CN103125735A - Preparation method of walnut polypeptide - Google Patents
Preparation method of walnut polypeptide Download PDFInfo
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- CN103125735A CN103125735A CN 201310075875 CN201310075875A CN103125735A CN 103125735 A CN103125735 A CN 103125735A CN 201310075875 CN201310075875 CN 201310075875 CN 201310075875 A CN201310075875 A CN 201310075875A CN 103125735 A CN103125735 A CN 103125735A
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Abstract
The invention provides a preparation method of walnut polypeptide. The preparation method comprises the following steps of: a, treatment of raw materials: uniformly mixing smashed material of walnut cake which is cold-pressed, degreased at a low temperature with water to obtain a walnut cake suspension; b, homogenization of the suspension: homogenizing the suspension to obtain a walnut cake mixed liquid; c, heat-insulating enzymolysis: adding a mixed enzyme liquid of AlcaLase enzyme and papain to the walnut cake mixed liquid, carrying out enzymolysis to prepare the walnut cake enzymatic hydrolysate under the enzymolysis conditions that the temperature is 50-60 DEG C, the pH value is 7.0-8.5 and the time is 6-8 hours; d, high-temperature sterilization: quickly heating the walnut cake enzymatic hydrolysate to 90-59 DEG C; e, liquid-solid separation: carrying out liquid-solid separation on walnut cake enzymatic hydrolysate after enzyme deactivation at a high temperature, removing insoluble solids to prepare a walnut polypeptide liquid; f, concentration: concentrating the walnut polypeptide liquid to obtain walnut polypeptide concentrated liquor; and g, drying: drying the walnut polypeptide concentrated liquor to obtain the walnut polypeptide.
Description
Technical field
The invention belongs to food processing field, especially a kind of preparation method of walnut polypeptide.
Background technology
Walnut has another name called English walnut, Qiang fruit, is Juglandaceae (Juglandaceae) Juglans (Juglans), originates in the areas such as W. Asia, West Asia, now in the North America, the extensively cultivations of area such as north African, East Asia, China's most area has distribution.Walnut and almond, cashew nut, fibert are listed as the large dry fruit in the world four, have very high edibility, are indispensable dry fruits on western countries' Christmas Day.Walnut not only edibility is very high, and health care is very outstanding.Abroad, walnut is called as " brain tonic is fruit ", " long-lived fruit ", " beauty treatment is fruit ".Phosphatide in walnut kernel has excellent health functions to cranial nerve.The walnut oil that extracts contains a large amount of unrighted acids, and the arteriosclerotic effect of control is arranged
[5]Contain the indispensable trace elements of human body such as zinc, manganese, chromium in walnut kernel, can day by day reduce human body zinc, manganese content in aging course and replenish.The medical value of walnut is also very high, and the traditional Chinese medical science is widely used.Motherland's medical science thinks that walnut is warm in nature, flavor is sweet, nontoxic, stomach invigorating is arranged, enriches blood, moistening lung, the effect such as repose.
Walnut protein content is generally 14%～l7%, reaches as high as 29.7%, and it tires close with animal protein, is a kind of good protein.The walnut protein amino acid content is abundant, and 18 seed amino acid content are reasonable, near FAO (Food and Agriculture Organization of the United Nation) (FAO) and the World Health Organization's specified standard, 8 kinds of necessary amino acid is arranged wherein, and the content of arginine and glutamic acid is all very high.Walnut protein is made of four kinds of protein, and they are albumin, globulin, prolamin and glutelin, accounts for respectively walnut protein total amount 6.81%, 17.57%, 5.33% and 70.11%.Walnut is a kind of health products that are rich in high unsaturated fatty acid, and the ratio of the digestibility of its gross protein and clean albumen is higher, tires close with animal protein, and amino acid content is abundant, is a kind of good protein resource.
The petal resource of China is very abundant, but the degree of depth of current exploitation also is nowhere near, and only is confined to the making of Elementary food, and added value of product is too low.Only have walnut is carried out deep processing, the product that exploitation has high technology content could allow the resource interests larger for we bring of this preciousness.Present domestic walnut oil manufacturer has reached tens of families, and a large amount of degreasing walnut dregs of rice are used as feed and fertilizer or abandon, the waste discharge contaminated environment, and its added value is lower.For the hickory nut resource that takes full advantage of China and improve its economic worth, the degreasing walnut dregs of rice are carried out deep processing research seem particularly important.Therefore, utilize the albumen in the protease hydrolytic walnut dregs to prepare walnut polypeptide powder, realized utilizing walnut dregs to improve the purpose of its added value.Simultaneously, prepared walnut polypeptide powder have strengthen body immunity, anti-ageing, anti-oxidant, the function such as set up.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method of walnut polypeptide, solves the degradation problem of high-fat walnut protein, can be with direct absorb, the nutritious walnut polypeptide powder of degreasing walnut human body.Above-mentioned technical problem the technical solution adopted in the present invention is in order to determine:
A kind of preparation method of walnut polypeptide, this preparation method comprises the following steps:
A, raw material selected: normal take color, be as good as without a defatted walnut cake that goes mouldy as raw material;
The pretreatment of b, raw material: the walnut cakes crushed material after defatted walnut cake is pulverized and water mix and make the walnut cakes suspension;
The homogeneous of c, suspension: walnut cakes suspension homogeneous is made the walnut cakes mixed liquor;
D, insulation enzymolysis: the mixed enzyme solution that adds AlcaLase enzyme and papain in the walnut cakes mixed liquor, the addition of mixed enzyme solution is 0.03%～0.05% of walnut cakes mixed liquor quality, enzyme hydrolysis condition is that temperature is 50～60 ℃, pH value 7.0～8.5,6～8 hours time, make the walnut cakes enzymolysis liquid;
E, high-temperature sterilization: the temperature of walnut cakes enzymolysis liquid is rapidly heated to 90～95 ℃, and is incubated 8～10 minutes;
Gu f, liquid-separation: Gu will carry out removing insoluble solid after liquid-separation through the go out walnut cakes enzymolysis liquid of enzyme of high temperature, make walnut polypeptide liquid;
G, concentrated: with the concentrated walnut polypeptide concentrate that makes of walnut polypeptide liquid;
H, drying: the walnut polypeptide concentrate is dry, namely obtain walnut polypeptide powder.
In step b, make the walnut cakes crushed material defatted walnut cake is pulverized and cross 40 mesh sieves with pulverizer after, then walnut cakes crushed material and water are made the walnut cakes suspension after the ratio of 1:4～6 mixes.
In described step c, the walnut cakes suspension is processed by the high pressure homogenizer homogeneous after by the colloid mill defibrination again made the walnut cakes mixed liquor, homogenization pressure is 25～30Mpa.
In described steps d, the AlcaLase enzyme of mixed enzyme solution and the volume ratio of papain are AlcaLase enzyme: papain=1:1..
In described step f, Gu adopt disk plate centrifuge to carry out liquid-separation to peach cake enzymolysis liquid.
In described step g, adopt Vacuum Concentration to make the walnut polypeptide concentrate, the moisture of walnut polypeptide concentrate is 65～70%.
In described step h, adopt spray-dired mode to make walnut polypeptide powder.
The present invention compared with prior art has following advantage and effect:
(1) prepare the high-load walnut protein with " the molten acid of alkali is heavy ", provide high-quality feedstocks for extracting the walnut biologically active polypeptide.
(2) adopt ultrafiltration to carry out classification to walnut polypeptide and process, guarantee the Peanut Polypeptide molecular weight of 95 % below 3 000 Da, and carry out secondary enzymolysis for the walnut polypeptide more than molecular weight 3 000 Da, improve the utilization rate of raw material;
(3) utilize the negative and positive exchanger resin to carry out desalination to the walnut polypeptide crude product, in desalination, also can remove the pigment composition in walnut polypeptide, play the effect of decolouring;
(5) walnut polypeptide that is prepared and made with extra care by the present invention, molecular weight is low, product stability good, has good antifatigue, hypotensive, anti-oxidant texts.
The specific embodiment
Embodiment
A kind of preparation method of walnut polypeptide, the method comprises the steps:
The processing of a, raw material: make the walnut cakes crushed material defatted walnut cake is pulverized and cross 40 mesh sieves with pulverizer after, then walnut cakes crushed material and water are made the walnut cakes suspension after the ratio of 1:4 mixes.
The homogeneous of b, suspension: the walnut cakes suspension is processed by the high pressure homogenizer homogeneous after by the colloid mill defibrination again made the walnut cakes mixed liquor, homogenization pressure is 30Mpa.
C, insulation enzymolysis: add the mixed enzyme solution of AlcaLase enzyme and papain in the walnut cakes mixed liquor,
The AlcaLase enzyme of mixed enzyme solution and the volume ratio of papain are the AlcaLase enzyme: the addition of papain=1:1. mixed enzyme solution is 0.05% of walnut cakes mixed liquor quality, enzyme Sui Jie condition is that temperature is 60 ℃, pH value 7.0,8 hours time, make the walnut cakes enzymolysis liquid;
D, high-temperature sterilization: the temperature of walnut cakes enzymolysis liquid is rapidly heated to 95 ℃, and is incubated 10 minutes;
Gu e, liquid-separation: Gu will carry out removing insoluble solid after liquid-separation through the go out walnut cakes enzymolysis liquid of enzyme of high temperature, make walnut polypeptide liquid;
F, concentrated: adopt Vacuum Concentration to make the walnut polypeptide concentrate walnut polypeptide liquid, the moisture of walnut polypeptide concentrate is 70%.The concentrated walnut polypeptide concentrate that makes;
G, drying: adopt spray-dired mode to make the walnut walnut polypeptide powder walnut polypeptide concentrate.
Claims (7)
1. the preparation method of a walnut polypeptide, is characterized in that comprising the steps:
The processing of a, raw material: the walnut cakes crushed material after the low-temperature cold pressing defatted walnut cake is pulverized and water mix and are worth the walnut cakes suspension;
The homogeneous of b, suspension: walnut cakes suspension homogeneous is made the walnut cakes mixed liquor;
C, insulation enzymolysis: add the mixed enzyme solution of AlcaLase enzyme and papain in the walnut cakes mixed liquor, the addition of mixed enzyme solution is 0.03%～0.05% of walnut cakes mixed liquor quality, enzyme Sui Jie condition be temperature be 50～60 ℃, pH value 7.0～
8.5,6～8 hours time, make the walnut cakes enzymolysis liquid;
D, high-temperature sterilization: the temperature of walnut cakes enzymolysis liquid is rapidly heated to 90～95 ℃, and is incubated 8～10 minutes;
Gu e, liquid-separation: Gu will carry out removing insoluble solid after liquid-separation through the go out walnut cakes enzymolysis liquid of enzyme of high temperature, make walnut polypeptide liquid;
F, concentrated: with the concentrated walnut polypeptide concentrate that makes of walnut polypeptide liquid;
G, drying: the walnut polypeptide concentrate is dry, namely obtain walnut polypeptide powder.
2. the preparation method of a kind of walnut polypeptide according to claim 1 is characterized in that: in step b, make the walnut cakes crushed material defatted walnut cake is pulverized and cross 40 mesh sieves with pulverizer after, then with walnut cakes crushed material and water by weight 1:4～
After mixing, 6 ratio makes the walnut cakes suspension.
3. the preparation method of a kind of walnut polypeptide according to claim 1 is characterized in that: in step c, the walnut cakes suspension is processed by the high pressure homogenizer homogeneous after by the colloid mill defibrination again made the walnut cakes mixed liquor, homogenization pressure is 25～30Mpa.
4. the preparation method of a kind of walnut polypeptide according to claim 1, it is characterized in that: in steps d, the AlcaLase enzyme of mixed enzyme solution and the volume ratio of papain are AlcaLase enzyme: papain=1:1.
5. the preparation method of a kind of walnut polypeptide according to claim 1, is characterized in that: in step f, Gu adopt disk plate centrifuge to carry out liquid-separation to peach cake enzymolysis liquid.
6. the preparation method of a kind of walnut polypeptide according to claim 1 is characterized in that: in step g, adopt Vacuum Concentration to make the walnut polypeptide concentrate, the moisture of walnut polypeptide concentrate is 65～70%.
7. the preparation method of a kind of walnut polypeptide according to claim 1, is characterized in that: in step h, adopt spray-dired mode to make walnut polypeptide powder.
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| CN 201310075875 CN103125735A (en) | 2013-03-11 | 2013-03-11 | Preparation method of walnut polypeptide |
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| CN 201310075875 CN103125735A (en) | 2013-03-11 | 2013-03-11 | Preparation method of walnut polypeptide |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468773A (en) * | 2013-09-06 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Extraction method of walnut kernel polypeptide |
| CN103652314A (en) * | 2013-12-06 | 2014-03-26 | 陈栋梁 | Microencapsulated walnut peptide and preparation method thereof |
| CN104031968A (en) * | 2014-06-27 | 2014-09-10 | 广西壮族自治区中国科学院广西植物研究所 | Walnut polypeptide and preparation method and application thereof |
| CN104172413A (en) * | 2014-08-07 | 2014-12-03 | 陕西天玉实业有限公司 | Preparation method of walnut polypeptide beverage |
| CN104531337A (en) * | 2014-12-16 | 2015-04-22 | 山东乐悠悠花生油科技有限公司 | Method for continuously preparing tannin, walnut oil and protein powder by using walnuts |
| CN105567774A (en) * | 2016-01-22 | 2016-05-11 | 杏辉天力（杭州）药业有限公司 | Walnut oligopeptide powder and preparation method and application thereof |
| CN107712769A (en) * | 2017-11-01 | 2018-02-23 | 安徽省林锦记食品工业有限公司 | A kind of processing method of walnut polypeptide honeydew jelly |
| CN109055462A (en) * | 2018-08-06 | 2018-12-21 | 河南丰之源生物科技有限公司 | A kind of extraction and preparation process of walnut polypeptide |
| CN109984245A (en) * | 2019-04-16 | 2019-07-09 | 中国热带农业科学院南亚热带作物研究所 | A kind of Queensland nut protein peptides and its preparation method and application having anti-fatigue effect |
| CN113854398A (en) * | 2021-10-08 | 2021-12-31 | 长春大学 | Method for preparing digestion-free walnut protein by using walnut cake meal as raw material |
-
2013
- 2013-03-11 CN CN 201310075875 patent/CN103125735A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468773A (en) * | 2013-09-06 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Extraction method of walnut kernel polypeptide |
| CN103652314A (en) * | 2013-12-06 | 2014-03-26 | 陈栋梁 | Microencapsulated walnut peptide and preparation method thereof |
| CN103652314B (en) * | 2013-12-06 | 2015-07-15 | 陈栋梁 | Microencapsulated walnut peptide and preparation method thereof |
| CN104031968A (en) * | 2014-06-27 | 2014-09-10 | 广西壮族自治区中国科学院广西植物研究所 | Walnut polypeptide and preparation method and application thereof |
| CN104172413A (en) * | 2014-08-07 | 2014-12-03 | 陕西天玉实业有限公司 | Preparation method of walnut polypeptide beverage |
| CN104531337A (en) * | 2014-12-16 | 2015-04-22 | 山东乐悠悠花生油科技有限公司 | Method for continuously preparing tannin, walnut oil and protein powder by using walnuts |
| CN105567774A (en) * | 2016-01-22 | 2016-05-11 | 杏辉天力（杭州）药业有限公司 | Walnut oligopeptide powder and preparation method and application thereof |
| CN105567774B (en) * | 2016-01-22 | 2019-09-10 | 杏辉天力（杭州）药业有限公司 | A kind of oligomeric Gly-His-Lys of walnut and its preparation method and application |
| US11072637B2 (en) | 2016-01-22 | 2021-07-27 | Sinphar Tian-Li (Hangzhou) Pharmacy Company Limited | Walnut oligopeptide powder, preparation method and application thereof |
| CN107712769A (en) * | 2017-11-01 | 2018-02-23 | 安徽省林锦记食品工业有限公司 | A kind of processing method of walnut polypeptide honeydew jelly |
| CN109055462A (en) * | 2018-08-06 | 2018-12-21 | 河南丰之源生物科技有限公司 | A kind of extraction and preparation process of walnut polypeptide |
| CN109984245A (en) * | 2019-04-16 | 2019-07-09 | 中国热带农业科学院南亚热带作物研究所 | A kind of Queensland nut protein peptides and its preparation method and application having anti-fatigue effect |
| CN113854398A (en) * | 2021-10-08 | 2021-12-31 | 长春大学 | Method for preparing digestion-free walnut protein by using walnut cake meal as raw material |
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Application publication date: 20130605 |