CN114989318A

CN114989318A - Moisturizing and whitening polysaccharide and application thereof in preparing moisturizing and whitening cosmetics

Info

Publication number: CN114989318A
Application number: CN202210497741.XA
Authority: CN
Inventors: 孙芙蓉
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-05-09
Filing date: 2022-05-09
Publication date: 2022-09-02

Abstract

The invention discloses a moisturizing and whitening polysaccharide and application thereof in preparing moisturizing and whitening cosmetics, wherein the polysaccharide is prepared by the following steps: picking flower buds of the southwest camellia, drying and crushing at 50 ℃; taking bud powder, heating and extracting at 75 ℃ for degreasing by taking an ethanol water solution with the volume percentage concentration of 95% as a solvent, then adding distilled water into residues, heating and extracting at 75 ℃ for 1.5h each time for 3 times, combining extracting solutions, concentrating the extracting solution, filtering absorbent cotton, adding 4 times of absolute ethanol into filtrate, uniformly mixing, standing, performing suction filtration, washing filter residues with absolute ethanol and acetone in sequence, performing deproteinization by a Sevag method, and performing freeze drying to obtain the bud polysaccharide of the southwestern red camellia. The flower bud polysaccharide of the camellia japonica thunb provided by the invention has excellent whitening and moisturizing activities, and has a prospect of developing whitening and moisturizing active factors in whitening and moisturizing cosmetics.

Description

Moisturizing and whitening polysaccharide and application thereof in preparing moisturizing and whitening cosmetics

Technical Field

The invention belongs to the field of macromolecules, relates to polysaccharide macromolecular components, and particularly relates to a moisturizing and whitening polysaccharide and application thereof in preparation of moisturizing and whitening cosmetics.

Background

With the development of social economy and the continuous improvement of living standard of people, the beauty concept of people gradually turns to healthy beauty. Therefore, cosmetics developed from natural nontoxic, therapeutic and nutritional substances are favored by consumers. Oriental women have been advocated for white and fair skin all the time, and cosmetics with whitening (moisturizing) efficacy have become an important research field of research institutions and cosmetic companies, so that whitening (moisturizing) cosmetics develop rapidly.

Southwest red Camellia (Camellia Pitardii coh. stuart) is a Camellia (Camellia) plant of the family Theaceae, and is distributed from southwest to south of the four Sichuan provinces, Guizhou, northern Guangxi, western Hunan, western Hubei and Yunnan in China, so that the resource is very rich. Research shows that various different parts of the southwest red camellia have various biological activities. However, the small molecular compounds contained in camellia sinensis in southwest are mainly developed, and the research on the large molecular compounds is very little.

Disclosure of Invention

The invention aims to provide a moisturizing and whitening polysaccharide and application thereof in preparing moisturizing and whitening cosmetics.

The purpose of the invention is realized by the following scheme:

a moisturizing and whitening polysaccharide is prepared by the following steps: picking flower buds of the southwest camellia, drying and crushing at 50 ℃; taking bud powder, heating and extracting at 75 ℃ for degreasing by taking an ethanol water solution with the volume percentage concentration of 95% as a solvent, then adding distilled water into residues, heating and extracting at 75 ℃ for 1.5h each time for 3 times, combining extracting solutions, concentrating the extracting solution, filtering absorbent cotton, adding 4 times of absolute ethanol into filtrate, uniformly mixing, standing, performing suction filtration, washing filter residues with absolute ethanol and acetone in sequence, performing deproteinization by a Sevag method, and performing freeze drying to obtain the bud polysaccharide of the southwestern red camellia.

Preferably, the drying condition in the above step is 50 ℃ for 72 h.

Preferably, the material-liquid ratio in the steps is 1kg to 5L.

Preferably, the defatting extraction in the above step is performed for 2 times, each time for 1.5 h.

Preferably, absolute ethyl alcohol is added in the steps, and the mixture is kept stand for 12 hours after being uniformly mixed.

The application of any one of the moisturizing and whitening polysaccharides in preparing whitening and moisturizing cosmetics.

Preferably, the whitening and moisturizing cosmetic is a whitening and moisturizing mask.

Preferably, the formula of the whitening and moisturizing mask is as follows:

has the beneficial effects that:

the flower bud polysaccharide of the camellia japonica thunb provided by the invention has excellent whitening and moisturizing activities, and has a prospect of developing whitening and moisturizing active factors in whitening and moisturizing cosmetics.

Drawings

FIG. 1 is a scanning electron microscope image of flower bud polysaccharide;

FIG. 2 is a tyrosinase inhibition curve.

Detailed Description

Example 1:

picking flower bud of southwestern red Camellia (Camellia Pitardii Coh. Stuart), baking at 50 deg.C for 72 hr, pulverizing, and sieving with 40 mesh sieve. Extracting 5kg of powder with 95% ethanol (volume percentage, the same below) at 75 deg.C for 2 times at a ratio of 1kg to 5L for 1.5 hr; adding distilled water into the residue, heating and extracting at 75 deg.C for 1.5 hr at a ratio of material to liquid of 1kg:5L for 3 times, mixing extractive solutions, concentrating to 5L, filtering absorbent cotton to remove precipitate generated during concentration, adding 20L anhydrous ethanol into the filtrate, mixing, standing for 12 hr, vacuum filtering, washing the residue with anhydrous ethanol and acetone in sequence, deproteinizing by Sevag method, and freeze drying to obtain bud polysaccharide of Camellia sinensis L.

The content of polysaccharide in the bud polysaccharide is measured by a phenol-sulfuric acid method, and the three measurement results are 95.3%, 96.5% and 95.7% respectively.

The SEM appearance of the bud polysaccharide is observed by a scanning electron microscope, and the result is shown in figure 1, wherein A is the observation result magnified by 400 times, and B is the observation result magnified by 10000 times, so that the appearance is good.

Example 2:

the tyrosinase inhibitory activity of the flower bud polysaccharide of the southwestern red camellia is determined by referring to a method disclosed by Wudeoson Master of Yunnan province pharmaceutical research institute (Chaihefeng, etc.: evaluation of antioxidant and whitening effects of the white plum blossom extract; Vol. 52, No. 2 of daily chemical industry).

The specific method comprises the following steps:

weighing a proper amount of bud polysaccharide (prepared by the method of example 1), preparing polysaccharide sample solution with mass concentration of 12.5, 25, 50, 100 and 200 mu g/mL by PBS, taking kojic acid as a positive control (kojic acid is purchased from a source leaf, the purity is more than or equal to 98 percent, the concentration gradient is the same as the polysaccharide sample solution), and adding 100 mu L of sample solution or kojic acid solution into a 96-well plate. Adding 50 μ L tyrosinase solution (tyrosinase is from original leaf, product number S10171-25KU, enzyme activity in tyrosinase solution is 5U/mL) into each well, reacting at room temperature in dark for 10min, adding 50 μ L L-tyrosinase solution (Perfemiker, purity is greater than or equal to 98%, L-tyrosine concentration is 1.0 mg/mL). The blank control replaced the sample with PBS, the sample control replaced tyrosinase with PBS, and the rest of the procedure was the same. The reaction solution was mixed well and reacted for 30min at 37 ℃ in the dark. The absorbance OD was measured at 492nm and the tyrosinase inhibition was calculated according to the formula: tyrosinase inhibition (%) [1- (sample OD value-sample control OD value)/blank control OD value ] × 100%. Data were analyzed using SPSS 19.0 statistical software and expressed as mean ± standard deviation.

The results are shown in table 1 and fig. 2, and the southwestern camellia bud polysaccharide and the positive control have similar dose-dependent inhibition effects on tyrosinase, and the difference is that the southwestern camellia bud polysaccharide has slightly weaker inhibition effect on tyrosinase than the positive control at low concentration, but has slightly stronger inhibition effect on tyrosinase than the positive control at high concentration.

TABLE 1 tyrosinase inhibition

Example 3:

bud polysaccharide (prepared according to the method of example 1) 1.0g is weighed into a weighing bottle, sodium alginate (purchased from alatin, product number S100126-100g) is used as a positive control, and the bud polysaccharide with the same weight is weighed, 5 parts each. Then adding 0.4g of distilled water into each weighing bottle, adding the distilled water onto the surface of the sample as much as possible when the distilled water is added, placing the weighing bottles into a drier after the sample fully absorbs moisture, storing the samples at normal temperature, weighing the samples for 6 hours, 12 hours, 18 hours and 24 hours respectively, weighing each weighing bottle for 3 times, and calculating the moisture retention rate according to a formula: moisture retention (%) — moisture weight contained/initial moisture weight × 100% at different time points.

Data were analyzed using SPSS 19.0 statistical software and expressed as mean ± standard deviation. The results are shown in table 2, and the moisturizing performance of the flower bud polysaccharide of the camellia japonica in southwest is superior to that of the positive control.

TABLE 2 moisture retention

Example 4:

a whitening and moisturizing mask comprises the following formula:

Claims

1. the moisturizing and whitening polysaccharide is characterized by being prepared by the following steps: picking flower buds of the southwest camellia, drying and crushing at 50 ℃; taking bud powder, heating and extracting at 75 ℃ for degreasing by taking an ethanol water solution with the volume percentage concentration of 95% as a solvent, then adding distilled water into residues, heating and extracting at 75 ℃ for 1.5h each time for 3 times, combining extracting solutions, concentrating the extracting solution, filtering absorbent cotton, adding 4 times of absolute ethanol into filtrate, uniformly mixing, standing, performing suction filtration, washing filter residues with absolute ethanol and acetone in sequence, performing deproteinization by a Sevag method, and performing freeze drying to obtain the bud polysaccharide of the southwestern red camellia.

2. The moisturizing and whitening polysaccharide of claim 1, wherein: the drying condition is 50 ℃ for 72 h.

3. The moisturizing and whitening polysaccharide of claim 1, wherein: the feed-liquid ratio is 1kg to 5L.

4. The moisturizing and whitening polysaccharide of claim 1, wherein: defatting and extracting for 2 times, each for 1.5 hr.

5. The moisturizing and whitening polysaccharide of claim 1, wherein: adding absolute ethyl alcohol, mixing uniformly, and standing for 12 h.

6. Use of the moisturizing and whitening polysaccharides according to any one of claims 1 to 5 for preparing whitening and moisturizing cosmetics.

7. The use according to claim 6, wherein the whitening and moisturizing cosmetic is a whitening and moisturizing mask.

8. The use of claim 7, wherein the formula of the whitening and moisturizing mask is as follows: