AU2020102282A4

AU2020102282A4 - Immunity-boosting gel candy for children and the method of preparation

Info

Publication number: AU2020102282A4
Application number: AU2020102282A
Authority: AU
Inventors: Hui An; Luodan Xu; Zhenglai Zhou
Original assignee: Unipharm Healthy Manufacturing Co Ltd
Current assignee: Unipharm Healthy Manufacturing Co Ltd
Priority date: 2020-09-16
Filing date: 2020-09-16
Publication date: 2020-11-05
Anticipated expiration: 2028-09-16

Abstract

The present invention explores a gel candy which helps boost children's immunity. The immunity-boosting gel candy is composed of both a shell and nutrient content. The content includes the following components in part: 230 to 250 parts of DHA algal oil, 8~12 parts of N-acetylneuraminic acid, 120~140 parts of walnut oil, 10~20 parts of lemon oil, 5~10 parts of mono-and diglycerides of fatty acids, 0.2~0.4 parts of steviol glycosides. The present invention adopts highly safe vegetable oils as a raw material to prepare a gel candy which improves immunity, has no toxic side effects, is extremely safe, does not cause allergic reactions, can effectively promote a children's nutritional balance and ultimately improves children's immunity as a whole. Additionally, the method of preparation for the immunity-boosting gel candy is extremely simple to operate.

Description

DESCRIPTION TITLE

Immunity-boosting gel candy for children and the method of preparation

FIELD OF THE INVENTION

The invention is related to the field of gel candy more s

pecifically, a gel candy which helps boost children's immunity and the method of preparation.

BACKGROUNGD OF THE INVENTION

Candy is both an indispensable and popular food for many individuals in their daily lives. Not

to mention there are many varieties of candy such as fruit candy, toffee and hard candy. As

children tend to have a love for candy and are the usually main consumers, the development

of an immunity-boosting gel candy for children will have a significant social impact and

exceptional market value.

Gel candy is a soft capsule product filled with a liquid to form the capsule and closed with an

exterior shell. They can be consumed in many ways, as a simple container (not to be digested),

swallowed whole or crushed. Gel candy is currently a very popular dietary supplement, its

formation of the gel depending hugely on the hydration levels. Therefore, all gels used in

candy must be hydrophilic colloids. When the hydrophilic groups (-OH, -COOH, etc.) of the

colloidal molecules absorb and maintain a certain amount of water forming hydration, water

acts as a solvent as molecules cover it to form a hydration layer. At the same time, the

non-hydrophilic groups (-CH3, -CH2, etc.) of the colloidal molecules generate mutual

attraction between molecules. This helps to form single micelles into linear molecules and

gather them into bundles of micelles.

Many products on the market claim to boost or support immunity. However, the concept of

boosting immunity tends to make little sense scientifically. In fact, boosting the number of cells in your body (both immune cells or others) is not necessarily good for your health. For example, athletes who participate in "blood doping" - the pumping of blood into their system to boost the number of blood cells to enhance their performance - many run the risk of having a stroke. Attempting to boost the cells of your immune system is especially complicated as there are so many different varieties of cells in the immune system that respond to many different microbes in various ways. Which cells should you be boosting, and to what number?

So far there is no clear scientific answer. What is known is that the body continually generates

immune cells, it produces many more lymphocytes than it can possibly use. The extra cells

remove themselves through a natural process of cell death called apoptosis. The deaths can

vary and depend on if there was anything they were able to combat or not. Notably, it is

unclear as to how many cells or what the best composition of cells the immune system needs

to function at its optimal level.

For a healthy immune system, well-balanced and regular nourishment is necessary. Scientists

have long recognised that individuals who live in poverty and may be malnourished are much

more vulnerable to infectious diseases. Whether the increased rate of diseases is caused by

malnutrition effects on the immune system is however not certain. There are relatively few

studies on the effects of nutrition on the immune system of humans.

There is some evidence outlining that various micronutrient deficients - For example,

deficiencies of zinc, selenium, iron, copper, folic acid, and vitamins A, B6, C, and E - alter

immune responses in animals (as measured in a test tube). However, the impact of these

immune system changes for the health of the animal is unclear, and the effect of similar

deficiencies on the human immune response has yet to be assessed.

So what can we do? If you suspect your diet is not providing you with all the micronutrients

needed - such as vegetables - taking a daily multivitamin and mineral supplement may

provide health benefits and have beneficial effects on the immune system. With that said,

taking a megadose of one vitamin may not be necessarily more beneficial.

As children do not have a fully developed immune system, they have an increased risk of

influenza infection. Children do not respond to certain vaccines in the same way adults do and

they do not produce polysaccharide antigen until they are about five years old. The immune

system grows and develops as the child does, meaning it is not entirely developed until

puberty, as sex hormones are responsible for a fully mature and developed immune system.

US20020054880 describes peptides that can bind to the end of a-sialic acid (2-6)pGal

and/or a-sialic acid (2-3)pGal- groups, and their use to inhibit the immune response or cell

interaction in mammals used in the method. The immunomodulatory effects of silica acid

binding molecules are not disclosed.

N-acetylneuraminic acid was first discovered in 1936, it is known as the most widely

distributed sialic acid among nature. It is an acylated nine-carbon sugar containing carboxyl

group, also called sugar acid, which is mainly animal cell membrane or secretion. The

glycoprotein, glycolipid or bacterial capsular substance and other constituent sugars. Sialic

acid is a type of neuraminic acid which maternal structure is composted of nine carbon atoms.

It is found in various biological tissues and is a component of complex carbohydrates on the

cell's surface. The most common sialic acid found in most mammalian tissue is

N-acetylneuraminic acid, usually referred to as sialic acid. Wang Bing (School of Molecular

and Microbial Biological Sciences, University of Sydney, New South Whales, Australia) has

published many papers on sialic acid, he is an accredited expert in the field of sialic acid. In

2003, he published an article "The role and Potential of' in the European Journal of Clinical

Nutrition. In the article, "Sialic Acid in Human Nutrition", sialic acid is hailed as an

important nutrient component that may affect the brain development of children and infants

after DHA (Docosahexaenoic acid). This too promoted breastfeeding as an important reason

as it compared the nutritional value of animal milk and human milk. The content of sialic acid

in breast milk is 2.5 times that of animal milk, it can be seen that there is a big gap between

the type and content of sialic acid in each of the milks. However, the existing

N-acetylneuraminic acid antiviral composition of antiviral and immune-improving powder formulations rarely uses N-acetylneuraminic acid. It has mostly been used as a brain nutrition in the past it too tends to be matched with the formula formed by other ingredients which lack scientific and reasonable basis. This does not allow for the full efficacy of the respective ingredients, the dosage form is also single which cannot meet the needs of user groups.

Studies have shown that some compounds that can bind to sialic acid can be used in the

treatment and prevention of diseases, especially diseases with microbial ethology such as

respiratory diseases. Moreover, current studies have shown that molecules which exhibit the

ability to bind with sialic acid can be used to neutralise or present infections caused by

pathogens that utilise the presence of sialic acid on the surface of host cells. For example,

respiratory pathogens, such as viruses belonging to the Orthomyxoviridae or Paramyxoviridae

family, and certain streptococci use receptors containing sialic acid on the cell surface to bind

to and enter specific cell types in various mammalian tissues. Compounds such as proteins

and peptides, which bind to sialic acid moieties and/or molecules containing them (such as

cell surface receptors) can be used to treat and/or prevent diseases caused or contributed to by

pathogens that utilise cells Surface sialic acid receptors serve as a means of binding or

attaching cells.

Without wishing to be bound by theory, sialic acid binding molecules may interfere, inhibit,

prevent and/or block the interaction between the pathogen and the sialic acid receptor on the

cell's surface thereby preventing the pathogen from binding or attaching. It has also been

found that certain sialic acid binding molecules which prevent the interaction with the

pathogen and for example the cell surface receptor containing sialic acid has

immunomodulatory properties. The term "immunomodulation" is used for the observed

effects of various sialic acid binding molecules of the present invention on the host immune

response, including any activation of the immune system and/ or immune system processes,

pathways, and/or any of them. The term "priming" used for an immune response may include

the phenomenon of increasing the susceptibility and/ or response of the immune system to

immunogens, antigens, pathogens, diseases and infections. Again without wishing to be tied to theory, any immunomodulatory properties reared to the sialic acid-binding molecule of the present invention, subjects administered or in contact with the sialic acid-binding molecule can be more able to cope with the occurrence of infection/disease.

Algae can synthesise DHA through self-fermentation. Therefore, with the development of

modem technology, seaweed is grown in large stainless-steel fermentation tanks commonly

used in the pharmaceutical, food and biotechnology industries. In accordance with Good

Manufacturing Practices (GMP), the entire production process is controlled to ensure the

algae is clean, environmentally friendly and free from pollution. This ensures the production

of high-quality DHA which is safe, efficient and pollution-free. Algae has high DHA content

but almost no EPA (Eicosapentaenoic acid). If DHA is extracted from algae, the ratio of DHA

to EPA is 20:1, if it is extracted from fish oil, it is (4-5):1. DHA extracted from algae is

natural, plant-based, has a strong antioxidant capacity, and is low in EPA content. Meanwhile,

DHA extracted from deep-sea fish oil has relatively active properties and is prone to oxidative

denaturation with extremely high EPA content. EPA can have the effect of lowering blood fat

and thinning blood, this means DHA and EPA extracted from deep-sea fish oil is beneficial

for mainly adults and the elderly. DHA extracted from seaweed oil is most useful for children

and infants, it can effectively promote the development of the infant's retina and brain

function. Mothers are recommended to choose algae DHA as the academic community also

agrees that DHA algae oil is more suitable for children and infants. DHA accounts for

roughly 97% of the omega-3 fatty acids in the brain, the human body maintains a normal

function of various tissues and must ensure that there are sufficient and various fatty acids.

However, among various fatty acids linoleic acid o6 and linolenic acid o3 cannot be used by

the human body. As a kind of fatty acid, DHA has a significant effect on memory building,

thinking abilities, and improved intelligence. Population epidemiology studies have found

that people with high levels of DHA in their bodies have stronger psychological endurance

and a higher intellectual development index.

Seaweed oil is the general term for all oils in seaweed. DHA algal oil is usually a slightly

yellow liquid at room temperature and its applications mainly include the production of DHA

algal oil health products and biodiesel. Seaweed oil is rich in a large number of unsaturated

fatty acids such as DPA (docosapentaenoic acid), DHA and EPA (especially EPA and DHA)

which are essential to the human body. It has obvious advantages in preventing

cardiovascular diseases, lowering blood lipids, lowering cholesterol, weight loss, inhibiting

tumour growth and anti-inflammatory effects. Seaweed has a lighter smell, less pollutant

resides, and a higher DHA in comparison to already existing DHA and EPA fish oil oral

health care products. This has made it favourable for consumers and holds an obvious market

advantage. The market demand is rather large with the annual sales volume increasing year by

year. For producers seaweed can use light energy, water and C02 to synthesis a large amount

of organic matter.

SUMMARY OF THE INVENTION

For prior deficiencies, the present invention provides a gel candy to boost children's immune

systems which promotes balanced nutrition and effectively improves their immunity and the

method of preparation.

The purpose of the present invention was achieved through the following technical solutions:

A gel candy to boost children's immunity. Composed of both a shell and its contents.

The content (filling) includes the following components in part: 230 to 250 parts of DHA

algal oil, 8 to 12 parts of N-acetylneuraminic acid, 120 to 140 parts of walnut oil, 10 to 20

parts of lemon oil, 5 to 10 parts of mono-and diglycerides of fatty acids, and 0.2 to 0.4 parts

of steviol glycosides.

Preferably, the content should include the following components by part: 230 parts of DHA

algal oil, 10 parts of N-acetylneuraminic acid, 120 parts of walnut oil, 20 parts of lemon oil, 5

parts of mono-and diglycerides of fatty acids, and steviol glycosides 0.2 parts.

Preferably the content should include the following components by part: 240 parts of DHA

algal oil, 8 parts of N-acetylneuraminic acid, 135 parts of walnut oil, 15 parts of lemon oil, 8

parts of mono-and diglycerides of fatty acids, and steviol glycosides 0.3 copies.

Preferably the content should include the following components by part: 235 parts of DHA

algal oil, 9 parts of N-acetylneuraminic acid, 125 parts of walnut oil, 10 parts of lemon oil, 6

The outer-shell includes the following components by part: 130 to 150 parts of gelatin, 30 to

parts of purified water, 20 to 30 parts of dextrose monohydrate, 20 to 30 parts of maltitol,

to 8 parts of trehalose, white 0.3~0.6 parts of granulated sugar, 50~60 parts of glycerin.

The method of preparation for the immunity-boosting gel candy includes the following steps:

1. Weigh the raw materials according to the above mentioned weight ratio use. Mix the

DHA algal oil, N-acetylneuraminic acid, walnut oil, lemon oil, mono-and diglycerides

of fatty acids and steviol to obtain the crude material. The stirring speed is 1000-3000

rpm and the stirring time is 10-30 minutes.

2. Temporarily store the coarse material contents at a temperature of 30-50°C and filter

it through a 200-mesh screen to obtain the fine contents.

3. Add water to the gelatin tank, then add gelatin, purified water, dextrose

monohydrate, maltitol, trehalose, white granulated sugar. Stir and heat at the same

time, after the dissolution and mixing is completed, add glycerin then stir and mix

completely to obtain the rubber - keep it warm for use. The heating temperature is

40-600 C

4. The fine material obtained in step (2) and the shell obtained in step (3) are then

pressed in a pelletising machine. After the pressing is completed they are shaped, dried,

and polished to obtain the immunity-boosting gel candy. The conditions for controlling

the drying cage during the pressing process are: temperature (15-25°C), relative

humidity (25-35%), and drying time (10-15 hours).

DHA is a polyunsaturated fatty acid that is very important to the human body and is an

important member of the Omega-3 unsaturated fatty acid family. DHA plays a major role in

the growth and maintenance of the nervous system cells. The content of DHA is as high as 20%

in the human cerebral cortex, it accounts for the largest proportion in the retina of the eye at

roughly 50%. With this in mind, it proves essential for children's intellectual and vision

development. DHA algal oil is extracted from marine micro-algae which is relatively safer

when being passed on in the food chain as its EPA content is very low.

The preferred preparation method of the DHA algal oil of the present invention is enzymatic

hydrolysis which is as follows:

The cellulase, amylase, hemicellulase, esterase, mannanase, pectinase, and protease are

prepared according to the ratio, and the mass ratio is :2:1:1:1:1:2, compound Enzyme spare.

Use the conventional method to break the cell wall of the seaweed and add a complex

enzyme of 1.5-2.0% of the seaweed mass with the aid of an ultrasound at pH 7.0 and 42°C for

4-10 hours and hydrolyse it at 45°C for 2-4 hours. The best DHA Algal extraction rate is

17.9-19.1%. Ultrasound assistance is an innovation of the present invention, extraction

without ultrasonic assistance will lower the extraction rate by 2 -3 %.

The seaweed previously mentioned is selected from one of dinoflagellates, green algae,

golden algae, charm or diatoms.

The above-mentioned ultrasonic wave is 25kHz, 200-350W.

After extraction, it is preferable to increase the extraction process, use ethanol as the solvent

and implement one or more pressure mutation methods during the extraction process. The

extraction temperature is 30°C-50°C After the extraction pressure reaches1OMPa-25MPa,

the pressure mutation is implemented to make the algae cells. The crushing is further

completed and the extraction pressure is reduced to 2MPa-5MPa - the extraction time is 1- 8

hours.

N-acetylneuraminic acid is mainly composed of glycoproteins, glycolipids or bacterial

capsular substances in animal cell membranes or secretions. In glycoproteins or glycolipids,

the ketone group at position two, forms a glycosidic bond and it is located at the non-reducing

end of the sugar side chain. Its negative charge or unique chemical structure gives it various

physiological specificities. With anti-bacterial and virus functions, N-acetylneuraminic acid

can be combined with bacteria and viruses, and through a certain mechanism, it can inhibit

and resist. As an important receptor for many pathogenic microorganisms infecting the body,

sialic acid provides important research ideas for the prevention of human infectious diseases.

N-acetylneuraminic aid also has anti-inflammatory and immune enhancements, it can regulate

the anti-inflammatory activity of IgG achieving the effect of immunity-boosting. Baby's first

line of defence that affects their immunity (the barrier effect of the skin and mucous

membrane) can improve the resistance of the mucous membrane of the respiratory track.

In summary, the present invention has the following beneficial effects:

1. The immunity-boosting gel candy effectively promotes children's nutritional

balance and effectively improves their immunity. Moreover, it is made with safe

vegetable oils and has no toxic side effects, is extremely safe, and does not set off

allergies.

2. There is reasonable scientific information which suggests the combination of

N-acetylneuraminic acid and other components are beneficial for the human body to

absorb or use.

3. The gel candy provided by the present invention has a simple preparation method

and is easy to operate.

4. The source of DHA in algal oil is much more suitable for children as it does not

contain EPA.

DESCRIPTION OF PREFFERRED EMBODYMENT

The embodiments of the present invention are intended to be illustrative and not to limit the

scope of the present invention. Any slight difference in the nutrient composition due to the

different milk source should not be used as it may cause an inconsistency between the

embodiment of the present invention and the specification. The present invention aims to

protect the purpose of adding nutrients, rather than to make the ratio particularly strict. The

ground is limited to a specific value.

EMBODIMENT 1

An immunity-boosting gel candy. The gel candy for boosting children's immunity is

composed of a nutrient content filling and an outer-shell.

The contents include the following components by part: 230 parts of DHA algal oil,

N-acetylneuraminum 10 parts of acid, 120 parts of walnut oil, 20 parts of lemon oil, 5 parts of

mono-and diglycerides of fatty acids, and 0.2 parts of steviol glycosides.

The outer-shell includes the following components by part: 150 parts of gelatin, 35 parts of

purified water, 25 parts of dextrose monohydrate, 30 parts of maltitol, 8 parts of trehalose, 0.3

parts of white sugar, and 60 parts of glycerin.

The method of preparation for the children's immunity-boosting gel candy includes the

following steps:

of fatty acids and steviol to obtain the crude material. The stirring speed is 3000 rpm

and the stirring time is 10 minutes.

2. Temporarily store the coarse material contents at a temperature of 50°C and filter it

through a 200-mesh screen to obtain the fine contents.

3. Add water to the gelatin tank, then add gelatin, purified water, dextrose

600 C

the drying cage during the pressing process are: temperature (25C), relative humidity

(25%), and drying time (15 hours).

EMBODIMENT 2

composed of a nutrient content filling and an outer-shell.

The contents include the following components by part: 240 parts of DHA algal oil, 8 parts

of N-acetylneuraminic acid, 135 parts of walnut oil, 15 parts of lemon oil, 8 parts of

mono-and diglycerides of fatty acids, 0.3 parts of steviol glycosides.

The outer-shell includes the following components by part: 140 parts of gelatin, 50 parts of

purified water, 20 parts of dextrose monohydrate, 22 parts of maltitol, 5 parts of trehalose, 0.5

parts of white sugar, and 55 parts of glycerin.

following steps:

of fatty acids and steviol to obtain the crude material. The stirring speed is 1000 rpm

and the stirring time is 30 minutes.

through a 200-mesh screen to obtain the fine contents.

3. Add water to the gelatin tank, then add gelatin, purified water, dextrose

600 C

the drying cage during the pressing process are: temperature (15°C), relative humidity

(25%), and drying time (10 hours).

EMBODIMENT 3

composed of a nutrient content filling and an outer-shell.

The contents include the following components by part: 235 parts of DHA algal oil,

N-acetylneuraminum 9 parts of acid, 125 parts of walnut oil, 10 parts of lemon oil, 6 parts of

mono-and diglycerides of fatty acids, and 0.2 parts of steviol glycosides.

The outer-shell includes the following components by part: 150 parts of gelatin, 50 parts of

purified water, 30 parts of dextrose monohydrate, 22 parts of maltitol, 5 parts of trehalose, 0.3

parts of white sugar, and 50 parts of glycerin.

following steps:

and the stirring time is 20 minutes.

2. Temporarily store the coarse material contents at a temperature of 40°C and filter it

through a 200-mesh screen to obtain the fine contents.

3. Add water to the gelatin tank, then add gelatin, purified water, dextrose

500 C

(25%), and drying time (10 hours).

EMBODIMENT 4

The enzymatic method for extracting DHA algal oil is as follows:

prepared according to the ratio, and the mass ratio is 2:2:1:1:1:1:2, compound Enzyme spare.

Use conventional method to break the cell wall of the seaweed and add a complex enzyme

of 1.5-2.0% of the seaweed mass with the aid of an ultrasound at pH 7.0 and 42C for 4 hours

and hydrolyse it at 450 C for 4 hours. The best DHA Algal extraction rate is 17.9-19.1%.

Ultrasound assistance is an innovation of the present invention, extraction without ultrasonic

assistance will lower the extraction rate by 2-3%.

golden algae, charm or diatoms.

The above-mentioned ultrasonic wave is 25kHz, 350W.

extraction temperature is 50°C After the extraction pressure reaches 25MPa, the pressure

mutation is implemented to make the algae cells. The crushing is further completed and the

extraction pressure is reduced to 5MPa - the extraction time is 8 hours.

EMBODIMENT 5

The enzymatic method for extracting DHA algal oil is as follows:

Use conventional method to break the cell wall of the seaweed and add a complex enzyme of

1.5% of the seaweed mass with the aid of an ultrasound at pH 7.0 and 42°C for 4 hours and

hydrolyse it at 45°C for 2 hours. The best DHA Algal extraction rate is 17.9-19.1%.

assistance will lower the extraction rate by 2 - 3 %.

golden algae, charm or diatoms.

The above-mentioned ultrasonic wave is 25kHz, 200W.

extraction temperature is 30°C After the extraction pressure reaches 1OPa, the pressure mutation is implemented to make the algae cells. The crushing is further completed and the extraction pressure is reduced to 2MPa - the extraction time is 1 hour.

Product Character Detection:

Table 1: Nutritional Component Information

requirement Per 100 g NRV%

energy < 3248kJ 2707 kJ 32%

protein 214.0g 17.5 g 29%

fat 569.6g 58.0 g 97%

DHA >11.9 g 14.9 g

Carbohydrates 212.4g 15.5 g 5%

sodium <247mg 206 mg 10%

The candies obtained in the present invention and commercially available gelatinous candies

are used as comparative examples for quality inspection and comparison. The results are

shown in Table 2 and Table 3. It is made obvious that the gelatinous candies in example 1 and

3 are significantly better in the texture and water index.

Table 2: Texture Analysis (Hardness Unit: G)

Centrifuge time Embodiment 1 Embodiment 2 Embodiment 3 Comparison

Oh Sample hardness 8762.1 8653.5 8692.2 5921.3

0.5h Sample hardness 8025.6 8112.5 8048.7 4031.8 at 900 C

lh Sample hardness at 7563.4 7396.7 7431.6 3735.4 900 C

hardness changes from 8.33% 8.38% 8.02% 21.57% 0 to 0.5h

hardness changes from 13.68% 16.67% 15.21% 31.57% 0.5 to lh

Table 3: Water Loss Analysis (Unit: %)

Centrifuge time Embodiment 1 Embodiment 2 Embodiment 3 Comparison

Oh 0.57 0.54 0.57 0.63

0.5h 0.59 0.58 0.62 0.64

lh 0.63 0.65 0.68 0.75

average 0.60 0.59 0.62 0.67

An experimental study on mice with immune enhancement function of the gel candy at hand:

Test materials: Example 1, Example 2, Example 3. The prepared gel candy was the test

substance.

Blank control: distilled water.

Experimental animals: mice, half male and half female, weighing 18-22 grams, with 10 mice

in each group. The source of feed and litter is the same as above.

The mice were administered ten times the recommenced amount for humans. The

compositions of the examples of this invention were fed for 30 days.

The mice were randomly divided into blank control groups and drug testing groups. Each

group was given intragastric administration once a day according to the dosage and usage.

After 30 days the animals were tested for various immune indexes.

As mentioned the mice divided into groups were given intragastric administration once a day

with a dose of 0.3 grams/kilograms each time. The blank control group were given an equal

volume of distilled water. After 30 consecutive days the animals were tested for various

immune indexes. On the twenty-sixth day of gavage, the mice were sensitised with an

intraperitoneal injection of 2% (v/v) sheep red blood cells - 0.2 millilitre/ mouse. After four

days, the thickness of the left hind foot plantar was measured, and then 20% (v/v) SRBC

(20ul/mouse) was injected subcutaneously at the measurement site. The thickness of the left

hind foot was measured before and 24 hours after the attack. The thickness of the DTH is

expressed by the thickness difference of the foot and plantar before and after the attack, and

the analysis of the variance is performed. The test results are shown in Table 4.

The determination of delayed type allergy (DTH) in mice induced by dinitrofluorobenzene is

shown in table 4.

Table 4: Determination Results of Delayed Type Allergy (DTH) in Mice induced by

Dinitrofluorobenzene.

Embodiment 1 Embodiment 2 Embodiment 3 Comparison

Low Medium High Low Medium High Low Medium High

dose dose dose dose dose dose dose dose dose

Plantar

swelling

degree 24h 0.34 0.38 0.42 0.37 0.40 0.43 0.31 0.36 0.43 0.25 after

injection

(mm)

P value 0.304 0.042 0.013 0.312 0.052 0.017 0.317 0.058 0.024

The results show that under the experimental conditions, the plantar swelling degree of mice

in the middle and high dose groups was significantly higher than in the control group. On the

surface, the medium and high dose products can improve the ability of delayed type allergy in

mice which proves the gel candy of the present invention enhances immunity.

The above are only preferred embodiments of the present invention and are not

interned to limit the present invention in any way. Anyone familiar with the profession

may use the technical content disclosed above to change or modify the equivalent of

equivalent changes. However, any simple modifications, equivalent changes, and

modifications made to the above embodiments based on the technical essence of the

present invention without departing from the content of the technical solution of the

present invention still belong to the protection scope of the technical solution of the

present invention.

Claims

CLAIM

1. An immunity-boosting gel candy for children, characterized by its shell and nutrient content filing; the filling contains the following components in part: 230~250 parts of DHA algal oil, 8-12 parts of N-acetylneuraminic acid, 120-140 parts of walnut oil, 10-20 parts of lemon oil, 5-10 parts of mono-and diglycerides of fatty acids, 0.2-0.4 parts of steviol glycosides.

2. As mentioned in the first claim an immunity boosting gel candy filling which is comprised of the following components in part: 230 parts of DHA algal oil, 10 parts of N-acetylneuraminic acid, 120 parts of walnut oil, 20 parts of lemon oil, 5 parts of mono-and diglycerides of fatty acids, 0.2 parts of steviol glycosides.

3. The method of preparation for a children's immunity-boosting gel candy according to to claims 1 or 2 which is characterised by the following steps:

a) Weigh the raw materials according to the above mentioned weight ratio use; Mix the DHA algal oil, N-acetylneuraminic acid, walnut oil, lemon oil, mono-and diglycerides of fatty acids and steviol to obtain the crude material; The stirring speed is 1000-3000 rpm and the stirring time is 10-30 minutes; b) Temporarily store the coarse material contents at a temperature of 30-50°C and filter it through a 200-mesh screen to obtain the fine contents; c) Add water to the gelatin tank, then add gelatin, purified water, dextrose monohydrate, maltitol, trehalose, white granulated sugar; Stir and heat at the same time, after the dissolution and mixing is completed, add glycerin then stir and mix completely to obtain the rubber - keep it warm for use; The heating temperature is 40-60°C; d) The fine material obtained in step (2) and the shell obtained in step (3) are then pressed in a pelletising machine; After the pressing is completed they are shaped, dried, and polished to obtain the immunity-boosting gel candy; The conditions for controlling the drying cage during the pressing process are: temperature (15-25°C), relative humidity (25-35%), and drying time (10-15 hours).

4. For the method of preparation for a children's immunity-boosting gel candy as mentioned in claim 3, in the first step the stirring speed is 1000-3000 rpm, and the stirring time is 10-30 minutes.

5. For the method of preparation for a children's immunity-boosting gel candy as mentioned in claim 3, in step (3) the heating temperature is 40-60°C