CN108948227A - A kind of method that high-voltage pulse extracts okra pectin - Google Patents
A kind of method that high-voltage pulse extracts okra pectin Download PDFInfo
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- CN108948227A CN108948227A CN201810561573.XA CN201810561573A CN108948227A CN 108948227 A CN108948227 A CN 108948227A CN 201810561573 A CN201810561573 A CN 201810561573A CN 108948227 A CN108948227 A CN 108948227A
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- okra
- voltage pulse
- pectin
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- okra pectin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
- C08B37/0048—Processes of extraction from organic materials
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of methods that high-voltage pulse extracts okra pectin, it is related to okra pectin extraction technical field, the extracting method is using okra okra fruit as raw material, okra okra fruit is successively passed through into enzyme deactivation, enzymatic hydrolysis, high-pressure pulse electric processing and alcohol analysis processing, preparation method of the invention is simple, non-heat treated technology is belonged to using high-pressure pulse electric, plant cell membrane can successfully be destroyed, increase intracellular matter dissolution rate, have the advantages that improve recovery rate and keeps the stability of okra pectin.
Description
Technical field
The invention belongs to pectin extraction technical fields, are related to okra pectin extraction technical field, and in particular to Yi Zhonggao
The method for pressing DISCHARGE PULSES EXTRACTION okra pectin.
Background technique
Okra is 1 year draft plant, and nutritive value is high, comparable to ginseng.Okra various pieces all contain hemicellulose
Element, cellulose and lignin.Contain protein 2.5g, fat 0.1g, carbohydrate 2.7g, crude fibre in every 100g okra fruit
3.9g, retinol1 0.2mg, vitamin B2 0.06mg, vitamin C 44mg, vitamin e1 .03mg, nicotinic acid l.0mg, dimension
Raw element A660 international unit, mineral nutrition potassium 95mg, Ca45 mg, phosphorus 65mg, magnesium 29mg, okra okra fruit also contain by collagen egg
The viscous protein of white and mucopolysaccharide composition, and the Pectic polysaccharides substance being made of galactolipin, araban and pectin, these
Okra stickum and pectic substance have the resistance of enhancing body, promote the excretion of organic substance in body, reduce
The accumulation of toxin in vivo, reduces the effect of cholesterol level, with okra be worth exploitation, the research of okra pectin,
Also get more and more attention, at present the extracting method of pectin mainly have acid-hydrolysis method, ion-exchange-resin process, microorganism extraction method,
Microwave loss mechanisms, enzyme extraction method and ultrasonic extraction etc., as China Patent Publication No. CN106699917A discloses a kind of surpass
The extracting method of sound assisted extraction okra polysaccharide and pectin carries out hot water extraction using okra dry powder as raw material, and de- egg is concentrated
White okra pectin and polysaccharide compound with ethyl alcohol extraction system.But traditional pectin extraction method exists into mostly
Ben Taigao, recovery rate it is low and extract poor product quality the shortcomings that, therefore, the extraction process of okra pectin is improved, is ground
Study carefully a kind of low cost out, high yield, good product quality okra pectin extraction new process have and its great meaning.
Summary of the invention
According to the above-mentioned deficiencies of the prior art, the technical problem to be solved by the present invention is to propose that a kind of high-voltage pulse extracts
The method of okra pectin, in order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows:
A kind of method that high-voltage pulse extracts okra pectin, the specific steps are as follows:
1) stock up: take okra okra fruit stalk to clean up, carry out destroy the enzyme treatment, then wash 2-3 time except depigmentaton with
Sugar, deseeding dry, pulverize and sieve with 100 mesh sieve, and obtain okra fruit powder；
2) high-voltage pulse is handled: okra fruit powder being added containing in zyme extract, constant temperature stirring is extracted, and feed liquid is then adjusted
Than and pH value of solution, finally by solution adjusted carry out high-pressure pulse electric processing；
3) step (2) treated solution is subjected to centrifugal treating, ethanol solution is added in filtrate, stirred, stood,
Centrifugation, obtained sediment are washed 2-3 times with dehydrated alcohol, collect ethyl alcohol, and dry sediment obtains okra pectin.
Preferably, in order to save the energy, extraction efficiency is improved, the temperature of the destroy the enzyme treatment is 75-85 DEG C, and the time is
50-70s。
Preferably, described containing enzyme amount is contained in zyme extract for 7500-8000u/g, enzyme is the fruit that mass ratio is 1-2:1-1.5
Glue enzyme and cellulase mixture.
Preferably, okra fruit powder and the solid-liquid ratio containing zyme extract are 1:8-12 before the solid-liquid ratio adjusts, after adjustment
For 1:18-25.
Preferably, it is 6-7 before the pH value of solution adjustment, is 3-4 after adjustment, adjusts hydrochloric acid, 4- that liquid is concentration 10-15%
One of the sulfuric acid of 6mol/L, the nitric acid of 1.5-3mol/L or citric acid of 5-10%.
Preferably, the constant temperature stirring Extracting temperature is 35-45 DEG C, time 30-40min, revolving speed 20-30r/min.
Preferably, the electric field strength 10-20kv/cm, umber of pulse 5-10, temperature 55-65 of the high-pressure pulse electric processing
DEG C, high-pressure pulse electric belongs to non-heat treated technology, can successfully destroy plant cell membrane, increases intracellular matter dissolution rate.
Preferably, the revolving speed of the centrifugation is 4000-5000r/min, time 4-7min.
Preferably, the temperature of the dry sediment is 45-50 DEG C.
Compared with prior art, beneficial effects of the present invention:
1. the present invention carries out the extraction of okra pectin using enzyme additional high pressure impulse electric field, enzyme destroys okra okra fruit
Cell wall, high-pressure pulse electric belong to non-heat treated technology, can successfully destroy plant cell membrane, increase intracellular matter dissolution
Rate, the two, which combines, not only has the recovery rate for improving okra pectin, and the pectin quality extracted is high, pollution-free.
2. preparation method of the present invention is easy to operate, have the advantages that energy conservation and environmental protection, can sufficiently extract okra pectin energy again
The integrality for keeping the functional component of pectin, improves the value of okra.
Specific embodiment
It below by the description to embodiment, is described in further detail, to help those skilled in the art to this hair
Bright inventive concept, technical solution have more complete, accurate and deep understanding.
Embodiment 1
The method of the present embodiment mesohigh DISCHARGE PULSES EXTRACTION okra pectin, the specific steps are as follows:
1) it stocks up: taking the fresh disease-free stalk okra okra fruit of 1kg to clean up, okra okra fruit is placed at 85 DEG C
Carry out destroy the enzyme treatment 60s, then carry out washing 3 times except depigmentaton and sugar, after deseeding at 40 DEG C of temperature forced air drying to containing
Water 20% is crushed and is sieved with 100 mesh sieve, and okra fruit powder is obtained；
2) high-voltage pulse is handled: okra fruit powder being added and is mixed by the pectase and cellulase that mass ratio is 1.5:1
Be 8000u/g containing in zyme extract containing enzyme amount, and keeping solid-liquid ratio is 1:9, then pH of mixed will mix between 6-7
It closes liquid to be placed in 45 DEG C of constant temperature water bath, 35min is extracted in stirring after revolving speed 25r/min, and then plus water adjusting solid-liquid ratio is
1:23 and the citric acid solution of addition 8% adjust pH of mixed between 3-4, and solution adjusted is finally run through wriggling
Pump feed carries out high pressure by being set with electric field strength 15kv/cm, umber of pulse 8, the high-voltage pulse electric field device of temperature 60 C
Impulse electric field processing；
3) centrifugal treating is carried out in the centrifuge for being 5000r/min by step (2) treated solution is placed in setting speed
Filtrate, is added isometric ethanol solution, is sufficiently stirred by 5min, filtering, is stood, by alcohol analysis liquid in revolving speed 4000r/
It is centrifuged 4min under min, obtained sediment is washed 3 times with dehydrated alcohol, and it is repeatable that the ethanol solution of washing carries out recycling
It utilizes, sediment is dried under temperature 45 C again, obtains okra pectin.
Embodiment 2
The present embodiment is with embodiment 1, the difference is that the electric field strength 10kv/ of the present embodiment mesohigh impulse electric field processing
Cm, umber of pulse 5,55 DEG C of temperature, be 1:25 after solid-liquid ratio adjustment.
Embodiment 3
The present embodiment is with embodiment 1, the difference is that the electric field strength 20kv/ of the present embodiment mesohigh impulse electric field processing
Cm, umber of pulse 10,65 DEG C of temperature, be 1:18 after solid-liquid ratio adjustment.
Weighing is carried out to the okra pectin of embodiment 1-3 preparation and calculates recovery rate, recovery rate calculation formula=(okra
Pectin weigh/weighs okra okra fruit weight) × 100%, the recovery rate for being computed the okra pectin of embodiment 1-3 preparation exists
20.1%, wherein the recovery rate of embodiment 1 is up to 23.5%.
The okra of extraction is faint yellow solid, and free from extraneous odour is dissolved in 20 times of water in viscous liquid, takes dry pectin
Powder 1.0g is added purified water 50mL, is stirred continuously with glass bar, can be observed in glutinous thick liquid.
The present invention is exemplarily described above in conjunction with specific embodiment, it is clear that the present invention implements not by upper
The limitation of mode is stated, if the improvement for the various unsubstantialities that the inventive concept and technical scheme of the present invention carry out is used, or
It is not improved the conception and technical scheme of the invention are directly applied to other occasions, protection scope of the present invention it
It is interior.Protection scope of the present invention should be determined by the scope of protection defined in the claims.
Claims (9)
1. a kind of method that high-voltage pulse extracts okra pectin, which is characterized in that specific step is as follows:
1) it stocks up: okra okra fruit stalk being taken to clean up, carry out destroy the enzyme treatment, then wash 2-3 times and remove depigmentaton and sugar,
Deseeding dry, pulverize and sieve with 100 mesh sieve, and obtain okra fruit powder；
2) high-voltage pulse is handled: okra fruit powder is added containing in zyme extract, constant temperature stirring is extracted, then adjust solid-liquid ratio and
Solution adjusted is finally carried out high-pressure pulse electric processing by pH value of solution；
3) step (2) treated solution is subjected to centrifugal treating, ethanol solution is added in filtrate, stirred, stood, from
The heart, obtained sediment are washed 2-3 times with dehydrated alcohol, collect ethyl alcohol, and dry sediment obtains okra pectin.
2. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that the destroy the enzyme treatment
Temperature be 75-85 DEG C, time 50-70s.
3. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that described to be extracted containing enzyme
Containing enzyme amount in liquid is 7500-8000u/g, and enzyme is the pectase and cellulase mixture that mass ratio is 1-2:1-1.5.
4. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that the solid-liquid ratio tune
Whole preceding okra fruit powder and the solid-liquid ratio containing zyme extract are 1:8-12, are 1:18-25 after adjustment.
5. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that the pH value of solution tune
It is 6-7 before whole, is 3-4 after adjustment, adjusting liquid is the nitre of the hydrochloric acid of concentration 10-15%, the sulfuric acid of 4-6mol/L, 1.5-3mol/L
One of acid or the citric acid of 5-10%.
6. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that the constant temperature stirring
Extracting temperature is 35-45 DEG C, time 30-40min, revolving speed 20-30r/min.
7. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that the high-voltage pulse
The electric field strength 10-20kv/cm of electric field treatment, umber of pulse 5-10,55-65 DEG C of temperature.
8. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that the centrifugation turns
Speed is 4000-5000r/min, time 4-7min.
9. the method that high-voltage pulse according to claim 1 extracts okra pectin, which is characterized in that described drying precipitated
The temperature of object is 45-50 DEG C.
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Cited By (5)
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| CN109619541A (en) * | 2018-12-10 | 2019-04-16 | 静宁县金果实业有限公司 | A method of edible apple pectin is produced using apple pomace |
| CN109879985A (en) * | 2019-03-20 | 2019-06-14 | 广州市莱檬生物科技有限公司 | A kind of preparation method of medicinal small molecule pectin |
| CN110240663A (en) * | 2019-06-06 | 2019-09-17 | 华南理工大学 | A kind of method that impulse electric field collaboration cellulase extracts pomelo-pectin |
| CN111454380A (en) * | 2020-03-18 | 2020-07-28 | 杭州师范大学 | Method for efficiently extracting okra pectin |
| CN114957501A (en) * | 2022-06-09 | 2022-08-30 | 南京泛成生物科技有限公司 | Method for improving extraction rate and quality of locust bean gum |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109619541A (en) * | 2018-12-10 | 2019-04-16 | 静宁县金果实业有限公司 | A method of edible apple pectin is produced using apple pomace |
| CN109879985A (en) * | 2019-03-20 | 2019-06-14 | 广州市莱檬生物科技有限公司 | A kind of preparation method of medicinal small molecule pectin |
| CN109879985B (en) * | 2019-03-20 | 2021-03-19 | 广州市莱檬生物科技有限公司 | Preparation method of medicinal micromolecule pectin |
| CN110240663A (en) * | 2019-06-06 | 2019-09-17 | 华南理工大学 | A kind of method that impulse electric field collaboration cellulase extracts pomelo-pectin |
| CN110240663B (en) * | 2019-06-06 | 2021-12-17 | 华南理工大学 | Method for extracting pomelo peel pectin by using pulsed electric field and cellulase |
| CN111454380A (en) * | 2020-03-18 | 2020-07-28 | 杭州师范大学 | Method for efficiently extracting okra pectin |
| CN114957501A (en) * | 2022-06-09 | 2022-08-30 | 南京泛成生物科技有限公司 | Method for improving extraction rate and quality of locust bean gum |
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Application publication date: 20181207 |