CN103505403B - Epidermal growth factor composite liposome body and its preparation method and application - Google Patents
Epidermal growth factor composite liposome body and its preparation method and application Download PDFInfo
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Abstract
The invention belongs to the field of Chinese medicines, disclose a kind of epidermal growth factor composite liposome body and its preparation method and application.This epidermal growth factor composite liposome body is prepared from by following raw material according to the mass fraction: epidermal growth factor 0.1 ~ 5 part, silibinin 5 ~ 30 parts, coix seed oil 5 ~ 30 parts, yeast extract 5 ~ 30 parts, microbial polysaccharide 5 ~ 30 parts, hydrolecithin 30 ~ 200 parts, 10 ~ 80 parts, cholesterol, glycerol 5 ~ 30 parts.Present invention process is simple, safety non-toxic, and little to skin irritation, character is gentle, effect stability.
Description
Technical field
The invention belongs to the field of Chinese medicines, particularly a kind of epidermal growth factor composite liposome body and its preparation method and application.
Background technology
At present, traditional cosmetics, just to the supply of the external nutrition of skin with hide ageing skin, cannot stop the aging of skin.And conventional method manufacture nutrient and active matter cosmetics actual effect little, because nutrient and active matter not easily penetrate into deep skin, normal skin can only absorb some micromolecule and liposoluble substance, nonabsorbable to macro-molecular protein, in cell more difficult to get access, active matter owing to being subject to external condition impact, in use also often early the sixth of the twelve Earthly Branches inactivation.Epidermal growth factor (EGF) is as peptide and protein; to sensitivities such as enzyme, light, pH values; there is the features such as poor stability, easy in inactivation, bioavailability be low; this makes it apply to be very limited; for solving the problem; a lot of cosmetics merely by epidermal growth factor after liposomal encapsulated; form single epidermal growth factor liposome to use; although completely cut off the effect such as enzyme, pH value; certain protection has been had to the activity of epidermal growth factor; but utilization rate is still undesirable, the skin protection skin and complexion improving effect of epidermal growth factor can't be fully played.For these problems; through years of researches; epidermal growth factor is combined with nutrition elite; form a kind of mutual synergism; and make liposome and apply in cosmetics; the effective time of nutrient and active matter is greatly extended. various nutrient and active matter are penetrated in cell and is fully absorbed utilization; cosmetics are made not only to have effect of skin protection, nourishing and protection; the metabolism of cell can also be participated in simultaneously; fundamentally make the smooth high resilience of skin, keep skin natural health.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, primary and foremost purpose of the present invention is to provide a kind of epidermal growth factor composite liposome body.
Another object of the present invention is the preparation method providing a kind of above-mentioned epidermal growth factor composite liposome body.
Another object of the present invention is the application providing above-mentioned epidermal growth factor composite liposome body.
Object of the present invention is achieved through the following technical solutions: a kind of epidermal growth factor composite liposome body, and this epidermal growth factor composite liposome body is prepared from by following raw material according to the mass fraction:
Described epidermal growth factor composite liposome body is more preferably and is prepared from by following raw material according to the mass fraction:
The preparation method of above-mentioned a kind of epidermal growth factor composite liposome body, comprises following operating procedure:
(1) 30 ~ 200 mass parts hydrolecithins, 10 ~ 80 mass parts cholesterol, 5 ~ 30 mass parts silibinin and 5 ~ 30 mass parts coix seed oils are dissolved in dehydrated alcohol join to obtain film material solution; 0.1 ~ 5 mass parts epidermal growth factor, 5 ~ 30 mass parts yeast extracts, 5 ~ 30 mass parts microbial polysaccharides and 5 ~ 30 mass parts glycerol are dissolved in phosphate buffer and join to obtain EGF solution; The consumption of described dehydrated alcohol is the dehydrated alcohol that every 30 ~ 200mg hydrolecithin uses 50ml; The consumption of described phosphate buffer is the phosphate buffer that every 0.1 ~ 5mg epidermal growth factor uses 10ml;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, emulsified 20 ~ 50 minutes of pressure 10 ~ 30Mpa, temperature 30 ~ 60 DEG C of conditions, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 30 ~ 60 DEG C, and separating pressure is 5 ~ 15Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body.
The pH of step (1) described phosphate buffer is 6.8.
The pressure of step (2) described emulsifying is 20Mpa, and temperature is 45 DEG C, and emulsification times is 35 minutes.
Above-mentioned epidermal growth factor composite liposome body is preparing the application in skin care item.
The mass percentage of described epidermal growth factor composite liposome body in skin care item is 0.01 ~ 10%, is more preferably mass percentage 0.05 ~ 5%.
Containing whitening agent, wetting agent, antioxidant, surfactant, alcohol compound or water in described skin care item.
Described skin care item are solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or spray.
The pharmacologic effect of various raw material is as follows:
Epidermal growth factor (EGF) is the somatomedin found the earliest, exchanges ganglion cell's growth, propagation and differentiation and plays an important role.Be widely used in the treatment of various wound clinically, especially the treatment of skin trauma, obtain good curative effect.Epidermal growth factor EGF enters cell division cycle by irritating epidermis cell (comprising epithelial cell, various Interstitial cell that Various Tissues is originated) with the combination of EGF receptor high-affinity, some critical function gene activations, expression, secretion biological activity protein etc. in active cell.Collagen fiber are impelled to be the linear alignment, the growth of epidermis cell fast regular timely flap coverage, start the process of wound repair, after obviously accelerating beauty treatment, cosmetology and the wound healing such as other skin trauma, and keep wound surface flat smooth, reduce abnormal pigmentary deposit on the skin trace and pigmentation.Modern study finds, epidermal growth factor can irritate the various cells of ectoderm and endodermal origin as corneal epithelium and endotheliocyte, epidermis, skin corium cell (as fibroblast), mammary gland alveolus and Interstitial cell etc., make its proliferation apoptosis, accelerate metabolism, reach the effect of skin care.The biosynthesis of various protein can be promoted simultaneously, promote the macromolecular synthesis in extracellular (as hyaluronic acid, elastic fiber albumen etc.), increase moisture content of skin, and then increase skin elasticity, Glycerin.Promote that cytotrophy material is initiatively transported in cell from extracellular, increase intracellular nutrition, promote the functional moleculars such as skin corium emiocytosis rubber polymer fibril, polysaccharide, glycoprotein, make that subcutaneous dermal tissue is full, muscle fiber marshalling tight, thus reduce and smooth away wrinkles.Stronger facilitation is had to the propagation of epidermis cell, make youthening of epidermis cell, make the less accumulation such as residual having pigment and dead cell in epidermal tissue, cutaneous manifestations is gone out and tenderly to have no time in vain, eliminate the performance of the abnormal skin such as mottle and pigmentation, effectively can contain the recurrence of mottle.
Silibinin is a kind of flavanolignan compounds extracting gained in the seed coat of the medicinal plants of Compositae Herba Silybi mariani seed, can promote regeneration and the reparation of cell, have the effect of stabilized cell and intracellular biological film.As powerful antioxidant, there is antioxidation and antidotal effect, the free radical in human body can be removed, slow down aging.
Semen Coicis ester, Semen Coicis element, unsaturated fatty acid, protein, vitamin etc. are rich in coix seed oil, heat clearing away draining can alleviate skin fatigue, can effectively stop undesirable cell to grow, maintenance cell maintains cell elasticity, promote the metabolism of skin, make skin whitening and high resilience.Also be Chinese distinctive base oil, skin is strong to the absorbing power of coix seed oil simultaneously, has the effect of mediation and effect concurrently.
Yeast extract adopts the natural material coming from yeast to refine and forms, that yeast will the wherein extracting such as protein, nucleic acid, vitamin after breaking cellular wall, then through the material being rich in the active skull cap components such as micromolecular aminoacid, peptide, nucleotide, vitamin of biological enzymolysis.Wherein containing the abundant bioactive ingredients such as aminoacid, peptide, nucleic acid (nucleotide), there is nourishes rough skin, anti-ageing function of waiting for a long time.
Microbial polysaccharide is a kind of active polysaccharide deriving from microorganism; polysaccharide is as human body skin skin corium important composition composition; in skin metabolism process, there is outstanding regulating action; be applied in cosmetics; moisturizing can be produced, stablize, improve the colour of skin, defying age and antibacterial; protection blood vessel, promotes the effects such as epithelial cell proliferation and beautifying skin, has the advantages such as pleiotropy, avirulence, wide material sources, natural green.
Principle of the present invention is:
The skin protection skin and complexion improving effect of epidermal growth factor depends on the biological activity of its uniqueness; make full use of its physiological property; make it play a role to greatest extent in skin metabolism process, it will be protected fully active on the one hand, also will protect the abundant moisture of skin on the other hand.In epidermal growth factor complex, silibinin and coix seed oil, except having the nutritional labeling effect of itself, owing to having strong non-oxidizability and anti-ultraviolet ability, play a very good protection to the activity of epidermal growth factor.Study simultaneously and also find that the Moisture factor such as epidermal growth factor EGF and peptides has excellent positive cooperativity; therefore yeast extract and microbial polysaccharide is added in preparation containing in epidermal growth factor complex; well play protection EGF biological activity and the sufficient moisture of skin, thus improve the oozing property thoroughly of EGF and the effect of favourable skin absorption EGF.The good physiological property had according to epidermal growth factor complex and the feature of liposome, epidermal growth factor complex is made a kind of Liposome Cosmeatics, because the phospholipid bilayer film of liposome and cell have identical basic structure, both have very strong affinity, and liposome and the epidermal growth factor complex that comprises thereof are easily entered in cell.After liposome enters deep skin. what have enters in cell, and what have stops at iuntercellular.Due to phospholipid bilayer film around, epidermal growth factor complex in liposome can infiltrate slowly through liposome membrane, thus the long period plays a role. can maintain vigour the long period, improve the bioavailability of epidermal growth factor complex greatly, thus cell is repaired and adjusts, comprehensive improvement and renewal metabolic process, promote the growth of Skin Cell, regulate the balance of pigment in cell, Reasonable adjustment skin texture, make Skin Cell rejuvenation, reach moist, crease-resistant, whitening, skin care, the objects such as anti-acne speckle, the smooth high resilience of skin is fundamentally made by the metabolism of cell own, keep skin natural health.
Epidermal growth factor composite liposome body of the present invention adopts the preparation of CO 2 supercritical technology.Carbon dioxide has the dual characteristics of gas-liquid two-phase concurrently in the supercritical state, and the carbon dioxide being in different phase has different density, thus has different solvent strengths.Utilize supercritical carbon dioxide to dissolve, expand, the character of precipitating, solution containing EGF, yeast extract and microbial polysaccharide active component is mixed with the film material solution containing silibinin and coix seed oil nutritional labeling, pass into supercritical carbon dioxide under certain condition, make carbon dioxide permeability and swelling phospholipid bilayer, after keeping certain hour, then quick release pressure, isolates epidermal growth factor composite liposome body.Traditional multiple method preparing liposome, as reverse evaporation, film dispersion method, ultrasonic dispersion, organic solvent injection method etc., these methods all must add harmful organic solvent in the process of preparation, and supercritical carbon dioxide be a kind of nontoxic, inertia, do not fire, the reaction medium of environmental protection cheap and easy to get, supercritical carbon dioxide is adopted to replace organic solvent preparation table skin growth factor composite liposome body, its major advantage has: economize on resources, do not have organic solvent residual, operation is simple and feasible, improves the utilization rate of EGF.
The present invention has following advantage and beneficial effect relative to prior art: present invention process is simple, safety non-toxic, and little to skin irritation, character is gentle, effect stability.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) 30mg hydrolecithin, 10mg cholesterol, 5mg silibinin and 5mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 0.1mg epidermal growth factor, 5mg yeast extract, 5mg microbial polysaccharide and 5mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 10Mpa at pressure, temperature to reach in 30 DEG C emulsifying 20 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 30 DEG C, and separating pressure is 5Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body I.
Embodiment 2
(1) 50mg hydrolecithin, 15mg cholesterol, 10mg silibinin and 10mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 0.5mg epidermal growth factor, 8mg yeast extract, 8mg microbial polysaccharide and 10mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 15Mpa at pressure, temperature to reach in 35 DEG C emulsifying 25 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 35 DEG C, and separating pressure is 6Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body II.
Embodiment 3
(1) 80mg hydrolecithin, 25mg cholesterol, 12mg silibinin and 20mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 1mg epidermal growth factor, 10mg yeast extract, 10mg microbial polysaccharide and 15mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 15Mpa at pressure, temperature to reach in 40 DEG C emulsifying 30 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 40 DEG C, and separating pressure is 8Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body III.
Embodiment 4
(1) 100mg hydrolecithin, 35mg cholesterol, 15mg silibinin and 20mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 2mg epidermal growth factor, 15mg yeast extract, 15mg microbial polysaccharide and 15mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 20Mpa at pressure, temperature to reach in 45 DEG C emulsifying 35 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 40 DEG C, and separating pressure is 10Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body IV.
Embodiment 5
(1) 150mg hydrolecithin, 50mg cholesterol, 20mg silibinin and 25mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 3mg epidermal growth factor, 20mg yeast extract, 20mg microbial polysaccharide and 20mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 20Mpa at pressure, temperature to reach in 50 DEG C emulsifying 40 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 50 DEG C, and separating pressure is 10Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body V.
Embodiment 6
(1) 180mg hydrolecithin, 60mg cholesterol, 25mg silibinin and 25mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 4mg epidermal growth factor, 25mg yeast extract, 25mg microbial polysaccharide and 25mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 25Mpa at pressure, temperature to reach in 55 DEG C emulsifying 45 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 55 DEG C, and separating pressure is 12Mpa; ) collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body VI
Embodiment 7
(1) 200mg hydrolecithin, 80mg cholesterol, 30mg silibinin and 30mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 5mg epidermal growth factor, 30mg yeast extract, 30mg microbial polysaccharide and 30mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 30Mpa at pressure, temperature to reach in 60 DEG C emulsifying 50 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 60 DEG C, and separating pressure is 15Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body VII.
Comparative example 1
(1) 100mg hydrolecithin, 35mg cholesterol, 15mg silibinin are dissolved in the dehydrated alcohol of 50ml and join to obtain film material solution; 2mg epidermal growth factor, 15mg yeast extract, 15mg microbial polysaccharide and 15mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 20Mpa at pressure, temperature to reach in 45 DEG C emulsifying 35 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 40 DEG C, and separating pressure is 10Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body A.
Comparative example 2
(1) 100mg hydrolecithin, 35mg cholesterol, 20mg coix seed oil are dissolved in the dehydrated alcohol of 50ml and join to obtain film material solution; 2mg epidermal growth factor, 15mg yeast extract, 15mg microbial polysaccharide and 15mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 20Mpa at pressure, temperature to reach in 45 DEG C emulsifying 35 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 40 DEG C, and separating pressure is 10Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body B.
Comparative example 3
(1) 100mg hydrolecithin, 35mg cholesterol, 15mg silibinin and 20mg coix seed oil are dissolved in the dehydrated alcohol of 50ml join to obtain film material solution; 2mg epidermal growth factor and 15mg glycerol are dissolved in 10ml phosphate buffer (PH6.8) and join to obtain EGF solution;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, reach 20Mpa at pressure, temperature to reach in 45 DEG C emulsifying 35 minutes, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 40 DEG C, and separating pressure is 10Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body C.
By to the above size of embodiment 1-7 gained epidermal growth factor composite liposome body and the mensuration of envelop rate, the results are shown in Table 1.
Table 1 epidermal growth factor composite liposome body size and envelop rate
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 | |
| Mean diameter | 106.2nm | 108.6nm | 109.1nm | 100.2nm | 100.8nm | 105.8nm | 110.8nm |
| Envelop rate | 40% | 42% | 44% | 42% | 40% | 38% | 38% |
The mode of appearance of epidermal growth factor composite liposome body of the present invention is the standard reflecting liposome quality intuitively, above-mentioned experimental result shows, the epidermal growth factor composite liposome body particle shape adopting CO 2 supercritical technology to prepare is spherical, flush edge is smooth, particle diameter is normal distribution, particle shape no significant difference between each experimental example, shows its preparation method reasonably stability.Supercritical methanol technology is adopted to prepare in the process of liposome, the emulsifying of phospholipid is very important step, control the quality critical that suitable emulsification condition (pressure, temperature and time) is liposomal particle size size and envelop rate, now carbon dioxide forms supercritical fluid, the ability of dissolving phospholipid is similar with non-polar organic solvent, the phospholipid solution be dissolved in supercritical carbon dioxide and aqueous phase are uniformly mixed to form Emulsion, just can obtain uniform liposome solutions after decompression removing carbon dioxide, also solve the problem of organic solvent residual simultaneously.Best emulsification condition is pressure 20Mpa, temperature 45 C, 35 minutes time.
Application implementation example
(1) containing the preparation of emollient cream of epidermal growth factor composite liposome body
Get above-described embodiment and comparative example gained epidermal growth factor composite liposome body is mixed with emollient cream respectively, formula following (by mass percentage):
Processing technology containing emollient cream of epidermal growth factor composite liposome body is as follows:
(1) in oil phase pot, add the Jojoba oil in oil phase A, Butyrospermum parkii fruit fat, Cyclomethicone, polydimethylsiloxane, glyceryl stearate, cetearyl alcohol, sad/triglyceride, PEG-20 methyl sesquistearate, methyl sesquistearate and four-(dibutyl alkyl hydrocinnamic acid) pentaerythritol ester, be heated to 85 DEG C while stirring;
(2) in aqueous phase pot, add deionized water, carbomer, pantothenylol, glycerol and the EDETATE SODIUM in aqueous phase B, be heated to 85 DEG C while stirring;
(3) to emulsifying pot to carry out after evacuation oil pumping respectively mutually and aqueous phase equal to the homogeneous stirring of emulsifying pot;
(4) after temperature is down to 65 DEG C, adds C phase triethanolamine homogenizing again, adds (daily) essence in D phase after being cooled to 45 DEG C, Methylisothiazolinone, under stirring, add E phase epidermal growth factor composite liposome body;
(5) stop stirring after temperature is down to 36 DEG C, discharging, obtains epidermal growth factor composite liposome body emollient cream.
Emollient cream prepared by embodiment 1-7 respectively epidermal growth factor composite liposome body I, II, III, IV, V, VI, VII obtained emollient cream I, II containing epidermal growth factor composite liposome body, III, IV, V, VI, VII according to the method described above.
Epidermal growth factor composite liposome body A, B, C obtained contrast emollient cream A, B, C containing epidermal growth factor composite liposome body according to the method described above that contrast emollient cream is prepared by comparative example 1-3 respectively.
Above-mentioned emollient cream prepared is carried out clinic trial observation.
The object of observation: 25 ~ 45 years old age women.Choosing face has women 100 example of the problem skin such as mottle in various degree, wrinkle, lax, acne, damaging skin.Be divided into five groups, often organize 20 examples
Observational technique: before sleep every night after cleaning skin, coat above-mentioned emollient cream.Continuous use 28 days is a course for the treatment of.
Criterion of therapeutical effect: (1) is effective: speckle pox wrinkle reduces, elasticity strengthens, damaging skin repair reaches more than 90%, and skin has obvious pale moist smooth feeling; (2) effective: speckle pox wrinkle reduces, elasticity strengthens, damaging skin repair reaches more than 50%, skin is more pale moist smooth; (3) invalid: unchanged before and after treatment.Result such as table 2 shows:
Table 2 emollient cream effect comparison table
| Effective | Effectively | Invalid | Total effective rate | |
| Emollient cream I | 15 | 3 | 2 | 90% |
| Emollient cream II | 15 | 2 | 3 | 85% |
| Emollient cream III | 13 | 5 | 2 | 90% |
| Emollient cream IV | 18 | 1 | 1 | 95% |
| Emollient cream V | 16 | 3 | 1 | 95% |
| Emollient cream VI | 17 | 1 | 2 | 90% |
| Emollient cream VII | 14 | 4 | 2 | 90% |
| Contrast emollient cream A | 5 | 10 | 5 | 75% |
| Contrast emollient cream B | 3 | 13 | 4 | 80% |
| Contrast emollient cream C | 3 | 10 | 7 | 65% |
(2) containing the preparation of the milky lotion of epidermal growth factor composite liposome body
Get above-described embodiment and comparative example gained epidermal growth factor composite liposome body is mixed with milky lotion respectively, formula following (by mass percentage):
Processing technology containing the milky lotion of epidermal growth factor composite liposome body is as follows:
After raw material in A phase and B phase mixes respectively, be heated to 85 DEG C, C is added to B phase, stir, A is added in BC mixed phase, after homogenizing, be cooled to 45 DEG C and add D phase, under stirring, add E phase epidermal growth factor composite liposome body, 36 DEG C stop stirring discharging, obtain epidermal growth factor composite liposome body milky lotion.
Milky lotion is obtained containing epidermal growth factor composite liposome body milky lotion I, II, III, IV, V, VI, VII according to the method described above by epidermal growth factor composite liposome body I, II, III, IV, V, VI, VII respectively.
Contrast emollient cream is respectively by epidermal growth factor composite liposome body A, B, C obtained emollient cream A, B, C containing epidermal growth factor composite liposome body according to the method described above.
The above-mentioned milky lotion containing epidermal growth factor composite liposome body prepared is carried out clinic trial observation.
The object of observation: 25 ~ 45 years old age women.Choosing face has the women 100 of pigementation in various degree, the problem skin such as dark yellow, matt, dry routine.Be divided into five groups, often organize 20 examples
Observational technique: before sleep every night after cleaning skin, coats above-mentioned containing epidermal growth factor composite liposome body skin care milk.Continuous use 28 days is a course for the treatment of.
Criterion of therapeutical effect: (1) is effective: dark yellow the disappearing of pigementation reaches more than 90%, skin has obvious whitening to moisten; (2) effective: pigementation is dark yellow disappears more than 50%, skin comparatively whitening moistens; (3) invalid: unchanged before and after treatment.Result such as table 3 shows:
The effect comparison table of table 3 milky lotion
| Effective | Effectively | Invalid | Total effective rate | |
| Emollient cream I | 15 | 3 | 2 | 90% |
| Emollient cream II | 16 | 1 | 3 | 85% |
| Emollient cream III | 18 | 1 | 1 | 95% |
| Emollient cream IV | 17 | 2 | 1 | 95% |
| Emollient cream V | 16 | 2 | 2 | 90% |
| Emollient cream VI | 15 | 2 | 3 | 85% |
| Emollient cream VII | 17 | 1 | 2 | 90% |
| Contrast emollient cream A | 4 | 11 | 5 | 75% |
| Contrast emollient cream B | 5 | 10 | 5 | 75% |
| Contrast emollient cream C | 5 | 7 | 8 | 60% |
Above-mentioned experimental result shows; epidermal growth factor composite liposome body of the present invention makes an addition in cosmetics as one containing multi-functional strong cellular metabolism promotive factor; remarkable to the nursing efficacy of skin; have smoothly to moisten, whitening skin and preserving moisture, defying age, the damaging skin of reparation, the effect such as speckle dispelling pox, for skin provides omnibearing protection.Generally, the addition of epidermal growth factor composite liposome body in cosmetics is 0.5%, to the nursing of some specific skin as defying age, to reduce microgroove, repair the addition of the epidermal growth factor composite liposome bodies such as damaging skin in cosmetics be 1%.Be with a wide range of applications and market prospect.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. an epidermal growth factor composite liposome body, is characterized in that: this epidermal growth factor composite liposome body is prepared from by following raw material according to the mass fraction:
The preparation method of described epidermal growth factor composite liposome body, comprises following operating procedure:
(1) 30 ~ 200 mass parts hydrolecithins, 10 ~ 80 mass parts cholesterol, 5 ~ 30 mass parts silibinin and 5 ~ 30 mass parts coix seed oils are dissolved in dehydrated alcohol join to obtain film material solution; 0.1 ~ 5 mass parts epidermal growth factor, 5 ~ 30 mass parts yeast extracts, 5 ~ 30 mass parts microbial polysaccharides and 5 ~ 30 mass parts glycerol are dissolved in phosphate buffer and join to obtain EGF solution; The consumption of described dehydrated alcohol is the dehydrated alcohol that every 30 ~ 200mg hydrolecithin uses 50ml; The consumption of described phosphate buffer is the phosphate buffer that every 0.1 ~ 5mg epidermal growth factor uses 10ml;
(2) above-mentioned film material solution and EGF solution are loaded in supercritical autoclave, airtight supercritical autoclave, passes into CO through CO 2 high pressure pump
2, emulsified 20 ~ 50 minutes of pressure 10 ~ 30Mpa, temperature 30 ~ 60 DEG C of conditions, CO
2flow velocity is 15L/h; Decompression separation goes out emulsification product, and separation temperature is 30 ~ 60 DEG C, and separating pressure is 5 ~ 15Mpa; Collect the emulsification product after being separated, obtain epidermal growth factor composite liposome body.
2. a kind of epidermal growth factor composite liposome body according to claim 1, is characterized in that: this epidermal growth factor composite liposome body is prepared from by following raw material according to the mass fraction:
3. a kind of epidermal growth factor composite liposome body according to claim 1, is characterized in that: the pH of step (1) described phosphate buffer is 6.8.
4. a kind of epidermal growth factor composite liposome body according to claim 1, is characterized in that: the pressure of step (2) described emulsifying is 20Mpa, and temperature is 45 DEG C, and emulsification times is 35 minutes.
5. a kind of epidermal growth factor composite liposome body according to claim 1 is preparing the application in skin care item.
6. application according to claim 5, is characterized in that: the mass percentage of described epidermal growth factor composite liposome body in skin care item is 0.5 ~ 1%.
7. application according to claim 5, is characterized in that: containing whitening agent, wetting agent, antioxidant, surfactant, alcohol compound or water in described skin care item.
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| CN104382833B (en) * | 2014-10-28 | 2015-11-18 | 广东碧嫄化妆品有限公司 | Plant protein peptide stock solution and its preparation method and application |
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| CN106038464A (en) * | 2016-07-11 | 2016-10-26 | 佛山杰致信息科技有限公司 | Composite anti-wrinkle skin care product and preparation method thereof |
| CN106074190B (en) * | 2016-07-29 | 2019-02-19 | 广东科玮生物技术股份有限公司 | A kind of anti-aging visage block cream and preparation method thereof |
| CN106377468A (en) * | 2016-11-30 | 2017-02-08 | 上海儿童营养中心有限公司 | Composition for preventing and treating diaper dermatitis and lipidosome and diaper ointment containing composition |
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