CN106509332A - Preparation method for silver carp meat protein soft gel - Google Patents

Preparation method for silver carp meat protein soft gel Download PDF

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Publication number
CN106509332A
CN106509332A CN201611190863.5A CN201611190863A CN106509332A CN 106509332 A CN106509332 A CN 106509332A CN 201611190863 A CN201611190863 A CN 201611190863A CN 106509332 A CN106509332 A CN 106509332A
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preparation
sillver
fish
soft gel
gel
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CN106509332B (en
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高瑞昌
石彤
袁丽
王艳敏
赵梦琴
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Jiangsu University
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Jiangsu University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/04Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

The invention discloses a preparation method for silver carp meat protein soft gel and relates to the technical field of the quality improvement of aquatic products. The magnitude of breaking force serves as the standard for judging the strength of the gel, detection results are compared with detection data of a blank sample according to changes, caused by adding lysine, of the strength of the silver carp meat protein soft gel, and thus whether the lysine added silver carp meat protein soft gel is soft gel or not is judged. The high-protein and high-nutrition silver carp meat protein soft gel easy to eat and suitable for special people such as the old and patients is prepared.

Description

A kind of preparation method of the soft gel of sillver carp proteins
Technical field
The present invention relates to aquatic products quality-improving technical field, more particularly to the soft gel system of high-quality Hypophthalmichthys molitrix high concentration albumen The production of product.
Background technology
Fish product mouthfeel is soft, easy to digest, nutritious, and has many dietetic therapy effects, blue or green by consumers in general Look at.But as general minced fish gellike product belongs to high salt product, the consumption main force is vast young consumers, which is higher to contain Salt amount and stronger elasticity, chewiness are difficult to the special populations such as suitable old people, patient and eat.Many Zhou Suozhi, minced fish albumen system The preparation of product be fish protein salt it is molten after gel is formed by thermal induction method, if reducing the salinity in product, can be because of Fish protein it is insoluble without formed gel.Therefore in order to the less salt gel for obtaining high concentration albumen is then needed by addition Different additive is improving the dissolubility of albumen.Research at present focuses primarily upon CaCl2、MgCl2, phosphate etc. is to fish protein The impact of physicochemical property, and research and application that aminoacid is affected on sillver carp proteins Gelatin Quality then have no report.The present invention The angle changing of the fish protein gelling value performance caused from lysine is cut, is objectively commented according to the size of gel strength Impact of the valency lysine to fish protein Gelatin Quality, the preparation to oppress soft gel provide feasible theoretical foundation, by this Method can obtain high protein, high nutrition, the easily edible soft gel product of the flesh of fish, meet in the special groups such as patient, old people Demand.
The content of the invention
The angle changing incision of the fish protein gelling value performance that the present invention causes from lysine, according to gel strength Size objectively evaluate impact of the lysine to fish protein Gelatin Quality.
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Frozen centrifugation is carried out, fish protein solution is finally given, 4 DEG C of refrigerators are placed on, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 1~50mM.The each sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, place in 4 DEG C of refrigerators 12h, it is standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) it is last using the size of Fracture Force as the standard for passing judgment on gelling value performance, sillver carp proteins are evaluated with this and is coagulated The intensity size of glue, according to testing result compared with blank sample detection data, so as to evaluate the Hypophthalmichthys molitrix meat of addition lysine Whether protein gel is soft gel.
Wherein described the step of (3), is specifically carried out as steps described below:Fish protein solution after extraction is dispensed into into 15 In individual small beaker, it is one group per three, while adding lysine so that the final concentration of 1~50mM of lysine.By above-mentioned preparation Each sample carry out two-period form heating (first 60min is heated at 40 DEG C, then heat 30min at 90 DEG C), be then placed in frozen water In cool down rapidly, 12h is placed in 4 DEG C of refrigerators, it is standby.
This method result shows:With not plus lysine fresh fish protein compared with, when lysine addition 1mM~ When changing between 50mM, there is different degrees of change in gel strength.After this is possibly due to add lysine, on the one hand, band The lysine of positive electricity can be combined with electronegative fish protein, so that the chance be combineding with each other between fish protein is reduced, The aggregation of fish protein is inhibited to a certain extent;On the other hand, after adding lysine, the pH value of solution increased, and make The electrostatic repulsion obtained between fish protein strengthens, and this can also suppress protein aggregation, so that the gel strength for ultimately forming subtracts It is weak.As the addition of lysine is different, lysine is different from the change degree of the combination degree of fish protein and solution ph, So that under different lysine additions, the gel strength for measuring is also different.
Specific embodiment
Comparative example:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:The fish protein solution of extraction is carried out into two-period form heating, then rapidly Cooling, places 12h in 4 DEG C of refrigerators, standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 52.7 ± 1.13g, and gel strength is stronger.
Embodiment 1:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 1mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, it is standby With;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 19.6 ± 0.72g, and gel strength is compared with comparative example significance decrease.
Embodiment 2:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 5mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, it is standby With;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 17.5 ± 0.62g, and gel strength is compared with comparative example significance decrease.
Embodiment 3:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 10mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, It is standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 15.1 ± 0.87g, and gel strength is compared with comparative example significance decrease.
Embodiment 4:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 20mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, It is standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 18.9 ± 1.13g, and gel strength is compared with comparative example significance decrease.
Embodiment 5:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 30mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, It is standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 16.1 ± 0.55g, and gel strength is compared with comparative example significance decrease.
Embodiment 6:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 40mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, It is standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 17.3 ± 1.04g, and gel strength is compared with comparative example significance decrease.
Embodiment 7:
The preparation of the cleaning for including the flesh of fish of the invention, peeling, the extraction of fish protein, the measure of protein content and sample, The programs such as texture properties measure, data processing, concrete production technology are as follows:
The preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of fish protein:Corresponding solution and reagent are sequentially added on request in the back flesh of fish of minced fish shape Extracted, finally given fish protein solution, be placed on 4 DEG C of refrigerators, it is standby;
(3) preparation of fish protein gel sample:Add lysine in the fish protein solution for extracting so that lysine Concentration be 50mM.The sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, 12h is placed in 4 DEG C of refrigerators, It is standby;
(4) texture properties are determined:Instrument is the production of Stable Micro Systems companies of Britain TA.XT.Plus physical properties of food instrument, chooses P/0.5 cylinder probes, carries out pressure test, and it is 1.5mm/s to arrange preparation speed;Survey Examination speed is 1.0mm/s;After degree of testing the speed be 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed;
(5) Jing is determined, and the Fracture Force of this group of sample is 16.2 ± 1.01g, and gel strength is compared with comparative example significance decrease.

Claims (6)

1. the preparation method of the soft gel of a kind of sillver carp proteins, it is characterised in that carry out as steps described below:
(1) cleaning oppressed, peeling:Aristichthys nobiliss are rinsed well with clear water, the back flesh of fish, cleaning, peeling is chosen;
(2) extraction of sillver carp proteins:Corresponding solution is sequentially added on request in the back flesh of fish of minced fish shape and reagent enters Row frozen centrifugation, finally gives sillver carp proteins solution, is placed on 4 DEG C of refrigerators, standby;
(3) preparation of sillver carp proteins gel sample:Add lysine in the sillver carp proteins solution for extracting so that lysine Concentration be 1~50mM, each sample of above-mentioned preparation is carried out into two-period form heating, it is then rapid to cool down, place in 4 DEG C of refrigerators 12h, it is standby;
(4) texture properties are determined:
(5) it is last using the size of Fracture Force as the standard for passing judgment on gelling value performance, sillver carp proteins gel is evaluated with this Intensity size, according to testing result compared with blank sample detection data, so as to evaluate the sillver carp proteins of addition lysine Whether gel is soft gel.
2. the preparation method of the soft gel of a kind of sillver carp proteins according to claim 1, it is characterised in that wherein described Step (3) is specifically carried out as steps described below:Sillver carp proteins solution after extraction is dispensed in 15 small beakers, per three For one group, while adding lysine so that the final concentration of 1~50mM of lysine.
3. the preparation method of the soft gel of a kind of sillver carp proteins according to claim 1, it is characterised in that wherein described Step (3) is specifically carried out as steps described below:The each sample of above-mentioned preparation is carried out into the two-period form heating i.e. first heating at 40 DEG C 60min, then 30min is heated at 90 DEG C, cooled down in being then placed in frozen water rapidly, 12h is placed in 4 DEG C of refrigerators, it is standby.
4. the preparation method of the soft gel of a kind of sillver carp proteins according to claim 1, it is characterised in that wherein described Step (4) instrument is the TA.XT.Plus physical properties of food instrument of Stable Micro Systems companies of Britain production, is chosen P/0.5 cylinder probes, carry out pressure test.
5. the preparation method of the soft gel of a kind of sillver carp proteins according to claim 1, it is characterised in that wherein described It is 1.5mm/s that step (4) arranges preparation speed;Test speed is 1.0mm/s.
6. the preparation method of the soft gel of a kind of sillver carp proteins according to claim 1, it is characterised in that wherein described After step (4), degree of testing the speed is 1.0mm/s;Distance is 4mm;Trigger force is 4g.Fracture Force (g) after recording compressed.
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CN109673811A (en) * 2019-01-16 2019-04-26 江苏大学 A kind of method that high concentration myosin maintains liquid at high temperature
CN113598331A (en) * 2021-07-15 2021-11-05 江苏大学 Method for preparing snakehead-basic tail shrimp paste product
CN115152980A (en) * 2022-07-29 2022-10-11 陕西科技大学 Meat protein jelly and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN109673811A (en) * 2019-01-16 2019-04-26 江苏大学 A kind of method that high concentration myosin maintains liquid at high temperature
CN113598331A (en) * 2021-07-15 2021-11-05 江苏大学 Method for preparing snakehead-basic tail shrimp paste product
CN115152980A (en) * 2022-07-29 2022-10-11 陕西科技大学 Meat protein jelly and preparation method thereof

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