CN112442136A - Method for extracting functional components from tremella - Google Patents
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- CN112442136A CN112442136A CN201910829061.1A CN201910829061A CN112442136A CN 112442136 A CN112442136 A CN 112442136A CN 201910829061 A CN201910829061 A CN 201910829061A CN 112442136 A CN112442136 A CN 112442136A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
Abstract
The invention discloses a method for extracting functional components from tremella, which comprises the steps of leaching tremella with ethanol water solution, centrifuging, concentrating and drying to obtain flavonoid compounds; and performing enzymolysis and filtration on the tremella residue obtained after centrifugation to obtain a mixed solution containing a small amount of micromolecular polysaccharide, protein and poly-acetylglucosamine and the wall-broken tremella residue. Leaching the Tremella residue twice with buffer aqueous solution, filtering, removing protein from the filtrate under the action of complex enzyme, removing inorganic salt with semipermeable membrane, concentrating with ultrafiltration membrane, and precipitating with ethanol to obtain chitosan and Tremella polysaccharide. The invention has simple process, mainly adopts bio-enzyme action technology, has mild process conditions, obtains flavonoid compounds, the polyacetyl glucosamine and the tremella polysaccharide by sectional extraction, has various functional components of the tremella and wide application range, fully and effectively utilizes the tremella, and is beneficial to the full development of the resource of the tremella.
Description
Technical Field
The invention belongs to the technical field of edible fungus deep processing, and particularly relates to a method for extracting functional components of tremella.
Background
Tremella is also known as Tremella, and belongs to fungi, and is called "crown of fungi". Tremella fuciformis is sweet and light in taste, mild in nature and nontoxic, has the effects of tonifying spleen and promoting appetite, and also has the effects of benefiting qi, clearing intestines, nourishing yin and moistening lung. It can not only enhance immunity, but also enhance tolerance of tumor patients to radiotherapy and chemotherapy.
Flavonoid compounds in Tremella can be added into food and medicine. The chitosan in the tremella can play a role in biological barriers such as lubrication, isolation and the like on skin, wound surfaces and mucous membranes, can promote wound surface healing and reduce exudation, and is favorable for promoting blood cell aggregation. The polysaccharide in the tremella has the health-care functions of improving the immunity of organisms, resisting viruses, resisting tumors, reducing blood sugar and the like, has the effects of beautifying and tendering skin, and has a good protection effect on the skin. The tremella has abundant functional components, so that the tremella gradually becomes a research hotspot. However, tremella is mainly eaten in China, and compared with developed countries, deep processing of tremella in China starts to be late. The extraction of the functional components of the tremella has the problems of incomplete extraction components, complex extraction process, low extraction rate, high extraction cost and the like, and is not beneficial to the full development and utilization of the tremella.
The full and effective extraction of the functional components of the tremella has great significance for the reasonable utilization of tremella resources. At present, the extraction method of the tremella as the edible fungus material only aims at one component, cannot fully utilize the raw materials, and does not extract and separate the flavonoid compound and the chitosan of the tremella for utilization.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the method for extracting the functional ingredients of the tremella, which can carry out multi-level extraction on the functional ingredients of the tremella and fully utilize raw materials. The method effectively extracts flavonoid compounds in the tremella fuciformis, separates glucosamine independently, and can provide possibility for other applications, and the tremella fuciformis polysaccharide obtained by separating and extracting the flavonoid compounds and the glucosamine in the previous process has higher content and better product stability, and better meets the market demand.
In order to solve the problems, the technical scheme provided by the invention is as follows:
s1, removing roots of fresh tremella, rinsing with clear water, draining, putting a proper amount of tremella into an extraction tank, adding 95% ethanol by volume, wherein the volume of the 95% ethanol is 2-5 times of the mass of tremella, heating to 50-65 ℃, leaching for 2-6 hours, shearing and crushing for 30-90 min by using a dispersion disc, stirring for 6-12h at room temperature, and performing centrifugal separation at the rotating speed of 400-1000r/min to obtain tremella filter residues and yellow ethanol feed liquid; washing the white fungus filter residue with water for later use;
s2, precisely filtering the yellow ethanol feed liquid obtained in the step S1, concentrating the filtrate under reduced pressure, recovering ethanol to be viscous, adding 1-3 times of purified water into the viscous ethanol residue, heating to 50 ℃, filtering, washing a filter cake for 2-6 times by using ice water at 0 ℃, and then drying in vacuum to obtain the flavonoid compound;
s3, adding pectinase and acid cellulase in a weight ratio of 1:3 into the tremella filter residue obtained in the step S1 for enzymolysis, heating to 40 ℃, slowly heating to 1 ℃ every 10min, heating to 45-60 ℃ required by the enzymolysis, keeping the temperature for 30-60min, inactivating the enzyme, and filtering with a 400-mesh nylon gauze to obtain a mixed solution containing a small amount of micromolecule polysaccharide, protein and poly (acetylamino polysaccharide) and tremella residue subjected to enzymolysis wall breaking;
s4, adding a buffer aqueous solution which is 5-50 times of the weight of the tremella residues into the tremella residues obtained in the step S3, leaching, heating to 80 ℃, slowly heating, raising the temperature by 1 ℃ every 10min, keeping the temperature for 6-8 hours after the temperature reaches 95 ℃, filtering with a 400-mesh gauze while the temperature is hot, leaching twice repeatedly, and collecting filtrate for later use;
s5, mixing the mixed solution obtained in S3 and the filtrate obtained in S4, adjusting the pH value to 5.0-6.5, adding a proper amount of complex enzyme at the temperature of 50-60 ℃, preserving the heat for 2 hours, inactivating the enzyme, filtering the mixture by using 2500-mesh 3000-mesh polypropylene fiber cloth to obtain filtrate, removing inorganic salt from the filtrate through a semipermeable membrane, performing ultrafiltration concentration by using an ultrafiltration membrane with the pore diameter of 50-500A DEG until the content of soluble solid is 0.5-1.0%, adding 5-20 times of anhydrous ethanol, uniformly stirring, cooling to 0-4 ℃ to obtain an alcohol precipitation feed liquid;
s6, performing graded filtration on the alcohol precipitation liquid obtained in the step S5 by using 8000-10000-mesh polypropylene fiber cloth and filter paper with the aperture of 1-11 mu m, washing filter residues by using 5-15 times of absolute ethyl alcohol, and then performing vacuum drying to obtain light yellow polyacetyl glucosamine; vacuum concentrating the filtrate until the content of soluble solid is 3.0% -6.0%, adding anhydrous ethanol, standing for 24h to obtain flocculent precipitate, centrifuging, washing the filter cake with anhydrous ethanol, and vacuum drying to obtain Tremella polysaccharide.
The invention adopts ethanol water solution to leach, centrifuge, concentrate and dry the tremella to obtain the tremella flavonoid compound, which can be widely applied to food, medicine and cosmetics. The wall breaking treatment is carried out on the cell wall of the tremella fuciformis under the action of the biological enzyme, the impurity removal is carried out on the extracting solution, the conditions in the process are mild, and the loss of target components can be reduced. The chitosan and the tremella polysaccharide are obtained by leaching the buffer aqueous solution, and the target components can be extracted to the maximum extent under mild conditions.
Preferably, in the step S1, the stirring time at room temperature is 10 hours, the centrifugal rotation speed is 600r/min, and the centrifugal time is 60 min; the filter cake was washed with ice water at 0 ℃ until no ethanol taste was observed.
Preferably, the amount of the pectinase and the acid cellulase added in the step S3 is 0.5-1.5 per mill of the weight of the fresh tremella, the enzymolysis temperature is 50 ℃, and the enzymolysis medium is purified water with the pH value of 3.0-6.0 which is 5-10 times of the mass of the fresh tremella.
Preferably, the aqueous buffer solution in step S4 is an aqueous buffer solution composed of inorganic salts and organic salts and having a pH of 8-10, and the amount of the aqueous buffer solution added is 20 times of that of the white fungus residue.
Preferably, the complex enzyme in the step S5 is galacturonase and protease, and is obtained by mixing the components in a mass ratio of 1:2-1:5, and the addition amount of the complex enzyme is 0.3-1 per mill of the mixed liquid obtained in the step S3 and the filtrate obtained in the step S4.
Preferably, in the step S5, the polypropylene fiber cloth is 2500 meshes in specification, and the pore size of the ultrafiltration membrane is 50 to 100A °; the appropriate amount of absolute ethanol means 15 times the volume of the concentrate.
Preferably, in the step S6, the mesh number of the polypropylene fiber cloth is 10000 meshes, and the addition amount of the appropriate amount of the absolute ethyl alcohol is 10 times of the volume of the concentrated solution.
Compared with the prior art, the method has the advantages that the process is simple, the process conditions are mild, the flavonoid compound, the chitosan and the tremella polysaccharide are obtained by sectional extraction, the obtained tremella has various functional components and wide application range, the tremella is fully and effectively utilized, and the resource of the tremella is fully developed.
Detailed Description
The present invention will be described in further detail with reference to the following examples:
the tremella fuciformis was purchased from Fujian, the reagents used were commercially available analytical pure products, and water was purified water.
Example 1:
a method for extracting functional components from Tremella comprises the following steps:
(1) extraction of flavonoids
S1, removing roots of commercially available fresh tremella, rinsing with clear water, draining, taking 1 kg of tremella, putting the tremella into an extraction tank, adding 2L of 95% ethanol, heating to 50-65 ℃, leaching for 2-6 hours, shearing and smashing by a dispersion disc for 60min, stirring at room temperature for 6h, and performing centrifugal separation at a rotating speed of 600r/min to obtain tremella filter residues and yellow ethanol feed liquid. Washing the white fungus filter residue with water for later use;
s2, precisely filtering the yellow ethanol feed liquid, concentrating the filtrate under reduced pressure, recovering ethanol to be viscous, adding 2 times of purified water into the viscous ethanol residue, heating to 50 ℃, filtering, washing a filter cake with 0 ℃ ice water until no ethanol smell exists, and drying the filter cake in vacuum to obtain a flavonoid compound, wherein the content is 58.3% as measured by an ultraviolet-visible spectrophotometry;
(2) extraction of polysaccharide from polyacetyl glucosamine and Tremella
And S3, adding pectinase and acid cellulase in a weight ratio of 1:3 into the tremella filter residue obtained in the step S1 for enzymolysis, wherein the adding amount is 0.5 per thousand of the weight of the fresh tremella, namely 0.5 g. Slowly heating to 40 deg.C, increasing temperature by 1 deg.C every 10min to 45-60 deg.C required for enzymolysis, keeping the temperature for 30-60min, inactivating enzyme, and filtering with nylon gauze to obtain mixed solution containing small amount of small molecular polysaccharide, protein and polyacetylaminopolysaccharide and Tremella residue broken by enzymolysis;
s4, adding the tremella residue obtained in the step S3 into a buffer aqueous solution with the mass 20 times that of the tremella residue for leaching, heating to 80 ℃, slowly heating, raising the temperature by 1 ℃ every 10min, keeping the temperature for 6 hours after the temperature reaches 95 ℃, filtering with a 400-mesh gauze while the temperature is hot, repeating leaching twice, and collecting filtrate for later use;
s5, mixing the mixed liquor obtained in the step S3 and the filtrate obtained in the step S4, adjusting the pH to 5.0-6.5, adding complex enzyme with the mass of 0.5 per thousand of the feed liquid at the temperature of 50-60 ℃, preserving heat for 2 hours, inactivating the enzyme, filtering with 2500-mesh polypropylene fiber cloth to obtain filtrate, removing inorganic salt from the filtrate through a semipermeable membrane, performing ultrafiltration concentration by using an ultrafiltration membrane with the pore diameter of 50-100A degrees until the content of soluble solids is 0.5-1.0%, adding 10 times of volume of absolute ethyl alcohol, uniformly stirring, cooling to 0-4 ℃ to obtain an alcohol precipitation feed liquid.
S6, filtering the alcohol precipitation liquid in the S5 by using 8000-mesh polypropylene fiber cloth and filter paper with the aperture of 3-5 mu m in a grading manner, washing filter residues by using 10 times of absolute ethyl alcohol, and then drying in vacuum to obtain light yellow polyacetyl glucosamine, wherein the content is 80.59% by a colorimetric method. Vacuum concentrating the filtrate until the content of soluble solid is 3.0% -6.0%, adding anhydrous ethanol, standing for 24 hr to obtain flocculent precipitate, centrifuging, washing the filter cake with anhydrous ethanol, vacuum drying, and pulverizing to obtain Tremella polysaccharide with content of 97.5% by sulfuric acid-phenol method.
Example 2:
a method for extracting functional components from Tremella comprises the following steps:
(1) extraction of flavonoids
S1, removing roots of commercially available fresh tremella, rinsing with clear water, draining, taking 1 kg of tremella, putting the tremella into an extraction tank, adding 4L of 95% ethanol, heating to 50-65 ℃, leaching for 2-6 hours, shearing and smashing by a dispersion disc for 60min, stirring at room temperature for 6h, and performing centrifugal separation at a rotating speed of 1000r/min to obtain tremella filter residues and yellow ethanol feed liquid. Washing the white fungus filter residue with water for later use;
s2, precisely filtering the yellow ethanol feed liquid, concentrating the filtrate under reduced pressure, recovering ethanol to be viscous, adding 2 times of purified water into the viscous ethanol residue, heating to 50 ℃, filtering, washing a filter cake with 0 ℃ ice water until no ethanol taste exists, drying the filter cake in vacuum to obtain a flavonoid compound, wherein the content is 62.7% as measured by an ultraviolet-visible spectrophotometry;
(2) extraction of polysaccharide from polyacetyl glucosamine and Tremella
And S3, adding pectinase and acid cellulase in a weight ratio of 1:3 into the tremella filter residue obtained in the step S1 for enzymolysis, wherein the adding amount is 1.0 per thousand of the weight of the fresh tremella, namely 1.0 g. Slowly heating to 40 deg.C, increasing temperature by 1 deg.C every 10min to 45-60 deg.C required for enzymolysis, keeping the temperature for 30-60min, inactivating enzyme, and filtering with nylon gauze to obtain mixed solution containing small amount of small molecular polysaccharide, protein and polyacetylaminopolysaccharide and Tremella residue broken by enzymolysis;
s4, adding the obtained tremella residues into a buffer aqueous solution with the mass 20 times that of the tremella residues for leaching, heating to 80 ℃, slowly heating, raising the temperature by 1 ℃ every 10min, keeping the temperature for 8 hours after the temperature reaches 95 ℃, filtering with a 400-mesh gauze when the temperature is hot, repeating leaching twice, and collecting filtrate for later use;
s5, mixing the mixed liquor obtained in the step S3 with the filtrate obtained in the step S4, adjusting the pH to 5.0-6.5, adding complex enzyme with the mass of 0.5 per thousand of the feed liquid at the temperature of 50-60 ℃, preserving heat for 2 hours, inactivating the enzyme, filtering with 3000-mesh polypropylene fiber cloth to obtain filtrate, removing inorganic salt from the filtrate through a semipermeable membrane, performing ultrafiltration concentration by using an ultrafiltration membrane with the pore diameter of 50-100A degrees until the content of soluble solids is 0.5-1.0%, adding 15 times of volume of absolute ethyl alcohol, uniformly stirring, cooling to 0-4 ℃ to obtain alcohol precipitation feed liquid;
s6, filtering the alcohol precipitation liquid in the S5 by using 8000-mesh polypropylene fiber cloth and filter paper with the aperture of 3-5 mu m in a grading manner, washing filter residues by using 5 times of absolute ethyl alcohol, and then drying in vacuum to obtain light yellow polyacetyl glucosamine, wherein the content is 81.89% by a colorimetric method. Vacuum concentrating the filtrate until the content of soluble solid is 3.0% -6.0%, adding anhydrous ethanol, standing for 24 hr to obtain flocculent precipitate, centrifuging, washing the filter cake with anhydrous ethanol, vacuum drying, and pulverizing to obtain Tremella polysaccharide with content of 98.9% by sulfuric acid-phenol method.
Example 3:
a method for extracting functional components from Tremella comprises the following steps:
(1) extraction of flavonoids
S1, removing roots of commercially available fresh tremella fuciformis, rinsing with clear water, draining, taking 0.5 kg of tremella fuciformis, putting into an extraction tank, adding 2L of 95% ethanol, heating to 50-65 ℃, leaching for 2-6 hours, shearing and smashing by a dispersion disc for 60min, stirring for 6h at room temperature, and performing centrifugal separation at a rotating speed of 1000r/min to obtain tremella fuciformis filter residues and yellow ethanol feed liquid. Washing the white fungus filter residue with water for later use;
s2, precisely filtering the yellow ethanol feed liquid, concentrating the filtrate under reduced pressure, recovering ethanol to be viscous, adding 2 times of purified water into the viscous ethanol residue, heating to 50 ℃, filtering, washing a filter cake with 0 ℃ ice water until no ethanol smell exists, and drying the filter cake in vacuum to obtain a flavonoid compound, wherein the content is 61.2% by ultraviolet visible spectrophotometry;
(2) extraction of polysaccharide from polyacetyl glucosamine and Tremella
And S3, adding pectinase and acid cellulase in a weight ratio of 1:3 into the tremella filter residue obtained in the step S1 for enzymolysis, wherein the adding amount is 1.0 per thousand of the weight of the fresh tremella, namely 0.5 g. Slowly heating to 40 deg.C, increasing temperature by 1 deg.C every 10min to 45-60 deg.C required for enzymolysis, keeping the temperature for 30-60min, inactivating enzyme, and filtering with nylon gauze to obtain mixed solution containing small amount of small molecular polysaccharide, protein and polyacetylaminopolysaccharide and Tremella residue broken by enzymolysis;
s4, adding the obtained tremella residues into a buffer aqueous solution with the mass 20 times that of the tremella residues for leaching, heating to 80 ℃, slowly heating, raising the temperature by 1 ℃ every 10min, keeping the temperature for 8 hours after the temperature reaches 95 ℃, filtering with a 400-mesh gauze when the temperature is hot, repeating leaching twice, and collecting filtrate for later use;
s5, mixing the mixed solution obtained in the S3 and the filtrate obtained in the S4, adjusting the pH to 5.0-6.5, adding complex enzyme with the mass of 0.5 per thousand of the feed liquid at the temperature of 50-60 ℃, preserving the heat for 2 hours, filtering the mixture by using 3000-mesh polypropylene fiber cloth after enzyme inactivation to obtain filtrate, removing inorganic salt from the filtrate by using a semipermeable membrane, performing ultrafiltration concentration by using an ultrafiltration membrane with the pore diameter of 50-100A DEG until the content of soluble solids is 0.5-1.0%, adding 15 times of volume of absolute ethyl alcohol, uniformly stirring, cooling to 0-4 ℃ to obtain alcohol precipitation feed liquid;
s6, carrying out graded filtration on the alcohol precipitation liquid in the S5 by using 10000-mesh polypropylene fiber cloth and filter paper with the aperture of 3-5 mu m, washing filter residues by using 5 times of absolute ethyl alcohol, and then drying in vacuum to obtain light yellow polyacetyl glucosamine, wherein the content is 82.3% by a colorimetric method. Vacuum concentrating the filtrate until the content of soluble solid is 3.0% -6.0%, adding anhydrous ethanol, standing for 24 hr to obtain flocculent precipitate, centrifuging, washing the filter cake with anhydrous ethanol, vacuum drying, and pulverizing to obtain Tremella polysaccharide with content of 98.3% by sulfuric acid-phenol method.
It should be noted that the above-mentioned embodiments illustrate rather than limit the scope of the invention, and that those skilled in the art will be able to modify the invention in its various equivalent forms after reading the present disclosure without departing from the scope of the invention as defined by the appended claims.
Claims (7)
1. A method for extracting functional components from tremella is characterized by comprising the following steps:
s1, removing roots of fresh tremella, rinsing with clear water, draining, putting a proper amount of tremella into an extraction tank, adding 95% ethanol by volume, wherein the volume of the 95% ethanol is 2-5 times of the mass of tremella, heating to 50-65 ℃, leaching for 2-6 hours, shearing and crushing for 30-90 min by using a dispersion disc, stirring for 6-12h at room temperature, and performing centrifugal separation at the rotating speed of 400-1000r/min to obtain tremella filter residues and yellow ethanol feed liquid; washing the white fungus filter residue with water for later use;
s2, precisely filtering the yellow ethanol feed liquid obtained in the step S1, concentrating the filtrate under reduced pressure, recovering ethanol to be viscous, adding 1-3 times of purified water into the viscous ethanol residue, heating to 50 ℃, filtering, washing a filter cake for 2-6 times by using ice water at 0 ℃, and then drying in vacuum to obtain the flavonoid compound;
s3, adding pectinase and acid cellulase in a weight ratio of 1:3 into the tremella filter residue obtained in the step S1 for enzymolysis, heating to 40 ℃, slowly heating to 1 ℃ every 10min, heating to 45-60 ℃ required by the enzymolysis, keeping the temperature for 30-60min, inactivating the enzyme, and filtering with a 400-mesh nylon gauze to obtain a mixed solution containing a small amount of micromolecule polysaccharide, protein and poly (acetylamino polysaccharide) and tremella residue subjected to enzymolysis wall breaking;
s4, adding a buffer aqueous solution which is 5-50 times of the weight of the tremella residues into the tremella residues obtained in the step S3, leaching, heating to 80 ℃, slowly heating, raising the temperature by 1 ℃ every 10min, keeping the temperature for 6-8 hours after the temperature reaches 95 ℃, filtering with a 400-mesh gauze while the temperature is hot, leaching twice repeatedly, and collecting filtrate for later use;
s5, mixing the mixed solution obtained in S3 and the filtrate obtained in S4, adjusting the pH value to 5.0-6.5, adding a proper amount of complex enzyme at the temperature of 50-60 ℃, preserving the heat for 2 hours, inactivating the enzyme, filtering the mixture by using 2500-mesh 3000-mesh polypropylene fiber cloth to obtain filtrate, removing inorganic salt from the filtrate through a semipermeable membrane, performing ultrafiltration concentration by using an ultrafiltration membrane with the pore diameter of 50-500A DEG until the content of soluble solid is 0.5-1.0%, adding 5-20 times of anhydrous ethanol, uniformly stirring, cooling to 0-4 ℃ to obtain an alcohol precipitation feed liquid;
s6, performing graded filtration on the alcohol precipitation liquid obtained in the step S5 by using 8000-10000-mesh polypropylene fiber cloth and filter paper with the aperture of 1-11 mu m, washing filter residues by using 5-15 times of absolute ethyl alcohol, and then performing vacuum drying to obtain light yellow polyacetyl glucosamine; vacuum concentrating the filtrate until the content of soluble solid is 3.0% -6.0%, adding anhydrous ethanol, standing for 24h to obtain flocculent precipitate, centrifuging, washing the filter cake with anhydrous ethanol, and vacuum drying to obtain Tremella polysaccharide.
2. The method for extracting functional ingredients from tremella as claimed in claim 1, wherein: in the step S1, the stirring time at room temperature is 10h, the centrifugal speed is 600r/min, and the centrifugal time is 60 min.
3. The method for extracting functional ingredients from tremella as claimed in claim 1, wherein: the addition amount of the pectinase and the acid cellulase in the step S3 is 0.5-1.5 per mill of the weight of the fresh tremella, and the enzymolysis temperature is 50 ℃.
4. The method for extracting functional ingredients from tremella as claimed in claim 1, wherein: the buffer aqueous solution in the step S4 is a buffer aqueous solution composed of inorganic salt and organic salt and having pH of 8-10, and the addition amount is 20 times of that of the white fungus residue.
5. The method for extracting functional ingredients from tremella as claimed in claim 1, wherein: the compound enzyme in the step S5 is obtained by mixing galacturonase and protease according to the mass ratio of 1:2-1:5, and the adding amount of the compound enzyme is 0.3-1 per mill of the mixed liquid obtained in the step S3 and the filtrate obtained in the step S4 after the filtrate is combined.
6. The method for extracting functional ingredients from tremella as claimed in claim 1, wherein: in the step S5, the specification of the polypropylene fiber cloth is 2500 meshes, and the pore size of the ultrafiltration membrane is 50-100A degrees.
7. The method for extracting functional ingredients from tremella as claimed in claim 1, wherein: the mesh number of the polypropylene fiber cloth in the step S6 is 10000 meshes.
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| CN114983893A (en) * | 2022-06-22 | 2022-09-02 | 可可琪可思曼中华有限公司 | Method and apparatus for preparing anti-sugar composition |
| CN116462778A (en) * | 2023-04-17 | 2023-07-21 | 浙江天草生物科技股份有限公司 | Extraction method of tremella polysaccharide |
| CN116462778B (en) * | 2023-04-17 | 2024-02-23 | 浙江天草生物科技股份有限公司 | Extraction method of tremella polysaccharide |
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