40% only.Dense
Vacuum during contracting is 0.1MPa, and steam pressure is 0.1MPa.
Finally with pressure spray dryer case, concentrated solution is spray-dried；Atomisation pressure 12MPa, inlet temperature 150 DEG C, air draft
Temperature 80 DEG C, drying baker temperature 85 DEG C.The water solublity Andrias davidianus Blanchard albumen of moisture content 0.3% is i.e. obtained after the spray drying of above-mentioned condition
Powder.Based on just Andrias davidianus Blanchard powder processed, final water-solubility protein powder yield reaches 66.8%.
Embodiment three
First the Andrias davidianus Blanchard vexed method of heat being slaughtered, concrete operations are exactly to mix by the weight of 3:1 with Folium Artemisiae Argyi and Radix Glycyrrhizae herb
Fumigation generates heat, and sultry lethal directly over being placed in by Andrias davidianus Blanchard.Then going internal organs to be carried out, blend, then cold wind dries system
Become Andrias davidianus Blanchard powder.Putting in extraction pot by Andrias davidianus Blanchard powder again, the petroleum ether adding equivalent makees solvent with ethyl acetate equal amount of mixture, temperature
Degree controls at 80～100 DEG C, and extraction time, at 0.75 hour, obtains defat Andrias davidianus Blanchard powder.
Again the Andrias davidianus Blanchard powder after defat is put in hydrolysis cylinder, adds the water being equivalent to 8 times of weight of Andrias davidianus Blanchard powder, stir,
Making temperature rise to 60～70 DEG C, the indeterminate plant protease adding solid content weight 0.4% is hydrolyzed, and hydrolysis time is 8 little
Time, it is warmed up to 85 DEG C of enzyme denaturing, filtrations the most again.
Filtrate after filtering is squeezed in bleaching kettle, adds activated carbon, then heats to 90～95 DEG C, maintain the temperature at
0.75 hour, then it is down to 45 DEG C of filtrations.During wherein activated carbon dosage is filtrate the 75% of solid content.
Filtrate after decoloration and deodorization being put in vacuum concentration pan again and concentrate, being concentrated into solid content is 40% only.Dense
Vacuum during contracting is 0.1MPa, and steam pressure is 0.12MPa.
Finally with pressure spray dryer case, concentrated solution is spray-dried；Atomisation pressure 15MPa, inlet temperature 150 DEG C, air draft
Temperature 80 DEG C, drying baker temperature 85 DEG C.The water solublity Andrias davidianus Blanchard albumen of moisture content 0.3% is i.e. obtained after the spray drying of above-mentioned condition
Powder.Based on just Andrias davidianus Blanchard powder processed, final water-solubility protein powder yield reaches 70.8%.
Claims (7)
1. a giant salamander nourishing process for producing protein powder, it is characterised in that: follow these steps to carry out:
Powder process: Andrias davidianus Blanchard uses the vexed method of heat slaughter, then removes internal organs, cold wind is dried and is made just Andrias davidianus Blanchard powder processed；
Defat: Andrias davidianus Blanchard powder is put in extraction pot, by weight for Andrias davidianus Blanchard powder: atent solvent 1: 1～1.5 adds atent solvent to be carried out
Defat, degreasing time 0.5～1 hour, temperature controls below 100 DEG C；
Hydrolysis: the Andrias davidianus Blanchard powder after defat is added water and neutral compound protease is hydrolyzed, filters；The water quality wherein added
For 7～10 times of Andrias davidianus Blanchard powder, neutral protease weight is the 0.3～0.8% of solid content weight, hydrolysis temperature 45～80 DEG C, time
Between 3～9 hours；
Purify: the filtrate after filtering add activated carbon carry out decolouring, deodorize, de-abnormal flavour；
Concentrate: the filtrate after decoloration and deodorization being placed in vacuum and being concentrated into solid content is 40～45% only；
It is spray-dried: concentrated solution is spray-dried, i.e. can be made into white or light luteofuscous water solublity Andrias davidianus Blanchard egg albumen powder.
Giant salamander nourishing process for producing protein powder the most according to claim 1, it is characterised in that: in described Andrias davidianus Blanchard goes after slaughtering
Dirty being carried out, blend, then cold wind is dried and is made just Andrias davidianus Blanchard powder processed；Or cold wind smashs into just Andrias davidianus Blanchard powder processed to pieces after drying.
Giant salamander nourishing process for producing protein powder the most according to claim 1, it is characterised in that: in described purification process, live
Property charcoal consumption is 70～75% of solid content in filtrate.
Giant salamander nourishing process for producing protein powder the most according to claim 1, it is characterised in that: in described dry run, will
Concentrated solution is dried to product moisture content less than 0.3% through pressure spray dryer case.
Giant salamander nourishing process for producing protein powder the most according to claim 1, it is characterised in that: described atent solvent is stone
Oil ether, hexamethylene, ethyl acetate in one or both.
Giant salamander nourishing process for producing protein powder the most according to claim 1, it is characterised in that: in described milling step,
Andrias davidianus Blanchard liver, blood, stomach cold wind are dried, smashs into a part for just Andrias davidianus Blanchard powder processed to pieces.
Giant salamander nourishing process for producing protein powder the most according to claim 1, it is characterised in that: vexed the slaughtering of described heat is to use
Folium Artemisiae Argyi and Radix Glycyrrhizae herb press the weight mixing fumigation heating of 3:1, and sultry lethal directly over being placed in by Andrias davidianus Blanchard.
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| CN201610648999.XA CN106260497A (en) | 2016-08-09 | 2016-08-09 | A kind of giant salamander nourishing egg albumen powder production technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107927181A (en) * | 2017-07-04 | 2018-04-20 | 迪庆香格里拉舒达有机食品有限公司 | Walnut Milk that suitable middle-aged and elderly people drinks and preparation method thereof |
| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361393A (en) * | 2013-07-29 | 2013-10-23 | 四川龙王洞生态农业开发有限公司 | Preparation method of andrias oligopeptide |
| CN103493969A (en) * | 2013-07-29 | 2014-01-08 | 四川龙王洞生态农业开发有限公司 | Method for producing giant salamander polypeptide powder |
-
2016
- 2016-08-09 CN CN201610648999.XA patent/CN106260497A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361393A (en) * | 2013-07-29 | 2013-10-23 | 四川龙王洞生态农业开发有限公司 | Preparation method of andrias oligopeptide |
| CN103493969A (en) * | 2013-07-29 | 2014-01-08 | 四川龙王洞生态农业开发有限公司 | Method for producing giant salamander polypeptide powder |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107927181A (en) * | 2017-07-04 | 2018-04-20 | 迪庆香格里拉舒达有机食品有限公司 | Walnut Milk that suitable middle-aged and elderly people drinks and preparation method thereof |
| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
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Application publication date: 20170104 |