CN111892664A - Preparation method of saponin rice polysaccharide gum - Google Patents
Preparation method of saponin rice polysaccharide gum Download PDFInfo
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- CN111892664A CN111892664A CN202010759831.2A CN202010759831A CN111892664A CN 111892664 A CN111892664 A CN 111892664A CN 202010759831 A CN202010759831 A CN 202010759831A CN 111892664 A CN111892664 A CN 111892664A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a preparation method of saponin rice polysaccharide gum, belonging to the technical field of food additives. The preparation method of the saponin rice polysaccharide gum comprises the following steps: step 1: freezing and pulverizing semen Gleditsiae at low temperature, and mixing with the water solution to obtain mixed solution; step 2: heating the mixed solution to a preset temperature, introducing ultrasonic waves with a preset frequency during heating, and reacting for a period of time to obtain a reaction solution; and step 3: centrifuging the obtained reaction solution, taking supernatant, adding ethanol solution into the supernatant, taking precipitate, and drying to obtain saponin rice polysaccharide gum. The invention adopts the low-temperature freezing and crushing technology to crush the high-quality Chinese honeylocust fruit rice and put the crushed Chinese honeylocust fruit rice into the aqueous solution, and simultaneously, ultrasonic assistance is introduced during heating, so that the solution in the system is stirred at a high speed, the mass transfer effect among phases is greatly accelerated, and the polysaccharide gum of the Chinese honeylocust fruit rice can be obtained as much as possible.
Description
Technical Field
The invention relates to the technical field of food additives, in particular to a preparation method of saponin rice polysaccharide gum.
Background
The Chinese honeylocust fruit is commonly called as saussurea involucrate, Chinese honeylocust kernel and Chinese honeylocust essence and is the fruit of Chinese honeylocust fruit. Belongs to a high-energy, high-carbohydrate, low-protein and low-fat food. And contains vegetable dietary fiber. Has effects in nourishing heart, dredging collaterals, removing liver fire, and improving eyesight. And the saponin rice contains a large amount of amino acids, plant polysaccharides and mineral elements, and has dietary therapy and medical therapy values for human bodies. The plant polysaccharide gum extracted from the interior of the Chinese honeylocust fruit can be used as a food additive to be applied to the life of people, and the additive not only can replace the guar gum imported internationally, but also has the effects of maintaining beauty, keeping young, enhancing immunity and the like.
In order to extract polysaccharide gum from Chinese honeylocust seeds, the traditional method is to soak in water and then centrifugally dry, and the method has the disadvantages of complicated process, long time consumption, low extraction rate and low quality of obtained products.
Disclosure of Invention
The invention aims to provide a preparation method of saponin rice polysaccharide gum, aiming at solving the technical problems in the background technology, and the inventor finds that the extraction time can be shortened and the extraction rate of saponin rice polysaccharide gum can be increased by adding a low-temperature freezing and crushing technology on the basis of the prior art to fully contact a product with an aqueous solution and adopting ultrasonic assistance.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1: freezing and pulverizing semen Gleditsiae at low temperature, and mixing with the water solution to obtain mixed solution;
step 2: heating the mixed solution to a preset temperature, introducing ultrasonic waves with a preset frequency during heating, and reacting for a period of time to obtain a reaction solution;
and step 3: centrifuging the obtained reaction solution, taking supernatant, adding ethanol solution into the supernatant, taking precipitate, and drying to obtain saponin rice polysaccharide gum.
In the step 1, the temperature for low-temperature freeze pulverization is-50 ℃ to-100 ℃.
In the step 2, the preset temperature is 50-80 ℃.
In the step 2, the preset frequency is 23kHz-28 kHz.
In the step 2, the reaction time is 0.8h-1.2 h.
In the step 2, in the process of heating and stabilizing the mixed solution to the preset temperature, the temperature rise rate is controlled to be 3-5 ℃/min.
In the step 3, the mass fraction of the ethanol solution is 70-90%.
Compared with the prior art, the invention has the beneficial effects that:
the invention adopts the low-temperature freezing and crushing technology to crush the high-quality Chinese honeylocust fruit rice and put the crushed Chinese honeylocust fruit rice into the aqueous solution, and simultaneously, ultrasonic assistance is introduced during heating, so that the solution in the system is stirred at a high speed, the mass transfer effect among phases is greatly accelerated, and the polysaccharide gum of the Chinese honeylocust fruit rice can be obtained as much as possible.
Detailed Description
The present invention will be further described with reference to the following examples, which are intended to illustrate only some, but not all, of the embodiments of the present invention. Based on the embodiments of the present invention, other embodiments used by those skilled in the art without any creative effort belong to the protection scope of the present invention.
Example 1:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1, selecting 1 part of Chinese honeylocust fruit rice, freezing and crushing at the low temperature of-50 ℃, and mixing with an aqueous solution to obtain a mixed solution.
And 2, heating the mixed solution to 50 ℃, controlling the heating rate at 3 ℃/min, introducing ultrasound while heating, reacting for 1 hour at the frequency of 23kHz, and obtaining a reaction solution.
And 3, centrifuging the reaction liquid, taking supernatant, adding 80% ethanol solution into the supernatant, taking the precipitate, and drying to obtain the saponin rice polysaccharide gum.
Example 2:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1, selecting 1 part of Chinese honeylocust fruit rice, freezing and crushing at the low temperature of-70 ℃, and mixing with an aqueous solution to obtain a mixed solution.
And 2, heating the mixed solution to 50 ℃, controlling the heating rate at 4 ℃/min, introducing ultrasound while heating, reacting for 0.9 hour at the frequency of 25kHz, and obtaining a reaction solution.
And 3, centrifuging the reaction liquid, taking supernatant, adding 85% ethanol solution into the supernatant, taking precipitate, and drying to obtain the saponin rice polysaccharide gum.
Example 3:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1, selecting 1 part of Chinese honeylocust fruit rice, freezing and crushing at the low temperature of-50 ℃, and mixing with an aqueous solution to obtain a mixed solution.
And 2, heating the mixed solution to 60 ℃, controlling the heating rate at 5 ℃/min, introducing ultrasound while heating, reacting for 1 hour at the frequency of 28kHz, and obtaining a reaction solution.
And 3, centrifuging the reaction liquid, taking supernatant, adding 80% ethanol solution into the supernatant, taking the precipitate, and drying to obtain the saponin rice polysaccharide gum.
Example 4:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1, selecting 1 part of Chinese honeylocust fruit rice, freezing and crushing at the low temperature of-50 ℃, and mixing with an aqueous solution to obtain a mixed solution.
And 2, heating the mixed solution to be stable to 80 ℃, controlling the heating rate to be 5 ℃/min, introducing ultrasonic waves during heating, reacting for 0.9 hour at the frequency of 28kHz, and obtaining reaction liquid.
And 3, centrifuging the reaction liquid, taking supernatant, adding 80% ethanol solution into the supernatant, taking the precipitate, and drying to obtain the saponin rice polysaccharide gum.
Example 5:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1, selecting 1 part of Chinese honeylocust fruit rice, freezing and crushing at a low temperature of-100 ℃, and mixing with an aqueous solution to obtain a mixed solution.
And 2, heating the mixed solution to 50 ℃, controlling the heating rate at 5 ℃/min, introducing ultrasound while heating, reacting for 1 hour at the frequency of 25kHz, and obtaining a reaction solution.
And 3, centrifuging the reaction liquid, taking supernatant, adding 80% ethanol solution into the supernatant, taking the precipitate, and drying to obtain the saponin rice polysaccharide gum.
Example 6:
a method for preparing saponin rice polysaccharide gum comprises the following steps:
step 1, selecting 1 part of Chinese honeylocust fruit rice, freezing and crushing at the low temperature of-50 ℃, and mixing with an aqueous solution to obtain a mixed solution.
And 2, heating the mixed solution to 50 ℃, controlling the heating rate at 4 ℃/min, introducing ultrasound while heating, reacting for 1 hour at the frequency of 23kHz, and obtaining a reaction solution.
And 3, centrifuging the reaction liquid, taking supernatant, adding 70% ethanol solution into the supernatant, taking the precipitate, and drying to obtain the saponin rice polysaccharide gum.
Detecting the extraction rate, comprising the following steps:
a. the sample was crushed and dissolved in a 500mL volumetric flask. Taking 2mL of the solution in another volumetric flask, and diluting to 500 mL;
b. 1mL of the diluted solution was taken in a test tube, 4mL of anthrone reagent was added in parallel to three thirds, and the extract was replaced with equal amount of distilled water in a blank tube.
c. The polysaccharide content was determined using a spectrophotometer.
Wherein: c-sugar content (μ g) as found on the standard curve,
vtotal-total volume of extract (mL),
v assay-volume taken at assay (mL),
d is the dilution multiple of the solution,
w-weight of sample (g),
106-weight unit of sample converted from g to μ g times
The feedback results of the above embodiments are as follows:
example 1: the extraction rate is 34 percent
Example 2: the extraction rate is 37%
Example 3: the extraction rate is 30 percent
Example 4: the extraction rate is 27 percent
Example 5: the extraction rate is 32 percent
Example 6: the extraction rate is 29 percent
The above examples are provided to show the effect of increasing the extraction rate of polysaccharide gum from Chinese honey locust seeds, and the above embodiments are merely to describe the preferred embodiments of the present invention, and not to limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims (7)
1. The preparation method of the saponin rice polysaccharide gum is characterized by comprising the following steps:
step 1: freezing and pulverizing semen Gleditsiae at low temperature, and mixing with the water solution to obtain mixed solution;
step 2: heating the mixed solution to a preset temperature, introducing ultrasonic waves with a preset frequency during heating, and reacting for a period of time to obtain a reaction solution;
and step 3: centrifuging the obtained reaction solution, taking supernatant, adding ethanol solution into the supernatant, taking precipitate, and drying to obtain saponin rice polysaccharide gum.
2. The method for preparing saponin rice polysaccharide gum according to claim 1, wherein the temperature for low-temperature freeze pulverization in the step 1 is-50 ℃ to-100 ℃.
3. The method of claim 1, wherein the predetermined temperature in step 2 is 50 ℃ to 80 ℃.
4. The method of claim 1, wherein in step 2, the predetermined frequency is 23kHz to 28 kHz.
5. The method of claim 1, wherein in step 2, the reaction time is 0.8h to 1.2 h.
6. The method for preparing saponin rice polysaccharide gum according to claim 1, wherein in the step 2, the temperature rising rate is controlled to be 3-5 ℃/min during the process of heating and stabilizing the mixed solution to the preset temperature.
7. The method for preparing saponin rice polysaccharide gum according to claim 1, wherein in the step 3, the mass fraction of the ethanol solution is 70-85%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113854564A (en) * | 2021-09-29 | 2021-12-31 | 无锡职业技术学院 | Preparation method of endurance running energy glue |
| CN114989318A (en) * | 2022-05-09 | 2022-09-02 | 孙芙蓉 | Moisturizing and whitening polysaccharide and application thereof in preparing moisturizing and whitening cosmetics |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060935A (en) * | 2010-12-10 | 2011-05-18 | 上海工程技术大学 | Selenylation honeylocust kernel polysaccharide gum and preparation method thereof |
| CN102336841A (en) * | 2011-10-18 | 2012-02-01 | 中国计量学院 | Method for extracting tea polysaccharide |
-
2020
- 2020-07-31 CN CN202010759831.2A patent/CN111892664A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060935A (en) * | 2010-12-10 | 2011-05-18 | 上海工程技术大学 | Selenylation honeylocust kernel polysaccharide gum and preparation method thereof |
| CN102336841A (en) * | 2011-10-18 | 2012-02-01 | 中国计量学院 | Method for extracting tea polysaccharide |
Non-Patent Citations (3)
| Title |
|---|
| 张志锋等: "《彝药化学（第1版）》", 31 August 2017, 民族出版社 * |
| 徐锡媛等: "响应面分析法优化白籽仁多糖提取工艺的研究", 《中国食品添加剂》 * |
| 顾维雄主编: "《保健食品（第1版）》", 31 January 2001, 上海人民出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113854564A (en) * | 2021-09-29 | 2021-12-31 | 无锡职业技术学院 | Preparation method of endurance running energy glue |
| CN114989318A (en) * | 2022-05-09 | 2022-09-02 | 孙芙蓉 | Moisturizing and whitening polysaccharide and application thereof in preparing moisturizing and whitening cosmetics |
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