KR101921296B1 - Method of fermenting herbs using media and the media - Google Patents

Abstract

A medicament for herb medicine fermentation comprising 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin, And a method for producing an extract of fermented herbal medicine using the same. The median of the present invention is produced by mixing 5 kinds of soil, so that it has many pores and richly contains minerals. Therefore, when used in a process of fermenting herbal medicine, it promotes the growth of microorganisms and promotes fermentation.

Description

The present invention relates to a medicament for fermenting herbal medicine,

The present invention relates to a herbal fermentation method, and more particularly, to a method for producing a herbal fermentation broth such as a goat ginseng, a white ginseng, a gugija, and a media for fermentation using the medicament.

Most effective ingredients of ginseng and other herbal medicines are saponin, glycoside, polysaccharide, flavonoid, essential oil, etc. These efficacious substances may have their own effects, but they are not effective through secondary metabolism by intestinal microorganisms Are often displayed or become available in the human body.

Representative ginseng saponin does not produce its own effect, but it is decomposed and absorbed into saponin metabolism in the human body after ingestion, thus exhibiting its drug efficacy. However, it is known that many saponin components contained in ginseng are excreted without being decomposed or absorbed without intestinal microorganisms capable of decomposing them. In the case of red ginseng, it is known that ginseng saponin is converted into an absorbable ingredient in the human body to increase the efficacy of ginseng. However, it is known that red ginseng saponin contained in red ginseng is decomposed only in about 70% of human stomach or intestines and the rest is not decomposed and absorbed without intestinal microorganisms. Considering this fact, fermentation can be considered as one of the ways to increase the efficacy of herbal medicine.

Ginseng has been a favorite herb of mystery for a long time, and it has grown mainly in mountainous areas of Korea, but it is mostly cultivated now. Ginseng is an important medicinal ingredient of Chinese medicine, and it is prescribed and used for many purposes such as a chemical digestive agent, a branching agent, a dressing agent, an analgesic agent and a nourishing tonic agent. The efficacy of ginseng is reported to include basic metabolic enhancement, promotion of protein synthesis in vivo, resistance to anemia, diabetes and hypertension, fatigue recovery, gastrointestinal function enhancement, brain function enhancement, immune function enhancement, aging suppression, .

Despite the above-mentioned effects, the ginseng product manufactured by the conventional processing process has an incense similar to that of the ginseng, that is, a smell similar to that of the earth, and thus tends to be avoided by women, adolescents and especially foreigners. Consumer preference for the medicinal herbs has changed to a pattern emphasizing efficacy and safety as health food, and the preference of consumers is shifting to favoring non - polluted wild ginseng grown in the mountain rather than ginseng grown in the noji.

In accordance with these changes, there has been an attempt to cultivate ginseng ginseng in the native habitat of ginseng. When the content of saponin, the major component of ginseng, was compared, the 4 - year - old ginseng cultivated in mountain area was 1.3 times higher than the 4 - year - old ginseng cultivated in general field, and the ginseng 3 - year - There is also a report.

Dried roots of white liquor come in a large amount and are called "white liquor". The large mockery is a Korean native plant, also known as the 'mockery', which is light yellowish-green blossomed and grows mainly on the foot of mountains, in sunny meadows, or on the slopes of the coast. It is harvested in the winter, dried and used as a medicinal medicine. It is said that it is used in oriental medicine to help the body to become healthier, to help the hematopoietic function, to help the digestive system, and to detoxify. It is known that it is effective in the case of shortness of limbs, lethargy, sleep disturbance, lack of milk during breastfeeding, and also in white-headed white head (烏 鬚 红) compared to its age. Especially, it is known that it has excellent effects on women's menopausal symptoms, and it is now widely used as a form of health food.

Gugija is a dried deciduous shrub, which is a deciduous shrub belonging to the branch, and it is said to enhance the energy in one room, especially to reduce fatigue and reduce blood pressure. I drink tea in the water in the month. Ingredients include betaine, zeazanthin, carotene, thiamine, and vitamin A, B1, B2, and C.

Currently, various products such as goat ginseng tea, extract, powder, jelly, candy, and goat ginseng have been developed. However, most of them are limited to the conventional ginseng products. Therefore, it is necessary to improve the factors that make the ingestion difficult, such as the unique flavor and flavor of the goat ginseng, and development of a processing method capable of increasing the effectiveness of the ginseng ginseng is also required. Furthermore, it is required to develop a processing method which can increase the efficacy while facilitating the ingestion of medicinal herbs which are widely used at present, such as Pseudomonas sp.

In relation to this, a technology for fermenting a ginseng ginseng to maximize an effective ingredient contained in the ginseng of the ginseng and increase the effect upon ingestion is disclosed in Korean Patent Publication Nos. 10-1440469 and 10-0884044. Korean Patent Laid-Open Publication No. 10-2016-0074300 discloses a method of fermenting a herbal medicinal material to prepare it as a raw material for health food. In Korean Patent Registration No. 10-1100278, a method for producing fermented herbal medicinal extract and a herbal medicine Discloses a composition for preventing or treating neurodegenerative diseases containing fermented extract as an active ingredient.

Korean Patent Registration No. 10-0522445 Korean Patent Publication No. 10-1440469 Korean Patent Publication No. 10-2016-0074300 Korean Patent Publication No. 10-1100278

The object of the present invention is to provide a medicament capable of promoting fermentation in the process of fermenting herbal medicines such as goat ginseng, white pheasant, and gugija using microorganisms, and to provide a method for effectively fermenting herbal medicines using this medicament do.

In order to achieve the above object, in the present invention,

Washing the herbal medicines to remove foreign substances, and then folding the medicines to a size of 1 to 12 cm;

Adding to the fermentation tank 7 to 30% by weight of the crude herb medicine, 0.1 to 1% by weight of bacillus subtilis, and 100% by weight of the fermentation broth containing the remainder;

Adding 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin to the fermentation tank ; And

And fermenting the fermentation broth at 23 to 32 ° C for 48 to 72 hours in the fermentation tank to which the mediator is added.

The herbal medicine preferably includes any one of ginseng ginseng, ginseng ginseng, and ginseng.

The method for producing a herbal fermentation broth according to the present invention may further comprise a step of subjecting the fermented herbal fermentation broth to pulverization with stirring, followed by low-temperature ultrasonic extraction at 40 to 60 ° C for 30 to 120 minutes.

In addition, the production method of the present invention may further comprise a step of drying the crude herb medicine so as to have a water content of 10 to 35% by weight after the herb medicine is turned.

Further, in the present invention,

Wherein the medicament for herb medicine fermentation comprises 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin.

Further, in the present invention,

13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin.

Uniformizing the particles by sieving the mixture into a sieve of 100 mesh or less;

Mixing and kneading 10 to 20 parts by weight of water with respect to 100 parts by weight of the mixture;

Drying the dough in the desired size and shape for 24 to 72 hours in the shade; And

And drying the dried dough at 600 to 800 ° C for 3 to 4 hours to obtain a medicament.

The medium may be added in an amount of 2 to 20 parts by weight based on 100 parts by weight of the fermentation broth. In addition, it is preferable that the medium has a size of 0.5 to 2 cm in width and length.

The median of the present invention is produced by mixing 5 kinds of soil, so that it has many pores and richly contains minerals. Therefore, when used in a process of fermenting herbal medicine, it promotes the proliferation of microorganisms and promotes fermentation.

1 is a photograph showing an embodiment of the media of the present invention.
2 is a photograph showing another embodiment of the media of the present invention.
3 is a photograph showing an embodiment in which the media of the present invention is introduced.
4 is a photograph showing an embodiment in which the media of the present invention is inserted.
Fig. 5 is a photograph showing the fermentation state of an experimental group fermentation broth obtained by fermenting a ginseng gan with a median of the present invention.
FIG. 6 is a photograph showing the fermentation state of a control fermentation broth obtained by fermenting a goat ginseng without using the media of the present invention.
Fig. 7 is a photograph showing the fermentation state of another control fermentation broth obtained by fermenting the goat ginseng without using the media of the present invention.
FIG. 8 is a photograph showing the fermentation state of the fermentation broth of an experimental group fermented with water using the media of the present invention. FIG.
FIG. 9 is a photograph showing a fermentation state of a control fermentation broth obtained by fermenting Baechu-ko without using the media of the present invention.
10 is a photograph showing the fermentation state of an experimental fermentation broth obtained by fermenting Gujugi using media of the present invention.
11 is a photograph showing the fermentation state of a control fermentation broth obtained by fermenting Gujugi without using the media of the present invention.

The herbal medicine, which is a raw material in the fermentation of herbal medicine, can be used without limitation for both organisms and dried ones. Wash the herbal medicines to remove foreign substances, and then cut to 1 ~ 12 cm. Preferably, the treatment may be performed after or before the cut so that the final moisture content is about 10 to 35% by weight.

The method for producing a herbal fermentation broth of the present invention can be applied to various herbal medicines. In the present invention, the herbal medicine particularly includes any one of ginseng ginseng, white ginseng, and ginseng.

Bacillus subtilis strain and water are mixed with the prepared raw material medicines as described above to prepare a fermentation broth and put into a fermenter. At this time, it is preferable to add the herb medicine in an amount of 7 to 30% by weight based on the total weight of the fermentation liquid containing the raw material herb medicine, Bacillus subtilis and water. Bacillus subtilis, which is a fermentation strain, is preferably used in an amount of 0.1 to 1% by weight based on the total weight of the fermentation broth. It is more preferable that the Bacillus subtilis is uniformly dispersed in the raw material. The remainder of the fermentation strains and the raw material herbs are filled with water to prepare a fermentation broth of 100% by weight. At this time, preferably 0.5 to 2% by weight of molasses is contained in the fermentation broth.

Separately prepared medicines for herbal fermentation are put into the fermentation tank. 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder, and 9 to 20 parts by weight of kaolinite, kaolinite, zeolite, charcoal powder and kaolin, 15 parts by weight. The feeding of the medium and the fermentation liquid can be done either in the first place or in the order, but also at the same time.

The medium is prepared by mixing white porcelain, yellow earth, zeolite, charcoal powder and kaolin. A mixture of 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin is mixed with a sieve of not more than 100 mesh Filter the particles uniformly, then add water and knead. Water is preferably mixed at a ratio of 10 to 20 parts by weight based on 100 parts by weight of the mixture.

The kneaded mixture is made into a desired size and shape, dried in the shade for 24 to 72 hours, and then aged at 600 to 800 ° C. for 3 to 4 hours to obtain media. The size and shape of the media can be varied as needed. The media may preferably have a size of 0.5 to 2 cm in width and height, respectively. The shape is not limited, and may be, for example, a spherical shape, a cylindrical shape, or the like, but it may have a curved surface to increase its surface irregularity or surface area. As a preferred embodiment, it may have a spherical shape having a diameter of 0.5 to 2 cm, a cylindrical shape having a diameter of 0.5 to 2 cm and a length of 2 to 4 cm.

The mediator of the present invention is used for fermenting herbal medicines and has an effect of promoting fermentation of microorganisms because it has many pores and is rich in minerals.

The media is placed in a ventilated mesh or directly into the fermentor and secured to the bottom. It is preferable that the medium is added in an amount of 2 to 20 parts by weight based on 100 parts by weight of the fermentation broth.

The fermentation preferably proceeds at a temperature of 23 to 32 ° C for 48 to 72 hours, and it is preferable to continuously supply oxygen into the fermentation tank during fermentation.

The herbal fermentation broth fermented by the fermentation process is stirred and pulverized and extracted with low-temperature ultrasonic waves. The low temperature ultrasonic extraction is preferably carried out at a temperature of 40 to 60 DEG C for 30 to 120 minutes.

Extracted herbal medicine extracts can be processed into various herbal medicine products.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples are illustrative of the present invention, and the scope of the present invention is not limited thereto.

< Example  1>

16 parts by weight of white porcelain, 61 parts by weight of loess, 21 parts by weight of zeolite, 9 parts by weight of charcoal powder and 12 parts by weight of kaolin were mixed and sieved with a sieve of 100 mesh. 15 parts by weight of water was mixed with 100 parts by weight of the sieved mixture and kneaded. The kneaded mixture was dried in a shade for 72 hours in a 1-cm-sphere shape, and then was annealed at 750 ° C for 3 hours. The spherical median obtained is shown in Fig.

< Example  2>

15 parts by weight of white porcelain, 60 parts by weight of loess, 20 parts by weight of zeolite, 10 parts by weight of charcoal powder and 13 parts by weight of kaolin were mixed and sieved with a sieve of 100 mesh to homogenize the particles. 13 parts by weight of water was mixed with 100 parts by weight of the sieved mixture and kneaded. The kneaded mixture was made into a cylindrical shape having a diameter of 1 cm, dried in shade for 48 hours, and then baked at 800 ° C for 4 hours. And then cut into 2 ㎝ lengths to obtain a cylindrical medium. The obtained medium of the cylindrical shape is shown in Fig.

< Example  3>

The goat ginseng was selected, and the microbubble aqueous solution was used to remove the foreign substances and then cut into 5 cm size. Reduced goat ginseng was dried to a moisture content of 15%.

To the fermentation tank, 200 g of dried ginseng herb was added and 5 g of Bacillus subtilis strain powder was uniformly sprayed on it. Then, 10 g of molasses was added and water was added to prepare a total of 1000 g of fermentation broth.

40 g of the medium obtained in Example 2 was placed in the mesh and fixed to the bottom of the fermenter. The process of feeding the media into the fermenter is shown in Figures 3 and 4. In FIGS. 3 and 4, the microorganism reaction tank means a reaction tank in which the microbial fermentation reaction will take place, and the settling tank means a reaction tank left in a state where the microbial fermentation reaction is completed. And fermented while supplying oxygen to the aeration unit at a temperature of 28 캜 for 60 hours.

Fermented soybean ginseng fermentation broth was stirred and pulverized and ultrasonically extracted at 50 ℃ for 80 minutes to obtain fermented soybean ginseng extract.

< Example  4>

Example 5 was repeated to obtain a fermented extract of Bacillus subtilis, except that Bacillus subtilis was used in place of the goat ginseng.

< Example  5>

A ginger fermented extract was obtained in the same manner as in Example 3 except that ginger was used instead of ginseng.

< Experimental Example  1>

In order to confirm that the microorganism actively proliferates and promotes fermentation when the mediator of the present invention is used, the following experiment was conducted.

As the experimental group, the fermented extract of the fermented goat ginseng obtained in Example 3 was used. As a control group, fermented soybean ganoderma extract fermented by the same method was used except that the media of the present invention was not used, and two control groups were used. The fermentation broths of two control and experimental groups were cultured in petri dishes using conventional microorganism medium to confirm the degree of microbial growth, and the results are shown in FIGS. 5 to 7. FIG. 5 shows the results of the experimental group, and FIGS. 6 and 7 show the results of the control group.

As can be seen from Fig. 5, microorganism colonies were clearly formed in the experimental group using the media of the present invention. On the other hand, microorganism colonies were not properly formed in the control groups of Figs. 6 and 7.

< Experimental Example  2>

In order to confirm that the microorganism actively proliferates and promotes fermentation when the mediator of the present invention is used, the following experiment was conducted.

As an experimental group, the fermented extract obtained from Example 4 was used. As a control group, a fermented extract of Fermented Phellinus linteus fermented by the same method was used except that the medium of the present invention was not used. The fermentation broth of the experimental group and the control group was cultured in a petri dish using a conventional microorganism medium to confirm the degree of growth of the microorganism. The results are shown in FIGS. 8 and 9.

As can be seen from FIG. 8, in the experimental group using the media of the present invention, microorganism proliferation was active and many colonies were formed, whereas in the control group of FIG. 9, microorganism proliferation was not actively performed and colonies were not formed properly.

< Experimental Example  3>

In order to confirm that the microorganism actively proliferates and promotes fermentation when the mediator of the present invention is used, the following experiment was conducted.

As an experimental group, the fermented Gujusa fermented extract obtained in Example 5 was used. As a control group, the fermented Gujusa fermented extract fermented by the same method was used except that the media of the present invention was not used. The fermentation broth of the experimental group and the control group was cultured in a petri dish using a conventional microorganism medium to confirm the degree of growth of the microorganism. The results are shown in FIGS. 10 and 11.

As can be seen from FIG. 10, in the experimental group using the media of the present invention, microorganism proliferation was active and many colonies were formed, whereas in the control group of FIG. 11, microorganism proliferation was not active and colonies were not formed properly.

Claims (13)

A step of washing the herbal medicine selected from the group consisting of ginseng, wild ginseng, white ginseng, and gugija to remove foreign matter and then folding the ginseng to a size of 1 to 12 cm;
Adding to the fermentation tank 7 to 30% by weight of the crude herb medicine, 0.1 to 1% by weight of bacillus subtilis, and 100% by weight of the fermentation broth containing the remainder;
The medicament for fermentation medicinal herb including 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin is added to the fermentation tank 100 2 to 20 parts by weight per part by weight;
Fermenting the fermentation broth at 23 to 32 ° C for 48 to 72 hours in the fermentation tank into which the medium is introduced; And
Pulverizing the fermented herbal fermentation broth, and subjecting the fermented herbal fermentation broth to low-temperature ultrasonic extraction at 40 to 60 ° C for 30 to 120 minutes. delete delete delete delete delete The method according to claim 1,
Further comprising the step of drying the crude herb medicine so as to have a water content of 10 to 35% by weight. delete The method according to claim 1,
Wherein the medium has a size of 0.5 to 2 cm in width and length, respectively. The method according to claim 1,
13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin.
A step of homogenizing the particles by sieving the mixture with a sieve of not more than 100 mesh;
Mixing and kneading 10 to 20 parts by weight of water with respect to 100 parts by weight of the mixture;
Drying the dough in a desired size and shape for 24 to 72 hours in a shade; And
And drying the dough at 600 to 800 ° C. for 3 to 4 hours to obtain a median. The method of manufacturing a herbal fermentation broth according to claim 1, 13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin,
13 to 20 parts by weight of white porcelain, 53 to 70 parts by weight of loess, 18 to 24 parts by weight of zeolite, 7 to 12 parts by weight of charcoal powder and 9 to 15 parts by weight of kaolin.
Uniformizing the particles by sieving the mixture into a sieve of 100 mesh or less;
Mixing and kneading 10 to 20 parts by weight of water with respect to 100 parts by weight of the mixture;
Drying the dough in the desired size and shape for 24 to 72 hours in the shade; And
And drying the dough at 600 to 800 ° C for 3 to 4 hours to obtain a median. 12. The method of claim 11,
Wherein the medium has a size of 0.5 to 2 cm in width and about 0.5 to 2 cm in length. delete

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