CN105753971A - Collagen extraction method - Google Patents
Collagen extraction method Download PDFInfo
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- CN105753971A CN105753971A CN201610218113.8A CN201610218113A CN105753971A CN 105753971 A CN105753971 A CN 105753971A CN 201610218113 A CN201610218113 A CN 201610218113A CN 105753971 A CN105753971 A CN 105753971A
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 42
- 108010035532 Collagen Proteins 0.000 title claims abstract description 42
- 238000000605 extraction Methods 0.000 title abstract description 12
- 229920001436 collagen Polymers 0.000 title abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 55
- 241000251468 Actinopterygii Species 0.000 claims abstract description 37
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 25
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 238000007710 freezing Methods 0.000 claims abstract description 19
- 230000008014 freezing Effects 0.000 claims abstract description 19
- 238000000746 purification Methods 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 19
- 238000005119 centrifugation Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000002244 precipitate Substances 0.000 claims abstract description 10
- 230000001105 regulatory effect Effects 0.000 claims abstract description 10
- 235000013372 meat Nutrition 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 23
- 238000000502 dialysis Methods 0.000 claims description 18
- 239000011347 resin Substances 0.000 claims description 18
- 229920005989 resin Polymers 0.000 claims description 18
- 239000006228 supernatant Substances 0.000 claims description 18
- 238000000108 ultra-filtration Methods 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 10
- 239000004367 Lipase Substances 0.000 claims description 9
- 102000004882 Lipase Human genes 0.000 claims description 9
- 108090001060 Lipase Proteins 0.000 claims description 9
- 239000003957 anion exchange resin Substances 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 9
- 238000009413 insulation Methods 0.000 claims description 9
- 235000019421 lipase Nutrition 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 9
- 239000000049 pigment Substances 0.000 claims description 9
- 238000001556 precipitation Methods 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 238000000859 sublimation Methods 0.000 claims description 9
- 230000008022 sublimation Effects 0.000 claims description 9
- 238000002604 ultrasonography Methods 0.000 claims description 9
- 239000013505 freshwater Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000000227 grinding Methods 0.000 abstract 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 210000001835 viscera Anatomy 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241000252335 Acipenser Species 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 210000004712 air sac Anatomy 0.000 description 2
- 241001086826 Branta bernicla Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- 241000720946 Hypophthalmichthys molitrix Species 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 241001275898 Mylopharyngodon piceus Species 0.000 description 1
- MMOXZBCLCQITDF-UHFFFAOYSA-N N,N-diethyl-m-toluamide Chemical compound CCN(CC)C(=O)C1=CC=CC(C)=C1 MMOXZBCLCQITDF-UHFFFAOYSA-N 0.000 description 1
- 241001214258 Pampus argenteus Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a collagen extraction method. The collagen extraction method comprises the following steps: cleaning, slicing and freezing fish; putting frozen fish slices into a low-temperature drying tank, and drying; regulating the pH value of a material liquid by virtue of a 1mol/L NaOH solution, adding compound enzyme for enzymolysis, and maintaining the temperature; carrying out grinding, centrifugation and settling, and then carrying out grinding and centrifugation once to remove precipitates, so as to obtain supernate; and carrying out sterilization, filtering, and carrying out centrifugation and purification. The collagen extraction method has the advantages that extraction materials are easily available, the yield is high, the production cost is low, the purity of collagen is high, and the extraction process is simple.
Description
Technical field
The present invention relates to the extraction process of a kind of collagen protein, particularly a kind of technique extracting collagen protein on fresh-water fishes and marine fish.
Background technology
Collagen protein is a kind of biological polymer substance, English formal name used at school Collagen.Zooblast is played the part of the role of conjunctive tissue.For one of most critical raw material of biotech industry, it also it is the very huge best raw doctor's material of demand.Its application includes raw doctor material, cosmetics, food industry, research purposes etc..
Collagen protein is a kind of extracellular protein, it is to be twisted into spiral fibrous proteins by 3 peptide chains, collagen protein is the most rich in protein of people's in-vivo content, and father's Brant (J Brandt) doctor of world's collagen protein asserts: " process of human senility is exactly the process that collagen protein runs off！" collagen protein accounts for more than the 30% of whole body gross protein.
The aminoacid such as glycine that collagen protein needs rich in human body, proline, hydroxyproline.Collagen protein is most important ingredient in extracellular matrix.
And the most rich in protein of Fish intensive amount, be equivalent to the 6% of body weight, the flesh of fish, fish skin and Air Bladder pseudosciaenae seu Acipenser all contain the collagen protein enriched, but not yet have technique fish skin, Air Bladder pseudosciaenae seu Acipenser and flesh of fish kind collagen protein fully extracted at present.Currently used extracting mode is clear water high temperature steaming, acid-hydrolysis method, alkali hydrolysis method, it is easy to become macromoleGelatin, it is unfavorable for absorption of human body, reduces its value；And product yield is low, equipment corrosion is serious, and produces secondary pollution.Said method is also all only limitted to extract from the flesh of fish, and other positions of fish cannot be extracted, and causes certain wasting of resources.
Summary of the invention
The invention aims to overcome above deficiency, design one is extracted material and is easy to get, and yield is high, production cost is low, collagen protein purity is good, the extracting method of the simple collagen protein of extraction process.
The purpose of the present invention is achieved through the following technical solutions:A kind of extracting method of collagen protein, comprising the following steps: step 1, first the flesh of fish is cleaned up, freezing after section, cooling time is 18-24 hour, and cryogenic temperature is subzero 4048 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.4-7.9, material temperature adds complex enzyme zymohydrolysis when 46-54 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 2.5-3.4:100, insulation 4-8h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3-3.5h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Further improvement of the present invention is in that: the described flesh of fish is marine fish and fresh-water fishes, the described edible position that the flesh of fish is fish.
The invention have the advantages that
Extraction material is easy to get, and yield is high, production cost is low, collagen protein purity is good, and extraction process is simple.
Detailed description of the invention:
In order to deepen the understanding of the present invention, below in conjunction with embodiment, the invention will be further described, and this embodiment is only used for explaining the present invention, is not intended that limiting the scope of the present invention.
Embodiment 1
A kind of extracting method of collagen protein, comprising the following steps: step 1, first Hypophthalmichthys molitrix meat is cleaned up, freezing after section, cooling time is 18 hours, and cryogenic temperature is subzero 48 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.4, material temperature adds complex enzyme zymohydrolysis when 54 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 2.5:100, insulation 8h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Embodiment 2
A kind of extracting method of collagen protein, comprising the following steps: step 1, first Carnis Mylopharyngodon piceus is cleaned up, freezing after section, cooling time is 19.5 hours, and cryogenic temperature is subzero 46 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.5, material temperature adds complex enzyme zymohydrolysis when 52 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 2.7:100, insulation 7.5h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Embodiment 3
A kind of extracting method of collagen protein, comprising the following steps: step 1, first Muraenesocis cinereus meat is cleaned up, freezing after section, cooling time is 20 hours, and cryogenic temperature is subzero 44 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.8, material temperature adds complex enzyme zymohydrolysis when 50 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 3.0:100, insulation 7h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3.5h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Embodiment 4
A kind of extracting method of collagen protein, comprising the following steps: step 1, first the lithosporic flesh of fish is cleaned up, freezing after section, cooling time is 22 hours, and cryogenic temperature is subzero 42 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.6, material temperature adds complex enzyme zymohydrolysis when 47 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 3.0:100, insulation 5h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3.25h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Embodiment 5
A kind of extracting method of collagen protein, comprising the following steps: step 1, first the Ankang flesh of fish is cleaned up, freezing after section, cooling time is 24 hours, and cryogenic temperature is subzero 43 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.9, material temperature adds complex enzyme zymohydrolysis when 46.5 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 3.1:100, insulation 6.5h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3.5h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Embodiment 6
A kind of extracting method of collagen protein, comprising the following steps: step 1, first Stromateoides argenteus meat is cleaned up, freezing after section, cooling time is 20 hours, and cryogenic temperature is subzero 48 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.4, material temperature adds complex enzyme zymohydrolysis when 47.5 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 3.2:100, insulation 7h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
Embodiment 7
A kind of extracting method of collagen protein, comprising the following steps: step 1, first the internal organs of fish and fish scale are cleaned up, freezing after chopping, cooling time is 20 hours, and cryogenic temperature is subzero 48 DEG C；
Step 2, the internal organs after freezing and fish scale are put in cold drying case dry, internal organs are become gaseous state with the moisture on fish scale from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.6, material temperature adds complex enzyme zymohydrolysis when 54 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 3.3:100, insulation 5h；Then with ultrasound wave, internal organs and fish scale are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3.2h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
The present invention has extraction material and is easy to get, and yield improves 3.5-5% than traditional extracting mode, and production cost is low, collagen protein purity is good, and extraction process is simple.
The another statement of applicant, the present invention illustrates the method that realizes and the apparatus structure of the present invention by above-described embodiment, but the invention is not limited in above-mentioned embodiment, does not namely mean that the present invention has to rely on said method and structure could be implemented.Person of ordinary skill in the field, it will be clearly understood that any improvement in the present invention, realizes method equivalence replacement and the interpolation of step, concrete way choice etc. selected by the present invention, all falls within protection scope of the present invention and scope of disclosure.
The present invention is not limited to above-mentioned embodiment, all employings and analog structure of the present invention and method realizes all modes of the object of the invention, all within protection scope of the present invention.
Claims (2)
1. the extracting method of a collagen protein, it is characterised in that: comprising the following steps: step 1, first cleaned up by the flesh of fish, freezing after section, cooling time is 18-24 hour, and cryogenic temperature is subzero 40-48 DEG C；
Step 2, the fish meat sheet after freezing is put in cold drying case dry, the moisture on sliced meat is become gaseous state from solid state sublimation, to remove moisture；
Step 3, regulating material liquid pH value by the NaOH solution of 1mol/L to 7.4-7.9, material temperature adds complex enzyme zymohydrolysis when 46-54 DEG C, and the mass ratio of raw material and lipase mixed enzyme is: 2.5-3.4:100, insulation 4-8h；Then with ultrasound wave, sliced meat are pulverized, pulverize liquid refrigerated centrifuger and be centrifuged, carry out again after precipitation once pulverizing and centrifugal, remove precipitate, obtain supernatant；
Step 4, the supernatant obtained in above-mentioned steps add anion exchange resin, and 3-3.5h is soaked in stirring, allows resin adsorb pigment and the fishy smell of feed liquid completely；With chromatographic column, feed liquid is separated with resin；
Step 5, sterilization, filtered after sterilization, feed liquid filtered by ultrafiltration membrane separating device, obtain ultrafiltration dialysis liquid；
Step 6, centrifugal purification: by the solution high speed frozen centrifugation after dialysis, gained is precipitated as the collagen protein of final purification.
2. the extracting method of collagen protein according to claim 1, it is characterised in that: the described flesh of fish is marine fish and fresh-water fishes, the described edible position that the flesh of fish is fish.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106509332A (en) * | 2016-12-21 | 2017-03-22 | 江苏大学 | Preparation method for silver carp meat protein soft gel |
| CN106509332B (en) * | 2016-12-21 | 2019-12-03 | 江苏大学 | A kind of preparation method of the soft gel of sillver carp proteins |
| CN109486885A (en) * | 2017-09-09 | 2019-03-19 | 上海新肌生物科技有限公司 | A kind of absorptivity reaches 96% nanoscale collagen method of purification |
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