CN101485383A - Method for preparing soybean sport polypeptide by enzyme method - Google Patents
Method for preparing soybean sport polypeptide by enzyme method Download PDFInfo
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- CN101485383A CN101485383A CNA2009100712912A CN200910071291A CN101485383A CN 101485383 A CN101485383 A CN 101485383A CN A2009100712912 A CNA2009100712912 A CN A2009100712912A CN 200910071291 A CN200910071291 A CN 200910071291A CN 101485383 A CN101485383 A CN 101485383A
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Abstract
The invention provides a method for preparing soybean kinetic polypeptide by an enzyme process, which is a production process for preparing kinetic polypeptide by applying protease to hydrolyze soybean protein. The test uses isolated soybean protein as a raw material, and adopts the protease to hydrolyze the isolated soybean protein so as to prepare the soybean kinetic polypeptide. The production process comprises pretreatment of the raw material, enzymolysis, ultra-filtration and separation, and drying. The process is simple and suitable for industrialized production. The soybean kinetic polypeptide can be directly used, can also be used as food nutrient supplement, can also be used as an ingredient for sports healthy food and army food at the same time of improving food protein content, and develops a novel path for deep processing of soybean protein.
Description
(1) technical field
The present invention relates to functional food, be specifically related to a kind of soybean sport polypeptide preparation method.
(2) background technology
Modern nutriology discovers that polypeptide has specific ionization amino acid and absorbs fast, characteristics such as absorptivity is good, biological value height, is the active material with different physiological roles.Wherein, the antifatigue peptide has obvious raising human body ability to work, strengthens muscle content strength, keeps or improve the locomitivity of body, and eliminates tiredly fast, recovers rapidly and strengthens effect such as muscle power, helps to keep health under movement environment.Therefore, the antifatigue peptide can become a kind of important function foodstuff base material of crowds such as being engaged in muscle power, mentality, sports.
This active peptides has and absorbs fast, low consumption, is difficult for saturated characteristics.Thereby, can supplement the nutrients fast for the crowd of some special health such as athletics, brain worker, field worker, alleviate tired.It is reported to have the free radical of removing, anti-oxidant isoreactivity composition in the soya-bean polypeptides, the free radical that can suppress body endogenous movement generation accumulates in a large number, the destruction of reducing human erythrocyte oxidation haemolysis degree and suppressing liposome membrane.The soybean active polypeptide has remarkable facilitation to multiple microbial growths such as lactic acid bacteria, Bifidobacterium and saccharomycete, thereby can improve interior environment, endocrine effect, and this absorption and resisting fatigue to food nutrition has important promotion meaning.The soya-bean polypeptides solid beverage recovers to have application widely aspect sportsman's endurance in the U.S., Romania to promoting.The molecular weight of the only company exploitation of Japan is being widely used aspect functional beverage, the sport nutrition food at the Soybean Peptide goods of 2400Da～5000Da.
At present, the method and the approach of the production of biologically active peptide preparation have three kinds: the one, from natural organism, extract itself intrinsic various natural activity peptide matters; The 2nd, the biologically active peptide that can obtain to have various physiological functions by the approach of protein degradation; The 3rd, use synthetic method and prepare biologically active peptide.Exist biologically active peptide in the natural biological body with various physiological functions, therefore from the natural biological body, can extract biologically active peptide, but because biologically active peptide content in vivo is generally very low, and extraction cost height, a large amount of problems such as bringing toxicity to having organic solvent residual in the pollution of environment and the biologically active peptide that extracted that with an organic solvent also can cause, therefore, should not make in this way in the actual production.Though and synthetic method can be by the synthetic any active peptide of people's wish, restricting and developing because exist cost height, side reaction to reach problem such as residual toxic compounds more at present.At present the maximum of usefulness are enzyme hydrolysis methods, and enzyme can be hydrolyzed under the condition of gentleness, and the biologically active peptide that can obtain having particular physiological function by kind, control reaction time and the degree of hydrolysis of selecting enzyme.It is safe that enzymolysis protein matter is produced active peptide, and hydrolytic process is easy to control, thereby the preparation method of the active peptide of report was all enzymatic isolation method in recent years.Current application proteolysis legal system is equipped with the focus that biologically active peptide becomes domestic and international research, and becomes the researcher and developed one of main path of novel health food and base-material thereof.
At present the biologically active peptide based food is in oneself listing early of developed countries such as Japan, the U.S. and West Europe, and China still is in the starting developing stage, broad market prospect to the research and development of biologically active peptide.
Modern study shows, functional areas at the inner possibility of the polypeptide chain ubiquity of nutrient protein, selects suitable these polypeptide chains of protease hydrolytic, it might be discharged, thereby prepare the biologically active peptide with various functional characteristics.By enzyme solution, just might discharge the functional areas fragments of peptides of being contained in the protein, prepare biologically active peptide preparation with various physiologically active.Therefore, very active about protolysate and active peptide Products Development both at home and abroad.People have obtained remarkable progress aspect biologically active peptide in recent years, and more and more biologically active peptides are found and separate, and have determined 26S Proteasome Structure and Function.The continuous discovery of active peptide, and, its significance is arranged all in theory research and clinical practice undoubtedly to their structure, the further investigation of function and mechanism of.
Before external research to proteolysis starts from more than 100 year, protein hydrolysate is applied in the food industry, at that time acid-hydrolyzed vegetable protein hydrolyzate (HVP) was made an addition in the food as flavor enhancement from beginning in 1886.Research about proteolysis is on the machining functions characteristic how to improve protein subsequently, as water-soluble, emulsibility, foaming characteristic, stability (heat endurance, bin stability) and flavor characteristics etc.Based on the security of acid and alkali hydrolysis thing, the hydrolysis research of protein also progressively turns on the direction of enzymatic hydrolysis simultaneously.Yet, really make the enzymolysis research of protein become the reason of focus and focus, be that scientist finds that from the zymolyte of protein also separation and purification goes out to have certain physiologically active and the significant biologically active peptide of effect.In recent years, the research to protein digestion technology abroad mainly concentrates on four aspects: the one, and the enzymolysis process research to protein resource newly developed has superior machining functions characteristic product in the hope of obtaining, for food industry provides new additive and batching; The 2nd, by utilizing new hydrolysising protease or process route, prepare the peptide molecular weight concentrated hydrolysate that more distributes, for example people such as Manuela is by the improvement of hydrolysis process, and the peptide molecular weight of its lactalbumin hydrolysate distributes and concentrates on 7500Da～8000Da and 4000Da～4500Da two parts substantially; The 3rd, by the improvement of hydrolysis process, further improve the yield of biologically active peptide, and obtain biologically active peptide from different protein source with identical effect, for example, vasotonia converting enzyme inhibitor (ACE inhibiting factor), its protein sources has a lot, can obtain from animal protein (lactoprotein, hemalbumin, fish albumen etc.), also can prepare from vegetable protein (soybean protein, corn gluten protein etc.); The 4th, the research of new-type enzymolysis process for example, combines enzymolysis and membrane separation technique, forms the hydrolysis process of continous way; Applying immobilized enzyme is hydrolyzed etc.
The research that China utilizes the proteolysis legal system to be equipped with the biologically active peptide aspect is started late, research to proteolysis in early days mainly is based on the machining functions characteristic aspect of improving protein, and number of research projects all concentrates on vegetable proteins such as soybean protein, peanut protein.For example, several years ago at the bigger HVP of China's flavoring industry amount ratio, its method for hydrolysis utilizes acid-hydrolysis method to make, and the result directly causes in the protolysate noxious materials such as residual chlorine propyl alcohol, and the flavouring industry of China is caused bigger impact.Yet, deep development along with related scientific research work, also based on the progressively understanding to the various physiologically actives that biologically active peptide had, the domestic in recent years research to biologically active peptide is also paid much attention to production simultaneously, and its primary study object mainly is soybean protein, lactoprotein etc.China had carried out the production of soya-bean polypeptides and the research work of application in recent years in a large number, and had obtained certain progress at the aspects such as debitterize, desalination and separation and purification of the selection of protease, enzymolysis process Parameter Optimization, hydrolyzate.
(3) summary of the invention
The object of the present invention is to provide a kind of technology of preparing of soybean sport polypeptide, be expected to become a kind of important function foodstuff base material of being engaged in crowds such as muscle power, mentality, sports.
The object of the present invention is achieved like this: a kind of preparation method of method for preparing soybean polypeptide by utilizing ereyme, and method may further comprise the steps:
1. material liquid preliminary treatment
Selecting soybean isolate protein powder (protein content 〉=80～83%) for use is raw material, adopts 75 ℃～90 ℃ water, makes concentration and be 3%～6% enzymolysis substrate, after evenly stirring, 10～18min is handled in insulation, cool to 50 ℃～65 ℃ then, and regulator solution pH value is 7～9.5.
2. enzyme digestion reaction
Adding 5%～20% protease, under 50 ℃～65 ℃ condition, react 2～5h through in the pretreated material liquid.
3. ultra-filtration and separation
After enzyme digestion reaction finishes, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da.Operating pressure is 0.1～0.5MPa, and temperature is 4 ℃～8 ℃
4. spray-drying
Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 160 ℃～180 ℃ of inlet temperatures, 75 ℃～90 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa～5MPa.
5. adopt protease hydrolyzed to prepare sport polypeptide, the product yield is 60.0%～70.0%, and degree of hydrolysis is 14.0%～20.0%
The present invention has also measured protein content, the Na of different molecular weight peptide section in the soybean protein isolate enzymolysis polypeptide solution
+Concentration.The result shows protein content 84.0%～90.0%, Na
+Concentration is 7.50%～15.00%.When this experiment product polypeptide is applied to sports drink, if press protein content 0.5g/100mL conversion, Na
+Concentration meets Na in the sports drink then at 41.5～90.0mg/100mL
+(5～90mg/100mL, GB15266-2000), so this experiment polypeptide products does not need to carry out desalting processing to the concentration of regulation.
The home and abroad document is less to the method report that the soybean protein isolate enzymolysis prepares soybean sport polypeptide; And a patent " enzyme process prepares the method for soya-bean polypeptides " for preparing soya-bean polypeptides about enzyme process only arranged at present." enzyme process prepares the method for soya-bean polypeptides " usefulness papain, composite flavor protease complex enzyme hydrolysis FSPC, through PS film ultra-filtration and separation device (molecular weight 6000Da dams) ultrafiltration, the product yield is 70.5%, and the following protein of molecular weight 5000Da accounts for 90% of total protein.
The molecular weight that the inventive method is produced is that the soya-bean polypeptides of 1000Da～5000Da has significant facilitation to mouse movement (mouse after feeding in 30 days, compare body weight with control group and improve 14.0%～20.0%, liver glycogen content improves 30.0%～37.0%, serum urea nitrogen has descended 25～30%, and the blood lactic acid content has reduced by 30～35%); Total production cost is relatively low, invests lessly, and products made thereby can directly use, and also can be used as food enrichment, improving protein content, and can apply to motion healthy food, military supplies food etc., has extensive use.
(4) specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment:
1. material liquid preliminary treatment
Selecting soybean isolate protein powder (protein quality degree 〉=80～83%) for use is raw material, adopts 90 ℃ water, makes percentage and be 5% enzymolysis substrate, after evenly stirring, 15min is handled in insulation, cool to 55 ℃ then, and regulator solution pH value is 8.5.
2. enzyme digestion reaction
Through adding mass percent 5%～20% protease in the pretreated material liquid enzymolysis substrate, under 55 ℃ condition, react 4h.
3. ultra-filtration and separation
After enzyme digestion reaction finishes, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da.Operating pressure is 0.2MPa, and temperature is 4 ℃.
4. spray-drying
Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 175 ℃ of inlet temperatures, 85 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa.
5. the sport polypeptide that adopts above protease hydrolyzed to make, product yield are 65.0%, and degree of hydrolysis is 18.0%.
Embodiment 1:
Present embodiment selects for use the efficient protein enzyme as enzyme preparation.Earlier with soy bean proteinous soln heat treated 15min in 90 ℃ of water-baths of 3%, cool to 55 ℃ then, regulator solution pH value is 8.0.Add 15% protease, react 4h under 55 ℃ of constant temperatures, recording the solution degree of hydrolysis is 16.3%.After enzyme digestion reaction finishes, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da.Operating pressure is 0.2MPa, and temperature is 4 ℃.Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 175 ℃ of inlet temperatures, 85 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa.
Embodiment 2:
Present embodiment selects for use the efficient protein enzyme as enzyme preparation.Earlier with soy bean proteinous soln heat treated 15min in 90 ℃ of water-baths of 5%, cool to 55 ℃ then, regulator solution pH value is 8.5.Add 15% protease, react 4h under 55 ℃ of constant temperatures, recording the solution degree of hydrolysis is 18.7%.After enzyme digestion reaction finishes, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da.Operating pressure is 0.2MPa, and temperature is 4 ℃.Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 175 ℃ of inlet temperatures, 85 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa.
Embodiment 3:
Present embodiment selects for use the efficient protein enzyme as enzyme preparation.Earlier with soy bean proteinous soln heat treated 15min in 90 ℃ of water-baths of 6%, cool to 55 ℃ then, regulator solution pH value is 8.5.Add 15% protease, react 4h under 55 ℃ of constant temperatures, recording the solution degree of hydrolysis is 17.3%.After enzyme digestion reaction finishes, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da.Operating pressure is 0.2MPa, and temperature is 4 ℃.Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 175 ℃ of inlet temperatures, 85 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa.
Embodiment 4:
Present embodiment selects for use the efficient protein enzyme as enzyme preparation.Earlier with soy bean proteinous soln heat treated 15min in 90 ℃ of water-baths of 4%, cool to 55 ℃ then, regulator solution pH value is 8.5.Add 20% protease, react 4h under 55 ℃ of constant temperatures, recording the solution degree of hydrolysis is 17.8%.After enzyme digestion reaction finishes, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da.Operating pressure is 0.2MPa, and temperature is 4 ℃.Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 175 ℃ of inlet temperatures, 85 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa.
Claims (1)
1, a kind of enzyme process is produced the method for soybean sport polypeptide, it is characterized in that described method comprises the steps:
(1) material liquid preliminary treatment
Selecting soybean isolate protein powder for use is raw material, soybean isolate protein powder protein content 〉=80～83%, adopt 75 ℃～90 ℃ water, make concentration and be 3%～6% enzymolysis substrate, after evenly stirring, 10～18min is handled in insulation, cool to 50 ℃～65 ℃ then, and regulator solution pH value is 7～9.5;
(2) enzyme digestion reaction
Adding 5%～20% protease, under 50 ℃～65 ℃ condition, react 2～5h through in the pretreated material liquid;
(3) ultra-filtration and separation
After enzyme digestion reaction finished, through the PS film ultrafilter ultrafiltration of dam molecular weight 1000Da, 5000Da, operating pressure was 0.1～0.5MPa, and temperature is 4 ℃～8 ℃;
(4) spray-drying
Each segment molecule amount polypeptide solution after separating is squeezed into spray drying tower through high-pressure pump carry out drying, drying condition is: 160 ℃～180 ℃ of inlet temperatures, 75 ℃～90 ℃ of outlet temperatures, high-pressure pump pressure 4.5MPa～5MPa;
(5) sport polypeptide that adopts protease hydrolyzed to make, the product yield is 60.0%～70.0%, degree of hydrolysis is 14.0%～20.0%.
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| CNA2009100712912A CN101485383A (en) | 2009-01-16 | 2009-01-16 | Method for preparing soybean sport polypeptide by enzyme method |
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| CNA2009100712912A CN101485383A (en) | 2009-01-16 | 2009-01-16 | Method for preparing soybean sport polypeptide by enzyme method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787387B (en) * | 2009-11-30 | 2012-08-15 | 广州合诚实业有限公司 | Acid-resistant no-bitterness soybean oligopeptide and production method and application thereof |
| CN102787014A (en) * | 2012-08-29 | 2012-11-21 | 东北农业大学 | Method for synchronously extracting oil and high-emulsification protein isolate from soybeans |
| CN106387920A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Preparation method of soybean polypeptide chelated calcium |
| CN106974108A (en) * | 2017-04-21 | 2017-07-25 | 安徽省领航动物保健品有限责任公司 | A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed |
-
2009
- 2009-01-16 CN CNA2009100712912A patent/CN101485383A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787387B (en) * | 2009-11-30 | 2012-08-15 | 广州合诚实业有限公司 | Acid-resistant no-bitterness soybean oligopeptide and production method and application thereof |
| CN102787014A (en) * | 2012-08-29 | 2012-11-21 | 东北农业大学 | Method for synchronously extracting oil and high-emulsification protein isolate from soybeans |
| CN102787014B (en) * | 2012-08-29 | 2013-06-12 | 东北农业大学 | Method for synchronously extracting oil and high-emulsification protein isolate from soybeans |
| CN106387920A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Preparation method of soybean polypeptide chelated calcium |
| CN106974108A (en) * | 2017-04-21 | 2017-07-25 | 安徽省领航动物保健品有限责任公司 | A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed |
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Open date: 20090722 |