CN109022524A - A kind of extraction process of hornpout collagen peptide - Google Patents
A kind of extraction process of hornpout collagen peptide Download PDFInfo
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- CN109022524A CN109022524A CN201810884175.1A CN201810884175A CN109022524A CN 109022524 A CN109022524 A CN 109022524A CN 201810884175 A CN201810884175 A CN 201810884175A CN 109022524 A CN109022524 A CN 109022524A
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- Prior art keywords
- fish
- skin
- hornpout
- collagen
- collagen peptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The present invention discloses a kind of extraction process of hornpout collagen peptide, this method is mainly the fish-skin waste after utilizing hornpout to process, first except non-collagen, decoloration, the degreasing etc. in peeling fish skin are handled, then progress enzyme hydrolysis, enzyme-deactivating, adjusting isoelectric point, centrifugal concentrating, cryogenic vacuum freeze-drying, low-temperature airflow crush acquisition finished product.Fish-skin after the present invention processes catfish based article carries out high-valued intensive processing utilization, extract the II type purity raw produce with bioactivity that is up to 93.5% or more, the high-valued intensive processing significant effect of fish-skin waste: the wide model of product is for the industries such as medicine, health care food, cosmetics and food additives；This item extraction process heavy metal free is exceeded with medicament residue, reaches the safe and healthy standard of state food, and processing waste discharge meets national I to II class discharge standard；Equipment needed for this extraction process process is simple, low production cost.
Description
Technical field
The present invention relates to biological enzymolysis extractive technique field more particularly to a kind of extractions of hornpout collagen peptide
Technique.
Background technique
Collagen is a kind of biological polymer substance, and having a variety of biological functions is to play the part of knot in animal connective tissue
It is combined the role knitted.Collagen is that one of most critical raw material of biotech industry and demand are very huge
Best medical, moisturizing, reparation, anti-aging, the materials such as nutrition.Be maintain skin and histoorgan form, structure it is main
Ingredient, and repair the important source material substance of each injury tissue.
Past obtains natural collagen protein and its main path of collagen peptide derives from livestock and poultry animal skin tissue, but due to phase
Domestic animals disease and certain religious beliefs are closed, limits people to land there is also the possibility of infection rabid ox disease and other related diseases
The use of raw mammalian collagen and its product.The method for gradually turning to the collagen peptides such as fish skin, squama now,
Isin glue collagen is substantially better than the collagen of terrestrial animal in some respects, for example has the spies such as low antigenicity, hypoallergenic
Property, it may gradually substitute terrestrial animal collagen.
Whole world Silurformes fish belong to no squama economic fish up to more than 100 kinds, hornpout have the inclined meat of omnivorousness,
Growth is fast, wide adaptation range, meat nutrition are abundant, moves in water body that its protective film is fish-skin without squama, and fish-skin contains abundant
Collagen.Silurformes fish are easy to process at various aquatic products without piercing between flesh in the world, but after fish-skin is in processing
Waste or the processing of low side aquatic product, easily cause the wasting of resources and environmental pollution, have only a few processing enterprise using acid at present
The organic solvents such as alkali extract the collagen of hornpout fish-skin, relatively easily cause environmental pollution, product quality purity is low to be contained with impurity
Amount height and chemical contamination etc. generate side effect to human body.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of hornpout fish skin collagen proposed
The extraction process of protein peptides.
To achieve the goals above, present invention employs following technical solutions:
A kind of extraction process of hornpout collagen peptide, by extraction process process the following steps are included:
S1, by hornpout fish-skin concentration be 0.02~0.03% dietary alkali (Na2CO3), 2.3~2.7% salt
(NaCl), the mixed solution of edible hydrogen peroxide 0.07~0.09% take off under conditions of being stirred continuously for 10~12 DEG C non-
Collagen, fat, melanin are handled 4~5 hours；
S2, the fish-skin after handling well is taken off by three by above-mentioned, rinsed with clear water to neutral or acid, the fish-skin that will be handled well
Surface moisture carries out mechanical rubbing processing after being filtered dry；
S3, the hornpout fish-skin of rubbing is adjusted to pH value to 4~5, is added in the ratio of the fish-skin weight 1.5 ‰ of hornpout
The papain of 2000000 units/grams keeps the temperature enzyme hydrolysis about 24 hours under 12 DEG C of cryogenic conditions；
S4, the molecular sieve filtration that above-mentioned enzymolysis liquid is carried out to 3000D, filtering collagen peptidase hydrolyzed liquor body pass through adjusting
The enzyme isoelectric point that loses activity carries out inactivation treatment, adjusts pH value to 5.8, mechanical 4500 revs/min of centrifugation Isin glue collagens
Peptide colloidal；
S5, -45 DEG C of cryogenic vacuum freeze-dryings, air-flows ultramicro grinding institute are carried out to the collagen peptide of colloidal after centrifugation
Obtain collagen peptide powdery finished product.
Fish-skin surface moisture processing method is to be filtered dry under cryogenic conditions or shaker vibration sieves solid carbon dioxide point in the step S2.
It is that mechanical meat grinder rubs that fish-skin, which rubs method, in the step S2.
Compared with prior art, the beneficial effects of the present invention are:
1. the fish-skin after the present invention is processed using hornpout extracts collagen peptide as raw material, keep the fish-skin of hornpout sufficiently sharp
With the high-valued product of generation；
2. the method using enzyme hydrolysis extracts active principle, overcome toxic for the second time in traditional chemical routes extraction process
The technical problem of hazardous drugs and heavy-metal residual, product safety have no toxic side effect；
3. application enzyme hydrolysis of the present invention extracts the Wastewater on Ecological Environmental discharged after collagen and does not generate the dirt of face source
Dye reaches national I to II grade of discharge standard；
4. the present invention eats hydrogen peroxide solution removal non-collagen, fat, decoloration using alkali combination salt is released, improve
Extract the purity of collagen peptide, low fat, without melanin, typeⅡ Collagen peptide purity is up to 95% or more, average molecular
Amount range is 500~3000D (dalton)；
5. the biology that the entire extraction process of collagen peptide of the present invention carries out maintaining under cryogenic collagen peptide is living
Property；
6. the collagen peptide raw produce scope of use that the present invention obtains is wide, can be widely used for medicine, food additives,
The industries such as cosmetics, health care product；
7. extract equipment needed for the present invention is simpler, easy to operate, raw material sources range is wide, laborsaving low with production cost.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Embodiment 1: the embodiment of the present invention takes 5 kilograms of hornpout fish skin raw material, is 0.025% dietary alkali with concentration
(Na2CO3), 2.5% salt (NaCl), the mixed solution of edible hydrogen peroxide 0.09% and raw material press 10:1, not at 10~12 DEG C
De- non-collagen, fat, melanin is handled under conditions of disconnected stirring to handle 4~5 hours.Three de- treated fish-skin clear water
Repeatedly washing rubs fish-skin with machinery until the pH value 5.0 of fish-skin, has moisture with being filtered dry on fish-skin after pure sterile water wash
Pure sterile water is added in raw material and water 1:8.5 ratio, then adds 2,000,000 units/grams in 1.5 ‰ ratios of raw material substrate weight
7.5 grams of papain, enzyme hydrolysis 24 hours under 12 DEG C of cryogenic conditions, enzymolysis liquid through overregulate enzyme inactivation isoelectric point go out
It is living, then collagen optimal pH 5.8 is adjusted, it is centrifuged 12 minutes with 4500 revs/min of centrifuge, the collagen of slurry shape
Peptide carries out -40 DEG C of low vacuum freeze-dryings, air-flows pulverize acquisition finished product, finally obtains the recovery rate of hornpout fish skin raw material
38.12%, 1906 grams of the collagen peptide with bioactivity.TypeⅡ Collagen peptide molecular weight is 500~3000 dalton.
Embodiment 2: the embodiment of the present invention takes 5 kilograms of hornpout fish skin raw material, is 0.02% dietary alkali with concentration
(Na2CO3), 2.3% salt (NaCl), the mixed solution of edible hydrogen peroxide 0.07% and raw material press 11:1, not at 10~12 DEG C
De- non-collagen, fat, melanin is handled under conditions of disconnected stirring to handle 4~5 hours.Three de- treated fish-skin clear water
Repeatedly washing rubs fish-skin with machinery until the pH value 4.0 of fish-skin, has moisture with being filtered dry on fish-skin after pure sterile water wash
Pure sterile water is added in raw material and water 1:10 ratio, then adds 2,000,000 units/grams in 1.0 ‰ ratios of raw material substrate weight
5 grams of papain, enzyme hydrolysis 24 hours under 12 DEG C of cryogenic conditions, enzymolysis liquid through overregulate enzyme inactivation isoelectric point go out
It is living, then collagen optimal pH 5.8 is adjusted, it is centrifuged 12 minutes with 4500 revs/min of centrifuge, the collagen of slurry shape
Peptide carries out -40 DEG C of low vacuum freeze-dryings, air-flows pulverize acquisition finished product, finally obtains the recovery rate of hornpout fish skin raw material
36.19%, 1809.5 grams of the collagen peptide with bioactivity.TypeⅡ Collagen peptide molecular weight is 500~3000 dalton.
Embodiment 3: the embodiment of the present invention takes 5 kilograms of hornpout fish skin raw material, is 0.03% dietary alkali with concentration
(Na2CO3), 2.6% salt (NaCl), the mixed solution of edible hydrogen peroxide 0.09% and raw material press 13:1, not at 10~12 DEG C
De- non-collagen, fat, melanin is handled under conditions of disconnected stirring to handle 4~5 hours.Three de- treated fish-skin clear water
Repeatedly washing rubs fish-skin with machinery until the pH value 4.5 of fish-skin, has moisture with being filtered dry on fish-skin after pure sterile water wash
Pure sterile water is added in raw material and water 1:10 ratio, then adds 2,000,000 units/grams in 1.2 ‰ ratios of raw material substrate weight
6 grams of papain, enzyme hydrolysis 24 hours under 12 DEG C of cryogenic conditions, enzymolysis liquid through overregulate enzyme inactivation isoelectric point go out
It is living, then adjust collagen optimal pH, be centrifuged 12 minutes with 4500 revs/min of centrifuge, the collagen peptide of slurry shape into
Row -40 DEG C of low vacuum freeze-dryings, air-flows, which pulverize, obtains finished product, finally obtains the recovery rate of hornpout fish skin raw material
37.05%, 1852.5 grams of the collagen peptide with bioactivity.TypeⅡ Collagen peptide molecular weight is 500~3000 dalton.
Embodiment 4: the embodiment of the present invention takes 5 kilograms of hornpout fish skin raw material, is 0.025% dietary alkali with concentration
(Na2CO3), 2.5% salt (NaCl), the mixed solution of edible hydrogen peroxide 0.085% and raw material press 15:1, not at 10~12 DEG C
De- non-collagen, fat, melanin is handled under conditions of disconnected stirring to handle 4~5 hours.Three de- treated fish-skin clear water
Repeatedly washing rubs fish-skin with machinery until the pH value 4.0 of fish-skin, has moisture with being filtered dry on fish-skin after pure sterile water wash
Pure sterile water is added in raw material and water 1:10 ratio, then adds 2,000,000 units/grams in 2.0 ‰ ratios of raw material substrate weight
10 grams of papain, enzyme hydrolysis 24 hours under 12 DEG C of cryogenic conditions, enzymolysis liquid through overregulate enzyme inactivation isoelectric point go out
It is living, then adjust collagen optimal pH, be centrifuged 12 minutes with 4500 revs/min of centrifuge, the collagen peptide of slurry shape into
Row -40 DEG C of low vacuum freeze-dryings, air-flows, which pulverize, obtains finished product, finally obtains the recovery rate of hornpout fish skin raw material
37.92%, 1896 grams of the collagen peptide with bioactivity.TypeⅡ Collagen peptide molecular weight is 500~3000 dalton.
In above each embodiment, when washing with water three de- hornpout fish-skins, slurry is rubbed after being then filtered dry moisture,
The equipment knot hornpout fish-skin of selection tool physics crushing function is crushed, and does not limit distinctive equipment, such as meat grinder, tissue
Bruisher, strand body mill etc..
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (3)
1. a kind of extraction process of hornpout collagen peptide, which is characterized in that by extraction process process include following step
It is rapid:
S1, by hornpout fish-skin concentration be 0.02~0.03% dietary alkali (Na2CO3), 2.3~2.7% salt (NaCl), food
The mixed solution of grade hydrogen peroxide 0.07~0.09%, carried out under conditions of being stirred continuously for 10~12 DEG C de- non-collagen,
Fat, melanin are handled 4~5 hours；
S2, the fish-skin after handling well is taken off by three by above-mentioned, rinsed with clear water to neutral or acid, the fish-skin surface that will be handled well
Moisture carries out mechanical rubbing processing after being filtered dry；
S3, the hornpout fish-skin of rubbing is adjusted to pH value to 4~5, in the ratio addition 200 of the fish-skin weight 1.5 ‰ of hornpout
The papain of ten thousand units/grams keeps the temperature enzyme hydrolysis about 24 hours under 12 DEG C of cryogenic conditions；
S4, the molecular sieve filtration that above-mentioned enzymolysis liquid is carried out to 3000D, filtering collagen peptidase hydrolyzed liquor body are lost by adjusting enzyme
Deactivation isoelectric point carries out inactivation treatment, adjusting pH value to 5.8, mechanical 4500 revs/min of centrifugation Isin glue collagen peptide glue
Body shape；
S5, the collagen peptide of colloidal after centrifugation is carried out acquired in -45 DEG C of cryogenic vacuum freeze-dryings, air-flows ultramicro grinding
Collagen peptide powdery finished product.
2. a kind of extraction process of hornpout collagen peptide according to claim 1, it is characterised in that: the step
Fish-skin surface moisture processing method is to be filtered dry under cryogenic conditions or shaker vibration sieves solid carbon dioxide point in rapid S2.
3. a kind of extraction process of hornpout collagen peptide according to claim 1, it is characterised in that: the step
It is that mechanical meat grinder rubs that fish-skin, which rubs method, in rapid S2.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810884175.1A CN109022524A (en) | 2018-08-06 | 2018-08-06 | A kind of extraction process of hornpout collagen peptide |
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| CN201810884175.1A CN109022524A (en) | 2018-08-06 | 2018-08-06 | A kind of extraction process of hornpout collagen peptide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231585A (en) * | 2022-01-06 | 2022-03-25 | 广西金海盈高新技术有限公司 | Preparation process of fish collagen peptide of channel catfish |
| CN114304572A (en) * | 2022-01-10 | 2022-04-12 | 广西金海盈食品有限公司 | Preparation method of fish and skull bone paste soup of channel catfish |
| CN114304540A (en) * | 2022-01-10 | 2022-04-12 | 广西金海盈食品有限公司 | Preparation method of crispy channel catfish skin |
| CN115403668A (en) * | 2022-09-28 | 2022-11-29 | 湖北兴祥生物科技有限公司 | Extraction process of bioactive collagen peptide of channel catfish swim bladder |
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| CN101607993A (en) * | 2009-07-27 | 2009-12-23 | 湖北省水产科学研究所 | A kind of extraction process of collagen of channel catfish skin |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101607993A (en) * | 2009-07-27 | 2009-12-23 | 湖北省水产科学研究所 | A kind of extraction process of collagen of channel catfish skin |
Non-Patent Citations (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231585A (en) * | 2022-01-06 | 2022-03-25 | 广西金海盈高新技术有限公司 | Preparation process of fish collagen peptide of channel catfish |
| CN114304572A (en) * | 2022-01-10 | 2022-04-12 | 广西金海盈食品有限公司 | Preparation method of fish and skull bone paste soup of channel catfish |
| CN114304540A (en) * | 2022-01-10 | 2022-04-12 | 广西金海盈食品有限公司 | Preparation method of crispy channel catfish skin |
| CN115403668A (en) * | 2022-09-28 | 2022-11-29 | 湖北兴祥生物科技有限公司 | Extraction process of bioactive collagen peptide of channel catfish swim bladder |
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