CN109846746A - A kind of compound maintenance polypeptide of natural activity and preparation method thereof - Google Patents
A kind of compound maintenance polypeptide of natural activity and preparation method thereof Download PDFInfo
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- CN109846746A CN109846746A CN201910114364.5A CN201910114364A CN109846746A CN 109846746 A CN109846746 A CN 109846746A CN 201910114364 A CN201910114364 A CN 201910114364A CN 109846746 A CN109846746 A CN 109846746A
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- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to biological product technical fields, more particularly to a kind of compound maintenance polypeptide of natural activity and preparation method thereof, the maintenance polypeptide includes following raw material: 10%~20% sunflower seed extract, 15%~20% peanut seed capsule extract, 1%~5% ginseng root extract, 5%~10% Carrot Roots extract, surplus is deionized water, and wherein sunflower seed extract and peanut seed capsule extract mainly include tripeptides and hexapeptide.A kind of compound maintenance polypeptide of natural activity of the invention, is easy to be absorbed by the skin, and can increase the oxidation resistance of skin, there is epidermal growth factor simultaneously, damaged skin can be allowed to be repaired, improve skin colour, allow skin to radiate vigour again and healthy and beautiful physiological function is presented.
Description
Technical field
The present invention relates to biological product technical field more particularly to a kind of compound maintenance polypeptide of natural activity and its preparation sides
Method.
Background technique
Peptide is the compound that two or more amino acid are connected with peptide bond, and important physiological action is played in human body,
Play physiological function.Active polypeptide is known as active peptide, also known as biologically active peptide or biologically active polypeptide.Active peptide is main
Growth, development, immunological regulation and the metabolism of human body are controlled, it is in a kind of equilibrium state in human body, if active peptide is reduced
Afterwards, important change occurs for the function of human body, and for children, his growth, development becomes slowly, or even stops, under permanent
It goes to be formed dwarf, to adult or the elderly, after lacking active peptide, the immunity of itself will decline, and metabolism is disorderly
Disorderly, endocrine disorder, the generation to give rise to diseases, such as insomnia, lean body mass or edema.Since active peptide also acts on nerve
System, therefore human body will become slow movement, brains is no longer wise, and more important is active peptide reductions, directly causes the person
Each position of body gradually appears comprehensive aging, causes various diseases.
The aging course of human skin and the degree of aging and locating environment, age and the mood of people are closely related.With
The growth at age, the variation and stimulation of outside climatic environment cause the sebaceous glands of skin and sweat gland secretion abnormal, to cause
Small pox.And with advancing age, the sebum of human body, moisture secretion can gradually decrease, the gradually aging of the skin of people, sebum secretion
It reduces, the ability of vitrification moisture can decline, so that skin be made to become more and more dry, the cuticula of skin epidermis is thickened, and produce
Rawhide skin keratinization, dry and cracked, skin surface just become rougher, and resistance can also weaken, and bacterium intrusion causes dermopathic
Occur.Therefore, skin resistance can be enhanced by developing one kind, and the active peptide complexes for repairing impaired skin are necessary.
Summary of the invention
In view of this, be easy to be absorbed by the skin the object of the present invention is to provide a kind of compound maintenance polypeptide of natural activity, and
The oxidation resistance of skin can be increased, while there is epidermal growth factor, damaged skin can be allowed to be repaired, improve skin
Color allows skin to radiate vigour again and healthy and beautiful physiological function is presented.
The present invention solves above-mentioned technical problem by following technological means:
A kind of compound maintenance polypeptide of natural activity, the maintenance polypeptide includes following raw material: 10%~20% sunflower seed
Extract, 15%~20% peanut seed capsule extract, 1%~5% ginseng root extract, 5%~10% Carrot Roots extract,
Surplus is deionized water, and wherein sunflower seed extract and peanut seed capsule extract mainly include tripeptides and hexapeptide.
Further, the maintenance polypeptide includes following raw material: 15% sunflower seed extract, 18% peanut seed capsule extract
Object, 2% ginseng root extract, 8% Carrot Roots extract, surplus is deionized water.
The compound maintenance polypeptide of natural activity of the invention, included in sunflower seed extract and peanut seed capsule extract
Tripeptides and hexapeptide belong to small active peptides, be easy to be absorbed by the skin, and the oxidation resistance of skin can be increased, match simultaneously
Ginseng root extract and Carrot Roots extract are closed, since it is with epidermal growth factor, on the one hand can reduce the external world
Stimulation, increases the defence capability of skin, on the other hand damaged skin can be allowed to be repaired, and improves skin colour, allows skin weight
It newly radiates vigour and healthy and beautiful physiological function is presented.
Furthermore the invention also discloses a kind of above-mentioned preparation method of the compound maintenance polypeptide of natural activity, specifically: take people
After ginseng root extract and carrot extract are mixed, deionized water is added, adds sunflower seed extract, peanut seed capsule
Extract vibrates after mixing, disinfection and sterilization obtains compound maintenance polypeptide.
Further, the sunflower seed extract preparation the following steps are included:
It crushes: after sunflower seed drying is not higher than 10% to water content, 500 meshes is crossed after pulverizing, are obtained
Sunflower seed particle；
Broken wall: it is added in bulking machine after sunflower seed particle and a small amount of deionized water are mixed, is heated to pressure and reaches
It after 0.5MPa, lets out in moment to normal pressure, broken wall sunflower seed particle is obtained after being cooled to room temperature；
It extracts: dispersing obtained broken wall sunflower seed particle in activation deionized water, adjust pH value and be heated to 38-
40 DEG C, heat preservation is added the complex enzyme liquid of 0.02~0.1% broken wall sunflower seed particle mass, is digested in pulsed magnetic field
After 6-8h, 70 DEG C of enzyme deactivations are heated to, then after ultrasonic wave extraction 10-12min, is centrifuged, sunflower seed extracting solution is obtained by filtration.
It is combined by pulverizing with broken wall treatment, the cell wall of sunflower seed can be broken as far as possible, allow it
In nutriment outflow, then the small active peptides such as tripeptides, hexapeptide are decomposed under the action of enzyme, can be in certain journey with this
The concentration of extract is improved on degree, activation deionized water is in addition added during extracting, due to activating deionized water
The property of the small molecule characteristic and energy level with higher that have enables enzyme solution and broken wall sunflower seed particle to mix
More sufficiently, and it can be improved the activity of enzyme, in conjunction with applying pulse magnetic field, pulsed magnetic field action is mild, no fuel factor, therefore not
It will lead to the inactivation of enzyme, simultaneously because joined activation deionized water, under the influence of a magnetic field, be able to maintain that activation deionized water
Activity so that its effect is lasting, the activity function of enzyme can be further increased, and then increase extraction effect.
Further, the pulsed magnetic field includes 3 periods being repeated in, and the pulsewidth of a cycle is 20ms, rises
Along being 2ms, failing edge 2ms, frequency 1.25Hz, magnetic field strength 100mT, the pulsewidth of second period is 15ms, rising edge
For 2ms, failing edge 2ms, frequency 1.25Hz, magnetic field strength 120mT, the pulsewidth in third period is 10ms, rising edge is
2ms, failing edge 2ms, frequency 1.25Hz, magnetic field strength 140mT.
And the pulsed magnetic field of multisection type is used, the quick variation in one side magnetic field can promote the activation in mixed solution to go
Ionized water generates directed movement power, accelerates the movement of hydrone, and hydrone will increase in the process of movement enzyme and broken wall to
The collision of day sunflower seed particle, to accelerate enzyme touching reaction；On the other hand, when reacting the later period, the enzymolysis product in solution is more next
It is more, therefore the progress of enzyme digestion reaction can be inhibited to a certain extent, and by reducing being slightly increased for pulsewidth and length of magnetic field
Mode, enzyme can be stimulated, increase its activity, without inactivating enzyme.
Further, it is described activation deionized water the preparation method comprises the following steps: deionized water is placed in water magnetizing machine, in water velocity
For 0.4~0.6m/s, magnetic field strength carries out magnetization treatment for 24 hours under conditions of being 600~800mT, obtains activation deionized water.
Further, in the broken wall step, the mass ratio of sunflower seed particle and activation deionized water is 5:1.
Further, the complex enzyme liquid includes alkali protease and papain, the alkali protease and pawpaw egg
The mass ratio of white enzyme is 3:1.
Beneficial effects of the present invention:
(1) the compound maintenance polypeptide of a kind of natural activity prepared by the present invention, tripeptides therein and hexapeptide belong to small molecule
Active peptide is easy to be absorbed by the skin, and can increase the oxidation resistance of skin, while having epidermal growth factor, therefore one
Aspect can reduce environmental stimuli, increase the defence capability of skin, on the other hand damaged skin can be allowed to be repaired, and improve skin
Colour of skin pool, allows skin to radiate vigour again and healthy and beautiful physiological function is presented.
(2) present invention extracts Helianthi extract using enzymatic isolation method, is combined using pulverizing with broken wall treatment, will be thin
Cell wall is first crushed, then can be improved the vigor of enzyme under activation deionized water, the synergistic effect of pulsed magnetic field by enzymatic treatment,
Accelerate enzyme touching reaction, so that extraction effect is more preferable.
Specific embodiment
Below with reference to specific embodiment, the present invention is described in detail:
A kind of compound maintenance polypeptide of natural activity of the invention contains the small molecules such as tripeptides, the hexapeptide for being easy to skin absorption
Peptide is easy to be absorbed by the skin, and is free of chemical substance, green, health.
Compound maintenance polypeptide of a kind of natural activity of the invention and preparation method thereof, comprising the following steps:
Embodiment one
It activates the preparation of deionized water: deionized water is placed in water magnetizing machine, be 0.4m/s, magnetic field strength in water velocity
To carry out magnetization treatment for 24 hours under conditions of 800mT, activation deionized water is obtained.
The preparation of sunflower seed extract the following steps are included:
It crushes: after sunflower seed drying is not higher than 10% to water content, 500 meshes is crossed after pulverizing, are obtained
Sunflower seed particle.
Broken wall: mass ratio is mixed and is added in bulking machine later for the sunflower seed particle of 5:1 and a small amount of deionized water, is added
After heat reaches 0.5MPa to pressure, then moment lets out to normal pressure, and broken wall sunflower seed particle is obtained after being cooled to room temperature.
It extracts: dispersing obtained broken wall sunflower seed particle in the activation deionized water of 10 times of mass ratioes, be added
The sodium hydroxide solution of 0.1mol/L adjusts pH value to 8.0, and heating water bath, to 38-40 DEG C, heat preservation adds 0.1% broken wall
The complex enzyme liquid of sunflower seed particle mass after carrying out enzymatic hydrolysis 8h in pulsed magnetic field, is heated to 70 DEG C of enzyme deactivations, is cooled to room temperature
Afterwards, it after carrying out ultrasonic wave extraction 10min under conditions of frequency is 25KHz, power is 200W, is centrifuged, sunflower is obtained by filtration
Seed extracting solution.
The preparation of compound maintenance polypeptide: after taking 2g ginseng root extract and 8g carrot extract to be mixed, 57g is added
Deionized water adds 15g sunflower seed extract, 18g peanut seed capsule extract, vibrates after mixing, disinfection and sterilization is answered
Close maintenance polypeptide.
Embodiment two
It activates the preparation of deionized water: deionized water is placed in water magnetizing machine, be 0.5m/s, magnetic field strength in water velocity
To carry out magnetization treatment for 24 hours under conditions of 700mT, activation deionized water is obtained.
The preparation of sunflower seed extract the following steps are included:
It crushes: after sunflower seed drying is not higher than 10% to water content, 500 meshes is crossed after pulverizing, are obtained
Sunflower seed particle.
Broken wall: mass ratio is mixed and is added in bulking machine later for the sunflower seed particle of 5:1 and a small amount of deionized water, is added
After heat reaches 0.5MPa to pressure, then moment lets out to normal pressure, and broken wall sunflower seed particle is obtained after being cooled to room temperature.
It extracts: dispersing obtained broken wall sunflower seed particle in the activation deionized water of 10 times of mass ratioes, be added
The sodium hydroxide solution of 0.1mol/L adjusts pH value to 8.0, and heating water bath, to 38-40 DEG C, heat preservation adds 0.02% broken wall
The complex enzyme liquid of sunflower seed particle mass after carrying out enzymatic hydrolysis 7h in pulsed magnetic field, is heated to 70 DEG C of enzyme deactivations, is cooled to room temperature
Afterwards, it after carrying out ultrasonic wave extraction 11min under conditions of frequency is 25KHz, power is 200W, is centrifuged, sunflower is obtained by filtration
Seed extracting solution.
The preparation of compound maintenance polypeptide: it after taking 1g ginseng root extract and 10g carrot extract to be mixed, is added
64g deionized water adds 10g sunflower seed extract, 15g peanut seed capsule extract, vibrates after mixing, disinfection and sterilization obtains
Compound maintenance polypeptide.
Embodiment three
It activates the preparation of deionized water: deionized water is placed in water magnetizing machine, be 0.6m/s, magnetic field strength in water velocity
To carry out magnetization treatment for 24 hours under conditions of 600mT, activation deionized water is obtained.
The preparation of sunflower seed extract the following steps are included:
It crushes: after sunflower seed drying is not higher than 10% to water content, 500 meshes is crossed after pulverizing, are obtained
Sunflower seed particle.
Broken wall: mass ratio is mixed and is added in bulking machine later for the sunflower seed particle of 5:1 and a small amount of deionized water, is added
After heat reaches 0.5MPa to pressure, then moment lets out to normal pressure, and broken wall sunflower seed particle is obtained after being cooled to room temperature.
It extracts: dispersing obtained broken wall sunflower seed particle in the activation deionized water of 10 times of mass ratioes, be added
The sodium hydroxide solution of 0.1mol/L adjusts pH value to 8.0, and heating water bath, to 38-40 DEG C, heat preservation adds 0.05% broken wall
The complex enzyme liquid of sunflower seed particle mass after carrying out enzymatic hydrolysis 6h in pulsed magnetic field, is heated to 70 DEG C of enzyme deactivations, is cooled to room temperature
Afterwards, it after carrying out ultrasonic wave extraction 12min under conditions of frequency is 25KHz, power is 200W, is centrifuged, sunflower is obtained by filtration
Seed extracting solution.
The preparation of compound maintenance polypeptide: after taking 5g ginseng root extract and 5g carrot extract to be mixed, 50g is added
Deionized water adds 20g sunflower seed extract, 20g peanut seed capsule extract, vibrates after mixing, disinfection and sterilization is answered
Close maintenance polypeptide.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention.For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways.Here all embodiments can not be exhaustive.It is all to belong to this hair
The obvious changes or variations that bright technical solution is extended out are still in the scope of protection of the present invention.
Claims (8)
1. a kind of compound maintenance polypeptide of natural activity, which is characterized in that the maintenance polypeptide includes following raw material: 10%~20%
Sunflower seed extract, 15%~20% peanut seed capsule extract, 1%~5% ginseng root extract, 5%~10% carrot
Root extract, surplus are deionized water, and wherein sunflower seed extract and peanut seed capsule extract mainly include tripeptides and hexapeptide.
2. the compound maintenance polypeptide of a kind of natural activity according to claim 1, which is characterized in that the maintenance polypeptide includes
Following raw material: 15% sunflower seed extract, 18% peanut seed capsule extract, 2% ginseng root extract, 8% Carrot Roots mention
Object is taken, surplus is deionized water.
3. a kind of preparation method of the compound maintenance polypeptide of natural activity according to claim 2, which is characterized in that the system
Preparation Method specifically: after taking ginseng root extract and carrot extract to be mixed, deionized water is added, adds sunflower
Seed extract, peanut seed capsule extract vibrate after mixing, disinfection and sterilization obtains compound maintenance polypeptide.
4. a kind of preparation method of the compound maintenance polypeptide of natural activity according to claim 3, which is characterized in that it is described to
The preparation of day sunflower seed extract the following steps are included:
It crushes: after sunflower seed drying is not higher than 10% to water content, 500 meshes is crossed after pulverizing, are obtained to day
Sunflower seed particle；
Broken wall: it is added in bulking machine after sunflower seed particle and a small amount of deionized water are mixed, is heated to pressure and reaches
After 0.5MPa, then moment lets out to normal pressure, and broken wall sunflower seed particle is obtained after being cooled to room temperature；
It extracts: dispersing obtained broken wall sunflower seed particle in activation deionized water, adjust pH value and be heated to 38-40
DEG C, heat preservation is added the complex enzyme liquid of 0.02~0.1% broken wall sunflower seed particle mass, enzymatic hydrolysis 6- is carried out in pulsed magnetic field
After 8h, 70 DEG C of enzyme deactivations are heated to, then after ultrasonic wave extraction 10-12min, is centrifuged, sunflower seed extracting solution is obtained by filtration.
5. a kind of preparation method of the compound maintenance polypeptide of natural activity according to claim 4, which is characterized in that the arteries and veins
Rushing magnetic field includes 3 periods being repeated in, and the pulsewidth of a cycle is 20ms, rising edge 2ms, failing edge 2ms, frequency
Rate is 1.25Hz, magnetic field strength 100mT, and the pulsewidth of second period is 15ms, rising edge 2ms, failing edge 2ms, frequency
For 1.25Hz, magnetic field strength 120mT, the pulsewidth in third period is 10ms, rising edge 2ms, failing edge 2ms, frequency are
1.25Hz, magnetic field strength 140mT.
6. a kind of preparation method of the compound maintenance polypeptide of natural activity according to claim 5, which is characterized in that the work
Change deionized water the preparation method comprises the following steps: deionized water is placed in water magnetizing machine, be 0.4~0.6m/s in water velocity, magnetic field is strong
Degree carries out magnetization treatment for 24 hours under conditions of being 600~800mT, obtains activation deionized water.
7. a kind of preparation method of the compound maintenance polypeptide of natural activity according to claim 6, which is characterized in that described broken
In wall step, the mass ratio of sunflower seed particle and deionized water is 5:1.
8. a kind of preparation method of the compound maintenance polypeptide of natural activity according to claim 7, which is characterized in that described multiple
Synthase liquid includes alkali protease and papain, and the mass ratio of the alkali protease and papain is 3:1.
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| CN201910114364.5A CN109846746A (en) | 2019-02-14 | 2019-02-14 | A kind of compound maintenance polypeptide of natural activity and preparation method thereof |
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| CN113170870A (en) * | 2021-05-27 | 2021-07-27 | 绍兴市果哥食品科技有限公司 | Fruit and vegetable crisp chips and production process thereof |
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Application publication date: 20190607 |