CN103468773A - Extraction method of walnut kernel polypeptide - Google Patents
Extraction method of walnut kernel polypeptide Download PDFInfo
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- CN103468773A CN103468773A CN2013104017613A CN201310401761A CN103468773A CN 103468773 A CN103468773 A CN 103468773A CN 2013104017613 A CN2013104017613 A CN 2013104017613A CN 201310401761 A CN201310401761 A CN 201310401761A CN 103468773 A CN103468773 A CN 103468773A
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Abstract
The invention discloses an extraction method of walnut kernel polypeptide. The extraction method comprises the following steps of (1) carrying ball milling and grinding: adding water and papain into deoiled walnut powder, and carrying out wet grinding on the deoiled walnut powder by adopting a ball mill to obtain superfine powder suspension; and (2) carrying out enzyme hydrolysis: separating the suspension from a spherical tank, supplementing water into the solution, adjusting pH to be 6.0-7.0, continuing to add the papain, carrying out ultrasonic treatment for 20-30 minutes under the condition of 55-65 DEG C, and then stirring under the condition of 60 DEG C for the enzyme hydrolysis for 10-30 minutes to obtain the walnut kernel polypeptide through centrifugation. According to the extraction method, the ball milling and superfine grinding, ultrasonic extraction and papain enzymolysis are combined for carrying out superfine grinding, grain refinement and cell disruption on the deoiled walnut powder, macromolecular substances such as proteins are exposed, dissolved and released, and the ultrasonic treatment further promotes the walnut protein to be released from broken cells and dissolved, so that the polypeptide conversion rate is increased significantly.
Description
Technical field
The invention belongs to food or health food processing technique field, relate to a kind of extracting method of walnut kernel polypeptide.
Background technology
Walnut has very high nutritive value and pharmaceutical use, is the very good material of exploitation high-class healthy food.Walnut kernel is containing grease 65%, and protein is more than 15.4%, and after de-oiling, the walnut main component is walnut protein, and walnut polypeptide production mainly be take the de-oiling walnut dregs as raw material, utilizes chemical method or enzymic degradation to form.Enzyme process is compared with traditional acid system, alkaline process processing, there is incomparable advantage, if do not had any hazard residue material, there is height specificity and high efficiency, the zymin consumption is little, the reaction conditions gentleness, the nutritive ingredient loss is few, and easy to operate, energy consumption is low etc., but the Enzymatic Extraction time is long, easy living microorganism.
Natural walnut protein has tight three-dimensional arrangement, and its restriction enzyme site contains in protein interior, thereby is difficult to by protease hydrolysis, so need carry out pre-treatment to walnut protein, makes protein denaturation, effectively to improve the walnut protein degree of hydrolysis.In addition, though conventional enzyme process decomposing protein reaches very high percent hydrolysis, time-consuming and condition harshness, reach the small-molecular peptides stage, and condition is more wayward, so can consider some pre-treatment before the walnut protein enzymolysis.Pretreatment process is more at present, common are microwave, ultrasonic wave, thermal treatment, freeze thawing and ultra-high voltage etc.Present Domestic is outer relevant utilizes pre-treatment to improve plant, animal proteinum degree of hydrolysis relevant report is a lot; But relevant walnut protein report is relatively less.The protein denaturation method has a lot, and wherein thermal treatment is because method is easy, and facility investment is few, becomes the most general pretreatment process.But the thermal treatment energy consumption is large, time is long, the present invention is by ball milling micronizing, supersound extraction, papain enzymolysis three associating, the de-oiling walnut powder is carried out to micronizing, grain refine, cytoclasis, the macromolecular substance such as protein expose to dissolve and discharge, supersound process further impels the walnut protein sex change to discharge dissolving from broken cell, can make that the polypeptide transformation efficiency increases substantially, the enzymolysis and extraction disengaging time shortens greatly.
Summary of the invention
The extracting method that the purpose of this invention is to provide a kind of walnut kernel polypeptide can efficiently obtain fast walnut polypeptide under comparatively gentle condition, makes it reach the advantages such as economical and efficient, environmental protection in production technique.
To achieve these goals, the present invention proposes following technical scheme:
A kind of extracting method of walnut kernel polypeptide comprises the following steps:
(1) ball mill pulverizing: get the de-oiling walnut powder, with de-oiling walnut powder weighing scale, add weight of material 3-5 water doubly, and add papoid 20U/g, adopting ball mill is under the 250-400rpm condition, the de-oiling walnut powder to be carried out to wet pulverization 1-3h to obtain the superfine powder suspension liquid at rotating speed;
(2) enzymic hydrolysis: suspension liquid is separated with spherical tank, with de-oiling walnut powder weighing scale, get 2-3 water doubly and rinse spherical tank, merge suspension liquid 2 times.To amount of makeup water to dry concentration in solution, be 50-100g/l, adjust pH to 6.0-7.0, continue to add the papoid of 20U/g-de-oiling walnut powder, under 55-65 ℃ of condition, press 10-1500W/ and rise ultrasonication 20-30min, centrifugal that supernatant liquor is polypeptide solution after stirring enzymic hydrolysis 10-30min under 60 ℃ of conditions afterwards.
Preferably, in described step (1), ball mill is planetary ball mill.
Preferably, in described step (2), utilize the hydrochloric acid that sodium hydroxide that massfraction is 2% and massfraction are 1% to adjust pH to 6.0-7.0.
Material is: de-oiling walnut powder, papoid (2000U/g).
The present invention adopts papoid (2000U/g), Beijing ancient cooking vessel state biological reagent company limited)
Technical scheme of the present invention, by ball milling micronizing, supersound extraction, papain enzymolysis three associating, the de-oiling walnut powder is carried out to micronizing, grain refine, cytoclasis, the macromolecular substance such as protein expose to dissolve and discharge, and supersound process further impels walnut protein to discharge dissolving from broken cell, and the polypeptide transformation efficiency is increased substantially.Adopt biuret method to detect the content of polypeptide in solution, the transformation efficiency that calculates de-oiling walnut powder polypeptide reaches as high as 87%.
Additional aspect of the present invention and advantage part in the following description provide, and part will become obviously from the following description, or recognize by practice of the present invention.
Embodiment
Below by embodiment, technical scheme of the present invention is described further, but can not be interpreted as limitation of the present invention.
Embodiment 1
Ball mill pulverizing: get the de-oiling walnut powder of 500 grams as in the agate tank, the water that adds weight of material 1500ml, and add papoid 5g, adopting planetary ball mill (XQM-20L, test apparatus institute is analysed by Nanjing section) is under the 400rpm condition, the de-oiling walnut powder to be carried out to wet pulverization 3h to obtain the superfine powder suspension liquid at rotating speed;
Enzymic hydrolysis: suspension liquid is separated with spherical tank, and the water of getting 1500ml rinses spherical tank, merges suspension liquid 2 times.To amount of makeup water to dry concentration in solution, be 100g/l, utilize the hydrochloric acid that sodium hydroxide that massfraction is 2% and massfraction are 1%, adjust pH to 6.5, continue to add the papoid of 5g, under 60 ℃ of conditions, press 1000W/ and rise ultrasonication 30min, further stirring enzymic hydrolysis 30min afterwards.The enzymolysis solution condition is 60 ℃ of conditions, centrifugal that supernatant liquor is polypeptide solution after enzymolysis, adopts biuret method to detect the content of polypeptide in solution, and the transformation efficiency of polypeptide is 87%.Described polypeptide solution can be after filtration, the step of vacuum freezing obtains walnut kernel polypeptide sterling.
The detection method of transformation efficiency: total free aminoacids adopts formol titration; Protein adopts Kjeldahl determination.Polypeptide adopts biuret method to detect.
Polypeptide transformation efficiency calculation formula: transformation efficiency=(T2-T1)/(P1-P2).
In formula: T2 is content of peptides after enzymolysis; T1 is content of peptides before enzymolysis; P1 is total protein content before enzymolysis; P2 is acid-soluble protein content before enzymolysis.
Although illustrated and described embodiments of the invention, those having ordinary skill in the art will appreciate that: in the situation that do not break away from principle of the present invention and aim can be carried out multiple variation, modification, replacement and modification to these embodiment, scope of the present invention is limited by claim and equivalent thereof.
Claims (3)
1. the extracting method of a walnut kernel polypeptide, is characterized in that, comprises the following steps:
(1) ball mill pulverizing: get the de-oiling walnut powder, add weight of material 3-5 water doubly, and add papoid 20U/g, adopting ball mill is under the 250-400rpm condition, the de-oiling walnut powder to be carried out to wet pulverization 1-3h to obtain the superfine powder suspension liquid at rotating speed;
(2) enzymic hydrolysis: suspension liquid is separated with spherical tank, get 2-3 water doubly and rinse spherical tank, merge suspension liquid 2 times,
To amount of makeup water to dry concentration in solution, be 50-100g/l, adjust pH to 6.0-7.0, continue to add the papoid of 20U/g, under 55-65 ℃ of condition, press 10-1500W/ and rise ultrasonication 20-30min, centrifugal that supernatant liquor is polypeptide solution after stirring enzymic hydrolysis 10-30min under 60 ℃ of conditions afterwards.
2. method according to claim 1, is characterized in that, in described step (1), ball mill is planetary ball mill.
3. method according to claim 1, is characterized in that, in described step (2), utilizes the hydrochloric acid that sodium hydroxide that massfraction is 2% and massfraction are 1% to adjust pH to 6.0-7.0.
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| CN2013104017613A CN103468773A (en) | 2013-09-06 | 2013-09-06 | Extraction method of walnut kernel polypeptide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048417A (en) * | 2017-04-17 | 2017-08-18 | 中南林业科技大学 | A kind of preparation method of the peach kernel polypeptide oral liquor of high bioavilability |
| CN107312812A (en) * | 2017-07-11 | 2017-11-03 | 安达市旭朗生物科技有限公司 | A kind of preparation method of ox Cartilage collagen peptide |
| CN108433097A (en) * | 2018-03-02 | 2018-08-24 | 汕头美亨利乐生物科技有限公司 | With the diatery supplement nutriment and preparation method thereof for improving intestinal absorption ability |
| CN109609576A (en) * | 2018-12-29 | 2019-04-12 | 浙江工业大学 | A kind of extracting method of hickory nut polypeptide |
| CN109997944A (en) * | 2019-04-19 | 2019-07-12 | 吉林省东鳌鹿业科技开发有限公司 | A kind of deer tire polypeptide pressed candy and preparation method thereof |
| CN110637879A (en) * | 2019-10-30 | 2020-01-03 | 河北绿岭康维食品有限公司 | Nut roasted seeds and nuts composite antioxidant and method for processing nut roasted seeds and nuts by using same |
| CN113061635A (en) * | 2021-03-31 | 2021-07-02 | 广州市金龟寿药品有限公司 | Walnut kernel polypeptide and preparation method thereof |
Citations (3)
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| CN102406050A (en) * | 2011-11-17 | 2012-04-11 | 中国农业大学 | Walnut low molecular weight polypeptide and preparation method thereof |
| CN102630804A (en) * | 2012-04-27 | 2012-08-15 | 湖北智慧果林业科技有限公司 | Production process of walnut peptide powder |
| CN103125735A (en) * | 2013-03-11 | 2013-06-05 | 武汉工业学院 | Preparation method of walnut polypeptide |
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2013
- 2013-09-06 CN CN2013104017613A patent/CN103468773A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406050A (en) * | 2011-11-17 | 2012-04-11 | 中国农业大学 | Walnut low molecular weight polypeptide and preparation method thereof |
| CN102630804A (en) * | 2012-04-27 | 2012-08-15 | 湖北智慧果林业科技有限公司 | Production process of walnut peptide powder |
| CN103125735A (en) * | 2013-03-11 | 2013-06-05 | 武汉工业学院 | Preparation method of walnut polypeptide |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048417A (en) * | 2017-04-17 | 2017-08-18 | 中南林业科技大学 | A kind of preparation method of the peach kernel polypeptide oral liquor of high bioavilability |
| CN107048417B (en) * | 2017-04-17 | 2020-05-05 | 中南林业科技大学 | Preparation method of peach kernel polypeptide oral liquid with high bioavailability |
| CN107312812A (en) * | 2017-07-11 | 2017-11-03 | 安达市旭朗生物科技有限公司 | A kind of preparation method of ox Cartilage collagen peptide |
| CN108433097A (en) * | 2018-03-02 | 2018-08-24 | 汕头美亨利乐生物科技有限公司 | With the diatery supplement nutriment and preparation method thereof for improving intestinal absorption ability |
| CN109609576A (en) * | 2018-12-29 | 2019-04-12 | 浙江工业大学 | A kind of extracting method of hickory nut polypeptide |
| CN109997944A (en) * | 2019-04-19 | 2019-07-12 | 吉林省东鳌鹿业科技开发有限公司 | A kind of deer tire polypeptide pressed candy and preparation method thereof |
| CN110637879A (en) * | 2019-10-30 | 2020-01-03 | 河北绿岭康维食品有限公司 | Nut roasted seeds and nuts composite antioxidant and method for processing nut roasted seeds and nuts by using same |
| CN113061635A (en) * | 2021-03-31 | 2021-07-02 | 广州市金龟寿药品有限公司 | Walnut kernel polypeptide and preparation method thereof |
| CN113061635B (en) * | 2021-03-31 | 2021-10-08 | 广州市金龟寿药品有限公司 | Walnut kernel polypeptide and preparation method thereof |
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Application publication date: 20131225 |