CN106937731A - Queen bee nit powder extracts and its preparation method and application - Google Patents
Queen bee nit powder extracts and its preparation method and application Download PDFInfo
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- CN106937731A CN106937731A CN201610005286.1A CN201610005286A CN106937731A CN 106937731 A CN106937731 A CN 106937731A CN 201610005286 A CN201610005286 A CN 201610005286A CN 106937731 A CN106937731 A CN 106937731A
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- queen bee
- bee larva
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- extract
- larva extract
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The invention discloses queen bee nit powder extracts and its preparation method and application.The invention provides a kind of preparation method of queen bee larva extract I, including step 1：Queen bee larva, adds volumetric concentration and is extracted for 90%~95% ethanol water, filtering obtains filtrate A；Step 2：Mixed after filtrate A is concentrated with low polar solvent and solubilizer, obtain mixed liquid B, described low polar solvent is glycolic solvents；Step 3：Mixed liquid B is mixed with water, sterilized, queen bee larva extract I is obtained.The preparation method of the present invention can prepare three kinds of queen bee nit powder extracts, respectively containing abundant liposoluble vitamin, abundant amino acid, peptide matters or chitin in these extracts, cosmetics, health care of food product and breeding feed industry are can be applied to, with highly important economic worth and social value.
Description
Technical field
The present invention relates to queen bee nit powder extracts and its preparation method and application.
Background technology
No matter now most foster honeybee persons, support and support few more, raises or chases after flower surely and take honey by force, income is starched in production
The ratio for accounting for bee product total income is increasing, especially supports slurry honeybee.The simple group of present royal jelly is produced per year
Amount has broken through 10kg, and the annual production of China's royal jelly is more than 3000 tons, according to every production 1kg royal jelly,
Queen bee nit 0.2kg~0.3kg can typically be produced to calculate, 600 tons~900 tons of queen bee nit is produced in the whole nation per year.
However, the annual queen bee nit for being used to process in the whole nation only has 50 tons or so, it is remaining all by bee keeper
Reason is fallen, and this is surprising waste.
The queen bee larva powder of freeze-drying contains protein about 48%, fat 15%, amino acid classes and honeybee
Royal jelly is basically identical, includes eight kinds of 18 kinds of main amino acid, and lysine etc. needed by human
Amino acid also extremely enriches, and is a kind of full price albumen.The content of queen bee nit vitamin is also very abundant, especially
It is vitamin C and vitamin D.As can be seen here, queen bee larva has high nutritive value.Its
Tangible external someone very early utilizes queen bee nit, and the Germanic nationality in Europe is considered as it indispensable in wedding dinner
Resident on food, Mexico You Kadan islands mixes and stirs larva of bee, salt and lemon juice to be recruited as ticbit
Honored guest is treated, Japan is also utilized to make various nutraceutical and beverage in recent years.But, remove at home
Indivedual areas such as Mojiang, yunnans, Wan Ping, dynamic company resident always assign the larva of bee of delicious flavour as U.S.
Taste delicacies.
Although in recent years with the pursuit to pollution-free food, to health care and the intensification of aging understanding and with swelling
Bee product is set to enjoy favor during knurl, disease fight, but it is no matter domestic or external, it is main or thick
The larva of shallow exploitation honeybee and pupa, converted products roughly, the activity in the honeybee larve and pupae made
Composition can not be effectively maintained and be fully absorbed utilization by human body.
Patent CN1090977A (bee pupa series nutriment and preparation method thereof) disclose a kind of enzymolysis and
The method that nutriment in bee pupa is extracted in lactic fermentation, then adds excipient cane sugar powder, milk powder wherein
And soluble starch pelletize and obtains bee pupa nutritional granular instant or add water wherein, emulsify
Agent etc. adjust taste obtain nutrition oral administration, be finally separating it is residue obtained can be as animal feed additive.
Patent CN1273786A (a kind of preparation method of bee larva product) discloses a kind of using enzymolysis,
Then using the method for addition natural polymeric surface active agent, prior bee larva active ingredient is first in alcohol
Overcritical concentration in aqueous solvent is extracted, and then using ion exchange resin adsorption and purification, obtains extract.
However, the method disclosed in patent CN1090977A and patent CN1273786A is by certain
Technology means carry out extraction separation to certain class predetermined substance in queen bee larva, fail to realize to honeybee
The full components utilising of king's larva powder.
From the above, it is seen that the deep processing at present to the product of bee larva is also more extensive, not
Various nutritional ingredients of different nature therein are separated.Regarding to the issue above, queen bee nit is opened up
Development of many application studies to promotion apiculture, turns waste into wealth, benefits people, developing queen bee children
There are highly important realistic meaning and long-range economic implications in this high-protein nutrient source of worm powder.
The content of the invention
The technical problems to be solved by the invention are to overcome the product of bee larva in the prior art
The defect such as processing technology is not fine, nutriment can not make full use of and there is provided queen bee nit powder extracts
And its preparation method and application.The preparation method of the present invention can prepare three kinds of queen bee nit powder extracts,
Respectively containing abundant liposoluble vitamin, abundant amino acid, peptide matters or shell in these extracts
Polysaccharide, can be applied to cosmetics, health care of food product and breeding feed industry, with highly important economy
Value and social value.The preparation method of the present invention realizes the full components utilising to queen bee larva, carries
The high economic worth of queen bee larva.
The invention provides a kind of preparation method of queen bee larva extract I, it comprises the following steps：
Step 1：Queen bee larva, adds volumetric concentration and is extracted for 90%~95% ethanol water,
Filtering, obtains filtrate A, described volumetric concentration refers to that the volume of ethanol accounts for ethanol water cumulative volume
Percentage；
Step 2：Mix, obtain with low polar solvent and solubilizer after the filtrate A concentrations that step 1 is obtained
To mixed liquid B, described low polar solvent is glycolic solvents；
Step 3：The mixed liquid B that step 2 is obtained mixes with water, sterilized, and obtains queen bee larva and carries
Take thing I.
In step 1, described queen bee larva can be queen bee larva conventional in the art, preferably
Protein is more than 50%, and ash content is less than 5% queen bee larva.
In step 1, described volumetric concentration is that 90%~95% ethanol water and described queen bee are young
Volume mass the ratio preferred 3mL/g~5mL/g, such as 4mL/g of worm powder.
In step 1, described extracting can use the conventional method of the generic operation in this area, described
Preferably 40 DEG C~60 DEG C, such as 50 DEG C of the temperature of extraction；The time of described extraction preferably 2 hours~3
Hour.
In step 1, described filtering can use the conventional method of the generic operation in this area, preferably subtract
Press filtration.
In step 2, described concentration can be the conventional method of the generic operation in this area, described filter
The preferred 0.3mL/g of volume mass ratio of volume after liquid A concentrations and described queen bee larva~
0.6mL/g, such as 0.4mL/g.
In step 2, the preferred propane diols of described glycolic solvents, butanediol and one kind in pentanediol or
It is a variety of.
In step 2, described glycolic solvents and the volume mass of the queen bee larva described in step 1
Ratio preferred 0.008mL/g~0.012mL/g, such as 0.01mL/g.
In step 2, described solubilizer can be polyethylene glycol hydrogenated castor conventional in cosmetic field
Oils solubilizer, preferably PEG-30 rilanit specials, PEG-35 rilanit specials, PEG-40 hydrogenations
Castor oil (CO40), PEG-45 rilanit specials and one kind or many in PEG-50 rilanit specials
Kind, further preferred PEG-30 rilanit specials, Cremophor RH40 (CO40) and PEG-50
One or more in rilanit special.
In step 2, the volume mass ratio of the queen bee larva described in described solubilizer and step 1
It is preferred that 0.3mL/g~0.5mL/g, such as 0.4mL/g.
In step 3, described in the volume sum and step 1 of the liquor B that described water is obtained with step 2
Queen bee larva volume mass ratio preferred 0.8mL/g~1.2mL/g, such as 1mL/g.
In step 3, described sterilizing can be the conventional method of the generic operation in this area, and described goes out
Preferably 80 DEG C~90 DEG C of the temperature of bacterium, further preferred 80 DEG C~82 DEG C, the time of described sterilizing is excellent
Select 20 minutes~1 hour, such as 30 minutes.
Present invention also offers queen bee larva extract I made from above-mentioned preparation method.
Present invention also offers application of the described queen bee larva extract I as cosmetic material.
Present invention also offers a kind of preparation method of queen bee larva extract II, it comprises the following steps：
In water, under conditions of vitamin c (Vc) is present, by the preparation method of queen bee larva extract I
The filter residue being filtrated to get in step 1 is digested, filtered with protease, being dried, and obtains queen bee larva
Extract II；Protease of the described protease for enzymolysis optimal pH 2.0~5.0.
In the preparation method of described queen bee larva extract II, described water is carried with queen bee larva
Take the preferred 3mL/g of volume mass ratio of queen bee larva described in thing I preparation method step 1~
5mL/g, such as 4mL/g.
In the preparation method of described queen bee larva extract II, described enzymolysis optimal pH exists
2.0~5.0 preferred pepsin of protease and/or acid protease.
In the preparation method of described queen bee larva extract II, described protease and queen bee nit
The mass values preferably 0.003 of queen bee larva described in powder extracts I preparation method step 1~
0.01, further preferred 0.004~0.006.
In the preparation method of described queen bee larva extract II, described vitamin c and queen bee children
The mass values preferably 0.002 of queen bee larva described in worm powder extracts I preparation method step 1~
0.006, further preferred 0.004~0.006, such as 0.005.
In the preparation method of described queen bee larva extract II, described enzymolysis can be this area
In the generic operation ordinary temperature, preferably 40 DEG C~60 DEG C of the temperature of described enzymolysis, such as 50 DEG C,
Preferably 3 hours~8 hours, such as 4 hours~6 hours time of described enzymolysis.
In the preparation method of described queen bee larva extract II, described drying can be this area
In the generic operation conventional method, preferably low temperature spray drying or vacuum freeze drying.Described low-temp. spraying
Preferably 100 DEG C~120 DEG C, such as 110 DEG C of the EAT that mist is dried；Described low temperature spray drying
Preferably 50 DEG C~70 DEG C, such as 60 DEG C of leaving air temp.The temperature of described vacuum freeze drying is preferred
- 10 DEG C~-50 DEG C；Preferred 1.3pa~the 13pa of vacuum；Preferably 5 hours time~8 hours, such as 6
Hour.
Present invention also offers queen bee larva extract II made from above-mentioned preparation method.
Food or health product raw material are used as present invention also offers described queen bee larva extract II
Using.
Present invention also offers a kind of queen bee larva extract II I preparation method, it includes following step
Suddenly：The filter residue being filtrated to get in the preparation method of queen bee larva extract II vacuum drying is obtained into queen bee
Larva powder extract II I.
In queen bee larva extract II I preparation method, described vacuum drying can be this area
In the generic operation conventional method, described vacuum drying pressure preferably -0.08MPa~-0.1Mpa；
Preferably 50 DEG C~70 DEG C, such as 60 DEG C of described vacuum drying temperature；When described vacuum drying
Between preferably 6 hours~8 hours.
Present invention also offers queen bee larva extract II I made from above-mentioned preparation method.
Present invention also offers described queen bee larva extract II I answering as animal feed additive
With.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can be combined, and produce this
Invent each preferred embodiments.
Agents useful for same and raw material of the present invention are commercially available.
The present invention positive effect be：
(1) present invention provides preparation technology for many applications of queen bee larva, and the technique can
Three kinds of queen bee nit powder extracts are prepared simultaneously, cosmetics, health care of food product is respectively applied to and supports
Feedstuff industry is grown, the development to promoting apiculture is turned waste into wealth, benefits people, developing queen bee larva
There are highly important economic worth and social value in this high-protein nutrient source.
(2) present invention is prepared in gained queen bee larva alcohol extracting thing containing abundant liposoluble vitamin,
Such as vitamin A. D. E.It can be used as both effectiveness cosmetic material；VA, which has, improves skin
Elasticity, stimulates skin collagen to be formed, the effects such as making epidermal cornified normal.VE have it is anti-oxidant,
UV is reduced to skin damage, wound healing is helped, the effects such as inflammation, moisturizing is resisted, contains VE's
Soap base washes shield class product can make skin more soft after.
(3) present invention preparation gained queen bee larva water extract contains abundant amino acid, peptidic substrate
(total amino acid content reaches as high as reaching 48.9g/100g matter, total shared by its Glutamic Acid and aspartic acid
The ratio of amino acid is that 27.2%), can be applied to as functional raw material in food, health products, due to
The extract is water-soluble good, therefore can be processed to diversified food form.Paddy in extract
The ratio of total amino acid shared by propylhomoserin and aspartic acid can substantially increase more than 20% as brain tonic good merchantable brand
Strong memory, improves intellectual work efficiency.
(4) present invention prepares gained queen bee nit powder extracts chitin, can be used as breeding feed, bait
Feed additives, can adjust microbiota metabolic activity in animal intestinal tract, selectively activate, breed beneficial bacterium
Growth, reduction cholesterol and lipids contents, improves lean meat percentage etc., to improve poultry, fowl, aquatic livestock (fish,
Shrimp, shellfish, ginseng) immunity, premunition and growth promotion and other effects very significantly, also with hindering cause of disease
The function of bacteria growing breeding, can promote protein synthesis, cell activation, so as to improve livestock and poultry production performance.
Embodiment
The present invention is further illustrated below by the mode of embodiment, but is not therefore limited the present invention to
Among described scope of embodiments.The experimental method of unreceipted actual conditions in the following example, according to normal
Rule method and condition, or selected according to catalogue.
Embodiment 1：
(1) queen bee nit powder raw material 100g, adds the ethanol water 300ml that volumetric concentration is 90%
(described volumetric concentration refers to that the volume of ethanol accounts for the percentage of ethanol water cumulative volume), 60 DEG C carry
Take 2 hours.Extraction terminates, and is filtered under diminished pressure, collect filtrate, filtrate decompression be concentrated into it is remaining about 40 milliliters,
Solids weight of giving money as a gift is in filtrate：15 grams~20 grams, the solvent of the low polarity of 0.8ml is added in filtrate
Pentanediol and 30ml solubilizer PEG-30 rilanit specials, are well mixed, add pure water quantitatively extremely
100ml, sterilize (sterilising temp：80 DEG C~82 DEG C, sterilization time：30 minutes), you can obtain honeybee
King larva powder extracts I, yield is 100% (dry matter content：16%), it can be used as a kind of cosmetic
Product raw material.
(2) pure water 300ml is added in the filter residue after above-mentioned steps filtering, then adds the acid eggs of 0.4g
White enzyme and 0.4g vitamin cs (Vc), carry out enzymolysis 3 hours at 50 DEG C；Enzymolysis terminates, and is filtered under diminished pressure,
Filtrate carries out vacuum freeze drying 6 hours (- 30 DEG C, 5.0Pa), you can obtain queen bee nit powder extracts
II, yield is 62%.After testing, total amino acid content is 45.1g/100g, its Glutamic Acid in product
Ratio with total amino acid shared by aspartic acid is 25.2%；In addition also containing water soluble vitamin VC,
VB2、VB6、VB12, nicotinic acid, biotin, folic acid and pantothenic acid.It can be used as raw-food material.
(3) above-mentioned filtered filtration residue, 8 hours (pressure -0.08MPa) of direct 60 DEG C of vacuum drying, is obtained
To queen bee nit powder extracts III, yield is 19%, mainly chitosan kind material, after testing chitin
Mass percentage content be 87%, it can be used as a kind of animal feed additive.
Embodiment 2：
(1) queen bee nit powder raw material 100g, adds the ethanol water 500ml that volumetric concentration is 90%
(described volumetric concentration refers to that the volume of ethanol accounts for the percentage of ethanol water cumulative volume), 40 DEG C carry
Take 3 hours.Extraction terminates, and is filtered under diminished pressure, collect filtrate, filtrate decompression be concentrated into it is remaining about 40 milliliters,
Solids weight of giving money as a gift is in filtrate：15 grams~20 grams, the solvent of the low polarity of 1.2ml is added in filtrate
Propane diols and 50ml solubilizer Cremophor RH40s, are well mixed, and add pure water and quantify to 110ml,
Sterilize (sterilising temp：80 DEG C~82 DEG C, sterilization time：30 minutes), you can obtain queen bee larva
Extract I, yield is 110% (dry matter content：18%).It can be used as a kind of cosmetic material.
(2) pure water 500ml is added in the filter residue after above-mentioned steps filtering, then adds the acid eggs of 0.6g
White enzyme and 0.5g vitamin cs (Vc), carry out enzymolysis 8 hours at 60 DEG C；Enzymolysis terminates, and is filtered under diminished pressure,
Filtrate carries out vacuum freeze drying 8 hours (- 50 DEG C, 13Pa), you can obtain queen bee nit powder extracts
II, yield is 65%, after testing, and total amino acid content is 42.6g/100g, its Glutamic Acid in product
Ratio with total amino acid shared by aspartic acid is 24.8%；In addition also containing water soluble vitamin VC,
VB2、VB6、VB12, nicotinic acid, biotin, folic acid and pantothenic acid.It can be used as raw-food material.
(3) above-mentioned filtered filtration residue, 6 hours (pressure -0.1MPa) of direct 50 DEG C of vacuum drying, is obtained
To queen bee nit powder extracts III, yield is 15%, mainly chitosan kind material, after testing chitin
Mass percentage content be 90%, it can be used as a kind of animal feed additive.
Embodiment 3：
(1) queen bee nit powder raw material 100g, adds the ethanol water 400ml that volumetric concentration is 90%
(described volumetric concentration refers to that the volume of ethanol accounts for the percentage of ethanol water cumulative volume), 50 DEG C carry
Take 3 hours.Extraction terminates, and is filtered under diminished pressure, collect filtrate, filtrate decompression be concentrated into it is remaining about 40 milliliters,
Solids weight of giving money as a gift is in filtrate：15 grams~20 grams, the solvent of the low polarity of 1.0ml is added in filtrate
Butanediol and 40ml solubilizer PEG-50 rilanit specials, are well mixed, and add pure water and quantify to 90ml,
Sterilize (sterilising temp：80 DEG C~82 DEG C, sterilization time：30 minutes), you can obtain queen bee larva
Extract I, yield is 90% (dry matter content：18%) it can be used as a kind of cosmetic material.
(2) pure water 500ml is added in the filter residue after above-mentioned steps filtering, then adds the acid eggs of 0.6g
White enzyme and 0.5g vitamin cs (Vc), carry out enzymolysis 8 hours at 60 DEG C；Enzymolysis terminates, and is filtered under diminished pressure,
Filtrate carries out vacuum freeze drying 5 hours (- 10 DEG C, 1.3Pa), you can obtain queen bee nit powder extracts
II, yield is 62%, after testing, and total amino acid content is 48.9g/100g, its Glutamic Acid in product
Ratio with total amino acid shared by aspartic acid is 27.2%；In addition also containing water soluble vitamin VC,
VB2、VB6、VB12, nicotinic acid, biotin, folic acid and pantothenic acid.It can be used as raw-food material.
(3) above-mentioned filtered filtration residue, 6 hours (pressure -0.09MPa) of direct 50 DEG C of vacuum drying, is obtained
To queen bee nit powder extracts III, yield is 21%, mainly chitosan kind material, after testing chitin
Mass percentage content be 85%, it can be used as a kind of animal feed additive.
Claims (12)
1. a kind of preparation method of queen bee larva extract I, it is characterised in that it comprises the following steps：
Step 1：Queen bee larva, adds volumetric concentration and is extracted for 90%~95% ethanol water,
Filtering, obtains filtrate A, described volumetric concentration refers to that the volume of ethanol accounts for ethanol water cumulative volume
Percentage；
Step 2：Mix, obtain with low polar solvent and solubilizer after the filtrate A concentrations that step 1 is obtained
To mixed liquid B, described low polar solvent is glycolic solvents；
Step 3：The mixed liquid B that step 2 is obtained mixes with water, sterilized, and obtains queen bee larva and carries
Take thing I.
2. the preparation method of queen bee larva extract I as claimed in claim 1, it is characterised in that：
In step 1, described queen bee larva is more than 50% for protein, and ash content is less than 5% queen bee
Larva powder；
And/or,
In step 1, described volumetric concentration is that 90%~95% ethanol water and described queen bee are young
The volume mass ratio of worm powder is 3mL/g~5mL/g；
And/or,
In step 1, the temperature of described extraction is 40 DEG C~60 DEG C；
And/or,
In step 1, the temperature of described extraction is 2 hours~3 hours；
And/or,
In step 1, described being filtered into is filtered under diminished pressure；
And/or,
In step 2, volume and the volume mass of described queen bee larva after described filtrate A concentrations
Preferred 0.3mL/g~the 0.6mL/g of ratio；
And/or,
In step 2, described glycolic solvents are one kind or many in propane diols, butanediol and pentanediol
Kind；
And/or,
In step 2, described glycolic solvents and the volume mass of the queen bee larva described in step 1
Ratio is 0.008mL/g~0.012mL/g；
And/or,
In step 2, described solubilizer is Cremophor RH40 class solubilizer；
And/or,
In step 2, the volume mass ratio of the queen bee larva described in described solubilizer and step 1
For 0.3mL/g~0.5mL/g；
And/or,
In step 3, described in the volume sum and step 1 of the liquor B that described water is obtained with step 2
Queen bee larva volume mass ratio be 0.8mL/g~1.2mL/g；
And/or,
In step 3, preferably 80 DEG C~90 DEG C of the temperature of described sterilizing.
3. the preparation method of queen bee larva extract I as claimed in claim 2, it is characterised in that：
In step 1, described volumetric concentration is that 90%~95% ethanol water and described queen bee are young
The volume mass ratio of worm powder is 4mL/g；
And/or,
In step 1, the temperature of described extraction is 50 DEG C；
And/or,
In step 2, volume and the volume mass of described queen bee larva after described filtrate A concentrations
The preferred 0.4mL/g of ratio；
And/or,
In step 2, described glycolic solvents and the volume mass of the queen bee larva described in step 1
Ratio is 0.01mL/g；
And/or,
In step 2, described Cremophor RH40 class solubilizer is PEG-30 rilanit specials,
PEG-35 rilanit specials, Cremophor RH40, PEG-45 rilanit specials and PEG-50 hydrogen
Change the one or more in castor oil；
And/or,
In step 2, the volume mass ratio of the queen bee larva described in described solubilizer and step 1
For 0.4mL/g；
And/or,
In step 3, described in the volume sum and step 1 of the liquor B that described water is obtained with step 2
Queen bee larva volume mass ratio be 1mL/g；
And/or,
In step 3, preferably 80 DEG C~82 DEG C of the temperature of described sterilizing.
4. the preparation method of the queen bee larva extract I as described in any one of claims 1 to 3 is made
Queen bee larva extract I.
5. a kind of preparation method of queen bee larva extract II, it is characterised in that it comprises the following steps：
In water, under conditions of vitamin c is present, the queen bee larva described in claim 1 or 2 is extracted
The filter residue being filtrated to get in thing I preparation method step 1 is digested, filtered with protease, being dried,
Obtain queen bee larva extract II；Described protease is enzymolysis optimal pH 2.0~5.0
Protease.
6. the preparation method of queen bee larva extract II as claimed in claim 5, it is characterised in that：
In the preparation method of described queen bee larva extract II, described water is carried with queen bee larva
Take the volume mass ratio of queen bee larva described in thing I preparation method step 1 for 3mL/g~
5mL/g；
And/or,
In the preparation method of described queen bee larva extract II, described enzymolysis optimal pH exists
2.0~5.0 protease is pepsin and/or acid protease；
And/or,
In the preparation method of described queen bee larva extract II, described protease and queen bee nit
The mass values of queen bee larva described in powder extracts I preparation method step 1 are 0.003~0.01；
And/or,
In the preparation method of described queen bee larva extract II, described vitamin c and queen bee children
The mass values of queen bee larva described in worm powder extracts I preparation method step 1 be 0.002~
0.006；
And/or,
In the preparation method of described queen bee larva extract II, the temperature of described enzymolysis is
40 DEG C~60 DEG C；
And/or,
In the preparation method of described queen bee larva extract II, the time of described enzymolysis is small for 3
When~8 hours；
And/or,
In the preparation method of described queen bee larva extract II, described drying is dry for cold nebulization
Dry or vacuum freeze drying.
7. the preparation method of queen bee larva extract II as claimed in claim 6, it is characterised in that：
In the preparation method of described queen bee larva extract II, described water is carried with queen bee larva
The volume mass ratio for taking the queen bee larva described in thing I preparation method step 1 is 4mL/g；
And/or,
In the preparation method of described queen bee larva extract II, described protease and queen bee nit
The mass values of queen bee larva described in powder extracts I preparation method step 1 be 0.004~
0.006；
And/or,
In the preparation method of described queen bee larva extract II, described vitamin c and queen bee children
The mass values of queen bee larva described in worm powder extracts I preparation method step 1 be 0.004~
0.006；
And/or,
In the preparation method of described queen bee larva extract II, the time of described enzymolysis is small for 4
When~6 hours；
And/or,
In the preparation method of described queen bee larva extract II, described low temperature spray drying enters
Air temperature is 100 DEG C~120 DEG C；
And/or,
In the preparation method of described queen bee larva extract II, described low temperature spray drying goes out
Air temperature is 50 DEG C~70 DEG C；
And/or,
In the preparation method of described queen bee larva extract II, the temperature of described vacuum freeze drying
Spend for -10 DEG C~-50 DEG C；
And/or,
In the preparation method of described queen bee larva extract II, the pressure of described vacuum freeze drying
It is 1.3pa~13pa by force；
And/or,
In the preparation method of described queen bee larva extract II, described vacuum freeze drying when
Between be 5 hours~8 hours.
8. the preparation method of the queen bee larva extract II as described in any one of claim 5~7 is made
Queen bee larva extract II.
9. a kind of queen bee larva extract II I preparation method, it is characterised in that it comprises the following steps：
It will be filtrated to get in the preparation method of queen bee larva extract II described in any one of claim 5~7
Filter residue vacuum drying obtain queen bee larva extract II I.
10. queen bee larva extract II I as claimed in claim 9 preparation method, its feature exists
In：
In queen bee larva extract II I preparation method, described vacuum drying pressure is
- 0.08MPa~-0.1Mpa；
And/or,
In queen bee larva extract II I preparation method, described vacuum drying temperature is
50 DEG C~70 DEG C；
And/or,
In queen bee larva extract II I preparation method, the described vacuum drying time is small for 6
When~8 hours.
11. made from the preparation method of the queen bee larva extract II I as described in claim 9 or 10
Queen bee larva extract II I.
12. queen bee larva extract I as claimed in claim 4 is used as the application of cosmetic material；
And/or,
Queen bee larva extract II as claimed in claim 8 as food or health product raw material application；
And/or,
Queen bee larva extract II I as claimed in claim 11 as animal feed additive application.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300618A (en) * | 2018-04-17 | 2018-07-20 | 北京农科亿健蜂产品研究院 | A kind of nti-freckle perfumed soap and preparation method thereof |
| CN108753885A (en) * | 2018-06-11 | 2018-11-06 | 华中农业大学 | The preparation method and queen bee nit glucosidase suppressor activity peptide of a kind of queen bee nit glucosidase suppressor activity peptide and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090977A (en) * | 1993-02-15 | 1994-08-24 | 邹莲芳 | Bee pupa series nutriment and preparation method thereof |
| CN102696767A (en) * | 2012-06-15 | 2012-10-03 | 江南大学 | Deep processing method for preparing low-fat walnut milk, active peptide and protein powder by comprehensively utilizing walnut kernels |
| CN104546980A (en) * | 2013-10-21 | 2015-04-29 | 中国科学院大连化学物理研究所 | Bee pollen extract as well as preparation and application thereof |
-
2016
- 2016-01-05 CN CN201610005286.1A patent/CN106937731A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090977A (en) * | 1993-02-15 | 1994-08-24 | 邹莲芳 | Bee pupa series nutriment and preparation method thereof |
| CN102696767A (en) * | 2012-06-15 | 2012-10-03 | 江南大学 | Deep processing method for preparing low-fat walnut milk, active peptide and protein powder by comprehensively utilizing walnut kernels |
| CN104546980A (en) * | 2013-10-21 | 2015-04-29 | 中国科学院大连化学物理研究所 | Bee pollen extract as well as preparation and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300618A (en) * | 2018-04-17 | 2018-07-20 | 北京农科亿健蜂产品研究院 | A kind of nti-freckle perfumed soap and preparation method thereof |
| CN108753885A (en) * | 2018-06-11 | 2018-11-06 | 华中农业大学 | The preparation method and queen bee nit glucosidase suppressor activity peptide of a kind of queen bee nit glucosidase suppressor activity peptide and application |
| CN108753885B (en) * | 2018-06-11 | 2019-05-07 | 华中农业大学 | The preparation method and queen bee nit glucosidase suppressor activity peptide of a kind of queen bee nit glucosidase suppressor activity peptide and application |
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