CN1090977A - Bee pupa series nutriment and preparation method thereof - Google Patents
Bee pupa series nutriment and preparation method thereof Download PDFInfo
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- CN1090977A CN1090977A CN93101793A CN93101793A CN1090977A CN 1090977 A CN1090977 A CN 1090977A CN 93101793 A CN93101793 A CN 93101793A CN 93101793 A CN93101793 A CN 93101793A CN 1090977 A CN1090977 A CN 1090977A
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Abstract
Bee pupa series nutriment and preparation method thereof.The invention belongs to the nutraceutical and the processing method thereof of a kind of food and processing method thereof, particularly honeybee.The present invention is a raw material with bee pupa and/or larva, uses the mode that enzymolysis and lactic fermentation combine, and fully extracts the multiple nutrients material in bee pupa and the larva, adopts different technology then, makes nutrition oral administration, nutritional emulsions and particle instant.Process of the present invention is science comparatively rationally, can propose than multi-nutrient, and the product edible safety is reliable, and mouthfeel is better, can be used as the children of scarce zinc and old man's natural zinc supplementation agent and health-care tonic product.
Description
The present invention relates to the nutraceutical and the processing method thereof of food and processing method thereof, particularly a kind of honeybee.
The delicious food that people like when bee pupa and larva of bee, the effect that have nutritious tonifying, improves the health.China is among the people generally to be that frying back is edible, and the developed country of raising bees in the world is made into can or adds in the food and sell as senior nutrition, and these eating methods are all lower to the nutrition utilization rate of honeybee.In order to increase operation rate, obtain better nutriment, Chinese patent literature CN1040910A discloses a kind of method with alcohol extract bee pupa and larva of bee nutriment, but because the body wall of honeybee is the compound that chitin and protein constitute, can only propose less nutriment; Another patent documentation CN1045276A goes a step further, it will be used for extracting bee pupa and larva of bee nutrition with the enzyme process method of separating protein in pharmacy and the grocery trade, more nutriment can be suggested, but this method recovery rate is still waiting to improve, and because enzymolysis time is too short, macromolecular substances is decomposed thorough inadequately, also may contain in the extract and have antigenic macromolecular substances, cause reaction in various degree after the allergic constitution person is edible sometimes.The product mouthfeel that obtains according to this method is single in addition, moreover the sterilization measure that there is no need in enzymolysis process of this method, is easy to be polluted by harmful microorganisms such as bacteriums, causes taking dangerous.
The purpose of this invention is to provide a kind of extraction bee pupa nutrition, and then be prepared into the method for bee pupa series nutriment and the serial nutrious product made from this method, and it is higher that this method has recovery rate, and technology is comparatively scientific and reasonable, finished product is taken safety, and taste is characteristics preferably.
Implementation method of the present invention is: adopt enzymolysis and lactic fermentation two methods to carry out the nutrition extraction to bee pupa and/or larva of bee, also need before the enzymolysis enzymolysis material heating disinfection and water hot extraction, its pH value is transferred to the upper limit of used enzyme optimum PH, enzymolysis time is 6～48 hours, need after the enzymolysis be heated to 85～100 ℃, be incubated 10～30 minutes go out enzyme with disinfect.In above-mentioned measure, should do lactic fermentation behind the enzymolysis handles, so that more nutrition is suggested, and big molecule such as protein thoroughly is decomposed into small-molecule substance, the antigenicity that this both can eliminate big molecule anaphylactogen material makes edible safer reliably, can be easier to be absorption of human body again, because the interaction energy of lactic acid bacteria produces multiple organic acid, this can make product special flavour improve in addition; To sterilization of enzymolysis material heating and water hot extraction, can kill harmful bacteria microorganism wherein before the enzymolysis, make and take safety; For making protein fully hydrolysis in enzymolysis, 6～48 hours these longer enzymolysis times have been adopted; Therefore for avoiding still retentive activity of protease, cause microbial contamination behind the enzymolysis, should do 85～100 ℃, the enzyme and disinfecting of going out of 10～30 minutes time.
The concrete processing step that extracts nutrition in bee pupa and/or the larva of bee is:
(1) flowing water soaks the rinsing raw material, weighs wherein impurity to remove,
(2) add drinking water, smudge cells is made homogenate, so that nutriment separates out,
(3) under 75～100 ℃ of conditions, make homogenate insulation 1～60 minute, by the heating disinfection sterilization, extract nutriment on the other hand on the one hand,
(4) regulate pH value to the used protease optimum PH upper limit, thereby a large amount of amino acid hydrolysis when guaranteeing enzymolysis are unlikely to make pH value to drop to below the optimum PH scope, have influence on the extraction of nutritional labeling,
(5) be cooled to 30～55 ℃, with protease hydrolytic protein and polypeptide wherein, enzymolysis time 6～48 hours, temperature is 20～75 ℃,
(6) at 85～100 ℃, heat go out enzyme and sterilization under 10～35 minutes conditions,
(7) be cooled to 30～50 ℃, add lactobacillus solution or leavening and carry out lactic fermentation,
(8) heat-killed lactic acid bacteria stops sweat,
(9) separation obtains nutriment extract and residue.
In above-mentioned extraction nutrition technology, should grasp following technological parameter, to obtain result preferably: when the homogenate of fragmentation system, water is 2～7: 1 with the ratio of the weight of honeybee, the pH value of homogenate transfers to 3～9, the protease that adopts in the enzymolysis is except that selecting for use the papain, main also available trypsase, edible protein enzymes such as compound protease, the consumption of enzyme is 1000～9000 units/gram bee pupas, the bacterial classification of selecting for use during lactic fermentation is streptococcus thermophilus (Streptococa thermophilus) and lactobacillus bulgaricus (Lac-tobacillus bulgaricus), 34～50 ℃ of fermentation temperatures, 10～20 hours time, fermentating liquid PH value is 4.0～4.8 when stopping fermentation.
Be to prepare nutrition oral administration with the above-mentioned process of the present invention below, several embodiment of nutritional emulsions and nutritional granular instant are further carefully to state the present invention.
Embodiment 1:
Get the bee pupa and the larva of salt marsh and in flowing water, soaked 40 minutes, in 60 ℃ of water, be incubated 10～20 minutes, to remove salt wherein.Pull and add the drinking water of 4 times of its weight in wet bases out, in homogenizer, be broken into homogenate.Homogenate is incubated 40 minutes down at 80 ℃, and the pH value of this moment is about between 5.8～6.5, and color is creamy white, the Ca(OH with 20%) aqueous ph value to 7.8.Add Ca(OH) should slowly splash into during the aqueous solution, stir while dripping.When being cooled to 40 ℃, add the ratio of compound protease 4000 units in every gram bee pupa, enzyme-added in homogenate, 40～45 ℃ of following enzymolysis 8 hours, carry out press filtration then, add 3% lactose, filtrate is sterilized under 95～100 ℃ and the enzyme 10 minutes of going out.To be cooled during to 42 ℃, add the seed liquor (the two ratio is 1: 1) that contains streptococcus thermophilus and lactobacillus bulgaricus, 38～40 ℃ of condition bottom fermentations 12～18 hours, treat that fermentating liquid PH value reduced at 4.3 o'clock, the heat-killed lactic acid bacteria stops fermentation.Separate through press filtration, obtain nutriment extract and the residue of bee pupa and larva.Nutriment extract and water-soluble stabilizing agent and food emulsifier are mixed, stabilizing agent can be selected anionic polymer carboxymethyl fine plain (CMC) for use, food emulsifier can be selected Tween 80 (Toween80), sorbester p38 (Span65) and sucrose ester etc. for use, add raw materials such as honey, sucrose again and transfer taste and transfer volume, make homogeneous with high pressure homogenizer again and handle.Then with the emulsion that obtains 85～90 ℃ of following heating disinfections 20 minutes, filling and sealing promptly obtains the bee pupa nutritional emulsions in sterile chamber.
Embodiment 2:
With fresh bee pupa slightly rinse-added after, add the heavy drinking water of 3 times of bee pupas, make homogenate with the colloid mill smudge cells, under 95 ℃ of conditions, add extraction heat then, 20 minutes time.Behind 20%NaOH liquid accent pH value to 8.2, speed is cooled to 40～45 ℃, in the ratio of every gram bee pupa 4000 unit enzymes, adds the papain of dissolving, is warming up to 65 ℃ of enzymolysis again 8 hours, filters with filter press then.Filter and to make the animal feed addictive after resulting residue dries.In filtrate, add 2.5% lactose, and be heated to 95 ℃ of enzyme and sterilizations 15 minutes of going out, to be cooled during to 40 ℃, add the seed liquor that contains streptococcus thermophilus and lactobacillus bulgaricus.38 ℃ of bottom fermentations 14 hours, wait fermentating liquid PH value to reduce at 4.1 o'clock, be heated to 100 ℃, be incubated 10 minutes, kill lactic acid bacteria and stop fermentation.When being cooled to 50 ℃, add stabilizing agent carboxymethyl cellulose (CMC) and food emulsifier Tween 80 (Toween80), make homogeneous with high pressure homogenizer then and handle.Then be concentrated into the paste that soaks of proportion 1.0-1.4 with vacuum concentration pot, mixed with excipients is even adding, and edible ethanol damping degree.Excipient can adopt cane sugar powder, milk powder and soluble starch; concentrated extract and its percentage are 20% and 80%; edible ethanol can be with 70%; carry out wet granulation with granulator then; wet granular is after 55-60 ℃ of following drying; become the particle of 0.1-5 millimeter again with the whole grain of granulator, be packaged into the bee pupa nutritional granular instant of one bag of 30 gram with the automated packing packing machine.
Embodiment 3:
Ethanol and impurity are removed in the bee pupa rinsing of alcohol immersion, the water mill that places colloid mill to add 3 times becomes homogenate, under 90-95 ℃, add nutriment in the extraction heat homogenate, time is 20 minutes, transfers pH value to 8.3 with 20%NaOH liquid then, during liquid temperature drop to 45 ℃, add compound protease, enzyme dosage is 4000 units/gram bee pupas, under 50-55 ℃ of condition, and enzymolysis 14 hours.In enzymolysis liquid, add 3% lactose behind the enzymolysis, and under 95-100 ℃, sterilize and the enzyme 15 minutes of going out, treat that the temperature cooling is in the time of 42 ℃, add the leavening or the seed liquor (ratio of two bacterial classifications is 1: 1) that contain streptococcus thermophilus and lactobacillus bulgaricus, then at 38-40 ℃ of bottom fermentation 12-18 hour, when dropping to 4.2-4.3 Deng fermentating liquid PH value, the heat-killed lactic acid bacteria stops fermentation.Separate through press filtration, obtain bee pupa nutriment extract and residue.After drying, residue can make the animal feed addictive.The nutriment extract extracts its supernatant after leaving standstill, through transferring taste, transferring volume, homogeneous is handled, and filters, and behind filling and sealing machine can envelope bottle, carries out autoclaving, makes the bee pupa nutrition oral administration of a kind of every bottle of 10ml after the packing.And the precipitation of extract partly can add emulsifier tween 80(Toween80) and sucrose ester after; making homogeneous with homogenizer handles; the paste of decompression simmer down to proportion 1.1 in vacuum concentration pan then; to excipient such as the cane sugar powder that wherein adds an amount of 80-100 of mistake mesh sieve, soluble starches; mix, carry out vacuum drying (55-60 ℃, the 650-740 millimetres of mercury) after the sabot; behind broken granulator granulation and whole grain, pack and be a kind of bee pupa nutritional granular quick-dissolving agent.
Implementation and embodiment from the invention described above, the present invention can decomposite the protein in bee pupa and the larva preferably, processing step and parameters selection are rationally and science, can extract more ground nutriment, and the big molecular breakdown that can will contain the anaphylactogen material is little molecule, eliminate its antigenicity, it is safer that product is taken, and local flavor also increases.The bee pupa series nutriment that the present invention is prepared into shows through clinical and zoopery, to lacking the zinc children appetite that improves significantly, promote the effect of growth, grow when the zinc rat also has obvious urging to lacking, whet the appetite, promote hemoglobin content is increased, promote the many-sided effect such as growth of vitals and immune organ, therefore can be used as natural zinc supplementation health-care tonic product.The nutriment extract of bee pupa can also be made the additive and the hardening agent of other nutraceutical, and the additive of cosmetics.
Claims (8)
1, a kind of bee pupa series nutriment, it comprises nutrition oral administration, nutritional emulsions and nutritional granular instant, it is characterized in that contained nutrition is bee pupa and/or the extract of larva of bee after enzymolysis and lactic fermentation processing.
2, a kind of preparation method of bee pupa series nutriment, it is characterized in that the extraction of the nutrition of bee pupa and/or larva of bee has been comprised enzymolysis and two key steps of lactic fermentation, will be before enzymolysis processing to enzymolysis material heating disinfection and water hot extraction, transfer the upper limit of pH value to the suitableeest pH value of used enzyme, enzymolysis 6～48 hours, be heated to 85～100 ℃ after the enzymolysis processing again, be incubated 10～30 minutes the enzyme and disinfecting of going out.
3, by the preparation method of the described bee pupa series nutriment of claim 2, the processing step that it is characterized in that extracting the nutrition of honeybee is:
(1) flowing water soaks rinsing,
(2) add drinking water, smudge cells is made homogenate,
(3) heating disinfection and extraction, 75～100 ℃ of temperature, 1～60 minute time,
(4) regulate the upper limit of pH value to the optimum PH of protease,
(5) be cooled to 30～55 ℃, with proteolytic enzyme protolysate matter and polypeptide, 6～48 hours time, 20～75 ℃ of temperature,
(6) heat go out enzyme and disinfection, 85～100 ℃ of temperature, 10～30 minutes time,
(7) be cooled to 34～50 ℃, insert lactobacillus solution or leavening and carry out lactic fermentation,
(8) after the fermentation, the heat inactivation lactic acid bacteria,
(9) separation obtains nutriment extract and residue.
4, preparation method by the described bee pupa series nutriment of claim 3, it is characterized in that when bee pupa and/or the homogenate of the broken system of larva of bee, the weight ratio of water and honeybee is 2～7: 1, the pH value of solution is 3～9, protease consumption during enzymolysis is 1000～9000 units/gram bee pupas, the bacterial classification of selecting for use during lactic fermentation is streptococcus thermophilus (Streptococa thermophilus) and lactobacillus bulgaricus (Lac-tobacillus bulgaricus), fermentation temperature is 34～50 ℃, time is 10～20 hours, and fermentating liquid PH value was 4.0～4.8 when fermentation stopped.
5, by the preparation method of the described bee pupa series nutriment of claim 4, it is characterized in that the preparation technology of nutritional granular instant is:
(1) the nutriment extract after enzymolysis and lactic fermentation are handled mixes with water-soluble stabilizing agent and food emulsifier,
(2) spare matter and handle,
(3) being concentrated into proportion is 1.0～1.4 the paste that soaks,
(4) mix behind the adding excipient, with edible ethanol damping degree,
(5) carry out wet granulation with granulator,
It is (6) dry under 40～60 ℃ of conditions,
(7) broken, whole grain is made 0.1～5 millimeter particle.
6, by the preparation method of the described bee pupa series nutriment of claim 4, it is characterized in that the preparation technology of nutrition oral administration is:
(1) extract after enzymolysis and the lactic fermentation processing is left standstill, gets its supernatant, transfer taste, transfer volume,
(2) homogeneous is handled,
(3) filter,
(4) embedding,
(5) sterilization obtains nutrition oral administration.
7, by the preparation method of the described bee pupa series nutriment of claim 4, it is characterized in that the preparation technology of nutritional emulsions is:
(1) extract after enzymolysis and the lactic fermentation is mixed with water-soluble stabilizing agent and food emulsifier,
(2) transfer taste, transfer volume,
(3) homogeneous is handled,
(4) heating disinfection sterilization,
(5) embedding is in sterile chamber.
8, press the preparation method of the described bee pupa series nutriment of claim 3, it is characterized in that separating obtained residue can make the animal feed addictive, separate the additive that the nutrition extract that obtains can be used as the additive of zinc supplementation health-care tonic product, nutraceutical and hardening agent, cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93101793A CN1090977A (en) | 1993-02-15 | 1993-02-15 | Bee pupa series nutriment and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93101793A CN1090977A (en) | 1993-02-15 | 1993-02-15 | Bee pupa series nutriment and preparation method thereof |
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| Publication Number | Publication Date |
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| CN1090977A true CN1090977A (en) | 1994-08-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN93101793A Pending CN1090977A (en) | 1993-02-15 | 1993-02-15 | Bee pupa series nutriment and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010030975A (en) * | 2008-06-23 | 2010-02-12 | Api Co Ltd | Bee larva solubilized product, preparation method thereof, and pharmaceutical product, cosmetic, or food and drink containing the bee larva solubilized product |
| CN102972727A (en) * | 2012-12-25 | 2013-03-20 | 广东省农业科学院蚕业与农产品加工研究所 | Insect-derived flavor-enhancing peptide and preparation method thereof |
| CN103340409A (en) * | 2013-07-29 | 2013-10-09 | 谢娟 | Bee pupa and pawpaw cream and processing method thereof |
| CN105876294A (en) * | 2014-11-16 | 2016-08-24 | 李志凌 | Processing and manufacturing method of special dried honeybee pupaes |
| CN106937731A (en) * | 2016-01-05 | 2017-07-11 | 上海辉文生物技术股份有限公司 | Queen bee nit powder extracts and its preparation method and application |
| CN107397221A (en) * | 2017-08-07 | 2017-11-28 | 杨昌团 | A kind of health care bee pupa powder and preparation method thereof |
| CN110960555A (en) * | 2019-12-26 | 2020-04-07 | 普洱学院 | Bee pupa oral liquid with antioxidation effect |
-
1993
- 1993-02-15 CN CN93101793A patent/CN1090977A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010030975A (en) * | 2008-06-23 | 2010-02-12 | Api Co Ltd | Bee larva solubilized product, preparation method thereof, and pharmaceutical product, cosmetic, or food and drink containing the bee larva solubilized product |
| CN102972727A (en) * | 2012-12-25 | 2013-03-20 | 广东省农业科学院蚕业与农产品加工研究所 | Insect-derived flavor-enhancing peptide and preparation method thereof |
| CN102972727B (en) * | 2012-12-25 | 2014-04-09 | 广东省农业科学院蚕业与农产品加工研究所 | Insect-derived flavor-enhancing peptide and preparation method thereof |
| CN103340409A (en) * | 2013-07-29 | 2013-10-09 | 谢娟 | Bee pupa and pawpaw cream and processing method thereof |
| CN105876294A (en) * | 2014-11-16 | 2016-08-24 | 李志凌 | Processing and manufacturing method of special dried honeybee pupaes |
| CN106937731A (en) * | 2016-01-05 | 2017-07-11 | 上海辉文生物技术股份有限公司 | Queen bee nit powder extracts and its preparation method and application |
| CN107397221A (en) * | 2017-08-07 | 2017-11-28 | 杨昌团 | A kind of health care bee pupa powder and preparation method thereof |
| CN110960555A (en) * | 2019-12-26 | 2020-04-07 | 普洱学院 | Bee pupa oral liquid with antioxidation effect |
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