KR20100073275A - Cosmetics comprising cistanche tubulosa extract and method of making the same - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Cistanche tubulosa extract is provided to ensure antioxidation and skin cell proliferation and to promote collagen synthesis. CONSTITUTION: A cosmetic composition contains 10,000ug/ml-100ug/ml of Cistanche tubulosa extract. The Cistanche tubulosa extract is obtained by using water, methanole, ethanol, or mixture thereof. A method for manufacturing the Cistanche tubulosa extract comprises: a step of dipping Cistanche tubulosa in 99% or more methanol solution; a step of extracting 1-10 days after immersing; and a step of evaporating methanol solution and filtering.

Description

Cosmetic composition comprising mandarin extract and method for producing the same {cosmetics comprising Cistanche tubulosa extract and method of making the same}

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition having a natural plant extract.

The present invention relates to a mandarin ( cistanche tubulosa ) extract having an antioxidant effect, promoting the proliferation of skin cells, inhibiting skin cell damage caused by peroxides, and promoting the production of collagen, and a cosmetic composition containing the same. More specifically, the present invention is an antioxidant effect extracted from the mandarin using an organic solvent, and promotes proliferation for skin cells and cell protection against peroxides, mandarine extract having a collagen production promoting effect and skin protection and anti-aging cosmetics using the same It relates to a composition.

Cistanche tubulosa, also called 'mandarin', is a plant belonging to the Hamauts family in the Xinjiang Uygur Autonomous Region Takuramakan Desert or Pakistan, and lives in the roots of Tamaricus. Its main mountain is Hotan (和田), an oasis on the Silk Road in the southern part of the Takuramakan Desert. Hotan is a Uighur word meaning 'you can't come back alive twice when you enter.' Reproduction of tubulossa called this 'desert ginseng' and has been treasured.

Sistanze Tubulossa is known to be effective in nourishing tonic, preventing and improving erectile or menopausal disorders, activating brain function and preventing dementia, and is known as Acteoside and Echinacoside, the main active ingredients. Health and Physiological Activity of Native Plant Mandarins)

Cistanche tubulosa , on the other hand, Haihui Xie et al "Monoterpeneconstituents from Cistanche tubulosa-Chemical structures of kankanosides AE and kankanol" (Chem. Pharm. Bull. 54 (5) 669-675, 2006 ), Chen M. et al., "The determination of echinacoside and acteoside in herbs of Cistanche tubulosa" (Zhongguo Zhong Yao ZA Zhi. 30 (11). 839-841, 2005), Song ZH. "Studies on chemical constituents of Cistanche tubulosa (Schenk) R. Wight" (Zhongguo Zhong Yao ZA Zhi. 25 (12). 728-730, 2000), etc. Biological activities such as "Phenylethanoid oligoglycosides and acylated oligosugars with vasorelaxant activity from Cistanche tubulosa" (Bioorg Med Chem. 14 (22). 7468-75, 2006) have been studied.

In addition, "a pharmaceutical preparation containing a phenylethanoid glycoside extracted from a herbaceous plant, cystane tuburosa (Schenk) wig, a preparation method thereof, and use thereof" (Source: Sinpa Pharmaceutical Co. The patent application related to Sistanche tube rosa (Limited) has been filed, but there are no studies related to beauty, skin and skin diseases.

The inventors of the present invention, in connection with the present invention, in order to consider in various aspects the effect of Sistane tuberosa raw material (exactly fleshy stem powder of Sistane tuberosa Schenk Wig) in relation to skin and skin beauty First, the sistane tuberosa was extracted under various conditions, and the useful effects on the skin of each extract were confirmed by DPPH method (antioxidation method using 1.1-diphenyl-2-picrylhydrazyl solution) and SOD (superoxide dismutase) measurement method.

An object of the present invention is to provide a cosmetic composition having a skin protection and anti-aging effect and a method for producing the same, based on such confirmation results.

It is an object of the present invention to provide a cosmetic composition comprising an extract having an antioxidant effect, a cell growth promoting effect, a skin cell protection effect from peroxides, and a collagen production promoting effect, extracted from cistane tuberosa (mandarine) and a method for producing the same. It is done.

The cosmetic composition of the present invention for achieving the above object, the sistanche tubulososa extract containing 100 ㎍ / ㎖ to 10000 ㎍ / ㎖ concentration, the extract is an extractant, each of water, methanol, ethanol and two of them Characterized in that the extract obtained by using one of the set consisting of the above mixture.

When water is used as the extraction solvent, it can be extracted by heating to 100 degrees to 110 degrees Celsius in water at least three times the weight of Sistane Chetuburosa, the raw material for 0.5 to 10 hours.

In the case of ethanol or methanol, an aqueous solution containing 10% or less of water may be used, and unlike water, extraction may be performed at room temperature for a sufficient time, for example, 1 to 10 days, unlike water. Can be.

In the cosmetic composition of the present invention, the extraction solvent is preferably 99% or more aqueous solution of methanol, and preferably 10000 µg / ml to 100 µg / ml based on the weight of Sistane tubulosae. However, even if it contains more than 1000 ㎍ / ㎖ within this range it is effective to include less than 1000 ㎍ / ㎖ because the effect can be reduced compared to the content saturation effect can be reduced, the effect of the invention is sufficiently obtained below 100 ㎍ / ㎖ Can't.

Sistanche tubulo Rosa extraction method for achieving the above object,

Crushing sistane tubulosa to prepare a raw material and immersed in a 99% or more methanol aqueous solution corresponding to three times the weight of the raw material, the extraction of the result of the immersion step at room temperature for 3 days Extraction step of repeating the process three times, (As a result of extracting three days with aqueous methanol solution of three cultures, and again sifted sistane tubulosa raw material to three days immersed in a separate three cultures aqueous methanol solution Repeated three times) may be provided with a filter drying step of removing the extracted raw material through filtration and evaporated aqueous methanol solution to obtain an extract of the dried solid state.

According to the present invention, the test results using human skin cells, it can be seen that it may have an effect on the antioxidant effect, promoting the proliferation and protection of the skin cells, promoting collagen production.

According to the method of the present invention, an extract having an effect related to antioxidant and skin anti-aging can be effectively extracted.

Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples, and Experimental Examples.

First, obtain the respective extracts through the examples of the extraction method of Sistane tubulossa, and find out the effect of these extracts through experimental examples for specific effects while varying the concentration of these extracts, and also, the appropriate amount having the effect Let's find out the range.

Experiments to investigate the effects of DPPH antioxidant activity on the extract 1, experiment to examine the antioxidant activity of the extract using the SOD kits 2, experiments to investigate the cytotoxic and proliferative effect on human skin dermal fibroblast 3, experiment 4 to investigate the protective effect of the solvent-specific extracts on hydrogen peroxide.

In the extraction method, when the extraction solvent is used in an amount of less than 3 times the mass of the sample, the extract cannot be extracted sufficiently, and when the extraction amount is too much, more than 20 times, it may be time and costly to remove the extraction solvent and obtain a dry extract. do. In the case of water, it is necessary to carry out the extraction in a heated state in order to sufficiently include the kinds of extracting substances, and to increase the extraction efficiency. If the temperature is too low, it is difficult to take full advantage of the warming, and the extraction time is too high. Decomposition of useful materials may occur, and may be heated to about 100 to 110 degrees Celsius. If the time is less than 0.5 hours, sufficient extraction cannot be achieved, and if the time is excessively more than 10 hours, the extraction efficiency is lowered, resulting in an inefficient process. When the extraction solvent contains methanol or ethanol, it can be extracted at room temperature, and the powdered sample is immersed in a sufficient extraction solvent for a sufficient time, for example, about 3 days, so that the extraction is sufficient, and the extraction is not sufficient. If not, it is possible to increase the extraction amount through an iterative process. When the extraction is progressed, the method of concentrating the extract first with a rotary vacuum evaporator and then freeze-drying to remove the extraction solvent may be performed. Is common.

(Example Method 1)

As an example in the case of using the Sistane tuberusa extract of the present invention, when water is used as the extraction solvent, 0.1 kg of Sistane tuberosa sample is prepared in order to obtain the water extract of Sistane tuberosa. Sistane tuber Brosa samples are placed in purified water five times the weight and extracted at 105 degrees for 5 hours. At the end of the extraction process, the filter paper was filtered with Whatman paper No. 1, and then concentrated in a rotary vacuum evaporator to remove water, followed by freeze drying. This obtained 14.97 g (dry weight) of Sistane tuberosa water extract.

(Example method 2)

Three times extraction process (95% ethanol) was added to 0.1 kg of Sistane tubulosa sample, followed by three times of shaking extraction at room temperature for 72 hours, followed by filtration of the extract with filter paper and a rotary vacuum evaporator. evaporator) and freeze-drying to obtain 3.24 g (dry weight) of cistane tubulo ethanol extract.

(Example Method 3)

Three times extraction process (99.5% methanol) was added to 0.1 kg of Sistane tubulosa sample, followed by three times of shaking extraction at room temperature for 72 hours, followed by filtration of the extract with filter paper and a rotary vacuum evaporator. Evaporator) and freeze-dried to give 20.53 g (dry weight) of the sistane tuberosa methanol extract.

Experimental Example 1

The cytotoxicity and growth promoting effects of the respective extract samples obtained by the methods of Examples 1, 2 and 3 were measured.

1-1. Experiment method

Cytotoxicity measurements on CCD-986sk human skin fibroblasts were performed by MTT assay, a well-known staining method for measuring viable cell numbers of small cells. MTT assay is a blue formazan product in which live cells reduce MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl-L-tetrazolium bromide, Sigma) by dehydrogenase present in the mitochondria. It is a method of determining the relative growth of living cells by converting to (blue formazan product) and eluting the formed blue product with a solvent to measure its absorbance. More specifically, CCD-986sk (Human dermal skin fibroblast) incubated in 10% FBS-IMDM (Iscove's Modified Dulbecco's Medium, Gibco), and then taken to prepare a cell solution in which the cell concentration was adjusted to 5 × 10 ⁴ cells / ml. Thereafter, 200 μl was dispensed into 96 well-plates, which were incubated for 24 hours in a 37 ° C., 5% CO ₂ incubator. After 24 hours of incubation, the medium was removed, and 200 μl of the extract according to the concentration of Table 1, prepared with 10% FBS-IMDM, and 180 μl of FBS-free IMDM were added thereto, and reacted under the same conditions for 48 hours. At this time, the experiment using only FBS-free IMDM was used as a control of the sample. 3 hours before the completion of the reaction, 20 μl of 5 mg / ml MTT solution (in 1 × PBS (−)) was added, followed by further reaction for 48 hours. After removing the MTT reaction solution, 150 μl of DMSO (dimethyl sulfoxide) was added thereto, followed by stirring for 5 minutes (room temperature), and the absorbance was measured at 570 nm using a microplate reader.

1-2. Experiment result

The results are shown in Table 1 below and graphically shown in FIG. 1.

sample
(Μg / ml) Control 1000 500 250 100 50 25 10 5 One Example 1 100.0% 145.6% 97.9% 104.7% 106.1% 95.4% 106.8% 101.3% 105.6% 103.5% Example 2 100.0% 138.9% 124.6% 107.3% 102.6% 98.3% 97.6% 98.0% 98.2% 99.6% Example 3 100.0% 150.8% 144.2% 112.0% 101.9% 98.3% 98.7% 100.0% 98.2% 98.2%

As shown in Table 1 and FIG. 1, in the case of Sistane tubulo, Examples 1, 2, and 3 did not show cytotoxicity at the maximum concentration of 1,000 µg / ml or less, but in Example 1, 1,000 µg / In the case of ml, Examples 2 and 3 showed a result of promoting concentration-dependent cell proliferation from 250 µg / ml to 1,000 µg / ml. In particular, the methanol extract of Example 3 exhibited the highest cell proliferation promoting effect and showed about 50% higher activity than the control.

Therefore, it was found that the sistanche tuburosa extract has no cytotoxicity and has a proliferation promoting effect on human dermal dermal fibroblasts.

Based on the results, experiments were conducted to examine the anti-oxidation, cell protective effect, and anti-wrinkle effect within the concentration which is not cytotoxic and promotes cell proliferation.

Experimental Example 2

The antioxidant effects of Examples 1, 2, and 3 were measured.

2-1. Free radical scavenging capacity measurement

2-1-1. Experiment method

The electron donating ability (the ability to supply electrons) for the sample was measured as follows. 100 μl of 5 × 10 ⁻⁴ M of DPPH (1-1-diphenyl-2-picryl-hydrazyl) was added to 100 μl of the concentration of each extract, stirred for 1 minute, and then reacted in a 25 ° C. incubator for 30 minutes. The absorbance was measured at 540 nm using a microplate counter.

El-ascorbic acid (1,000µg / ml) was used as an experimental control, and the electron donating ability was expressed as the absorbance reduction rate of the sample solution added and the no added solution.

2-1-2. Experiment result

The results are shown in Table 2 below and graphically shown in FIG. 2.

sample
(Μg / ml) 1000 900 800 700 600 500 400 300 200 100 Example 1 86.3% 84.9% 86.1% 85.9% 84.7% 83.1% 74.7% 56.6% 34.8% 7.6% Example 2 89.3% 85.4% 88.1% 87.2% 84.6% 75.9% 60.9% 43.5% 26.3% 9.1% Example 3 93.8% 93.6% 93.5% 93.2% 92.6% 91.3% 83.3% 78.0% 64.8% 40.0%

As shown in Table 2 and FIG. 2, Examples 1, 2, and 3 all exhibited high free radical scavenging ability. In particular, the methanol extract of Example 3 exhibited an IC ₅₀ of about ₁₅₀ µg / ml, It has been shown to inhibit cell damage and the progress of skin aging. The control group, L-ascorbic acid (1,000 μg / ml), exhibited 95% scavenging activity.

2-2. Superoxide dismutase (SOD) -like activity measurement

2-2-1. Experiment method

SOD-like activity was performed as follows. Into each extract of 20ppm concentration of 100ppm 200μl WST working solution in the superoxide dismutase assay kit (SOD assay kit-WST) was added and stirred, and again the enzyme working solution ( Enzyme working solution) 20 μl were mixed and reacted in a 37 ° C. incubator for 20 minutes, and the absorbance was measured at 450 nm using a microplate counter.

Superoxide dismutase (1,000units) was used as the experimental control, and superoxide dismutase-like activity was expressed as the absorbance reduction rate of the sample solution added and the no added solution.

2-2-2. Experiment result

The results are shown in Table 3 below and graphically shown in FIG. 3.

sample
(Μg / ml) 1000 900 800 700 600 500 400 300 200 100 Example 1 99.5% 78.8% 77.2% 68.4% 63.3% 52.1% 42.9% 43.0% 15.0% 11.7% Example 2 81.3% 77.3% 71.9% 42.7% 59.2% 50.9% 48.9% 32.7% 25.6% 15.0% Example 3 99.8% 99.5% 99.1% 97.9% 97.8% 95.2% 89.4% 77.5% 62.7% 37.2%

As shown in Table 3 and FIG. 3, Examples 1, 2, and 3 are all excellent in the superoxide deskactase-like activity, in particular, in the case of the methanol extract of Example 3, the similar activity of Examples 1 and 2 It showed excellent results maintained at lower concentrations than the case, showing excellent antioxidant and anti-aging effects. In comparison, the superoxide dismutase (1,000 units) showed 99.5% of activity.

Experimental Example 3

The cell protective effect of Examples 1, 2, and 3 was measured.

3-1. Experiment method

In order to measure the effect of inhibiting the damage of CCD-986sk (Human skin dermal fibroblast) by the peroxide was carried out as follows. That is, after incubating the CCD-986sk (Human dermal skin fibroblast) in 10% FBS-IMDM, the cell solution was adjusted to adjust the cell concentration to 2.0 × 10 ⁵ cell / ml, and then 200 μl each on a 96well plate. Aliquots were incubated for 24 hours in a 37 ° C., 5% CO ₂ incubator.

After incubation for 24 hours, after removing the medium, Earle 'solution (0.12M NaCl, 5mM KCl, 0.83mM MgSO ₄ · 7H ₂ O, 5.5mM glucose, 1.8mM CaCl ₂ , 1mM NaH ₂ PO ₄ · 2H ₂ O, 26.2 200 μl of the extract prepared with 1.5 mM H ₂ O ₂ solution in mM NaHCO ₃ in DW) was added again, and the reaction was carried out for 90 minutes under the same conditions. At this time, the experimental sphere prepared using only Earle 'solution was used as a control.

After reacting for 90 minutes, the medium and the solution were removed, 200 μl of 50 μg / ml neutral red (Neutral Red in 10% FBS-IMDM) was added thereto, and the reaction was carried out for 2 hours in a 37 ° C. incubator.

After removing the neutral red reaction solution, 100 μl of 1% CaCl ₂ , 1% formaldehyde (in DW) solution was added thereto, and stirred for 1 minute, and then the solution was removed. Thus, 1% acetic acid and 50% ethanol (in DW) were added. ) Was added again to 100 µl and stirred for 15 minutes, and then the absorbance was measured at 570 nm using a microplate counter.

3-2. Experiment result

The results are shown in Table 4 below and graphically shown in FIG. 4.

sample
(Μg / ml) Control 1000 500 250 100 50 Example 1 100% 108.2% 103.8% 97.2% 96.9% 101.4% Example 2 100% 101.6% 100.6% 101.5% 103.2% 96.9% Example 3 100% 205.1% 194.1% 124.5% 123.5% 108.1%

As shown in Table 4 and Figure 4, in the case of Examples 1 and 2 did not show the effect of inhibiting the cell damage caused by the peroxide, but the methanol extract of Example 3, showed a concentration-dependent cytoprotective effect It showed a cytoprotective effect of about 200% at concentrations of 500 μg / ml and 1,000 μg / ml.

Experimental Example 4

The collagen production promoting effect of Examples 1, 2, and 3 was measured.

4-1. Experiment method

In order to measure the collagen production promoting effect in the CCD-986sk (Human skin dermal fibroblast) was carried out using the SIRCOL collagen assay (Biocolor Ltd) as follows. That is, after incubating the CCD-986sk (Human dermal skin fibroblast) in 10% FBS-IMDM, the cell solution was prepared by adjusting the cell concentration to 1.5 × 10 ⁵ cell / ㎖, and then 2 on a 35 × 10 mm plate Aliquots were dispensed and incubated for 24 hours in a 37 ° C., 5% CO ₂ incubator.

After incubation for 24 hours, the medium was removed, 1 ml of fresh 10% FBS-IMDM was added, and then, 1 ml of each sample prepared for each concentration was added, and cultured again for 24 hours under the same conditions. After incubation for 24 hours, 0.5 ml of the culture solution was taken from each plate, transferred to a microtube, and 1 ml of DYE Reagent (SIRCOL collagen assay, Biocolor Ltd) was added. After stirring for 30 minutes at room temperature, centrifugation (15,000rpm, 5 minutes) to completely remove the supernatant DYE Reagent. 0.1 ml of ALKALI Reagent (SIRCOL collagen assay, Biocolor Ltd) was added thereto, stirred for 5 minutes, and then transferred to a 96well microplate to measure absorbance at 540 nm using a microplate counter. El-ascorbic acid (100 ㎍ / ㎖) was used as an experimental control, collagen biosynthesis promoting effect was expressed as the increase in absorbance compared to the non-added group of the addition of the sample solution.

4-2. Experiment result

The results are shown in Table 5 below and graphically shown in FIG. 5.

1000 100 10 Example 1 96.0% 110.0% 98.2% Example 2 106.0% 97.9% 100.3% Example 3 155.6% 126.5% 106.0%

As shown in Table 5 and Figure 5, Examples 1 and 2 did not participate in collagen biosynthesis in dermal dermal fibroblasts, but methanol extract of Example 3 showed a concentration-dependent collagen biosynthesis promoting effect 100 It showed a collagen biosynthesis promoting effect of about 26% at μg / ml and about 55% at 1,000 μg / ml. In the case of the comparative group, L-ascorbic acid (100 µg / ml), the promoting effect was 100%.

The experimental example shows that most of the characteristics are excellent in the interval of 1000 µg / ml or more, in particular, in the case of Example 3, unlike the other Examples, the concentration of 1000 µg / ml to 100 µg / ml The other extraction method in the section having a high effect even in the portion where the effect is not significant (Experimental Example 3, Experimental Example 4), Experimental Example 2 in the concentration range of 1000㎍ / ㎖ to 100㎍ / ㎖ Compared to the case of using the extract according to the example method, compared to the case of using the other example method, the effect is maintained in Example 3, while the amount of extract decreases proportionally, the effect is maintained in a significant amount. It was found that the effect was excellent in the amount. In particular, in the case of Example 3, it can be determined that the effect is saturated in a section of 1000 µg / ml or more, so that the section having a concentration of 1000 µg / ml to 100 µg / ml has an advantage of the effect than the extract of the other Example method. Could have.

Each cosmetic formulation contains cistane tubulososa extract, which removes free radicals in the skin, protects the skin from cell damage, and promotes the proliferation of skin cells to improve collagen biosynthesis. An embodiment of a cosmetic composition as a formulation having a protective and anti-aging effect is shown and the prescription is as follows. The sistanche tuburosa extract used herein is that of Example 3, which has the most antioxidant and skin cell proliferation promoting effect, cell protective effect, and collagen production promoting effect.

Example Composition 1

Formulation examples of the flexible cosmetic water in the cosmetic containing cistane tubulosa extract according to the present invention is shown in Table 6.

ingredient Content (% by weight) Sistane tuberosa extract (Example 3) 0.01 Polyoxyethylene Cured Castor Oil 0.4 Glycine 3.5 Dipotassium glycylizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

Example Composition 2

Prescription example of nutrient cosmetic water in the cosmetic containing cistane tubulosa extract according to the present invention is as follows.

ingredient Content (% by weight) Sistane tuberosa extract (Example 3) 0.05 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoine 1.0 Stearic acid 1.0 Glyceryl Stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 Purified water Balance

Example Composition 3

Prescription example of nutritional essence in cosmetics containing the sistanche tuburosa extract according to the present invention is as follows.

ingredient Content (% by weight) Sistane tuberosa extract (Example 3) 0.1 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaluronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl Cellulose 0.2 ethanol 3.0 Polyoxyethylene Cured Castor Oil 1.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

Example Composition 4

Prescription example of nutritional cream in the cosmetic containing cistane tubulosa extract according to the present invention is as follows.

ingredient Content (% by weight) Sistane tuberosa extract (Example 3) 0.1 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated Lecithin 1.0 Octyldodecanol 3.0 Trioctanoine 2.0 Stearic acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan Gum 0.2 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

Example Composition 5

Prescription example of the body cream of the cosmetic containing cistane tubulosa extract according to the present invention is as follows.

ingredient Content (% by weight) Sistane tuberosa extract (Example 3) 0.1 1,3-butylene glycol 6.0 glycerin 4.0 Sodium cetyl sulfate 0.1 Panthenol 1.0 Xanthan Gum 1.0 Carbomer 0.1 Biswax 0.3 Cetanol 2.5 Glyceryl Stearate 2.5 Sorbitan stearate 1.5 Mineral oil 5.0 Squalene 2.0 Antioxidant 0.3 Dimethicone 1.0 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

Example Composition 6

Prescription example of the pack of the cosmetic containing cistane tubulosa extract according to the present invention is as follows.

ingredient Content (% by weight) Sistane tuberosa extract (Example 3) 0.1 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyaluronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

As confirmed in the above Examples and Experimental Examples, the sistanche tuburosa extract according to the present invention has an excellent antioxidant effect, promoting the proliferation and cytoprotective effect of the skin cells, promoting collagen biosynthesis, the extract of the present invention Cosmetic compositions prepared by addition also have antioxidant and anti-aging and skin protection effects.

Therefore, according to the present invention, there is an effect of providing a sistanche tubulosa extract and a cosmetic composition comprising the same, which inhibits oxidation to prevent skin cell damage and aging.

Here, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.

Figure 1 is a graph showing the results of experiments on the toxicity and cell proliferation effect while varying the concentration of sistanche tuburosa extract obtained in Examples 1 to 3 of the present invention.

Figure 2 is a graph showing the results of experiments on the antioxidant effect of free radical scavenging ability while varying the concentration of Sistane tubulosa extract obtained in Examples 1 to 3 of the present invention.

Figure 3 is a graph showing the results of testing the antioxidant effect through the measurement of SOD-like activity by varying the concentration of sistane tubulosa extract obtained in Examples 1 to 3 of the present invention.

Figure 4 is a graph showing the results of experiments on the cytoprotective effect while varying the concentration of sistane tubulo Rosa extract obtained in Examples 1 to 3 of the present invention.

Figure 5 is a graph showing the results of experiments to promote the collagenase production by changing the sistanche tuburosa extract obtained in Examples 1 to 3 by concentration.

Claims (5)

It contains Sistane tuberosa extract at a concentration of 10000 µg / ml to 100 µg / ml, The extract is a cosmetic composition, characterized in that the extract obtained by using one of a set consisting of water, methanol, ethanol and a mixture of two or more thereof as an extraction solvent. The method of claim 1, The extraction solvent is 99% or more aqueous solution of methanol, Cosmetic composition, characterized in that it contains the cystane tubulosa extract at a concentration of 1000 μg / ml to 100 μg / ml. An immersion step of preparing a sistane tubuloxa raw material and immersing it in an aqueous 99% or more methanol solution corresponding to 3 to 20 times the weight of the raw material; Extraction step of extracting the result of the immersion step at room temperature for 1 to 10 days, And a filtration drying step of removing the extracted raw material through filtration and evaporating the aqueous methanol solution to obtain an extract in the form of a dried solid. An immersion step of preparing a sistane tubuloxa raw material and immersing it in 90% or more of an ethanol aqueous solution corresponding to 3 to 20 times the weight of the raw material, Extraction step of extracting the result of the immersion step at room temperature for 1 to 10 days, And a filtration drying step of removing the extracted raw material through filtration and evaporating the ethanol aqueous solution to obtain a dried solid extract. An immersion step of preparing a sistane tubuloxa raw material and immersing it in water (H ₂ O) corresponding to 3 to 20 times the weight of the raw material, Performing extraction for 0.5 hours to 10 hours at a temperature of about 100 ° C to 110 ° C, And a filter drying step of removing the extracted raw material through filtration and evaporating an aqueous solution containing the extract to obtain an dried solid extract.

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