CN112137895A - Eye mask containing tremella polysaccharide and preparation method thereof - Google Patents
Eye mask containing tremella polysaccharide and preparation method thereof Download PDFInfo
- Publication number
- CN112137895A CN112137895A CN202011185808.3A CN202011185808A CN112137895A CN 112137895 A CN112137895 A CN 112137895A CN 202011185808 A CN202011185808 A CN 202011185808A CN 112137895 A CN112137895 A CN 112137895A
- Authority
- CN
- China
- Prior art keywords
- tremella polysaccharide
- extract
- eye mask
- eye
- tremella
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0208—Tissues; Wipes; Patches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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Abstract
The invention discloses an eye mask containing tremella polysaccharide and a preparation method thereof. The compound of the tremella polysaccharide and the sclerotium rolfsii gum can obviously improve the wrinkle removing effect of eyes, and can also greatly improve the mechanical property of the eye mask, so that the eye mask is more conformable, not easy to break, good in forming effect and convenient to use. The eye mask can be added with other effective components according to the special characteristics of the eye skin, and can tighten the eye skin, effectively remove wrinkles, lock the eye water, prevent the water loss, enhance the resistance of the eye skin and delay aging by matching with the other effective components.
Description
Technical Field
The invention relates to an eye mask, in particular to a gel-like eye mask patch which does not use eye mask cloth or eye mask paper, has multiple effects and good mechanical property and a preparation method thereof, and belongs to the technical field of eye masks.
Background
Researches show that modern young people stay up for a long time, the emotion fluctuation is large, eyes are extremely easy to fatigue, blood flow speed of blood vessels of skin of the eyes is too low, stagnation is formed, and fishtail lines and canthus lines are easy to appear; and because the oxygen supply of the tissues is insufficient, the metabolic waste in the blood vessels is accumulated excessively, so that the pigmentation of the eyes is caused, and a black eye is easily formed. Natural growth factors are also not negligible except for human factors, and subcutaneous fat around the eyes becomes thinner with age, so wrinkles and dark circles around the corners of the eyes are aggravated.
Compared with the skin at other parts of the face, the skin around the eyes is particularly soft and has a plurality of folds and larger surface area, so that the moisture evaporation speed of the skin around the eyes is higher; meanwhile, sweat glands and sebaceous glands of skin around eyes are less distributed, and the skin is easy to dry and lack of water. In addition, the aging of human body is the inevitable rule of life growth, wherein the change of skin is most obvious, and after the age of 25 years, the skin around eyes of general women begins to become worse, so that the problems of black eye circles, fishtail lines, eye bags, granulation, edema and the like occur. Therefore, the prevention and care of the eye skin is very important.
At present, cosmetics in the market are various, and cosmetics for caring eye skin comprise eye cream, eye essence, eye mask and the like. The common non-woven fabric eye mask is thick and heavy, is easy to bubble, can fall off when moving and has poor fitting property. The membrane cloth material contains fiber, skin itch can be caused due to static electricity, and meanwhile, most essence liquid is left outside and cannot fully exert the efficacy. The gel eye mask can well improve the problems, but has the defects that some gel eye masks have a mud rubbing phenomenon after being used, some gel eye masks have poor plasticity, and the gel eye masks cannot be suitable for all people according to different eye shapes of people. Compared with the non-woven fabric eye mask, the gel eye mask is not moist enough when being used, and cannot simultaneously play a role in moisturizing, wrinkle removal, dark eye removal and the like.
Therefore, there is a need to develop an eye care product having moisturizing, anti-aging, wrinkle-removing and refreshing functions simultaneously to solve various eye problems caused by growth factors and human factors simultaneously.
Disclosure of Invention
The invention aims to provide an eye mask containing tremella polysaccharide, which contains active ingredients of tremella polysaccharide, wherein the tremella polysaccharide has a good wrinkle removing effect, can play a role in synergy by matching with sclerotium rolfsii gum, and can improve the mechanical property of the eye mask by matching the tremella polysaccharide with the sclerotium rolfsii gum, so that the eye mask is more convenient to prepare and use.
The invention selects tremella polysaccharide as a main anti-wrinkle component, the tremella polysaccharide is an acidic heteropolysaccharide, the main chain structure is mannan connected by alpha- (1 → 3) glycosidic bond, and the branched chain is tremella heteroglycan composed of glucuronic acid and xylose. The tremella polysaccharide can absorb a large amount of moisture, has the same high moisture retention property no matter under the condition of low humidity or high humidity, is the only one million-level molecular weight natural moisture retention raw material at present, has excellent moisture retention and water locking capacity, and can repair epidermis, increase the moisture content of the epidermis, improve the conductivity integral value of the skin, enhance the skin protection barrier and prevent the moisture from evaporating from the skin. The tremella polysaccharide also has certain capacity of eliminating hydroxyl free radicals, can prevent skin aging by inhibiting peroxidation of cell lipid, can promote growth of elastic cells, reduce damage of UV to the skin, activate epidermal cells, repair photodamaged skin, accelerate skin regeneration, remove wrinkles and wrinkles, and can be applied to cosmetics as an anti-aging and anti-oxidation component. In addition, the tremella polysaccharide has good lubricity and film forming property, can form a layer of uniform film on the surface of skin, and plays roles in moisturizing, lubricating and eliminating static electricity.
The sclerotium rolfsii gum backbone is composed of beta-1, 3-D-glucopyranose, with a pendant beta-1, 6-D-glucopyranose unit group every third glucose unit. Research shows that the sclerotium rolfsii gum has excellent moisturizing, anti-allergic and skin repairing functions. The sclerotium rolfsii gum is used as a pure natural biological polymer humectant, has the functions of moisturizing, repairing, diminishing inflammation, resisting oxidation and the like, and has stable property, good water solubility and long-acting moisturizing.
Aiming at the purpose of providing a gel eye mask paste without using a substrate such as mask cloth or mask paper, experiments show that tremella polysaccharide and sclerotium rolfsii gum can be used as effective components of an eye mask and can improve the mechanical property of the eye mask paste, so that the eye mask paste is more conformable, less prone to fracture, simpler in forming and better in forming effect. The specific technical scheme of the invention is as follows:
an eye mask containing the Tremella polysaccharide comprises the following effective components in percentage by weight: 0.15-0.4% of tremella polysaccharide and 0.3-1.2% of sclerotium rolfsii gum. The mass ratio of the tremella polysaccharide to the sclerotium rolfsii gum is within the range of 1: 0.75-8. Under the content and the mass ratio, the sclerotium rolfsii gum has obvious synergistic effect on the anti-wrinkle effect of the tremella polysaccharide, and the obtained eye mask has better adhesive force and tensile strength, better fit with the skin and difficult fracture.
Further, the molecular weight range of the tremella polysaccharide can be selected within the usual range, such as 0.8 × 106～4.9×106Da，Preferably 3.5X 106～4.5×106Da. When the preferable molecular weight is adopted, the anti-wrinkle performance and the mechanical performance of the eye mask are better.
Furthermore, the tremella polysaccharide obtained by various preparation methods reported in the prior art can be selected, and the tremella polysaccharide obtained by Sevage extraction method, enzymolysis method, chemical method, biological fermentation method and other methods can be used in the invention. The Sevage extraction method, the enzymolysis method and the chemical method are complex in process, toxic reagents can be introduced in the preparation process, the potential safety hazard of the tremella polysaccharide is increased, the biological fermentation method is simple in process and low in cost, the toxic reagents which are harmful to skin can not be introduced, the activity of the tremella polysaccharide is kept to the maximum extent, and therefore the tremella polysaccharide obtained by the biological fermentation method is preferably used as the raw material.
In a specific embodiment of the present invention, the tremella polysaccharides are obtained from a tremella spore fermentation extract obtained by submerged fermentation of tremella spore liquid, and the main component of the tremella polysaccharides is tremella polysaccharides, which can be prepared by the method described in patent CN200410072865.5, for example. When the tremella spore fermentation extract is used, the dosage of the extract is measured according to the quantity of tremella polysaccharide in the extract.
Furthermore, the tremella polysaccharide and the sclerotium rolfsii gum mainly play a role in resisting wrinkles, in order to enrich the efficacy of the eye mask and enable the eye mask to be capable of solving various eye problems caused by different factors, other effective components with different efficacies can be added into the eye mask, for example, a moisturizing component, an anti-aging component, a whitening component, a freckle removing component, a bacteriostatic component and the like can be added, and the components can be one or more, such as a chlorella extract, a cactus extract, a centella extract, a golden yellow chamomile extract, sodium hyaluronate, trehalose and the like. The chlorella extract mainly contains chlorella growth factor which contains abundant nucleic acid and nucleoprotein, and has effects of repairing human body cell, scavenging free radicals, and resisting aging. The radix et caulis Opuntiae Dillenii extract has effects of promoting skin growth factor generation, activating skin, resisting aging, and resisting bacteria. Centella asiatica extract is a fully water-soluble natural active ingredient, has anti-aging and anti-inflammatory effects, can be used as a supplement for treating dry and sensitive skin, and is widely applied to a formula of cosmetics for repairing scars, removing acnes and preserving moisture. The golden yellow chamomile extract is from a flower-like head of a chrysanthemum belonging to a Compositae plant, contains various effective components such as volatile oil, choline, stachydrine, amino acid, flavonoid, chrysanthemum lactone, chrysanthenone, chrysanthenol and the like, can treat dermatitis, has an inhibiting effect on induced allergy of skin, can enhance the resistance of capillary vessels, inhibit capillary permeability, has an anti-inflammatory effect, can eliminate free radicals, has oxidation resistance and an aging relieving effect, has a certain inhibiting effect on melanocyte, and has certain whitening and freckle removing effects. Sodium hyaluronate is a well-known natural moisturizing factor, has the thousand-fold water locking capacity, is a polysaccharide compound contained in a human body, and has compatibility and safety which are not possessed by other high molecular compounds. The moisture level of the skin is closely related to the content of hyaluronic acid, and the content of hyaluronic acid in the skin is reduced along with the increase of the age, so that the water retention function of the skin is weakened, wrinkles are generated, and the sodium hyaluronate has certain wrinkle resistance. In addition, sodium hyaluronate also has the effects of repairing and preventing skin injury, lubricating property, film-forming property, thickening property and the like. The trehalose has stable property and is not easy to be damaged, and the latest research shows that the exogenous trehalose also has good non-specific protection effect on organisms and biomacromolecules. In addition, the sodium hyaluronate and the trehalose are applied in a combined manner, have complementary and synergistic effects, have biological fresh-keeping and intelligent moisturizing effects, and can keep tissues, skins and cosmetics fresh all the time.
Furthermore, the content of the other effective components of the tremella polysaccharide and the sclerotium rolfsii gum in the eye mask can be adjusted and selected according to different effects to be achieved by the eye mask and the general dosage of the effective components in the cosmetics.
In a specific embodiment of the present invention, the chlorella extract is contained in an amount of 0.01 to 0.5 wt%.
In one embodiment of the present invention, the content of the cactus extract is 0.01 to 0.5 wt%.
In one embodiment of the present invention, the content of centella asiatica extract is 0.3 to 0.8 wt%.
In a specific embodiment of the present invention, the content of the golden chamomile extract is 0.01 to 0.5 wt%.
In one embodiment of the present invention, the content of the sodium hyaluronate is 0.1 to 0.8 wt%.
In one embodiment of the present invention, the trehalose is present in an amount of 1 to 4 wt%.
Further, in order to form the eye mask into a desired shape or meet the performance requirements of various dosage forms, it is inevitable to add some auxiliary ingredients to the eye mask, and the auxiliary ingredients may be at least one of a humectant, a thickener, a preservative, water, and the like. The ingredients and amounts of these adjuvants can be selected from those reported in the prior art without difficulty for the person skilled in the art.
In one embodiment of the present invention, the humectant is selected from: one or more of propylene glycol, glycerin, butanediol and allantoin, wherein the content of the humectant is 8.2-26.4 wt%. The propylene glycol is a viscous and slightly sweet liquid, is mutually soluble with water, ethanol and various solvents, and has the effects of moisture absorption, lubrication and certain sterilization. Glycerin can absorb moisture in the air to moisturize the skin and also can lock the moisture in the skin to retain the moisture in the stratum corneum, so that the body shadow of glycerin can be seen in various cosmetics. Butanediol is a micromolecular moisturizing component, is often used as a moisturizing agent in cosmetics, and also has a certain antibacterial effect. Allantoin is a derivative of uric acid, is a very common humectant and anti-allergy agent, and is cheap and good. Allantoin can promote the growth of cell tissue, influence the rapid granulation of epidermis, accelerate the renewal process, and repair skin.
In a specific embodiment of the invention, the thickener is selected from carbomer and/or glucomannan, and the content of the thickener is 3.0-7.5 wt%.
In a specific embodiment of the present invention, the preservative is selected from dipotassium glycyrrhizinate and/or methylparaben, and the content of the preservative is 0.02-0.4 wt%. The dipotassium glycyrrhizinate is prepared by extracting effective components from liquorice, can penetrate into the skin, keeps high activity, whitens skin, resists oxidation efficiently, effectively inhibits the activity of various enzymes in the process of melanin generation, and has the effects of preventing rough skin, resisting inflammation and resisting bacteria. Methyl hydroxybenzoate can inhibit growth of various microorganisms, and compared with traditional antiseptic products (such as sodium benzoate), the product has the advantages of low use concentration, broad spectrum, high efficiency, safety, economy, long service life, etc., and can be widely used for antiseptic of cosmetics, medicines, foods and other industrial products.
Preferably, the invention also provides a wrinkle-removing eye mask which has the effects of moisturizing, resisting aging and refreshing, can solve the symptoms of wrinkles, dryness, aging, wrinkles and the like of eye skin, and has the following formula (wt%): polyol: 8.2-26.4%, thickening agent: 3.0 to 7.5%, allantoin 0.01 to 0.4%, dipotassium glycyrrhizinate 0.01 to 0.2%, methylparaben: 0.01-0.2%, 0.01-0.5% of chlorella extract, 0.01-0.5% of cactus extract, and centella asiatica extract: 0.3-0.8% of golden yellow chamomile extract, 0.01-0.5% of sodium hyaluronate, 0.15-0.4% of tremella polysaccharide, 1-4% of trehalose, 0.3-1.2% of sclerotium rolfsii gum and the balance of water.
The invention also provides a preparation method of the eye mask containing the tremella polysaccharide, which comprises the following steps:
(1) heating and stirring humectant, thickener, antiseptic, Tremella polysaccharide, sclerotium rolfsii gum and water until completely dissolved;
(2) pouring the mixture into an eye mask mold, cooling, solidifying and demolding to obtain the eye mask containing the Tremella polysaccharide.
The invention also provides a preparation method of the wrinkle-removing eye mask with moisturizing, anti-aging and refreshing effects, which comprises the following steps:
a. heating and stirring humectant, thickener, antiseptic, Tremella polysaccharide, trehalose, Sclerotinia sclerotiorum and water to dissolve completely;
b. adding Chlorella extract, herba Centellae extract, radix et caulis Opuntiae Dillenii extract, flos Chrysanthemi extract, and sodium hyaluronate into the above mixture, and mixing;
c. and (c) pouring the mixture obtained in the step (b) into an eye mask mold, cooling, solidifying and demolding to obtain the wrinkle-removing eye mask.
Further, in the step (1), the components are stirred at 80-90 ℃ until completely dissolved.
The eye mask obtained by the invention does not need base materials such as eye mask cloth or eye mask paper, the eye mask can be directly obtained by mixing and molding the components, and the obtained eye mask is in a gel state, has good fitting property with eye skin, is not easy to break, has good mechanical property and is convenient to mold and use.
The invention has the following beneficial effects:
1. according to the eye mask, the tremella polysaccharide and the sclerotium rolfsii gum are added, so that the wrinkle removing effect of the eyes can be obviously improved through the compounding of the tremella polysaccharide and the sclerotium rolfsii gum, the mechanical property of the eye mask can be greatly improved, the eye mask is more conformable, not easy to break, good in forming effect and convenient to use;
2. the eye mask can be added with other effective components according to the special characteristics of eye skin, so that the efficacy of the eye mask is more diversified. For example, the eye skin can be compacted, wrinkles can be effectively removed, eye moisture can be locked, water loss can be prevented, the resistance of the eye skin can be enhanced, the aging can be delayed, a weak acid protective film can be formed on the eye skin after long-term use, the toughness of the eye skin can be enhanced, the wrinkle removing, moisturizing and anti-aging effects of the eye skin can be more obvious, and the eye skin can reach the optimal state.
Detailed Description
In order to better explain the technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples. It should be noted that the process parameters, process steps and the like which are not described in detail in the present invention are performed according to the conventional technical means in the field.
In the following examples, unless otherwise specified, all the starting materials and the test reagents were commercially available.
Example 1
Fermented tremella polysaccharide wrinkle-removing eyeThe membrane comprises the following components in percentage by mass: total content of humectant: 8.3% (propylene glycol: 2.9%, glycerin: 3.5%, butylene glycol: 1.8%, allantoin: 0.1%), total thickener content: 4.5% (glucomannan: 2.1%, carbomer: 2.4%), total amount of preservatives: 0.2% (dipotassium glycyrrhizinate: 0.1%, methylparaben: 0.1%), tremella polysaccharide: 0.15 percent, 1.2 percent of sclerotium rolfsii gum and the balance of water. The specific molecular weight of Tremella polysaccharide is 3.67 × 106Da。
The preparation method of the fermented tremella polysaccharide wrinkle-removing eye mask comprises the following steps:
the method comprises the following steps: preparing a tremella spore fermentation extract: taking a tremella spore strain preserved by glycerol (the preservation number of the strain is CCTCC M2017271), inoculating the tremella spore strain in 1L TPS culture medium with the liquid loading capacity of 200mL, performing shake cultivation for 4d under the conditions of 28 ℃ and 200rpm/min, transferring the tremella spore strain into a 5L fermentation tank, and performing fermentation cultivation for 7d at 28 ℃ and 800rpm/min to obtain the high-molecular tremella polysaccharide fermentation liquor. Diluting the prepared fermentation broth, filtering, precipitating the filtrate with ethanol for 10-12 hr, removing supernatant, collecting precipitate, and drying in vacuum drying oven at room temperature to obtain high molecular weight Tremella polysaccharide granule with molecular weight of 3.67 × 106Da, grinding into powder for later use;
step two: adding the humectant, the thickener, the preservative, the high-molecular tremella polysaccharide particles, the sclerotium rolfsii gum and the water into an emulsifying pot according to the formula ratio, heating to 80-90 ℃, and dispersing and stirring at a high speed until the materials are completely dissolved;
step three: and (5) pouring the mixture prepared in the step two into an eye mask mould, cooling and solidifying, demoulding and packaging to obtain the finished product of the wrinkle-removing eye mask.
Example 2
Wrinkle-removing eye masks were prepared according to the formulation and method of example 1, except that: the content of tremella polysaccharide is 0.4 wt%, and the content of sclerotium rolfsii gum is 0.3 wt%.
Example 3
Wrinkle-removing eye masks were prepared according to the formulation and method of example 1, except that: the content of tremella polysaccharide is 0.25 wt%, and the content of sclerotium rolfsii gum is 0.8 wt%.
Example 4
Formulation according to example 3And method of making a wrinkle-removing eye mask, except that: adjusting the fermentation preparation steps of Tremella polysaccharide with molecular weight of 4.5 × 106Da。
Example 5
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: adjusting the fermentation preparation step of Tremella polysaccharide with molecular weight of 0.83 × 106Da。
Example 6
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: adjusting the fermentation preparation steps of Tremella polysaccharide with molecular weight of 4.9 × 106Da。
Example 7
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: the Tremella polysaccharide is prepared by extraction method, and has molecular weight of 3.73 × 106Da。
Example 8
A fermented tremella polysaccharide wrinkle-removing eye mask comprises the following components in percentage by mass: total content of humectant: 8.3% (propylene glycol: 2.9%, glycerin: 3.5%, butylene glycol: 1.8%, allantoin: 0.1%), total thickener content: 4.5% (glucomannan: 2.1%, carbomer: 2.4%), total amount of preservatives: 0.2% (dipotassium glycyrrhizinate: 0.1%, methylparaben: 0.1%), tremella polysaccharide: 0.25 percent, sclerotium rolfsii gum 0.8 percent, trehalose 3 percent, sodium hyaluronate 0.2 percent, centella asiatica extract 0.6 percent and the balance of water. The specific molecular weight of Tremella polysaccharide is 3.67 × 106Da。
The preparation method of the fermented tremella polysaccharide wrinkle-removing eye mask comprises the following steps:
the method comprises the following steps: high molecular weight Tremella polysaccharide granules with a molecular weight of 3.67 × 10 were obtained as in example 16Da, grinding into powder for later use;
step two: adding the humectant, the thickener, the preservative, the high-molecular tremella polysaccharide particles, the sclerotium rolfsii gum, the trehalose and the water into an emulsifying pot according to the formula ratio, heating to 80-90 ℃, and dispersing and stirring at a high speed until the tremella polysaccharide particles are completely dissolved;
step three: adding herba Centellae extract and sodium hyaluronate into emulsifying pot, and mixing;
step four: and pouring the mixture prepared in the third step into an eye mask mold, cooling and solidifying, demolding and packaging to obtain the finished product of the wrinkle-removing eye mask.
Example 9
A fermented tremella polysaccharide wrinkle-removing eye mask comprises the following components in percentage by mass: total content of humectant: 8.3% (propylene glycol: 2.9%, glycerin: 3.5%, butylene glycol: 1.8%, allantoin: 0.1%), total thickener content: 4.5% (glucomannan: 2.1%, carbomer: 2.4%), total amount of preservatives: 0.2% (dipotassium glycyrrhizinate: 0.1%, methylparaben: 0.1%), tremella polysaccharide: 0.25 percent, 0.8 percent of sclerotium rolfsii gum, 0.2 percent of chlorella extract, 0.2 percent of cactus extract, 0.2 percent of golden yellow chamomile extract and the balance of water. The specific molecular weight of Tremella polysaccharide is 3.67 × 106Da。
The preparation method of the fermented tremella polysaccharide wrinkle-removing eye mask comprises the following steps:
the method comprises the following steps: high molecular weight Tremella polysaccharide granules with a molecular weight of 3.67 × 10 were obtained as in example 16Da, grinding into powder for later use;
step two: adding the humectant, the thickener, the preservative, the high-molecular tremella polysaccharide particles, the sclerotium rolfsii gum and the water into an emulsifying pot according to the formula ratio, heating to 80-90 ℃, and dispersing and stirring at a high speed until the materials are completely dissolved;
step three: adding Chlorella extract, radix et caulis Opuntiae Dillenii extract, and flos Chrysanthemi extract into emulsifying pot, and mixing;
step four: and pouring the mixture prepared in the third step into an eye mask mold, cooling and solidifying, demolding and packaging to obtain the finished product of the wrinkle-removing eye mask.
Example 10
A fermented tremella polysaccharide wrinkle-removing eye mask comprises the following components in percentage by mass: total content of humectant: 8.3% (propylene glycol: 2.9%, glycerin: 3.5%, butylene glycol: 1.8%, allantoin: 0.1%), total thickener content: 4.5% (glucomannan: 2.1%, carbomer:2.4%), total amount of preservative: 0.2% (dipotassium glycyrrhizinate: 0.1%, methylparaben: 0.1%), tremella polysaccharide: 0.25 percent of sclerotium rolfsii gum, 0.8 percent of trehalose, 3 percent of sodium hyaluronate, 0.6 percent of centella extract, 0.2 percent of chlorella extract, 0.2 percent of cactus extract, 0.2 percent of golden yellow chamomile extract and the balance of water. The specific molecular weight of Tremella polysaccharide is 3.67 × 106Da。
The preparation method of the fermented tremella polysaccharide wrinkle-removing eye mask comprises the following steps:
the method comprises the following steps: high molecular weight Tremella polysaccharide granules with a molecular weight of 3.67 × 10 were obtained as in example 16Da, grinding into powder for later use;
step two: adding the humectant, the thickener, the preservative, the high-molecular tremella polysaccharide particles, the sclerotium rolfsii gum, the trehalose and water into an emulsifying pot according to the formula ratio, heating to 80-90 ℃, and dispersing and stirring at a high speed until the tremella polysaccharide particles are completely dissolved;
step three: adding sodium hyaluronate, herba Centellae extract, Chlorella extract, radix et caulis Opuntiae Dillenii extract, and flos Chrysanthemi extract into emulsifying pot, and mixing;
step four: and pouring the mixture prepared in the third step into an eye mask mold, cooling and solidifying, demolding and packaging to obtain the finished product of the wrinkle-removing eye mask.
Comparative example 1
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: the content of tremella polysaccharide is 0.15 wt%, and the content of sclerotium rolfsii gum is 1.5 wt%.
Comparative example 2
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: the content of tremella polysaccharide is 1.2 wt%, and the content of sclerotium rolfsii gum is 0.2 wt%.
Comparative example 3
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: no sclerotium rolfsii gum is added.
Comparative example 4
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: tremella polysaccharide is not added.
Comparative example
Wrinkle-removing eye masks were prepared according to the formulation and method of example 3, except that: tremella polysaccharides and sclerotium rolfsii gum are not added.
To verify the wrinkle-removing and moisturizing effects of the present invention, the following experiments were performed:
1. an experimental instrument: human skin rapid three-dimensional imaging system (PRIMOS, germany), skin moisture tester Corneometer CM 825(Courage + Khazaka corporation, germany).
2. The test method comprises the following steps: the eye masks prepared in the examples and the comparative examples were compared with the eye mask of the comparative example, and the eye mask of the examples or the comparative examples was half-used and the eye mask of the comparative example was half-used in a half-face comparison manner, once a day for 15min each time. 10 volunteers, age 30-50 years, were recruited per experimental sample, male and female unlimited.
3. Evaluation of wrinkle-removing Effect: the method comprises the steps of measuring the wrinkle depth of the canthus 30min, 1h, 3h and 6h before and after the first use by adopting a rapid three-dimensional imaging system for the skin of a human body, calculating the relative value of the wrinkle depth after the use by taking the wrinkle depth before the use as 100%, and averaging the relative values of the wrinkle depth of the same sample to evaluate the short-term wrinkle removing effect.
The wrinkle depth was measured before and after 4 weeks of continuous use for 1 week, 2 weeks, 3 weeks and 4 weeks, relative wrinkle depth values after use were calculated based on the wrinkle depth before use as 100%, and relative wrinkle depth values using the same sample were averaged to evaluate the long-term wrinkle removal effect.
Relative wrinkle depth value is 100% post-use wrinkle depth/pre-use wrinkle depth.
4. Evaluation of moisturizing effect: the moisture content of the eyes was measured before and after 1 week, 2 weeks, 3 weeks, 4 weeks using a skin moisture tester.
Skin moisture content relative (%) as measured after use/initial value before use × 100%
5. Evaluation of skin elasticity effect: the eye skin elasticity before and after 1 week, 2 weeks, 3 weeks and 4 weeks of use is measured by adopting a rapid three-dimensional imaging system of human skin, and the skin elasticity change rate is calculated.
The skin elasticity change rate is calculated by: change rate (%) [ (skin elasticity after use-skin elasticity before use)/skin elasticity before use ] × 100%.
6. Test results
6.1 short-term wrinkle-removing effect is shown in Table 1:
TABLE 1
6.2 Long-term wrinkle-removing effects are shown in Table 2:
TABLE 2
6.3 moisturizing effect is shown in table 3 below:
TABLE 3
6.4 skin elasticity effect is shown in Table 4 below:
TABLE 4
The experimental results show that when the tremella polysaccharide or the sclerotium rolfsii gum is added independently, the wrinkle removing effect, the moisturizing effect and the skin elasticity effect are far from those of the tremella polysaccharide or the sclerotium rolfsii gum when the tremella polysaccharide or the sclerotium rolfsii gum is added, so that the sclerotium rolfsii gum and the tremella polysaccharide can be synergized, and the effects of removing wrinkles, skin elasticity and moisturizing are greatly improved.
From examples 1-7, Tremella polysaccharidesThe molecular weight is 3.5X 106～4.5×106The eye mask has better performance in Da, and the tremella polysaccharide obtained by the extraction method has slightly lower performance in wrinkle removal, skin elasticity and the like than the tremella polysaccharide obtained by the fermentation method, which indicates that the source of the tremella polysaccharide has little influence on the performance of the eye mask, and the tremella polysaccharide obtained by the fermentation method has better performance.
To verify the mechanical properties of the eye mask of the invention, the following experiments were carried out:
and (3) measuring the adhesion force:
1. the test instrument: instron instruments (Instron-3365).
2. The test method comprises the following steps: the eye film was attached to the eye and immediately after attachment the eye film was peeled off from the skin using an Instron instrument (Instron-3365) and the adhesion was measured.
Tensile strength was determined according to GB/T1040.2-2006.
The results of the experiment are shown in table 5 below.
TABLE 5
| Experimental sample | Adhesion (mN) | Tensile Strength (MPa) |
| Example 1 | 26.4 | 43.06 |
| Example 2 | 25.9 | 43.58 |
| Example 3 | 27.6 | 45.38 |
| Example 4 | 25.6 | 43.89 |
| Example 5 | 23.5 | 41.29 |
| Example 6 | 24.9 | 40.87 |
| Example 7 | 22.6 | 39.75 |
| Example 8 | 24.3 | 43.31 |
| Example 9 | 23.6 | 43.15 |
| Example 10 | 23.8 | 42.94 |
| Comparative example 1 | 22.7 | 37.93 |
| Comparative example 2 | 23.2 | 38.41 |
| Comparative example 3 | 15.4 | 24.17 |
| Comparative example 4 | 14.8 | 25.61 |
| Comparative example | 8.7 | 20.18 |
The experimental results show that when tremella polysaccharide or sclerotium rolfsii gum is added independently, the prepared eye mask is not conformable enough, the adhesion and tensile strength of the eye mask are low, and the eye mask is easy to damage. When the tremella polysaccharide and the sclerotium rolfsii gum are added simultaneously, the eye mask can achieve the optimal fitting effect, the adhesion and the tensile strength are greatly improved, the forming effect is good, and the eye mask is not easy to break. The sclerotium rolfsii gum can be synergized with the tremella polysaccharide, and has a better effect on the mechanical property of the eye mask.
The above examples are merely illustrative of the present invention and do not limit the scope of the present invention in any way. It will be apparent to those skilled in the art that equivalent embodiments or modifications without departing from the technical spirit of the present invention are within the scope of the present invention.
Claims (10)
1. An eye mask comprising a Tremella polysaccharide, characterized by: comprises the following effective components in percentage by weight: 0.15-0.4% of tremella polysaccharide and 0.3-1.2% of sclerotium rolfsii gum.
2. The ophthalmic film comprising Tremella polysaccharide of claim 1, wherein: the molecular weight of the tremella polysaccharide is as follows: 0.8X 106～4.9×106Da, preferably 3.5X 106～4.5×106Da。
3. The ophthalmic film comprising Tremella polysaccharide of claim 1 or 2, wherein: the tremella polysaccharide is obtained by a biological fermentation method, and is preferably obtained by submerged fermentation of tremella spore liquid.
4. The ophthalmic film comprising Tremella polysaccharide of claim 1, wherein: also comprises one or more of a moisturizing component, an anti-aging component, a whitening component, a freckle removing component and a bacteriostatic component.
5. The ophthalmic film comprising Tremella polysaccharide of claim 1 or 4, wherein: also comprises one or more of the following active ingredients: chlorella extract, cactus extract, centella asiatica extract, golden chamomile extract, sodium hyaluronate and trehalose.
6. The ophthalmic film comprising Tremella polysaccharides of claim 5, wherein: the content of the chlorella extract is 0.01-0.5 wt%, the content of the cactus extract is 0.01-0.5 wt%, the content of the centella extract is 0.3-0.8 wt%, the content of the golden yellow chamomile extract is 0.01-0.5 wt%, the content of the sodium hyaluronate is 0.1-0.8 wt%, and the content of the trehalose is 1-4 wt%.
7. The ophthalmic film comprising Tremella polysaccharides of claim 1, 4, 5 or 6, wherein: the water-based paint also comprises an auxiliary material component, wherein the auxiliary material component is at least one selected from a humectant, a thickening agent, a preservative and water.
8. The ophthalmic film comprising Tremella polysaccharide of claim 7, wherein: the humectant is selected from: one or more of propylene glycol, glycerin, butylene glycol, and allantoin; the thickening agent is selected from carbomer or/and glucomannan; the preservative is selected from dipotassium glycyrrhizinate or/and methyl hydroxybenzoate.
9. The ophthalmic film comprising a Tremella polysaccharide of claim 1, 2, 4, 5, or 6, wherein: the eye mask is a gel eye mask paste.
10. A method of preparing an ophthalmic lens comprising a Tremella polysaccharide according to any one of claims 1-9, comprising the steps of:
(1) heating and stirring humectant, thickener, antiseptic, Tremella polysaccharide, trehalose, Sclerotinia sclerotiorum and water to dissolve completely;
(2) adding Chlorella extract, herba Centellae extract, radix et caulis Opuntiae Dillenii extract, flos Chrysanthemi extract, and sodium hyaluronate into the above mixture, and mixing;
(3) and (3) pouring the mixture obtained in the step (2) into an eye mask mold, cooling, solidifying and demolding to obtain the eye mask containing the Tremella polysaccharide.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113018232A (en) * | 2021-03-10 | 2021-06-25 | 福建师范大学 | Eye cream containing hibiscus flower extract and preparation method thereof |
| CN114146017A (en) * | 2021-11-12 | 2022-03-08 | 广州科妍化妆品有限公司 | Freeze-dried eye patch and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060222608A1 (en) * | 2005-04-01 | 2006-10-05 | Agricultural Research Institute | Edible tremella polysaccharide for skin care |
| JP2012241006A (en) * | 2011-05-24 | 2012-12-10 | Kao Corp | Oil-in-water type emulsion makeup cosmetic |
| CN105342873A (en) * | 2015-11-02 | 2016-02-24 | 上海韵宜生物科技有限公司 | Novel skin care gel as well as preparation method and application thereof |
| CN106974842A (en) * | 2017-05-12 | 2017-07-25 | 朝日化妆品(深圳)有限公司 | A kind of tremella polysaccharides facial mask |
| CN107961191A (en) * | 2017-12-28 | 2018-04-27 | 广州蜜妆生物科技有限公司 | A kind of kahikatea dew anti-aging is compacted eye mask and preparation method thereof |
| CN110585030A (en) * | 2019-09-27 | 2019-12-20 | 广州香枝化妆品有限公司 | High-molecular gel wrinkle-removing eye mask and preparation method thereof |
-
2020
- 2020-10-30 CN CN202011185808.3A patent/CN112137895A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060222608A1 (en) * | 2005-04-01 | 2006-10-05 | Agricultural Research Institute | Edible tremella polysaccharide for skin care |
| JP2012241006A (en) * | 2011-05-24 | 2012-12-10 | Kao Corp | Oil-in-water type emulsion makeup cosmetic |
| CN105342873A (en) * | 2015-11-02 | 2016-02-24 | 上海韵宜生物科技有限公司 | Novel skin care gel as well as preparation method and application thereof |
| CN106974842A (en) * | 2017-05-12 | 2017-07-25 | 朝日化妆品(深圳)有限公司 | A kind of tremella polysaccharides facial mask |
| CN107961191A (en) * | 2017-12-28 | 2018-04-27 | 广州蜜妆生物科技有限公司 | A kind of kahikatea dew anti-aging is compacted eye mask and preparation method thereof |
| CN110585030A (en) * | 2019-09-27 | 2019-12-20 | 广州香枝化妆品有限公司 | High-molecular gel wrinkle-removing eye mask and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113018232A (en) * | 2021-03-10 | 2021-06-25 | 福建师范大学 | Eye cream containing hibiscus flower extract and preparation method thereof |
| CN114146017A (en) * | 2021-11-12 | 2022-03-08 | 广州科妍化妆品有限公司 | Freeze-dried eye patch and preparation method thereof |
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