CN101711591B - Preparation method of fish cartilage extracts and obtained product - Google Patents
Preparation method of fish cartilage extracts and obtained product Download PDFInfo
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- CN101711591B CN101711591B CN2008102008816A CN200810200881A CN101711591B CN 101711591 B CN101711591 B CN 101711591B CN 2008102008816 A CN2008102008816 A CN 2008102008816A CN 200810200881 A CN200810200881 A CN 200810200881A CN 101711591 B CN101711591 B CN 101711591B
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Abstract
The invention discloses a preparation method of fish cartilage extracts and an obtained product, comprising the following steps: carrying out alkaline protease enzymolysis on fish cartilage to obtain enzymolysis liquid, carrying out flavorzyme enzymolysis, inactivating enzyme and removing impurities to obtain the fish cartilage extract. The invention overcomes the defects that the existing methodfor producing chondroitine and/ or collagen peptide can not fully utilize the raw materials, the product has low effective components, the raw materials are threatened by pollution or the steps are complex and the like. The invention provides the preparation method of fish cartilage extracts and the obtained product. The invention can acquire lacking products in the market by a simple technical process, further improves use value of the raw materials, not only can solve the problem of lacking collagen peptide in the market and chondroitine, but also change waste material into useful things, reasonably and fully utilize natural resources, make the best use of things and reduce environmental pollution; the invention has the advantages of low raw material cost, high product security and highcontent of effective components.
Description
Technical field
The present invention relates to a kind of preparation method of fish cartilage extracts and the product of gained.
Background technology
Collagen peptide is the main component in the human connective tissue, is skin, bone, tendon, the cartilage of human body, the constituent material of blood vessel, accounts for 1/3rd of body internal protein total amount.The protein digestibility absorptivity of collagen peptide almost reaches 100%; collagen peptide has the gastric mucosa of protection, promotes the skin collagen metabolism, suppresses increased blood pressure, promotes multiple efficacies such as calcium absorption and reduction cholesterol in serum content according to the literature; therefore collagen peptide is widely used in each field such as medicine, food, beauty treatment, Feed Manufacturing, chemical industry, is market type goods and materials in short supply.
Chondroitin sulfate generally is the natural acidic mucopolysaccharide that extracts from the animal cartilaginous tissue, has the height water conservation and keeps viscoplasticity, the lubrication in joint.In recent years, along with deepening continuously of research, find that chondroitin sulfate also has anti-inflammatory, antiviral and antitumor isoreactivity, be far superior to the chondroitin sulfate in pig, ox cartilage source from the chondroitin sulfate of fish cartilage.Particularly shark itself is through repeatedly check, and none routine cancer cell exists.With the chondroitin sulfate that Shark cartilage is produced, show clinically strong anticancer, control the cancer effect.Chondroitin sulfate has certain curative effect to the treatment of angiocardiopathy, especially the important source material of some health products and cosmetics.Chondroitin sulfate also has multiple biologically active, is extensive use of in a lot of fields.
The raw material of producing collagen peptide is mainly derived from lands such as ox, pig and chicken and produces animal.But along with in recent years, domestic animals and fowls class eqpidemic disease problems such as rabid ox disease, aftosa, bird flu take place in succession, add that some consumers use this type of collagen peptide that irritated tendency is arranged, and the security that collagen peptide is produced in the land is under suspicion.People begin extracting higher, the virus-free pollution of security and the harmless collagen peptide of health has had more concern from the skin of aquatic livestock, cartilage, fin, squama etc.Chondroitin sulfate can extract from Shark cartilage, fin, but cost is higher, and the fish scale raw material is difficult to obtain.China's fish output occupies first place in the world, and nearly 4,000 ten thousand tons of annual production is in general processing of aquatic products process, fish cartilage, a discarded fish Chang Zuowei leftover bits and pieces, or be processed into fishbone dust and make an addition in the poultry feed, but more fish cartilage, fish head be dropped, and causes environmental pollution and the wasting of resources.
At present, generally showed all that in some relevant patents from fish-bone enzyme process produces the industrial process of the former peptide of fish glue or extract the method for chondroitin sulfate from animal cartilage.But, all can only extract wherein a kind of composition, usually another composition can be ignored, cause huge waste.And the various complexity of operating procedure.
Summary of the invention
Technical problem to be solved by this invention is that to overcome the method for existing production chondroitin sulfate and/or collagen peptide insufficient to prepared using, product active ingredient is lower, and raw material has defectives such as pollution threat or step be numerous and diverse, and a kind of preparation method and products obtained therefrom of fish cartilage extracts is provided.The present invention can not only solve the problem of market collagen peptide in short supply and chondroitin sulfate, and real makes a silk purse out of a sow's ear, and rationally makes full use of the natural resources, and accomplishes to make the best use of everything, and reduces environmental pollution; And cost of material is cheap, product safety performance height, active constituent content height.
The preparation method of fish cartilage extracts of the present invention comprises the steps: the fish cartilage is carried out the alkali protease enzymolysis, obtains enzymolysis liquid, carries out the flavor protease enzymolysis afterwards again, and go out then enzyme, impurity elimination namely get fish cartilage extracts.Described fish cartilage extracts contains chondroitin sulfate, Isin glue collagen peptide and water.
Below, further the preparation method to fish cartilage extracts of the present invention describes in detail:
(1) pretreatment of raw material: described fish cartilage can be made by following method: with the fish cartilage raw material of cleaning, the heat treated impurity elimination, get cartilage.Wherein, described fish cartilage raw material be the conventional aquatic products that use in this area contain the cartilage position, preferable in salmon (Oncorhynchnchus Spp.), hole ray (Rajaporosa) and the shark (prionace glauca (Linnaeus)) one or more contain the cartilage position; What the heating-up temperature of described heat treated was preferable is 70 ℃～100 ℃, and that heat time heating time is preferable is 10～30min; The employed equipment of described heat treated is the conventional equipment that uses in this area, and preferable is retort, water-bath or pressure cooker.Described cartilage is mechanical disintegration further, helps the extraction of fish cartilage extracts, and the employed equipment of described mechanical disintegration is the conventional equipment that uses in this area, and preferable pulverizes homogenizer, ultrasonic wave or the fruit juice mixer of function for having physical damage.
(2) protease hydrolyzed
The alkali protease enzymolysis: the condition of described alkali protease enzymolysis is this area conventional steps condition, preferably adds water in the fish cartilage, after the accent pH alkalescence, adds the alkali protease enzymolysis.Wherein, the consumption of the described water that adds water is preferable is 0.8～2 times of fish cartilage weight; What described pH was preferable is 7.5～9.0; Described accent pH regulates with the conventional alkali that uses in this area, and preferable is NaOH; What the consumption of described alkali protease was preferable is 0.05～0.25% of fish cartilage weight; What the temperature of described alkali protease enzymolysis was preferable is 50～65 ℃, and that the time of alkali protease enzymolysis is preferable is 0.5～3h; Described alkali protease is the conventional alkali protease that uses in this area, and preferable is Alcalase3.0T or Alcalase2.4L alkali protease.
The flavor protease enzymolysis: the step of described flavor protease enzymolysis is this area conventional steps condition, preferably enzymolysis liquid is adjusted to pH acidity, adds the flavor protease enzymolysis.What wherein, described pH was preferable is 6.0～7.0; Described accent pH regulates with the conventional acid of using in this area, and preferable is hydrochloric acid; What the consumption of described flavor protease was preferable is 0.03%～0.25% of fish cartilage weight; What the temperature of described flavor protease enzymolysis was preferable is 50～65 ℃, and that the time of flavor protease enzymolysis is preferable is 0.5～3h; Described flavor protease is the conventional flavor protease that uses in this area, and preferable is the Novo flavor protease.
Among the present invention, protease hydrolyzed is first alkali protease enzymolysis, and then the flavor protease enzymolysis.The enzymolysis of described alkali protease is the combination of cutting off mucopolysaccharide and albumen in the fish cartilage, and chondroitin sulfate enzymolysis from the albumen is got off, and makes chondroitin sulfate and Protein Separation, realization mucopolysaccharide and collagen initial gross separation; The effect of described flavor protease is that macromolecular collagen enzymolysis in the fish cartilage is become micromolecular collagen peptide, is dissolved in the enzymolysis liquid.The compound use of these two kinds of protease is ready for separating acquisition chondroitin sulfate and/or collagen peptide.
(3) go out enzyme, impurity elimination: the described enzyme that goes out, impurity elimination step are this area conventional steps condition, and preferred condition comprises as follows: stop heating after being warmed up to uniform temperature, and cooling, centrifugal, filter impurity elimination, namely get fish cartilage extracts.What wherein, the temperature of described intensification was preferable is 80～100 ℃; Described cooling preferable for being cooled to below 75 ℃; Described centrifugal preferable condition is: the centrifugal 15～40min of 2000～6000r/min; The process of described filtration is preferable can add an amount of filter aid white bole or diatomite helps filter.
The prepared fish cartilage extracts of the present invention contains chondroitin sulfate, Isin glue collagen peptide and water.Wherein, described Isin glue collagen peptide is that a kind of hydroxyproline, glycine and proline content account for more than 35%, and molecular weight is 206～400 daltonian micromolecular collagen peptides; Described chondroitin sulfate is that glycosyl is glucuronic acid and acetylamino galactosamine, and molecular weight is 5000～15000 daltonian chondroitin sulfates.
The present invention can also cross the above-mentioned fish cartilage extracts that makes the membrane molecule sieve and carry out the molecular weight cutting, further separates making Isin glue collagen peptide and/or chondroitin sulfate.
A preferred embodiment of the present invention: the above-mentioned fish cartilage extracts that makes is crossed the membrane molecule sieve carry out the molecular weight cutting, film sees through the liquid drying, namely makes Isin glue collagen peptide.
Another preferred embodiment of the present invention: the above-mentioned fish cartilage extracts that makes is crossed the membrane molecule sieve carry out the molecular weight cutting, film concentrate drying namely makes chondroitin sulfate.
The another preferred embodiment of the present invention: the above-mentioned fish cartilage extracts that makes is crossed the membrane molecule sieve carry out the molecular weight cutting, film sees through the liquid drying, namely makes Isin glue collagen peptide; Film concentrate drying namely makes chondroitin sulfate (technological process such as Fig. 1).
Below, further the step to above-mentioned preferred embodiment describes in detail:
(1) membrane molecule screening from: with filtrate thin up to 1.5～2.5 times filtrate quality, cross the biomembrane molecule sieve and carry out the molecular weight cutting, obtain film and see through liquid and film concentrate.Wherein, the operating condition of described molecular weight cutting preferable for temperature range is 30～70 ℃, pressure limit is preferable is to carry out the molecular weight cutting, circulation separation 0.5～2h under the condition of 1.5～7 kg/cm.The biomembrane molecule sieve that described membrane molecule screening is used with this area, preferable molecular cut off is 5000～15000 daltonian rolled films, tubular film or hollow-fibre membrane.
(2) film with gained is dried to little molecule Isin glue collagen peptide powder through liquid; Gained film concentrate drying is made the chondroitin sulfate powder.Wherein, described drying mode is the conventional drying mode that uses in this area, and preferable is spray-drying or freeze drying.
Each above-mentioned technical characterictic of the present invention can any combination, obtains preferable technical scheme and is used for preparing Isin glue collagen peptide and/or chondroitin sulfate by the fish cartilage.
The prepared product Isin glue collagen peptide of the present invention is analyzed through the amino acid analysis method, for a kind of hydroxyproline, glycine and proline content account for more than 35%, the collagen peptide content reaches more than 72%, and molecular weight is about 206～400 daltonian micromolecular collagen peptides; It is glucuronic acid and acetylamino galactosamine that the chondroitin sulfate that makes records glycosyl through the trifluoroacetic acid hydrolysis method, and sulfuric acid-carbazole method detects chondroitin sulfate purity and reaches 40%～90%, and its molecular weight is 5000～15000 dalton; Percentage is mass percent.Wherein, sulfuric acid-carbazole ratio juris is that the molecular weight according to chondroitin sulfate is 503.3, the molecular weight of glucuronic acid is 194.1, be coefficient with 503.3/194.1=2.593, record light absorption value in the 530nm place, calculate the content of glucuronic acid, multiply by coefficient 2.593 again, namely get the content of chondroitin sulfate.
Agents useful for same of the present invention and raw material be commercially available getting all.
Positive progressive effect of the present invention is:
1. the present invention is by making fish cartilage extracts with the fish cartilage through the enzymolysis of complex enzyme solution, and several preferred embodiments take full advantage of membrane molecule sieve element amount cutting technique simultaneously, have prepared collagen peptide and/or chondroitin sulfate.It is insufficient that the present invention has overcome the prepared using of existing production collagen peptide and/or chondroitin sulfate, product active ingredient is lower, and having avoided utilizing lands such as pig, ox cartilage to produce the animal cartilage raw material has popular eqpidemic disease to cause the threat of unsafe factor, use defectives such as a large amount of organic reagent contaminated environment or step are numerous and diverse, utilize two kinds of protease hydrolyzeds, further improved prepared using value, from a simple technical flow, obtain market product in short supply, solve the problem of market collagen peptide in short supply and chondroitin sulfate.Raw material used in the present invention is real making a silk purse out of a sow's ear for discarded fish cartilage simultaneously, rationally makes full use of the natural resources, and accomplishes to make the best use of everything, and reduce environmental pollution, and cost of material is cheap.
2. the prepared Isin glue collagen peptide of the present invention is the little molecule Isin glue collagen peptide of a kind of high-recovery, high permeability, produces animal collagen compared to the land, and molecular weight is little, and toughness is also better; The chondroitin sulfate that the present invention makes can be applicable to various aspects such as medical material, health food, normal food, cosmetics.
Description of drawings
Fig. 1 is the preparation chondroitin sulfate of a preferred embodiment of the present invention and the process chart of Isin glue collagen.
The specific embodiment
Mode below by embodiment further specifies the present invention, but does not therefore limit the present invention among the described scope of embodiments.
Among the following embodiment, prepared Isin glue collagen peptide detects the content (mass percent) of collagen peptide purity (total amino acid content) and hydroxyproline, glycine and proline by the amino acid analysis method; Prepared chondroitin sulfate detects glycosyl by the trifluoroacetic acid hydrolysis method, and carbazole-sulfuric acid process records its purity, and the Ubbelohde viscosimetry is surveyed molecular weight.
Embodiment 1
It is an amount of to get clean salmon (Oncorhynchnchus Spp.) head, 100 ℃ of heating 10min in retort, isolation of cartilage 500g pulverizes with homogenizer, add 500g water, transferring pH with 10% NaOH is 8.5, add the Alcalase3.0T alkali protease of raw material weight 0.1% in 65 ℃ of enzymolysis 1h, transferring pH with 1% hydrochloric acid is 6.5, adds the Novo flavor protease of raw material weight 0.05% in 65 ℃ of enzymolysis 2h.Be warming up to 95 ℃ of enzymes that go out, be cooled to 70 ℃, the centrifugal 30min of 4000r/min, filter impurity elimination, add the thick diatomite of 80g white and help filter, filtrate thin up to 2 times filtrate quality, cross molecular cut off and be 5000 rolled film molecular sieve, be to carry out the molecular weight cutting under the condition of 2 kg/cm at 50 ℃, pressure, circulation separates 1h, and it is the Isin glue collagen peptide powder that collection membrane sees through liquid decompression concentrated spray drying; The concentrate spray-drying is the chondroitin sulfate powder in the collection membrane.
The content that makes Isin glue collagen peptide hydroxyproline, glycine and proline is 35.3%, and the collagen peptide content is 72%, and molecular weight is 206 dalton; Chondroitin sulfate glycosyl after testing is glucuronic acid and acetylamino galactosamine, and molecular weight is 1.5 ten thousand dalton, and purity is 40%.
Embodiment 2
It is an amount of to get clean hole ray (Raja porosa), 85 ℃ of heating 20min impurity elimination in retort, isolation of cartilage 500g pulverizes with homogenizer, add 500g water, transferring pH with 10% NaOH is 7.5, add the Alcalase3.0T alkali protease of cartilage weight 0.2% in 55 ℃ of enzymolysis 0.5h, transferring pH with 1% hydrochloric acid is 6.0, adds the Novo flavor protease of cartilage weight 0.2% in 55 ℃ of enzymolysis 1h.Be warming up to 85 ℃ of enzymes that go out, be cooled to 50 ℃, the centrifugal 30min of 4000r/min, and filter impurity elimination, add the 80g white bole and help filter.2 times of filtrate quality of filtrate thin up, cross molecular cut off and be under the condition that 5000 tubular membrane molecular sieve is 2 kg/cm at 70 ℃, pressure and carry out the molecular weight cutting, circulation separates 1.5h, and it is the Isin glue collagen peptide powder that collection membrane sees through liquid decompression concentrated spray drying; Collection membrane concentrate, spray-drying are the chondroitin sulfate powder.
The content that makes Isin glue collagen peptide hydroxyproline, glycine and proline is 36.2%, and the collagen peptide content is 85%, and molecular weight is 350 dalton; The chondroitin sulfate that makes glycosyl after testing is glucuronic acid and acetylamino galactosamine, and molecular weight is 1.1 ten thousand dalton, and purity is 70%.
Embodiment 3
It is an amount of to get clean shark (prionace glauca (Linnaeus)) bone, 75 ℃ of heating 30min impurity elimination in water-bath, separating the 500g cartilage pulverizes with homogenizer, add 600g water, transferring pH with 10% NaOH is 9, add the Alcalase2.4L alkali protease of raw material weight 0.25% in 50 ℃ of enzymolysis 0.5h, transferring pH with 1% hydrochloric acid is 6.8, adds the Novo flavor protease of raw material weight 0.25% in 50 ℃ of enzymolysis 0.5h.Be warming up to 80 ℃ of enzymes that go out, be cooled to 45 ℃, 2000r/min is centrifugal, and 40min is centrifugal, filter impurity elimination, and add 80g diatomite and help filter, 2 times of filtrate quality of filtrate thin up are crossed molecular cut off and are 15000 hollow-fibre membrane molecular sieve and carry out the molecular weight cutting being lower than under the condition that 50 ℃, pressure are 1.5 kg/cm, circulation separates 2h, and it is the Isin glue collagen peptide powder that collection membrane sees through liquid decompression concentrated spray drying; Collection membrane concentrate, spray-drying are the chondroitin sulfate powder.
Make the content of Isin glue collagen peptide hydroxyproline, glycine and proline 35%, the collagen peptide content is 83%, and molecular weight is 315 dalton; Chondroitin sulfate glycosyl after testing is glucuronic acid and acetylamino galactosamine, and molecular weight is 1.2 ten thousand dalton, and purity is 90%.
Embodiment 4
It is an amount of to get clean salmon (Oncorhynchnchus Spp.) head, 70 ℃ of heating 30min in retort, isolation of cartilage 500g pulverizes with homogenizer, add 400g water, transferring pH with 10% NaOH is 8.5, add the Alcalase3.0T alkali protease of raw material weight 0.05% in 50 ℃ of enzymolysis 3h, transferring pH with 1% hydrochloric acid is 7.0, adds the Novo flavor protease of raw material weight 0.03% in 55 ℃ of enzymolysis 3h.Be warming up to 100 ℃ of enzymes that go out, be cooled to 75 ℃, the centrifugal 15min of 6000r/min filters impurity elimination, adds the thick diatomite of 80g white and helps filter, obtains fish cartilage extracts.The fish cartilage extracts that makes contains Isin glue collagen peptide, chondroitin sulfate and water.The molecular weight of Isin glue collagen peptide is 306 dalton, and the content of hydroxyproline, glycine and proline is more than 35%; The glycosyl of chondroitin sulfate is glucuronic acid and acetylamino galactosamine, and molecular weight is 5000 dalton; Percentage is mass percent.
Embodiment 5
It is an amount of to get clean salmon head (OncorhynchnchusSpp.) and shark (prionaceglauca (Linnaeus)) bone, 100 ℃ of heating 10min in retort, isolation of cartilage 500g pulverizes with homogenizer, add 1000g water, transferring pH with 10% NaOH is 8.5, add the Alcalase3.0T alkali protease of raw material weight 0.15% in 65 ℃ of enzymolysis 2h, transferring pH with 1% hydrochloric acid is 6.5, adds the Novo flavor protease of raw material weight 0.15% in 65 ℃ of enzymolysis 1.5h.Be warming up to 95 ℃ of enzymes that go out, be cooled to 70 ℃, the centrifugal 30min of 4000r/min, filter impurity elimination, 1.5 times of filtrate quality of filtrate thin up are crossed molecular cut off and are 8000 rolled film molecular sieve, at 30 ℃, pressure are to carry out the molecular weight cutting under the condition of 7 kg/cm, circulation separates 0.5h, and it is the Isin glue collagen peptide powder that collection membrane sees through liquid decompression concentrated frozen drying.
The content that makes Isin glue collagen peptide hydroxyproline, glycine and proline is 35%, and the collagen peptide content is 75%, and molecular weight is 400 dalton.
Embodiment 6
It is an amount of to get clean hole ray (Raja porosa), 85 ℃ of heating 20min impurity elimination in retort, isolation of cartilage 500g, add 750g water, transferring pH with 10% NaOH is 7.5, add the Alcalase3.0T alkali protease of cartilage weight 0.2% in 55 ℃ of enzymolysis 0.5h, transferring pH with 1% hydrochloric acid is 6.0, adds the Novo flavor protease of cartilage weight 0.2% in 55 ℃ of enzymolysis 2.5h.Be warming up to 85 ℃ of enzymes that go out, be cooled to 50 ℃, the centrifugal 30min of 4000r/min, and filter impurity elimination, add the 80g white bole and help filter.2.5 times of filtrate quality of filtrate thin up, cross molecular cut off and be 5000 tubular film molecular sieve and carry out the molecular weight cutting being lower than under the condition that 70 ℃, pressure are 7.0 kg/cm, circulation separates 0.5h, the collection membrane concentrate, and spray-drying is the chondroitin sulfate powder.
The chondroitin sulfate that makes glycosyl after testing is glucuronic acid and acetylamino galactosamine, and molecular weight is 9000 dalton, and purity is 80%.
In sum, fish cartilage micromolecular collagen peptide and chondroitin sulfate that the inventive method makes have been saved enterprise in the burden of discharging that disposes waste liquid when no a large amount of organic reagents pollute; The collagen peptide that the inventive method technological process makes and chondroitin sulfate purity height, quality safety, and improved the value of discarded fish cartilage.
Claims (14)
1. the preparation method of a fish cartilage extracts, its step comprises carries out the alkali protease enzymolysis with the fish cartilage, obtains enzymolysis liquid, carries out the flavor protease enzymolysis afterwards again, and go out then enzyme, impurity elimination namely get fish cartilage extracts; The condition of described alkali protease enzymolysis after the accent pH alkalescence, is added the alkali protease enzymolysis for to add water in the fish cartilage; The step of described flavor protease enzymolysis is added the flavor protease enzymolysis for enzymolysis liquid is adjusted to pH acidity; Described fish cartilage extracts contains chondroitin sulfate, Isin glue collagen peptide and water;
Described fish cartilage extracts is crossed the membrane molecule sieve carry out the molecular weight cutting, the desciccator diaphragm concentrate namely makes chondroitin sulfate; Perhaps, described fish cartilage extracts is crossed the membrane molecule sieve carry out the molecular weight cutting, desciccator diaphragm sees through liquid, namely makes Isin glue collagen peptide, and the desciccator diaphragm concentrate namely makes chondroitin sulfate.
2. the method for claim 1, it is characterized in that: described fish cartilage is made by following processing method: one or more the cartilage position that contains in salmon, hole ray and the shark is cleaned, in 70 ℃～100 ℃ heat treated 10～30min, impurity elimination, get cartilage.
3. the method for claim 1, it is characterized in that: the step of described alkali protease enzymolysis is: the water that adds 0.8～2 times of its weight in the fish cartilage, after transferring pH7.5～9.0, add the alkali protease of fish cartilage weight 0.05%～0.25%, enzymolysis 0.5～3h in 50～65 ℃ of scopes obtains enzymolysis liquid.
4. as claim 1 or 3 described methods, it is characterized in that: described alkali protease is Alcalase3.0T or Alcalase2.4L alkali protease.
5. the method for claim 1, it is characterized in that: the step of described flavor protease enzymolysis is: enzymolysis liquid is adjusted to pH6.0～7.0, adds the flavor protease of fish cartilage weight 0.03%～0.25%, in 50 ℃～65 ℃ following enzymolysis 0.5～3h.
6. as claim 1 or 5 described methods, it is characterized in that: described flavor protease is the Novo flavor protease.
7. the method for claim 1, it is characterized in that: the step condition of the described enzyme that goes out, impurity elimination is: be warming up to 80～100 ℃ of enzymes that go out, be cooled to below 75 ℃ again after, with the centrifugal 15～40min of 2000～6000r/min, filter.
8. method as claimed in claim 7 is characterized in that: the process of described filtration is added the filter aid white bole or diatomite helps filter.
9. the method for claim 1, it is characterized in that: described membrane molecule sieve carries out the molecular weight step of cutting and is: with filtrate thin up to 1.5～2.5 times filtrate quality, cross the biomembrane molecule sieve and carry out the molecular weight cutting, obtain film and see through liquid and film concentrate.
10. method as claimed in claim 9 is characterized in that: the operating condition of described molecular weight cutting is, temperature range is 30～70 ℃, and pressure limit is to carry out the molecular weight cutting under the condition of 1.5～7 kg/cm, and circulation separates 0.5～2h; Described membrane molecule screening is selected as tubular film, rolled film or hollow-fibre membrane, and the molecular cut off of described membrane molecule sieve is 5000～15000 dalton.
11. the method for claim 1 is characterized in that: described drying mode is spray-drying or freeze drying.
12. the fish cartilage extracts that the method for claim 1 makes, it is characterized in that: the Isin glue collagen peptide in the described fish cartilage extracts is that a kind of hydroxyproline, glycine and proline content account for more than 35%, and molecular weight is 206～400 daltonian micromolecular collagen peptides; The glycosyl of chondroitin sulfate is glucuronic acid and acetylamino galactosamine in the described fish cartilage extracts, and molecular weight is 5000～15000 dalton; Percentage is mass percent.
13. the Isin glue collagen peptide that the method for claim 1 makes, it is characterized in that: described Isin glue collagen peptide is that a kind of hydroxyproline, glycine and proline content account for more than 35%, the collagen peptide content reaches more than 72%, molecular weight is 206～400 daltonian micromolecular collagen peptides, and percentage is mass percent.
14. the chondroitin sulfate that the method for claim 1 makes, it is characterized in that: the glycosyl of described chondroitin sulfate is glucuronic acid and acetylamino galactosamine, molecular weight is 5000～15000 dalton, and purity is 40%～90%, and percentage is mass percent.
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