CN1765921A - Separated soybean protein preparation process and prepared soybean small peptide and process - Google Patents
Separated soybean protein preparation process and prepared soybean small peptide and process Download PDFInfo
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- CN1765921A CN1765921A CN 200510126539 CN200510126539A CN1765921A CN 1765921 A CN1765921 A CN 1765921A CN 200510126539 CN200510126539 CN 200510126539 CN 200510126539 A CN200510126539 A CN 200510126539A CN 1765921 A CN1765921 A CN 1765921A
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Abstract
The invention discloses a new synthesis method for bicetyl pentaerythritol diphosphite ester, which comprises two steps shown below: 1. synthesis to pentaerythritol diphosphite ester; 2. synethsis to the product. This invention needs no organic solvent and generates no phenol, so environmental friendly.
Description
Technical field
The present invention relates to soybean deep processing field, particularly a kind of method and Semen sojae atricolor small peptide product of producing soybean protein isolate and Semen sojae atricolor small peptide.
Background technology
Soybean protein isolate is rich in eight kinds of indispensable amino acids and other non-essential amino acid such as Methionin, tryptophane, Xie Ansuan, and composition of amino acid meets The World Health Organization (WHO) and requires edible protein amino acid ratio, is defined as the high-quality protein source by WHO.Soybean protein isolate has characteristics such as water-retentivity, tackiness, gelation, oil absorbency and emulsifying property, be widely used as the foodstuffs industry basic material, also can directly enter the consumption market, for the human consumer provides quality plant protein, can also be used widely in fields such as feed processing, pharmacy, plastics industries.The little peptide of soybean protein is the soy-protein segment of molecular weight below 1000D of forming with 2-8 amino acid.Studies show that Semen sojae atricolor small peptide is higher with free canclin nutritive value than soybean protein isolate: (1) is compared with the running total free aminoacids, and body running Semen sojae atricolor small peptide is faster by the speed of intestines wall; (2) the osmotic pressure specific ionization amino acid of Semen sojae atricolor small peptide is low, therefore can improve the specific absorption of Semen sojae atricolor small peptide; (3) because the molecular weight of Semen sojae atricolor small peptide is little, antigenicity is lower than macromole peptide and prototype protein antigenicity; (4) Semen sojae atricolor small peptide has many special physiological functions such as hypotensive, reducing blood-fat, hypoglycemic etc.; (5) Semen sojae atricolor small peptide solvability, processibility is good, and better solvability is all arranged in food-processing temperature and concentration range; (6) Semen sojae atricolor small peptide has good sensation and sense of taste effect.
More about the document and the patent of producing soybean protein isolate and Semen sojae atricolor small peptide method at present, but all be to transfer pH value with 10-20% hydrochlorinate sodium liquid.Because sodium hydroxide is highly basic, transfer pH value to have following shortcoming with it: 1. the bean cake powder grain expansion is too much, and part causes influences extract yield by peptization. general yield is all at (with respect to the dregs of beans raw material) below 45%; Filtration velocity when 2. influence separates; Washing difficulty when 3. increasing bean dregs as food fibre; Degree of hydrolysis when 4. influencing the Semen sojae atricolor small peptide enzymolysis and yield.Also have enzymolysis process to produce in the prior art of Semen sojae atricolor small peptide, single enzyme or the multienzyme mixed enzymolysis of adopting more, the percent hydrolysis of single enzymolysis is low, and used multi-enzymatic hydrolysis all adopts the mixed enzyme of autogamy or plurality of enzymes to add respectively now, such method had both increased production cost, made complex production process again.
Summary of the invention
The purpose of this invention is to provide a kind of method of producing soybean protein isolate and Semen sojae atricolor small peptide, for solving the above-mentioned problems in the prior art, adopt solution of ammonium hydroxide regulator solution pH value in process of production, also adopt the compound protease enzymolysis of now selling to produce the little peptide of soybean protein, the yield and the quality product of soybean protein isolate extraction yield, Semen sojae atricolor small peptide are improved, also reduce this two kinds of products production costs, strengthened this two kinds of competitiveness of product in market.
Technical solution of the present invention is:
A kind of preparation technology of soybean protein isolate, raw material is an edible bean cake, step is:
A, edible bean cake are pulverized and are added 6-10 water doubly, regulate PH8.0-10.5 with the solution of ammonium hydroxide of 15%-20%, extract 0.5-2h, separation and Extraction liquid and slag at 40 ℃-65 ℃;
B, slag repeat to extract once by the 6-10 water doubly of a step with the dregs of beans amount, separate extracting solution, merge secondary raffinate;
C, extracting solution precipitate with acid accent PH4.0-5.0, the soybean protein isolate that separates wetly, the dehydration of washing back, the dry soybean protein isolate that gets.
A kind of Semen sojae atricolor small peptide is made of soybean protein isolate, and molecular weight accounts for more than total protein 90% or 90% less than the protein component of 1000D.
A kind of preparation technology of Semen sojae atricolor small peptide, raw material is an edible bean cake, step is:
A, edible bean cake are pulverized and are added 6-10 water doubly, regulate PH8.0-10.5 with the solution of ammonium hydroxide of 15%-20%, soak separation and Extraction liquid and slag;
B, slag repeat to extract once by the 6-10 water doubly of a step with the dregs of beans amount, separate extracting solution, merge secondary raffinate;
C, extracting solution are transferred PH4.0-5.0 with acid, precipitation, the soybean protein isolate that separates wetly;
D, will be wet the soybean protein isolate washing after add the 6-10 water-dispersion doubly of dregs of beans amount, regulate PH8-10 with the ammonium hydroxide of 15%-20%, add the compound protease enzymolysis, filter enzymolysis solution;
E, enzymolysis solution concentrate, the dry Semen sojae atricolor small peptide that gets.
A kind of preparation technology of Semen sojae atricolor small peptide, raw material is the soybean protein isolate of protein content more than 85%, step is:
A, with adding 6-15 water-dispersion doubly after the wet soybean protein isolate washing, regulate PH8-10 with ammonium hydroxide, add the compound protease enzymolysis, filter enzymolysis solution;
B, enzymolysis solution concentrate, the dry Semen sojae atricolor small peptide that gets.
Described edible bean cake is pulverized and is the 30-60 order.
Described acid is hydrochloric acid or citric acid, concentration 15-20%.
Described wet soybean protein isolate disperses washing with deionized water, is deployed into the muddy liquid of solid content 15-30%, homogeneous, and spraying drying gets soybean protein isolate.
Described compound protease is the compound protease 3111 that dull biotechnology research centre, Yinchuan produces, during enzymolysis, and concentration of substrate 5-8%, with enzyme amount 0.1-1.0% (W/V), hydrolysis temperature 40-50 ℃, enzymolysis time 3-5h, the enzyme after-filtration goes out.
Being concentrated in economic benefits and social benefits or the triple effect thickner of described enzymolysis solution carried out, and is concentrated into solid content 30-50%, and drying is carried out in centrifugal or press spray drying tower.
According to the present invention, extract soybean protein isolate and yield and bring up to more than 45%, the yield for preparing Semen sojae atricolor small peptide thus is near 40%, far above the yield of prior art.The present invention adopts and now sells the finished product compound protease, and enzymolysis time shortens to 3-5 hour by 7-12 hour of prior art, and during enzymolysis, compound protease acts on the different loci that amino acid connects in the protein simultaneously, shorten enzymolysis time, improve enzymolysis efficiency, reduced cost significantly.According to the Semen sojae atricolor small peptide that the present invention produces, protein molecular weight directly is not an absorption of human body by Digestive tract less than 1000D, absorbs fast, and is nutritious.
Embodiment
Embodiment one
Edible bean cake 100Kg, pulverizing is 30 orders, the water that adds 600 kilograms stirs evenly, and regulates PH8.0 with 15% solution of ammonium hydroxide, extracts 2h at 40 ℃, precipitation separation, extracting solution and slag.Slag adds 600 kilograms water, regulates PH8.0 with 15% solution of ammonium hydroxide, extracts 2h at 40 ℃, separate extracting solution, merge secondary raffinate.Extracting solution is with 15% hydrochloric acid, transfer PH4.0, stir evenly and leave standstill, precipitation separation, the soybean protein isolate that must wet, wet soybean protein isolate deionized water wash, be deployed into the muddy liquid of solid content 15%, homogeneous, spraying drying gets soybean protein isolate 49.8Kg, protein content 〉=90%, yield are 49.8%.
Embodiment two
Edible bean cake 100Kg, pulverizing is 50 orders, the water that adds 800 kilograms stirs evenly, and regulates PH9 with 18% solution of ammonium hydroxide, extracts 1h at 50 ℃, precipitation separation, extracting solution and slag.Slag adds 800 kilograms water again, regulates PH9 with 18% solution of ammonium hydroxide, extracts 1h at 50 ℃, separate extracting solution, merge secondary raffinate.Extracting solution is with 18% citric acid, transfer PH4.5, stir evenly and leave standstill, precipitation separation, the soybean protein isolate that must wet, wet soybean protein isolate disperses washing with deionized water, be deployed into the muddy liquid of solid content 23%, homogeneous, spraying drying gets soybean protein isolate 49.5Kg, protein content 〉=90%, yield are 49.5%.
Embodiment three
Edible bean cake 100Kg, pulverizing is 60 orders, the water that adds 1000 kilograms stirs evenly, and regulates PH10.5 with 20% solution of ammonium hydroxide, extracts 2h at 65 ℃, precipitation separation, extracting solution and slag.Regulate PH10.5 with 20% solution of ammonium hydroxide, extract 2h at 65 ℃, separate extracting solution, merge secondary raffinate; Extracting solution is with 20% hydrochloric acid or citric acid, transfer PH5.0, precipitation, the soybean protein isolate that separates wetly, wet soybean protein isolate disperses washing with deionized water, is deployed into solid content 30% muddy liquid, homogeneous, spraying drying gets soybean protein isolate 50.1Kg, and protein content 〉=90%, yield are 50.1%.
Embodiment four
According to embodiment one, behind the soybean protein isolate that second extraction must wet, with adding 600 kilograms water-dispersion after the wet soybean protein isolate washing, regulate PH8 with 15% solution of ammonium hydroxide, add compound protease 3111 enzymolysis that dull biotechnology research centre, Yinchuan produces.During enzymolysis, concentration of substrate 5%, (adopt weightmeasurement ratio to calculate, the volume when establishing enzymolysis herein is 100V with 0.1 kilogram of enzyme amount, following examples are herewith estimated), 40 ℃ of hydrolysis temperatures, enzymolysis time 3h, enzymolysis are finished and are warming up to 85 ℃ of enzymes 10 minutes of going out, be cooled to 70 ℃, add 1 kilogram of 303 sugar charcoal, stir decolouring 30 minutes, with hyperfiltration process filter enzymolysis solution.Enzymolysis solution is concentrated into solid content 30% in the economic benefits and social benefits thickner, dry in centrifugal spray-drying tower.
After finishing, above-mentioned drying step makes 38.5 kilograms in finished product Semen sojae atricolor small peptide powder, yield 38.5%, and protein content 90%, molecular weight is 90% less than the protein component of 1000D.
Protein content 〉=90%.
Embodiment five
According to embodiment two, behind the soybean protein isolate that second extraction must wet, with adding 800 kilograms water-dispersion after the wet soybean protein isolate washing, regulate PH9 with 18% solution of ammonium hydroxide, add compound protease 3111 enzymolysis that dull biotechnology research centre, Yinchuan produces.During enzymolysis, concentration of substrate 7%, with 0.5 kilogram of enzyme amount, 45 ℃ of hydrolysis temperatures, enzymolysis time 4h, enzymolysis finish and are warming up to 90 ℃ of enzymes 5 minutes of going out, and are cooled to 75 ℃, add 1.5 kilograms of 303 sugar charcoals, stirring decolouring 40 minutes is with getting enzymolysis solution after whizzer centrifugal filtration and the smart filter of microporous membrane.Enzymolysis solution is concentrated into solid content 40% in the triple effect thickner, dry in the press spray drying tower.
After finishing, above-mentioned drying step makes 39.7 kilograms in finished product Semen sojae atricolor small peptide powder, yield 39.7%, and protein content 95%, molecular weight is 95% less than the protein component of 1000D.
Embodiment six
According to embodiment three, behind the soybean protein isolate that second extraction must wet, with adding 1000 kilograms water-dispersion after the wet soybean protein isolate washing, regulate PH10 with 20% solution of ammonium hydroxide, add compound protease 3111 enzymolysis that dull biotechnology research centre, Yinchuan produces.During enzymolysis, concentration of substrate 8%, with 1 kilogram of enzyme amount, 50 ℃ of hydrolysis temperatures, enzymolysis time 5h, enzymolysis finish and are warming up to 95 ℃ of enzymes 3 minutes of going out, and are cooled to 80 ℃, add 2 kilograms of 303 sugar charcoals, stir decolouring 10 minutes, get enzymolysis solution with plate-and-frame filter press and ceramic capillary membrane filtration.Enzymolysis solution is concentrated into solid content 50% in the economic benefits and social benefits thickner, dry in the press spray drying tower.
Make finished product after above-mentioned drying step is finished and get 39.2 kilograms in Semen sojae atricolor small peptide powder, yield 39.2%, protein content 99%, molecular weight is 99% less than the protein component of 1000D.
Embodiment seven
Get the soybean protein isolate 49.8Kg that makes by embodiment one, add 600 kilograms water-dispersion, regulate PH8, add compound protease 3111 enzymolysis that dull biotechnology research centre, Yinchuan produces with 15% solution of ammonium hydroxide.During enzymolysis, concentration of substrate 5%, with 0.1 kilogram of enzyme amount, 40 ℃ of hydrolysis temperatures, enzymolysis time 3h, enzymolysis are finished and are warming up to 85 ℃ of enzymes 10 minutes of going out, and are cooled to 70 ℃, add 1 kilogram of 303 sugar charcoal, stir decolouring 30 minutes, with hyperfiltration process filter enzymolysis solution.Enzymolysis solution is concentrated into solid content 30% in the economic benefits and social benefits thickner, dry in centrifugal spray-drying tower.Make 38.5 kilograms in finished product Semen sojae atricolor small peptide powder after the drying, yield 77.3%, protein content 90%, molecular weight is 90% less than the protein component of 1000D.
Embodiment eight
Get the soybean protein isolate 49.5Kg that makes by embodiment two, add 1000 kilograms water-dispersion, regulate PH9, add compound protease 3111 enzymolysis that dull biotechnology research centre, Yinchuan produces with 18% solution of ammonium hydroxide.During enzymolysis, concentration of substrate 7%, with 0.5 kilogram of enzyme amount, 45 ℃ of hydrolysis temperatures, enzymolysis time 4h, enzymolysis finish and are warming up to 90 ℃ of enzymes 5 minutes of going out, and are cooled to 75 ℃, add 1.5 kilograms of 303 sugar charcoals, stirring decolouring 40 minutes is with getting enzymolysis solution after whizzer centrifugal filtration and the smart filter of microporous membrane.Enzymolysis solution is concentrated into solid content 40% in the triple effect thickner, dry in the press spray drying tower.Make 39.7 kilograms in finished product Semen sojae atricolor small peptide powder after the drying, yield 80.2%, protein content 95%, molecular weight is 95% less than the protein component of 1000D.
Embodiment six
Get the soybean protein isolate 50.1Kg that makes by embodiment three, add 1500 kilograms water-dispersion, regulate PH10, add compound protease 3111 enzymolysis that dull biotechnology research centre, Yinchuan produces with 20% solution of ammonium hydroxide.During enzymolysis, concentration of substrate 8%, with 1 kilogram of enzyme amount, 50 ℃ of hydrolysis temperatures, enzymolysis time 5h, enzymolysis finish and are warming up to 95 ℃ of enzymes 3 minutes of going out, and are cooled to 80 ℃, add 2 kilograms of 303 sugar charcoals, stir decolouring 10 minutes, get enzymolysis solution with plate-and-frame filter press and ceramic capillary membrane filtration.Enzymolysis solution is concentrated into solid content 50% in the economic benefits and social benefits thickner, dry in the press spray drying tower.Make finished product after the drying and get 39.2 kilograms in Semen sojae atricolor small peptide powder, yield 78.2%, protein content 99%, molecular weight is 99% less than the protein component of 1000D.
Claims (11)
1, a kind of preparation technology of soybean protein isolate, raw material is an edible bean cake, step is:
A, edible bean cake are pulverized and are added 6-10 water doubly, regulate PH8.0-10.5 with the solution of ammonium hydroxide of 15%-20%, extract 0.5-2h, separation and Extraction liquid and slag at 40 ℃-65 ℃;
B, slag repeat to extract once by the 6-10 water doubly of a step with the dregs of beans amount, separate extracting solution, merge secondary raffinate;
C, extracting solution precipitate with acid accent PH4.0-5.0, the soybean protein isolate that separates wetly, the dehydration of washing back, the dry soybean protein isolate that gets.
2, a kind of Semen sojae atricolor small peptide is made of soybean protein isolate, it is characterized in that molecular weight accounts for more than total protein 90% or 90% less than the protein component of 1000D.
3, the preparation technology of Semen sojae atricolor small peptide as claimed in claim 2, raw material is an edible bean cake, step is:
A, edible bean cake are pulverized and are added 6-10 water doubly, regulate PH8.0-10.5 with the solution of ammonium hydroxide of 15%-20%, soak separation and Extraction liquid and slag;
B, slag repeat to extract once by the 6-10 water doubly of a step with the dregs of beans amount, separate extracting solution, merge secondary raffinate;
C, extracting solution are transferred PH4.0-5.0 with acid, precipitation, the soybean protein isolate that separates wetly;
D, will be wet the soybean protein isolate washing after add the 6-10 water-dispersion doubly of dregs of beans amount, regulate PH8-10 with the ammonium hydroxide of 15%-20%, add the compound protease enzymolysis, filter enzymolysis solution;
E, enzymolysis solution concentrate, the dry Semen sojae atricolor small peptide that gets.
4, the preparation technology of Semen sojae atricolor small peptide as claimed in claim 2, raw material is the soybean protein isolate of protein content more than 85%, step is:
A, with adding 6-15 water-dispersion doubly after the wet soybean protein isolate washing, regulate PH8-10 with ammonium hydroxide, add the compound protease enzymolysis, filter enzymolysis solution;
B, enzymolysis solution concentrate, the dry Semen sojae atricolor small peptide that gets.
5, the preparation technology of soybean protein isolate as claimed in claim 1 is characterized in that it is the 30-60 order that step a edible bean cake is pulverized.
6, the preparation technology of Semen sojae atricolor small peptide as claimed in claim 3 is characterized in that it is the 30-60 order that step a edible bean cake is pulverized.
7, the preparation technology of soybean protein isolate as claimed in claim 1 is characterized in that the acid in the c step is hydrochloric acid or citric acid, concentration 15-20%.
8, the preparation technology of Semen sojae atricolor small peptide as claimed in claim 3 is characterized in that the acid in the c step is hydrochloric acid or citric acid, concentration 15-20%.
9, the preparation technology of soybean protein isolate as claimed in claim 1 is characterized in that the soybean protein isolate that wets disperses washing with deionized water, is deployed into the muddy liquid of solid content 15-30%, homogeneous, and spraying drying gets soybean protein isolate.
10, as the preparation technology of claim 3 or 4 described Semen sojae atricolor small peptide, it is characterized in that compound protease is the compound protease 3111 that dull biotechnology research centre, Yinchuan produces, during enzymolysis, concentration of substrate 5-8%, with enzyme amount 0.1-1.0% (W/V), hydrolysis temperature 40-50 ℃, enzymolysis time 3-5h, the enzyme after-filtration goes out.
11, as the preparation technology of claim 3 or 4 described Semen sojae atricolor small peptide, it is characterized in that being concentrated in economic benefits and social benefits or the triple effect thickner of enzymolysis solution carry out, be concentrated into solid content 30-50%, dryly in centrifugal or press spray drying tower, carry out.
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| CN 200510126539 CN1765921A (en) | 2005-11-28 | 2005-11-28 | Separated soybean protein preparation process and prepared soybean small peptide and process |
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| CN 200510126539 CN1765921A (en) | 2005-11-28 | 2005-11-28 | Separated soybean protein preparation process and prepared soybean small peptide and process |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101988080A (en) * | 2009-08-01 | 2011-03-23 | 山东御馨豆业蛋白有限公司 | Method for producing soybean small peptide |
| CN102018092A (en) * | 2010-12-21 | 2011-04-20 | 山东省鲁洲食品集团有限公司 | Method for extracting protein from maize germ dregs |
| CN104397678A (en) * | 2014-11-28 | 2015-03-11 | 哈尔滨墨医生物技术有限公司 | Protein powder preparation and production method thereof |
| CN106974108A (en) * | 2017-04-21 | 2017-07-25 | 安徽省领航动物保健品有限责任公司 | A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed |
| CN107252117A (en) * | 2017-06-15 | 2017-10-17 | 大连豪翔生物酶工程有限公司 | A kind of method that oligopeptide, polysaccharide and dietary fiber are produced by raw material of peanut meal |
| CN108391747A (en) * | 2018-02-06 | 2018-08-14 | 林坚 | A kind of preparation method of small molecular soybean protein peptide |
| CN109170124A (en) * | 2018-09-25 | 2019-01-11 | 山东禹王生态食业有限公司 | The method of ultrasonic wave added alkalinity extraction soybean protein isolate |
| CN109527196A (en) * | 2018-12-17 | 2019-03-29 | 沈阳市康普利德生物科技有限公司 | A method of improving hydrolysate of soybean protein efficiency and yield |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN110938121A (en) * | 2019-12-10 | 2020-03-31 | 东北农业大学 | Preparation method of alcohol-soluble soybean protein |
-
2005
- 2005-11-28 CN CN 200510126539 patent/CN1765921A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101988080A (en) * | 2009-08-01 | 2011-03-23 | 山东御馨豆业蛋白有限公司 | Method for producing soybean small peptide |
| CN102018092A (en) * | 2010-12-21 | 2011-04-20 | 山东省鲁洲食品集团有限公司 | Method for extracting protein from maize germ dregs |
| CN104397678A (en) * | 2014-11-28 | 2015-03-11 | 哈尔滨墨医生物技术有限公司 | Protein powder preparation and production method thereof |
| CN106974108A (en) * | 2017-04-21 | 2017-07-25 | 安徽省领航动物保健品有限责任公司 | A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed |
| CN107252117A (en) * | 2017-06-15 | 2017-10-17 | 大连豪翔生物酶工程有限公司 | A kind of method that oligopeptide, polysaccharide and dietary fiber are produced by raw material of peanut meal |
| CN108391747A (en) * | 2018-02-06 | 2018-08-14 | 林坚 | A kind of preparation method of small molecular soybean protein peptide |
| CN109170124A (en) * | 2018-09-25 | 2019-01-11 | 山东禹王生态食业有限公司 | The method of ultrasonic wave added alkalinity extraction soybean protein isolate |
| CN109527196A (en) * | 2018-12-17 | 2019-03-29 | 沈阳市康普利德生物科技有限公司 | A method of improving hydrolysate of soybean protein efficiency and yield |
| CN109527196B (en) * | 2018-12-17 | 2022-02-18 | 辽宁康普利德生物科技有限公司 | Method for improving enzymolysis efficiency and yield of soybean protein |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN110938121A (en) * | 2019-12-10 | 2020-03-31 | 东北农业大学 | Preparation method of alcohol-soluble soybean protein |
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