CN103622878A - Night cream containing peanut polypeptide and preparation method of night cream - Google Patents
Night cream containing peanut polypeptide and preparation method of night cream Download PDFInfo
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- CN103622878A CN103622878A CN201310672873.2A CN201310672873A CN103622878A CN 103622878 A CN103622878 A CN 103622878A CN 201310672873 A CN201310672873 A CN 201310672873A CN 103622878 A CN103622878 A CN 103622878A
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- polypeptide
- peanut
- enzymolysis
- late frost
- peanut polypeptide
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 130
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 110
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 109
- 235000017060 Arachis glabrata Nutrition 0.000 title claims abstract description 94
- 241001553178 Arachis glabrata Species 0.000 title claims abstract description 94
- 235000010777 Arachis hypogaea Nutrition 0.000 title claims abstract description 94
- 235000018262 Arachis monticola Nutrition 0.000 title claims abstract description 94
- 235000020232 peanut Nutrition 0.000 title claims abstract description 94
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000006071 cream Substances 0.000 title abstract 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 40
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000012153 distilled water Substances 0.000 claims abstract description 26
- 239000003381 stabilizer Substances 0.000 claims abstract description 23
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims description 27
- 102000004190 Enzymes Human genes 0.000 claims description 27
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- 210000000582 semen Anatomy 0.000 claims description 27
- 230000007062 hydrolysis Effects 0.000 claims description 25
- 238000006460 hydrolysis reaction Methods 0.000 claims description 25
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- 238000000034 method Methods 0.000 claims description 23
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 21
- 230000002421 anti-septic effect Effects 0.000 claims description 20
- 238000012545 processing Methods 0.000 claims description 20
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- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims description 7
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 4
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- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of cosmetics, particularly relates to a night cream containing a peanut polypeptide, and further relates to a preparation method of the night cream. The night cream comprises the following components: the peanut polypeptide, rose essential oil, a humectant, a penetrant, a preservative, a stabilizer, and distilled water or normal saline. The night cream and the preparation method have the benefits that a peanut protein, which has a bad smell, is poor in water solubility and is not easily absorbed by the skin, is hydrolyzed into the peanut polypeptide, and then the peanut polypeptide is mixed with other components to obtain the night cream, the defects of the peanut protein and the peanut polypeptide are overcome, and the prepared night cream has no bad smell, is easily absorbed by the skin, can promote the cell growth, differentiation and proliferation, and keeps the elasticity and the quality of the skin.
Description
Technical field
The invention belongs to cosmetics technical field, be specifically related to a kind of late frost containing Peanut Polypeptide, also relate to the preparation method of this late frost.
background technology
Semen arachidis hypogaeae protein is the vegetable protein that a kind of nutritive value is very high, and the immunological enhancement function that its biological value (BV is 5, and efficiency value is 11) contains needed by human body, to safeguarding that health and early children development have important function.Research is found, the peptide that protein makes through hydrolysis has than the better nutritional properties of protein, experiment confirms that the absorbance of peptide is higher than aminoacid, than aminoacid more easily sooner by the small intestinal mucosa utilization that is absorbed by the body, Semen arachidis hypogaeae protein active polypeptide has the physiological function of multiple brilliance in nourishing healthy field, the one, polypeptide can directly be absorbed without degraded by intestinal, infiltration rate and absorbance are all higher than protein and aminoacid, thereby the form that can be used as enteral nutrition agent and spoon meat offers the crowd under special health; The 2nd, it not only provides human body necessary nutrient protein, can also effectively reduce the content of Blood Cholesterol and triglyceride, accelerate power consumption in body, burning fat, opposing is fat, the 3rd, peanut protein polypeptide can provide abundant aminoacid for human body, promotes protein synthesis, suppresses ribonuclease activity and declines, remove free radical and heavy metal in human body, improve cellular metabolism, for the antibody of immune system preparation to antibacterium and infection, improve immune function of human body.
Different from other polypeptide, Peanut Polypeptide contains resveratrol, and it has obvious inhibitory action to cardiovascular disease and arteriosclerosis, also can promote whereby human tumor cell's apoptosis, or is subject to the performance of tumor-inhibiting factor to reach anti-tumor activity.Therefore China has strengthened the development and utilization to Peanut Polypeptide in recent years.
The production of polypeptide mainly contains synthetic and two kinds of methods of protein extracorporeal hydrolysis.Synthetic method mainly contains chemosynthesis, DNA recombinant technique is synthetic, and enzyme process synthesizes three kinds of methods.Although the synthetic any bioactive peptide of the wish that synthetic method can amine people, at present because cost is high, more than side reaction thing and the problem such as residue restricting its development.If recombinant DNA technology is expected to successfully, will hold out broad prospects, extracorporeal hydrolysis protein is that peptide has three kinds of methods, acid hydrolysis, basic hydrolysis and enzymatic hydrolysis, first two method is simple to operation, but may be in harmful effect aspect nutrition and toxicity, therefore its application has been subject to certain restriction, protease hydrolysis can position under certain conditions hydrolysis and produce specific peptide, and be easy to the process of controlled hydrolysis, and have height specificity, generally can not cause the loss of nutrition aspect, can under gentle condition, carry out, energy consumption is low, thereby can meet preferably peptide need of production.
Peanut Polypeptide solution viscosity under high concentration is still lower, can within the scope of wider pH value, keep dissolved state, there is higher hygroscopicity and moisture retention, osmotic pressure is more much lower than aminoacid, can Profilin matter form gel, reduce the allergenicity causing due to Semen arachidis hypogaeae protein macromole, increased the stability of product.
China starts late in the research that utilizes protease to prepare aspect biologically active peptide, in early days proteolysis research is mainly concentrated on and improves protein mill properties and Peanut Polypeptide aspect, and it is just at the early-stage for the research of peanut bioactive peptide, Related product is few, conventionally only comparatively general in the application in nourishing healthy field.
Peanut Polypeptide has several large advantages:
Purification purity is high, and according to detection, the content of effective ingredient activated protein peptide is 9%;
Price has heat transfer, compares with some polypeptide, and the price of Semen arachidis hypogaeae protein peptide is comparatively cheap;
In Semen arachidis hypogaeae protein, 96% albumen belongs to globulin, higher than other albumen;
Not containing the peculiar antinutritional factor of leguminous plant, free from extraneous odour;
In Semen arachidis hypogaeae protein peptide, contain resveratrol, cardiovascular disease and arteriosclerosis, promotion human tumor cell's apoptosis.
If peanut protein polypeptide is applied to cosmetics, its distinctive character will be brought beyond thought effect for numerous women's skin protection.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides that a kind of irritated rate is low, the reservation of the larger limit of active component and be easy to stop absorbed late frost containing Peanut Polypeptide and preparation method thereof for people.
The present invention realizes by following technical scheme:
Containing the late frost of Peanut Polypeptide, comprise the component of following portions by weight:
Peanut Polypeptide 5-20 part
Flos Rosae Rugosae quintessence oil 1-2 part
Wetting agent 1-4 part
Penetrating agent 1-3 part
Antiseptic 0.05-0.2 part
Stabilizing agent 1-3 part
Distilled water or normal saline 5-80 part.
Preferably, a kind of late frost containing Peanut Polypeptide, comprises the component of following portions by weight:
Peanut Polypeptide 8-18 part
Flos Rosae Rugosae quintessence oil 1-2 part
Wetting agent 1-3 part
Penetrating agent 1-2 part
Antiseptic 0.06-0.12 part
Stabilizing agent 1-2 part
Distilled water or normal saline 10-80 part.
Preferred, a kind of late frost containing Peanut Polypeptide, comprises the component of following portions by weight:
15 parts of Peanut Polypeptides
2 parts of Flos Rosae Rugosae quintessence oils
1 part of wetting agent
1 part of penetrating agent
0.1 part of antiseptic
1 part of stabilizing agent
50 parts of distilled water or normal saline.
Wetting agent is glycerol, 1, at least one in 3-butanediol, hyaluronic acid, propylene glycol, vaseline, Trivent OCG, glucosan glycosides, poly-glycosaminoglycan, silk polypeplide and lecithin;
Penetrating agent is one or both in azone and dextran sulfate;
Antiseptic is at least one in methyl parahydroxybenzoate, propyl p-hydroxybenzoate, isopropyl alcohol and propylene glycol;
Stabilizing agent is at least one in Polyethylene Glycol, cetostearyl alcohol, HANSHENGJIAO, CSSO3, methylol aminopolysaccharide, sulphuric acid mannan, dextran sulfate, polyglycine, glutathion and half amide acid, glyceryl stearate+PEG-100 stearate and cetearyl alcohol acid alcohol+cetearyl alcohol acidic group glucoside.
As a modification of the present invention, containing the late frost of Peanut Polypeptide, comprise the component of following portions by weight:
15 parts of Peanut Polypeptides
2 parts of Flos Rosae Rugosae quintessence oils
1 part of hyaluronic acid
1 part of dextran sulfate
0.1 part of isopropyl alcohol
1 part of glutathion
50 parts of distilled water.
A preparation method that contains the late frost of Peanut Polypeptide, comprises following step:
(1) pretreatment of Semen arachidis hypogaeae protein
Get Semen arachidis hypogaeae dregs, by Semen arachidis hypogaeae dregs ungrease treatment;
(2) two step enzymolysis are prepared Peanut Polypeptide
With pure water, adjust concentration of substrate 10%-15%, papain enzyme concentration 1%-3%, 45 ℃-60 ℃ of hydrolysis temperatures, enzymolysis time 2-4h, pH value 6.5-8.0,80 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 8.0-11.0, add the Esperase enzymolysis 2-4h of 1%-3%, 45 ℃-60 ℃ of hydrolysis temperatures, 90 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3%-5% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby.
(3) preparation of late frost
Get Peanut Polypeptide 5-20 part, Flos Rosae Rugosae quintessence oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.2 part, stabilizing agent 1-3 part, distilled water or normal saline 5-80 part standby;
Add container for stirring to heat to 70-90 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;
Above-mentioned raw material and distilled water or normal saline are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2500-2800 γ/min; Temperature is 60-80 ℃, and persistent period 30-45 minute treats that homogenizing is cooled to 35-40 ℃, adds Flos Rosae Rugosae quintessence oil and Peanut Polypeptide, and keeping temperature is 35 ℃, continues to stir 10-20 minute, and bottling, sterilizing, be finished product after cooling.
Degreasing method is:
In step (1), adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:5-1:10, and skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood; The organic solvent of defat is any in normal hexane, absolute ether, ethanol, acetone, dichloromethane.
Or adopt supercritical carbon dioxide extraction method to process defatting peanut, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min.
Adopt the mode of supercritical carbon dioxide extraction to remove oils and fats, its advantage is not introduce organic solvent, and the oils and fats that adopts the method extraction to obtain does not have dissolvent residual, can not bring pollution to product, can not make product rotten yet, can obtain containing the low Semen arachidis hypogaeae albumen powder of oils and fats.
Preferably, contain the preparation method of the late frost of Peanut Polypeptide, comprise following step:
(1) pretreatment of Semen arachidis hypogaeae protein
Adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:8, and 37 ℃ of skimming temps, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
Or adopt supercritical carbon dioxide extraction method to process defatting peanut, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min;
(2) two step enzymolysis are prepared Peanut Polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% Esperase enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby.
(3) preparation of late frost
Get Peanut Polypeptide 5-20 part, Flos Rosae Rugosae quintessence oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.2 part, stabilizing agent 1-3 part, distilled water or normal saline 5-80 part standby;
Add container for stirring to heat to 80 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;
Above-mentioned raw material and distilled water or normal saline are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add Flos Rosae Rugosae quintessence oil and Peanut Polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
The container that holds late frost is glass container or rustless steel container, because contain the composition of quintessence oil in late frost, Flos Rosae Rugosae quintessence oil its active component in thermal environment plastics environment is easily lost, and therefore adopts glass container to hold.
Semen arachidis hypogaeae protein solidifies precipitation under acid condition, and particularly near isoelectric point, IP, dissolubility sharply declines, and peanut peptide can keep higher dissolubility.Because human body skin adapts to slightly acidic environment, so many cosmetics have added the acidic materials such as fruit acid class, for these type of cosmetics, the interpolation of peanut peptide is the unlikely precipitation that causes also, and can be good at bringing into play the effect of its application.
Peanut peptide has very strong hygroscopicity and moisture retention, this specific character makes Peanut Polypeptide can act on human body skin owing to containing a large amount of globulin in Semen arachidis hypogaeae protein, the poorly water-soluble of globulin, directly applied to cosmetics for example in the production of late frost, in globulin and cosmetics, other composition is as very difficult mixing such as wetting agent, stabilizing agents, even merge preferably with above-mentioned material, also be difficult for being absorbed by skin, its active component can not be utilized by skin, can not play the effect that improves the skin quality colour of skin.
In addition, because part population is irritated to the Semen arachidis hypogaeae protein of high protein, as directly Semen arachidis hypogaeae protein being applied in the production of late frost, its irritated factor is not still eliminated, and the composition that easily causes skin allergy containing in the product occurring with cosmetics form still can continue to make skin allergy;
Therefore, in order to address this problem, the present invention is hydrolyzed to micromolecular polypeptide by Semen arachidis hypogaeae protein, the cosmetics of producing with polypeptide form are late frost for example, its performance has obtained larger change, water solublity strengthens greatly, is easy to produce and be easy to the cosmetics that absorb for human body skin with other the conventional adjuvant of cosmetics;
Due to the micromolecule polypeptide after hydrolysis, be different from the high protein of original macromolecule in addition, take micromolecule polypeptide as the cosmetics that raw material prepares, eliminated irritated factor, use crowd no longer produces allergic phenomena.
The peanut protein polypeptide that hydrolysis obtains has promotion cell differentiation, promote epidermis healing, increase the synthetic and secretion of extracellular matrix, promote capillary of skin net and little vascularization, the anti-ageing various biological effect of waiting for a long time, in research in anti-aging cosmetics produces, over the years biologically active polypeptide is comprised to cell growth factor particularly payes attention to, this class material is very micro-at people's in-vivo content, but biological activity is high, in various kinds of cell physiological function and metabolic activity, play important physiological regulatory action, affect directly or indirectly the growth of cell, division, differentiation, propagation and migration.
Although Semen arachidis hypogaeae protein is hydrolyzed to polypeptide, but this polypeptide has still retained some characteristic of former albumen, for example, perishability, owing to containing bata-phenethyl alcohol and eugenol in Flos Rosae Rugosae quintessence oil, this two material has antibacterial and depression and antiseptic property preferably, has overcome the easily perishable defect of raw bacterium of Peanut Polypeptide;
It is active that the present invention makes full use of the strong anti-oxidation of natural flowers raw albumen enzymatic hydrolysate, be applied in skin-protection product, be applied to feedstuff in compare, improved it and be worth, also improved its pair value added.
Beneficial effect of the present invention is, to there be abnormal flavour, poorly water-soluble, be not easy to be hydrolyzed to Peanut Polypeptide for the Semen arachidis hypogaeae protein that skin absorbs, again Peanut Polypeptide is mixed mutually with other component, be prepared into late frost, overcome the defect that HUASHENGDAN Pseudobulbus Bletillae (Rhizoma Bletillae) Peanut Polypeptide itself has, prepared the late frost free from extraneous odour of gained, easily by skin, absorbed, energy Promote cell's growth, differentiation, propagation, elasticity and the skin quality of maintenance skin.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
(1) processing of raw material
Get hot pressed peanut meal, ungrease treatment, concrete steps are: adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:8,37 ℃ of skimming temps, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
(2) two step enzymolysis are prepared Peanut Polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% Esperase enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby;
(3) preparation of late frost
Add container for stirring to heat to 70-90 ℃ 40 parts of 1 part of hyaluronic acids, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, stabilizing agent, 1 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 15 parts of 2 parts of Flos Rosae Rugosae quintessence oils and Peanut Polypeptides, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
Comparative example 1
(1) processing of raw material
Get hot pressed peanut meal, ungrease treatment, concrete steps are: adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:8,37 ℃ of skimming temps, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
(2) preparation of late frost
Add container for stirring to heat to 80 ℃ 40 parts of 3 parts of hyaluronic acids, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, stabilizing agent, 1 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water or normal saline are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 15 parts of Flos Rosae Rugosae quintessence oil and Peanut Polypeptides, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
With the difference of embodiment 1 be, only by Semen arachidis hypogaeae protein pretreatment, after pulverizing again after pulverizing defat, as raw material, do not comprise that with other adjuvant Flos Rosae Rugosae quintessence oil combines, prepare late frost.
Comparative example 2
(1) processing of raw material
Get hot pressed peanut meal, ungrease treatment, concrete steps are: adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:8,37 ℃ of skimming temps, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
(2) enzymolysis is prepared Peanut Polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% Esperase enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby.
(3) preparation of late frost
Add container for stirring to heat to 80 ℃ 40 parts of 3 parts of hyaluronic acids, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, 1 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 15 parts of Peanut Polypeptides, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
, in comparative example 2, not add Flos Rosae Rugosae quintessence oil with the difference of embodiment 1; Hyaluronic ratio is increased to 2 weight portions, and all the other are identical;
Human body is used experimentation containing the improvement effect of Peanut Polypeptide late frost to cortex wrinkle
Study subject and use
60 of female volunteers, age 23-50 year, be divided at random three groups, 1 group of 20 people of embodiment, comparative example 1, 2 groups of each 20 people, duration of test, the inactive any external thing in test position and/or cosmetics, experimenter is respectively by given the test agent and comparative example 1, 2 samples are applied to respectively left and right cheek and canthus, after getting up early every day and evening sleep before each once, continuous 8 weeks, adopt silicone rubber film coating method to copy the wrinkle of skin at the tested cheek of experimenter and position, canthus, numbering, perusal, evaluation criteria is according to the rules improved and is marked wrinkle degree, scoring group is comprised of 2 people, adopt double-blind method.
Result is as shown in the table:
The user that wrinkle depth improvement number smooths for wrinkle before and after using;
Wrinkle depth improves %=(use before-wrinkle depth after using)/wrinkle depth before using;
The average effective integration that smooths is high shows that formula is obvious to wrinkle improvement effect, is less than 0 for invalid.
In embodiment 1, late frost has the fragrance of pleasant, and exquisiteness is applied to skin, and skin absorbs fast, keeps immediately humidity after can be observed surface skin moistening, and skin surface is without coating;
In comparative example 1, late frost has abnormal flavour, coarse, is applied to skin, and skin is not easy to absorb, and forms the obvious coating of one deck on surface;
In comparative example 2, late frost has lighter abnormal flavour, and exquisiteness is applied to skin, and skin absorbs fast, keeps immediately humidity after can be observed surface skin moistening, and skin surface is without coating.
From above phenomenon, can find out, Flos Rosae Rugosae quintessence oil is collaborative mutually with Peanut Polypeptide, and covered Semen arachidis hypogaeae protein has abnormal flavour residual in peptide more, replaces as allowing the joyful fragrance of people;
From above, can find out relatively, peanut protein polypeptide is late frost prepared by raw material, the good absorbing effect of skin, take effect obviously, effectively improved the wrinkle depth of skin of face, have potential value, Peanut Polypeptide can promote and accelerate Growth of Cells, propagation, synthetic protein or peptide material, aspect alleviation skin aging and skin trauma reparation, is playing an important role.
Embodiment 2
(1) processing of raw material
Adopt supercritical carbon dioxide extraction method to process defatting peanut, the condition of extraction is: extraction temperature is 45 ℃, and pressure is 25Mpa, time 180min;
(2) enzymolysis is prepared Peanut Polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% Esperase enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby.
(3) preparation of late frost
Add container for stirring to heat to 80 ℃ of left and right 40 parts of 1 part of glycerol, 1 part of azone, 0.1 part of propyl p-hydroxybenzoate, 0.5 part of Polyethylene Glycol, 0.5 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add Flos Rosae Rugosae quintessence oil and Peanut Polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
Embodiment 3
(1) processing of raw material
Adopt supercritical carbon dioxide extraction method to process defatting peanut, the condition of extraction is: extraction temperature is 45 ℃, and pressure is 25Mpa, time 180min;
(2) enzymolysis is prepared Peanut Polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% Esperase enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby.
(3) preparation of late frost
Add container for stirring to heat to 80 ℃ of left and right 50 parts of 1 part of glycerol, 1 part of azone, 0.1 part of propyl p-hydroxybenzoate, 0.5 part of Polyethylene Glycol, 0.5 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 5 parts of Flos Rosae Rugosae quintessence oil 1 and Peanut Polypeptides, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
In the present embodiment, adopt papain and these two kinds of enzyme enzymolysis Semen arachidis hypogaeae proteins of Esperase enzyme, the peptide of micromolecule amount (molecular weight is less than 1000Da) accounts for 35.6% of total peptide content;
If adopt the papain enzymolysis Semen arachidis hypogaeae protein of same amount, the peptide of micromolecule amount (molecular weight is less than 1000Da) accounts for 31.1% of total peptide content;
And in peanut protein polypeptide, the polypeptide that plays physiological activity is mainly micromolecule amount, particularly molecular weight is less than the polypeptide of 1500 Da, if the content of peptides of micromolecule amount is higher, the physiological active functions of peanut protein polypeptide is also stronger.
From above, can find out relatively, two kinds of enzyme effects in the present embodiment are better than only adopting the consumption of the papain of same amount.
Embodiment 4
Be from the different of embodiment 1, wetting agent is 1 part of glucosan glycosides, 1 part of poly-glycosaminoglycan;
Penetrating agent is 1 part of azone;
Antiseptic is 0.1 part of methyl parahydroxybenzoate;
Stabilizing agent is 1 part of glutathion, 1 part of half amide acid;
All the other are identical.
Embodiment 5
Wetting agent is 1 part of glycerol, 1 part of silk polypeplide;
Penetrating agent is 1 part of azone and dextran sulfate;
Antiseptic is 0.1 part of methyl parahydroxybenzoate;
Stabilizing agent is 2 parts of methylol aminopolysaccharides.
All the other are identical.
Embodiment 6
Wetting agent is 2 parts, lecithin;
Penetrating agent is 1 part of azone;
Antiseptic is 0.02 part of propyl p-hydroxybenzoate;
Stabilizing agent is 1 part of polyglycine, 1 part of half amide acid.
All the other are identical.
Embodiment 7
Wetting agent is 1 part of glucosan glycosides, 1 part of poly-glycosaminoglycan;
Penetrating agent is 2 parts of azones;
Antiseptic is 0.1 part of methyl parahydroxybenzoate;
Stabilizing agent is 1 part of poly-dextran sulfate, 1 part of polyglycine.
All the other are identical.
Claims (9)
- Containing the late frost of Peanut Polypeptide, comprise the component of following portions by weight:Peanut Polypeptide 5-20 partFlos Rosae Rugosae quintessence oil 1-2 partWetting agent 1-4 partPenetrating agent 1-3 partAntiseptic 0.05-0.2 partStabilizing agent 1-3 partDistilled water or normal saline 5-80 part.
- 2. a kind of late frost containing Peanut Polypeptide as claimed in claim 1, is characterized in that, described late frost comprises the component of following portions by weight:Peanut Polypeptide 8-18 partFlos Rosae Rugosae quintessence oil 1-2 partWetting agent 1-3 partPenetrating agent 1-2 partAntiseptic 0.06-0.12 partStabilizing agent 1-2 partDistilled water or normal saline 10-80 part.
- 3. a kind of late frost containing Peanut Polypeptide as claimed in claim 1, is characterized in that, described late frost comprises the component of following portions by weight:15 parts of Peanut Polypeptides2 parts of Flos Rosae Rugosae quintessence oils1 part of wetting agent1 part of penetrating agent0.1 part of antiseptic1 part of stabilizing agent50 parts of distilled water or normal saline.
- 4. a kind of late frost containing Peanut Polypeptide as described in any one in claim 1-3, it is characterized in that, described wetting agent is glycerol, 1, at least one in 3-butanediol, hyaluronic acid, propylene glycol, vaseline, Trivent OCG, glucosan glycosides, poly-glycosaminoglycan, silk polypeplide and lecithin;Described penetrating agent is one or both in azone and dextran sulfate;Described antiseptic is at least one in methyl parahydroxybenzoate, propyl p-hydroxybenzoate, isopropyl alcohol and propylene glycol;Described stabilizing agent is at least one in Polyethylene Glycol, cetostearyl alcohol, HANSHENGJIAO, CSSO3, methylol aminopolysaccharide, sulphuric acid mannan, dextran sulfate, polyglycine, glutathion and half amide acid, glyceryl stearate+PEG-100 stearate and cetearyl alcohol acid alcohol+cetearyl alcohol acidic group glucoside.
- 5. a kind of late frost containing Peanut Polypeptide as claimed in claim 4, is characterized in that, described late frost comprises the component of following portions by weight:15 parts of Peanut Polypeptides2 parts of Flos Rosae Rugosae quintessence oils1 part of hyaluronic acid1 part of dextran sulfate0.1 part of isopropyl alcohol1 part of glutathion50 parts of distilled water.
- 6. the preparation method of a kind of late frost containing Peanut Polypeptide as claimed in claim 1, comprises following step:(1) pretreatment of Semen arachidis hypogaeae proteinGet Semen arachidis hypogaeae dregs, by Semen arachidis hypogaeae dregs ungrease treatment;(2) two step enzymolysis are prepared Peanut PolypeptideWith pure water, adjust concentration of substrate 10%-15%, acid protease enzyme concentration 1-3%, hydrolysis temperature 45-55 ℃, enzymolysis time 2-4h, pH value 2.3-4,90-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;After first step enzymolysis completes, regulate enzymolysis solution pH to 8.0-11.0, add the enzymolysis 2-4h of 1%-3%, 45 ℃-60 ℃ of hydrolysis temperatures, 90 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;Gained polypeptide solution adds 3-5% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby;(3) preparation of late frostAdd container for stirring to heat to 70-90 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;Above-mentioned raw material and distilled water or normal saline are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2500-2800 γ/min; Temperature is 60-80 ℃, and persistent period 30-45 minute treats that homogenizing is cooled to 35-40 ℃, adds Flos Rosae Rugosae quintessence oil and Peanut Polypeptide, and keeping temperature is 35 ℃, continues to stir 10-20 minute, and bottling, sterilizing, be finished product after cooling.
- 7. the preparation method of a kind of late frost containing Peanut Polypeptide as claimed in claim 1, is characterized in that, in described step (1), the organic solvent of defat is any in normal hexane, absolute ether, ethanol, acetone, dichloromethane.
- 8. the preparation method of a kind of late frost containing Peanut Polypeptide as claimed in claim 1, is characterized in that, described degreasing method is:Adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:5-1:10, and skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;Or adopt supercritical carbon dioxide extraction method to process defatting peanut, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min.
- 9. the preparation method of a kind of late frost containing Peanut Polypeptide as described in any one in claim 6-8, comprises following step:(1) pretreatment of Semen arachidis hypogaeae proteinTake Semen arachidis hypogaeae dregs as raw material, adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:8, and 37 ℃ of skimming temps, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;Or adopt supercritical carbon dioxide extraction method to process defatting peanut, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min;(2) two step enzymolysis are prepared Peanut PolypeptideWith pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% Esperase enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains Peanut Polypeptide dry powder, standby;(3) preparation of late frostGet Peanut Polypeptide 5-20 part, Flos Rosae Rugosae quintessence oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.2 part, stabilizing agent 1-3 part, distilled water or normal saline 5-80 part standby;Add container for stirring to heat to 80 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;Above-mentioned raw material and distilled water or normal saline are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add Flos Rosae Rugosae quintessence oil and Peanut Polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310672873.2A CN103622878B (en) | 2013-12-12 | 2013-12-12 | A kind of late frost containing Peanut Polypeptide and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104530197A (en) * | 2015-01-22 | 2015-04-22 | 马淑娟 | Water retention peptide extracted from salix mongolica and application of water retention peptide in cosmetics |
| CN109846746A (en) * | 2019-02-14 | 2019-06-07 | 重庆市洲仨科技发展有限公司 | A kind of compound maintenance polypeptide of natural activity and preparation method thereof |
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| CN101433510A (en) * | 2008-12-10 | 2009-05-20 | 庆阳市康惠玫瑰发展有限责任公司 | Rose essential oil skin-moistening lotion and preparation method thereof |
| CN103250865A (en) * | 2013-03-11 | 2013-08-21 | 武汉工业学院 | Preparation method of peanut polypeptide |
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| CN101433510A (en) * | 2008-12-10 | 2009-05-20 | 庆阳市康惠玫瑰发展有限责任公司 | Rose essential oil skin-moistening lotion and preparation method thereof |
| CN103250865A (en) * | 2013-03-11 | 2013-08-21 | 武汉工业学院 | Preparation method of peanut polypeptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104530197A (en) * | 2015-01-22 | 2015-04-22 | 马淑娟 | Water retention peptide extracted from salix mongolica and application of water retention peptide in cosmetics |
| CN104530197B (en) * | 2015-01-22 | 2017-09-15 | 广州美颂化妆品有限公司 | The water conservation peptide extracted in salix monogolica and its application in cosmetics |
| CN109846746A (en) * | 2019-02-14 | 2019-06-07 | 重庆市洲仨科技发展有限公司 | A kind of compound maintenance polypeptide of natural activity and preparation method thereof |
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