CN104904988A - Method for processing peanut protein powder with high polypeptide content - Google Patents
Method for processing peanut protein powder with high polypeptide content Download PDFInfo
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- CN104904988A CN104904988A CN201510335506.2A CN201510335506A CN104904988A CN 104904988 A CN104904988 A CN 104904988A CN 201510335506 A CN201510335506 A CN 201510335506A CN 104904988 A CN104904988 A CN 104904988A
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Abstract
The invention belongs to the technical field of food processing and particularly relates to a method for processing peanut protein powder with high polypeptide content. The method comprises the following steps: crushing peanut meal, then degreasing the crushed peanut meal by utilizing a degreasing agent formed by cyclohexane, ethyl acetate and triethanolamine, extruding, puffing, crushing, carrying out primary high-pressure homogenization, and then carrying out enzymolysis by utilizing alkaline protease; after enzymolysis, adding papain for debitterizing enzymatic hydrolysate; and carrying out secondary high-pressure homogenization, spraying and drying. According to the processing method, the efficiency is high, the polypeptide content of the prepared peanut protein powder is high, the flour yield is high, and the stability, water holding capability and foaming performance of the prepared peanut protein powder are good.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to a kind of processing method of high content of peptides peanut protein powder.
Background technology
Peanut is the important oil crops of China and industrial crops, produces 7830000 tons per year, occupies first place in the world.Peanut is also a kind of well plant protein resource, and in peanut, protein content is 26%-33%, is only second to soybean and occupies second in oil crops.The peanut that China produces is except small part is edible as dry fruit, and major part is for squeezing edible oil and fat as oil resource.After oil expression, gained accounts for protein content in the peanut dregs of peanut gross weight 56%-61% is about 55%, and its amino acid composition is comparatively complete, and containing a large amount of essential amino acid, the model proteins matter that nutritive value can be recommended with FAO compares favourably.Peanut dregs is used as feed or fertilizer more, and not yet fully deep processing and utilization, thus causes the significant wastage of peanut protein resource.
Peanut Polypeptide refers to that peanut protein is after protease effect, then through the protein hydrolysate that process obtains such as being separated, refining.Its total nitrogen all reaches more than 11.3%, and molecular weight is below 26600, water-soluble all more than 90%.The biologically actives such as Peanut Polypeptide is anti-oxidant, hypotensive, also have the functional characteristic of high-dissolvability, high-hygroscopicity, few allergy etc.Peanut protein aboundresources, cheap, be the good plant protein resource of enzyme-squash techniqued biologically active peptide.In the processing method of existing peanut protein powder, degreasing time is long, and the peanut protein powder content of peptides of preparation is lower, and the flour extraction after spraying dry is low.
Summary of the invention
For prior art Problems existing, the invention provides a kind of processing method of high content of peptides peanut protein powder, this processing method efficiency is high, and the peanut protein powder content of peptides of preparation is high, and flour extraction is high.
The technical scheme that the present invention is adopted to achieve these goals is:
The invention provides a kind of processing method of high content of peptides peanut protein powder, comprise the following steps:
(1) degreasing
Peanut dregs is crushed to 70 orders, adds the degreasing solvent be made up of cyclohexane, ethyl acetate and triethanolamine, stirs 30-40min, vacuum filtration under 25-30 DEG C of condition, cleaning, and namely aeration-drying obtain defatted peanut protein powder;
(2) extrusion is pulverized
It is 30-40% that defatted peanut protein powder adds water to water content, the expanded 1-3min of twin-screw extruder, and outlet temperature is 100-120 DEG C, dry under 50-60 DEG C of condition, is then crushed to 80-100 order;
(3) once high-pressure homogeneous
The peanut protein powder pulverized adds the water of 3-4 times of weight, stirs fully and carries out homogeneous by high pressure homogenizer, form uniform peanut protein emulsion;
(4) enzymolysis polypeptide
Adding alkali protease by peanut protein emulsion, is 8.0 at pH value, and temperature is enzymolysis 5h under 55 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 5-10min that goes out, obtains polypeptide peanut protein emulsion after enzymolysis terminates;
(5) polypeptide debitterize
Adding papain by polypeptide peanut protein emulsion, is 7.0 at pH value, and temperature is debittering by enzymatic hydrolysis 5h under 70 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 5-10 min that goes out after enzymolysis terminates:
(6) secondary high-pressure homogeneous
Carry out homogeneous by high pressure homogenizer after debitterize terminates, form uniform peanut protein concentrate;
(7) spraying dry
Peanut protein concentrate is added whey isolate protein, then carries out spraying dry, obtain high content of peptides peanut protein powder.
Further, in step (1), described degreasing solvent is 2-3:1 with the mass ratio of peanut dregs.
Above-mentioned degreasing solvent is made up of according to mass ratio 2:1:0.3 cyclohexane, ethyl acetate and triethanolamine.
Further, described once high-pressure homogeneous and processing condition that is secondary high-pressure homogeneous is: pressure 20-25MPa, temperature 60-70 DEG C.
Further, the addition of described alkali protease accounts for 2% of peanut protein emulsion weight.
Further, the addition of described papain accounts for 1.5% of polypeptide peanut protein emulsion weight.
Further, the addition of described whey isolate protein accounts for 1% of peanut protein concentrate weight.
Further, described spray-dired condition is: stream pressure is 0.25 M Pa, sample rate 7.2 mL/s, EAT 190 DEG C, leaving air temp 80 DEG C.
The present invention adds a small amount of triethanolamine in traditional degreasing solvent cyclohexane, ethyl acetate, triethanolamine with the residual aliphatic acid neutralization in peanut dregs, can improve degreasing efficiency, simultaneously, triethanolamine can metal ion in complexing peanut dregs, protects look to peanut protein powder.Inventor is found by lot of experiments, after structure by extrusion and high-pressure homogeneous loose peanut protein, the quantity of restriction enzyme site can be increased, thus improve the yield of polypeptide, well known to a person skilled in the art it is that enzyme has specificity, adopt different enzymes and enzymolysis time to the local flavor of product and substance classes all different with content, by addition and the addition sequence of different enzymes, after enzymolysis, obtain the high content of peptides peanut protein powder of raciness.The present invention is at spray-drying stage, add a small amount of whey isolate protein, one deck small protein molecular film can be formed rapidly at large protein molecular surface, improve the flour extraction of peanut protein powder, reduce the generation of wall sticking phenomenon, strengthen the nutritive value of products obtained therefrom, molecular film can be good at blocking oxygen simultaneously, therefore, the storage time of peanut protein powder is extended.
Beneficial effect of the present invention is:
(1) add a small amount of triethanolamine in skimming processes, degreasing efficiency can be improved, reduce the residual quantity of organic solvent in material.
(2) by extrusion and homogeneous process, enzymolysis efficiency is high, and improve content of peptides, stability, retentiveness and frothing capacity are good.
(3) by adding a small amount of whey isolate protein, improving the nutritive value that flour extraction strengthens peanut protein powder simultaneously, extending the storage time.
Detailed description of the invention
For a better understanding of the present invention, be described further below by specific embodiment.
The alkali protease used in the present invention, papain, purchased from Dong Henghua road, Nanning biotechnology Co., Ltd.
Embodiment 1
A processing method for high content of peptides peanut protein powder, comprises the following steps:
(1) degreasing
Peanut dregs is crushed to 70 orders, adds the degreasing solvent be made up of according to mass ratio 2:1:0.3 cyclohexane, ethyl acetate and triethanolamine, and the mass ratio of degreasing solvent and peanut dregs is 2:1,30min is stirred under 30 DEG C of conditions, vacuum filtration, cleaning, namely aeration-drying obtain defatted peanut protein powder;
(2) extrusion is pulverized
It is 30% that defatted peanut protein powder adds water to water content, the expanded 1min of twin-screw extruder, and outlet temperature is 100 DEG C, dry under 50 DEG C of conditions, is then crushed to 100 orders.
(3) once high-pressure homogeneous
The peanut protein powder pulverized adds the water of 3 times of weight, stirs fully and carries out homogeneous 6 times by high pressure homogenizer at pressure 20MPa, temperature 70 C, form uniform peanut protein emulsion.
(4) enzymolysis polypeptide
To add the alkali protease accounting for its weight 2% in peanut protein emulsion, is 8.0 at pH value, and temperature is enzymolysis 5h under 55 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 5min that goes out, obtains polypeptide peanut protein emulsion after enzymolysis terminates;
(5) polypeptide debitterize
Adding in polypeptide peanut protein emulsion the papain accounting for its weight 1.5%, is 7.0 at pH value, and temperature is debittering by enzymatic hydrolysis 5h under 70 DEG C of conditions, is warming up to 100-105 DEG C of enzyme 10 min that goes out after enzymolysis terminates:
(6) secondary high-pressure homogeneous
Under pressure 25MPa, temperature 60 C condition, carry out second homogenate 4 times by high pressure homogenizer after debitterize terminates, form uniform peanut protein concentrate.
(7) spraying dry
Peanut protein concentrate is added the whey isolate protein accounting for its weight 1%, then carry out spraying dry, condition is: stream pressure is 0.25 M Pa, sample rate 7.2 mL/s, EAT 190 DEG C, leaving air temp 80 DEG C, obtains high content of peptides peanut protein powder.
Embodiment 2
A processing method for high content of peptides peanut protein powder, comprises the following steps:
(1) degreasing
Peanut dregs is crushed to 70 orders, adds the degreasing solvent be made up of according to mass ratio 2:1:0.3 cyclohexane, ethyl acetate and triethanolamine, and the mass ratio of degreasing solvent and peanut dregs is 3:1,40min is stirred under 25 DEG C of conditions, vacuum filtration, cleaning, namely aeration-drying obtain defatted peanut protein powder;
(2) extrusion is pulverized
It is 36% that defatted peanut protein powder adds water to water content, the expanded 2min of twin-screw extruder, and outlet temperature is 110 DEG C, dry under 55 DEG C of conditions, is then crushed to 90 orders.
(3) once high-pressure homogeneous
The peanut protein powder pulverized adds the water of 3.5 times of weight, stirs fully and carries out homogeneous 5 times by high pressure homogenizer in pressure 22MPa, temperature 65 DEG C, form uniform peanut protein emulsion.
(4) enzymolysis polypeptide
To add the alkali protease accounting for its weight 2% in peanut protein emulsion, is 8.0 at pH value, and temperature is enzymolysis 5h under 55 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 8min that goes out, obtains polypeptide peanut protein emulsion after enzymolysis terminates;
(5) polypeptide debitterize
Adding in polypeptide peanut protein emulsion the papain accounting for its weight 1.5%, is 7.0 at pH value, and temperature is debittering by enzymatic hydrolysis 5h under 70 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 8min that goes out after enzymolysis terminates:
(6) secondary high-pressure homogeneous
Under pressure 20MPa, temperature 70 C condition, carry out second homogenate 6 times by high pressure homogenizer after debitterize terminates, form uniform peanut protein concentrate.
(7) spraying dry
Peanut protein concentrate is added the whey isolate protein accounting for its weight 1%, then carry out spraying dry, condition is: stream pressure is 0.25MPa, sample rate 7.2mL/s, EAT 190 DEG C, and leaving air temp 80 DEG C obtains high content of peptides peanut protein powder.
Embodiment 3
A processing method for high content of peptides peanut protein powder, comprises the following steps:
(1) degreasing
Peanut dregs is crushed to 70 orders, add the degreasing solvent be made up of according to mass ratio 2:1:0.3 cyclohexane, ethyl acetate and triethanolamine, the mass ratio of degreasing solvent and peanut dregs is 2.5:1,35min is stirred under 28 DEG C of conditions, vacuum filtration, cleaning, namely aeration-drying obtain defatted peanut protein powder;
(2) extrusion is pulverized
It is 40% that defatted peanut protein powder adds water to water content, the expanded 3min of twin-screw extruder, and outlet temperature is 120 DEG C, dry under 60 DEG C of conditions, is then crushed to 80 orders.
(3) once high-pressure homogeneous
The peanut protein powder pulverized adds the water of 3.5 times of weight, stirs fully and carries out homogeneous 4 times by high pressure homogenizer at pressure 25MPa, temperature 60 C, form uniform peanut protein emulsion.
(4) enzymolysis polypeptide
To add the alkali protease accounting for its weight 2% in peanut protein emulsion, is 8.0 at pH value, and temperature is enzymolysis 5h under 55 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 10min that goes out, obtains polypeptide peanut protein emulsion after enzymolysis terminates;
(5) polypeptide debitterize
Adding in polypeptide peanut protein emulsion the papain accounting for its weight 1.5%, is 7.0 at pH value, and temperature is debittering by enzymatic hydrolysis 5h under 70 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 5min that goes out after enzymolysis terminates:
(6) secondary high-pressure homogeneous
Under pressure 22MPa, temperature 65 DEG C of conditions, carry out second homogenate 5 times by high pressure homogenizer after debitterize terminates, form uniform peanut protein concentrate.
(7) spraying dry
Peanut protein concentrate is added the whey isolate protein accounting for its weight 1%, then carry out spraying dry, condition is: stream pressure is 0.25MPa, sample rate 7.2mL/s, EAT 190 DEG C, leaving air temp 80 DEG C, obtains high content of peptides peanut protein powder.
Comparative example 1
A processing method for peanut protein powder, step is with embodiment 1, and difference is: do not add triethanolamine during degreasing, does not add whey isolate protein during spraying dry, and other conditions are all with embodiment 1.
Comparative example 2
A processing method for peanut protein powder, step is with embodiment 1, and difference is: without extrusion, directly defatted peanut protein powder is crushed to 100 orders, and other steps are all with embodiment 1.
Effect test
1. the peanut protein powder prepared by embodiment 1-3 and comparative example 1-2 measures content of peptides, fatty residual volume and flour extraction, the assay method of the soy peptide powder provided according to GB/T22492-2008 standard carries out the mensuration of Peanut Polypeptide content, in the food provided according to GB/T5009.6-2003, the assay method of fat carries out the mensuration of fatty residual volume, and concrete outcome is in table 1.
Table 1
2. the peanut protein powder prepared by embodiment 1 and comparative example 1 the 6th, its color, smell outward appearance and coliform group count were detected in 12,18 months, concrete outcome is in table 2.
Table 2
3. the peanut protein powder prepared by embodiment 1-3 detects its oil absorption, water imbibition, foaming characteristic and foaming stability, and wherein, foaming characteristic detects according to dilation, foaming stability represents according to percentage of water loss, percentage of water loss is less, and stability is better, and concrete outcome is in table 3.
Table 3
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not by the restriction of embodiment; other is any do not deviate from Spirit Essence of the present invention and principle under make change, modification, combination, substitute, simplify and all should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (8)
1. a processing method for high content of peptides peanut protein powder, is characterized in that, comprise the following steps:
(1) degreasing
Peanut dregs is crushed to 70 orders, adds the degreasing solvent be made up of cyclohexane, ethyl acetate and triethanolamine, stirs 30-40min, vacuum filtration under 25-30 DEG C of condition, cleaning, and namely aeration-drying obtain defatted peanut protein powder;
(2) extrusion is pulverized
It is 30-40% that defatted peanut protein powder adds water to water content, the expanded 1-3min of twin-screw extruder, and outlet temperature is 100-120 DEG C, dry under 50-60 DEG C of condition, is then crushed to 80-100 order;
(3) once high-pressure homogeneous
The peanut protein powder pulverized adds the water of 3-4 times of weight, stirs fully and carries out homogeneous by high pressure homogenizer, form uniform peanut protein emulsion;
(4) enzymolysis polypeptide
Adding alkali protease by peanut protein emulsion, is 8.0 at pH value, and temperature is enzymolysis 5h under 55 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 5-10min that goes out, obtains polypeptide peanut protein emulsion after enzymolysis terminates;
(5) polypeptide debitterize
Adding papain by polypeptide peanut protein emulsion, is 7.0 at pH value, and temperature is debittering by enzymatic hydrolysis 5h under 70 DEG C of conditions, is warming up to the 100-105 DEG C of enzyme 5-10 min that goes out after enzymolysis terminates:
(6) secondary high-pressure homogeneous
Carry out homogeneous by high pressure homogenizer after debitterize terminates, form uniform peanut protein concentrate;
(7) spraying dry
Peanut protein concentrate is added whey isolate protein, then carries out spraying dry, obtain high content of peptides peanut protein powder.
2. processing method according to claim 1, is characterized in that, described degreasing solvent is 2-3:1 with peanut dregs mass ratio.
3. processing method according to claim 2, is characterized in that: described degreasing solvent is made up of according to mass ratio 2:1:0.3 cyclohexane, ethyl acetate and triethanolamine.
4. processing method according to claim 1, is characterized in that, described once high-pressure homogeneous and processing condition that is secondary high-pressure homogeneous is: pressure 20-25MPa, temperature 60-70 DEG C.
5. processing method according to claim 1, is characterized in that: the addition of described alkali protease accounts for 2% of peanut protein emulsion weight.
6. processing method according to claim 1, is characterized in that: the addition of described papain accounts for 1.5% of polypeptide peanut protein emulsion weight.
7. processing method according to claim 1, is characterized in that: the addition of described whey isolate protein accounts for 1% of peanut protein concentrate weight.
8. processing method according to claim 1, is characterized in that, described spray-dired condition is: stream pressure is 0.25 M Pa, sample rate 7.2 mL/s, EAT 190 DEG C, leaving air temp 80 DEG C.
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| CN105707409A (en) * | 2016-04-24 | 2016-06-29 | 山东玉皇粮油食品有限公司 | Low-bitterness instant peanut short peptides and preparation method thereof |
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| CN107048204A (en) * | 2016-12-05 | 2017-08-18 | 东北农业大学 | The method for preparing bean powder using soybean aqueous enzymatic method hydrolyzate |
| CN108456090A (en) * | 2018-06-29 | 2018-08-28 | 湖南泰谷生态工程有限公司 | A kind of lasting fragrance type bio-feritlizer and preparation method thereof |
| CN111265671A (en) * | 2020-01-21 | 2020-06-12 | 上海中医药大学 | Method for improving hot-melt wall adhesion of spray drying of traditional Chinese medicine or food raw materials |
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| CN105707409A (en) * | 2016-04-24 | 2016-06-29 | 山东玉皇粮油食品有限公司 | Low-bitterness instant peanut short peptides and preparation method thereof |
| CN107048204A (en) * | 2016-12-05 | 2017-08-18 | 东北农业大学 | The method for preparing bean powder using soybean aqueous enzymatic method hydrolyzate |
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Application publication date: 20150916 |