CN106974108A

CN106974108A - A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed

Info

Publication number: CN106974108A
Application number: CN201710264948.1A
Authority: CN
Inventors: 臧春云; 唐传辉; 汪莉
Original assignee: Anhui Province Navigator's Animal Health-Care Product Co Ltd
Current assignee: Anhui Province Navigator's Animal Health-Care Product Co Ltd
Priority date: 2017-04-21
Filing date: 2017-04-21
Publication date: 2017-07-25

Abstract

The invention discloses the optimize technique that a kind of defatted soybean meal prepares small molecular protein peptide pig feed, ultramicro grinding after now defatted soybean meal is dried in vacuo, therefrom isolate again and use composite flavor enzyme to be thoroughly degraded to amino acid so as to a bitter peptide after soya-bean polypeptides, the bitter taste of peptide can be controlled, again can be to some picryls because being modified and being shifted, recombinated by the effect of Bacillus natto, during the fermentation, Soyprotein peptide is converted into small active peptides by metabolism, assimilation again between small peptide ammino acid.Cooperation with camellia oil simultaneously, can effectively improve its storge quality, reduce volatility, improve stability.Blood vessel elasticity and toughness can be strengthened with body simultaneously, increase stomach absorption function, promote endocrinal glands hormone secretion, improve immunity of organisms.

Description

A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed

Technical field

The excellent of small molecular protein peptide pig feed is prepared the present invention relates to field of feed, more particularly to a kind of defatted soybean meal Chemical industry skill.

Background technology

Soyprotein peptide is after soybean protein is acted on through acid system or enzyme hydrolysis, to be handled through the special process such as separation, refined Obtained from protein hydrolysate, be typically that the relative molecular mass that is made up of 3-6 amino acid is less than 1000 low peptide and mixed Compound.The amino acid composition of Soyprotein peptide is almost just the same with soybean protein, but digesting and assimilating in vivo is bright Aobvious to be better than free amino acid, it is not only nutritious, but also with adjustment blood sugar concentration, reduce blood pressure and suppress cholesterol, Bioactivity and the physiological functions such as anti-oxidant, antithrombotic, regulation immunologic function.《The biological function of active peptide of soybean protein and It is applied》Refer to that soya-bean polypeptides include some small-molecular peptides in one text, these small-molecular peptides are not hydrolyzed in animal gastrointestinal tract, no Directly absorbed by the interference of ANFs and, the infiltration rate than single amino acids is fast.These small-molecular peptides can be stimulated The growth of Ruminal Fibre decomposer and hormone function is played in animal body, therefore using small-molecular peptides as feed addictive just Arouse people's interest.

At present, the small-molecular peptides content in the Soyprotein peptide by extraction is limited, and is storing and using process In apt to deteriorate, corruption, instant invention overcomes above-mentioned problem, this experiment is by optimizing natto strains liquid fermentation soybean protein Technique, finds optimal fermentation condition, so as to improve the yield of low molecule Soyprotein peptide.This method production protein peptides be not by Soybean protein is simply cut into small peptide, but transposing also between small peptide, the process reset, can be right by microbial action Some picryls pass through metabolism because being modified and being shifted, recombinated, during the fermentation, between small peptide ammino acid, assimilated again, will Soyprotein peptide is converted into biologically active peptide.Cooperation with camellia oil simultaneously, can effectively improve its storge quality, reduction volatilization Property, improve stability.Blood vessel elasticity and toughness can be strengthened with body simultaneously, increase stomach absorption function, promote endocrinal glands Hormone secretion, improves immunity of organisms.

The content of the invention

The object of the invention is exactly to prepare small molecular protein peptide there is provided a kind of defatted soybean meal to make up the defect of prior art The optimize technique of pig feed.

The present invention is achieved by the following technical solutions：

A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed, and preparation method is comprised the following steps that：(1) will Defatted soybean meal first passes through coarse crushing after vacuum freeze drying, finely divided, then carries out ultramicro grinding, then Ultramicro-powder is pressed into 1:10 Solid-liquid ratio adds deionized water and is stirred, and regulation pH is 8.0-8.5, is stirred at room temperature 2-3 hours, then after centrifugation, separation Supernatant and precipitation, the pH of regulation supernatant is 4.5-4.8,10-13 points of centrifugation after standing 30-40 minutes under the conditions of 4-8 DEG C Clock, is precipitated；(2) precipitation obtained by step 2 is repeatedly rinsed after precipitation, with 1:10 solid-liquid ratio adds deionized water, adjusts Section pH is 7.5-7.8, and stirring makes it fully dissolve, and is dialysed 48-50 hours at 4-9 DEG C, obtains soybean protein isolate；(3) The soybean protein water content of regulating step 2 is 80-85%, is stirred with homogenizer, soybean protein solution is fully dissolved, While stirring to 90-95 DEG C and keeping constant temperature 5-6 minutes, 50-55 DEG C is cooled to, compound fertilizer production is added and is stirred continuously 10-12 hours, enzymolysis protein liquid is obtained, go out enzyme；(4) it is 8.0-8.3 by the protein liquid regulation pH after the enzyme that goes out, accesses Bacillus natto, 42-46 DEG C, shaken cultivation 10-12 hours under the conditions of 180r/min；(5) after the peptide liquid after step 4 is fermented is mixed with Camellia oil After homogenizer homogeneous, spray drying, you can.

Preferably, the condition of vacuum refrigeration is that temperature is subzero 25-20 DEG C in pomegranate seed in the step 1, and pressure is 15-20Pa。

Preferably, the step 3 adds the sodium carbonate liquor that mass fraction is 10-12% while stirring in enzymolysis process, PH is maintained in 5.6-5.9.

Preferably, the enzyme condition of going out of the step 3 is that enzymolysis liquid is heated into 80-85 DEG C, is kept for 5-8 minutes.

Preferably, the Bacillus natto inoculum concentration of the step 4 is 1.0-1.2%.

Preferably, the ratio of peptide liquid and Camellia oil is 5 in the step 5:1.

It is an advantage of the invention that：The compound fertilizer production of addition is when digesting peptide bond, due between carboxyl and amino Proton is exchanged, and the pH value of hydrolyzate can be made to decline automatically, influence enzymolysis speed, so it is molten constantly to add sodium carbonate Liquid, maintains suitable PH values.Single enzyme hydrolysis protein liquor will produce bitter taste under certain situation because of the bitter peptide of generation.The present invention is adopted Composite flavor enzyme is used, the bitter taste of peptide can be controlled, its principle is, by the peptide bond inside endo protease cut-out polypeptide, to be formed short Chain peptide, some of them contain hydrophobic amino acid, thus as bitter peptide, are released each time from the end cut-out of polypeptide chain using excision enzyme An amino acid is put, so that bitter peptide is thoroughly degraded to amino acid.It is big additionally by optimization natto strains liquid fermentation production low molecule The technique of legumin peptide, is improved the yield of tunning low molecule Soyprotein peptide.Peptide liquid is mixed with Camellia oil can To effectively improve its storge quality, volatility is reduced, stability is improved.Blood vessel elasticity and toughness can be strengthened with body simultaneously, increased Plus stomach absorption function, promote endocrinal glands hormone secretion, improve immunity of organisms.

Embodiment

A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed, and preparation method is comprised the following steps that： (1) defatted soybean meal is first passed through into coarse crushing after vacuum freeze drying, it is finely divided, then ultramicro grinding is carried out, then Ultramicro-powder is pressed 1:10 solid-liquid ratio adds deionized water and is stirred, and regulation pH is 8.0, is stirred at room temperature 2 hours, then after centrifugation, separation Supernatant and precipitation, the pH of regulation supernatant is 4.5, centrifuges 10 minutes, is precipitated after standing 30 minutes under the conditions of 4 DEG C； (2) precipitation obtained by step 2 is repeatedly rinsed after precipitation, with 1:10 solid-liquid ratio adds deionized water, and regulation pH is 7.5, Stirring makes it fully dissolve, and is dialysed 48 hours at 4 DEG C, obtains soybean protein isolate；(3) soybean protein of regulating step 2 contains Water is 80%, is stirred with homogenizer, soybean protein solution is fully dissolved, while stirring to 90 DEG C and keeping 5 points of constant temperature Clock, is cooled to 50 DEG C, adds compound fertilizer production and is stirred continuously 10 hours, obtains enzymolysis protein liquid, go out enzyme；(4) will go out enzyme Protein liquid regulation pH afterwards is 8.0, accesses Bacillus natto, shaken cultivation 10 hours under the conditions of 42 DEG C, 180r/min；(5) will step Peptide liquid after rapid 4 fermentation is used after homogenizer homogeneous after being mixed with Camellia oil, spray drying, you can.

Preferably, the condition of vacuum refrigeration is that temperature is subzero 25 DEG C in pomegranate seed in the step 1, and pressure is 20Pa.

Preferably, the step 3 adds the sodium carbonate liquor that mass fraction is 10-12% while stirring in enzymolysis process, PH is maintained 5.6.

Preferably, the enzyme condition of going out of the step 3 is that enzymolysis liquid is heated into 80 DEG C, is kept for 5 minutes.

Preferably, the Bacillus natto inoculum concentration of the step 4 is 1.0%.

Preferably, the ratio of peptide liquid and Camellia oil is 5 in the step 5:1.

Claims

1. a kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed, it is characterised in that preparation method it is specific Step is as follows：(1) defatted soybean meal is first passed through into coarse crushing after vacuum freeze drying, it is finely divided, then ultramicro grinding is carried out, then will Ultramicro-powder presses 1:10 solid-liquid ratio adds deionized water and is stirred, and regulation pH is 8.0-8.5, is stirred at room temperature 2-3 hours, then After centrifugation, separation supernatant and precipitation, the pH of regulation supernatant is 4.5-4.8, and 30-40 minutes are stood under the conditions of 4-8 DEG C Centrifuge 10-13 minutes, precipitated afterwards；(2) precipitation obtained by step 2 is repeatedly rinsed after precipitation, with 1:10 solid-liquid ratio adds Enter deionized water, regulation pH is 7.5-7.8, and stirring makes it fully dissolve, and is dialysed 48-50 hours at 4-9 DEG C, obtains soybean Protein isolate；(3) the soybean protein water content of regulating step 2 is 80-85%, is stirred with homogenizer, makes soybean protein Liquid fully dissolves, while stirring to 90-95 DEG C and keeping constant temperature 5-6 minutes, is cooled to 50-55 DEG C, adds compound fertilizer production And be stirred continuously 10-12 hours, enzymolysis protein liquid is obtained, go out enzyme；(4) it is 8.0-8.3 by the protein liquid regulation pH after the enzyme that goes out, connects Enter Bacillus natto, shaken cultivation 10-12 hours under the conditions of 42-46 DEG C, 180r/min；(5) peptide liquid and tea after step 4 is fermented Used after caul-fat mixing after homogenizer homogeneous, spray drying, you can.

2. the method according to claims 1, it is characterised in that in the step 1 in pomegranate seed vacuum refrigeration condition It is that temperature is subzero 25-20 DEG C, pressure is 15-20Pa.

3. the method according to claims 1, it is characterised in that：The step 3 is added while stirring in enzymolysis process Mass fraction is 10-12% sodium carbonate liquor, maintains PH in 5.6-5.9.

4. the method according to claims 1, it is characterised in that：The enzyme condition of going out of the step 3 is to heat enzymolysis liquid To 80-85 DEG C, kept for 5-8 minutes.

5. the method according to claims 1, it is characterised in that：The Bacillus natto inoculum concentration of the step 4 is 1.0-1.2%.

6. the method according to claims 1, it is characterised in that：The ratio of peptide liquid and Camellia oil is 5 in the step 5: 1。