CN102994602B - Preparation method of compound oligopeptide with high F value - Google Patents
Preparation method of compound oligopeptide with high F value Download PDFInfo
- Publication number
- CN102994602B CN102994602B CN201210557402.2A CN201210557402A CN102994602B CN 102994602 B CN102994602 B CN 102994602B CN 201210557402 A CN201210557402 A CN 201210557402A CN 102994602 B CN102994602 B CN 102994602B
- Authority
- CN
- China
- Prior art keywords
- oligopeptide
- enzymolysis
- preparation
- value
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a preparation method of compound oligopeptide with high F value. The method comprises the following steps of: performing smashing and extruding pretreatment on a mixed oil material with amino acid ratio balance and fatty acid ratio balance, extracting mixed liquor of oil and oligopeptide by selecting a two-step enzyme hydrolysis method, and carrying out dialysis, ultrafiltration and freeze-drying to obtain the oligopeptide with high F value by virtue of the absorption function of activated carbon. After the mixed oil material is adopted, the oligopeptide with amino acid ratio balance can be preferably obtained; and after the enzymic method is applied, the use of solvent can be reduced, the environment pollution and the wastewater treating energy consumption can be reduced, the environmentally-friendly preparation of the extracted mixed oil material oligopeptide can be guaranteed, and the protein is shorn according to the requirement of a human body to obtain the natural plant oligopeptide with high nutritional value.
Description
Technical field
The invention belongs to plant deep process technology field, be specifically related to the preparation method of the compound oligopeptide of a kind of high F value.
Background technology
High F value oligopeptide is a kind ofly to have the molecular-weight average of high branched-chain amino acid content and low die aromatischen Aminosaeuren content composition in the little peptide system of 300 ~ 1000D left and right.Studies have shown that in a large number, high F value oligopeptide has unique amino acid composition and physiological function, has assisting therapy or alleviates hepatogenic encephalopathy, improves effect such as proteinaceous nutrient situation, antifatigue of liver patient.Mainly concentrate on from single raw material and produce about the research of high F value oligopeptide both at home and abroad, such as soybean protein, fish-protein, casein, zein, sunflower protein etc., but have shortcoming more or less, as serious in material protein sex change, must aminoacids content imbalance, F value is low etc.
In soybean protein, the sulfur amino acid content such as methionine(Met), (partly) Gelucystine, Threonine is less, and peanut, linseed oil and tea seed have made up this deficiency, and under the effect of enzyme, the peptide molecular weight that proteolysis obtains is less, is easy to the formation of oligopeptide.
Raw material natural nuisance-free used in the present invention, derive from the seed of plant, after pulverizing through shelling, mix according to a certain percentage modified rear employing expelling-expansion pretreatment mode, through two step enzyme method processing, not only protein and peptide chain is cut into small peptide, and small peptide end is die aromatischen Aminosaeuren, can removes through charcoal absorption, obtain the mixing oil of fatty acid ratio balance simultaneously, can further make edible blend oil through refining.
Summary of the invention
The invention provides a kind of novel high F value oligopeptide technology of preparing, first the soybean of pulverizing, peanut, linseed oil, tea seed are mixed according to a certain percentage, after modified, carry out expelling-expansion pretreatment, after further pulverizing, carry out two step enzymolysis, grease in one step enzymolysis separate raw materials, two step enzymolysis make protein and peptide chain cut into needed small peptide, then remove die aromatischen Aminosaeuren and free fatty acids through charcoal absorption, further ultrafiltration, lyophilize can obtain the compound oligopeptide of high F value.
Step of the present invention is as follows:
Step 1: by the pulverizing of shelling respectively of soybean, peanut, linseed oil, tea seed, the material after pulverizing mixes for the ratio of 1:1:1:1 in mass ratio, regulates moisture content of material to 14%, then carries out expelling-expansion pretreatment to obtain expanded material;
Step 2: to crossing 80 mesh sieves after the expanded crushing material in step 1, expanded material and water after pulverizing and sieving are mixed to get mixed solution, described amount of water is 5:1 with quality of material ratio, obtain enzymolysis solution I to adding Alcalase2.4L Sumizyme MP to carry out enzymolysis in mixed solution, described enzyme concentration is the 1-3% of substrate mass concentration, enzymolysis pH is 7.5-9.5, hydrolysis temperature is 40-60 DEG C, enzymolysis time is 1-4h, then the enzyme that enzymolysis solution I heating 10min gone out, after the enzyme that goes out, centrifugation obtains mixing oil, polypeptide liquid and residue;
Step 3: by the polypeptide liquid of gained in step 2, add papoid to carry out enzymolysis and obtain enzymolysis solution II, described enzyme concentration is the 0.5-2% of substrate mass concentration, enzymolysis pH is 5-9, hydrolysis temperature is 40-60 DEG C, enzymolysis time is 0.5-2h, the enzyme that then enzymolysis solution II heating 5min gone out, and after the enzyme that goes out, centrifugation obtains supernatant liquor;
Step 4: obtain conjugated protein high F value oligopeptide through dialysis, ultrafiltration, lyophilize respectively after adding gac to remove die aromatischen Aminosaeuren and total free aminoacids the supernatant liquor of gained in step 3.
In described step 1, extruding puffing condition is 90 DEG C of sleeve temperature, screw speed 100rpm, nib aperture 15mm.
In described step 4 the addition of gac be substrate quality 5-10 doubly.
In described step 4, dialyse dialysis tubing molecular weight cut-off used below 3500Da.
In described step 4, ultra-filtration membrane aperture is 0.1-5 micron, and molecular weight cut-off is at 100-1000, and operation pressure reduction is at 0.5-3Mpa.
In described step 4, lyophilize condition is-50 DEG C of dry 12h under vacuum condition.
Useful point of the present invention is: the present invention adopts the preparation method of the compound oligopeptide of a kind of high F value, first the soybean of pulverizing, peanut, linseed oil, tea seed are mixed according to a certain percentage, after modified, carry out expelling-expansion pretreatment, after further pulverizing, carry out two step enzymolysis, grease in one step enzymolysis separate raw materials, two step enzymolysis make protein and peptide chain cut into needed small peptide, then remove die aromatischen Aminosaeuren and free fatty acids through charcoal absorption, further ultrafiltration, lyophilize can obtain high F value oligopeptide powder.Raw material sources are natural, and processing mode moderate notoxic is nuisanceless, are a kind of preparation methods of environmental protection.The F value of the high F value oligopeptide that the present invention obtains can reach 60-78, and free aminoacid content is 4 ~ 6%, and die aromatischen Aminosaeuren content is lower than 1%, and it can be used for the multiclass special foods such as nutrition dietetic food, functional health care food and arsenic.
Brief description of the drawings
Accompanying drawing is the process route chart of the inventive method.
embodiment:
Embodiment one: the step of present embodiment is as follows:
Step 1: by the pulverizing of shelling respectively of soybean, peanut, linseed oil, tea seed, material after pulverizing is in mass ratio for the ratio of 1:1:1:1 is mixed, regulate moisture content of material to 14%, then 90 DEG C of sleeve temperature, screw speed 100rpm, under the condition of nib aperture 15mm, carry out expelling-expansion pretreatment and obtain expanded material, then expanded material is pulverized to rear mistake 80 orders;
Step 2: by step 1 collect sieve after expanded material put into enzymatic vessel, solid-liquid ratio according to 1:5 adds distilled water, at 40 DEG C, add 2mol/L NaOH and regulate pH=7.5,2% the Alcalase2.4L Sumizyme MP that is incorporated as substrate mass concentration, isothermal reaction 3h, obtains enzymolysis solution I, the enzymolysis solution I heating 10min enzyme that goes out, centrifugation obtains mixing oil, polypeptide liquid and residue;
Step 3: by the polypeptide liquid in step 2, regulate pH value=5, temperature is 40 DEG C, 0.5% the amount that is substrate mass concentration according to enzyme concentration adds papoid, and reaction 1h, obtains enzymolysis solution II, by the enzymolysis solution II heating 5min enzyme that goes out, high speed centrifugation is isolated supernatant liquor;
Step 4: add gac to remove die aromatischen Aminosaeuren and total free aminoacids the supernatant liquor of gained in step 3, the mass ratio of gac addition 1:5(and substrate), obtain high F value oligopeptide powder through dialysis desalting, ultrafiltration purification, lyophilize.
Specific implementation method two: this implementation method is hydrolysis temperature to be adjusted to 50 DEG C in step 2 with specific implementation method one difference, and pH value is 8, and enzyme addition is 2%, and other composition and step are identical with specific implementation method one;
Specific implementation method three: this implementation method is in step 3 with specific implementation method one difference, pH=6, hydrolysis temperature is 55 DEG C, enzyme addition is 1%, reaction times 2h.Other composition and step are identical with specific implementation method one;
Specific implementation method four: this implementation method is hydrolysis temperature to be adjusted to 50 DEG C in step 2 with one of specific implementation method one difference, and pH value is 8, and enzyme addition is 2%; This implementation method is enzymolysis pH=6 in step 3 with two of specific implementation method one difference, and temperature is 55 DEG C, and enzyme addition is 1%, reaction times 2h.Other composition and step are identical with specific implementation method one.
Claims (6)
1. a preparation method for the compound oligopeptide of high F value, is characterized in that, described method steps is as follows:
Step 1: by the pulverizing of shelling respectively of soybean, peanut, linseed oil, tea seed, the material after pulverizing mixes for the ratio of 1:1:1:1 in mass ratio, regulates moisture content of material to 14%, then carries out expelling-expansion pretreatment to obtain expanded material;
Step 2: to crossing 80 mesh sieves after the expanded crushing material in step 1, expanded material and water after pulverizing and sieving are mixed to get mixed solution, described amount of water is 5:1 with quality of material ratio, obtain enzymolysis solution I to adding Alcalase2.4L Sumizyme MP to carry out enzymolysis in mixed solution, described enzyme concentration is the 1-3% of substrate mass concentration, enzymolysis pH is 7.5-9.5, hydrolysis temperature is 40-60 DEG C, enzymolysis time is 1-4h, then the enzyme that enzymolysis solution I heating 10min gone out, after the enzyme that goes out, centrifugation obtains mixing oil, polypeptide liquid and residue;
Step 3: by the polypeptide liquid of gained in step 2, add papoid to carry out enzymolysis and obtain enzymolysis solution II, described enzyme concentration is the 0.5-2% of substrate mass concentration, enzymolysis pH is 5-9, hydrolysis temperature is 40-60 DEG C, enzymolysis time is 0.5-2h, the enzyme that then enzymolysis solution II heating 5min gone out, and after the enzyme that goes out, centrifugation obtains supernatant liquor;
Step 4: obtain the compound oligopeptide of high F value through dialysis, ultrafiltration, lyophilize respectively after the supernatant liquor of gained in step 3 is added to gac.
2. the preparation method of the compound oligopeptide of a kind of high F value according to claim 1, is characterized in that in described step 1, extruding puffing condition is 90 DEG C of sleeve temperature, screw speed 100rpm, nib aperture 15mm.
3. the preparation method of the compound oligopeptide of a kind of high F value according to claim 1, the addition that it is characterized in that gac in described step 4 is 5-10 times of substrate quality.
4. the preparation method of the compound oligopeptide of a kind of high F value according to claim 1, is characterized in that dialysing dialysis tubing molecular weight cut-off used below 3500Da in described step 4.
5. the preparation method of the compound oligopeptide of a kind of high F value according to claim 1, is characterized in that in described step 4, ultra-filtration membrane aperture is 0.1-5 micron, and molecular weight cut-off is at 100-1000, and operation pressure reduction is at 0.5-3Mpa.
6. the preparation method of the compound oligopeptide of a kind of high F value according to claim 1, is characterized in that in described step 4, lyophilize condition is-50 DEG C of dry 12h under vacuum condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210557402.2A CN102994602B (en) | 2012-12-20 | 2012-12-20 | Preparation method of compound oligopeptide with high F value |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210557402.2A CN102994602B (en) | 2012-12-20 | 2012-12-20 | Preparation method of compound oligopeptide with high F value |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102994602A CN102994602A (en) | 2013-03-27 |
| CN102994602B true CN102994602B (en) | 2014-07-09 |
Family
ID=47923709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210557402.2A Active CN102994602B (en) | 2012-12-20 | 2012-12-20 | Preparation method of compound oligopeptide with high F value |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102994602B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103757083B (en) * | 2014-01-07 | 2016-01-20 | 沈阳师范大学 | A kind of production method of high-F-value oligopeptide of peanut meal |
| CN103865971B (en) * | 2014-03-14 | 2017-02-22 | 中华全国供销合作总社杭州茶叶研究所 | Tea protein high Fischer value oligopeptide as well as preparation method and application thereof |
| CN104904988A (en) * | 2015-06-17 | 2015-09-16 | 山东省农业科学院农产品研究所 | Method for processing peanut protein powder with high polypeptide content |
| CN105648009A (en) * | 2016-02-23 | 2016-06-08 | 浙江海洋学院 | Preparation method of high-Fischer-ratio oligopeptide from shark meat |
| CN105925651B (en) * | 2016-07-08 | 2019-10-25 | 黑龙江省科学院大庆分院 | A kind of preparation method of Chinese fiber crops seed dregs of rice polypeptide |
| CN109280683B (en) * | 2017-08-15 | 2021-01-12 | 广东丸美生物技术股份有限公司 | Preparation method of composite plant hydrolyzed protein peptide |
| CN108866133B (en) * | 2018-07-12 | 2022-03-04 | 天津科技大学 | Method for producing wheat bran oligopeptide by virtue of synergistic high-density fermentation of wheat bran through differential pressure pretreatment |
| CN110915980B (en) * | 2019-11-28 | 2023-03-14 | 湖北瑞邦生物科技有限公司 | Sunflower head peptide powder, and preparation method and application thereof |
| CN112592950B (en) * | 2020-12-31 | 2023-07-04 | 南京工业大学 | Method for synthesizing soybean-based high F value oligopeptide |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2986764B2 (en) * | 1997-07-04 | 1999-12-06 | 明治乳業株式会社 | Method for preparing high-fischer-ratio peptide with low bitter amino acid composition close to human milk |
| CN1570127A (en) * | 2003-07-23 | 2005-01-26 | 东北农业大学 | High F value oligopeptide production method by corn protein enzymolysis |
-
2012
- 2012-12-20 CN CN201210557402.2A patent/CN102994602B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102994602A (en) | 2013-03-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102994602B (en) | Preparation method of compound oligopeptide with high F value | |
| CN104082520B (en) | A kind of low value marine fish Gly-His-Lys and its preparation method and application | |
| CN103992384B (en) | A kind of large yellow croaker fish bone collagen peptide and its production and use | |
| CN103843970B (en) | Production method for preparing ossein oligopeptide meal, bone oil and bone meal | |
| CN101643767A (en) | Method for preparing almond peptide from almond dregs | |
| CN103992385A (en) | Pseudosciaena crocea swim bladder antioxidant collagen peptide and preparation method and application thereof | |
| Zhang et al. | A short review on the research progress in alfalfa leaf protein separation technology | |
| CN103461648B (en) | Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae | |
| CN103082081B (en) | A kind of Yeast protein and method for making thereof, with this albumen be raw material food and method for making thereof | |
| CN101233937A (en) | Method for preparing loach selenium-rich active peptide nutrient solution or nutrition powder | |
| CN105779542A (en) | Method for preparing organic rapeseed polypeptide through enzyme method | |
| CN104397321A (en) | Method for preparing compound amino acid through extensive enzymatic hydrolysis of soybean protein | |
| CN105925652A (en) | Method for producing small peptides through bionic enzymatic hydrolysis | |
| CN102839047A (en) | Method for extracting fish oil and producing taurine seafood condiments by using hairtail and leftovers of hairtail | |
| CN103757083B (en) | A kind of production method of high-F-value oligopeptide of peanut meal | |
| CN106174436A (en) | A kind of preparation method of complex enzyme hydrolysis fishbone hormone | |
| CN106359839A (en) | Extraction method of oyster peptides | |
| CN103876163A (en) | Rana japonica meat composite protein powder and preparation method thereof | |
| CN101965897A (en) | Processing method for mussel isolated protein | |
| CN103059745A (en) | Preparation method of tilapia skin gelatin | |
| CN101485383A (en) | Method for preparing soybean sport polypeptide by enzyme method | |
| CN105018557A (en) | Preparation method of pumpkin seed meal small peptides | |
| CN105192244A (en) | Method for preparing snakegourd fruit seed polypeptides | |
| CN101703155A (en) | Preparation method and application of prawn non-specific immunity reinforcing agent oligopeptide | |
| CN104152517A (en) | Method for extracting short peptides from sea horses |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180105 Address after: 257000 Kenli Economic Development Zone, east of Huafeng Road, Kenli Economic Development Zone, Dongying City, Shandong Province, north of Honghong River Patentee after: Shandong Wonderful Industrial Group Co., Ltd. Address before: 150030 Xiangfang City, Harbin Province Wood Street, No. 59, No. Patentee before: Northeast Agricultural University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200214 Address after: 315000 No.12 Xingye Avenue, Ningbo Free Trade Zone, Ningbo City, Zhejiang Province Patentee after: Ningbo sobao protein Technology Co., Ltd Address before: 257000 Kenli Economic Development Zone, east of Huafeng Road, Kenli Economic Development Zone, Dongying City, Shandong Province, north of Honghong River Patentee before: Shandong Wonderful Industrial Group Co., Ltd. |