CN103931982B - A kind of supersonic synergic impulse electric field prepares the method for pollen nutrient liquid - Google Patents
A kind of supersonic synergic impulse electric field prepares the method for pollen nutrient liquid Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of method that supersonic synergic impulse electric field prepares pollen nutrient liquid, comprise the following steps: (1) will go deimpurity pollen to add in Adlerika, obtain pollen solution after mixing; (2) ultrasonic pre-treatment is carried out to pollen solution; (3) high-pressure pulse electric process is carried out to the pollen solution after step (2) process, obtain pollen nutrient liquid.The rate of breaking pollen wall that the present invention obtains in pollen nutrient liquid is high, and technique is simple, not through high-temperature process in leaching process, reduces the destruction of the nutriment in pollen.
Description
Technical field
The present invention relates to the preparation method of pollen nutrient liquid, particularly a kind of supersonic synergic impulse electric field prepares the method for pollen nutrient liquid.
Background technology
Bee Pollen is the dough that the pollen gathering the sources of nectar and pollen by honeybee is formed through processing, the nutritional labeling of Bee Pollen is very abundant, not only containing rich in protein, carbohydrate, lipid, vitamin, organic acid, mineral matter, nucleic acid, also containing enzyme and other biological active material.Bee Pollen all has certain therapeutic action to cardiovascular and cerebrovascular disease, prostate cancer, hyperplasia of prostate, hepatopathy, habitual constipation, anaemia, diabetes, asthma etc.In addition, also have antifatigue, strengthen muscle power, strengthen memory, improve brain function, strengthen hypoxia-bearing capability, regulate the effects such as endocrine, improvement function and anti-ageing life-prolonging beauty treatment.But because pollen has solid cell membrane (the isopyknic material composition of sporopollenin, pectic substance, cellulose), can the degradation of withstand high pressures, high temperature, acid and alkali corrosion and enzyme, therefore be difficult to be digested and assimilated by human gastrointestinal tract, have impact on the human body utilization rate of the nutritional labeling of pollen.People's researchs such as Bell show, non-broken pollen is by digestion, and the digestibility of pollen particles is only 52%-59%; The people such as Zhang Hongcheng study and find to increase to some extent compared to not broken wall tea flower pollen its non-oxidizability after enzymolysis; The people such as Fan Bailin have studied the blood fat reducing function of broken pollen, effectively can reduce the blood fat in Hyperlipemia population serum after finding pollen pini broken wall; After the research of the people such as Zhao Lin then shows pollen pini broken wall, crude fibre, hemicellulose and content of lignin significantly reduce, and water-soluble and liposoluble constituent stripping quantity then greatly improves; The people such as Sun Liping have studied the broken wall effect of supercritical carbon dioxide to rape pollen, find that the fatty acid species in the pollen opened through germinal aperature increases; The people such as Ruan Zheng have studied the broken wall effect of multiple method to rape pollen, find that the flavones content detected of the pollen after process increases.Therefore broken wall treatment is carried out to pollen, not only can strengthen human body to the utilization of pollens nutrition material but also contribute to developing more pollen product.
Be applied to method mainly difference Poluo wall method, colloid mill broken wall method, air-flow broken wall method and the biological fermentation process etc. of pollen broken wall at present.Chinese patent CN96117168.5 discloses a kind of pollen wall-breaking technology, adopts high-pressure homogeneously to carry out pollen process; Chinese patent CN201010171690.9 discloses a kind of tea plant pollen wall breaking technology, first adopts enzymolysis to carry out pollen process by method that is freezing, that pulverize again; Chinese patent CN93104588.6 discloses a kind of pollen broken wall and nutriment extractive technique, adopts method that is freezing and Mechanical Crushing to carry out pollen process; Chinese patent CN201010287933.5 discloses a kind of biological wall breaking technology of pollen pini, adopts complex enzyme formulation to carry out pollen enzymolysis processing; Chinese patent CN98104558.8 discloses a kind of technology for broken wall of melissa pollen, adopts the method for the temperature difference and fermentable to carry out pollen process.The people such as He Yutang adopt temperature differential method, supercritical ultrasonics technology, enzyme process to carry out broken wall treatment to zasiokaurin, and sporoderm-broken rate reaches about 50%; The people such as Ren Xiangnan study the research of ultrasonic wave to rape pollen broken wall treatment, result shows: adopt separately ultrasonic wave exposore to come off, and inwall is comparatively complete, affect the stripping of content, in addition, adopt the technique study of enzymolysis to find, papain is to rape pollen enzymolysis 20h, wearing the color index of white powder is 0.78, and protein solubility index is 0.56; The people such as Cao Longkui carry out broken wall treatment by ultramicro grinding to zasiokaurin, and result shows: under pollen water content is less than the prerequisite of 5%, rotating speed reaches 176r/min, pulverize 1.2h, and pollen can reach nearly 100% fragmentation.
There is many problems in the patent of existing pollen broken wall and research, ultrasonic wave process can well retain the active material of pollen, but rate of breaking pollen wall is lower, affects the stripping of active material.Independent enzymatic treatment DeGrain, the combined-enzyme method process processing time is long, processing procedure is loaded down with trivial details, the shortcoming such as expensive of enzyme preparation.Although ultramicro grinding process effect is better, equipment is more expensive, and the requirement for raw material is relatively stricter, and processing method is violent, produces huge heat and has adverse effect significantly for pollen Middle nutrition material and local flavor.So seek that a kind of technique is simple, easy and simple to handle, shell-broken effect good, industrialized processing method can be carried out and be necessary very much.
Summary of the invention
In order to overcome the above-mentioned shortcoming of prior art with not enough, a kind of supersonic synergic impulse electric field is the object of the present invention is to provide to prepare the method for pollen nutrient liquid, the rate of breaking pollen wall obtained in pollen nutrient liquid is higher, technique is simple, not through high-temperature process in leaching process, reduce the destruction of the nutriment in pollen.
Object of the present invention is achieved through the following technical solutions:
Supersonic synergic impulse electric field prepares a method for pollen nutrient liquid, comprises the following steps:
(1) deimpurity pollen will be gone to add in Adlerika, after mixing, obtain pollen solution;
(2) ultrasonic pre-treatment is carried out to pollen solution;
(3) high-pressure pulse electric process is carried out to the pollen solution after step (2) process, obtain pollen nutrient liquid.
Described supersonic synergic impulse electric field prepares the method for pollen nutrient liquid, further comprising the steps of:
Carry out centrifugal to the pollen nutrient liquid that step (3) obtains, obtain supernatant; De-salting operation is carried out to supernatant, obtains the pollen nutrient liquid refined.
The preparation process of the pollen solution of step (1) is specially:
Be that 0.5-1mol/L food grade magnesium sulfate solution mixes by pollen and concentration, wherein pollen: the mass ratio of Adlerika is 1:10-20, incorporation time 5-10min, and temperature is 40-60 DEG C, mixing speed 500-1000r/min.
The described ultrasonic pre-treatment of step (2) is specially:
Temperature 40-60 DEG C, supersonic frequency 600W-800W, sonication treatment time 5-10min.
The described high-pressure pulse electric process of step (3), is specially:
Temperature 40-60 DEG C, electric-field intensity 10-20kV/cm, impulse waveform is rectangular wave, pulse width 5-10 μ s, pulse frequency 400-1000Hz, and it is 5000-10000 that pollen solution accepts Electric Pulse Treatment number.
Repeat step (3) 2 ~ 3 times.
Described centrifugal, be specially:
Rotating speed 5000-10000r/min, time 1-5min.
Described de-salting operation, is specially:
Adopt dialysis operation, dialysis membrane aperture 5000-10000Da, the time is 10-30min.
Principle of the present invention is as follows: the electrolyte solution in advance by adding certain ionic strength soaks pollen, can change the stripping that membrane passage is conducive to content.After this, ion not only can be sent in pollen cell by fully to adopt ultrasonic wave process to produce cavitation, polarization potential is formed to entering between intracellular charged ion and extracellular ionic environment under follow-up high-pressure pulse electric (PEF) process effect, enhance the shell-broken effect of impulse electric field, and for exposore, there is good destruction.PEF process applies the high voltage of short pulse to pollen particles; polar substances moves to electrode direction at a high speed under the effect of electric field; utilize electroporation of cell membrane principle; cause histiocytic irreversible breaking; thus promote the stripping of bioactivator; and PEF processing procedure can not cause the rising of material temperature, effectively can protect the physiologically active of extract, to the extraction of intracellular matter, there is good effect.Adopted the process of high-pressure pulse electric by the pollen solution after ultrasonic pre-treatment again, not only can reach sufficient destruction to pollen cell wall, and accelerate the stripping of pollen content.
Compared with prior art, the present invention has the following advantages and beneficial effect:
(1) rate of breaking pollen wall in pollen nutrient liquid of the present invention is higher, reaches more than 98%; Pollens nutrition material dissolution rate is high, and wherein flavonoid content can reach more than 24mg/g, and Flavonoid substances is Quercetin, Kaempferol, Isorhamnetin and naringenin.
(2) present invention process is simple, not through high-temperature process in leaching process, reduces the destruction of the nutriment in pollen.
(3) the refining pollen nutrient liquid that the present invention obtains is flaxen clear solution, has unique pollen fragrance, nutritiously can be applied to food, the field such as health products and cosmetics.
Accompanying drawing explanation
Fig. 1 is the process chart that supersonic synergic impulse electric field of the present invention prepares the method for pollen nutrient liquid.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
Take the Qinghai-Tibet rape pollen 500g of impurity elimination.Be that 1mol/L magnesium sulfate (food-grade) solution mixes by pollen and concentration, obtain pollen solution, wherein, pollen: the mass ratio 1:20 of Adlerika, incorporation time 10min, temperature is 60 DEG C, mixing speed 1000r/min.Carry out ultrasonic wave (SB-25-12D supersonic wave cleaning machine, Ningbo Xin Zhi biotechnology joint-stock company) pretreatment process to pollen solution, ultrasonic treatment conditions are temperature 60 C, supersonic frequency 800W, sonication treatment time 10min.Pollen solution after ultrasonic pre-treatment is carried out high-pressure pulse electric (south China science and engineering light industry and food are developed) to process, through liquor pump, pollen solution is transported to high-pressure pulse electric, its treatment conditions are: temperature 60 C, electric-field intensity 10kV/cm, impulse waveform is rectangular wave, pulse width 10 μ s, pulse frequency 1000Hz, it is 10000 that sample accepts Electric Pulse Treatment number, and in order to improve sporoderm-broken rate, circulate above-mentioned high-pressure pulse electric process 2 times.Pollen solution after process is carried out centrifugally operated (TGL-15B centrifuge, Town in Shanghai booth tech equipment factory), and centrifugal condition is rotating speed 10000r/min, and time 1min obtains pollen supernatant.Finally carry out dialysis operation (base bio tech ltd, Shanghai one) to pollen supernatant, dialysis technique is dialysis membrane aperture 10000Da, and the time is 60min, obtains refining pollen nutrient liquid.The method sporoderm-broken rate reaches 99%, and wherein flavones content is 24.1mg/g, and sporoderm-broken rate is higher than 85% of enzyme process, and the sporoderm-broken rate of normal ultrasound waves process under the same terms is only about 65%, and the sporoderm-broken rate of the simple PEF process of the same terms is 50-60%.
Embodiment 2
Take the Qinghai-Tibet rape pollen 100g of removal of impurities, be that 0.5mol/L magnesium sulfate (food-grade) solution mixes by pollen and concentration, obtain pollen solution; Wherein, pollen: the mass ratio 1:10 of Adlerika, incorporation time 5min, temperature is 40 DEG C, mixing speed 500r/min.Carry out ultrasonic wave (with embodiment 1) pretreatment process to pollen solution, ultrasonic treatment conditions are temperature 40 DEG C, supersonic frequency 600W, sonication treatment time 5min.Pollen solution after ultrasonic pre-treatment is carried out high-pressure pulse electric (with embodiment 1) process, its treatment conditions are: temperature 40 DEG C, electric-field intensity 10kV/cm, impulse waveform is square wave, pulse width 5 μ s, pulse frequency 400Hz, it is 5000 that sample accepts Electric Pulse Treatment number, and circulate above-mentioned high-pressure pulse electric process 2 times.Pollen solution after process is carried out centrifugally operated (with embodiment 1) and obtains pollen supernatant, centrifugal condition is rotating speed 5000r/min, time 5min.Finally carry out dialysis operation (with embodiment 1) to pollen supernatant, dialysis technique is dialysis membrane aperture 5000Da, and the time is 30min, obtains refining pollen nutrient liquid.The average sporoderm-broken rate of the method reaches 98%, and wherein flavones content is 24.5mg/g, and sporoderm-broken rate is higher than 85% of enzyme process.And the sporoderm-broken rate of normal ultrasound waves process under the same terms is only about 60%, the sporoderm-broken rate of the simple PEF process of the same terms is 50-55%.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not limited by the examples; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. supersonic synergic impulse electric field prepares a method for pollen nutrient liquid, it is characterized in that, comprises the following steps:
(1) deimpurity pollen will be gone to add in Adlerika, after mixing, obtain pollen solution;
(2) ultrasonic pre-treatment is carried out to pollen solution;
(3) high-pressure pulse electric process is carried out to the pollen solution after step (2) process, obtain pollen nutrient liquid.
2. supersonic synergic impulse electric field according to claim 1 prepares the method for pollen nutrient liquid, it is characterized in that, further comprising the steps of:
Carry out centrifugal to the pollen nutrient liquid that step (3) obtains, obtain supernatant; De-salting operation is carried out to supernatant, obtains the pollen nutrient liquid refined.
3. supersonic synergic impulse electric field according to claim 1 prepares the method for pollen nutrient liquid, it is characterized in that, the preparation process of the pollen solution of step (1) is specially:
Be that 0.5-1mol/L food grade magnesium sulfate solution mixes by pollen and concentration, wherein pollen: the mass ratio of Adlerika is 1:10-20, incorporation time 5-10min, and temperature is 40-60 DEG C, mixing speed 500-1000r/min.
4. supersonic synergic impulse electric field according to claim 1 prepares the method for pollen nutrient liquid, it is characterized in that, the described ultrasonic pre-treatment of step (2) is specially:
Temperature 40-60 DEG C, supersonic frequency 600W-800W, sonication treatment time 5-10min.
5. supersonic synergic impulse electric field according to claim 1 prepares the method for pollen nutrient liquid, it is characterized in that, the described high-pressure pulse electric process of step (3), is specially:
Temperature 40-60 DEG C, electric-field intensity 10-20kV/cm, impulse waveform is rectangular wave, pulse width 5-10 μ s, pulse frequency 400-1000Hz, and it is 5000-10000 that pollen solution accepts Electric Pulse Treatment number.
6. supersonic synergic impulse electric field according to claim 5 prepares the method for pollen nutrient liquid, it is characterized in that, repeats step (3) 2 ~ 3 times.
7. supersonic synergic impulse electric field according to claim 2 prepares the method for pollen nutrient liquid, it is characterized in that, described centrifugal, is specially:
Rotating speed 5000-10000r/min, time 1-5min.
8. supersonic synergic impulse electric field according to claim 2 prepares the method for pollen nutrient liquid, it is characterized in that, described de-salting operation, is specially:
Adopt dialysis operation, dialysis membrane aperture 5000-10000Da, the time is 10-30min.
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