CN104206641A - Megalobatrachus protein polypeptide powder and preparation method thereof - Google Patents
Megalobatrachus protein polypeptide powder and preparation method thereof Download PDFInfo
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- CN104206641A CN104206641A CN201410420731.1A CN201410420731A CN104206641A CN 104206641 A CN104206641 A CN 104206641A CN 201410420731 A CN201410420731 A CN 201410420731A CN 104206641 A CN104206641 A CN 104206641A
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- 229920001184 polypeptide Polymers 0.000 title claims abstract description 43
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 43
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 43
- 239000000843 powder Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 title abstract description 8
- 108090000623 proteins and genes Proteins 0.000 title abstract description 8
- 235000013372 meat Nutrition 0.000 claims abstract description 44
- 239000004365 Protease Substances 0.000 claims abstract description 28
- 108091005804 Peptidases Proteins 0.000 claims abstract description 18
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 150000001875 compounds Chemical class 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229960002635 potassium citrate Drugs 0.000 claims abstract description 8
- 239000001508 potassium citrate Substances 0.000 claims abstract description 8
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims abstract description 8
- 235000011082 potassium citrates Nutrition 0.000 claims abstract description 8
- 239000000796 flavoring agent Substances 0.000 claims abstract description 7
- 235000019634 flavors Nutrition 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 26
- 229940088598 enzyme Drugs 0.000 claims description 26
- 235000019419 proteases Nutrition 0.000 claims description 16
- 108090000526 Papain Proteins 0.000 claims description 10
- 235000019834 papain Nutrition 0.000 claims description 10
- 229940055729 papain Drugs 0.000 claims description 10
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 210000000577 adipose tissue Anatomy 0.000 claims description 6
- 210000000232 gallbladder Anatomy 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 238000001976 enzyme digestion Methods 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 238000005374 membrane filtration Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 5
- 238000009826 distribution Methods 0.000 claims description 2
- 241000157862 Dicamptodontidae Species 0.000 claims 18
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- 238000000034 method Methods 0.000 abstract description 4
- 238000004108 freeze drying Methods 0.000 abstract description 2
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- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
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- 238000005516 engineering process Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
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- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
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- 229940111202 pepsin Drugs 0.000 description 2
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- 239000012190 activator Substances 0.000 description 1
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- 238000009395 breeding Methods 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- 229910052737 gold Inorganic materials 0.000 description 1
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- 230000002085 persistent effect Effects 0.000 description 1
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Abstract
The invention relates to a megalobatrachus protein polypeptide powder and a preparation method thereof. The preparation method comprises the following steps of mixing megalobatrachus meat paste and water according to a solid-liquid ratio of 1:2-1:5; adding potassium citrate accounting for 0.1 percent-0.55 percent of the mass of the meat paste, and neutral protease or flavor protease accounting for 0.1 percent -1 percent of the mass of the meat paste for enzymolysis, wherein enzymolysis is performed at a pH value of 6.7-7.5 and at temperature of 40-60 DEG C for 1-3 h; adding a compound protease accounting for 0.1 percent-5 percent of the mass of the megalobatrachus meat paste and maintaining the temperature at 40-60 DEG C for enzymolysis for 2-3h; and after enzymolysis, heating the product to 100 DEG C, maintaining the temperature for 10-15 min, filtering the product to obtain protein polypeptide hydrolysate, adding activated carbon accounting for 0.1 percent-3 percent of the mass of the megalobatrachus meat paste, filtering the product to obtain protein polypeptide hydrolysate, and subjecting the hydrolysate to vacuum concentration under reduced pressure and freeze drying in vacuum to obtain the megalobatrachus protein polypeptide powder. The megalobatrachus protein polypeptide powder prepared through the method provided in the invention has high utilization and a good taste, and can be easily absorbed by a human body. The hydrolytic amino acid content of the megalobatrachus protein polypeptide powder can be up to 98 percent.
Description
Technical field
The present invention relates to a kind of giant salamander polypeptide powder and preparation method thereof, specifically a kind of is primary raw material with Megalobatrachus japonicus daoidianuas(Blanchard), and giant salamander polypeptide powder obtained by enzymolysis and preparation method thereof, belongs to edible protein polypeptide manufacture field.
Background technology
Giant salamander is commonly called as the giant salamander, is amphibian animal maximum in the world.How on the books in China's successive dynasties Chinese herbal medicine secret recipe, in the pharmacopeia such as Compendium of Material Medica in ancient times, Collective Notes to the Canon of Materia medica and supplement to the Herbal, giant salamander is had to the description of the medicinal efficacies such as " silly disease is controlled in squama order, enriching yin and nourishing kidney, promoting the circulation of qi of enriching blood " and " control silly disease, control persistent ailment ".
Detect through science: containing 17 seed amino acids in Megalobatrachus japonicus daoidianuas(Blanchard), wherein 8 kinds of essential amino acids, in 100g Megalobatrachus japonicus daoidianuas(Blanchard) dry, amino acid content is up to 91.92g, its essential amino acids content is 36.69%, be containing one of the abundantest, the most comprehensive species of amino acid, thus the meat of giant salamander is also one of protein resource pressing close to human body needs most.Pure white fresh and tender, fertile and oiliness, the flat flavor of giant salamander meat and delicious, nutritious, is described as " in water living person's ginseng " and " soft gold ", have in Southeast Asia " eat a salamander meat grow one the cycle of sixty years power " say, be a kind of high-grade nourishing health treasure.
Since entering 21 century, due to the breakthrough of giant salamander culture and raising technology, vigorously supporting of national policy and applying of special cultivation technology, whole nation giant salamander culture scale development grows, and according to incompletely statistics, present national giant salamander controllable resources reaches more than 5,000,000 tails.But so far, for the exploitation of giant salamander, be also just in the original material use stage, such as cook giant salamander dish, make giant salamander profile handicraft etc., the exploitation of giant salamander profound level seldom or almost do not have.Along with giant salamander culture technology is more and more ripe, resource constantly accumulates, and existing application market can not meet the sale needs expanding breeding scale.At present, giant salamander deep processed products such as commercially having beauty treatment, keep healthy, be medicinal is sold.Giant salamander polypeptide powder and preparation method thereof is related documents report also, but existing method exists, and polypeptide recovery utilization rate is on the low side, the mouthfeel that manufactures a finished product is not good (slightly bitter taste), purity is not high, fat content crosses the problems such as high.
Summary of the invention
In order to overcome in existing giant salamander polypeptide powder production technology, polypeptide recovery utilization rate is on the low side, the mouthfeel that manufactures a finished product is not good, purity is not high, fat content crosses the problems such as high, the invention provides a kind of giant salamander polypeptide powder and preparation method thereof, it utilizes the efficient combination of exogenous enzymes and dosage to add, reach the effect of enzymolysis, process through follow-up purifying, giant salamander polypeptide powder utilization rate is improved, and mouthfeel is better, is conducive to absorption of human body.
The giant salamander polypeptide powder that the present invention obtains, its molecular weight distribution is: molecular weight is 1 Da ~ 200Da, and its content is 0.1% ~ 5%; Molecular weight is 201Da ~ 1000 Da, and its content is 50% ~ 75%; Molecular weight is 1001Da ~ 10000 Da, and its content is 9.9% ~ 40%; Molecular weight is greater than 10000 Da, and its content is 5% ~ 15%.
Its preparation method comprises the following steps:
(1) the giant salamander bloodletting of butchering fresh, clean, the adipose tissue at the positions such as giant salamander peritonaeum, afterbody, four limbs and gall-bladder are rejected, then putting into mixer is cut into meat gruel, and mixing time controls at 5 ~ 10min;
(2) giant salamander meat gruel is mixed with the ratio of solid-to-liquid ratio 1:2 ~ 1:5 with water, and adopt vortex mixer that solid-liquid is fully mixed, after 40 mesh sieves, put into enzyme digestion reaction still;
(3) add the potassium citrate of giant salamander meat gruel quality 0.1% ~ 0.55%, add neutral proteinase or the flavor protease of giant salamander meat gruel quality 0.1% ~ 1%, regulate pH be 6.5 ~ 7.5, holding temperature at 40 ~ 60 DEG C, enzymolysis 1 ~ 3 h; Then add the compound protease of giant salamander meat gruel quality 0.1% ~ 5%, holding temperature, at 40 ~ 60 DEG C, continues enzymolysis 2 ~ 3h;
(4) after enzymolysis terminates, be heated to 100 DEG C, maintain 10 ~ 15min, the enzyme that goes out, sterilization;
(5), after centrifugal or plate-frame filtering, the clear liquid of acquisition is carried out membrane filtration again, and filter membrane specification is 0.05 μm-5 μm, obtains polypeptide hydrolyzate;
(6) in gained polypeptide hydrolyzate, add the active carbon of giant salamander meat gruel quality 0.1% ~ 3%, control temperature is 55 ~ 65 DEG C, adopts mixing plant to stir 2 ~ 3h, filter-cloth filtering;
(7) (6) gained polypeptide hydrolyzate reduced vacuum at 55 ~ 65 DEG C is concentrated, when being 20% ~ 30% to solid content;
(8) at temperature-25 ~-40 DEG C, vacuum freeze drying about 4 ~ 16h, obtains giant salamander polypeptide powder.After testing, its hydrolysising amino acid content is up to 98%.
In step (2), the solid-to-liquid ratio of giant salamander meat gruel and water is 1:2, and the addition of potassium citrate is 0.1% ~ 0.25% of giant salamander meat gruel weight.
In step (3), the addition of neutral proteinase or flavor protease is 0.2% ~ 0.5% of giant salamander meat gruel quality, the addition of compound protease is 0.2% ~ 1% of giant salamander meat gruel quality, and compound protease is that 1:1 forms by papain and trypsase according to mass ratio.
In step (6), the addition of active carbon is 0.1% ~ 0.3% of giant salamander meat gruel quality.
The present invention is cut by the giant salamander fresh government official being cleaned removal adipose tissue and gall-bladder and mixes meat gruel, and meat gruel is mixed with water, add appropriate potassium ion as activator, first carry out endogenous enzymes hydrolysis, and then add exogenous enzymes raising proteolysis and amino acid converting rate, to keep mouthfeel.After through a series of enzyme that goes out, remove slag, degreasing, purifying (decolouring except raw meat), concentrated, freeze drying process, obtained giant salamander polypeptide powder.Finished product prepared by the present invention is with slight albumin milk fragrance, Powdered, dry exquisiteness, milky, good, the easy absorption of rehydration, lipidated protein high (more than 90%), fat content low (less than 2%), moisture are low, the features such as easy preservation, obtained finished product is a splendid protide supplement.
Detailed description of the invention
Embodiment 1:
A kind of giant salamander polypeptide powder and preparation method thereof, concrete preparation process is:
(1) the giant salamander bloodletting of butchering fresh, clean, the adipose tissue at the positions such as giant salamander peritonaeum, afterbody, four limbs and gall-bladder are rejected, then putting into mixer is cut into meat gruel, and mixing time controls at 5min;
(2) take giant salamander meat gruel 20kg, water 40kg, adopt vortex mixer that solid-liquid is fully mixed, cross 40 mesh sieves, put into enzyme digestion reaction still.
(3) add the potassium citrate of 20g respectively, 20g neutral proteinase, regulate pH be 6.5, holding temperature at 40 DEG C, enzymolysis 1 h; Then add the compound protease (being made up of papain 10g and trypsase 10g) of 20g, holding temperature, at 55 DEG C, continues enzymolysis 2h.
(4) after enzymolysis terminates, be heated to 100 DEG C, maintain 10min, the enzyme that goes out, sterilization;
(5), after centrifugal or plate-frame filtering, the clear liquid of acquisition is carried out membrane filtration again, and filter membrane specification is 0.05 μm, obtains polypeptide hydrolyzate;
(6) in gained polypeptide hydrolyzate, add the active carbon of 20g, control temperature is 55 DEG C, adopts mixing plant to stir 2h, filter-cloth filtering;
(7) (6) gained polypeptide hydrolyzate reduced vacuum at 55 DEG C is concentrated, when being 20% to solid content;
(8) at temperature-25 DEG C, vacuum freeze drying is about 4h, obtains giant salamander polypeptide powder.After testing, its hydrolysising amino acid content is up to 98%.
Embodiment 2:
(1) the giant salamander bloodletting of butchering fresh, clean, the adipose tissue at the positions such as giant salamander peritonaeum, afterbody, four limbs and gall-bladder are rejected, then putting into mixer is cut into meat gruel, and mixing time controls at 8min;
(2) take giant salamander meat gruel 30kg, water 60kg, adopt vortex mixer that solid-liquid is fully mixed, cross 40 mesh sieves, put into enzyme digestion reaction still.
(3) add the potassium citrate of 30g, add the flavor protease of 30g, regulate pH be 7, holding temperature at 50 DEG C, enzymolysis 2 h; Then add the compound protease (being made up of papain 15g and trypsase 15g) of 30g, holding temperature, at 50 DEG C, continues enzymolysis 2.5h.
(4) after enzymolysis terminates, be heated to 100 DEG C, maintain 15min, the enzyme that goes out, sterilization;
(5), after centrifugal or plate-frame filtering, the clear liquid of acquisition is carried out membrane filtration again, and filter membrane specification is 1.25 μm, obtains polypeptide hydrolyzate;
(6) in gained polypeptide hydrolyzate, add the active carbon of 30g, control temperature is 60 DEG C, adopts mixing plant to stir 2.5h, filter-cloth filtering;
(7) (6) gained polypeptide hydrolyzate reduced vacuum at 60 DEG C is concentrated, when being 25% to solid content;
(8) at temperature-35 DEG C, vacuum freeze drying is about 10h, obtains giant salamander polypeptide powder.After testing, its hydrolysising amino acid content is up to 98.5%.
Embodiment 3:
(1) the giant salamander bloodletting of butchering fresh, clean, the adipose tissue at the positions such as giant salamander peritonaeum, afterbody, four limbs and gall-bladder are rejected, then putting into mixer is cut into meat gruel, and mixing time controls at 10min;
(2) take giant salamander meat gruel 40kg, water 80kg, adopt vortex mixer that solid-liquid is fully mixed, cross 40 mesh sieves, put into enzyme digestion reaction still.
(3) add the potassium citrate of 40g, add 40g neutral proteinase, regulate pH be 6.5, holding temperature at 55 DEG C, enzymolysis 2h; Then add the compound protease (being made up of papain 6g and trypsase 6g) of 12g, holding temperature, at 55 DEG C, continues enzymolysis 3h.
(4) after enzymolysis terminates, be heated to 100 DEG C, maintain 15min, the enzyme that goes out, sterilization;
(5), after centrifugal or plate-frame filtering, the clear liquid of acquisition is carried out membrane filtration again, and filter membrane specification is 5 μm, obtains polypeptide hydrolyzate;
(6) in gained polypeptide hydrolyzate, add the active carbon of 40g, control temperature is 65 DEG C, adopts mixing plant to stir 3h, filter-cloth filtering;
(7) (6) gained polypeptide hydrolyzate reduced vacuum at 65 DEG C is concentrated, when being 30% to solid content;
(8) at temperature-40 DEG C, vacuum freeze drying is about 16h, obtains giant salamander polypeptide powder.After testing, its hydrolysising amino acid content is up to 99%.
About the optimum organization of exogenous enzymes and the selection of environment, the present inventor, through too much organizing contrast test, selects with the final combination and action condition that confirm exogenous enzymes.
1, by under identical temperature conditions (55 DEG C), single enzymolysis is carried out to the giant salamander meat gruel of same dose, draws the most applicable pH value environment that different protease is hydrolyzed giant salamander meat gruel enzymeization.
As can be seen from above table, the best enzyme pH condition of trypsase and papain relatively, utilizes the compound protease be made up of trypsase and papain the most suitable in pH6.5-7 Water Under solution.。
2, choose pepsin and papain (complex enzyme A), trypsase and papain (complex enzyme B), pepsin and trypsase (complex enzyme C) and carry out two enzyme combination, carry out orthogonal experiment for the combination using dosage of enzyme, the solid-liquid ratio of giant salamander meat gruel, hydrolysis time, pH value, draw the best of breed enzyme that giant salamander meat gruel enzymeization is hydrolyzed and condition.(in combination enzyme, often kind of enzyme amount is got equal)
As can be seen from above table, enzymeization for giant salamander meat gruel is hydrolyzed, use complex enzyme B will save on using dosage than other two kinds combination enzymes, action time is relatively short simultaneously, and best enzyme pH and endogenous enzymes is hydrolyzed and neutral proteinase, papain action pH close, the optimum enzyme that can be used as the hydrolysis of giant salamander meat gruel enzymeization is considered.
3, by the different action times to identical enzyme, operative temperature and agitating mode (mixing speed) experimental contrast analysis, optimum combination data are drawn.
Visible embodiment 3 is preferred version.
Claims (5)
1. a giant salamander polypeptide powder, is characterized in that, its molecular weight distribution is: molecular weight is 1 Da ~ 200Da, and its content is 0.1% ~ 5%; Molecular weight is 201Da ~ 1000 Da, and its content is 50% ~ 75%; Molecular weight is 1001Da ~ 10000 Da, and its content is 9.9% ~ 40%; Molecular weight is greater than 10000 Da, and its content is 5% ~ 15%.
2. a preparation method for giant salamander polypeptide powder, is characterized in that, comprises the following steps:
(1) the giant salamander bloodletting of butchering fresh, clean, the adipose tissue at the positions such as giant salamander peritonaeum, afterbody, four limbs and gall-bladder are rejected, then putting into mixer is cut into meat gruel, and mixing time controls at 5 ~ 10min;
(2) giant salamander meat gruel is mixed with the ratio of solid-to-liquid ratio 1:2 ~ 1:5 with water, and adopt vortex mixer that solid-liquid is fully mixed, after 40 mesh sieves, put into enzyme digestion reaction still;
(3) add the potassium citrate of giant salamander meat gruel quality 0.1% ~ 0.55%, add neutral proteinase or the flavor protease of giant salamander meat gruel quality 0.1% ~ 1%, regulate pH be 6.5 ~ 7.5, holding temperature at 40 ~ 60 DEG C, enzymolysis 1 ~ 3 h; Then add the compound protease of giant salamander meat gruel quality 0.1% ~ 5%, holding temperature, at 40 ~ 60 DEG C, continues enzymolysis 2 ~ 3h;
(4) after enzymolysis terminates, be heated to 100 DEG C, maintain 10 ~ 15min, the enzyme that goes out, sterilization;
(5), after centrifugal or plate-frame filtering, the clear liquid of acquisition is carried out membrane filtration again, and filter membrane specification is 0.05 μm-5 μm, obtains polypeptide hydrolyzate;
(6) in gained polypeptide hydrolyzate, add the active carbon of giant salamander meat gruel quality 0.1% ~ 3%, control temperature is 55 ~ 65 DEG C, adopts mixing plant to stir 2 ~ 3h, filter-cloth filtering;
(7) (6) gained polypeptide hydrolyzate reduced vacuum at 55 ~ 65 DEG C is concentrated, when being 20% ~ 30% to solid content;
(8) at temperature-25 ~-40 DEG C, vacuum freeze drying about 4 ~ 16h, obtains giant salamander polypeptide powder.
3. the preparation method of giant salamander polypeptide powder according to claim 2, is characterized in that, in step (2), the solid-to-liquid ratio of giant salamander meat gruel and water is 1:2, and the addition of potassium citrate is 0.1% ~ 0.25% of giant salamander meat gruel weight.
4. the preparation method of giant salamander polypeptide powder according to claim 2, it is characterized in that, in step (3), the addition of neutral proteinase or flavor protease is 0.2% ~ 0.5% of giant salamander meat gruel quality, the addition of compound protease is 0.2% ~ 1% of giant salamander meat gruel quality, and compound protease is that 1:1 forms by papain and trypsase according to mass ratio.
5. the preparation method of giant salamander polypeptide powder according to claim 2, is characterized in that, in step (6), the addition of active carbon is 0.1% ~ 0.3% of giant salamander meat gruel quality.
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| CN104982970A (en) * | 2015-08-06 | 2015-10-21 | 重庆馗丰食品有限公司 | Making method and product of pure giant salamander meat meal |
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| CN105962380A (en) * | 2016-06-15 | 2016-09-28 | 通江县光泰科技发展有限责任公司 | Giant salamander whole-body albumen powder and preparing method thereof |
| CN108835185A (en) * | 2018-09-10 | 2018-11-20 | 张家界金鲵生物工程股份有限公司 | A kind of production method and products thereof of the coarse cereal biscuit of protein peptides containing giant salamander |
| CN109943616A (en) * | 2019-04-18 | 2019-06-28 | 威海市宇王集团海洋生物工程有限公司 | A method of the abstraction function albumen from giant salamander |
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| CN104982970A (en) * | 2015-08-06 | 2015-10-21 | 重庆馗丰食品有限公司 | Making method and product of pure giant salamander meat meal |
| CN105399815A (en) * | 2015-10-12 | 2016-03-16 | 成都智企联盟企业管理有限公司 | Andrias davidianus collagen glycopeptide and application thereof |
| CN105399815B (en) * | 2015-10-12 | 2019-01-18 | 张家界金驰天问农业科技有限公司 | Giant salamander collagen sugar peptide and application thereof |
| CN105962380A (en) * | 2016-06-15 | 2016-09-28 | 通江县光泰科技发展有限责任公司 | Giant salamander whole-body albumen powder and preparing method thereof |
| CN108835185A (en) * | 2018-09-10 | 2018-11-20 | 张家界金鲵生物工程股份有限公司 | A kind of production method and products thereof of the coarse cereal biscuit of protein peptides containing giant salamander |
| CN109943616A (en) * | 2019-04-18 | 2019-06-28 | 威海市宇王集团海洋生物工程有限公司 | A method of the abstraction function albumen from giant salamander |
| CN110584128A (en) * | 2019-10-18 | 2019-12-20 | 成都鲵肽生物科技有限公司 | Giant salamander polypeptide powder composition for postpartum recovery |
| CN110584129A (en) * | 2019-10-18 | 2019-12-20 | 成都鲵肽生物科技有限公司 | Giant salamander polypeptide powder composition for tumor rehabilitation |
| CN110754667A (en) * | 2019-10-18 | 2020-02-07 | 成都鲵肽生物科技有限公司 | Giant salamander polypeptide powder composition for immune regulation |
| CN110628554A (en) * | 2019-11-04 | 2019-12-31 | 茅台学院 | Antioxidant giant salamander peptide sauce-flavor wine and preparation method thereof |
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