KR20160058389A - Method of Manufacturing Volumizing Antocyanine Probiotics Metabolite and Pharmetics, cosmetics and Juice and food complex - Google Patents

Method of Manufacturing Volumizing Antocyanine Probiotics Metabolite and Pharmetics, cosmetics and Juice and food complex Download PDF

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KR20160058389A
KR20160058389A KR1020140159628A KR20140159628A KR20160058389A KR 20160058389 A KR20160058389 A KR 20160058389A KR 1020140159628 A KR1020140159628 A KR 1020140159628A KR 20140159628 A KR20140159628 A KR 20140159628A KR 20160058389 A KR20160058389 A KR 20160058389A
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lactic acid
quasi
mixture
cosmetic
cosmetics
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Korean (ko)
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정기관
양혜원
정민경
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정기관
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes
    • A23B7/155Microorganisms; Enzymes; Antibiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/45Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/96Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225Lactobacillus
    • C12R2001/25Lactobacillus plantarum

Abstract

The present invention provides: a method for preparing a product by cultivating lactobacilli in an MRS culture medium in which a blueberry, mulberry, black chokeberry (aronia), and a black currant berry, which are functional fruits and vegetables, are mixed by applying a bio-conversion technique; medicines, quasi-drugs, and cosmetics for skin whitening, wrinkle prevention, and anti-aging; a composition used as medicines, quasi-drug, and cosmetics for preventing hair loss; and a method for preparing functional materials and composition materials, which are used as antioxidant and anti-aging beverages, foods and beverages, health supplement foods, and fortifying nutrients.

Description

항산화 물질이 증가된 유산균 배양 생성물의 제조 공법 및 이를 조성으로하는 의약품 및 의약외품,화장품 및 식음료 조성물{ Method of Manufacturing Volumizing Antocyanine Probiotics Metabolite and Pharmetics, cosmetics and Juice and food complex }FIELD OF THE INVENTION [0001] The present invention relates to a method for producing a lactic acid bacterium cultured product having increased antioxidant activity and a pharmaceutical composition, a quasi-drug, a cosmetic, and a food and beverage composition,
본 발명은 바이오 전환기술을 응용하여 기능성 과채류인 블루베리,멀베리[오디],블랙초코베리[아로니아],블랙 커렌트 베리가 혼합된 MRS배지에서 유산균 배양하여 유산균 배양 생성물을 제조하는 방법; 미백,주름 방지, 항노화용 의약품,의약외품,화장료 조성물; 탈모방지용 의약품,의약외품,화장료로 사용되는 조성물; 항산화 및 항 노화용 음료, 식료품, 건강보조식품용 및 영양강화제로 사용하는 기능성 물질과 조성료의 제조방법The present invention relates to a method for producing a lactic acid bacterium culture product by culturing a lactic acid bacterium in an MRS medium mixed with functional fruits and vegetables such as blueberry, mulberry [odi], black chocorberry [arnoia] and black currant berries; Whitening, anti-wrinkle, anti-aging medicine, quasi-drug, cosmetic composition; Compositions for use as anti-hair loss medicines, quasi-drugs, and cosmetics; Preparation of functional materials and ingredients for antioxidant and anti-aging beverages, foodstuffs, health supplements and nutritional enhancers
최근에 항산화,항노화,안티에이징에 관심이 커지고 있고, 블루베리 등 베리류 과일은 블루 베리를 포함한 과일 류의 독특한 안토시아닌 등 폴리페놀의 구성 요소로 최적의 건강을 유지하고 질병의 증상을 완화하는 데 도움을 주며,이로 인해, 다양한 질병 및 만성 질병을 방지기능이 있는것으로 알려 져있다. 일반 식음료와 뿐만 아니라, 차와 주스 뿐만 아니라 건강식품으로 소비되고있다. Recently, interest in antioxidation, anti-aging and anti-aging has been growing, and berries such as blueberries are the components of polyphenols, such as the unique anthocyanins of fruits including blueberries, to maintain optimal health and alleviate the symptoms of the disease It is known to have a function to prevent various diseases and chronic diseases. In addition to general food and beverage, tea and juice are consumed as well as health food.
베리류 다른 과일보다, 여러 종류의 칼륨, 마그네슘, 철, 칼슘, 비타민 A, B, C의 농도, 다른 미네랄, 유기산, 그리고 더 많은 페놀류을 포함하기 때문이다.가장 매력적인 기능은 독특한 맛과 향입니다.It is because it contains various kinds of potassium, magnesium, iron, calcium, vitamins A, B, C concentration, other minerals, organic acids, and more phenols than other fruits of berries.
최근, 재배기술의 보급으로 다량의 베리류가 양산되고 있음에도 생과 소비 이외는 국내소비 및 보급에 한계가 있음을 아쉬워하고 있던 중, 과육과 씨앗에서 항산화 물질의 추출의 어려움이 있다는 점을 알게 되었다.Recently, despite the large volume of berries being mass-produced due to the spread of cultivation techniques, it has been found that there is a difficulty in extracting antioxidants from the pulp and seeds, while being sad that there is a limit to the domestic consumption and distribution other than life and consumption.
유산균은 동물과 인체의 장기뿐마아니라, 토양, 곡류, 기타 여러 곳에 존재하고, 포자는 바람에 날려 다닌다. 일반 유산균 및 미생물이 배양 1~2시간 후 거의 사멸된다. 한편, 유산균이 효소 공정을 통하여 항생 물질을 생산하는 것이 밝혀져 최근 주목을 받고 있으나, 습열·건조에 대한 저항성이 약할 뿐 아니라 인체에 섭취된 후 장까지의 도달률도 미약한 것으로 밝혀졌다. 또한, 위액과 동일한 강산성 조건에서 실험한 결과 사명되며, 동물체의 대사 활동을 통해 체외로 배출된다.Lactic acid bacteria are present not only in the organs of animals and humans, but also in soils, grains and many other places, and spores fly in the wind. Normal lactic acid bacteria and microorganisms are almost killed after 1 to 2 hours of incubation. On the other hand, lactic acid bacteria have recently been attracting attention due to the fact that they produce antibiotics through enzymatic process. However, they are not only resistant to wet heat and drying, but also have a weak attainment rate after ingestion into the human body. In addition, it is missioned as a result of experiments under the same strong acid conditions as gastric juice, and is excreted through the metabolism of the animal.
본 발명에서는 상기의 문제 점을 해결하기 위하여, 베리류 과일을 효소를 이용하여 추출하여 추출 효율을 높였고,추출물과 MRS 배지를 혼합하고, 이를 이용하여 유산균을 배양하고, 락토바실러스플란타룸 Lactobacillus palntarum, 바실루스 브레비스 Bacillus brevis, 바실러스 코아귤런스B.coagulance를 배양 함으로서, 항산화물질인 안토시아닌을 함유한 유산균 생성물을 제조하고, 유산균 배양액과 배양생성물을 효소 공정을 도입하여 펩타이드화함으로서 그 해결 방법을 제공하는데 목적이 있다. In order to solve the above problems, the present invention has been made to solve the above problems in that the extract of berries is extracted using an enzyme to increase the extraction efficiency, and the extract is mixed with the MRS medium, and the lactic acid bacteria are cultured using the mixture, and Lactobacillus palntarum, Bacillus brevis and Bacillus coitalis B. coagulance were cultured to produce a lactic acid bacterial product containing an antioxidant, anthocyanin, and the solution was prepared by introducing an enzyme process into the cultured lactic acid bacterium culture product and an enzyme process .
본 발명에 의한 항산화 물질이 함유된 유산균 배양 생성물의 제조방법은,        The method for producing a lactic acid bacterium culture product containing an antioxidant according to the present invention comprises:
- 제1공정 : 블루베리를 포함한 베리류를 세척하여 살균하고 , - Step 1: The berries containing blueberries are washed and sterilized,
- 제2공정 : 살균 한 블루베리를 포함한 베리류 용기에 담고 0.01~15%의 pectinase를 이용하여 추출액을 제조하고 , - Step 2: Extracts were prepared from 0.01 to 15% pectinase in a berries container containing sterilized blueberries,
- 제3공정 : 상기 추출액과 MRS배지를 혼합액을 유산균을 배양하여, 배양액을 제조한후Step 3: Lactic acid bacteria are cultured in a mixture of the above extract and the MRS medium to prepare a culture solution
- 제4공정 : 상기 농축액을 바이오전환 위해 정치 배양[숙성]하여 프레스 필터를 이용해 여과하여 배양액을 제조하고- Step 4: The concentrate is subjected to stationary culture for fermentation [aging] and filtered using a press filter to produce a culture solution
- 제5공정, 이 배양액의 추출효율증가 및 배양을 위해 효소와 유산균을 주입하여 발효후 프로테아제를 투입하여 효소반응후 80도씨 이하에서 살균하는 것으로 구성된다.In the fifth step, enzymes and lactic acid bacteria are injected to increase the extraction efficiency of the culture solution, and then the protease is added after fermentation to sterilize the enzyme at a temperature of 80 degrees Celsius or less.
본 발명에 의한, 블루베리를 포함한 베리류 추출물을 제조하되, 설탕을 첨가하지 않고도 효소반응이 원활히 이루어지도록 하였며, 맛과 향이 좋아 소비자들의 전반적으로 선호도가 높은 베리류 농축액이 제조된다. 또한, 발효 과정시 사용된 재료 및 방법이 간단하여 비용 면에서 저렴한 유산균 생성물의 제조방법이 제공된다.또한, 본 발명은 유산균 배양 생성물의 유효성분인 안토시아닌이 증가되어, 항산화제가 함유된 유산균 배양 생성물을 획득하는 것을 제공한다.   According to the present invention, an extract of berries including blueberries is prepared, and the enzyme reaction is smoothly performed without adding sugar, and berries concentrate having a high taste and aroma is produced. In addition, the present invention provides a method for producing a lactic acid bacterial product which is simple and cheap in terms of the materials and methods used in the fermentation process. The present invention also provides an anthocyanin, which is an effective ingredient of a lactic acid bacterium culture product, ≪ / RTI >
한편, 식물이 가진 페놀류 및 flavonoids는 발효과정을 통해 항산화, 항미생물, 항암활성,항노화,항 당뇨 등의 생리적 기능을 향상시킬 수 있는 것으로 알려져 있다. 따라서 본 발명에서는 베리류가 가진 생리활성 성분 유효성을 극대화하기 위하여 효소를 이용하여 추출하였고, 유산균주를 이용하여 배양과정을 진행한 후, 그 농축액, 건조 분말을 이용하여 미백, 주름 방지, 항노화, 탈모방지용 의약품,의약외품,화장료 및 항 당뇨제품,항산화 기능를 평가하여 기능 소재로의 응용 가능성을 제공한다.   On the other hand, phenols and flavonoids of plants are known to enhance physiological functions such as antioxidant, antimicrobial, anticancer activity, anti-aging and anti-diabetic activity through fermentation process. Therefore, in the present invention, in order to maximize the effectiveness of the physiologically active component of the berries, the extract is extracted using an enzyme, and after culturing using a lactic acid bacterium, the concentrate and the dry powder are used for whitening, anti- Anti-hair loss medicines, Quasi-drugs, Cosmetics and anti-diabetic products, Antioxidant functions are evaluated and applied to functional materials.
본 발명에서 항산화 물질[ antioxidant, 抗酸化物質 ]이란? 산화(酸化)를 방지하는 물질의 총칭인데, 이는 각종 질환에 활성산소가 관여한다는 것이 알려져 주목을 받기 시작했다. 식품 중에는 폴리페놀, 비타민 C, 비타민 E, β-카로틴 등이 있는데, 경구적 섭취로 효과를 볼 수 있다는 논의가 있다.  What is an antioxidant in the present invention? It is a general term for substances that prevent oxidation (oxidation), and it is known that active oxygen is involved in various diseases and it is getting attention. Among the foods, polyphenols, vitamin C, vitamin E, β-carotene, etc., there is a discussion that the effect of oral intake can be seen.
동맥경화나 뇌·심장혈관계 장애, 노화나 발암에 활성산소가 관여한다는 사실이 밝혀져, 기존의 산화방지제 외에 경구적으로 섭취하는 항산화물질의 효과·효능 등이 최근 주목을 받고 있다.  It has been found that active oxygen is involved in arteriosclerosis, brain and cardiovascular system disorders, aging and carcinogenesis, and the effects and efficacy of oral antioxidants besides conventional antioxidants are attracting attention.
식품 중에는 항산화 기능을 갖고 있는 여러 가지 물질이 포함되어 있다. 수산기(水酸基)를 2개 이상 갖고 있는 물질인 폴리페놀(polyphenol) 화합물도 그 중 하나이다. 비타민 C, 비타민 E, β-카로틴 등은 항산화 기능을 갖고 있어 항산화성 비타민이라고 한다. Some of the foods contain antioxidant substances. One of them is polyphenol which is a substance having two or more hydroxyl groups (hydroxyl groups). Vitamin C, vitamin E, and β-carotene have antioxidant properties and are called antioxidant vitamins.
그러나, 인체에는 활성산소를 제거하도록 된 복잡한 구조가 갖추어져 있다. 따라서 이런 생체 구조들과의 관계와 더불어 경구적으로 섭취하는 항산화물질의 유효성에 대해서는 발명을 수행하였다. However, the human body is equipped with a complicated structure for removing active oxygen. Therefore, the inventors conducted the invention regarding the relationship with these biological structures and the effectiveness of the antioxidants ingested orally.
항산화력Antioxidant power Content per 100 g freshContent per 100 g fresh
비타민 c/ Vitamin CVitamin C / Vitamin C 221 mg221 mg
비타민 E / Vitamin EVitamin E / Vitamin E 1.4 mg1.4 mg
카로티노이드
Carotenoids(beta-carotene equivlents)
Carotenoid
Carotenoids (beta-carotene equivlents)
160 mg160 mg
폴리페놀PhenolicsPhenolics 2,160 mg2,160 mg
- 안토시아닌 Anthocyanins - Anthocyanins 1,200 mg1,200 mg
북아메리카 원산으로 20여 종이 알려져 있고 한국에도 정금나무·산앵두나무 등이 있으며 모두 열매를 먹을 수 있다. 원예학적인 면에서 블루베리는 높이가 5m 내외로 자라는 하이부시베리(high bush berry:V. corymbosum)와 높이가 30cm 내외로 자라는 로부시베리(low bush berry:V. angustifolium var. laevifolium)로 한정된다. 후자는 특히 미국 북동부에서 많이 재배하고 매 3년마다 불로 태우면서 가꾼다. 산성토양에서 잘 자라며, 염기성과 중성토양에서는 잘 자라지 않는다. There are about 20 species in North America, and there are also jungle trees and corn chestnuts in Korea, all of which can be eaten. In terms of horticulture, blueberries are limited to high bush berry (V. corymbosum) growing up to about 5m in height and low bush berry (V. angustifolium var. Laevifolium) growing up to about 30cm in height . The latter grows especially in the northeastern part of the United States and burns every three years. It grows well in acidic soils and does not grow well in basic and neutral soils.
번식은 뿌리나누기·휘묻이·종자로 하며 중요한 과수의 하나이다. 열매는 거의 둥글고 1개가 1∼1.5g이며 짙은 하늘색, 붉은빛을 띤 갈색, 검은색이고 겉에 흰가루가 묻어 있다. 달고 신맛이 약간 있기 때문에 날것으로 먹기도 하고 잼·주스·통조림 등을 만든다. 한국에서 자라는 정금나무의 열매도 개체에 따라서는 열매가 큰 것이 있다.Breeding is divided into roots, blooms, seeds, and is one of the most important fruit trees. Fruit is almost round, 1 ~ 1.5g in one, dark blue, reddish brown, black, with white powder on the outside. Because it has sweet and sour taste, it eats raw and makes jam, juice, canned food. The fruit of the pure gold tree that grows in Korea also has a large fruit depending on the individual.
베리류에 함유된 안토시아닌은 강력한 항산화효과 간기능 강화, 노화방지 면역력 증가,컴퓨터는 물론 스마트폰사용으로 저하된 눈건강을 위해 로돕신의 재합성을 통해 눈피로 시력저하 백내장 등에 효과적이며, 아로니아는 콜레스테롤 억제 심장질환심혈관 관련 질병에 효능 혈관의 염증을 제거하고 탄력성을 증가시켜 건강한 혈관을 만드는 것을 도와 동맥경화에도 효과적으로 알려져 있으며,인슐린 생성량, 당뇨병, 고혈압 등 각종 성인병 예방 및 남성 성기능 향상, 혈류부족으로 발생하는 발기부전 개선에 도움을 준다.구체적인 사례는 정력증강, 발기 문제 개선이 가장 많았다. The strong antioxidant effect of berries contains strong antioxidant effect, anti-aging immunity, computer, as well as smartphone use, which is effective for eyesight reduction, Inhibition of cardiovascular disease Effect on cardiovascular related diseases Removal of inflammation of blood vessels and increase elasticity to help healthy blood vessels to be formed. It is effectively known for arteriosclerosis. It prevents insulin production, diabetes, hypertension and various adult diseases, improves male sexual function, Of the total number of cases of erectile dysfunction.
남성의 경우 어린 아이나 어른 모두 정상적이라면 잠을 자면서 간헐적으로 발기가 되는 것이 정상이라고 알려져 있습니다.그런데,특히 성인남성이 새벽에 발기되는 현상이 없거나 미미하다면 병원진단을 받아보는 것이 심각한 문제를 막을 수 있습니다.] It is said that it is normal for men to have an erectile intermittently when they are sleeping if they are both normal and normal. However, especially if an adult male does not have a fever at the dawn or is insignificant, .]
방사능 유해성 완화 방사능물질과 방사선 피폭으로 인한 세포손상 및 치료에 도움을 주며, 활성산소 제거능력이 뛰어나며, 비만체질개선 지방분해작용과 지방이 쌓이지 않게 도와주어 비만에 효과적이며, 감기독감예방 박테리아바이러스 등으로 인한 감기와 독감예방은 떫은 맛을 내는 프로안토시아니딘이 감기 후 재차 감염을 막아주며, 예방 피부미용 피부노화물질인 프리라디칼과 뇌기능 강화 뇌세포손상 방지물 파킨슨씨병 치매뇌졸중 예방 및 기억력 강화에 도움을 주며, 소화기능개선 위보호기타 효능 소염 및 살균작용강력한 항산화프로안토시아니딘은 방광염을 일으키는 균과 같은 유해균을 번식을막아준다고 알려져 있습니다. Radiation Hazard Relief Helps to damage and treat the cells due to exposure to radioactive substances and radiation. It has excellent ability to remove active oxygen, improves obesity, helps prevent fat accumulation, prevents fat accumulation, and is effective against obesity. Prevention of colds and influenza Prevention of colds and pro-anthocyanidins to prevent infection again after colds Prevent skin beauty Aging skin free radicals and brain function Strengthen brain function Prevent brain cell damage Parkinson's disease Dementia prevention and memory Helps strengthening, improving digestive function Gastric protection Other efficacy Anti-inflammatory and bactericidal action Powerful antioxidants Proanthocyanidins are known to prevent the reproduction of harmful bacteria such as bacteria that cause cystitis.
또한, 블루 베리류에는 단당,이당류 형태의 프리바이오틱스를 다량함유하고 있습니다. 또한, 프리바이오틱스(prebiotics)는 장내 유익한 박테리아의 생장을 돕는 난소화성 성분으로써 프로바이오틱스의 영양원이 되어 장내 환경을 개선하는 데 도움을 주는 물질을 말한다. 프리바이오틱스는 올리고당과 같이 탄수화물로 이루어져 있는 경우가 많고, 대부분이 식이섬유의 형태로 존재한다. 프로바이오틱스 성장과 배양에 좋은 환경을 제공하고 있습니다.Blueberries contain large amounts of prebiotics in the form of monosaccharides and disaccharides. In addition, prebiotics are substances that help to improve the intestinal environment by becoming a nutrient source of probiotics as an indigestible ingredient that helps the growth of beneficial bacteria in the intestines. Prebiotics are often composed of carbohydrates, such as oligosaccharides, and most are present in the form of dietary fiber. Provides a good environment for growth and cultivation of probiotics.
프로바이오틱스(Probiotics)는 적당량을 섭취했을 때 인체에 이로움을 주는 살아있는 미생물을 총칭하는 말로 우리 몸에 유익(有益)을 주는 균(菌)을 말한다. 현재까지 알려진 대부분의 프로바이오틱스는 유산균이다. Probiotics refers to living microorganisms that are beneficial to the human body when consumed in moderate quantities. It is a bacterium that is beneficial to our body. Most probiotics known to date are lactic acid bacteria.
본 발명에서는 락토바실러스 플란타룸L.bacillus palntarum, 바실루스 브레비스 Bacillus brevis,코아귤런스B.coagulance를 배양.증식하였다In the present invention, Lactobacillus plantarum L.bacillus palntarum, Bacillus brevis, and B. coagulance were cultured and proliferated
본 발명에 의한 항산화물질이 함유된 유산균 배양 생성물의 세부 제조방법은,       The method for producing a lactic acid bacterium culture product containing an antioxidant according to the present invention comprises the steps of:
- 제1공정 : 블루베리를 포함한 베리류를 오존수로 살균한 정제수로 세척하여 살균하고 , - Step 1: Berries containing blueberries are sterilized by washing with purified water sterilized with ozone water,
- 제2공정 : 살균 한블루베리를 포함한 베리류 용기에 담고 0.01~15%의 pectinase를 40~도 이내에서 효소반응을 하여 5~10Brix의 추출물을 제조하고 , - Step 2: In a berries container containing sterilized blueberries, 0.01 ~ 15% of pectinase is subjected to enzyme reaction within 40 ~
- 제3공정 : 상기 추출물과 MRS broth 배지 30% 혼합액에 유산주L.bacillus palntarum,바실루스 브레비스 Bacillus brevis, B.coagulance 0.1%내지 30%[w/w]를 주입하여, 35~65도씨 에서 1 ~ 4 시간동안 유산균 발효하여, 발효액을 제조한후Third step: 0.1% to 30% [w / w] of lactic acid L. bacillus palntarum, Bacillus brevis, and B. coagulance were injected into a 30% mixture of the above extract and MRS broth medium, After 1 to 4 hours of lactic acid fermentation, the fermentation broth was prepared
- 제4공정 : 상기 농축액을 바이오전환 위해 정치배양[숙성]하여, 프레스 필터를 이용해 여과하여 발효농축액을 제조하고- Step 4: The concentrate is subjected to stationary culture for fermentation [aging], and filtered using a press filter to prepare a fermented concentrate
- 제5공정, 이 발효농축액의 추출효율 및 발효를 위해 효소와 복합유산균을 2차주입하여 발효후 살균한후, 프로테아제인 브로멜라인으로 효소처리하는 것으로 구성된다.- Fifth step, for extraction efficiency and fermentation of the fermentation concentrate, the enzyme and the complex lactic acid bacteria are secondly injected, sterilized after fermentation, and enzymatically treated with protease, bromelain.
Figure pat00001
Figure pat00001
1. 정량분석을 실시하였다.재료 및 방법은 아래와 같다.1. Quantitative analysis was carried out. Materials and methods were as follows.
용매는 HPLC grade를 사용하였으며, 유산균생산물[블루베리], 블루베리 (농축액), 그리고 크랜베리 100 g을 0.1% HCl이 첨가된 80% MeOH(1 L)로 상온에서 하루 동안 추출 하여 시료를 전처리 하였다. 추출물을 농축하여 무게를 측정하여 수율을 확 인하였다. 각 추출물을 D.W.를 이용하여 5000ppm농도로 희석하여 분석 하였음 The HPLC grade was used and the samples were extracted at room temperature for one day with 80% MeOH (1 L) supplemented with 0.1% HCl in 100 g of lactobacillus products [blueberry], blueberry (concentrate) and cranberry . The extract was concentrated and weighed to determine the yield. Each extract was diluted to a concentration of 5000 ppm using DW.
2. 분석조건 2. Analysis conditions
HPLC condition은 Column: Agilent Eclipse XDB-C18를 사용하였으며, Mobile phase는 8% Formic acid를 Acetonitrile에 용해시켜 사용하였다. Column: Agilent Eclipse XDB-C18 was used for the HPLC condition and 8% formic acid was dissolved in Acetonitrile in the mobile phase.
Gradient는 05 min, 6% B;/ 535 min, 610% B; / 3555 min, 1020% B;/ 5562 min, 20-25% B;/ 6265 min, 25100% B;/ 6570 min, 100% B; / 7072 min, 1006% B; / 7275 min, 6% B / Injection volume: 5 L / Flow rate: 0.4 mL/min / Column temperature: 30C이며, Detector : UV (520 nm)로 하였다 Gradient is 05 min, 6% B; / 535 min, 610% B; / 3555 min, 1020% B / 5562 min, 20-25% B / 6265 min, 25100% B / 6570 min, 100% B; / 7072 min, 1006% B; / 7275 min, 6% B / Injection volume: 5 L / Flow rate: 0.4 mL / min / Column temperature: 30C Detector: UV (520 nm)
3.분석결과3. Analysis Results
크랜베리(농축액)Cranberry (concentrate) 블루베리(농축액)Blueberry (concentrate) 유산생성물[블루베리]Herbal products [blueberries]
시료무게Sample weight 100.1 g100.1 g 101.0 g101.0 g 102.2 g102.2 g
80% MeOH ext.80% MeOH ext. 5.3 g5.3 g 9.5 g9.5 g 10.4 g10.4 g
Figure pat00002
Figure pat00002
Figure pat00003
Figure pat00003
Figure pat00004
Figure pat00004
추출 수율은 유산생성물[블루베리배지]와 블루베리와 비슷한 수율을 보였으나 크랜베리는 50%가량 낮았습니다.동일 농도 (5000 ppm)로 측정한 결과 위의 그래프인 chromatogram을 얻었으며, 이들의 peak area를 합산하여 상대적인 anthocyanin함량을 알아본 결과 유산생성물[블루베리배지]가 블루베리 농축액 보다 약 2.6배 높았으며 크랜베리가 가장 낮았습니다.The extraction yield was similar to that of lactic acid products [blueberry medium] and blueberry, but the cranberry content was 50% lower. The same chromatogram was obtained with the same concentration (5000 ppm) , The relative anthocyanin content was found to be about 2.6 times higher than that of blueberry concentrate and the lowest in cranberry.

Claims (3)

  1. 본 발명에서 유산균 생성물을 획득하는 단계에서 살균 한 블루베리,멀베리[오디],블랙초코베리[아로니아],블랙 커렌트 베리를 베리류 생과나 농축액을 용기에 담고 0.01~15%의 pectinase를 40도내지 60도 이내에서 효소반응을 하는 단계; 상기혼합물을 여과, 농축하여 5~10Brix의 추출물을 제조하는 단계;상기 추출물에 MRS broth 배지 10%내지 90%의 혼합물로 혼합하는 단계;상기 침적단계후,35~65도씨 에서 1 ~ 4 시간동안 유산균으로 발효하여, 배양액을 제조하는 단계;In the present invention, sterilized blueberry, mulberry [odi], black chocoberry [arnoia] and black currant berries are put into a container to obtain 0.01 ~ 15% of pectinase and 40 Performing an enzymatic reaction within 60 deg. Mixing the extract with a mixture of 10% to 90% of MRS broth medium, mixing the extract with a mixture of 10% to 90%, mixing the mixture with a mixture of 35 to 65 ° C for 1 to 4 hours Fermenting with a lactic acid bacterium to prepare a culture solution;
  2. 제 1항에 있어서, 유산균은 식물유래 락토바실러스 플란타룸(Lactobacillus acidophillus), 바실루스 브레비스(Bacillus brevis),바실러스 코아귤런스(B. Coagulance) 로 이루어지는 군에서 선택된 적어도 하나인 것임을 특징으로 하는 단계 ; 상기 농축액을 바이오전환 위해 30도내지 60도 이내에서 24시간 정치배양[숙성]하여, 프레스 필터[데칸터]를 이용해 여과하여 배양액을 제조하는 단계;이 배양액의 제조를 위한 추출 효율 및 배양약 제조를 위해 효소와 복합유산균을 2차주입하여 배양하는 단계; 프로테아제인 브로멜라인0.1~30%로 효소 처리하는 것으로 구성된다.The method according to claim 1, wherein the lactic acid bacterium is at least one selected from the group consisting of plant-derived Lactobacillus acidophilus, Bacillus brevis, and Bacillus coagulans; Culturing the concentrate for 30 minutes at a temperature of 60 ° C for 60 minutes to ferment the concentrate for 24 hours, agitating it, filtering it with a press filter [decanter] to prepare a culture solution, extracting efficiency for cultivation, The second lactic acid bacteria and the enzyme are injected and cultured; And 0.1 to 30% of the protease, bromelain.
  3. 본 발명에서의 획득된 유산균 배양 생성물을 조성물 총중량에 대해서 0.1~ 95중량% 함유하는 미백,주름 방지,항노화용으로 사용되는 의약품,의약외품 화장료 조성물; 탈모방지 및 육모용으로 사용되는 의약품,의약외품 화장료 조성물; 아토피 및 알러지용 의약품,의약외품 화장료 조성물에 있어서, 상기 기술에 의한 유산생성물인 것임을 특징으로 하는 의약품,의약외품 화장료 조성물을 말한다.A pharmaceutical or quasi-cosmetic composition for whitening, anti-wrinkling, or anti-aging use containing 0.1 to 95% by weight of the cultured product of lactic acid bacteria obtained in the present invention in the total weight of the composition; Medicines and quasi-cosmetic cosmetic compositions used for hair loss prevention and hair growth; Atopic and allergic medicines, and quasi-cosmetic cosmetic compositions, which are lactic acid products according to the above-mentioned techniques.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018084528A1 (en) * 2016-11-03 2018-05-11 주식회사 쎌바이오텍 Antioxidant and skin improvement composition
KR20190063795A (en) * 2017-11-30 2019-06-10 재단법인 순창군건강장수연구소 Method for producing aronia fermentation product using Lactobacillus plantarum MIFI-SY3 strain
KR102106440B1 (en) * 2018-10-30 2020-05-07 안국건강 주식회사 Composition for improving skin condition comprising blueberry and black rice extract fermented lactic acid bacteria
KR20200065513A (en) * 2018-11-30 2020-06-09 김복선 Fermented aronia beverage and manufacturing method the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018084528A1 (en) * 2016-11-03 2018-05-11 주식회사 쎌바이오텍 Antioxidant and skin improvement composition
KR20180049729A (en) * 2016-11-03 2018-05-11 주식회사 쎌바이오텍 Composition for antioxidation and improvement of skin conditions
KR20190063795A (en) * 2017-11-30 2019-06-10 재단법인 순창군건강장수연구소 Method for producing aronia fermentation product using Lactobacillus plantarum MIFI-SY3 strain
KR102106440B1 (en) * 2018-10-30 2020-05-07 안국건강 주식회사 Composition for improving skin condition comprising blueberry and black rice extract fermented lactic acid bacteria
KR20200065513A (en) * 2018-11-30 2020-06-09 김복선 Fermented aronia beverage and manufacturing method the same

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