CN105060944A - Method for preparing egg white fertilizer by fish soluble - Google Patents

Method for preparing egg white fertilizer by fish soluble Download PDF

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Publication number
CN105060944A
CN105060944A CN201510483682.0A CN201510483682A CN105060944A CN 105060944 A CN105060944 A CN 105060944A CN 201510483682 A CN201510483682 A CN 201510483682A CN 105060944 A CN105060944 A CN 105060944A
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China
Prior art keywords
fish
molten slurry
substratum
molten
slurry
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CN201510483682.0A
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Chinese (zh)
Inventor
欧阳小琨
杨立业
金如娜
郁迪
徐银峰
卢娇
王阳光
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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Priority to CN201510483682.0A priority Critical patent/CN105060944A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Abstract

The invention relates to a method for preparing an egg white fertilizer by fish soluble. The method comprises the steps: fish soluble sterilization treatment; fish soluble odor-eliminating treatment; culture medium preparation; strain culture; fermentation treatment; drying and crushing and the like. The strain for fermentation is a JT strain which comprises various strains capable of generating protease, for example, bacillus subtilis, trichoderma sp, saccharomycetes and the like as well as various strains which play an improving role on soil, for example, nitrogen-fixing bacteria, bacillus siliceous, photosynthetic bacteria and the like. The egg white fertilizer prepared by the method not only plays roles of providing nutrients and increasing production for crops, but also can be used for improving the soil condition and promoting healthy growth of the crops.

Description

The method of Protein fertilizer prepared by the molten slurry of a kind of fish
Technical field
The present invention relates to bio-feritlizer manufacturing process, particularly relate to the method that Protein fertilizer prepared by the molten slurry of a kind of fish.
Background technology
The annual production of domestic fish meal is all more than 300,000 tons, and often produce 1 ton of fish meal, just produce the molten pulp waste of 2-3 ton fish, at present at home, except molten for fish pulp waste is produced except whole fish meal through vacuum concentration by small part enterprise, the enterprise that especially industrial scale is little of major part enterprise, often by many for molten for these fishes pulp waste direct discharged to ocean, nutritive ingredient wherein and environmental microorganism etc. are had an effect, and rot gradually and produce stench, not only severe contamination ocean environment, but also waste resource.Fish molten slurry raw material is worth cheap, and raw material sources are extensive, production power consumption is low, and the various nutritions in raw material are preserved more complete, is the very good material of preparation Protein fertilizer.
Chinese patent notification number CN1260183C, June 21 2006 day for announcing, disclose the preparation method of the biological peptide fertilizer of a kind of fish-protein, it is characterized in that first the tankage of fresh fish, fresh fish skin or fresh fish bone being pulverized, with acid for adjusting pH to 1 ~ 6.5, then add degraded trypsinase or stomach en-degrade, then filter, concentrate at 0 ~ 40 DEG C, sterilizing.The biological peptide fertilizer of fish-protein of this invention contains biological peptide, amino acid whose content is particularly abundant, and containing multiple major element, trace element, VITAMIN etc., this fertilizer can by means of the synergy with other effective constituents, improve disease-resistant, the resistance of plant, can improve the quality of crop, Promoting plant growth, be a kind of novel, environment-friendly type animal proteinum fertilizer.But this kind of Protein fertilizer preparation technology only with the addition of the protein in single protease hydrolysis fish pomace, limited to the improvement result of obtained Protein fertilizer quality, and the application effect of raising Protein fertilizer not yet in effect.
Summary of the invention
The present invention to prepare in Protein fertilizer technique existing above-mentioned deficiency to overcome existing Optimization of Low Value Fish, provides a kind ofly to prepare easy, nutritious, crop and soil to be improved to effect molten slurry of fish and prepare Protein fertilizer method.
For achieving the above object, the present invention is by the following technical solutions:
A method for Protein fertilizer prepared by the molten slurry of fish, comprises the following steps:
(1) fish molten slurry sterilising treatment: pass into ozone to the molten slurry of fish, makes ozone concn in the molten slurry of fish remain on 8 ~ 12mg/m 3, sterilising treatment 10 ~ 20 minutes.Ozone is a kind of sterilant efficiently, just effectively can kill the effect that microorganism in the molten slurry of fish and bacterium play sterilization deactivation under lower concentration.
(2) the molten slurry of fish removes peculiar odor treatment: the molten slurry of the fish after sterilising treatment is complete heats 30 ~ 40 minutes at 60 ~ 80 DEG C of temperature, the peculiar smell of removing fish molten slurry itself and residual ozone.The molten slurry of fish processed by fishery product after tankage make and often have denser fishy smell, also having ozone in previous step after ozone sterilization remains in the molten slurry of fish, heat can get rid of the residual ozone in the molten slurry of fish, also effectively can remove the fishy smell in the molten slurry of fish.
(3) preparation of substratum: substratum is made up of the component of following mass percent: sodium-chlor 0.5 ~ 1.0%, potassium primary phosphate 0.3 ~ 0.5%, magnesium sulfate 0.05 ~ 0.1%, agar 5 ~ 10%, glucose 10 ~ 15%, peptone 5 ~ 8%, starch 10 ~ 20%, surplus is water, first after the water furnishing pasty state of starch half, pour residue water into, sodium-chlor is added successively after stirring evenly, potassium primary phosphate, magnesium sulfate, agar, glucose, peptone, make them dissolve, mixed solution is heated to after boiling and adds agar, do not stop to stir, after agar dissolves completely, adjust ph, sterilizing after packing.The microorganism inoculant adopted in this programme contains multiple-microorganism, and its growth medium composition is slightly different, and therefore this microorganism inoculant substratum comprises the nutritive substance needed for multiple-microorganism growth and breeding.
(4) spawn culture: the microorganism inoculant of getting substratum quality 10 ~ 20%, microorganism inoculant is seeded on substratum, pass into sterile air, cultivate 2 ~ 4 days under 30 ~ 35 DEG C of culture temperature, after cultivation, microorganism is pulverized together with substratum and make.The cultivation of bacterial classification, can expand the quantity of microorganism, can reduce production cost to a certain extent.
(5) fermentative processing: by cultivate after microbial bacteria by fish molten slurry quality 5 ~ 8% amount add in the molten slurry of fish, fermentative processing 6 ~ 8 days.
(6) drying and crushing: make Protein fertilizer by after molten for the fish after fermentative processing slurry drying and crushing.
As preferably, the molten slurry of described fish eliminate the unusual smell process time can add that the diatomite powder of the molten slurry quality 5 ~ 10% of fish is auxiliary removes peculiar smell.Fishy smell in the molten slurry of fish is caused by multiple sulfurous organic compound, single heating means possibly fully cannot remove fishy smell, diatomite is a kind of effective sorbent material, effectively can assist and remove peculiar smell, and diatomite safety non-toxic, detrimentally affect can not be caused to microbial cultivation process afterwards, also can not affect the fertility of Protein fertilizer.
As preferably, described microorganism inoculant is JT bacteria preparation.JT bacteria preparation contains bacterial classification in actinomycetes, vinelandii, silicate bacteria, subtilis, Paenibacillus polymyxa, Trichoderma, yeast and photosynthetic bacteria etc. 8, wherein actinomycetes, subtilis, Trichoderma and yeast have the function of decomposing protein protein fully can be resolved into polypeptide, amino acid etc., are conducive to fertilising ensuing crop to the absorption of Protein fertilizer.JT bacteria preparation inherently a kind of microbial preparation being widely used in soil improvement, suppressing soil germ, promote fruits and vegetables growth, can make multiple beneficial microorganism form dominant colony in the breeding of soil high speed in addition.Effective elimination soil germ, degraded fertilizer damage, decompose the organic substance in soil, improve phosphorus, potassium, alkali-hydrolyzable nitrogen content, alleviate soil compaction situation, improve its water conservation, ventilation property, the metabolic capabilities of crop can be strengthened, promote well developed root system, and impel the large fruit of melon-fruit-like vegetable melon many, color and luster profit is gorgeous, quality improving; Accelerate it to germinate, bloom and maturation time.
As preferably, the starch added in described substratum is one or more in W-Gum, sweet potato starch or yam starch.
As preferably, the sterilising treatment of described substratum adopts high pressure steam sterilization process.
Therefore, the present invention has following beneficial effect:
(1) in the molten slurry of fish, breaks down proteins is thorough, is easy to plant absorption;
(2) adopt crop and the useful fermentable of soil, not only effective is that polypeptide and amino acid are conducive to plant growth by breaks down proteins, can also improve soil, the germ suppressed in soil, is more conducive to crop yield and increases income;
(3) problem of fish molten slurry the like waste contaminate environment that produces after solving fishery products deep processing of technical scheme of the present invention, for aquatic products processing waste recycling provides an effective way.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
(1) fish molten slurry sterilising treatment: pass into ozone to the molten slurry of fish, makes ozone concn in the molten slurry of fish remain on 8mg/m 3, sterilising treatment 10 minutes.
(2) the molten slurry of fish removes peculiar odor treatment: the molten slurry of the fish after sterilising treatment is complete heats 30 minutes at 60 DEG C of temperature, the peculiar smell of removing fish molten slurry itself and residual ozone.
(3) preparation of substratum: substratum is made up of the component of following mass percent: sodium-chlor 0.5%, potassium primary phosphate 0.3%, magnesium sulfate 0.05%, agar 5%, glucose 10%, peptone 8%, starch 20%, water 56.15%, first after the water furnishing pasty state of starch half, pour residue water into, after stirring evenly, add sodium-chlor, potassium primary phosphate, magnesium sulfate, agar, glucose, peptone, make them dissolve, mixed solution is heated to after boiling and adds agar, do not stop to stir, after agar dissolves completely, regulate pH, after packing, use steam sterilizing.
(4) spawn culture: the microorganism inoculant of getting substratum quality 10%, is seeded in microorganism inoculant on substratum, passes into sterile air, cultivates 2 days, pulverized by microorganism and make after cultivation together with substratum under 30 DEG C of culture temperature.
(5) fermentative processing: the microbial bacteria after cultivating is added in the molten slurry of fish by the amount of fish molten slurry quality 5%, fermentative processing 6 days.
(6) drying and crushing: make Protein fertilizer by after molten for the fish after fermentative processing slurry drying and crushing.
The molten slurry of fish in the present embodiment processed by fish meal after waste liquid through concentrated obtained.
Embodiment 2
(1) fish molten slurry sterilising treatment: pass into a certain amount of ozone to the molten slurry of fish, makes ozone concn in the molten slurry of fish remain on 10mg/m 3, sterilising treatment 15 minutes.
(2) the molten slurry of fish removes peculiar odor treatment: the molten slurry of the fish after sterilising treatment is complete heats 35 minutes at 70 DEG C of temperature, the peculiar smell of removing fish molten slurry itself and residual ozone.
(3) preparation of substratum: substratum is made up of the component of following mass percent: sodium-chlor 0.75%, potassium primary phosphate 0.4%, magnesium sulfate 0.075%, agar 7.5%, glucose 12.5%, peptone 6.5%, starch 15%, water 57.275%, first after the water furnishing pasty state of starch half, pour residue water into, sodium-chlor is added after stirring evenly, potassium primary phosphate, magnesium sulfate, agar, glucose, peptone, make its dissolve, mixed solution is heated to after boiling and adds agar, do not stop to stir, after agar dissolves completely, regulate pH, steam sterilizing is used after packing.
(4) spawn culture: the microorganism inoculant of getting substratum quality 15%, is seeded in microorganism inoculant on substratum, passes into sterile air, cultivates 3 days, pulverized by microorganism and make after cultivation together with substratum under 33 DEG C of culture temperature.
(5) fermentative processing: the microbial bacteria after cultivating is added in the molten slurry of fish by the amount of fish molten slurry quality 6.5%, fermentative processing 7 days.
(6) drying and crushing: make Protein fertilizer by after molten for the fish after fermentative processing slurry drying and crushing.
The molten slurry of fish in the present embodiment processed by fish meal after waste liquid through concentrated obtained.
Embodiment 3
(1) fish molten slurry sterilising treatment: pass into a certain amount of ozone to the molten slurry of fish, makes ozone concn in the molten slurry of fish remain on 12mg/m 3, sterilising treatment 20 minutes.
(2) the molten slurry of fish removes peculiar odor treatment: the molten slurry of the fish after sterilising treatment is complete heats 40 minutes at 80 DEG C of temperature, the peculiar smell of removing fish molten slurry itself and residual ozone.
(3) preparation of substratum: substratum is made up of the component of following mass percent: sodium-chlor 1.0%, potassium primary phosphate 0.5%, magnesium sulfate 0.1%, agar 10%, glucose 15%, peptone 5%, starch 10%, water 58.4%, first after the water furnishing pasty state of starch half, pour residue water into, after stirring evenly, add sodium-chlor, potassium primary phosphate, magnesium sulfate, agar, glucose, peptone, make them dissolve, mixed solution is heated to after boiling and adds agar, do not stop to stir, after agar dissolves completely, regulate pH, after packing, use steam sterilizing.
(4) spawn culture: the microorganism inoculant of getting substratum quality 20%, is seeded in microorganism inoculant on substratum, passes into sterile air, cultivates 4 days, pulverized by microorganism and make after cultivation together with substratum under 35 DEG C of culture temperature.
(5) fermentative processing: the microbial bacteria after cultivating is added in the molten slurry of fish by the amount of fish molten slurry quality 8%, fermentative processing 8 days.
(6) drying and crushing: make Protein fertilizer by after molten for the fish after fermentative processing slurry drying and crushing.
The molten slurry of fish in the present embodiment processed by fish meal after waste liquid through concentrated obtained.
Following technical indicator can be realized by the present invention:
1. the Protein fertilizer preparing gained by the present invention also have improve soil, the germ suppressed in soil, be more conducive to crop yield and increase income;
2. the Protein fertilizer preparing gained by the present invention meets in agricultural industry criteria NY525-2011 the organism technological standard of organic fertilizer defined and the standard-required of pathogenic bacterium.Table 1 is by the Related Component detected result of Protein fertilizer obtained in the present invention according to concerned countries standard.
Table 1
Test item Unit Standard-required Detected result Detection method
Total amino acid content wt% 29.49 GB/T 18246-2000
Moisture wt% ≤20.0 2.46 GB/T 6435-2006
Total nitrogen wt% 7.65 GB/T 17767.1-2008
Total phosphorus wt% 3.52 GB/T 17767.2-2010
Total potassium wt% 2.75 GB/T 17767.3-2010
Organic wt% ≥45.0 90.3 GB/T 18877-2009
Crude fat wt% 2.82 GB/T 6433-2006
Robust fibre wt% 2.35 GB/T 6434-2006

Claims (5)

1. a method for Protein fertilizer prepared by the molten slurry of fish, it is characterized in that the method comprises the following steps:
(1) fish molten slurry sterilising treatment: pass into ozone to the molten slurry of fish, makes ozone concn in the molten slurry of fish remain on 8 ~ 12mg/m3, sterilising treatment 10 ~ 20 minutes;
(2) the molten slurry of fish removes peculiar odor treatment: the molten slurry of the fish after sterilising treatment is complete heats 30 ~ 40 minutes at 60 ~ 80 DEG C of temperature;
(3) preparation of substratum: substratum is made up of the component of following mass percent: sodium-chlor 0.5 ~ 1.0%, potassium primary phosphate 0.3 ~ 0.5%, magnesium sulfate 0.05 ~ 0.1%, agar 5 ~ 10%, glucose 10 ~ 15%, peptone 5 ~ 8%, starch 10 ~ 20%, surplus is water;
(4) spawn culture: the microorganism inoculant of getting substratum quality 10 ~ 20%, microorganism inoculant is seeded on substratum, pass into sterile air, cultivate 2 ~ 4 days under 30 ~ 35 DEG C of culture temperature, after cultivation, microorganism is pulverized together with substratum and make microbial mixture;
(5) fermentative processing: by microbial mixture by fish molten slurry quality 5 ~ 8% amount add in the molten slurry of fish, fermentative processing 6 ~ 8 days;
(6) drying and crushing: make Protein fertilizer by after molten for the fish after fermentative processing slurry drying and crushing.
2. the method for Protein fertilizer prepared by the molten slurry of a kind of fish according to claim 1, it is characterized in that: the molten slurry of described fish eliminates the unusual smell and adds the diatomite powder of fish molten slurry quality 5 ~ 10% when processing.
3. the method for Protein fertilizer prepared by the molten slurry of a kind of fish according to claim 1, it is characterized in that: described microorganism inoculant is JT bacteria preparation.
4. the method for Protein fertilizer prepared by the molten slurry of a kind of fish according to claim 1, it is characterized in that: the starch added in described substratum is one or more in W-Gum, sweet potato starch or yam starch.
5. the method for Protein fertilizer prepared by the molten slurry of a kind of fish according to claim 1, it is characterized in that: the sterilising treatment of described substratum adopts steam sterilizing process.
CN201510483682.0A 2015-08-07 2015-08-07 Method for preparing egg white fertilizer by fish soluble Pending CN105060944A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105541518A (en) * 2016-01-14 2016-05-04 连云港市耕地质量保护站 Ecological special fertilizer of common yam rhizome and preparation and application method of ecological special fertilizer
CN105695546A (en) * 2016-03-09 2016-06-22 广东海洋大学 Method for preparing liquid organic fertilizer through fermentation of aquatic product waste
CN107337505A (en) * 2017-06-14 2017-11-10 芜湖县花桥镇恒乐水果种植专业合作社 A kind of plum plantation high nitrogen fertilizer material
CN108902466A (en) * 2018-07-18 2018-11-30 岭商(浙江)生物科技有限公司 A kind of molten slurry of hydrolysis fish and its production method and application
CN109180230A (en) * 2018-10-18 2019-01-11 舟山丰宇三立海洋生物肥料有限公司 A method of less salt high bioactivity organic fertilizer is produced using fish meal machining cooking water

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CN102992905A (en) * 2012-12-21 2013-03-27 潘少波 Fermentation liquid fertilizer suitable for drop irrigation of greenhouse crops
CN104557339A (en) * 2015-02-03 2015-04-29 舟山源丰海洋生物科技有限公司 Preparation method of soluble fish protein ammonia acid fertilizer

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CN102219563A (en) * 2011-03-15 2011-10-19 武汉市绿丹茂科技有限责任公司 Process for preparing biological organic fertilizer from fish
CN102992905A (en) * 2012-12-21 2013-03-27 潘少波 Fermentation liquid fertilizer suitable for drop irrigation of greenhouse crops
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105541518A (en) * 2016-01-14 2016-05-04 连云港市耕地质量保护站 Ecological special fertilizer of common yam rhizome and preparation and application method of ecological special fertilizer
CN105695546A (en) * 2016-03-09 2016-06-22 广东海洋大学 Method for preparing liquid organic fertilizer through fermentation of aquatic product waste
CN107337505A (en) * 2017-06-14 2017-11-10 芜湖县花桥镇恒乐水果种植专业合作社 A kind of plum plantation high nitrogen fertilizer material
CN108902466A (en) * 2018-07-18 2018-11-30 岭商(浙江)生物科技有限公司 A kind of molten slurry of hydrolysis fish and its production method and application
CN109180230A (en) * 2018-10-18 2019-01-11 舟山丰宇三立海洋生物肥料有限公司 A method of less salt high bioactivity organic fertilizer is produced using fish meal machining cooking water

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Application publication date: 20151118