KR20160058389A - Method of Manufacturing Volumizing Antocyanine Probiotics Metabolite and Pharmetics, cosmetics and Juice and food complex - Google Patents

Abstract

The present invention provides: a method for preparing a product by cultivating lactobacilli in an MRS culture medium in which a blueberry, mulberry, black chokeberry (aronia), and a black currant berry, which are functional fruits and vegetables, are mixed by applying a bio-conversion technique; medicines, quasi-drugs, and cosmetics for skin whitening, wrinkle prevention, and anti-aging; a composition used as medicines, quasi-drug, and cosmetics for preventing hair loss; and a method for preparing functional materials and composition materials, which are used as antioxidant and anti-aging beverages, foods and beverages, health supplement foods, and fortifying nutrients.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for producing a lactic acid bacterium cultured product having increased antioxidant activity and a pharmaceutical composition, a quasi-drug, a cosmetic, and a food and beverage composition,

The present invention relates to a method for producing a lactic acid bacterium culture product by culturing a lactic acid bacterium in an MRS medium mixed with functional fruits and vegetables such as blueberry, mulberry [odi], black chocorberry [arnoia] and black currant berries; Whitening, anti-wrinkle, anti-aging medicine, quasi-drug, cosmetic composition; Compositions for use as anti-hair loss medicines, quasi-drugs, and cosmetics; Preparation of functional materials and ingredients for antioxidant and anti-aging beverages, foodstuffs, health supplements and nutritional enhancers

Recently, interest in antioxidation, anti-aging and anti-aging has been growing, and berries such as blueberries are the components of polyphenols, such as the unique anthocyanins of fruits including blueberries, to maintain optimal health and alleviate the symptoms of the disease It is known to have a function to prevent various diseases and chronic diseases. In addition to general food and beverage, tea and juice are consumed as well as health food.

It is because it contains various kinds of potassium, magnesium, iron, calcium, vitamins A, B, C concentration, other minerals, organic acids, and more phenols than other fruits of berries.

Recently, despite the large volume of berries being mass-produced due to the spread of cultivation techniques, it has been found that there is a difficulty in extracting antioxidants from the pulp and seeds, while being sad that there is a limit to the domestic consumption and distribution other than life and consumption.

Lactic acid bacteria are present not only in the organs of animals and humans, but also in soils, grains and many other places, and spores fly in the wind. Normal lactic acid bacteria and microorganisms are almost killed after 1 to 2 hours of incubation. On the other hand, lactic acid bacteria have recently been attracting attention due to the fact that they produce antibiotics through enzymatic process. However, they are not only resistant to wet heat and drying, but also have a weak attainment rate after ingestion into the human body. In addition, it is missioned as a result of experiments under the same strong acid conditions as gastric juice, and is excreted through the metabolism of the animal.

In order to solve the above problems, the present invention has been made to solve the above problems in that the extract of berries is extracted using an enzyme to increase the extraction efficiency, and the extract is mixed with the MRS medium, and the lactic acid bacteria are cultured using the mixture, and Lactobacillus palntarum, Bacillus brevis and Bacillus coitalis B. coagulance were cultured to produce a lactic acid bacterial product containing an antioxidant, anthocyanin, and the solution was prepared by introducing an enzyme process into the cultured lactic acid bacterium culture product and an enzyme process .

       The method for producing a lactic acid bacterium culture product containing an antioxidant according to the present invention comprises:

- Step 1: The berries containing blueberries are washed and sterilized,

- Step 2: Extracts were prepared from 0.01 to 15% pectinase in a berries container containing sterilized blueberries,

Step 3: Lactic acid bacteria are cultured in a mixture of the above extract and the MRS medium to prepare a culture solution

- Step 4: The concentrate is subjected to stationary culture for fermentation [aging] and filtered using a press filter to produce a culture solution

In the fifth step, enzymes and lactic acid bacteria are injected to increase the extraction efficiency of the culture solution, and then the protease is added after fermentation to sterilize the enzyme at a temperature of 80 degrees Celsius or less.

   According to the present invention, an extract of berries including blueberries is prepared, and the enzyme reaction is smoothly performed without adding sugar, and berries concentrate having a high taste and aroma is produced. In addition, the present invention provides a method for producing a lactic acid bacterial product which is simple and cheap in terms of the materials and methods used in the fermentation process. The present invention also provides an anthocyanin, which is an effective ingredient of a lactic acid bacterium culture product, ≪ / RTI >

   On the other hand, phenols and flavonoids of plants are known to enhance physiological functions such as antioxidant, antimicrobial, anticancer activity, anti-aging and anti-diabetic activity through fermentation process. Therefore, in the present invention, in order to maximize the effectiveness of the physiologically active component of the berries, the extract is extracted using an enzyme, and after culturing using a lactic acid bacterium, the concentrate and the dry powder are used for whitening, anti- Anti-hair loss medicines, Quasi-drugs, Cosmetics and anti-diabetic products, Antioxidant functions are evaluated and applied to functional materials.

  What is an antioxidant in the present invention? It is a general term for substances that prevent oxidation (oxidation), and it is known that active oxygen is involved in various diseases and it is getting attention. Among the foods, polyphenols, vitamin C, vitamin E, β-carotene, etc., there is a discussion that the effect of oral intake can be seen.

  It has been found that active oxygen is involved in arteriosclerosis, brain and cardiovascular system disorders, aging and carcinogenesis, and the effects and efficacy of oral antioxidants besides conventional antioxidants are attracting attention.

 Some of the foods contain antioxidant substances. One of them is polyphenol which is a substance having two or more hydroxyl groups (hydroxyl groups). Vitamin C, vitamin E, and β-carotene have antioxidant properties and are called antioxidant vitamins.

 However, the human body is equipped with a complicated structure for removing active oxygen. Therefore, the inventors conducted the invention regarding the relationship with these biological structures and the effectiveness of the antioxidants ingested orally.

Antioxidant power Content per 100 g fresh Vitamin C / Vitamin C 221 mg Vitamin E / Vitamin E 1.4 mg Carotenoid
Carotenoids (beta-carotene equivlents) 160 mg Phenolics 2,160 mg  - Anthocyanins 1,200 mg

There are about 20 species in North America, and there are also jungle trees and corn chestnuts in Korea, all of which can be eaten. In terms of horticulture, blueberries are limited to high bush berry (V. corymbosum) growing up to about 5m in height and low bush berry (V. angustifolium var. Laevifolium) growing up to about 30cm in height . The latter grows especially in the northeastern part of the United States and burns every three years. It grows well in acidic soils and does not grow well in basic and neutral soils.

Breeding is divided into roots, blooms, seeds, and is one of the most important fruit trees. Fruit is almost round, 1 ~ 1.5g in one, dark blue, reddish brown, black, with white powder on the outside. Because it has sweet and sour taste, it eats raw and makes jam, juice, canned food. The fruit of the pure gold tree that grows in Korea also has a large fruit depending on the individual.

The strong antioxidant effect of berries contains strong antioxidant effect, anti-aging immunity, computer, as well as smartphone use, which is effective for eyesight reduction, Inhibition of cardiovascular disease Effect on cardiovascular related diseases Removal of inflammation of blood vessels and increase elasticity to help healthy blood vessels to be formed. It is effectively known for arteriosclerosis. It prevents insulin production, diabetes, hypertension and various adult diseases, improves male sexual function, Of the total number of cases of erectile dysfunction.

It is said that it is normal for men to have an erectile intermittently when they are sleeping if they are both normal and normal. However, especially if an adult male does not have a fever at the dawn or is insignificant, .]

Radiation Hazard Relief Helps to damage and treat the cells due to exposure to radioactive substances and radiation. It has excellent ability to remove active oxygen, improves obesity, helps prevent fat accumulation, prevents fat accumulation, and is effective against obesity. Prevention of colds and influenza Prevention of colds and pro-anthocyanidins to prevent infection again after colds Prevent skin beauty Aging skin free radicals and brain function Strengthen brain function Prevent brain cell damage Parkinson's disease Dementia prevention and memory Helps strengthening, improving digestive function Gastric protection Other efficacy Anti-inflammatory and bactericidal action Powerful antioxidants Proanthocyanidins are known to prevent the reproduction of harmful bacteria such as bacteria that cause cystitis.

Blueberries contain large amounts of prebiotics in the form of monosaccharides and disaccharides. In addition, prebiotics are substances that help to improve the intestinal environment by becoming a nutrient source of probiotics as an indigestible ingredient that helps the growth of beneficial bacteria in the intestines. Prebiotics are often composed of carbohydrates, such as oligosaccharides, and most are present in the form of dietary fiber. Provides a good environment for growth and cultivation of probiotics.

Probiotics refers to living microorganisms that are beneficial to the human body when consumed in moderate quantities. It is a bacterium that is beneficial to our body. Most probiotics known to date are lactic acid bacteria.

In the present invention, Lactobacillus plantarum L.bacillus palntarum, Bacillus brevis, and B. coagulance were cultured and proliferated

      The method for producing a lactic acid bacterium culture product containing an antioxidant according to the present invention comprises the steps of:

- Step 1: Berries containing blueberries are sterilized by washing with purified water sterilized with ozone water,

- Step 2: In a berries container containing sterilized blueberries, 0.01 ~ 15% of pectinase is subjected to enzyme reaction within 40 ~

Third step: 0.1% to 30% [w / w] of lactic acid L. bacillus palntarum, Bacillus brevis, and B. coagulance were injected into a 30% mixture of the above extract and MRS broth medium, After 1 to 4 hours of lactic acid fermentation, the fermentation broth was prepared

- Step 4: The concentrate is subjected to stationary culture for fermentation [aging], and filtered using a press filter to prepare a fermented concentrate

- Fifth step, for extraction efficiency and fermentation of the fermentation concentrate, the enzyme and the complex lactic acid bacteria are secondly injected, sterilized after fermentation, and enzymatically treated with protease, bromelain.

1. Quantitative analysis was carried out. Materials and methods were as follows.

The HPLC grade was used and the samples were extracted at room temperature for one day with 80% MeOH (1 L) supplemented with 0.1% HCl in 100 g of lactobacillus products [blueberry], blueberry (concentrate) and cranberry . The extract was concentrated and weighed to determine the yield. Each extract was diluted to a concentration of 5000 ppm using DW.

 2. Analysis conditions

 Column: Agilent Eclipse XDB-C18 was used for the HPLC condition and 8% formic acid was dissolved in Acetonitrile in the mobile phase.

 Gradient is 05 min, 6% B; / 535 min, 610% B; / 3555 min, 1020% B / 5562 min, 20-25% B / 6265 min, 25100% B / 6570 min, 100% B; / 7072 min, 1006% B; / 7275 min, 6% B / Injection volume: 5 L / Flow rate: 0.4 mL / min / Column temperature: 30C Detector: UV (520 nm)

3. Analysis Results

Cranberry (concentrate) Blueberry (concentrate) Herbal products [blueberries] Sample weight 100.1 g 101.0 g 102.2 g 80% MeOH ext. 5.3 g 9.5 g 10.4 g

The extraction yield was similar to that of lactic acid products [blueberry medium] and blueberry, but the cranberry content was 50% lower. The same chromatogram was obtained with the same concentration (5000 ppm) , The relative anthocyanin content was found to be about 2.6 times higher than that of blueberry concentrate and the lowest in cranberry.

Claims (3)

In the present invention, sterilized blueberry, mulberry [odi], black chocoberry [arnoia] and black currant berries are put into a container to obtain 0.01 ~ 15% of pectinase and 40 Performing an enzymatic reaction within 60 deg. Mixing the extract with a mixture of 10% to 90% of MRS broth medium, mixing the extract with a mixture of 10% to 90%, mixing the mixture with a mixture of 35 to 65 ° C for 1 to 4 hours Fermenting with a lactic acid bacterium to prepare a culture solution; The method according to claim 1, wherein the lactic acid bacterium is at least one selected from the group consisting of plant-derived Lactobacillus acidophilus, Bacillus brevis, and Bacillus coagulans; Culturing the concentrate for 30 minutes at a temperature of 60 ° C for 60 minutes to ferment the concentrate for 24 hours, agitating it, filtering it with a press filter [decanter] to prepare a culture solution, extracting efficiency for cultivation, The second lactic acid bacteria and the enzyme are injected and cultured; And 0.1 to 30% of the protease, bromelain. A pharmaceutical or quasi-cosmetic composition for whitening, anti-wrinkling, or anti-aging use containing 0.1 to 95% by weight of the cultured product of lactic acid bacteria obtained in the present invention in the total weight of the composition; Medicines and quasi-cosmetic cosmetic compositions used for hair loss prevention and hair growth; Atopic and allergic medicines, and quasi-cosmetic cosmetic compositions, which are lactic acid products according to the above-mentioned techniques.