CN105152690A - Method for preparing protein fertilizer from fish solubles - Google Patents
Method for preparing protein fertilizer from fish solubles Download PDFInfo
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- CN105152690A CN105152690A CN201510483466.6A CN201510483466A CN105152690A CN 105152690 A CN105152690 A CN 105152690A CN 201510483466 A CN201510483466 A CN 201510483466A CN 105152690 A CN105152690 A CN 105152690A
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- fish
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- enzymolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
A method for preparing a protein fertilizer from fish solubles comprises steps as follows: sterilization treatment of the fish solubles, peculiar-smell removal treatment of the fish solubles, preparation of a fish visceral enzyme extracting solution, enzymolysis treatment, drying, smashing and the like. Protease adopted in the process for preparing the protein fertilizer from the fish solubles is extracted from viscera discarded after deep processing of fish, the processing utilization rate of the fish is increased, waste of fish protein resources is reduced, pollution caused by the viscera discarded after deep processing of the fish is reduced, besides, protease in the viscera of the fish is a mixture of various enzymes, protein in the fish solubles can be decomposed in various manners, and the protein in the fish solubles can be completely decomposed.
Description
Technical field
The present invention relates to bio-feritlizer manufacturing process, particularly relate to the method that Protein fertilizer prepared by the molten slurry of a kind of fish.
Background technology
The annual production of domestic fish meal is all more than 300,000 tons, and often produce 1 ton of fish meal, just produce the molten pulp waste of 2-3 ton fish, at present at home, except molten for fish pulp waste is produced except whole fish meal through vacuum concentration by small part enterprise, the enterprise that especially industrial scale is little of major part enterprise, usually by many for molten for these fishes pulp waste direct discharged to ocean, nutritive ingredient wherein and microorganism etc. are had an effect, and rot gradually and produce stench, not only severe contamination ocean environment, but also waste resource.The value of fish molten slurry raw material is cheap, and production method is easy, production power consumption is low, and the various nutritions in raw material are preserved more complete, is the very good material of preparation Protein fertilizer.
Chinese patent notification number CN1260183C, June 21 2006 day for announcing, disclose the preparation method of the biological peptide fertilizer of a kind of fish-protein, it is characterized in that first the tankage of fresh fish, fresh fish skin or fresh fish bone being pulverized, with acid for adjusting pH to 1 ~ 6.5, then add degraded trypsinase or stomach en-degrade, then filter, concentrate at 0 ~ 40 DEG C, sterilizing.The biological peptide fertilizer of fish-protein of this invention contains biological peptide, amino acid whose content is particularly abundant, and containing multiple major element, trace element, VITAMIN etc., this fertilizer can by means of the synergy with other effective constituents, improve disease-resistant, the resistance of plant, can improve the quality of crop, Promoting plant growth, be a kind of novel, environment-friendly type animal proteinum fertilizer.But this kind of Protein fertilizer preparation technology just with the addition of single exogenous protease, a kind of proteolytic enzyme can only be hydrolyzed a kind of specific protein, add the limited efficiency of single proteolytic enzyme protolysate matter, be difficult to thoroughly be hydrolyzed protein all in the molten slurry of fish, affect the effect of Protein fertilizer, and stomach en-needs the effect that just can give full play to protein hydrolysate when pH refers between 1 ~ 3, hydrolysising condition is harsh, is difficult to realize.
Summary of the invention
The present invention to prepare in Protein fertilizer technique existing above-mentioned deficiency to overcome existing Optimization of Low Value Fish, provides a kind ofly to prepare easy, nutritious, crop and soil to be improved to effect molten slurry of fish and prepare Protein fertilizer method.
For achieving the above object, the present invention is by the following technical solutions:
(1) fish molten slurry sterilising treatment: pass into chlorine in the molten slurry of fish, makes chlorine content in the molten slurry of fish remain on the concentration of 1 ~ 5mg/L, sterilising treatment 10 ~ 30 minutes.Chlorine is a kind of sterilant efficiently, just effectively can kill the effect that microorganism in the molten slurry of fish and bacterium play sterilization deactivation under lower concentration.
(2) the molten slurry of fish eliminates the unusual smell process: the molten slurry of the fish after sterilising treatment is complete heats 30 ~ 40 minutes at 60 ~ 80 DEG C of temperature, removes the peculiar smell of fish molten slurry itself and remaining chlorine.The molten slurry of fish processed by fishery product after tankage make and often have denser fishy smell, also having chlorine in previous step after chlorine sterilization remains in the molten slurry of fish, remaining chlorine can affect activity or even the enzyme deactivation of enzyme in follow-up enzymolysis process, affect hydrolysis result, heat can get rid of the remaining chlorine in the molten slurry of fish, also effectively can remove the fishy smell in the molten slurry of fish.
(3) fish viscera zyme extract preparation: the internal organ abandoned after getting fish processing, after cleaning, pulverize under 0 ~ 4 DEG C of environment, adding the mass percentage concentration being pre-chilled to room temperature is 0.5 ~ 1.0%KCl aqueous solution, ultrasonic disperse 20 ~ 30 minutes, homogenate centrifugal treating 10 ~ 15 minutes under rotating speed is 3000 ~ 5000r/min, gets supernatant liquid filtering, obtains fish viscera zyme extract.Proteolytic enzyme of the present invention extracts the internal organ of waste gas after fish deep processing, improves the processing and utilization rate of fish, decreases the waste of fish raw material, the pollution that after decreasing fish deep processing, discarded internal organ cause.In addition, certain proteolytic enzyme that proteolytic enzyme in fish viscera is not single is the mixture of multiple enzyme, the protein in the molten slurry of decomposition fish that can be more various, ensure the thorough decomposition of protein in the molten slurry of fish, the protease hydrolysis condition in fish viscera also relaxes more than pepsic hydrolysising condition.
(4) enzymolysis processing: add obtained fish viscera zyme extract in the molten slurry of the fish after eliminating the unusual smell to sterilizing, the addition of zyme extract is 5 ~ 10% of the molten slurry quality of fish, add the molten slurry of fish after zyme extract 30 ~ 40 DEG C, pH value is enzymolysis processing 5 ~ 10 hours in the gnotobasis of 6.5 ~ 7, go out gained enzymolysis solution at 90 ~ 100 DEG C of temperature after enzymolysis completes ferment treatment 5 ~ 10min.PH value be 6.5 ~ 7 enzymatic hydrolysis condition be the potential of hydrogen being generally applicable to enzyme reaction in fish viscera, and this reaction acid basicity is gentle, be conducive to the life-span ensureing correlated response equipment, carry out going out ferment treatment after enzymolysis completes, after preventing enzymolysis processing complete, proteolytic enzyme continues the polypeptide of enzymolysis gained and acid hydrolysis, ensures the quality of product.
(5) drying and crushing: by the molten slurry crushed after being dried of fish after step (4) process, make Protein fertilizer.
As preferably, the molten slurry of fish eliminate the unusual smell process time can to add addition be that the sorbent material of the molten slurry quality 5 ~ 10% of fish is auxiliary removes peculiar smell and chlorine residue, sorbent material is one or more in diatomite, vermiculite or wilkinite.Fishy smell in the molten slurry of fish is caused by multiple sulfurous organic compound, single heating means possibly fully cannot remove fishy smell, diatomite is a kind of effective sorbent material, effectively can assist and remove peculiar smell, and diatomite, vermiculite and wilkinite safety non-toxic mineral, detrimentally affect can not be caused to enzymolysis process afterwards, also can not affect the fertility of Protein fertilizer.
As preferably, adopt Freeze Drying Technique in step (5), wherein freezing dry process freezing temp is-30 DEG C, and vacuum tightness is 50Pa, and Heating temperature is 60 DEG C, time of drying 5h.Freeze Drying Technique by dry for the molten slurry dewatering and dry of fish after process, ensure that protein wherein can not the too high generation sex change of Yin Wendu and affect the fertility of Protein fertilizer in low temperature environment.
As preferably, enzymolysis solution goes out in ferment treatment temperature-rise period and adopts the temperature rise rate of 15 ~ 20 DEG C/min to be rapidly heated.The enzyme temperature rise rate that goes out fast more effectively can kill the organized enzyme in enzymolysis solution, be enzymolysis also in polypeptide and the material such as amino acid can not be continued to decompose by proteolytic enzyme, ensure the fertility of Protein fertilizer.
Therefore, the present invention has following beneficial effect:
(1) in the molten slurry of fish, breaks down proteins is thorough, is easy to plant absorption;
(2) fish viscera discarded after the present invention uses fish deep processing prepares zyme extract, improves the utilization ratio of fish, the pollution problem of discarded internal organ after also solving fishery product processing.
(3) problem of fish molten slurry the like waste contaminate environment that produces after solving fishery products deep processing of technical scheme of the present invention, for aquatic products processing waste recycling provides an effective way.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
(1) fish molten slurry sterilising treatment: pass into chlorine in the molten slurry of fish, makes chlorine content in the molten slurry of fish remain on the concentration of 1mg/L, sterilising treatment 10 minutes.
(2) the molten slurry of fish eliminates the unusual smell process: the molten slurry of the fish after sterilising treatment is complete heats 30 minutes at 60 DEG C of temperature, adds the diatomite that addition is the molten slurry quality 5% of fish, removes the peculiar smell of fish molten slurry itself and remaining chlorine.
(3) fish viscera zyme extract preparation: internal organ discarded after getting fish processing, after cleaning, pulverize under 0 DEG C of environment, adding the mass percentage concentration being pre-chilled to room temperature is the 0.5%KCl aqueous solution, ultrasonic disperse 20 minutes, homogenate is centrifugal treating 10 clock under rotating speed is 3000r/min, gets supernatant liquid filtering, obtains fish viscera zyme extract.
(4) enzymolysis processing: add obtained fish viscera zyme extract in the molten slurry of the fish after eliminating the unusual smell to sterilizing, the addition of zyme extract is 5% of the molten slurry quality of fish, add the molten slurry of fish after zyme extract 30 DEG C, pH value is enzymolysis processing 5 hours in the gnotobasis of 6.5, go out gained enzymolysis solution at 90 DEG C of temperature after enzymolysis completes ferment treatment 5min, adopts the fast heating ramp rate of 15 DEG C/min in ferment treatment process of going out.
(5) drying and crushing: by through step (4) process after the molten slurry lyophilize of fish after pulverize, make Protein fertilizer, wherein freezing dry process freezing temp is-30 DEG C, and vacuum tightness is 50Pa, and Heating temperature is 60 DEG C, time of drying 5h.
Embodiment 2
(1) fish molten slurry sterilising treatment: pass into chlorine in the molten slurry of fish, makes chlorine content in the molten slurry of fish remain on the concentration of 3mg/L, sterilising treatment 20 minutes.
(2) the molten slurry of fish eliminates the unusual smell process: the molten slurry of the fish after sterilising treatment is complete heats 35 minutes at 70 DEG C of temperature, adds the wilkinite that addition is the molten slurry quality 7.5% of fish, removes the peculiar smell of fish molten slurry itself and remaining chlorine.
(3) fish viscera zyme extract preparation: internal organ discarded after getting fish processing, after cleaning, pulverize under 2 DEG C of environment, adding the mass percentage concentration being pre-chilled to room temperature is the 0.75%KCl aqueous solution, ultrasonic disperse 25 minutes, homogenate centrifugal treating 13 minutes under rotating speed is 4000r/min, gets supernatant liquid filtering, obtains fish viscera zyme extract.
(4) enzymolysis processing: add obtained fish viscera zyme extract in the molten slurry of the fish after eliminating the unusual smell to sterilizing, the addition of zyme extract is 7.5% of the molten slurry quality of fish, add the molten slurry of fish after zyme extract 35 DEG C, pH value is enzymolysis processing 7.5 hours in the gnotobasis of 6.7, go out gained enzymolysis solution at 95 DEG C of temperature after enzymolysis completes ferment treatment 8min, adopts the fast heating ramp rate of 18 DEG C/min in ferment treatment process of going out.
(5) drying and crushing: by through step (4) process after the molten slurry lyophilize of fish after pulverize, make Protein fertilizer, wherein freezing dry process freezing temp is-30 DEG C, and vacuum tightness is 50Pa, and Heating temperature is 60 DEG C, time of drying 5h.
Embodiment 3
(1) fish molten slurry sterilising treatment: pass into a certain amount of chlorine in the molten slurry of fish, makes chlorine content in the molten slurry of fish remain on the concentration of 5mg/L, sterilising treatment 30 minutes.
(2) the molten slurry of fish eliminates the unusual smell process: the molten slurry of the fish after sterilising treatment is complete heats 40 minutes at 80 DEG C of temperature, adds the vermiculite that addition is the molten slurry quality 10% of fish, removes the peculiar smell of fish molten slurry itself and remaining chlorine.
(3) fish viscera zyme extract preparation: internal organ discarded after getting fish processing, after flowing water cleaning, pulverize under 4 DEG C of environment, the mass percentage concentration adding precooling is the 1.0%KCl aqueous solution, extracting 30 minutes, homogenate centrifugal treating 15 minutes under rotating speed is 5000r/min, gets supernatant liquid filtering, obtains fish viscera zyme extract.
(4) enzymolysis processing: add obtained fish viscera zyme extract in the molten slurry of the fish after eliminating the unusual smell to sterilizing, the addition of zyme extract is 10% of the molten slurry quality of fish, add the molten slurry of fish after zyme extract 40 DEG C, pH value is enzymolysis processing 10 hours in the gnotobasis of 7, go out gained enzymolysis solution at 100 DEG C of temperature after enzymolysis completes ferment treatment 10min, adopts the system that is rapidly heated of 20 DEG C/min in ferment treatment process of going out.
(5) drying and crushing: by through step (4) process after the molten slurry lyophilize of fish after pulverize, make Protein fertilizer, wherein freezing dry process freezing temp is-30 DEG C, and vacuum tightness is 50Pa, and Heating temperature is 60 DEG C, time of drying 5h.
Prepared in gained Protein fertilizer conformance with standard NY525-2011 organic fertilizer defined Specifications by the present invention.
Table 1 is by the Related Component detected result of Protein fertilizer obtained in the present invention according to concerned countries standard.
Table 1
| Test item | Unit | Standard-required | Detected result | Detection method |
| Total amino acid content | wt％ | — | 29.553 | GB/T 18246-2000 |
| Moisture | wt％ | ≤20.0 | 2.96 | GB/T 6435-2006 |
| Total nitrogen | wt％ | — | 7.65 | GB/T 17767.1-2008 |
| Total phosphorus | wt％ | — | 3.52 | GB/T 17767.2-2010 |
| Total potassium | wt％ | — | 2.75 | GB/T 17767.3-2010 |
| Organic | wt％ | ≥45.0 | 90.3 | GB/T 18877-2009 |
| Crude fat | wt％ | — | 2.37 | GB/T 6433-2006 |
| Robust fibre | wt％ | — | 2.35 | GB/T 6434-2006 |
Claims (5)
1. a method for Protein fertilizer prepared by the molten slurry of fish, it is characterized in that comprising the following steps:
(1) fish molten slurry sterilising treatment: pass into chlorine in the molten slurry of fish, makes chlorine content in the molten slurry of fish remain on the concentration of 1 ~ 5mg/L, sterilising treatment 10 ~ 30 minutes;
(2) the molten slurry of fish eliminates the unusual smell process: the molten slurry of the fish after sterilising treatment is complete heats 30 ~ 40 minutes at 60 ~ 80 DEG C of temperature;
(3) fish viscera zyme extract preparation: internal organ discarded after getting fish processing, after cleaning, pulverize under 0 ~ 4 DEG C of environment, adding the mass percentage concentration being pre-chilled to room temperature is 0.5 ~ 1.0%KCl aqueous solution, ultrasonic disperse 20 ~ 30 minutes, homogenate centrifugal treating 10 ~ 15 minutes under rotating speed is 3000 ~ 5000r/min, gets supernatant liquid filtering, obtains fish viscera zyme extract;
(4) enzymolysis processing: add obtained fish viscera zyme extract in the molten slurry of the fish after eliminating the unusual smell to sterilizing, the addition of zyme extract is 5 ~ 10% of the molten slurry quality of fish, add the molten slurry of fish after zyme extract 30 ~ 40 DEG C, pH value is enzymolysis processing 5 ~ 10 hours in the gnotobasis of 6.5 ~ 7, go out gained enzymolysis solution at 90 ~ 100 DEG C of temperature after enzymolysis completes ferment treatment 5 ~ 10min;
(5) drying and crushing: by the molten slurry crushed after being dried of fish after step (4) process, make Protein fertilizer.
2. the method for Protein fertilizer prepared by the molten slurry of a kind of fish according to claim 1, it is characterized in that: the molten slurry of described fish eliminates the unusual smell when processing and adds the sorbent material that addition is the molten slurry quality 5 ~ 10% of fish.
3. the method for Protein fertilizer prepared by the molten slurry of a kind of fish according to claim 2, it is characterized in that: described sorbent material is one or more in diatomite, vermiculite or wilkinite.
4. the method for Protein fertilizer prepare by a kind of fish molten slurry according to claim 1, it is characterized in that: employing lyophilize in described step (5), and wherein freezing dry process freezing temp is-30 DEG C, and vacuum tightness is 50Pa, and Heating temperature is 60 DEG C, time of drying 5h.
5. the method for Protein fertilizer prepare by a kind of fish molten slurry according to claim 1, it is characterized in that: the described enzymolysis solution temperature rise rate of employing 15 ~ 20 DEG C/min in ferment treatment temperature-rise period that goes out is rapidly heated.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106747809A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A kind of method that utilization processing of aquatic products accessory substance prepares liquid protein fertilizer |
| CN106748150A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A kind of method of low value ocean fish fermenting and producing amino acid foliage fertilizer |
| CN106810351A (en) * | 2016-12-23 | 2017-06-09 | 浙江海洋大学 | A kind of method that hydrolyzed aquatic products accessory substance prepares amino acid foliage fertilizer |
| CN107056365A (en) * | 2016-12-23 | 2017-08-18 | 浙江海洋大学 | A kind of amino acid foliage fertilizer preparation method planted for organic vegetable |
| CN107089869A (en) * | 2017-06-01 | 2017-08-25 | 慈溪创璞食品科技有限公司 | A kind of preparation method of the molten slurry Protein fertilizer of fish |
| CN109180230A (en) * | 2018-10-18 | 2019-01-11 | 舟山丰宇三立海洋生物肥料有限公司 | A method of less salt high bioactivity organic fertilizer is produced using fish meal machining cooking water |
| FR3123352A1 (en) | 2021-06-01 | 2022-12-02 | Saria | Process for the production of an aqueous solution containing amino acids from a fish soluble(s) - aqueous solution and associated fertilizing products |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106747809A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A kind of method that utilization processing of aquatic products accessory substance prepares liquid protein fertilizer |
| CN106748150A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A kind of method of low value ocean fish fermenting and producing amino acid foliage fertilizer |
| CN106810351A (en) * | 2016-12-23 | 2017-06-09 | 浙江海洋大学 | A kind of method that hydrolyzed aquatic products accessory substance prepares amino acid foliage fertilizer |
| CN107056365A (en) * | 2016-12-23 | 2017-08-18 | 浙江海洋大学 | A kind of amino acid foliage fertilizer preparation method planted for organic vegetable |
| CN107089869A (en) * | 2017-06-01 | 2017-08-25 | 慈溪创璞食品科技有限公司 | A kind of preparation method of the molten slurry Protein fertilizer of fish |
| CN109180230A (en) * | 2018-10-18 | 2019-01-11 | 舟山丰宇三立海洋生物肥料有限公司 | A method of less salt high bioactivity organic fertilizer is produced using fish meal machining cooking water |
| FR3123352A1 (en) | 2021-06-01 | 2022-12-02 | Saria | Process for the production of an aqueous solution containing amino acids from a fish soluble(s) - aqueous solution and associated fertilizing products |
| WO2022254147A1 (en) | 2021-06-01 | 2022-12-08 | Saria | Method for producing an aqueous solution containing amino acids from fish soluble matter- aqueous solution and associated fertilising products |
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Application publication date: 20151216 |