KR102247966B1 - Cosmetic Composition Containing Mixture Extracts of Phaseolus Radiatus Seed, Betula Alba Juice and Rumex Crispus Root - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of mung bean seed, Manchurian birch sap, and Sori jaw root. In more detail, it relates to a cosmetic composition having excellent skin moisturizing enhancement, skin irritation relief, and skin wrinkle improvement effect, including a mixed extract of mung bean seed, Manchurian birch sap, and sori chinensis root. The cosmetic composition of the present invention can be widely used for improving various skin problems caused by environmental pollution, stress, etc. and protecting the skin.

Description

Cosmetic Composition Containing Mixture Extracts of Phaseolus Radiatus Seed, Betula Alba Juice and Rumex Crispus Root}

The present invention relates to a cosmetic composition containing a mixed extract of mung bean seed, Manchurian birch sap, and Sori jaw root.

Human skin experiences various skin problems such as dullness, sensitivity, and decrease in moisture retention due to environmental changes, internal and external stresses, etc. In particular, the skin characteristics that are most pronounced due to aging are dry skin and decreased skin elasticity, wrinkles are formed, and the skin becomes dull. In addition, it is easily sensitive to weak external stimuli and it takes a long time to recover.

Therefore, many efforts are being made to solve such skin problems, and as a representative method, natural vegetable ingredients and irritation relievers are used to solve the problem, and these methods are also widely used in the cosmetic industry. Cosmetics are mainly used to keep the skin beautiful and healthy, and to relieve skin troubles and dry skin that occur with age. Recently, cosmetic market trends have made many attempts to develop materials using fermentation products generated by fermentation along with basic properties of natural products by fermenting natural products in addition to using extracts of natural fruits.

Korean Patent Publication No. 2007-011025 discloses a cosmetic composition for improving skin wrinkles containing mung bean extract as an active ingredient.

Korean Patent Registration No. 10-1332214 discloses a cosmetic composition for relieving skin stress comprising a lotus, rice, and mugwort complex fermentation extract.

In the present invention, among various natural plant extracts, a mixture extract of mung bean seed, Manchurian birch sap, and sori chinensis root has a moisturizing effect, skin wrinkles while searching for a material that is superior to the prior art and helps to relieve skin troubles. It was confirmed that it can help to improve the effect and relieve skin irritation caused by skin stress, and accordingly, the present invention was completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition that contains a mixed extract of mung bean seed, Manchurian birch sap, and sori birch root to improve various skin troubles, improve skin wrinkles, and help moisturize the skin.

The present invention is achieved by a cosmetic composition for improving skin wrinkles, comprising a mixed extract of mung bean seed, Manchurian birch sap, and Sori jaw root.

In addition, it provides a cosmetic composition for moisturizing skin comprising a mixed extract of mung bean seed, Manchurian birch sap, and Sorijak root.

In addition, it provides a cosmetic composition for alleviating skin irritation comprising a mixed extract of mung bean seed, Manchurian birch sap, and Sori-jak root.

Preferably, the mixed extract contains 0.0001 to 30% by weight based on the total weight of the cosmetic.

Preferably, the mixed extract may be extracted by an extraction method selected from the group consisting of a solvent extraction method, a fermentation extraction method, an ultra-high pressure treatment extraction method, and an ultra-high pressure extraction method after fermentation.

The mixed extract of mung bean seed, Manchurian birch sap, and Sori chinensis root of the present invention has excellent skin irritation relief effect and excellent anti-wrinkle effect due to the excellent effect of inhibiting MMP-1 production. In addition, the composition of the present invention containing a mixed extract of mung bean seed, Manchurian birch sap, and Sourija root is excellent in skin moisturizing effect. Accordingly, the cosmetic composition of the present invention can be widely used as a cosmetic that protects the skin and improves various skin problems caused by environmental pollution, stress, and the like.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meaning as commonly understood by those of ordinary skill in the relevant fields of the present invention. In addition, although preferred methods or samples are described in the present specification, those similar or equivalent are included in the scope of the present invention. The contents of all publications referred to herein by reference are incorporated into the present invention. The term about refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, or about a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length. It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length, varying by 3, 2 or 1%.

Throughout this specification, unless otherwise required by the context, the terms include and including include the steps or components presented, or groups of steps or components, but any other step or component, or step or component It should be understood to imply that the group of people is not excluded.

The present invention relates to a cosmetic composition containing a mixed extract of mung bean seed, Manchurian birch sap, and sori birch root, and the cosmetic composition according to the present invention has excellent skin moisturization, skin irritation relief, and skin wrinkle improvement effects.

Mung bean ( Phaseolus radiatus , Phaseolus aureus , Mung bean ) is an annual herb that is mainly distributed in Asia, such as Korea, India, and China. Mungdu seeds have been used as an antidote in oriental medicine, and Donguibogam describes that applying mungdu powder to the face can provide a cosmetic effect. In the present invention, nokdu seeds were used.

Manchurian birch ( Betula Alba ) is a plant of the oak tree birch family, which grows in the deep forests of the northern provinces. Birch wood is very hard and dense, making furniture and woodwork. Manchuria birch sap refers to the liquid that penetrates the canopy of the tree trunk and rises to the leaves, and is mainly collected in early spring.

Rumex Crispus ), also known as Crussula, is a perennial plant belonging to the genus Culaceae, native to Europe in the northern hemisphere or distributed in the southern hemisphere. It is known to contain ingredients such as chrysophanol and emordin in Sori-kwi, and has been used to treat gastritis, gastric ulcer, and enteritis due to its excellent anti-inflammatory and sterilizing effects. In the present invention, the roots of a sorrel were used.

The mixed extract of mung bean seed, Manchuria birch sap, and Sori Tzu root used as active ingredients in the composition of the present invention is an extract obtained by mixing the extracts extracted from each of mung bean seed, Manchuria birch sap, and Sori Tzu root, or mung bean seed, Manchuria japonica. It refers to the extract extracted after mixing the tree sap and the sori japonica root.

In addition, the mixed extract of mung bean seed, Manchurian birch sap, and sori birch root may preferably be contained in an amount of 0.0001 to 30% by weight, more preferably 0.001 to 20% by weight, most preferably based on the total weight of the cosmetic composition. It may be included in an amount of 0.01 to 10% by weight. When the content of the extract is less than 0.0001% by weight, the skin improvement effect does not appear, and when it exceeds 30% by weight, the degree of increase in the effect of increasing the content is insignificant, and there is a problem in the safety and stability of the formulation, and is economical. It is also not preferable.

In the present invention, the mixed extract may contain mung bean seeds, Manchuria birch sap, and Sori chinensis root in a weight ratio of 1-3:1-3:1-3, preferably in a weight ratio of 1:1:1. can do.

Grinding extraction among the extraction methods of the mung bean seed extract and the sori birch root extract of the present invention means that the mung bean seed and the sori birch root are pulverized with a fine pulverizer, and the ingredients are filtered through a sieve, and then Manchuria birch sap is mixed. At this time, it is preferable to use the pulverized pulverized product filtered through a sieve of 300 mesh or less.

In addition, solvent extraction in the extraction method of mung bean seed, Manchurian birch sap, and Sori jaw root of the present invention may be prepared using a conventional solvent according to a conventional method known in the art. Typical solvents are, for example, water, anhydrous or aqueous lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1.3-butylene glycol, glycerin, acetone, diethyl ether, ethyl It means extracted with a solvent selected from the group consisting of acetate, butyl acetate, dichloromethane, chloroform, hexane, and mixtures thereof.

In addition, the fermentation extraction of the method for extracting mung bean seeds, Manchuria birch sap and Sori Trang root of the present invention includes an extract obtained by fermenting mung bean seeds, Manchuria birch sap and Sori Trang root. At this time, the fermentation extraction is a fungus selected from the group consisting of mung bean seeds, Manchurian birch sap, and Sori chinensis roots from the group consisting of yeast, lactic acid bacteria, Bacillus bacteria and mixtures thereof, and maintains a pH of 5 to 7 under a temperature condition of 20 to 40°C. It means extracting by fermentation for 1 to 7 days while, preferably, the fungus is Saccharomyces cerevisisae, and most preferably Casaromyces cerevisisae KCTC 7904.

In addition, the ultra-high pressure extraction of the mung bean seed, Manchurian birch sap and Sori Trang root of the present invention includes an extract obtained by ultra-high pressure treatment of the mung bean seed, Manchu birch sap and Sori Trang root. At this time, the ultra-high pressure treatment means treatment for 1 to 24 hours at a temperature of 40 to 90°C at a pressure of 100 to 3000 MPa, and preferably at a temperature of 40 to 60°C and 12 to 24 at a pressure of 500 to 1000 MPa. It is time treatment, and most preferably refers to treatment at a temperature of 50° C. and 24 hours at a pressure of 1000 MPa.

In addition, when the mixed extract of mung bean seed, Manchurian birch sap, and Sori chinensis root of the present invention is extracted by mixing and applying the above-described extraction method, it is possible to obtain an extract that is more effective in improving skin wrinkles, promoting skin moisturization, and improving skin troubles. have. For example, the mixed extract of the present invention is a fungus selected from the group consisting of yeast fungi, lactic acid bacteria, Bacillus bacteria, and other mixtures after mixing the pulverized product of finely pulverized mung bean seeds and sorrel roots with Manchuria birch sap. After fermentation, Mung bean seed obtained by processing the step of ultra-high pressure extraction at a temperature of 40 to 90°C at a pressure of 100 to 3000 ㎫ for 1 to 24 hours, and ultra high pressure treatment extract after fermentation of Mung bean seed, Manchurian birch sap, and Sori jaw root. do.

In addition, the mixed extract of mung bean seed, Manchurian birch sap, and Sori chinensis root of the present invention is filtered to control the concentration of the extract extracted by the above extraction method and increase the concentration of the active ingredient, organic solvent reflux extraction, vacuum concentration, and freezing. You can also go through a process such as drying. It is obvious in the art that the extract obtained by filtering, purifying, and concentrating the extract according to the above-described extraction method is also included in the present invention.

On the other hand, the mixed extract of mung bean seed, Manchurian birch sap, and Sori-jak root of the present invention may be extracted using not only the above-described extraction method, but also other extraction methods.

According to a preferred embodiment of the present invention, after fermentation of mung bean seeds, Manchurian birch sap and Sori jaw root, the ultra-high pressure extract is prepared by the following method. First, after sufficiently washing the mung bean seeds and the roots of sori, pulverized, finely made using 300 mesh, and then mixed with Manchuria birch sap. Mung bean seeds, Manchurian birch sap, and Sori chinensis roots are added to the culture solution of the Saccharomyces genus (Saccharomyces cerevisiae KCTC 7904) so that it becomes 100 g/L. Here, 1 to 4% of glucose (preferably 1 to 2%) as a carbon source and 0.1 to 1% of peptone (preferably 0.2 to 0.5%) as a nitrogen source were added and cultured. The culture was carried out for 1 to 7 days while maintaining the pH at 5-7 under a temperature condition of 20 to 40°C using a 5L fermenter. After cultivation, the culture solution was centrifuged to remove the culture bacteria first. The culture solution of mung bean seed, Manchurian birch sap from which the cultures have been removed, and the sori chinensis root are extracted by ultra-high pressure treatment for 1 to 24 hours at a temperature of 40 to 90°C at a pressure of 100 to 3000 MPa, and preferably 500 to 1000 MPa. The treatment is performed for 12 to 24 hours at a temperature of 40 to 60°C under pressure, and most preferably, the treatment is performed at a temperature of 50°C at a pressure of 1,000 MPa for 24 hours. Ultra-high pressure-treated mung bean seed, Manchurian birch sap, and Sorijak root were extracted with reflux three times for 5 hours by adding 70% (V/V) ethanol aqueous solution, cooled, and filtered with Whatman #2 filter paper. . When filtration is completed, in the present invention, the extract obtained in the extraction step is transferred to a concentration tank, concentrated under reduced pressure, and freeze-dried. The concentration under reduced pressure was concentrated using a rotary vacuum concentrator, and the concentration condition was concentrated under reduced pressure for 2 hours while rotating at 50-70°C and 600-1200 rpm at a pressure of 650mmHg-750mmHg into 700ml of extract in a 1,000ml round flask. The concentrated solution concentrated under reduced pressure is pre-frozen, lowered the temperature to -40°C, maintained at -40°C for 2 to 4 hours, then dropped to -80°C through trap freezing, and the vacuum is maintained at 10 to 30 mTorr. Rise again to -40°C over 120 minutes. After that, it was maintained at -20°C for 240 minutes, 0°C for 40 minutes, 15°C for 340 minutes, 30°C for 130 minutes, and maintained at 1,300°C until completely dried.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the above active ingredients as active ingredients, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, And it may include a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder. .

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

< Example 1> Nokdu seeds, Manchurian Birch Sap And Preparation of Solvent Extract of Sori Jaw Root

500g of Mungbean seeds and 500g of Sorijak root were selected as samples, sufficiently washed, pulverized, and then passed through a 300 mesh sieve to make the pulverized product fine. 500 g of Manchurian birch sap was mixed thereto, and a 70% (V/V) ethanol aqueous solution was added thereto, extracted with reflux three times for 5 hours, cooled, and filtered through Whatman #2 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried. The powder was dissolved in 50% glycerin to 2% (W/V) to prepare a solvent extract of mung bean seed, Manchurian birch sap, and Sori jaw root.

< Example 2> Nokdu seeds, Manchurian Birch Sap And fermentation extract production

After selecting 500 g of mung bean seeds and 500 g of sori chinensis roots as samples, they were sufficiently washed, and then the pulverized material was made fine so that it could pass through a 300 mesh sieve. 500 g of Manchurian birch sap was mixed thereto, and mung bean seeds, Sori birch root, and Manchurian birch sap were added to the culture solution of Saccharomyces genus (Saccharomyces cerevisiae KCTC 7904) so as to reach 10 g/L. Here, 1 to 4% of glucose is used as a carbon source, preferably 2% (W/V) is added, 0.1 to 1% of peptone is added, and preferably 0.5% (W/V) is additionally added to cultivate I did. The cultivation was carried out using a 5L fermenter at 37°C, maintaining the pH at 5-7, preferably maintaining the pH at 6, and incubating for 1 to 7 days, preferably for 5 days. After cultivation, the culture medium was centrifuged to remove the culture bacteria first.

The fermented product from which the culture bacteria were first removed was refluxed and extracted three times for 5 hours by adding 70% (V/V) ethanol aqueous solution, cooled, and then filtered through Whatman #2 filter paper. The filtered extract was transferred to a concentration tank, the filtered extract was concentrated under reduced pressure, and freeze-dried. This powder was dissolved in 50% glycerin to 2% (W/V) to prepare fermented extracts of mung bean seeds, Manchurian birch sap, and Sori jaw root.

< Example 3> Nokdu seeds, Manchurian Birch Sap And ultra-high pressure extract of Sori-jak root

After selecting 500 g of mung bean seeds and 500 g of sori birch root as samples, washing them sufficiently, and then adding 500 g of Manchuria birch sap to the finely ground mung bean seeds and the pulverized product of Sori birch root so that they can pass through a 300 mesh sieve. Using a pressure of 1000 MPa, the temperature was raised to 50° C., treated for 24 hours, and then cooled to room temperature.

The mixed extract subjected to ultra-high pressure was added to a 70% (V/V) ethanol aqueous solution, extracted under reflux three times for 5 hours, cooled, and then filtered through Whatman #2 filter paper. When filtration was completed, in the present invention, the extract obtained in the extraction step was transferred to a concentration tank, the filtered extract was concentrated under reduced pressure, and freeze-dried. The freeze-dried product was dissolved to 2% (W/V) using 50% glycerin to prepare ultra-high pressure extracts of mung bean seeds, Manchurian birch sap, and Sori jaw root.

< Example 4> Nokdu seeds, Manchurian Birch Sap And screaming roots After fermentation Manufacture of ultra-high pressure extract

After selecting 500 g of mung bean seeds and 500 g of sori chinensis roots as samples, they were sufficiently washed, and then the pulverized material was made fine so that it could pass through a 300 mesh sieve. 500 g of Manchuria birch sap was added to the pulverized product, and 10 g/L was added to the culture solution of the strain of Saccharomyces genus (Saccharomyces cerevisiae KCTC 7904). Here, 1 to 4% of glucose is used as a carbon source, preferably 2% (W/V) is added, 0.1 to 1% of peptone is added, and preferably 0.5% (W/V) is further added to cultivate I did. The cultivation was carried out using a 5L fermenter at 37°C, maintaining the pH at 5-7, preferably maintaining the pH at 6, and incubating for 1 to 7 days, preferably for 5 days. After culturing the microorganisms, the culture solution was centrifuged to remove the culture bacteria first.

The fermented products of mung bean seed, Manchurian birch sap, and Sori-jak root from which the culture bacteria were first removed were heated to 50° C. under a pressure of 1000 MPa using an ultra-high pressure processor, and subjected to ultra-high pressure treatment for 24 hours, and then cooled to room temperature. The fermented product of ultra-high-pressure treated mung bean seed, Manchurian birch sap, and Sori jaw root was added to a 70% (V/V) ethanol aqueous solution, extracted with reflux 3 times for 5 hours, cooled, and then cooled, Whatman #2 filter paper Filtered. When filtration was completed, in the present invention, the extract obtained in the extraction step was transferred to a concentration tank, concentrated under reduced pressure at 50° C. or lower, and then freeze-dried. Concentration under reduced pressure was performed using a rotary vacuum concentrator, and concentration conditions were concentrated under reduced pressure for 2 hours while rotating at 50-70°C and 600-1200 rpm at a pressure of 650 mmHg-750 mmHg into a 1,000 ml round flask with 700 ml of extract. I did. The concentrated solution concentrated under reduced pressure is pre-frozen, lowered the temperature to -40°C, maintained at -40°C for 2 to 4 hours, then dropped to -80°C through trap freezing, and the vacuum is maintained at 10 to 30 mTorr. Rise again to -40°C over 120 minutes. Thereafter, it was maintained at -20°C for 240 minutes, 0°C for 40 minutes, 15°C for 340 minutes, and 30°C for 130 minutes, and maintained at 1300 minutes until completely dried. The freeze-dried product was dissolved to 2% using 50% glycerin to prepare fermented ultra-high pressure extracts of mung bean seed, Manchurian birch sap, and Sori jaw root.

< Comparative Example 1> Preparation of Mung Bean Seed Solvent Extract

1000 g of green beans seeds were selected as a sample, and a solvent extract of green beans seeds was prepared according to the method of Example 1.

< Comparative Example 2> Manchurian Birch Sap Preparation of solvent extract

1000 g of Manchurian birch sap was selected as a sample, and a solvent extract of Manchurian birch sap was prepared according to the method of Example 1.

< Comparative Example 3> Preparation of Solvent Extract

1000 g of Sori Trang root was selected as a sample, and a solvent extract of Sori Trang root was prepared according to the method of Example 1.

< Manufacturing example 1> Cosmetic Produce

A cosmetic composition containing the mixed extract of mung bean seed, Manchurian birch sap, and Sori jaw root of the present invention was prepared as shown in Table 1 below. Formulation Examples 1 to 4 are the solvent extracts of mung bean seeds, Manchuria birch sap and Sori Tzu root of Example 1, mung bean seeds of Example 2, fermented extract of Manchurian birch sap and Sori Tzu root, and mung beans of Example 3, respectively. Seeds, Manchuria birch sap and ultra-high pressure extract of Sorijak root, Mungbean seed of Example 4, Manchuria birch sap, and fermented ultra-high pressure extract of Sori’s root were contained as active ingredients, Comparative Formulation Example 1 was Mungbean seed and Manchuria jasmine It is a cosmetic formulation that does not contain any tree sap or sori chinensis root. And Comparative Formulation Examples 2 to 4 are examples of formulations containing Mung bean seeds, Manchurian birch sap, and Comparative Examples 2 to 4 obtained by solvent-extracting each of the sori jaw roots.

Ingredients (unit: weight %) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Glyceryl stearate SE 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Stearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 lanolin 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Polysorbate 60 1.3 1.3 1.3 1.3 1.3 1.3 1.3 1.3 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Hydrogenated vegetable oil 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Mineral oil 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoine 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Tocopherol acetate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Carboxyvinyl polymer 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 glycerin 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 1.3-butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Sodium hyaluronate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Triethanolamine 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 Methylparaben 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 incense 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Example 1 3 - - - - - - - Example 2 - 3 - - - - - - Example 3 - - 3 - - - - - Example 4 - - - 3 - - - - Comparative Example 1 - - - - - 3 - - Comparative Example 2 - - - - - - 3 Comparative Example 3 - - - - - - 3 Distilled water Balance Balance Balance Balance Balance Balance Balance Balance Sum 100 100 100 100 100 100 100 100

< Experimental example 1> Measurement of skin moisturizing enhancement effect

The cosmetic formulations of Examples 1 to 4 (Formulation Examples 1 to 4) and Comparative Formulation Examples 1 to 4 were used to compare the effect of promoting skin moisturization.

The experiment was divided into two groups of 20 people per group for 40 people in their 20s and 40s without skin disease, and the creams of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 were applied twice daily for 1 month on the face and before each group. It was applied to the thin part. Measure the skin conductivity in a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under constant temperature and humidity conditions (24℃, humidity 40%) before starting the application, and take the basic value, and the skin conductivity after 1, 2, 4 weeks Was measured to evaluate the increase rate. The results are shown in Table 2 below.

sample 1 week later After two weeks 4 weeks later Formulation Example 1 25 26 27 Formulation Example 2 39 37 39 Formulation Example 3 32 32 33 Formulation Example 4 44 45 46 Comparative Formulation Example 1 13 14 17 Comparative Formulation Example 2 20 21 22 Comparative Formulation Example 3 21 20 23 Comparative Formulation Example 4 20 19 19

As can be seen from the results of Table 2, formulation examples 1 to 4 containing the mixed extract of mung bean seed, Manchurian birch sap, and Sori Trang root of the present invention are mung bean seed, Manchurian birch sap, Sori Trang root Compared to Comparative Formulation Example 1, which did not contain the mixed extract of No. 2 and Comparative Formulation Examples 2 to 4 containing only extracts of Mung bean seed, Manchuria birch sap, and Sori-gwi, the rate of increase in skin conductivity was excellent.

In particular, the formulations containing the extracts (Formulation Examples 2 to 4) obtained by fermentation extraction, ultra-high pressure extraction, or ultra-high pressure extraction after fermentation were superior to the mixed extract obtained by the solvent extraction method (Formulation Example 1), and after fermentation Formulation Example 4 containing the extract subjected to ultra-high pressure extraction had the best skin conductivity.

In addition, in general, since the skin conductivity is proportional to the amount of skin moisture, formulation examples 1 to 4 containing the mixed extract of mung bean seed, Manchurian birch sap, and Sori birch root of the present invention from the above results are And compared to Comparative Formulation Example 1 that does not contain the mixed extract of Sori Trang root, and Comparative Formulation Examples 2 to 4 containing a single extract of Mung bean seed, Manchurian birch sap, Sori Trang root, it was also maintained that the skin moisture content was higher. I can confirm.

From these results, it can be seen that the mixed extract of mung bean seed, Manchurian birch sap, and sori birch root is effective in promoting skin moisturization. It can be seen that it has the most outstanding effect.

This is because a number of active ingredients including polyphenols and isoflavones among the constituents of mung bean seed, Manchurian birch sap, and Sori birch root extract were transformed or concentrated into more effective active ingredients through fermentation and ultra-high pressure extraction steps. Is estimated.

< Experimental example 2> Test of skin irritation relief effect by lactic acid

In order to find out the skin irritation relief effect by the mixed extract of mung bean seed, Manchurian birch sap, and Sori japonica root of the present invention, a skin irritation relief effect test by lactic acid was conducted, and the test method is as follows.

Fibroblasts, which are human skin cells (Korea Cell Line Bank, Republic of Korea) were cultured in a T-75 flask (Falcon, United States of America) until about 80% growth. This was transferred to a 96-well plate (Falcon, United States of America) at 3×10 ⁴ cells/well and cultured for 24 hours. After cultivation, it was confirmed through a microscope whether the cells were completely attached and grown well, and the experiment was conducted using lactic acid as a skin cell stimulator. At this time, 0.2% of lactic acid was used. That is, 200 µl of DMEM (Sigma, United States of America) medium containing 0.2% lactic acid was added to each of the 96-wells, and the mixed extracts of mung bean seed, Manchurian birch sap and Sori jaw root prepared in Examples 1 to 4 were added. Each was adjusted to 1.0% (v/v) and added. At this time, lactic acid and mung bean seed extract, Manchuria birch sap, and sori chinensis root extract were not added as a control group, and only lactic acid was added as a control group. In order to compare the viability of cells after 12 hours of addition of the test substance, MTT (Sigma, USA) solution (3mg/ml) was added and the cell viability was measured at 570nm using an ELISA READER (Molecular Devices, USA). And the cell viability (%) was calculated according to Equation 1 below, and the experimental results are shown in Table 3 below.

Test substance (concentration %) Cell viability (%) Control 100 Extract of Example 1 (1%) 99.7 Extract of Example 2 (1%) 99.7 Extract of Example 3 (1%) 99.8 Extract of Example 4 (1%) 99.9 Comparative group (lactic acid 0.2%) 0.0 Lactic acid (0.2%) + extract of Example 1 (1%) 66.7 Lactic acid (0.2%) + extract of Example 2 (1%) 77.1 Lactic acid (0.2%) + extract of Example 3 (1%) 76.5 Lactic acid (0.2%) + extract of Example 4 (1%) 81.2

As can be seen from the results of Table 3, the addition of the mung bean seed extract, Manchurian birch sap, and the sori jaw root extract showed the alleviating effect of cell generation inhibition by lactic acid treatment. In addition, each extract itself did not have any effect on the cell viability, so it was found that there was no cytotoxicity.

< Experimental example 3> MMP -1 Production inhibition effect-Wrinkle improvement effect

Fibroblasts (Korea Cell Line Bank, Republic of Korea), which are human normal skin cells, were inoculated into 48-well microplates (Nunc, Denmark) at 1×10 ⁶ cells per well, and DMEM medium (Sigma, United States of America) and conditions of 37°C Serum-free DMEM medium added so that the final concentration of the mixed extracts of Examples 1 and 4 was 1.0% after incubation for 24 hours, and radish that did not contain mung bean seed extract, Manchurian birch sap, and Sori japonica root extract as a control. It was further incubated for 48 hours in serum DMEM medium. After incubation, the supernatant of each well was collected and the amount of newly synthesized MMP-1 (ng/ml) was measured using an MMP-1 assay kit (Amersham, United States of America), and MMP-1 was generated according to Equation 2 below. The inhibition rate was calculated, and the results are shown in Table 4. At this time, TGF-β (10 ng/ml, Roche, United States of America) was used as a positive control for the inhibition rate of MMP-1 production.

Test substance (concentration %) MMP-1 production inhibition rate (%) TGF-β (10 ng/ml) 76.8 Example 1 (1%) 78.8 Example 2 (1%) 82.2 Example 3 (1%) 81.3 Example 4 (1%) 84.9 Extract of Comparative Example 1 (1%) 76.2 Extract of Comparative Example 2 (1%) 76.3 Extract of Comparative Example 3 (1%) 76.2

As shown in the results of Table 4, with respect to the MMP-1 production inhibition rate, the group treated with the mixed extracts of Mung bean seeds, Manchurian birch sap, and Sori jaw root of Examples 1 to 4 at a concentration of 1.0% was a positive control group. Compared to the TGF-β treatment group used as and Comparative Formulation Examples 2 to 4, it showed a better inhibition rate of MMP-1 production. Here, MMP-1 is a collagen-degrading enzyme, and is a protein whose expression is increased by stress generated by various external stimuli (ultraviolet rays). When UV rays are applied, its synthesis is increased in skin cells, it acts to dissolve and decompose matrix proteins in the skin, and external stimuli, including UV rays, further increase the synthesis of MMP-1 enzyme and promote aging. The inhibition of the production of this MMP-1 enzyme means that it is effective in improving the wrinkles of the skin. Therefore, the mixed extract of mung bean seed, manchurian birch sap and sori birch root reduces the synthesis of this protein, which means that it solves skin problems caused by stress, etc. and is effective in improving wrinkles.

Hereinafter, based on the results of the above-described experimental examples, a cosmetic composition including the mung bean seed extract, Manchurian birch sap, and the Sori chinensis root extract of the present invention is prepared and presented. However, it is not intended to limit the composition of the present invention to the following prescription examples.

Prescription example 1: flexible lotion

Among the cosmetics containing the mixed extract of mung bean seed, Manchurian birch sap, and Sori chinensis root, the formulation examples of flexible cosmetic water are as follows.

ingredient content(%) Mixed extract of mung bean seed, Manchurian birch sap, and sori chinensis root 1.0 glycerin 4.0 1.3-butylene glycol 1.0 Allantoin 0.1 DL-panthenol One E.D.T.A-2NA 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 Nicole HCO 60 0.4 Methyl paraben 0.2 incense 0.01 Distilled water Balance Sum 100

Prescription example 2: nourishing lotion

Among the cosmetics containing the mixed extract of Mung bean seed, Manchurian birch sap, and Sori chinensis root, prescription examples of nutritional cosmetic water are as follows.

ingredient Content (unit: wt%) Mixed extract of mung bean seed, Manchurian birch sap, and sori chinensis root 3.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hydrogenated vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 1.0 Tocopherol acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 6.0 1.3-butylene glycol 2.0 Sodium hyaluronate 1.0 Triethanolamine 0.12 Methylparaben 0.2 incense 0.02 Distilled water Balance Sum 100

Prescription example 3: nourishing cream

Among cosmetics containing mixed extracts of mung bean seed, Manchurian birch sap, and Sori chinensis root, a prescription example of a nutritional cream is as follows.

ingredient Content (unit: wt%) Mixed extract of mung bean seed, Manchurian birch sap, and sori chinensis root 5.0 Lipotype glycerin monostearate 1.0 Cetearyl alcohol 3.2 Stearic acid 2.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.5 Hydrogenated vegetable oil 1.0 Squalane 2.0 Mineral oil 4.0 Trioctanoine 7.0 Dimethicone 2.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 4.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Methylparaben 0.2 incense 0.1 Distilled water Balance Sum 100

As described above, specific parts of the present invention have been described in detail, and it is obvious that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereto for those of ordinary skill in the art. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (5)

A cosmetic composition for improving skin wrinkles, comprising a mixed extract of mung bean seed, Manchurian birch sap, and Sori jaw root, wherein the mixed extract is extracted by an ultra-high pressure treatment extraction method after fermentation. The cosmetic composition of claim 1, wherein the mixed extract contains 0.0001 to 30% by weight based on the total weight of the cosmetic. delete delete delete