CN103250865A - Preparation method of peanut polypeptide - Google Patents
Preparation method of peanut polypeptide Download PDFInfo
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- CN103250865A CN103250865A CN 201310075965 CN201310075965A CN103250865A CN 103250865 A CN103250865 A CN 103250865A CN 201310075965 CN201310075965 CN 201310075965 CN 201310075965 A CN201310075965 A CN 201310075965A CN 103250865 A CN103250865 A CN 103250865A
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Abstract
The invention provides a preparation method of peanut polypeptide, comprising the following steps: a, processing of raw materials: uniformly mixing water and a peanut cake crushed material, which is obtained after a low-temperature cold-pressed peanut cake is crushed, so as to obtain a peanut cake suspension; b, homogenization of the suspension: homogenizing the peanut cake suspension to prepare a peanut cake mixed liquor; c, thermal-insulation enzymatic hydrolysis: adding a mixed the peanut cake mixed enzyme liquid of AlcaLase enzyme and papain into the peanut cake mixed liquor to carry out enzymatic hydrolysis at the temperature of 50-60 DEG C at pH of 7.0-8.5 for 6-8h so as to prepare a peanut cake enzymatic hydrolysate; d, high temperature sterilization: rapidly heating the peanut cake enzymatic hydrolysate to 90-95 DEG C; e, liquid-solid separation: carrying out liquid-solid separation on the peanut cake enzymatic hydrolysate which has undergone high-temperature enzyme deactivation and removing insoluble solids so as to prepare a peanut polypeptide liquid; f, condensation: condensing the peanut polypeptide liquid to prepare a peanut polypeptide concentrate; and g, drying: drying the peanut polypeptide concentrate so as to obtain a peanut polypeptide powder.
Description
Technical field
The invention belongs to food processing field, especially a preparation method who cultivates peanut polypeptide.
Background technology
Peanut has very high medical medical value, and it is malnutritive to can be used for treatment, taste imbalance, various anaemias, cough asthma due to excessive phlegm, the dry constipation of intestines, diseases such as agalactosis.Carry according to supplementary Amplifications of the Compendium of Materia Medica: peanut is pleased spleen and stomach, moistening lung for removing phlegm, nourish tonifying Qi, clearing is antipruritic." the southern regions of the Yunnan Province book on Chinese herbal medicine " carries: peanut salt solution is cooked and is controlled the lung pain, fries and uses the dryness-heat promoting circulation of blood, and it is painful to control the interior cold long-pending tripe of abdomen." property of medicine is examined " carries: this product fries edible clearing stomach and is amusing, and laxation is moisturized.Contain multiple anti-ageing composition in the peanut, especially outstanding with monounsaturated fatty acids, resveratrol, β-phytosterol, folic acid and 5 kinds of anti-ageing compositions of trace element zinc.These compositions suppress the improper aggegation of blood platelet, prevent cardiovascular and cerebrovascular disease that important function is arranged regulating function of human body.Peanut contains the people measures protein, and the amino acid of needed by human body can be provided.Peanut also contains nutritional labelings such as general coarse cereals rarely can promote human body metabolism, strengthen memory, intelligence development is lengthened the life choline, lecithin.Contain cyanidenon and B sitosterol in the peanut shell, these components are capable of lowering blood pressure and decreasing blood fat.Peanut red coat contains a large amount of VB
1, VB
2And be used for the tannin composition of antidiarrheal.The rich protein characteristic of the rich oil of peanut, nutritive value and health care have determined it will become the new focus of the international plant resources research and utilization in soybean exploitation back.
The peanut that China produces is except small part is edible as dry fruit, and major part is to be used for squeezing edible oil and fat as oil resource.Oil expression back gained accounts for that protein content is about 55% in the peanut dregs of peanut gross weight 56%～61%, and its amino acid is formed more complete, contains a large amount of essential amino acids, and nutritive value can compare favourably with the pattern protein that FAO recommends.And aboundresources, cheap, be the good plant protein resource that enzymolysis prepares biologically active polypeptide.Peanut dregs is used as feed or fertilizer more, abundant deep processing and utilization as yet, thereby cause the significant wastage of peanut protein resource.If peanut protein is carried out limited enzymolysis, can produce high nutrition, high added value and the biological products of the biologically active function that is easy to absorb.
The peanut polypeptide refers to peanut protein after the protease effect, again through the protein hydrolysate that process obtains such as separating, refining.Its total nitrogen all reaches more than 11.3 %, and molecular weight is below 26600, and is water-soluble all more than 90 %.There are some researches prove, peanut protein is the mixture of multiple little polypeptide through the product of protease A lcalase hydrolysis, its molecular weight is substantially in 300～2600 scopes, wherein molecular weight is at 98.5 % that account for below 1000, this part component is made up of the little polypeptide below 8 polypeptide, and many functional little polypeptide focus mostly in this section.
The peanut polypeptide has functional characteristic preferably, and the more peptide solution viscosity under high concentration is still lower as peanut, can keep dissolved state in wideer pH value scope; Have higher hygroscopicity and moisture retention; Osmotic pressure is more much lower than amino acid; Can CKIs matter form gel, reduce because the allergenicity that the big molecule of peanut protein causes has increased the stability of product etc.
Summary of the invention
Technical problem to be solved by this invention provides a production technology of cultivating peanut polypeptide powder, solved the degradation problem of high-fat peanut protein, can make its generation can be the direct peanut polypeptide powder that absorb, nutritious of human body the various nutritional labeling degradeds in the defatted peanut dregs of rice.
For solving the problems of the technologies described above the technical solution adopted in the present invention be:
One preparation method who cultivates peanut polypeptide, this preparation method may further comprise the steps:
A, raw material selected: normal with color, to be as good as the individual defatted peanut cake that goes mouldy be raw material;
The preliminary treatment of b, raw material: the groundnut meal particle after the defatted peanut cake pulverized and water mix and make the peanut cake suspension;
The homogeneous of c, suspension: peanut cake suspension homogeneous is made the peanut cake mixed liquor;
D, insulation enzymolysis: the mixed enzyme solution that adds AlcaLase enzyme and papain in the peanut cake mixed liquor, the addition of mixed enzyme solution is 0.03%～0.05% of peanut cake mixed liquor quality, enzyme hydrolysis condition is that temperature is 50～60 ℃, pH value 7.0～8.5,6～8 hours time, make the peanut cake enzymolysis liquid;
E, high-temperature sterilization: the temperature of peanut cake enzymolysis liquid is rapidly heated to 90～95 ℃, and is incubated 8～10 minutes;
Gu f, liquid-separation: Gu will carry out removing insoluble solid after liquid-separation through the go out peanut cake enzymolysis liquid of enzyme of high temperature, make the peanut polypeptide liquid;
G, concentrated: with the concentrated peanut polypeptide concentrate that makes of peanut polypeptide liquid;
H, drying: with peanut polypeptide concentrate drying, namely obtain the peanut polypeptide powder.
Among the step b, the defatted peanut cake pulverized with pulverizer and cross 40 mesh sieves after make the groundnut meal particle, make the peanut cake suspension after the ratio of 1:4～6 mixes by weight with water with the groundnut meal particle again.
Among the step c, the peanut cake suspension handled again making the peanut cake mixed liquor after by the colloid mill defibrination by the high pressure homogenizer homogeneous, homogenization pressure is 25～30Mpa.
In the steps d, the AlcaLase enzyme of mixed enzyme solution and the volume ratio of papain are AlcaLase enzyme: papain=1:1..
Among the step f, Gu adopt disk plate centrifuge that peach cake enzymolysis liquid is carried out liquid-separation.
In the step g, adopt vacuum to concentrate and make peanut polypeptide concentrate, the moisture of peanut polypeptide concentrate is 65～70%.
Among the step h, adopt spray-dired mode to make the peanut polypeptide powder.
The present invention compared with prior art has following advantage and effect:
(1) prepares the high-load rice bran protein with " the molten acid of alkali is heavy ", provide high-quality feedstocks for extracting the peanut biologically active polypeptide.
(2) adopt ultrafiltration that the peanut polypeptide is carried out classification and handle, guarantee the peanut polypeptide molecular weight of 95 % below 3 000 Da, and carry out secondary enzymolysis for the peanut polypeptide more than molecular weight 3 000 Da, improve utilization ratio of raw materials;
(3) utilize the negative and positive exchanger resin that peanut polypeptide crude product is carried out desalination, in desalination, also can remove the pigment composition in the peanut polypeptide, play the effect of decolouring;
(5) by the present invention preparation and refining peanut polypeptide, molecular weight is low, product stability good, has good antifatigue, effect such as hypotensive, anti-oxidant.
The specific embodiment
Embodiment
One preparation method who cultivates peanut polypeptide, this method comprises the steps:
The processing of a, raw material: the defatted peanut cake pulverized with pulverizer and cross 40 mesh sieves after make the groundnut meal particle, make the peanut cake suspension after the ratio of 1:4 mixes by weight with water with the groundnut meal particle again.
The homogeneous of b, suspension: the peanut cake suspension handled again making the peanut cake mixed liquor after by the colloid mill defibrination by the high pressure homogenizer homogeneous, homogenization pressure is 30Mpa.
C, insulation enzymolysis: add the mixed enzyme solution of AlcaLase enzyme and papain in the peanut cake mixed liquor,
The AlcaLase enzyme of mixed enzyme solution and the volume ratio of papain are the AlcaLase enzyme: the addition of papain=1:1. mixed enzyme solution is 0.05% of peanut cake mixed liquor quality, enzyme Sui Jie condition is that temperature is 60 ℃, pH value 7.0,8 hours time, make the peanut cake enzymolysis liquid;
D, high-temperature sterilization: the temperature of peanut cake enzymolysis liquid is rapidly heated to 95 ℃, and is incubated 10 minutes;
Gu e, liquid-separation: Gu will carry out removing insoluble solid after liquid-separation through the go out peanut cake enzymolysis liquid of enzyme of high temperature, make the peanut polypeptide liquid;
F, concentrated: adopt vacuum to concentrate the peanut polypeptide liquid and make peanut polypeptide concentrate, the moisture of peanut polypeptide concentrate is 70%.Concentrate and make peanut polypeptide concentrate;
G, drying: adopt spray-dired mode to make the peanut polypeptide powder peanut polypeptide concentrate.
Claims (7)
1. a preparation method who cultivates peanut polypeptide is characterized in that comprising the steps:
The processing of a, raw material: the groundnut meal particle after the pulverizing of low-temperature cold pressing defatted peanut cake and water are mixed worth peanut cake suspension;
The homogeneous of b, suspension: peanut cake suspension homogeneous is made the peanut cake mixed liquor;
C, insulation enzymolysis: add the mixed enzyme solution of AlcaLase enzyme and papain in the peanut cake mixed liquor, the addition of mixed enzyme solution is 0.03%～0.05% of peanut cake mixed liquor quality, enzyme Sui Jie condition be temperature be 50～60 ℃, pH value 7.0～
8.5,6～8 hours time, make the peanut cake enzymolysis liquid;
D, high-temperature sterilization: the temperature of peanut cake enzymolysis liquid is rapidly heated to 90～95 ℃, and is incubated 8～10 minutes;
Gu e, liquid-separation: Gu will carry out removing insoluble solid after liquid-separation through the go out peanut cake enzymolysis liquid of enzyme of high temperature, make the peanut polypeptide liquid;
F, concentrated: with the concentrated peanut polypeptide concentrate that makes of peanut polypeptide liquid;
G, drying: with peanut polypeptide concentrate drying, namely obtain the peanut polypeptide powder.
2. according to claim 1 one preparation method who cultivates peanut polypeptide is characterized in that: among the step b, the defatted peanut cake pulverized with pulverizer and cross 40 mesh sieves after make the groundnut meal particle, again with groundnut meal particle and water by weight 1:4～
After mixing, 6 ratio makes the peanut cake suspension.
3. according to claim 1 one preparation method who cultivates peanut polypeptide is characterized in that: among the step c, the peanut cake suspension handled again making the peanut cake mixed liquor after by the colloid mill defibrination by the high pressure homogenizer homogeneous, homogenization pressure is 25～30Mpa.
4. according to claim 1 one preparation method who cultivates peanut polypeptide, it is characterized in that: in the steps d, the AlcaLase enzyme of mixed enzyme solution and the volume ratio of papain are AlcaLase enzyme: papain=1:1.
5. according to claim 1 one preparation method who cultivates peanut polypeptide is characterized in that: among the step f, Gu adopt disk plate centrifuge that peach cake enzymolysis liquid is carried out liquid-separation.
6. according to claim 1 one preparation method who cultivates peanut polypeptide, it is characterized in that: in the step g, adopt vacuum to concentrate and makes peanut polypeptide concentrate, the moisture of peanut polypeptide concentrate is 65～70%.
7. according to claim 1 one preparation method who cultivates peanut polypeptide is characterized in that: among the step h, adopt spray-dired mode to make the peanut polypeptide powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310075965 CN103250865A (en) | 2013-03-11 | 2013-03-11 | Preparation method of peanut polypeptide |
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| CN 201310075965 CN103250865A (en) | 2013-03-11 | 2013-03-11 | Preparation method of peanut polypeptide |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478403A (en) * | 2013-09-06 | 2014-01-01 | 江苏丘陵地区镇江农业科学研究所 | Peanut polypeptide and preparation method thereof |
| CN103622878A (en) * | 2013-12-12 | 2014-03-12 | 权威宇 | Night cream containing peanut polypeptide and preparation method of night cream |
| CN103734319A (en) * | 2013-12-19 | 2014-04-23 | 华中农业大学 | Peanut milk nutrient solution prepared from peanut meal protein extracting solution |
| CN103966264A (en) * | 2013-01-24 | 2014-08-06 | 金利食安科技股份有限公司 | Method for extracting hydrolysate of non-animal component by combining pressure and enzyme |
| CN104904988A (en) * | 2015-06-17 | 2015-09-16 | 山东省农业科学院农产品研究所 | Method for processing peanut protein powder with high polypeptide content |
| CN105192244A (en) * | 2015-09-29 | 2015-12-30 | 潜山县传文瓜子有限公司 | Method for preparing snakegourd fruit seed polypeptides |
| CN108300751A (en) * | 2018-03-19 | 2018-07-20 | 广西南宁人人想食品有限公司 | A kind of method of peanut meal extraction Peanut Polypeptide |
| CN111513289A (en) * | 2020-05-15 | 2020-08-11 | 杭州耐雀生物科技有限公司 | Compound nut concentrated solution and production process and application thereof |
-
2013
- 2013-03-11 CN CN 201310075965 patent/CN103250865A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966264A (en) * | 2013-01-24 | 2014-08-06 | 金利食安科技股份有限公司 | Method for extracting hydrolysate of non-animal component by combining pressure and enzyme |
| CN103478403A (en) * | 2013-09-06 | 2014-01-01 | 江苏丘陵地区镇江农业科学研究所 | Peanut polypeptide and preparation method thereof |
| CN103622878A (en) * | 2013-12-12 | 2014-03-12 | 权威宇 | Night cream containing peanut polypeptide and preparation method of night cream |
| CN103734319A (en) * | 2013-12-19 | 2014-04-23 | 华中农业大学 | Peanut milk nutrient solution prepared from peanut meal protein extracting solution |
| CN104904988A (en) * | 2015-06-17 | 2015-09-16 | 山东省农业科学院农产品研究所 | Method for processing peanut protein powder with high polypeptide content |
| CN105192244A (en) * | 2015-09-29 | 2015-12-30 | 潜山县传文瓜子有限公司 | Method for preparing snakegourd fruit seed polypeptides |
| CN108300751A (en) * | 2018-03-19 | 2018-07-20 | 广西南宁人人想食品有限公司 | A kind of method of peanut meal extraction Peanut Polypeptide |
| CN111513289A (en) * | 2020-05-15 | 2020-08-11 | 杭州耐雀生物科技有限公司 | Compound nut concentrated solution and production process and application thereof |
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Application publication date: 20130821 |