CN104212863A - Preparation method of feed-use animal protein peptide - Google Patents
Preparation method of feed-use animal protein peptide Download PDFInfo
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- CN104212863A CN104212863A CN201410365237.XA CN201410365237A CN104212863A CN 104212863 A CN104212863 A CN 104212863A CN 201410365237 A CN201410365237 A CN 201410365237A CN 104212863 A CN104212863 A CN 104212863A
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- Prior art keywords
- feather
- protein peptide
- feed
- animal protein
- preparation
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 15
- 235000021120 animal protein Nutrition 0.000 title claims abstract description 9
- 238000002360 preparation method Methods 0.000 title abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims abstract description 5
- 230000004151 fermentation Effects 0.000 claims abstract description 5
- 210000003746 feather Anatomy 0.000 claims description 31
- 235000012054 meals Nutrition 0.000 claims description 13
- 241000194103 Bacillus pumilus Species 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 abstract description 9
- 235000018102 proteins Nutrition 0.000 abstract description 8
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000003674 animal food additive Substances 0.000 abstract description 2
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 229920002521 macromolecule Polymers 0.000 abstract description 2
- 235000019629 palatability Nutrition 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000001007 puffing effect Effects 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 108091005658 Basic proteases Proteins 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000036039 immunity Effects 0.000 abstract 1
- 108010059345 keratinase Proteins 0.000 abstract 1
- 230000035790 physiological processes and functions Effects 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 230000009102 absorption Effects 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Fodder In General (AREA)
Abstract
A preparation method of a feed-use animal protein peptide. The invention belongs to the technical field of feed additives and particularly relates to the preparation method of the feed-use animal protein peptide. The method includes following steps: performing an impurity-removing process, a washing process and a drying process to furs; puffing the furs; performing enzymolysis with keratinase and alkaline protease and performing fermentation with bacillus pumillus; performing centrifugation to obtain a supernate; and concentrating and spray-drying the supernate to obtain a finished product. The feed-use animal protein peptide is prepared with furs as a raw material. In a fur powder, macromolecular substances, such as proteins and the like, are degraded into micromolecular substances, such as protein peptide and the like. The method integrates advantages of the puffing method, the enzymic method and the fermenting method effectively, not only is high in preparation speed, free of bitter taste and odor and strong in palatability, but also has various physiological functions of promoting growth of animals, enhancing body immunity of animals and the like.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of making method of feeding animal protein peptide.
Background technology
In feather, protein content reaches more than 80%, amino acid profiles is more complete, except Methionin, methionine(Met) are lower than except fish meal, all the other indispensable amino acids are all higher than fish meal, and the content of Gelucystine, shelter has first of natural feed, be good protein feed resource, but in feather, the albumen of 90% is constitutionally stable Keratin sulfate, in feed, adds being difficult to by the direct absorption and digestion of animal, not only cause the waste of protein resource, also can cause serious environmental pollution.
If by protein degradation in feather be feeding animals again after small-molecule peptide, so not only utilization ratio can improve greatly, and alleviating the ablactation stress of youngling, reduce diarrhoea, the aspects such as growth promoting effects and raising animal organism immunizing power all have good effect, that is by biochemical technology, macromolecular weight protein in feather is degraded into feather peptide, the using value of protein source can be significantly improved, feather peptide can directly be absorbed by animal body digestive tube, there is transport speed fast, consume energy the low and not easily feature such as saturated, avoid occurring competing with the absorption of total free aminoacids, substantially increase the absorption rate of albumen, in addition feather peptide also has promotion calcium, the mineral absorptions such as iron, strengthen the effects such as the breeding of the profitable strain in animal digestive tract.
Summary of the invention
The object of the present invention is to provide a kind of take feather as the method that raw material makes feeding animal protein peptide.
The object of the present invention is achieved like this:
Feather is through removal of impurities, moisture 18-25% is dried to after cleaning, expanded with feather expander, feather bulking thing after expanded is pulverized and crossed 100 mesh sieve and namely obtains feather meal, take the feather meal of certain mass, the water adding 8-10 times of quality stirs, PH is adjusted to 8-8.5, the ratio adding 30-50 unit in every gram of feather meal adds M-Zyme, enzymolysis 1 hour under 50-55 DEG C of condition, the ratio adding 20-30 unit in every gram of feather meal again adds Sumizyme MP, enzymolysis 2-3 hour again, 121 DEG C of enzyme 15-20 minute that go out, cool to 28-30 DEG C, by the inoculum size inoculation bacillus pumilus seed liquor of 5-10%, at 120-160rpm, the shaking table top fermentation 30-36 hour of 28-30 DEG C, centrifugal clear liquid, clear liquid is again through concentrated, spraying dry gets product.
The present invention also comprises following feature:
The preparation method of described bacillus pumilus seed liquor is: take peptone 5g, extractum carnis 3g, sodium-chlor 5g, add water and be settled to 1000 milliliters, PH is adjusted to 7.0-7.2, and 121 DEG C of sterilizing 15-20 minute, are cooled to 28-30 DEG C, inoculation bacillus pumilus, 18-20 hour cultivated by the shaking table of 120-160rpm, 28-30 DEG C.
Feeding animal protein peptide of the present invention take feather as raw material, successively by expanded, enzymolysis and fermentative action, the macromolecular substance such as the protein in feather meal are degraded into the small-molecule substances such as protein peptide, effectively combine expanded method, enzyme process and fermentation method separately advantage, not only prepare speed fast, without bitter taste and peculiar smell, palatability is strong, and has the different physiological roles such as promotion animal growth, raising animal body immunizing power.
Specific embodiments
The bacterial strain bacillus pumilus CICC20075 that the present invention adopts purchases in Chinese industrial Microbiological Culture Collection administrative center.
Take peptone 5g, extractum carnis 3g, sodium-chlor 5g, add water and be settled to 1000 milliliters, PH is adjusted to 7.2,121 DEG C of sterilizings 15 minutes, be cooled to 28 DEG C, inoculation bacillus pumilus, 150rpm, the shaking table of 28 DEG C cultivated and within 20 hours, namely obtains bacillus pumilus seed liquor.
Feather is through removal of impurities, moisture 20% is dried to after cleaning, expanded with feather expander, feather bulking thing after expanded is pulverized and crossed 100 mesh sieve and namely obtains feather meal, take the feather meal of certain mass, the water adding 10 times of quality stirs, PH is adjusted to 8, the ratio adding 40 units in every gram of feather meal adds M-Zyme, enzymolysis 1 hour under 55 DEG C of conditions, the ratio adding 25 units in every gram of feather meal again adds Sumizyme MP, enzymolysis 3 hours again, 121 DEG C of enzymes 15 minutes of going out, cool to 28 DEG C, by the inoculum size inoculation bacillus pumilus seed liquor of 10%, at 150rpm, the shaking table top fermentation of 28 DEG C 33 hours, centrifugal clear liquid, clear liquid is again through concentrated, spraying dry gets product.
Claims (1)
1. a making method for feeding animal protein peptide, is characterized in that being prepared from through the following course of processing:
(1) take peptone 5g, extractum carnis 3g, sodium-chlor 5g, add water and be settled to 1000 milliliters, PH is adjusted to 7.0-7.2, and 121 DEG C of sterilizing 15-20 minute, are cooled to 28-30 DEG C, inoculation bacillus pumilus, the shaking table of 120-160rpm, 28-30 DEG C is cultivated and within 18-20 hour, namely obtains bacillus pumilus seed liquor;
(2) feather is through removal of impurities, moisture 18-25% is dried to after cleaning, expanded with feather expander, feather bulking thing after expanded is pulverized and crossed 100 mesh sieve and namely obtains feather meal, take the feather meal of certain mass, the water adding 8-10 times of quality stirs, PH is adjusted to 8-8.5, the ratio adding 30-50 unit in every gram of feather meal adds M-Zyme, enzymolysis 1 hour under 50-55 DEG C of condition, the ratio adding 20-30 unit in every gram of feather meal again adds Sumizyme MP, enzymolysis 2-3 hour again, 121 DEG C of enzyme 15-20 minute that go out, cool to 28-30 DEG C, by the inoculum size inoculation bacillus pumilus seed liquor of 5-10%, at 120-160rpm, the shaking table top fermentation 30-36 hour of 28-30 DEG C, centrifugal clear liquid, clear liquid is again through concentrated, spraying dry gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410365237.XA CN104212863A (en) | 2014-07-29 | 2014-07-29 | Preparation method of feed-use animal protein peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410365237.XA CN104212863A (en) | 2014-07-29 | 2014-07-29 | Preparation method of feed-use animal protein peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104212863A true CN104212863A (en) | 2014-12-17 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410365237.XA Pending CN104212863A (en) | 2014-07-29 | 2014-07-29 | Preparation method of feed-use animal protein peptide |
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| CN (1) | CN104212863A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798981A (en) * | 2015-03-17 | 2015-07-29 | 齐鲁工业大学 | Method for preparing feather protein powder from alkaline protease/keratinase |
| CN105175161A (en) * | 2015-10-22 | 2015-12-23 | 浙江省农业科学院 | Composite amino acid liquid fertilizer and preparation method thereof |
| CN105293599A (en) * | 2015-10-10 | 2016-02-03 | 北海富安源生物科技有限公司 | Method for increasing synthetic ratio in hair hydrolyzation for preparing aquatic ecological protecting agent |
| CN105494886A (en) * | 2015-12-03 | 2016-04-20 | 王金光 | Hydrolyzed protein powder and preparation method thereof |
| CN107173521A (en) * | 2016-03-09 | 2017-09-19 | 广东海洋大学 | A kind of preparation method of ferment feather powder |
| CN107173534A (en) * | 2016-03-09 | 2017-09-19 | 广东海洋大学 | A kind of utilization aquatic product protein and feather meal fermentation prepare the method that fish meal substitutes albumen |
| CN108497157A (en) * | 2018-04-09 | 2018-09-07 | 广东希普生物科技股份有限公司 | High digestibility enzymolysis feather powder and preparation method thereof |
| CN108835387A (en) * | 2018-06-15 | 2018-11-20 | 安徽皓越生物科技有限公司 | A kind of preparation method of functionality glycolysis feather meal feed |
| CN112655825A (en) * | 2020-12-23 | 2021-04-16 | 安徽希普生物科技有限公司 | Feather protein peptide particle composition and preparation method thereof |
| CN114653434A (en) * | 2022-03-19 | 2022-06-24 | 江苏新瑞药业有限公司 | Mixing method for preparing polypeptide complex and application |
| CN115161368A (en) * | 2022-06-28 | 2022-10-11 | 广东省科学院生物与医学工程研究所 | Method for directly and biologically converting waste feather into feed protein powder |
-
2014
- 2014-07-29 CN CN201410365237.XA patent/CN104212863A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798981A (en) * | 2015-03-17 | 2015-07-29 | 齐鲁工业大学 | Method for preparing feather protein powder from alkaline protease/keratinase |
| CN105293599A (en) * | 2015-10-10 | 2016-02-03 | 北海富安源生物科技有限公司 | Method for increasing synthetic ratio in hair hydrolyzation for preparing aquatic ecological protecting agent |
| CN105175161A (en) * | 2015-10-22 | 2015-12-23 | 浙江省农业科学院 | Composite amino acid liquid fertilizer and preparation method thereof |
| CN105494886A (en) * | 2015-12-03 | 2016-04-20 | 王金光 | Hydrolyzed protein powder and preparation method thereof |
| CN107173521A (en) * | 2016-03-09 | 2017-09-19 | 广东海洋大学 | A kind of preparation method of ferment feather powder |
| CN107173534A (en) * | 2016-03-09 | 2017-09-19 | 广东海洋大学 | A kind of utilization aquatic product protein and feather meal fermentation prepare the method that fish meal substitutes albumen |
| CN108497157A (en) * | 2018-04-09 | 2018-09-07 | 广东希普生物科技股份有限公司 | High digestibility enzymolysis feather powder and preparation method thereof |
| CN108835387A (en) * | 2018-06-15 | 2018-11-20 | 安徽皓越生物科技有限公司 | A kind of preparation method of functionality glycolysis feather meal feed |
| CN112655825A (en) * | 2020-12-23 | 2021-04-16 | 安徽希普生物科技有限公司 | Feather protein peptide particle composition and preparation method thereof |
| CN114653434A (en) * | 2022-03-19 | 2022-06-24 | 江苏新瑞药业有限公司 | Mixing method for preparing polypeptide complex and application |
| CN114653434B (en) * | 2022-03-19 | 2024-01-02 | 江苏新瑞药业有限公司 | Mixing method for preparing polypeptide complex |
| CN115161368A (en) * | 2022-06-28 | 2022-10-11 | 广东省科学院生物与医学工程研究所 | Method for directly and biologically converting waste feather into feed protein powder |
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Application publication date: 20141217 |