CN109943616A - A method of the abstraction function albumen from giant salamander - Google Patents
A method of the abstraction function albumen from giant salamander Download PDFInfo
- Publication number
- CN109943616A CN109943616A CN201910313221.7A CN201910313221A CN109943616A CN 109943616 A CN109943616 A CN 109943616A CN 201910313221 A CN201910313221 A CN 201910313221A CN 109943616 A CN109943616 A CN 109943616A
- Authority
- CN
- China
- Prior art keywords
- giant salamander
- enzyme preparation
- enzyme
- weight
- mentioned steps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to technical field of bioengineering, the method of specially a kind of abstraction function albumen from giant salamander, it is comprising steps of pre-treatment, soda acid processing, hot water extraction, liquid phase separation, enzymatic hydrolysis, filtering, reduced pressure and centrifugal spray drying, by carrying out cleaning to giant salamander and height crushes the Giant salamander bone meat gruel obtained, alkali process is recycled to remove the lipid in giant salamander skin and flesh the digestion such as calcium salt in Giant salamander bone first with acid processing.Giant salamander is different to the stability of heat using protein peptides and non-peptide substances after pre-treatment, carries out high temperature extraction to giant salamander bone and flesh.Extracting solution passes through shearing of the different enzymatic reagents to peptide, obtains uniform enzyme shearing liquid.Subsequently through the thickening filtration for carrying out different permeable membranes to enzyme shearing liquid, obtain the small peptide of molecular weight in a certain range, and the peptide of the molecular weight ranges accounts for 85% of peptide or more, can greatly increase absorption efficiency, provides good basis to peptide product effect Journal of Sex Research of different molecular weight ranges.
Description
Technical field
The present invention relates to technical field of bioengineering, the method for specially a kind of abstraction function albumen from giant salamander.
Background technique
Functional protein is the native biopolymer of zooblast synthesis, if collagen is as the weight in extracellular matrix
Want ingredient be almost present in it is organized in, account for about the 30% of animal total protein, there is high resistance to extend ability, make for collagen
The connective tissues such as skin are maintained certain structure, realize and support and protect cell, tissue and body function, to cell and group
The structure knitted and performance etc. are very crucial.Collagen peptide be using collagen as raw material using acid/base processing, enzymic degradation or
In conjunction with the mixtures of polypeptides that multiple form obtains, main component is the inhomogenous polypeptide of the molecular weight that is formed after collagen degradation.
Chinese giant salamander, Andrias davidianus is commonly called as the giant salamander, for the rare precious amphibian in the whole world, China wildlife under second class protection, generation
Boundary's first class of protection endangered species.China giant salamander be mainly distributed on the Changjiang river in China, the Yellow River, Pearl River Delta middle and upper reaches tributary in,
Especially in the majority with provinces and cities such as Sichuan, Hubei, Hunan, Chongqing.National giant salamander population quantity has been added at present less than 500,000 tails
It is more than 3000000 tails.National industrial policies are being kept reforming, and are also being continued to increase to the support dynamics of giant salamander culture.Nowadays giant salamander
Efficiently cultivation is with industry development using among carrying forward vigorously.Giant salamander culture industry has been developed more than 30 years, with reproductive number
It is continuously increased, the proliferative amount of wild resource is also increasing, also gradually flourishing to the intensive processing of giant salamander.
Modern medicine is pointed out: Megalobatrachus japonicus daoidianuas(Blanchard) is delicate, and delicious flavour contains good protein and amino acid abundant, micro member
Element.Often it is edible can with clever intelligence development, anti-aging, improve hematopoiesis and immune function, to prevent cardiovascular and cerebrovascular disease, anaemia and
Tumour has certain effect.The mucus of its skin secretion contains there are many " bombesin ", has certain antibacterial action, can be used as new
" antibiotic " medicament sources researched and developed.Skin, muscle, internal organs, bone and its secretion of giant salamander can be used as medicine, greatly
The specific drug that the fat of salamander contains to be treatment scald, burn there are many unsaturated fatty acid.Technical research industry development is because it is special
Some medical values, domestic many pharmacy corporations have started the upsurge of one development and utilization giant salamander now, mainly there is medicament for treating scald
Object, health care product, cosmetics and skincare product etc..
Giant salamander skin contains 61.3% collagen, and collagen is a kind of containing glycoprotein, it belongs to insoluble fine type egg
White matter, the elasticity that can be repaired tissue, keep skin and bone, keeps skin lubrication, bone tough；Sulphur containing metal in giant salamander body
Albumen (MT) can remove people's interior free yl and heavy metal ion, prevent heavy metal poisoning；The mucus of epidermis secretion contains activity gel
Former albumen, can beauty and prevention senile dementia and anti-curing cancers.
Summary of the invention
It is an object of the invention to solve the problems mentioned above in the background art, giant salamander can be comprehensively utilized, be mentioned
The resource utilization of high giant salamander increases the added value of giant salamander.
To achieve the above object, the invention provides the following technical scheme: a kind of method of the abstraction function albumen from giant salamander,
The method follows the steps below:
After step S1, removal giant salamander internal organ and four limbs, blood are put only, stripping and slicing is carried out, then Giant salamander bone meat piece head is put into skeleton breaking machine
In, obtain Giant salamander bone meat gruel；
Giant salamander bone meat gruel in above-mentioned steps S1 is put into deliming tank by step S2, after pure water cleaning is added, adds giant salamander weight
Hydrochloric acid carries out deliming processing after 2 times of dilution of amount, and time 0.5h is disposed and pure water cleaning is added twice, then with 1% mass
It is 10.0 that the food grade sodium hydroxide of score, which is neutralized, adjusts pH value, is washed with water after alkali process 0.5h, until pH value is washed till
7.0~8.0；
Material in above-mentioned steps S2 is gone to extractor from deliming tank by step S3, and 3~4 times that giant salamander bone and flesh weight is added are pure
Water is warming up to 95 DEG C, persistently extracts 3h, persistently stirred with agitating paddle in the process；
Step S4 closes agitating paddle after the completion of above-mentioned steps S3 extraction, stops stirring, and tank to be extracted is down to room temperature, stands
12h waits being layered, and upper layer grease is centrifugated, extracting solution is obtained；
Extracting solution in above-mentioned steps S4 is put into enzymatic vessel, is warming up to 55~65 DEG C, is adjusted to the optimum response of enzyme by step S5
Condition is warming up to 95 DEG C according still further to the first enzyme preparation, the second enzyme preparation, third enzyme preparation is sequentially sequentially added after reaction,
Keep 30min that enzyme-deactivating is obtained enzymolysis liquid；
Gained enzymolysis liquid in above-mentioned steps S5 is cooled to 50~60 DEG C, deodorization processing is carried out, by flame filter press by step S6
It is precoated with diatomite, 0.5h is mixed in 2~4% active carbon that giant salamander weight is added in enzymolysis liquid, and repressurization is passed through
Flame filter press, until filtrate is in yellow clear liquid；
Yellow clear liquid obtained in above-mentioned steps S6 is carried out one using permeable membranes such as composite tube type films by step S7
Determine solute and the solvent separation of molecular weight；
Solvent after separating in above-mentioned steps S7 is used nanofiltration membrane to carry out 150~1000Dal small organic molecule by step S8 again
Separation；
Step S9 is concentrated to solvable by the filtrate isolated in above-mentioned steps S8 using dual-effect concentrator decompression heating to filtrate
Property solid content up to 40~60% when carry out centrifugal spray drying, albumen powder is made.
As a currently preferred scheme, the first enzyme preparation and the second enzyme preparation in the step S5 are in albumen
Enzyme cutting, third enzyme preparation are albumen excision enzyme.
As a currently preferred scheme, the first enzyme preparation weight be giant salamander bone and flesh weight 0.15~
0.25%, the second enzyme weight of formulation is the 0.15~0.25% of giant salamander bone and flesh weight, and the third enzyme preparation weight is giant salamander
The 0.04~0.06% of bone and flesh weight.
It is added after the first enzyme preparation is added into enzymatic vessel and stirs 15min as a currently preferred scheme
Second enzyme preparation carries out shearing 3h, adds third enzyme preparation and carries out shearing 2h.
As a currently preferred scheme, first enzyme preparation is neutral proteinase, and second enzyme preparation is
Alkali protease, the third enzyme preparation are de- bitter protease.
As a currently preferred scheme, the control inlet temperature of centrifugal spray drying described in the step S9 is
160~190 DEG C, air outlet temperature is 90~100 DEG C and feed rate is 100~300r/min.
It into film pressure is 1.6~1.7/Bar in the step S7 as a currently preferred scheme, film pressure out
For 0.8~1.0 Bar, dope flow is 300~400LPM, and clear liquid flow is 70~90LPM.
It into film pressure is 10~20Bar in the step S8, film pressure is 10 out as a currently preferred scheme
~20Bar, starting at first 5 minutes dope flow is 20~40LPM, clear liquid flow > 100LPM, and filter pressure is
0.35MPA。
As a currently preferred scheme, hydrochloric acid after being diluted in the step S2, concentrated hydrochloric acid: water=1:3.
The beneficial effects of the present invention are: by carrying out the Giant salamander bone meat gruel that cleaning and height crushing obtain to giant salamander, first
Alkali process is recycled to remove the lipid in giant salamander skin and flesh the digestion such as calcium salt in Giant salamander bone using acid processing.Before giant salamander passes through
It is different to the stability of heat using protein peptides and non-peptide substances after processing, high temperature extraction is carried out to giant salamander bone and flesh.Extracting solution warp
Shearing of the different enzymatic reagents to peptide is crossed, uniform enzyme shearing liquid is obtained.Different permeable membranes are carried out subsequently through to enzyme shearing liquid
Thickening filtration obtains the small peptide of molecular weight in a certain range, and the peptide of the molecular weight ranges accounts for 85% of peptide or more, Ke Yi great
It is big to increase absorption efficiency, provide good basis to peptide product effect Journal of Sex Research of different molecular weight ranges；Improve the resource of giant salamander
Utilization rate increases the added value of giant salamander.
Detailed description of the invention
Fig. 1 is process flow chart of the invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
The present invention provides a kind of method of abstraction function albumen from giant salamander, and the method follows the steps below:
Step S1, pre-treatment: removal giant salamander internal organ and four limbs, blood put it is net after, carry out stripping and slicing, then Giant salamander bone meat piece head is put into
In skeleton breaking machine, Giant salamander bone meat gruel is obtained, 1kg is weighed；
Step S2, soda acid processing: putting into deliming tank for the Giant salamander bone meat gruel in above-mentioned steps S1, after pure water cleaning is added, then
The processing of 2kg food grade hydrochloric acid (concentration is 10% or so after dilution) is added, time 0.5h is disposed and pure water cleaning two is added
Secondary, it is 10.0 that the food grade sodium hydroxide for adding 1% mass fraction, which is neutralized, adjusts pH value, is stirred while adding, often
10min measurement is primary, until pH value is identical twice, pH value washes with water after stablizing 0.5h, until pH value is washed till 7.0；
Step S3, hot water extract: the material in above-mentioned steps S2 being gone to extractor from deliming tank, the 3 of giant salamander bone and flesh weight are added
Times pure water, is warming up to 95 DEG C, persistently extracts 3h, persistently stirred with agitating paddle in the process；
Liquid phase separation: step S4 after the completion of above-mentioned steps S3 extraction, stops heating, stops stirring, tank to be extracted is down to room
Temperature stands 12h and waits being layered, upper layer grease is centrifugated, and oil reservoir centrifugation is excluded to obtain extracting solution using centrifuge；
Step S5, enzymatic hydrolysis: putting into enzymatic vessel for the extracting solution in above-mentioned steps S4, be warming up to 55 DEG C, is adjusted to the best anti-of enzyme
Condition is answered, according still further to the first enzyme preparation, the second enzyme preparation, third enzyme preparation is sequentially sequentially added, is warming up to 95 after reaction
DEG C, keep 30min that enzyme-deactivating is obtained enzymolysis liquid, wherein the first enzyme preparation weight is the 0.15% of giant salamander bone and flesh weight, institute
0.15% that second enzyme weight of formulation is giant salamander bone and flesh weight is stated, the third enzyme preparation weight is giant salamander bone and flesh weight
0.04%；After the first enzyme preparation is added into enzymatic vessel and stirs 15min, adds the second enzyme preparation and carry out shearing 3h, add
Third enzyme preparation carries out shearing 2h；It is preferably in the present invention, first enzyme preparation is neutral proteinase, the second enzyme
Preparation is alkali protease, and the third enzyme preparation is de- bitter protease；
Filtering: gained enzymolysis liquid in above-mentioned steps S5 is cooled to 50 DEG C, deodorization processing is carried out, by plate-frame filtering by step S6
Machine is precoated with diatomite, and 0.5h is mixed in 2% active carbon that giant salamander weight is added in enzymolysis liquid, and repressurization is passed through
Flame filter press, until filtrate is in yellow clear liquid；
Step S7, filtering: by yellow clear liquid obtained in above-mentioned steps S6, using the permeable membranes such as composite tube type film into
The solute and solvent of row certain molecular weight separate, and are 1.6Bar into film pressure, and film pressure is 0.9Bar out, and temperature is 57 DEG C, dense
Flow quantity is 350LPM, and clear liquid flow is 70LPM；
Filtering: solvent after separating in above-mentioned steps S7 is used nanofiltration membrane to carry out 150Dal small organic molecule by step S8 again
Separation is 10Bar into film pressure, and film pressure is 10Bar out, and starting dope flow at first 5 minutes is 20LPM, clear liquid flow >
100LPM, preferably 101LPM, filter pressure 0.35MPA；
Step S9 is concentrated under reduced pressure: dense to filtrate by the filtrate isolated in above-mentioned steps S8 using dual-effect concentrator decompression heating
Centrifugal spray drying is carried out when being reduced to soluble solid content up to 40%, the centrifugal spray drying control inlet temperature is
160 DEG C, air outlet temperature is 90 DEG C and feed rate is 100r/min, concentration the spray-dried obtained albumen powder of filtrate be
42.05g, at rate 42.05%.
Embodiment 2
The present invention provides a kind of method of abstraction function albumen from giant salamander, and the method follows the steps below:
Step S1, pre-treatment: removal giant salamander internal organ and four limbs, blood put it is net after, carry out stripping and slicing, then Giant salamander bone meat piece head is put into
In skeleton breaking machine, Giant salamander bone meat gruel is obtained, 1kg is weighed；
Step S2, soda acid processing: putting into deliming tank for the Giant salamander bone meat gruel in above-mentioned steps S1, after pure water cleaning is added, then
The processing of 2kg food grade hydrochloric acid (concentration is 10% or so after dilution) is added, time 0.5h is disposed and pure water cleaning two is added
Secondary, it is 10.0 that the food grade sodium hydroxide for adding 1% mass fraction, which is neutralized, adjusts pH value, is stirred while adding, often
10min measurement is primary, until pH value is identical twice, pH value washes with water after stablizing 0.5h, until pH value is washed till 7.5；
Step S3, hot water extract: the material in above-mentioned steps S2 being gone to extractor from deliming tank, giant salamander bone and flesh weight is added
3.5 times of pure water are warming up to 95 DEG C, persistently extract 3h, persistently stirred with agitating paddle in the process；
Liquid phase separation: step S4 after the completion of above-mentioned steps S3 extraction, stops heating, stops stirring, tank to be extracted is down to room
Temperature stands 12h and waits being layered, upper layer grease is centrifugated, and oil reservoir centrifugation is excluded to obtain extracting solution using centrifuge；
Step S5, enzymatic hydrolysis: putting into enzymatic vessel for the extracting solution in above-mentioned steps S4, be warming up to 60 DEG C, is adjusted to the best anti-of enzyme
Condition is answered, according still further to the first enzyme preparation, the second enzyme preparation, third enzyme preparation is sequentially sequentially added, is warming up to 95 after reaction
DEG C, keep 30min that enzyme-deactivating is obtained enzymolysis liquid, wherein the 0.2%(that the first enzyme preparation weight is giant salamander bone and flesh weight is
2g), the second enzyme weight of formulation is 0.2%(, that is, 2g of giant salamander bone and flesh weight), the third enzyme preparation weight is giant salamander bone and flesh
0.05%(, that is, 0.5g of weight)；After the first enzyme preparation is added into enzymatic vessel and stirs 15min, add the second enzyme preparation into
Row shearing 3h, adds third enzyme preparation and carries out shearing 2h；It is preferably in the present invention, first enzyme preparation is neutral egg
White enzyme, second enzyme preparation are alkali protease, and the third enzyme preparation is de- bitter protease；
Filtering: gained enzymolysis liquid in above-mentioned steps S5 is cooled to 55 DEG C, deodorization processing is carried out, by plate-frame filtering by step S6
Machine is precoated with diatomite, and 0.5h is mixed in 3% active carbon that giant salamander weight is added in enzymolysis liquid, and repressurization is passed through
Flame filter press, until filtrate is in yellow clear liquid；
Step S7, filtering: by yellow clear liquid obtained in above-mentioned steps S6, using the permeable membranes such as composite tube type film into
The solute and solvent of row certain molecular weight separate, and are 1.6Bar into film pressure, and film pressure is 0.8Bar out, and temperature is 55 DEG C, dense
Flow quantity is 300LPM, and clear liquid flow is 79LPM；
Filtering: solvent after separating in above-mentioned steps S7 is used nanofiltration membrane to carry out 600Dal small organic molecule by step S8 again
Separation is 12Bar into film pressure, and film pressure is 10Bar out, and starting dope flow at first 5 minutes is 23LPM, clear liquid flow >
100LPM, preferably 101LPM, filter pressure 0.35MPA；
Step S9 is concentrated under reduced pressure: dense to filtrate by the filtrate isolated in above-mentioned steps S8 using dual-effect concentrator decompression heating
Centrifugal spray drying is carried out when being reduced to soluble solid content up to 50%, the centrifugal spray drying control inlet temperature is
175 DEG C, air outlet temperature is 95 DEG C and feed rate is 200r/min；The spray-dried obtained albumen powder of filtrate, which is concentrated, is
530.6g, at rate 53.06%.
Embodiment 3
The present invention provides a kind of method of abstraction function albumen from giant salamander, and the method follows the steps below:
Step S1, pre-treatment: removal giant salamander internal organ and four limbs, blood put it is net after, carry out stripping and slicing, then Giant salamander bone meat piece head is put into
In skeleton breaking machine, Giant salamander bone meat gruel is obtained, 1kg is weighed；
Step S2, soda acid processing: putting into deliming tank for the Giant salamander bone meat gruel in above-mentioned steps S1, after pure water cleaning is added, then
The processing of 2kg food grade hydrochloric acid (concentration is 10% or so after dilution) is added, time 0.5h is disposed and pure water cleaning two is added
Secondary, it is 10.0 that the food grade sodium hydroxide for adding 1% mass fraction, which is neutralized, adjusts pH value, is stirred while adding, often
10min measurement is primary, until pH value is identical twice, pH value washes with water after stablizing 0.5h, until pH value is washed till 8.0；
Step S3, hot water extract: the material in above-mentioned steps S2 being gone to extractor from deliming tank, the 4 of giant salamander bone and flesh weight are added
Times pure water, is warming up to 95 DEG C, persistently extracts 3h, persistently stirred with agitating paddle in the process；
Liquid phase separation: step S4 after the completion of above-mentioned steps S3 extraction, stops heating, stops stirring, tank to be extracted is down to room
Temperature stands 12h and waits being layered, upper layer grease is centrifugated, and oil reservoir centrifugation is excluded to obtain extracting solution using centrifuge；
Step S5, enzymatic hydrolysis: putting into enzymatic vessel for the extracting solution in above-mentioned steps S4, be warming up to 65 DEG C, is adjusted to the best anti-of enzyme
Condition is answered, according still further to the first enzyme preparation, the second enzyme preparation, third enzyme preparation is sequentially sequentially added, is warming up to 95 after reaction
DEG C, keep 30min that enzyme-deactivating is obtained enzymolysis liquid, wherein the 0.25%(that the first enzyme preparation weight is giant salamander bone and flesh weight is
2.5g), the second enzyme weight of formulation is 0.25%(, that is, 2.5g of giant salamander bone and flesh weight), the third enzyme preparation weight is big
0.06%(, that is, 0.6g of salamander bone and flesh weight)；After the first enzyme preparation is added into enzymatic vessel and stirs 15min, second enzyme is added
Preparation carries out shearing 3h, adds third enzyme preparation and carries out shearing 2h；It is preferably in the present invention, first enzyme preparation is
Neutral proteinase, second enzyme preparation are alkali protease, and the third enzyme preparation is de- bitter protease；
Filtering: gained enzymolysis liquid in above-mentioned steps S5 is cooled to 60 DEG C, deodorization processing is carried out, by plate-frame filtering by step S6
Machine is precoated with diatomite, and 0.5h is mixed in 4% active carbon that giant salamander weight is added in enzymolysis liquid, and repressurization is passed through
Flame filter press, until filtrate is in yellow clear liquid；
Step S7, filtering: by yellow clear liquid obtained in above-mentioned steps S6, using the permeable membranes such as composite tube type film into
The solute and solvent of row certain molecular weight separate, and are 1.7Bar into film pressure, and film pressure is 1.0Bar out, and temperature is 60 DEG C, dense
Flow quantity is 400LPM, and clear liquid flow is 90LPM；
Filtering: solvent after separating in above-mentioned steps S7 is used nanofiltration membrane to carry out 1000Dal small organic molecule by step S8 again
Separation is 20Bar into film pressure, and film pressure is 20Bar out, and starting dope flow at first 5 minutes is 40LPM, clear liquid flow >
100LPM, preferably 101LPM, filter pressure 0.35MPA；
Step S9 is concentrated under reduced pressure: dense to filtrate by the filtrate isolated in above-mentioned steps S8 using dual-effect concentrator decompression heating
Centrifugal spray drying is carried out when being reduced to soluble solid content up to 60%, the centrifugal spray drying control inlet temperature is
190 DEG C, air outlet temperature is 100 DEG C and feed rate is 300r/min；The spray-dried obtained albumen powder of filtrate, which is concentrated, is
490.5g, at rate 49.05%
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, it is possible to understand that
These embodiments can be carried out with a variety of variations, modification, replacement without departing from the principles and spirit of the present invention and become
Type, protection scope of the present invention are defined by the appended claims and the equivalents thereof.
Claims (9)
1. a kind of method of the abstraction function albumen from giant salamander, which is characterized in that the method follows the steps below:
After step S1, removal giant salamander internal organ and four limbs, blood are put only, stripping and slicing is carried out, then Giant salamander bone meat piece head is put into skeleton breaking machine
In, obtain Giant salamander bone meat gruel；
Giant salamander bone meat gruel in above-mentioned steps S1 is put into deliming tank by step S2, after pure water cleaning is added, adds giant salamander weight
Hydrochloric acid carries out deliming processing after 2 times of dilution of amount, and time 0.5h is disposed and pure water cleaning is added twice, then with 1% mass
It is 10.0 that the food grade sodium hydroxide of score, which is neutralized, adjusts pH value, is washed with water after alkali process 0.5h, until pH value is washed till
7.0~8.0；
Material in above-mentioned steps S2 is gone to extractor from deliming tank by step S3, and 3~4 times that giant salamander bone and flesh weight is added are pure
Water is warming up to 95 DEG C, persistently extracts 3h, persistently stirred with agitating paddle in the process；
Step S4 closes agitating paddle after the completion of above-mentioned steps S3 extraction, stops stirring, and tank to be extracted is down to room temperature, stands
12h waits being layered, and upper layer grease is centrifugated, extracting solution is obtained；
Extracting solution in above-mentioned steps S4 is put into enzymatic vessel, is warming up to 55~65 DEG C, is adjusted to the optimum response of enzyme by step S5
Condition is warming up to 95 DEG C according still further to the first enzyme preparation, the second enzyme preparation, third enzyme preparation is sequentially sequentially added after reaction,
Keep 30min that enzyme-deactivating is obtained enzymolysis liquid；
Gained enzymolysis liquid in above-mentioned steps S5 is cooled to 50~60 DEG C, deodorization processing is carried out, by flame filter press by step S6
It is precoated with diatomite, 0.5h is mixed in 2~4% active carbon that giant salamander weight is added in enzymolysis liquid, and repressurization is passed through
Flame filter press, until filtrate is in yellow clear liquid；
Yellow clear liquid obtained in above-mentioned steps S6 is carried out one using permeable membranes such as composite tube type films by step S7
Determine solute and the solvent separation of molecular weight；
Solvent after separating in above-mentioned steps S7 is used nanofiltration membrane to carry out 150~1000Dal small organic molecule by step S8 again
Separation；
Step S9 is concentrated to solvable by the filtrate isolated in above-mentioned steps S8 using dual-effect concentrator decompression heating to filtrate
Property solid content up to 40~60% when carry out centrifugal spray drying, albumen powder is made.
2. a kind of method of abstraction function albumen from giant salamander according to claim 1, which is characterized in that the step S5
In the first enzyme preparation and the second enzyme preparation be protein incision enzyme, third enzyme preparation be albumen excision enzyme.
3. -2 a kind of described in any item methods of the abstraction function albumen from giant salamander according to claim 1, which is characterized in that institute
0.15~0.25% that the first enzyme preparation weight is giant salamander bone and flesh weight is stated, the second enzyme weight of formulation is giant salamander bone and flesh weight
0.15~0.25%, the third enzyme preparation weight be giant salamander bone and flesh weight 0.04~0.06%.
4. -2 a kind of described in any item methods of the abstraction function albumen from giant salamander according to claim 1, which is characterized in that
After the first enzyme preparation is added in enzymatic vessel and stirs 15min, adds the second enzyme preparation and carry out shearing 3h, add third enzyme system
Agent carries out shearing 2h.
5. -2 a kind of described in any item methods of the abstraction function albumen from giant salamander according to claim 1, which is characterized in that institute
Stating the first enzyme preparation is neutral proteinase, and second enzyme preparation is alkali protease, and the third enzyme preparation is de- bitter albumen
Enzyme.
6. a kind of method of abstraction function albumen from giant salamander according to claim 1, which is characterized in that the step S9
Described in centrifugal spray drying control inlet temperature be 160~190 DEG C, air outlet temperature be 90~100 DEG C and charging speed
Rate is 100~300r/min.
7. a kind of method of abstraction function albumen from giant salamander according to claim 1, which is characterized in that the step S7
In into film pressure be 1.6~1.7/Bar, out film pressure be 0.8~1.0 Bar, dope flow be 300~400LPM, clear liquid stream
Amount is 70~90LPM.
8. a kind of method of abstraction function albumen from giant salamander according to claim 1, which is characterized in that the step S8
In into film pressure be 10~20Bar, film pressure is 10~20Bar out, and starting at first 5 minutes dope flow is 20~40LPM, clearly
Flow quantity > 100LPM, filter pressure 0.35MPA.
9. a kind of method of abstraction function albumen from giant salamander according to claim 1, which is characterized in that the step S2
Hydrochloric acid after middle dilution, concentrated hydrochloric acid: water=1:3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910313221.7A CN109943616A (en) | 2019-04-18 | 2019-04-18 | A method of the abstraction function albumen from giant salamander |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910313221.7A CN109943616A (en) | 2019-04-18 | 2019-04-18 | A method of the abstraction function albumen from giant salamander |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109943616A true CN109943616A (en) | 2019-06-28 |
Family
ID=67014431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910313221.7A Withdrawn CN109943616A (en) | 2019-04-18 | 2019-04-18 | A method of the abstraction function albumen from giant salamander |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109943616A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111802505A (en) * | 2020-07-28 | 2020-10-23 | 威海市宇王集团海洋生物工程有限公司 | Industrialized abalone peptide extraction method |
| CN111808185A (en) * | 2020-07-08 | 2020-10-23 | 威海市宇王集团海洋生物工程有限公司 | Method for extracting elastin peptide from bovine cartilage |
| CN112176017A (en) * | 2020-10-27 | 2021-01-05 | 威海市宇王集团海洋生物工程有限公司 | Method for efficiently extracting protein peptide from sea cucumber intestines |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342904A (en) * | 2011-10-09 | 2012-02-08 | 湖南今珠生物科技有限公司 | Preparation method for soluble pearl powder |
| CN102643890A (en) * | 2012-04-28 | 2012-08-22 | 韩金光 | Collagen polypeptide and preparation method thereof |
| CN104206641A (en) * | 2014-08-25 | 2014-12-17 | 张家界金鲵生物工程股份有限公司 | Megalobatrachus protein polypeptide powder and preparation method thereof |
| CN108611390A (en) * | 2018-04-24 | 2018-10-02 | 浙江大学 | A method of preparing low bitter taste buffalo's milk casein antioxidant peptide powder |
| CN109097426A (en) * | 2018-08-31 | 2018-12-28 | 宁波海之源生物科技有限公司 | A kind of preparation method of giant salamander protein peptides |
-
2019
- 2019-04-18 CN CN201910313221.7A patent/CN109943616A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342904A (en) * | 2011-10-09 | 2012-02-08 | 湖南今珠生物科技有限公司 | Preparation method for soluble pearl powder |
| CN102643890A (en) * | 2012-04-28 | 2012-08-22 | 韩金光 | Collagen polypeptide and preparation method thereof |
| CN104206641A (en) * | 2014-08-25 | 2014-12-17 | 张家界金鲵生物工程股份有限公司 | Megalobatrachus protein polypeptide powder and preparation method thereof |
| CN108611390A (en) * | 2018-04-24 | 2018-10-02 | 浙江大学 | A method of preparing low bitter taste buffalo's milk casein antioxidant peptide powder |
| CN109097426A (en) * | 2018-08-31 | 2018-12-28 | 宁波海之源生物科技有限公司 | A kind of preparation method of giant salamander protein peptides |
Non-Patent Citations (1)
| Title |
|---|
| 李龙等: "海洋生物源胶原的医学应用研究进展", 《中国海洋药物》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808185A (en) * | 2020-07-08 | 2020-10-23 | 威海市宇王集团海洋生物工程有限公司 | Method for extracting elastin peptide from bovine cartilage |
| CN111802505A (en) * | 2020-07-28 | 2020-10-23 | 威海市宇王集团海洋生物工程有限公司 | Industrialized abalone peptide extraction method |
| CN112176017A (en) * | 2020-10-27 | 2021-01-05 | 威海市宇王集团海洋生物工程有限公司 | Method for efficiently extracting protein peptide from sea cucumber intestines |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107549812B (en) | A skin caring and antiaging food prepared from acerola cherry extract, Haematococcus pluvialis and fish collagen peptide, and its preparation method | |
| CN109943616A (en) | A method of the abstraction function albumen from giant salamander | |
| KR20180018055A (en) | Anti-wrinkle Cosmetic Composition Comprising Functional Components from Sturgeon, Method for Obtaining the Functional Components and Method for Producing Anti-wrinkle Cosmetic Using the Functional Components | |
| CN106282287B (en) | Method for extracting bioactive polypeptide of ginkgo | |
| CN105018555A (en) | Preparation method of giant salamander skin collagen peptide | |
| CN105341314A (en) | Buckwheat bran active protein extraction and separation technology | |
| CN104706567B (en) | The method and product of pearl foetal sac liquid are extracted in a kind of pearl foetal sac from mother pearl shellfish | |
| CN103493969A (en) | Method for producing giant salamander polypeptide powder | |
| CN107446976A (en) | The preparation method of giant salamander polypeptide powder | |
| KR20230136092A (en) | Method for extracting polydeoxyribonucleotides and polynucleotides derived from algae with the effect of neovascularization and cell regeneration | |
| CN109022524A (en) | A kind of extraction process of hornpout collagen peptide | |
| TW201703640A (en) | Method for manufacturing fish scale processed goods | |
| CN106387304B (en) | A kind of method of high-pressure pulse electric coupling biological enzymolysis preparation abalone internal organ phosphatide | |
| CN111419760A (en) | Fresh black tiger leaf extracting solution and preparation method and application thereof | |
| CN105411896B (en) | A kind of complex emulsions rich in eucommia Bark male flower skin care activity ingredient and preparation method thereof | |
| JP5131676B2 (en) | Method for producing a polysaccharide-containing material containing chitin / chitosan using Geotricham | |
| CN108354857A (en) | Water beautifying liquid and preparation method thereof is refined after a kind of extraction of witloof polysaccharide | |
| CN109880871A (en) | A method of small-molecular peptides are extracted by raw material of euphausia superba powder | |
| CN110387397B (en) | Preparation method of sheep skin collagen oligopeptide | |
| CN110669813A (en) | Yak rib small molecule peptide and extraction method thereof | |
| CN108888554B (en) | Stem cell beauty emulsion with skin tendering and spot lightening functions and preparation method thereof | |
| CN101427819A (en) | Holothurian essence and method of producing the same | |
| CN100376170C (en) | Edible watural silk protein powder and preparation method of natural silk extract | |
| KR102584027B1 (en) | Method for extracting polydeoxyribonucleotides and polynucleotides derived from algae with the effect of neovascularization and cell regeneration | |
| CN113122604B (en) | Sea intestine glue and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190628 |
|
| WW01 | Invention patent application withdrawn after publication |