CN107446976A - The preparation method of giant salamander polypeptide powder - Google Patents
The preparation method of giant salamander polypeptide powder Download PDFInfo
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- CN107446976A CN107446976A CN201710604092.8A CN201710604092A CN107446976A CN 107446976 A CN107446976 A CN 107446976A CN 201710604092 A CN201710604092 A CN 201710604092A CN 107446976 A CN107446976 A CN 107446976A
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- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The invention discloses a kind of preparation method of giant salamander polypeptide powder, process in accordance with the following steps：1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughters gall-bladder and adipose tissue；2) clean up, and be cut into cube meat, taking-up drains the water, standby；3) take off and carry out defishying to giant salamander cube meat with ginger juice, xylose, citric acid composite solution, 4) the giant salamander cube meat that deodorant drains is put into 55 60 DEG C of constant temperature baking box and carries out toasting to obtain giant salamander jerky, Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding is sieved, obtains giant salamander digested tankage；5) it is used in the extraction of 6# solvent naphthas and removes fat；6) digest：The complex enzyme zymohydrolysis of elastoser and papain is taken to obtain enzymolysis liquid；7) centrifugation, ultrafiltration obtain giant salamander polypeptide powder liquid；8) it is spray-dried.Giant salamander cuticula can be avoided to the adverse effect caused by polypeptide yield and quality.Pass through baking so that enzymolysis is easier.Digested using the complex enzyme of elastoser and papain composition, improve enzymolysis quality.
Description
Technical field
The present invention relates to a kind of preparation method of giant salamander polypeptide powder, belong to biologically active peptide field.
Background technology
Giant salamander is commonly called as the giant salamander, belongs to Amphibia Caudata Cryptobranchidae, is species more original in existing amphibian.
It is that China is with a long history, there is the evolutionary history up to 3.5 hundred million years, the living fossil that can be compared favourably with giant panda, is that China is traditional
Medicinal animal, early in the medical treasured book of Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》Described in, the medicinal folk prescription of folklore is also a lot of, through the modern times
Analysis on Nutritious Ingredient of Fattening result of study shows：Its is nutritious, and containing 18 kinds of amino acid, composition composition is comprehensive, is that human nutrition needs
The high-quality protein source asked, it is the treasure of " asking it in the four seas ".With the success of artificial breeding, giant salamander is in the important meaning academicly studied
Justice is further elevated, and people, which are constantly recognized the edibility and effect of giant salamander, to deepen continuously.Unfortunately, claim
The deep processed product that giant salamander must be gone up is very few.
The B of CN 103493969 disclose a kind of method for producing giant salamander polypeptide powder, in cube meat by simply pre-processing, adopting
Just directly digested after extracting degreasing with edible oil.
The A of CN 104068204 disclose a kind of preparation method of giant salamander polypeptide powder, and after giant salamander stripping and slicing, infusion is then just straight
Connect enzymolysis.
The A of CN 104206641 also disclose that a kind of giant salamander polypeptide powder and preparation method thereof, also by rejecting
Directly digested after the adipose tissue and gall-bladder of giant salamander.That is, its preparation of the research of current giant salamander polypeptide powder is limited to digest bar
Piece optimization.It has ignored due to the black cuticula that the crust of giant salamander has and processing impacted so that giant salamander was digesting
In journey not thoroughly, enzymolysis process and product are impacted, so as to influence the quality of product.
The content of the invention
In order to solve the above-mentioned technical problem, it is an object of the invention to provide a kind of convenience and efficient giant salamander polypeptide powder
Preparation method, influence of the cuticula to enzymolysis is avoided, improve product quality.
To achieve these goals, technical scheme is as follows：A kind of preparation method of giant salamander polypeptide powder, its feature
It is, processes in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughtered, and remove gall-bladder and adipose tissue；
2) pre-process：Giant salamander is cleaned up, and is cut into cube meat taking-up and drains the water, it is standby；
3) deodorant：Defishying is carried out to giant salamander cube meat with ginger juice, xylose, citric acid composite solution, deodorant temperature is 5-
10℃；
4) toast：The giant salamander cube meat that deodorant drains is put into 55-60 DEG C of constant temperature baking box and carries out toasting to obtain Megalobatrachus japonicus daoidianuas(Blanchard)
It is dry, Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding is sieved, obtains giant salamander digested tankage；
5) degreasing：Giant salamander digested tankage removes fat using the extraction of 6# solvent naphthas, volatilizes solvent and obtains degreasing giant salamander powder；
6) digest：The complex enzyme of elastoser and papain is taken, adds in 45-55 DEG C of hot water and pre-processes, stir evenly
Filtering, is then added in the system of degreasing giant salamander powder and pure water, adjusts pH5.5-6.5 using cushioning liquid, is in temperature
1-2h is digested under the conditions of 40-50 DEG C, obtains enzymolysis liquid；
7) centrifugation, ultrafiltration obtain giant salamander polypeptide powder liquid；
8) it is spray-dried：Giant salamander polypeptide powder liquid is spray-dried, obtains giant salamander polypeptide powder, divides powder to pack giant salamander polypeptide powder
To product.
Using such scheme, giant salamander cube meat first uses ginger juice, xylose, citric acid defishying, in this process, not only
Fishy smell can be removed, moreover it is possible to remove the black cuticula of giant salamander epidermis, reduce black cuticula and enzymolysis is impacted, improve enzymolysis
Product quality.During baking, be advantageous to macromolecular through overbaking again after giant salamander cube meat deodorant and be converted into small molecule, make
It must digest easily.Unlike the prior art, the complex enzyme that the present invention is formed using elastoser and papain is carried out
Enzymolysis, enzymolysis quality is improved, obtained polypeptide color light yellow is partially white (polypeptide made from existing process is yellow), matter
Measure, be advantageous to absorb.By polypeptide filtrate using being spray-dried, the polypeptide powder of 2000-4000 Dalton molecular weight scopes is obtained,
Yield is higher than 30%.
In such scheme：In step 2), giant salamander is cut into 2-3cm giant salamander cube meat.Be advantageous to thorough deodorant, drying.
In such scheme：In step 3), the mass fraction of composite solution ginger juice is 8-10% ginger juice, xylose 1-3%, lemon
Sour 1-3%, the mass ratio of composite solution and giant salamander cube meat is 4-6:1, deodorant time 1-3h.Deodorant, destratum corneum effect are good.
In such scheme：In step 4), 80 mesh sieves are crossed in baking time 3-4h, Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding.
In such scheme：In step 6), the mass ratio of elastoser and papain is 2:1, pretreatment time 5-
15min；
The mass ratio of degreasing giant salamander powder and pure water is 1:2, the addition of complex enzyme is the 0.05%- of degreasing giant salamander powder
0.2%.Hydrolysis result is good.
In such scheme：In step 7), two times of ultrafiltration, the membrane filtration for retaining 2000 Dalton molecular weights is used for the first time,
Take trapped fluid to carry out second of 4000 Dalton molecular weights retention, obtain the giant salamander polypeptide powder of 2000-4000 Dalton molecular weight scopes
Liquid.
Beneficial effect：The preparation method of the giant salamander polypeptide powder of the present invention is simply efficient, can avoid giant salamander cuticula to more
Adverse effect caused by peptide yield and quality.Pass through baking so that enzymolysis is easier.Utilize elastoser and Papain
The complex enzyme of enzyme composition improves to digest and digests quality, the polypeptide powder of the 2000-4000 Dalton molecular weight scopes of preparation, battalion
It is small to support element loss, beneficial to the absorption of human body, available for medical material, medicine, functional food etc., is adapted to industrialized production.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1
A kind of preparation method of giant salamander polypeptide powder, is processed in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughters the position such as gall-bladder and giant salamander peritonaeum, afterbody, four limbs
Adipose tissue, remainder be used for prepare polypeptide.
2) pre-process：Giant salamander is cleaned up, and the giant salamander cube meat for being cut into 2-3cm takes out and drained the water, it is standby.
3) deodorant：Defishying is carried out to giant salamander cube meat with ginger juice, xylose, citric acid composite solution, ginger in composite solution
The mass fraction of juice is 8% ginger juice, and xylose 1%, citric acid 1%, composite solution is 6 with flesh of fish mass ratio:1, deodorant temperature is
5-10 DEG C, deodorant time 3h.
4) toast：The giant salamander cube meat that deodorant drains is put into 55-60 DEG C of constant temperature baking box carry out toast 3-4h obtain greatly
Salamander jerky, 80 mesh sieves are crossed into Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding, obtain giant salamander digested tankage.
5) degreasing：Giant salamander digested tankage removes fat using the extraction of 6# solvent naphthas, volatilizes solvent and obtains degreasing giant salamander powder；
6) digest：Take the quality of the complex enzyme of elastoser and papain, elastoser and papain
Than for 2:1, the amount of complex enzyme is the 0.05% of degreasing giant salamander powder, and complex enzyme is added in 45-55 DEG C of hot water and pre-processes 5min, is stirred
Even filtering, it is then added in the system of degreasing giant salamander powder and pure water, the mass ratio of degreasing giant salamander powder and pure water is 1:2,
In enzymolysis process, pH5.5-6.5 is adjusted using cushioning liquid, 2h is digested under the conditions of being 40-50 DEG C in temperature, obtains enzymolysis liquid.
7) centrifuge, enzymolysis liquid is put into 4000r/min centrifuge centrifuges 10min, take out filtering.
8) two times of ultrafiltration, for the first time using the membrane filtration of 2000 Dalton molecular weights of retention, trapped fluid is taken to carry out second
4000 Dalton molecular weights retain, and obtain the giant salamander polypeptide powder liquid of 2000-4000 Dalton molecular weight scopes.
9) it is spray-dried：Giant salamander polypeptide powder liquid is spray-dried, obtains giant salamander polypeptide powder, to giant salamander polypeptide powder subpackage bag
Dress.
Embodiment 2
A kind of preparation method of giant salamander polypeptide powder, is processed in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughtered, and go to the portion such as gall-bladder and giant salamander peritonaeum, afterbody, four limbs
The adipose tissue of position, remainder are used to prepare polypeptide.
2) pre-process：Giant salamander is cleaned up, and the giant salamander cube meat for being cut into 2-3cm takes out and drained the water, it is standby.
3) deodorant：Defishying is carried out to giant salamander cube meat with ginger juice, xylose, citric acid composite solution, ginger in composite solution
The mass fraction of juice is 10% ginger juice, and xylose 3%, citric acid 3%, composite solution is 4 with giant salamander cube meat mass ratio:1, deodorant temperature
Spend for 5-10 DEG C, deodorant time 1h.
4) toast：The giant salamander cube meat that deodorant drains is put into 55-60 DEG C of constant temperature baking box carry out toast 3-4h obtain greatly
Salamander jerky, 80 mesh sieves are crossed into Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding, obtain giant salamander digested tankage.
5) degreasing：Giant salamander digested tankage removes fat using the extraction of 6# solvent naphthas, volatilizes solvent and obtains degreasing giant salamander powder；
6) digest：Take the quality of the complex enzyme of elastoser and papain, elastoser and papain
Than for 2:1, the addition of complex enzyme is the 0.2% of degreasing giant salamander powder, and complex enzyme is added in 45-55 DEG C of hot water and pre-processed
15min, filtering is stirred evenly, be then added in the system of degreasing giant salamander powder and pure water, the quality of degreasing giant salamander powder and pure water
Than for 1:2, in enzymolysis process, pH5.5-6.5 is adjusted using cushioning liquid, 1h is digested under the conditions of being 40-50 DEG C in temperature, obtains enzyme
Solve liquid.
7) centrifuge, enzymolysis liquid is put into 4000r/min centrifuge centrifuges 10min, take out filtering.
8) two times of ultrafiltration, for the first time using the membrane filtration of 2000 Dalton molecular weights of retention, trapped fluid is taken to carry out second
4000 Dalton molecular weights retain, and obtain the giant salamander polypeptide powder liquid of 2000-4000 Dalton molecular weight scopes.
9) it is spray-dried：Giant salamander polypeptide powder liquid is spray-dried, obtains giant salamander polypeptide powder.
Embodiment 3
A kind of preparation method of giant salamander polypeptide powder, is processed in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughtered, and go to the portion such as gall-bladder and giant salamander peritonaeum, afterbody, four limbs
The adipose tissue of position, remainder are used to prepare polypeptide.
2) pre-process：Giant salamander is cleaned up, and the giant salamander cube meat for being cut into 2-3cm takes out and drained the water, it is standby.
3) deodorant：Defishying is carried out to giant salamander cube meat with ginger juice, xylose, citric acid composite solution, ginger in composite solution
The mass fraction of juice is 10% ginger juice, xylose 2%, citric acid 2%, and the mass ratio of composite solution and giant salamander cube meat is 5:1, deodorant
Temperature is 5-10 DEG C, deodorant time 2h.
4) toast：The giant salamander cube meat that deodorant drains is put into 55-60 DEG C of constant temperature baking box carry out toast 3-4h obtain greatly
Salamander jerky, 80 mesh sieves are crossed into Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding, obtain giant salamander digested tankage.
5) degreasing：Giant salamander digested tankage removes fat using the extraction of 6# solvent naphthas, volatilizes solvent and obtains degreasing giant salamander powder；
6) digest：Take the quality of the complex enzyme of elastoser and papain, elastoser and papain
Than for 2:1, the addition of complex enzyme is the 0.1% of degreasing giant salamander powder, and complex enzyme is added in 45-55 DEG C of hot water and pre-processed
10min, filtering is stirred evenly, be then added in the system of degreasing giant salamander powder and pure water, the quality of degreasing giant salamander powder and pure water
Than for 1:2, in enzymolysis process, pH5.5-6.5 is adjusted using cushioning liquid, 2h is digested under the conditions of being 40-50 DEG C in temperature, obtains enzyme
Solve liquid.
7) centrifuge, enzymolysis liquid is put into 4000r/min centrifuge centrifuges 10min, take out filtering.
8) two times of ultrafiltration, for the first time using the membrane filtration of 2000 Dalton molecular weights of retention, trapped fluid is taken to carry out second
4000 Dalton molecular weights retain, and obtain the giant salamander polypeptide powder liquid of 2000-4000 Dalton molecular weight scopes.
9) it is spray-dried：Giant salamander polypeptide powder liquid is spray-dried, obtains giant salamander polypeptide powder.
The present invention is not limited to above-mentioned specific embodiment, it will be appreciated that one of ordinary skill in the art is without creative
Work can makes many modifications and variations according to the design of the present invention.In a word, all technician in the art are according to this
The design of invention passes through the available technical side of logical analysis, reasoning, or a limited experiment on the basis of existing technology
Case, all should be in the protection domain being defined in the patent claims.
Claims (6)
1. a kind of preparation method of giant salamander polypeptide powder, it is characterised in that process in accordance with the following steps：
1) giant salamander living a couple of days of stopping eating it will be made to empty the intestine, then slaughtered, and remove gall-bladder and adipose tissue；
2) pre-process：Giant salamander is cleaned up, and is cut into cube meat taking-up and drains the water, it is standby；
3) deodorant：Defishying is carried out to giant salamander cube meat with ginger juice, xylose, citric acid composite solution, deodorant temperature is 5-10 DEG C；
4) toast：The giant salamander cube meat that deodorant drains is put into 55-60 DEG C of constant temperature baking box and carries out toasting to obtain giant salamander jerky, will
Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding is sieved, and obtains giant salamander digested tankage；
5) degreasing：Giant salamander digested tankage removes fat using the extraction of 6# solvent naphthas, volatilizes solvent and obtains degreasing giant salamander powder；
6) digest：The complex enzyme of elastoser and papain is taken, adds in 45-55 DEG C of hot water and pre-processes, stir evenly filtering,
It is then added in the system of degreasing giant salamander powder and pure water, pH5.5-6.5 is adjusted using cushioning liquid, is 40-50 in temperature
1-2h is digested under the conditions of DEG C, obtains enzymolysis liquid；
7) centrifugation, ultrafiltration obtain giant salamander polypeptide powder liquid；
8) it is spray-dried：Giant salamander polypeptide powder liquid is spray-dried, obtains giant salamander polypeptide powder.
2. the preparation method of giant salamander polypeptide powder according to claim 1, it is characterised in that：In step 2), giant salamander is cut into 2-
3cm giant salamander cube meat.
3. the preparation method of giant salamander polypeptide powder according to claim 1, it is characterised in that：In step 3), composite solution ginger juice
Mass fraction be 8-10% ginger juice, xylose 1-3%, citric acid 1-3%, composite solution and giant salamander cube meat mass ratio are 4-6:1,
Deodorant time 1-3h.
4. the preparation method of giant salamander polypeptide powder according to claim 1, it is characterised in that：In step 4), baking time 3-4h,
80 mesh sieves are crossed in Megalobatrachus japonicus daoidianuas(Blanchard) dry grinding.
5. according to the preparation method of any one of the claim 1-4 giant salamander polypeptide powders, it is characterised in that：In step 6), elasticity
The mass ratio of protease and papain is 2:1, pretreatment time 5-15min；
The mass ratio of degreasing giant salamander powder and pure water is 1:2, the addition of complex enzyme is the 0.05%-0.2% of degreasing giant salamander powder.
6. the preparation method of giant salamander polypeptide powder according to claim 5, it is characterised in that：In step 7), two times of ultrafiltration, first
The secondary membrane filtration using 2000 Dalton molecular weights of retention, take trapped fluid to carry out second of 4000 Dalton molecular weights retention, obtain
The giant salamander polypeptide powder liquid of 2000-4000 Dalton molecular weight scopes.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110432497A (en) * | 2019-09-03 | 2019-11-12 | 湖北世厚耀榜纪田农业发展有限公司 | A kind of giant salamander protein peptides antialcoholism capsule and preparation method thereof |
| CN110452949A (en) * | 2019-09-23 | 2019-11-15 | 陕西华鲵生物科技有限公司 | A method of preparing Megalobatrachus japonicus daoidianuas(Blanchard) polypeptide powder |
| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
| CN114073754A (en) * | 2020-08-18 | 2022-02-22 | 清华大学 | Giant salamander bone meal extract, preparation method and application thereof |
| CN114098039A (en) * | 2021-11-05 | 2022-03-01 | 暨南大学 | Flavor-enhanced seasoning for Chinese carp as well as preparation method and application of flavor-enhanced seasoning |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110432497A (en) * | 2019-09-03 | 2019-11-12 | 湖北世厚耀榜纪田农业发展有限公司 | A kind of giant salamander protein peptides antialcoholism capsule and preparation method thereof |
| CN110452949A (en) * | 2019-09-23 | 2019-11-15 | 陕西华鲵生物科技有限公司 | A method of preparing Megalobatrachus japonicus daoidianuas(Blanchard) polypeptide powder |
| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
| CN114073754A (en) * | 2020-08-18 | 2022-02-22 | 清华大学 | Giant salamander bone meal extract, preparation method and application thereof |
| CN114098039A (en) * | 2021-11-05 | 2022-03-01 | 暨南大学 | Flavor-enhanced seasoning for Chinese carp as well as preparation method and application of flavor-enhanced seasoning |
| CN114098039B (en) * | 2021-11-05 | 2023-09-15 | 暨南大学 | Flavor-enhanced geofish seasoning as well as preparation method and application thereof |
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Application publication date: 20171208 |