CN104072627A - Preparation method for typhonium giganteum polysaccharide extract - Google Patents
Preparation method for typhonium giganteum polysaccharide extract Download PDFInfo
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Abstract
The invention discloses a preparation method for typhonium giganteum polysaccharide extract, and relates to a preparation method for polysaccharide extract. The preparation method specifically comprises the following preparation steps of: adding 1-10% (in percent by weight) of compound enzyme and 3-15% of water into dried typhonium giganteum rhizome powder for carrying out enzymolysis under an ultrasonic condition; carrying out extraction/suction filtration under the ultrasonic condition; concentrating by a centrifugal machine, adopting 70-95% alcohol to stir and mix the concentrated liquor, carrying out alcohol precipitation for 6-18 hours, centrifuging for 5-20 minutes under rotation speed of 3000 revolutions/minute, collecting polysaccharides on the bottom of a centrifugal cup; and drying to obtain typhonium giganteum polysaccharide extract. The preparation method disclosed by the invention is simple and practical in operation, is high in extracting efficiency, and time for extracting typhonium giganteum polysaccharide by applying the process can be saved; and the extract has remarkable biological activities for preventing tyrosine kinase, oxidization, tumors, and the like.
Description
Technical field
The present invention relates to a kind of preparation method of polyoses extract, particularly relate to a kind of preparation method of Herba Typhonii gigantei polyoses extract.
Background technology
Herba Typhonii gigantei (Typhonium giganteum Engl.) is Araeceae (Araceae) Typhonium Schott (Typhonium Schott) plant, is the distinctive kind of plant of China.Herba Typhonii gigantei mainly originates in the ground such as Henan, Sichuan, Shaanxi, and existing all parts of the country are many cultivations also, and the Northeast's output is high, and price is cheaper." traditional herbal medicine ", " Chinese medicine voluminous dictionary " are recorded, and Herba Typhonii gigantei bulb hyoscine by cold-dampness, the phlegm that dispels the wind, antispastic, control apoplexy phlegm and stop up, facial hemiparalysis, tetanus, is controlled wound, lymphoid tuberculosis.Up to now, still less to the research of this kind of plant, only limit to folk prescription among the people and use, the research to its effective active composition and application are also in the development phase.
Polysaccharide is a kind of natural macromolecular material being extensively present in animal cell membrane and plant, microorganism wall, is a kind of important organism energy resource and constituent material, also participates in the various activities of cell simultaneously.Research shows, many vegetable polysaccharidess have immunomodulatory, antitumor, hypoglycemic, radioprotective and the biological activity such as delay senility.
Prove according to data check and applicant experimental study, the Herba Typhonii gigantei polysaccharide extracting from Herba Typhonii gigantei has the immunizing power of significantly improving, the function such as antitumor, anti-oxidant.Herba Typhonii gigantei polysaccharide is pure natural substance, can be prepared into healthcare products or medicine by forms such as foodstuff additive, medicinal granules, oral liquid, capsule, dripping pill, soft capsules.The paper that applicant is delivered use water extraction and alcohol precipitation method extract Herba Typhonii gigantei polysaccharide, Sevag method is except the purification process of albumen, but this extracting method exists extraction time long, high, the problem such as active component extract yield is low consumes energy.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of Herba Typhonii gigantei polyoses extract, the method comprises pretreatment, supersound extraction or microwave extraction, concentrated, alcohol precipitation, collection, dry, packaging is preserved etc., apply the Herba Typhonii gigantei polysaccharide saving extraction time that this technique is extracted, extraction yield is high, and extract has significant anti-Tyrosylprotein kinase, the biological activity such as anti-oxidant and antitumor.
The object of the invention is to be achieved through the following technical solutions:
A preparation method for Herba Typhonii gigantei polyoses extract, described method utilizes prozyme under ultrasound condition, medicinal material to be carried out enzymolysis, carries out supersound extraction, concentrated, alcohol precipitation, collection, dry making again, specifically comprises following preparation process:
1) in dry Herba Typhonii gigantei rhizome powder, add the prozyme of 1-10% weight ratio and the water of 3-15 weight ratio, at 30-90 DEG C, carry out enzymolysis 0.5-5h under the Ultrasonic Conditions of 25KHz-100KHz, enzymolysis finishes post-heating deactivation prozyme;
2) under the Ultrasonic Conditions of 25KHz-100KHz, carry out 1-4 time and extract, each 1-5h, united extraction liquid carries out suction filtration; By whizzer centrifugal 5-20 minute under the condition of 2000-4000 rev/min, handled thing is carried out to solid-liquid separation again, supernatant liquor is concentrated after abandoning solid substance;
3) supernatant liquor carries out concentrating under reduced pressure, and the 1/4-1/5 that is concentrated into original volume gets final product united extraction liquid;
4) adopt the ethanol of 70-95% concentration to be uniformly mixed above-mentioned concentrated solution, alcohol precipitation 6-18 hour, centrifugal 5-20 minute under 3000 revs/min of rotating speeds, collects Centrifuge Cup bottom polysaccharide;
5) dry, obtain Herba Typhonii gigantei polyoses extract product.
The preparation method of described a kind of Herba Typhonii gigantei polyoses extract, described prozyme is papoid, cellulase, polygalacturonase, aspartic protease, at, many phthaleins glycanase and diastatic two or more mixtures arbitrarily.
Advantage of the present invention and effect are:
The invention provides a kind of safety, environmental protection, efficient Herba Typhonii gigantei extraction method of polysaccharides, the method extraction yield is high, purity is high, use ultrasonic wave combined-enzyme method to extract Herba Typhonii gigantei polysaccharide, comprise the processing steps such as pretreatment, supersound extraction or microwave extraction, concentrated, alcohol precipitation, collection, dry, packaging preservation, this technological operation is simple and practical, apply the Herba Typhonii gigantei polysaccharide saving extraction time that this technique is extracted, extraction yield is high, and extract has significant anti-Tyrosylprotein kinase, the biological activity such as anti-oxidant and antitumor.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
In following embodiment, agents useful for same is commercially available prod, below in conjunction with embodiment, invention is elaborated.
Embodiment 1:
Prozyme (0.5g papoid, 0.5g cellulase and 0.5g amylase) is added in the Herba Typhonii gigantei rhizome powder that 50g is dry, adds water 200ml, in the ultrasonic generator of 25KHz, at 30 DEG C, ultrasonic enzymolysis 2h, after enzymolysis finishes, enzyme 1h goes out at 100 DEG C.Selected frequency is the Ultrasonic Conditions of 100KHz, extracts 2 times through pretreated extraction material above-mentioned, each 1h, united extraction liquid, by whizzer under the condition of 3000 revs/min centrifugal 10 minutes, handled thing is carried out to solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance; Carry out concentrating under reduced pressure at 60 DEG C to collecting supernatant liquor, be concentrated into 1/5 of original volume; Adopt the ethanol of 95% concentration to be uniformly mixed above-mentioned concentrated solution, concentrated solution alcohol precipitation is after 12 hours, under 3000 revs/min of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide; The polysaccharide of collection is put in vacuum drying oven, 50 DEG C of vacuum-dryings; Yield 3.1%, polysaccharide content 38.9%.
Embodiment 2:
Prozyme (1.0g cellulase and 0.5g polygalacturonase) is added in the Herba Typhonii gigantei rhizome powder that 50g is dry, adds water 250ml, in the ultrasonic generator of 25KHz, at 30 DEG C, ultrasonic enzymolysis 2h, after enzymolysis finishes, enzyme 1h goes out at 100 DEG C.Selected frequency is the Ultrasonic Conditions of 80KHz, extracts 2 times through pretreated extraction material above-mentioned, each 1h, united extraction liquid, by whizzer under the condition of 3000 revs/min centrifugal 10 minutes, handled thing is carried out to solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance; Carry out concentrating under reduced pressure at 60 DEG C to collecting supernatant liquor, be concentrated into 1/4 of original volume; Adopt the ethanol of 80% concentration to be uniformly mixed above-mentioned concentrated solution, concentrated solution alcohol precipitation is after 10 hours, under 3000 revs/min of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide; The polysaccharide of collection is put in vacuum drying oven, 50 DEG C of vacuum-dryings; Yield 3.5%, polysaccharide content 32.4%.
Embodiment 3:
Prozyme (1.0g cellulase and 0.5g amylase) is added in the Herba Typhonii gigantei rhizome powder that 50g is dry, adds water 500ml, in the ultrasonic generator of 25KHz, at 30 DEG C, ultrasonic enzymolysis 4h, after enzymolysis finishes, enzyme 1h goes out at 100 DEG C.Selected frequency is the Ultrasonic Conditions of 100KHz, extracts 2 times through pretreated extraction material above-mentioned, each 1h, united extraction liquid, by whizzer under the condition of 3000 revs/min centrifugal 10 minutes, handled thing is carried out to solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance; Carry out concentrating under reduced pressure at 60 DEG C to collecting supernatant liquor, be concentrated into 1/4 of original volume; Adopt the ethanol of 90% concentration to be uniformly mixed above-mentioned concentrated solution, concentrated solution alcohol precipitation is after 12 hours, under 3000 revs/min of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide; The polysaccharide of collection is put in vacuum drying oven, 50 DEG C of vacuum-dryings; Yield 3.3%, polysaccharide content 34.4%.
Embodiment 4:
Prozyme (0.5g aspartic protease and many phthaleins of 0.5g glycanase) is added in the Herba Typhonii gigantei rhizome powder that 50g is dry, adds water 300ml, in the ultrasonic generator of 25KHz, at 30 DEG C, ultrasonic enzymolysis 1h-4h, after enzymolysis finishes, enzyme 0.5-1h goes out at 100 DEG C.Selected frequency is the Ultrasonic Conditions of 100KHz, extracts 2 times through pretreated extraction material above-mentioned, each 1h, united extraction liquid, by whizzer under the condition of 3000 revs/min centrifugal 15 minutes, handled thing is carried out to solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance; Carry out concentrating under reduced pressure at 60 DEG C to collecting supernatant liquor, be concentrated into 1/4 of original volume; Adopt, the ethanol of 75% concentration is uniformly mixed above-mentioned concentrated solution, and concentrated solution alcohol precipitation is after 12 hours, under 3000 revs/min of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide; The polysaccharide of collection is put in vacuum drying oven, 50 DEG C of vacuum-dryings; Yield 3.1%, polysaccharide content 33.8%.
Claims (2)
1. a preparation method for Herba Typhonii gigantei polyoses extract, is characterized in that, described method utilizes prozyme under ultrasound condition, medicinal material to be carried out enzymolysis, carries out supersound extraction, concentrated, alcohol precipitation, collection, dry making again, specifically comprises following preparation process:
1) in dry Herba Typhonii gigantei rhizome powder, add the prozyme of 1-10% weight ratio and the water of 3-15 weight ratio, at 30-90 DEG C, carry out enzymolysis 0.5-5h under the Ultrasonic Conditions of 25KHz-100KHz, enzymolysis finishes post-heating deactivation prozyme;
2) under the Ultrasonic Conditions of 25KHz-100KHz, carry out 1-4 time and extract, each 1-5h, united extraction liquid carries out suction filtration; By whizzer centrifugal 5-20 minute under the condition of 2000-4000 rev/min, handled thing is carried out to solid-liquid separation again, supernatant liquor is concentrated after abandoning solid substance;
3) supernatant liquor carries out concentrating under reduced pressure, and the 1/4-1/5 that is concentrated into original volume gets final product united extraction liquid;
4) adopt the ethanol of 70-95% concentration to be uniformly mixed above-mentioned concentrated solution, alcohol precipitation 6-18 hour, centrifugal 5-20 minute under 3000 revs/min of rotating speeds, collects Centrifuge Cup bottom polysaccharide;
5) dry, obtain Herba Typhonii gigantei polyoses extract product.
2. the preparation method of a kind of Herba Typhonii gigantei polyoses extract according to claim 1, it is characterized in that, described prozyme is papoid, cellulase, polygalacturonase, aspartic protease, at, many phthaleins glycanase and diastatic two or more mixtures arbitrarily.
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| CN107540759A (en) * | 2017-09-29 | 2018-01-05 | 贵州中科健生物医药有限公司 | A kind of method that polysaccharide is extracted from the fruit of Chinese wolfberry |
| CN108892733A (en) * | 2018-05-25 | 2018-11-27 | 兰州大学 | A kind of application of hedysarum polybotys saccharide method of modifying and the hedysarum polybotys saccharide of modification |
| CN109369815A (en) * | 2017-08-08 | 2019-02-22 | 中国科学院上海药物研究所 | A kind of fructus lycii arabogalactan as well as preparation method and application thereof |
| CN109400730A (en) * | 2017-08-18 | 2019-03-01 | 中国科学院上海药物研究所 | A kind of polysaccharides, preparation method and the usage |
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| CN109369815A (en) * | 2017-08-08 | 2019-02-22 | 中国科学院上海药物研究所 | A kind of fructus lycii arabogalactan as well as preparation method and application thereof |
| CN109369815B (en) * | 2017-08-08 | 2021-05-28 | 中国科学院上海药物研究所 | Wolfberry fruit arabinogalactan and preparation method and application thereof |
| CN109400730A (en) * | 2017-08-18 | 2019-03-01 | 中国科学院上海药物研究所 | A kind of polysaccharides, preparation method and the usage |
| CN107540759A (en) * | 2017-09-29 | 2018-01-05 | 贵州中科健生物医药有限公司 | A kind of method that polysaccharide is extracted from the fruit of Chinese wolfberry |
| CN108892733A (en) * | 2018-05-25 | 2018-11-27 | 兰州大学 | A kind of application of hedysarum polybotys saccharide method of modifying and the hedysarum polybotys saccharide of modification |
| CN108892733B (en) * | 2018-05-25 | 2021-04-30 | 兰州大学 | Radix hedysari polysaccharide modification method and application of modified radix hedysari polysaccharide |
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Effective date of registration: 20190215 Address after: 110179 No. 6 Xinluo Street, Hunnan New District, Shenyang City, Liaoning Province Patentee after: Shenyang Hongqi Pharmaceutical Co., Ltd. Address before: No. 11, economic and Technological Development Zone, Shenyang, Liaoning Province, Liaoning Patentee before: Shenyang University of Chemical Technology |